Since the fluorescence intensity of rare events and the position of thousands of surrounding cells are recorded at the same ... Cells with the rare stem-cell like phenotype can be efficiently identified in the undisturbed cultures. ... the study of stem cell like cells in cultures of primary cells, cancer cells, and various cell lines. ... Combination of quantification and observation methods for study of "Side Population" cells in their "in vitro" microenvironment ...

... in side population cells and in non-side population cells that were isolated from HCC cell lines. (B) Sphere formation assay: ... Sorting and identification of side population cells in the human cervical cancer cell line HeLa. Cancer Cell International. ... Sorting and identification of side population cells in the human cervical cancer cell line HeLa. Cancer Cell International. ... SP-side population; NSP-non side population). (B) SP cells of MHCC-97H, Huh7, SMMC-7721 and HepG2 were analyzed by dual ...

... epithelial side population cells exhibit a distinct expression of stem cell markers that are lacking in side population cells ... "Myogenic specification of side population cells in skeletal muscle". The Journal of Cell Biology. 159 (1): 123-34. doi:10.1083/ ... Smalley MJ, Clarke RB (January 2005). "The mammary gland "side population": a putative stem/progenitor cell marker?". Journal ... Stem cell markers are genes and their protein products used by scientists to isolate and identify stem cells. Stem cells can ...

Isolation of side population cells in B-cell non-Hodgkins lymphomas. Mi Ran Lee, Hyun Jeong Ju, Byung Soo Kim, Young Hyeh Ko, ... Dive into the research topics of Isolation of side population cells in B-cell non-Hodgkins lymphomas. Together they form a ...

Stem Cells * Ovarian cancer stem cells are enriched in side population and aldehyde dehydrogenase bright overlapping population ... Ovarian cancer stem cells are enriched in side population and aldehyde dehydrogenase bright overlapping population. ... Ovarian cancer stem cells are enriched in side population and aldehyde dehydrogenase bright overlapping population. ... Ovarian cancer stem cells are enriched in side population and aldehyde dehydrogenase bright overlapping population. ...

Combined volasertib and TMZ treatment reduced side population (SP) indicating activity against GBM stem-like cells. Volasertib ... Volasertib alone and in combination with TMZ and/or XRT promoted apoptotic cell death, altered MtMP, increased ROS and G2/M ... We quantified the effects of volasertib alone and in combination with TMZ and/or XRT on GBM cell cytotoxicity/apoptosis, ... mitochondrial membrane potential (MtMP), reactive oxygen species (ROS), cell cycle, stemness, DNA damage, DNA repair genes, ...

Side population and CD marker assays for stem cells. *Direct cell cloning ... Lim has more than 18 years of experience in cell sorting, cell cycle analysis, apoptosis and immunophenotyping. Specific areas ... Ph.D. Cell and Molecular Biology, Colorado State University, 2004. M.S. Environmental and Radiological Health Sciences, ... of androgen-deprived prostate cancer cells. Oncotarget 2015 May 30; 6(15): 13088-104 ...

Cell-cultures; Author Keywords: Carbon nanotubes; lung fibrosis; stem-like fibroblasts; fibroblastic nodules; side population ... Induction of stemlike cells with fibrogenic properties by carbon nanotubes and its role in fibrogenesis. ... Nanotechnology; Models; Fibrogenicity; Cell-function; Cellular-function; Fibrogenesis; Humans; Men; Women; Lung; Lung-disease; ... fibroblastic nodule model for fibrogenicity testing of nanomaterials and investigated the role of fibroblast stemlike cells ( ...

... and quantitative RT-PCR of cardiac side population stem cells. Adult cardiac side population stem cells (CSPCs) were isolated ... in satellite cells (Allen et al., 1991), and in cardiac muscle side population stem cells (CSPCs) (Pfister et al., 2005). ... Using reporter-gene assays in different cell types and Nkx2.5 expression analysis in cardiac side population stem cells (CSPCs ... Absence of desmin expression in cardiac side population stem cells from adult desmin−/− mouse hearts causes reduced Nkx2.5 ...

The forward scatter versus side scatter area was used to analyze a homogeneous population of live cells. Samples were analyzed ... 2008) Apoptosis meets autophagy-like cell death in the ischemic penumbra: two sides of the same coin? Autophagy 4:422-426. ... B, Single-cell evaluation of cell surface GLUT3 expression in sham (1 h) and glutamate-treated neurons over a 24 h period. Data ... Cells were gated according to size. A right shift (gray-green) in distribution indicates an increase in GLUT3 cell surface ...

Properties of a pancreatic side-population stem cell lines from a patient with congenital hyperinsulinism. Sajib A , Kim J , ... of the ATP-binding cassette protein ABCG2 and to characterize the cells for their expression of markers of Side Population (SP ... NES2Y cells were derived several years ago from a patient following surgery for Congenital Hyperinsulinism (CHI). These cells ... the reduced cortical thickness and osteoblast differentiation were observed by QCT and in vitro cell culture, respectively. ...

We isolated tumor cell lines from a transgenic mouse model of anaplastic large cell lymphoma (ALCL), a rare T-cell lymphoma, ... Further, HDACs regulate T-cell development and HDAC inhibitors (HDACis) have been approved for clinical use in some T-cell ... Our data provide a comprehensive view of the targets of HDAC inhibition in malignant T cells with general applicability and ... cell death and differentiation and thus critically involved in tumorigenesis. ...

Journal Article] Identification of vascular endothelial side population cells in the choroidal vessels and their potential role ... In terms of malignant transformation in cancer cells, interaction of cancer stem cells with endothelial cells or myofibroblasts ... DNA damage enhanced by the attenuation of SLD5 delays cell cycle restoration in normal cells but not in cancer cells.2014. *. ... Presentation] Identification of endothelial stem cell population in the pre-existing blood vessels2011. *. Author(s). Nobuyuki ...

A subset of microRNAs defining the side population of a human malignant mesothelioma cell line.. Oncotarget. 2017; 8(26):42847- ... Total RNAs were isolated from the sorted side population and non-side population of MS1. The RNAs were subjected to analysis ... a total of 95 microRNAs were differentially expressed in the side population compared to the non-side population. Functional ... Flow cytometry was used to investigate cell apoptosis and cell cycle distribution.. RESULTS: DDIT4 was upregulated in GC cells ...

Anaplastic large cell lymphoma-propagating cells are detectable by side population analysis and possess an expression profile ... Cutaneous T cell lymphoma expresses immunosuppressive CD80 (B7-1) cell surface protein in a STAT5-dependent manner. J Immunol. ... We found that cutaneous T-cell lymphoma cells display activation of the interleukin-2 receptor/cytokine common chain-associated ... Stonecypher M, Yan Z, Wasik MA, LiVolsi V: Intravascular Large B-cell Lymphoma presenting as a thyroid mass. Endocrine Pathol. ...

It is present in 35% of the population. The degree of pneumatization may vary from side to side. Usually, 1 cell (and ... The Haller cell, or infraorbital cell, extends inferior to the ethmoid bulla and lateral to the maxillary sinus roof and ... A large Haller cell may obstruct the middle meatus. It is usually located in the anterior ethmoid, but it may extend all the ... The frontal sinus is projected obliquely, and the ethmoid air cells are obscured, although a few may be seen along the medial ...

A minor subpopulation of cancer cells, known as glioma stem-like cells (GSCs), are thought to play a major role in tumor ... relapse due to their stem cell-like properties, their high resistance to conventional treatments and their high invasion ... CD34 or side populations do not resume tumor-initiating properties of long-term cultured cancer stem cells from human malignant ... siCt TG1N cells (F) or shCt TG1N cells (G)). (I) Invasion in Matrigel chambers of ShJMY-TG1N cells and ShCt-TG1N cells after ...

Find a cell sorter that combines sorting capabilities with best-in-class biosafety expertise for a comprehensive advanced cell ... The flow cell is designed to improve resolution for side population applications and DNA cell cycle analyses. ... The BD FACSAria™ Fusion System has been used in resolving various types of cell populations. ... Complete cell surface phenotype of every cell sorted into a multiposition sort device, such as a 96-well tray. ...

Guidelines for the Performance of CD4+ T-Cell Determinations in Persons with Human Immunodeficiency Virus Infection ... b. Based on the above characteristics, draw a gate around the lymphocyte population (based on low forward- and side-scattering ... Note: CD4+ T-cells are T-helper cells. The correct cells to report for this value are those that are positive for both CD3 and ... d. Optimally, the sum of the %CD3+ (T-cells), %CD19+ (B-cells), and the %CD3-(CD16 and/or CD56)+ (NK-cells) should equal the ...

... circulating side population cells, and cytokines. Exp Hematol 2008;36:216-223. ... Mayack SR, Wagers AJ: Osteolineage niche cells initiate hematopoietic stem cell mobilization. Blood 2008;112:519-531. ... Effects of Exercise Training on Red Blood Cell Production: Implications for Anemia Subject Area: Hematology , Oncology ... Red Blood Cells Expressing Fetal Antigens: Their Presence in Adults with Certain Forms of Anemia Acta Haematol (February,2009) ...

The role of side populations (SP) or cancer stem-like cells (CSC) in promoting the resistance phenotype presents a viable ... C cells relative to doxorubicin-resistant cells. A sub-population of cells with CSC characteristics is enriched during ... such as cell growth, cell cycle, cell metastasis of hepatoma cells, and to discuss the TMEM9 gene‑encoding protein as a ... Cell-surface markers and side populations (SPs) were analyzed using flow cytometry. Differentially expressed stemness genes ...

Kaempferol and docetaxel diminish side population and down-regulate some cancer stem cell markers in breast cancer cell line ... After TS or ABCG2 in 5-FU-resistant breast cancer cells was knocked down, the sensitivity of SKBR-3/ FU and MDA-MB-453/FU cells ... 5-FU remarkably suppressed the growth of SKBR-3 and MAD-MB-453 cells. However, SKBR-3/FU and MADMB-453/FU cells showed ... inhibition of the growth of the 5-FU-resistant SKBR-3/FU and MDA-MB-453/FU cell lines and the parental cell lines. Pyrotinib ...

... and variable subsets of thymocytes and peripheral T cells. CD44 primarily functions as an adhesion molecule. It has been ... CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, ... Muscle CD31(-) CD45(-) side population cells promote muscle regeneration by stimulating proliferation and migration of ... CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, ...

A successful therapy must therefore eliminate these cells known to be highly resistant to apoptosis. In this paper, we describe ... the metabolic changes as well as the mechanisms of resistance to apoptosis occurring in cancer cells and cancer stem cells, ... Accumulating evidence shows that cancer stem cells are key drivers of tumor formation, progression, and recurrence. ... a portion of the cells, called side population (SP), remains unlabelled and may be isolated on this basis. This population ( ...

Side effects can include headaches and dizziness.. Dina Nurdybay, a Kazakh woman, was detained in a camp which separated ... When a visiting official peered through the iron bars of their cell, she recited her lines in halting Mandarin. ... The population control measures are backed by mass detention both as a threat and as a punishment for failure to comply. Having ... The growing Muslim population was a breeding ground for poverty and extremism, "heightening political risk," according to a ...

Integrated glycomic analysis of ovarian cancer side population cells.. Zhao, R., Liu, X., Wang, Y., Jie, X., Qin, R., Qin, W., ... Large-scale cell production of stem cells for clinical application using the automated cell processing machine. ... Purified and crude samples, e.g. cell/tissue extracts, living cells, virus particles, cell culture supernatants, serum, urine ... cell/tissue extracts, living cells, virus particles, cell culture supernatants, serum, urine). Compared to MS-based methods, it ...

Then, we will discuss several principles that govern macrophage phagocytosis of apoptotic cells (efferocytosis), including the ... lineage and population maintenance of microglia and macrophages. Then, we will discuss several principles that govern ... lineage and population maintenance of microglia and macrophages. ... in the form of dead cells and protein aggregates. To prevent ... macrophage phagocytosis of apoptotic cells (efferocytosis), including the existence of redundant recognition mechanisms ( ...

... compaction hypersensitivity may be widespread among damaged cells and offers an additional route to eliminate unfit cells. Cell ... How cells compete is poorly understood, but it is generally accepted that molecular exchange between cells signals elimination ... Here we report an orthogonal mechanism of cell competition, whereby cells compete through mechanical insults. We show that MDCK ... causing cell death. Thus, in addition to molecules, cells use mechanical means to compete. Given the involvement of p53, ...

Nicotine promotes apoptosis resistance of breast cancer cells and enrichment of side population cells with cancer stem cell- ... Side-population cells in luminal-type breast cancer have tumour-initiating cell properties, and are regulated by HER2 ... The importance of HER2 signaling in the tumor-initiating cell population in aromatase inhibitor-resistant breast cancer. Breast ... Chumsri S, Shah P. Radiation Resistance of Cancer Stem Cells as an Obstacle in Cancer Therapy. Mol Cell Pharmacol. 2013; 5(1): ...