*  Small RNA Detection by in Situ Hybridization Methods - pdf descargar
Small RNA Detection by in Situ Hybridization Methods. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
*  Comparison of Fluorescence In Situ Hybridization and Chromogenic In Situ Hybridization for Low and High Throughput HER2 Genetic...
The purpose was to evaluate and compare 5 different HER2 genetic assays with different characteristics that could affect the performance to analyze the human epidermal growth factor 2 (HER2) gene copy number under low and high throughput conditions. The study included 108 tissue samples from breast cancer patients with HER2 immunohistochemistry (IHC) results scored as 0/1+, 2+, and 3+. HER2 genetic status was analysed using chromogenic in situ hybridization (CISH) and fluorescence in situ hybridization (FISH). Scoring results were documented through digital image analysis. The cancer region of interest was identified from a serial H&E stained slide following tissue cores were transferred to a tissue microarrays (TMA). When using TMA in a routine flow, all patients will be tested for HER2 status with IHC followed by CISH or FISH, thereby providing individual HER2 results. In conclusion, our results show that the differences between the HER2 genetic assays do not have an ...
*  In Situ Hybridization - Application Of The Probe For Dna Or Rna To Tissues Or Cells - Chromosome, Location, and Detect -...
In situ hybridization allows us to learn more about the geographical location of, for example, the messenger RNA (mRNA) in a cell or tissue. It can also tell us where a gene is located on a chromosome. Obviously, a detection system must be built into the technique to allow the cytochemist to visualize and map the geography of these molecules in the cells in question.. When in situ hybridization was first introduced, it was applied to isolated cell nuclei to detect specific DNA sequences. Early users applied the techniques to isolated chromosomal preparations in order to map the location of genes in those chromosomes. The technique has also been used to detect viral DNA in an infected cell. In situ hybridization of RNA has also been used to show that RNA synthesis (transcription) occurs in the nucleus, while protein synthesis (translation) occurs in the cytoplasm.. ...
*  Variant three-way translocation of inversion 16 in AML-M4Eo confirmed by fluorescence in situ hybridization analysis
All information about the latest scientific publications of the Clínica Universidad de Navarra. Variant three-way translocation of inversion 16 in AML-M4Eo confirmed by fluorescence in situ hybridization analysis
*  Antisense probe as negative control for in situ hybridization - Molecular Biology
Hi guys! So I'm doing some in situ hybridization experiments, and I want to design a better negative control, a labelled probe that shouldn't bind to the mRNA transcribed from my gene of interest.. I'm not trained as a molecular biologist, but I am keen to learn these things! So to hybridize to the mRNA of my gene, I design primers that are going to amplify a bit of DNA that is complementary to it, and then in vitro transcribe RNA that is complementary to that, and so it binds to the mRNAs of my gene, yeah?. How would I go about designing an anti-sense probe? What would this bind to? What should I amplify off of (i.e. what should the primers anneal to?)?. Any help or clues would be much appreciated. Gracias!. ...
*  Fluorescence In Situ Hybridization (FISH) | Thermo Fisher Scientific
Multiplex fluorescence in situ hybridization (FISH) enables you to assay multiple targets and visualize colocalized signals in a single specimen. Using spectrally distinct fluorophore labels for each hybridization probe, this approach gives you the power to resolve several genetic elements or multiple gene expression patterns through multicolor visual display. Life Technologies offers FISH Tag™ detection kits for routine analysis, and TSA kits for very rare or low-abundance targets.
*  Expression of Raldh1. In situ hybridisation was perform | Open-i
Expression of Raldh1. In situ hybridisation was performed on wholemount embryos with DIG- or fluorescein-labelled riboprobes. Unless otherwise stated, rostral i
*  Localization during development of alternatively spliced forms of cytotactin mRNA by in situ hybridization. | JCB
Cytotactin, an extracellular glycoprotein found in neural and nonneural tissues, influences a variety of cellular phenomena, particularly cell adhesion and cell migration. Northern and Western blot analysis and in situ hybridization were used to determine localization of alternatively spliced forms of cytotactin in neural and nonneural tissues using a probe (CT) that detected all forms of cytotactin mRNA, and one (VbVc) that detected two of the differentially spliced repeats homologous to the type III repeats of fibronectin. In the brain, the levels of mRNA and protein increased from E8 through E15 and then gradually decreased until they were barely detectable by P3. Among the three cytotactin mRNAs (7.2, 6.6, and 6.4 kb) detected in the brain, the VbVc probe hybridized only to the 7.2-kb message. In isolated cerebella, the 220-kD polypeptide and 7.2-kb mRNA were the only cytotactin species present at hatching, indicating that the 220-kD polypeptide is encoded by the 7.2-kb message that ...
*  Epi-illumination microscopy coupled to in situ hybridization and its utility in the study of evolution and development in non...
Evolutionary developmental biology often combines methods for examining morphology (e.g., scanning electron microscopy, SEM) with analyses of gene expression (e.g., RNA in situ hybridization). Due to
*  In situ hybridization of eye from bioassay. Example of | Open-i
In situ hybridization of eye from bioassay. Example of confocal photomictographs of the fasciculated zone of the eye of a challenged shrimp specimen from test 2
*  Fluorescence in situ hybridization (FISH) in Human Sperm
... It has been reported that 12% of the population has fertility problems. The male factor in infertility is proving to make up about 50% of all couples
*  Fluorescence in situ Hybridization(荧光原位杂交)
简介荧光原位杂交(fluorescence in situ hybridization,FISH)是在20世纪80年代末在放射性原位杂交技术的基础上发展起来的一种非放射性分子细胞遗传技术,以荧光标记取代同位素标记而形成的一种新的原位杂交方法。探针首先与某种介导分子(reporter molecule)结合,杂交后再通过免疫细胞化学过程连接上荧光染料。FISH的基本原理是将DNA(或RNA)探针用特
*  Database Guide: Search - Virtual Library of Biology (vifabio)
Ascertaining when and where genes are expressed is of crucial importance in order to understand the physiological role of a given gene/protein and the interactions between them. In addition, the normal expression patterns can then be compared to those observed in a variety of pathological conditions to identify pathological hallmarks of gene expression. The EURExpress, an integrated project funded by the EU under the VI Framework proposes a transcriptome-wide acquisition of expression patterns chiefly by means of in situ hybridization (ISH) with non-radioactive probes and will use this data to establish a web-linked, interactive digital transcriptome atlas of embryonic mouse. The final goal of the project is to create the expression data of > 20,000 genes by RNA in situ hybridization on sagittal sections from E14.5 wild type murine embryos. This data will result in a detailed description (at a cellular level) of gene expression patterns in the developing mouse. The ...
*  Practical in situ hybridization | Oxfam GB | Oxfam's Online Shop
Buy Practical in situ hybridization, Oxfam, Trude schwarzacher and Pat Heslop-Harrison, 9781859961384, 9781859961384, Books, Science and Nature
*  Characterization of the transporterB0AT3 (Slc6a17) in the rodent central nervous system | BMC Neuroscience | Full Text
In this study we used quantitative RT-PCR (qPCR) in a large panel of adult rat brain and peripheral tissues (Figure 1), to further refine the expression profile of the Slc6a17 gene. The highest expression levels of Slc6a17 mRNA were found in hindbrain, various brain cross sections, cerebellum, spinal cord, brain stem and hypothalamus, while very low or no expression was seen in the peripheral tissues with the exception of epididymis. Consequently, the Slc6a17 transporter is highly and selectively expressed in the CNS of adult rat.. Abundant mRNA expression of Slc6a17 in adult and embryonic rat CNS has previously been shown using in situ hybridization. Consistent results indicated restricted expression exclusively in neurons, both glutamatergic and subsets of GABAergic [4, 9, 15-17]. Our results from in situ hybridization (Figure 3) shows that the mouse Slc6a17 gene has similar expression pattern as previously seen in rat, with high expression in mouse forebrain and midbrain ...
*  imagery | HCS-pharma
We describe a fluorescence in situ hybridization method that permits detection of the localization and abundance of single mRNAs (smFISH) in cleared whole-mount adult Drosophila brains. The approach is rapid and multiplexable and does not require molecular amplification; it allows facile quantification of mRNA expression with subcellular resolution on a standard confocal microscope. We further demonstrate single-mRNA detection across the entire brain using a custom Bessel beam structured illumination microscope (BB-SIM). ...
*  Hybridization Bags
... are used for membrane incubation steps in southern, northern, and western blotting protocols for such applications as hybridizations and washes, antibody and conjugate incubations, substrate application, and film exposure. Hybridization bags are 8 X 10 inch, 4 mil thick, clear polyethylene bags. They are heat-sealable, resistant to temperatures up to at least 65 C, and do not curl when cut ...
*  Comparison between fluorescence in situ hybridization (FISH) and quantitative-fluorescent polymerase chain reaction (QF-PCR)...
UCL Discovery is UCL's open access repository, showcasing and providing access to UCL research outputs from all UCL disciplines.
*  In Situ Hybridization - Kits : Heat Pretreatment Solution Citric
Distributor of Immunohistochemistry IHC antibodies, Flow Cytometry, Molecular Biology, Staining Kits, Reagents, Detection Systems Ancillary reagents
*  Development of an easy method to test for HER2 in breast cancer using dual-color in situ hybridization. | Sigma-Aldrich
Sigma-Aldrich offers abstracts and full-text articles by [Tomoe Nakagawa, Rie Horii, Yoshinori Ito, Takuji Iwase, Futoshi Akiyama].
*  Utility of CholangioFlex and Fluorescent in Situ Hybridization in the Diagnosis of Malignant Biliary Strictures - Full Text...
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the contacts provided below. For general information, Learn About Clinical Studies. ...
*  Gene Expression Literature Detail
J:92177 Powles N, Babbs C, Ficker M, Schimmang T, Maconochie M, Identification and analysis of genes from the mouse otic vesicle and their association with developmental subprocesses through in situ hybridization. Dev Biol. 2004 Apr 1;268(1):24-38 ...
*  Additional developmental defects resulting from amphime | Open-i
Additional developmental defects resulting from amphimedine treatment. In situ hybridization was performed on control (A, C, E, G, I, K, M, O, Q) and treated (B