*  Retinas of the Diurnal Rodent Arvicanthis ansorgei Are Highly Resistant to Experimentally Induced Stress and Degeneration |...
49 and Williams and Howell 14 all pointed to rhodopsin bleaching as the trigger for retinal light damage. Both rhodopsin and ... NSD between +ILE and −ILE (n = 1) retinas as revealed by DAPI staining (A, D), rhodopsin (B, E), and cone MW opsin (C, F) ... NSD between +ILE and −ILE (n = 1) retinas as revealed by DAPI staining (A, D), rhodopsin (B, E), and cone MW opsin (C, F) ... NSD between +ILE and −ILE (n = 1) retinas as revealed by DAPI staining (A, D), rhodopsin (B, E), and cone MW opsin (C, F) ...
https://iovs.arvojournals.org/article.aspx?articleid=2186888
*  Genotype and Phenotype Studies in Autosomal Dominant Retinitis Pigmentosa (adRP) of the French Canadian Founder Population |...
Gene abbreviations: rhodopsin (RHO); small nuclear ribonucleoprotein 200 kDa (U5) (SNRNP200); peripherin 2 (PRPH2/RDS); the ... Gene abbreviations: rhodopsin (RHO); small nuclear ribonucleoprotein 200 kDa (U5) (SNRNP200); peripherin 2 (PRPH2/RDS); the ... Spectrum of rhodopsin mutations in French autosomal dominant rod-cone dystrophy patients. Invest Ophthalmol Vis Sci. 2010; 51: ... Gene abbreviations: rhodopsin (RHO); small nuclear ribonucleoprotein 200 kDa (U5) (SNRNP200); peripherin 2 (PRPH2/RDS); the ...
http://iovs.arvojournals.org/article.aspx?articleid=2479673
*  Formation and Clearance of All-Trans-Retinol in Rods Investigated in the Living Primate Eye With Two-Photon Ophthalmoscopy |...
Rod Outer Segment Retinol Formation Is Independent of Abca4, Arrestin, Rhodopsin Kinase, and Rhodopsin Palmitylation ... trans-retinol is related to rhodopsin bleaching: clearly, for light exposures that completely bleach rhodopsin, the quantity of ... The fraction of rhodopsin pigment bleached as a consequence of the brief flashes delivered to the retina was calculated by ... The fraction of rhodopsin pigment bleached as a consequence of the brief flashes delivered to the retina was calculated by ...
http://iovs.arvojournals.org/article.aspx?articleid=2600148
*  Loss of Citron Kinase Affects a Subset of Progenitor Cells That Alters Late but Not Early Neurogenesis in the Developing Rat...
A-B') Immunohistochemistry on P7 retinal sections showing rhodopsin (green) and Pax6 (red) in WT (A) and KO (A'); and Islet1 ( ... A-B') Immunohistochemistry on P7 retinal sections showing rhodopsin (green) and Pax6 (red) in WT (A) and KO (A'); and Islet1 ( ... A-B') Immunohistochemistry on P7 retinal sections showing rhodopsin (green) and Pax6 (red) in WT (A) and KO (A'); and Islet1 ( ... A-B') Immunohistochemistry on P7 retinal sections showing rhodopsin (green) and Pax6 (red) in WT (A) and KO (A'); and Islet1 ( ...
https://iovs.arvojournals.org/article.aspx?articleid=2212833
*  Apo-opsin forms a photoactivated rhodopsin-like state | IOVS | ARVO Journals
Apo-opsin forms a photoactivated rhodopsin-like state Shinya Sato; Beata Jastrzebska; Andreas Engel; Krzysztof Palczewski; ... Apo-opsin forms a photoactivated rhodopsin-like state You will receive an email whenever this article is corrected, updated, or ... Resistance of this 11-cis-7-ring rhodopsin to photoactivation and bleaching was confirmed biochemically. The signaling of the ... We observed similar events in RPE65-/- rods regenerated with the unbleachable rhodopsin analogue, further ruling out the ...
https://iovs.arvojournals.org/article.aspx?articleid=2642399
*  A Novel Potentially Causative Variant of NDUFAF7 Revealed by Mutation Screening in a Chinese Family With Pathologic Myopia |...
Identification of novel rhodopsin mutations responsible for retinitis pigmentosa: implications for the structure and function ... 8). When exposed to light, 11-cis-retinal is converted to all-trans-retinal, causing a conformational change in rhodopsin, ... Malanson KM, Lem J. Chapter 1: rhodopsin-mediated retinitis pigmentosa. In: Bouchard C, ed. Progress in Molecular Biology and ... Stojanovic A, Hwa J. Rhodopsin and retinitis pigmentosa: shedding light on structure and function. Receptors Channels. 2002; 8 ...
https://iovs.arvojournals.org/article.aspx?articleid=2650870
*  Potential of Small Molecule-Mediated Reprogramming of Rod Photoreceptors to Treat Retinitis Pigmentosa | IOVS | ARVO Journals
H) Rhodopsin expression was normalized to β-actin expression. Photoregulin1 decreased the relative expression of rhodopsin ... H) Rhodopsin expression was normalized to β-actin expression. Photoregulin1 decreased the relative expression of rhodopsin ... Western blot analysis shows that PR1-treated RhoP23H retinas have less Rhodopsin expression than DMSO controls. (B) Rhodopsin ... Western blot analysis shows that PR1-treated RhoP23H retinas have less Rhodopsin expression than DMSO controls. (B) Rhodopsin ...
https://iovs.arvojournals.org/article.aspx?articleid=2588569
*  Study of Rod- and Cone-Driven Oscillatory Potentials in Mice | IOVS | ARVO Journals
Evaluation of the rhodopsin knockout mouse as a model of pure cone function. Invest Ophthalmol Vis Sci. 2001;42:506-513. [ ... The electroretinogram of the rhodopsin knockout mouse. Vis Neurosci. 1999;16:391-398. [PubMed] ... and rhodopsin knockout (rho − / −) mouse. A Butterworth filter was used to extract OPs from ERG signals. Latencies were ... The original functional pure cone 13 14 rhodopsin knockout mice (rho − / −) were generously provided by Peter Humphries ( ...
http://iovs.arvojournals.org/article.aspx?articleid=2125868
*  A Novel Dominant Mutation in SAG, the Arrestin-1 Gene, Is a Common Cause of Retinitis Pigmentosa in Hispanic Families in the...
Rods are sensitive to a single photon of light,38 and it is crucial for the signaling cascade driven by rhodopsin, a G protein- ... Mutations within the rhodopsin gene in patients with autosomal dominant retinitis pigmentosa. N Engl J Med. 1990; 323: 1302- ... Each rhodopsin molecule binds its own arrestin. Proc Natl Acad Sci U S A. 2007; 104: 3125-3128. ... Note that the side chain is facing inward and Cys143 is localized near the rhodopsin-binding finger loop and the 139-loop (also ...
https://iovs.arvojournals.org/article.aspx?articleid=2629943
*  The Age-Regulating Protein Klotho Is Vital to Sustain Retinal Function | IOVS | ARVO Journals
As reports vary to whether rhodopsin levels fluctuate in the normal aging retina, 32,33 we examined rhodopsin levels by IHC and ... rhodopsin (purified 1D4, 0.1 μg/mL), transducin (1:2000, SC-389; Santa Cruz Biotechnology, Santa Cruz, CA), and rhodopsin ... Representative rhodopsin IHC images and quantification. Quantification of the rhodopsin signal in OS was conducted across ... Representative rhodopsin IHC images and quantification. Quantification of the rhodopsin signal in OS was conducted across ...
https://iovs.arvojournals.org/article.aspx?articleid=2202958
*  Altered Expression of Retinal Molecular Markers in the Canine RPE65 Model of Leber Congenital Amaurosis | IOVS | ARVO Journals
Li ZY Wong F Chang JH . Rhodopsin transgenic pigs as a model for human retinitis pigmentosa. Invest Ophthalmol Vis Sci. 1998;39 ...
https://iovs.arvojournals.org/article.aspx?articleid=2127419
*  Murine Retinal Progenitor Cells in the Ciliary Body Epithelium May Participate in Neurogenesis of the Peripheral Retina in vivo...
Rhodopsin was evenly expressed throughout the cell surface including the cell body with the outer segments being randomly ... By P9, majority of the BrdU- and rhodopsin-double-positive cells that had outer segment-like structures aligned at the cilio- ... Some of the cells double-positive for rhodopsin and BrdU in the CBE were morphologically identical to the rod photoreceptors in ... We also found that, in the developing murine retina, the pattern of rhodopsin expression within the photoreceptors and the ...
https://iovs.arvojournals.org/article.aspx?articleid=2386758
*  Minocycline Protects Photoreceptors from Light and Oxidative Stress in Primary Bovine Retinal Cell Culture | IOVS | ARVO...
3A 3B 3C)or with rhodopsin and recoverin antibodies (Figs. 3M 3N 3O) , or with a cell viability stain (Live/Dead Fixable Dead ... Most cells immunolabeled with anti-AIF are not rhodopsin positive (data not shown). It is not clear whether AIF played a role ... A-L) Cells were double immunolabeled either with recoverin (red) + PKCα (green); or with rhodopsin (yellowish green) + ... A-L) Cells were double immunolabeled either with recoverin (red) + PKCα (green); or with rhodopsin (yellowish green) + ...
https://iovs.arvojournals.org/article.aspx?articleid=2164039
*  Photoreceptor Layer Thickness Changes During Dark Adaptation Observed With Ultrahigh-Resolution Optical Coherence Tomography |...
... rhodopsin bleach) to the control (0% rhodopsin bleach) response. ... rhodopsin bleach) to the control (0% rhodopsin bleach) response ... rhodopsin bleach) to the control (0% rhodopsin bleach) response. ... rhodopsin bleach) to the control (0% rhodopsin bleach) response ... The left eyes of the five subjects were imaged with the half-field bleach at 54% rhodopsin bleach exposure to determine if the ... Thickness difference maps from a single subject at selected time points after the 54% half-field rhodopsin bleach. A 2D ...
https://iovs.arvojournals.org/article.aspx?articleid=2654080
*  A Mutation in Syne2 Causes Early Retinal Defects in Photoreceptors, Secondary Neurons, and Müller Glia | IOVS | ARVO Journals
4H). Rhodopsin staining at P5 was restricted to the developing IS-OS region in both mutants and controls (Figs. 6E, 6F). At P8 ... G, H) Colocalization of rhodopsin (green) and recoverin (red) show rod photoreceptors displaced from their normal position in ... G, H) Colocalization of rhodopsin (green) and recoverin (red) show rod photoreceptors displaced from their normal position in ... G, H) Colocalization of rhodopsin (green) and recoverin (red) show rod photoreceptors displaced from their normal position in ...
http://iovs.arvojournals.org/article.aspx?articleid=2323619
*  Quantitative Fundus Autofluorescence and Optical Coherence Tomography in PRPH2/RDS- and ABCA4-Associated Disease Exhibiting...
... rhodopsin was bleached in AF mode for 20 to 30 seconds21; detector sensitivity was adjusted so that the GLs were within the ...
https://iovs.arvojournals.org/article.aspx?articleid=2296459
*  VAMP7 as a regulator of rhodopsin transport carrier fusion in rod photoreceptors | IOVS | ARVO Journals
Purpose : The identity of the R-SNARE involved in the fusion of rhodopsin transport carriers (RTC) that pairs with Qabc-SNAREs ... Based on immunostaining, VAMP7 also co-localizes with rhodopsin, both in the Golgi/TGN and on RTCs. The localization of the ... Vasundhara Kandachar, Beatrice M Tam, Orson L Moritz, Dusanka Deretic; VAMP7 as a regulator of rhodopsin transport carrier ... VAMP7 as a regulator of rhodopsin transport carrier fusion in rod photoreceptors ...
https://iovs.arvojournals.org/article.aspx?articleid=2560307
*  Mimicking Retinal Development and Disease With Human Pluripotent Stem Cells | IOVS | ARVO Journals
Rhodopsin expression in the zebrafish pineal gland from larval to adult stage. Brain Res. 2012; 1442: 9-14. ... A subsequent study using an hiPSC line containing a RHODOPSIN missense mutation examined disease pathophysiology in greater ... or RHODOPSIN genes. Comparison of cell survival in RP versus normal hiPSC 2D cultures showed higher rates of rod death in the ...
https://iovs.arvojournals.org/article.aspx?articleid=2518372
*  CERKL Mutations Cause an Autosomal Recessive Cone-Rod Dystrophy with Inner Retinopathy | IOVS | ARVO Journals
Milam AH Li ZY Cideciyan AV Jacobson SG . Clinicopathologic effects of the Q64ter rhodopsin mutation in retinitis pigmentosa. ... Aleman TS Cideciyan AV Sumaroka A . Retinal laminar architecture in human retinitis pigmentosa caused by Rhodopsin gene ...
https://iovs.arvojournals.org/article.aspx?articleid=2185520
*  CNTF Negatively Regulates the Phototransduction Machinery in Rod Photoreceptors: Implications for Light-Induced Photostasis...
CNTF induces a decrease in rhodopsin expression and an increase in arrestin level. Similar changes were found in light exposed ...
https://iovs.arvojournals.org/article.aspx?articleid=2387593
*  Investigating the utility of mice as a model for developing clinically relevant dual AAV vectors to treat USH1B | IOVS | ARVO...
Our results suggest that MYO7A does not play a role in rhodopsin trafficking in mouse. In agreement with this, we see a ... Methods : We assessed whether the absence of MYO7A significantly impacted rhodopsin trafficking in mouse PRs by breeding newly ... Results : The absence of MYO7A had no impact on rhodopsin-GFP trafficking. Additionally, no differences were observed in ... Conclusions : Localization of rhodopsin cannot serve as a metric for evaluating therapeutic efficacy in Myo7a KO mice. ...
http://iovs.arvojournals.org/article.aspx?articleid=2641239
*  Retinal Ganglion Cells Recognized by Serum Autoantibody against γ-Enolase Found in Glaucoma Patients | IOVS | ARVO Journals
Anti-rhodopsin antibodies in sera from patients with normal-pressure glaucoma. Invest Ophthalmol Vis Sci. 1995;36:1968-1975. [ ... In addition, it was recently suggested that autoantibodies directed toward retinal antigens, such as rhodopsin, 9 60-kDa heat ... 14 and presence of serum antibodies toward rhodopsin, 9 heat shock proteins including hsp 60, 10 hsp 27, andα -crystallin 11 ... no immunoreactivities toward rhodopsin, α-crystalline, hsp 27, and hsp 60 were recognized. Although the serum antibody toward ...
https://iovs.arvojournals.org/article.aspx?articleid=2123542
*  Phosphodiesterase Inhibitors Sildenafil and Vardenafil Reduce Zebrafish Rod Photoreceptor Outer Segment Shedding | IOVS | ARVO...
However, the ROS that are present during early rod development are filled with light-sensing Rhodopsin,47,48 and they first ... Light activates Rhodopsin, a G-protein-coupled receptor, which initiates a process that leads to activation of ... Retinal sections were immunolabeled with anti-GFP (green), anti-HA (red), and anti-Rhodopsin (blue) antibodies. Images are ... Retinal sections were immunolabeled with anti-GFP (green), anti-HA (red), and anti-Rhodopsin (blue) antibodies. Images are ...
http://iovs.arvojournals.org/Article.aspx?articleid=2661288
*  Rhodopsin organization in retinal membranes of heterozygous rhodopsin knockout mice assessed by atomic force microscopy | IOVS ...
Like rhodopsin in WT mice, rhodopsin forms nanodomains in disc membranes of Rho+/- mice. The spatial density of rhodopsin in 4- ... Conclusions: Reduced rhodopsin expression reduces the size of ROS discs. Initially, a reduction in the expression of rhodopsin ... Rhodopsin organization in retinal membranes of heterozygous rhodopsin knockout mice assessed by atomic force microscopy ... Rhodopsin organization in retinal membranes of heterozygous rhodopsin knockout mice assessed by atomic force microscopy ...
https://iovs.arvojournals.org/article.aspx?articleid=2334395