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*  RNA expression patterns tied to breast cancer prognosis : Next Gen SEQ Report
RNA expression patterns of cancer-adjacent breast tissue could be to gauge future survival outcomes for women with estrogen receptor-positive breast cancer
  http://nextgenseqreport.com/rna-expression-patterns-tied-breast-cancer-prognosis/
*  Figure 3 from CCM2 expression parallels that of CCM1. - Semantic Scholar
Figure 3. Western blots of Cos7 cell extracts stained with CCM2 antibody (A and B). Cells were transfected with an expression vector encoding a full-length CCM2-GFP fusion protein ( ). Untransfected cells served as controls ( ). A, In transfected cells, the CCM2 antibody detects an 82-kDa protein (expected size for the fusion protein). No staining is observed in untransfected controls. B, Peptide competition eliminates staining, demonstrating the specificity of this antibody for the CCM2 protein. C, Multitissue Western blot reveals CCM2 protein expression in the brain, heart, lung, and kidney. - 'CCM2 expression parallels that of CCM1.'
  https://www.semanticscholar.org/paper/CCM2-expression-parallels-that-of-CCM1-%C5%9Eeker-Pricola/1b18f8ade8ed4ff67931cfd18bf22d976950435a/figure/2
*  KAKEN - Research Projects | A Study on the Cytokine Messenger RNA Expression in Bronchial Mucosal Biopsies from Patients with...
Principal Investigator:FUKUDA Takeshi, Project Period (FY):1991 - 1993, Research Category:Grant-in-Aid for General Scientific Research (C), Research Field:Respiratory organ internal medicine
  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670408/
*  Plus it
Gene ablation and reconstitution data show that PAI1 can promote cancer progression (11-14), and correlative data consistently indicate that its elevated expression in breast tumors is an independent indicator of poor prognosis (4-8). Here, we found that high PAI1 mRNA expression is also associated with shorter overall survival in breast cancer. Nevertheless, the transcription-altering PAI1 4G/5G SNP was not associated with breast cancer incidence, outcome, or tumoral PAI1 mRNA expression. Thus, local factors seem to be more important than germ line genetic variability in determining the level of PAI1 expression in breast cancer. Furthermore, our data show that PAI1 mRNA levels correlate with CTGF and the level of PAI1 gene amplification, suggesting that they play an important role in regulating the expression and adverse effects of PAI1 in breast cancer.. Because PAI1 mRNA expression correlates with its protein expression in breast cancer ...
  http://cebp.aacrjournals.org/content/15/11/2107
*  Polysomes from Yeast: Distribution of Messenger RNA and Capacity to Support Protein Synthesis in vitro | Science
Individual fractions of polysomes were isolated from yeast. Pulse labeling experiments in vivo show constant specific activity of messenger RNA in each polysome peak; this suggests a uniform density of ribosomes per unit length of messenger RNA. In the cell-free incorporating system, the amount of peptide per ribosome unit increased with the size of polysome.. ...
  http://science.sciencemag.org/content/147/3658/615
*  PHYSICAL HEALTH
Shinde and Klein are not yet sure whether GSK-3's effect on RNA splicing explains its role in mood disorders. The effect of GSK-3 on messenger RNA in neuronal cells, with or without lithium, would need to be examined to determine this. The study underlines how investigations into the basic biological function of a drug target can lead in unexpected directions. "[The GSK-3 phosphoproteome] is a really large data set," Shinde said. "It's a resource for the field." "The relevance to leukemia could be direct and something worthy of immediate study," Klein said. "The role in psychiatric disorders is a major interest of the work, but the impact would be down the road, not immediate." ...
  http://www.soothingemotions.com/physical-health
*  Time-dependent induction of connected cells by overexpr | Open-i
Time-dependent induction of connected cells by overexpression of cdc5ΔN. (A) Strain KLY1083 expressing three copies of GAL1-EGFP-cdc5ΔN homogeneously induced
  https://openi.nlm.nih.gov/detailedresult.php?img=PMC2195991_JCB0004052.f3ab&req=4
*  Messenger RNA
... (mRNA) is a molecule of RNA encoding a chemical "blueprint" for a protein product. mRNA is transcribed from a DNA template, and carries coding information to the sites of protein synthesis: the ribosomes. Here, the nucleic acid polymer is translated into a polymer of amino acids: a protein. In mRNA as in DNA, genetic information is encoded in the sequence of nucleotides arranged into codons consisting of three bases each. Each codon encodes for a specific amino acid, except the stop codons that terminate protein synthesis. This process requires two other types of RNA: transfer RNA (tRNA) mediates recognition of the codon and provides the corresponding amino acid, while ribosomal RNA (rRNA) is the central component of the ribosome's protein manufacturing machinery.. ...
  https://www.princeton.edu/~achaney/tmve/wiki100k/docs/Messenger_RNA.html
*  Semiquantitative RT-PCR measurement of gene expression in rat tissues including a correction for varying cell size and number
Background: Current methodology of gene expression analysis limits the possibilities of comparison between cells/tissues of organs in which cell size and/or number changes as a consequence of the study (e.g. starvation). A method relating the abundance of specific mRNA copies per cell may allow direct comparison or different organs and/or changing physiological conditions. Methods: With a number of selected genes, we analysed the relationship of the number of bases and the fluorescence recorded at a present level using cDNA standards. A lineal relationship was found between the final number of bases and the length of the transcript. The constants of this equation and those of the relationship between fluorescence and number of bases in cDNA were determined and a general equation linking the length of the transcript and the initial number of copies of mRNA was deduced for a given pre-established fluorescence setting. This allowed the calculation of the concentration of the ...
  http://www.recercat.cat/handle/2072/49484
*  A low number of SIC1 mRNA molecules ensures a low noise level in cell cycle progression of budding yeast - Molecular BioSystems...
The budding yeast genome comprises roughly 6000 genes generating a number of about 10 [ ] 000 mRNA copies, which gives a general estimation of 1-2 mRNA copies generated per gene. What does this observation implicate for cellular processes and their regulation? Whether the number of mRNA molecules produced is imp
  http://pubs.rsc.org/en/Content/ArticleLanding/2011/MB/c1mb05073g
*  SMART: Secondary literature for Ala racemase C domain
Mouse monocytic Mm-A, Mm-P, Mm-S1, and Mm-S2 cells are sublines of mouse monocytic and immortalized Mm-1 cells derived from spontaneously differentiated, mouse myeloblastic M1 cells. Although these subline cells retain their monocytic characteristics in vitro, Mm-A and Mm-P cells are highly leukemogenic to syngeneic SL mice and athymic nude mice, whereas Mm-S1 and Mm-S2 cells are not or are only slightly leukemogenic. To better understand the molecular mechanisms of these levels of leukemogenicity, we investigated putative leukemogenesis-associated genes or oncogenes involved in the maintenance of growth, especially in vivo, by means of differential mRNA display. We isolated a fragment clone (15T01) from Mm-P cells. The mRNA probed with 15T01 was expressed at high levels in leukemogenic Mm-P and Mm-A cells but not in nonleukemogenic Mm-S1 and Mm-S2 cells. The gene corresponding to 15T01, named TRA1, was isolated from an Mm-P cDNA library. The longest open reading frame of ...
  http://smart.embl-heidelberg.de/smart/show_secondary.cgi?domain=Ala_racemase_C
*  SMART: Secondary literature for RPOL9 domain
Mouse monocytic Mm-A, Mm-P, Mm-S1, and Mm-S2 cells are sublines of mouse monocytic and immortalized Mm-1 cells derived from spontaneously differentiated, mouse myeloblastic M1 cells. Although these subline cells retain their monocytic characteristics in vitro, Mm-A and Mm-P cells are highly leukemogenic to syngeneic SL mice and athymic nude mice, whereas Mm-S1 and Mm-S2 cells are not or are only slightly leukemogenic. To better understand the molecular mechanisms of these levels of leukemogenicity, we investigated putative leukemogenesis-associated genes or oncogenes involved in the maintenance of growth, especially in vivo, by means of differential mRNA display. We isolated a fragment clone (15T01) from Mm-P cells. The mRNA probed with 15T01 was expressed at high levels in leukemogenic Mm-P and Mm-A cells but not in nonleukemogenic Mm-S1 and Mm-S2 cells. The gene corresponding to 15T01, named TRA1, was isolated from an Mm-P cDNA library. The longest open reading frame of ...
  http://smart.embl-heidelberg.de/smart/show_secondary.cgi?domain=RPOL9
*  Mad2 phosphorylation regulates its association with Mad1 and the APC/C | The EMBO Journal
The stably expressed Mad2 proteins were immunoprecipitated from cell extracts with anti‐Flag antibody, and the co‐immunoprecipitated Cdc27 and Mad1 were visualized by western blot analysis (Figure 6B). Cdc20 co‐migrates with the antibody heavy chain, which makes it difficult to visualize Cdc20 by western blot analysis in co‐immunoprecipitations. Therefore, the same extracts were subjected to an immunoprecipitation with Cdc20 antibody, followed by a Mad2 western blot. Analysis of the interaction profiles of the stably expressed Mad2 constructs in cycling cells reveals that 3S‐D Flag‐Mad2 is unable to associate with Cdc27 (Figure 6B, lane 7) and Mad1 (Figure 6B, lane 11). The interaction with Cdc20 is also impaired (Figure 6B, lane 15). Similar results were observed in transient transfection assays (Figure 6G, lane 7 and 11). Finally, even though the protein amounts of 3S‐A Flag‐Mad2 are lower than those of Flag‐Mad2 (Figure 6B, compare lanes 2 and 4), a stronger interaction with ...
  http://emboj.embopress.org/content/22/4/797
*  Gene Expression Literature Detail
J:60127 Pascolo S, Tsoukatou D, Mamalaki C, Identification of thymus specific and developmentally regulated genes by an improved version of the mRNA differential display technique. Dev Immunol. 1999;7(1):1-7 ...
  http://www.informatics.jax.org/gxdlit/key/17329
*  RNApathwaysDB - a database of RNA maturation and decay.
SUBUNIT STRUCTURE:. The PAN deadenylation complex is a heterodimer of a catalytic subunit PAN2 and a regulatory subunit PAN3.. ENZYME REGULATION:. Positively regulated by the regulatory subunit PAN3. Negatively regulated by PAB1-binding protein PBP1. Inhibited under stress conditions. Inhibition of deadenylation under stress increases mRNA stability, which may be a mechanism to retain the majority of the cytoplasmic pool of mRNAs for later reuse and recovery from stress.. ...
  http://www.genesilico.pl/rnapathwaysdb/proteins/105/
*  SciCombinator - Systematic discovery of structural elements governing stability of mammalian messenger RNAs.
Comments, concepts and statistics about 'Systematic discovery of structural elements governing stability of mammalian messenger RNAs.'.
  http://scicombinator.com/articles/16666
*  Biology-Online • View topic - The Fiber Disease
DNA is the informational basis from which living cells derive instructions for synthesizing proteins. Many of the resulting proteins are enzymes that catalyze biochemical reactions from which the cell derives energy or generates other molecules essential to its health and safety. The process normally occurs when the sequence of nucleotides in DNA is transcribed into a complementary, single strand of nucleotides known as messenger RNA, or mRNA. The mRNA provides the instructions by which other components in the cell synthesize proteins. Because not all genes are transcribed (or expressed) but all genes that are transcribed do so through mRNA, the presence of mRNA is an indicator that a gene from the cell's DNA has been expressed. The DNA from which the mRNA is obtained is sometimes interspersed with oligonucleotide spacers that do not appear in the final mRNA. Because ...
  http://www.biology-online.org/biology-forum/about1958.html?p=58648&hilit=Water+aloe
*  curevac.de: curevac.com
CureVac is the first company to successfully harness messenger RNA (mRNA) for medical purposes. The principle is promising: use natural mRNA as a data carrier to instruct the human body to produce its own proteins to fight a wide range of diseases. ...
  http://www.curevac.com
*  FA page 199 G-protein linked 2nd messengers, major functions plus major grouping Flashcards - Cram.com
Study Flashcards On FA page 199 G-protein linked 2nd messengers, major functions plus major grouping at Cram.com. Quickly memorize the terms, phrases and much more. Cram.com makes it easy to get the grade you want!
  http://www.cram.com/flashcards/fa-page-199-g-protein-linked-2nd-messengers-major-functions-plus-major-grouping-353936