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*  Neisserial porins induce B lymphocytes to express costimulatory B7-2 molecules and to proliferate. | JEM
The neisserial porins are the major protein components of the outer membrane of the pathogenic Neisseria (N. meningitidis and N. gonorrhoeae). They have been shown to be able to enhance the immune response to poorly immunogenic substances (e.g., polysaccharides, peptides, glycolipids, etc.). To explore the basis of their potent adjuvant activity, the effect of the neisserial porins on T-B cell interactions and T cell costimulation was examined. Neisserial porins increased the surface expression of the costimulatory ligand B7-2 (CD86) but did not affect the expression of B7-1 (CD80). In addition, incubation with the neisserial porins increased the T lymphocyte costimulatory ability of B lymphocytes, which was inhibited by anti-B7-2 but not anti-B7-1 monoclonal antibodies. Upregulation of B7-2 on the surface of B lymphocytes may be the mechanism behind the immunopotentiating activity of neisserial porins. ...
  http://jem.rupress.org/content/183/3/1151
*  Identification of membrane spanning beta strands in bacterial porins. - Semantic Scholar
The membrane assembly of outer membrane proteins is more complex than that of transmembrane helical proteins owing to the intervention of many charged and polar residues in the membrane. Accordingly, the predictive accuracy of transmembrane beta strands is considerably lower than that of transmembrane alpha helices. In this paper we develop a set of conformational parameters for membrane spanning beta strands. We formulate an algorithm to predict the transmembrane beta strands in the family of bacterial porins based on the conformational parameters and surrounding hydrophobicities of amino acid residues. A Fortran program has been developed which takes the amino acid sequence as the input file and gives the predicted transmembrane beta strand as output. The present method predicts at an accuracy level of 82% for all the bacterial porins considered.
  https://www.semanticscholar.org/paper/Identification-of-membrane-spanning-beta-strands-i-Gromiha-Majumdar/42e66c0ca6d8a1a16551044260fd30b81f99a189
*  Activation of bacterial porin gene expression by a chimeric signal transducer in response to aspartate | Science
The Tar chemoreceptor of Escherichia coli is a membrane-bound sensory protein that facilitates bacterial chemotaxis in response to aspartate. The EnvZ molecule has a membrane topology similar to Tar and is a putative osmosensor that is required for osmoregulation of the genes for the major outer membrane porin proteins, OmpF and OmpC. The cytoplasmic signaling domain of Tar was replaced with the carboxyl portion of EnvZ, and the resulting chimeric receptor activated transcription of the ompC gene in response to aspartate. The activation of ompC by the chimeric receptor was absolutely dependent on OmpR, a transcriptional activator for ompF and ompC. ...
  http://science.sciencemag.org/content/245/4923/1246
*  Outer membrane porin D - Wikipedia
Outer membrane porin D is a protein family containing bacterial outer membrane porins which are involved in transport of cationic amino acids, peptides, antibiotics and other compounds. It was also described as having some serine protease activity. However many of these proteins are not peptidases and are classified as non-peptidase homologues as they either have been found experimentally to be without peptidase activity, or lack amino acid residues that are believed to be essential for the catalytic activity of peptidases in the S43 family. Yoshihara E, Yoneyama H, Ono T, Nakae T (June 1998). "Identification of the catalytic triad of the protein D2 protease in Pseudomonas aeruginosa". Biochem. Biophys. Res. Commun. 247 (1): 142-5. doi:10.1006/bbrc.1998.8745. PMID 9636669. This article incorporates text from the public domain Pfam and InterPro ...
  https://en.wikipedia.org/wiki/Outer_membrane_porin_D
*  Antibiotics translocation through porins: an MD study | IRIS Univ. Cagliari
Gram-negative bacteria are protected against external attack by an outer membrane. However, general diffusion porins located in this membrane allow the entry, by simple diffusion, of small molecules and, among them, antibiotics (beta-lactams and fluoroquinolones). Strains of bacteria resistant to beta-lactams have shown either a low porin density in the outer membrane, or some mutations at the level of porins affecting their internal size. Understand how antibiotics diffuse through these porins can help researcher to develop new antibiotics with an improved penetration especially when resistance becomes a serious problem for infectious disease. Recent experimental investigations pointed out how the diffusion of antibiotics through a porin is a molecular-based process: during diffusion the antibiotics interact strongly with the amino acids of the porin. Changing either the charge or the hydrophobicity of antibiotics the interaction strenght with the porin ...
  https://iris.unica.it/handle/11584/44836
*  Brunel University Research Archive: Neisseria meningitidis Lacking the Major Porins PorA and PorB Is Viable and Modulates...
The bacterial pathogen Neisseria meningitidis expresses two major outer-membrane porins. PorA expression is subject to phase-variation (high frequency, random, on-off switching) and both PorA and PorB are antigenically variable between strains. PorA expression is variable and not correlated with meningococcal colonisation or invasive disease, whereas all naturally-occurring strains express PorB suggesting strong selection for expression. We have generated N. meningitidis strains lacking expression of both major porins, demonstrating that they are dispensable for bacterial growth in vitro. The porAB mutant strain has an exponential growth rate similar to the parental strain, as do the single porA or porB mutants, but the porAB mutant strain does not reach the same cell density in stationary phase. Proteomic analysis suggests that the double mutant strain exhibits compensatory expression changes in proteins associated with cellular redox state, energy/nutrient metabolism, and ...
  http://bura.brunel.ac.uk/handle/2438/15140
*  Brunel University Research Archive: Neisseria meningitidis Lacking the Major Porins PorA and PorB Is Viable and Modulates...
The bacterial pathogen Neisseria meningitidis expresses two major outer-membrane porins. PorA expression is subject to phase-variation (high frequency, random, on-off switching) and both PorA and PorB are antigenically variable between strains. PorA expression is variable and not correlated with meningococcal colonisation or invasive disease, whereas all naturally-occurring strains express PorB suggesting strong selection for expression. We have generated N. meningitidis strains lacking expression of both major porins, demonstrating that they are dispensable for bacterial growth in vitro. The porAB mutant strain has an exponential growth rate similar to the parental strain, as do the single porA or porB mutants, but the porAB mutant strain does not reach the same cell density in stationary phase. Proteomic analysis suggests that the double mutant strain exhibits compensatory expression changes in proteins associated with cellular redox state, energy/nutrient metabolism, and ...
  http://bura.brunel.ac.uk/handle/2438/15140?mode=full
*  Biology-Online • View topic - Porin-deficient mutant
Knowing that, what sort of experiment might you design to see whether or not the porin is present? A system where the cell dies if the porin is not present (that is, the cell can't execute the porin's function) is usually very convenient ...
  http://www.biology-online.org/biology-forum/post-141263.html
*  Major outer membrane protein elisa and antibody
Shop Major outer membrane protein ELISA Kit, Recombinant Protein and Major outer membrane protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
  https://www.mybiosource.com/protein_family.php?root=major-outer-membrane-protein
*  RCSB PDB - 1GFM: OMPF PORIN (MUTANT D113G) Structure Summary Page
1GFM: Structural and functional characterization of OmpF porin mutants selected for larger pore size. I. Crystallographic analysis.
  http://www.rcsb.org/pdb/explore/explore.do?structureId=1gfm
*  RCSB PDB for 1GFN
1GFN: Structural and functional characterization of OmpF porin mutants selected for larger pore size. I. Crystallographic analysis.
  http://www.rcsb.org/pdb/explore/biologyAndChemistry.do?structureId=1GFN
*  3pox » Porin OmpF - Orientations of Proteins in Membranes (OPM) database
A - Tilt: 2° - Segments: 1( 11- 23), 2( 37- 48), 3( 57- 64), 4( 79- 89), 5( 96- 101), 6( 136- 142), 7( 150- 159), 8( 173- 182), 9( 186- 192), 10( 213- 221), 11( 226- 232), 12( 256- 263), 13( 270- 276), 14( 294- 302), 15( 309- 315), 16( 331- 338 ...
  http://opm.phar.umich.edu/protein.php?pdbid=3pox
*  Membrane protein insertion: mixing eukaryotic and prokaryotic concepts | EMBO Reports
Endosymbiotically derived organelles import most of their proteinaceous components from the cytosol. In mitochondria and plastids, two pore‐forming proteins-Tom40 and Toc75, respectively-are involved in the translocation of proteins across the outer membrane. However, the evolutionary development of the two transport channels is distinct. The mitochondrial channel Tom40 seems to share its ancestral roots with porins (Gabriel et al, 2001), whereas Toc75 belongs to the Omp85 class (Moslavac et al, 2005). Interestingly, another translocation system specialized for the insertion of β‐barrel OMPs (Fig 1B) was identified recently in the mitochondrial outer membrane (Wiedemann et al, 2003). The main component of this complex is Sam50 (Table 1; Paschen et al, 2003; Kozjak et al, 2003; Gentle et al, 2004; Humphries et al, 2005), which belongs to the Omp85 class and has the highest similarity with a subclass of Omp85 proteins previously annotated as Oma87 (Moslavac et al, 2005). The Sam50 protein is ...
  http://embor.embopress.org/content/6/11/1023
*  A Hybrid Asymptotic Expansion Scheme: an Application to Long-term Currency Options
Downloadable! This paper develops a general approximation scheme, henceforth called a hybrid asymptotic expansion scheme for valuation of multi-factor European path-independent derivatives. Specifically, we apply it to pricing long-term currency options under a market model of interest rates and a general diffusion stochastic volatility model with jumps of spot exchange rates. Our scheme is very effective for a type of models in which there exist correlations among all the factors whose dynamics are not necessarily affine nor even Markovian so long as the randomness is generated by Brownian motions. It can also handle models that include jump components under an assumption of their independence of the other random variables when the characteristic functions for the jump parts can be analytically obtained. An asymptotic expansion approach provides a closed-form approximation formula for their values, which can be calculated in a moment and thus can be used for calibration or for an explicit
  https://ideas.repec.org/p/tky/fseres/2010cf734.html
*  Nanoparticles | Profiles RNS
Anand A, LeDoyt M, Karanian C, Luthra A, Koszelak-Rosenblum M, Malkowski MG, Puthenveetil R, Vinogradova O, Radolf JD. Bipartite Topology of Treponema pallidum Repeat Proteins C/D and I: OUTER MEMBRANE INSERTION, TRIMERIZATION, AND PORIN FUNCTION REQUIRE A C-TERMINAL ß-BARREL DOMAIN. J Biol Chem. 2015 May 08; 290(19):12313-31 ...
  http://profiles.uconn.edu/display/62966
*  Oligogalacturonate-specific porin elisa and antibody
Shop Oligogalacturonate-specific porin ELISA Kit, Recombinant Protein and Oligogalacturonate-specific porin Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
  https://www.mybiosource.com/protein_family.php?root=oligogalacturonate-specific-porin
*  PORAŠČENA BEJBA MAIDS UNIFORMS SEKS ODLOMKI - MAID TUBE SEX.COM
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  http://maidtubesex.com/sl/cat_s/MGgtODEtNDY0/pora%C5%A1%C4%8Dena-bejba/
*  Hotel Porin Deals & Reviews, Makarska | LateRooms.com
Book a deal for Hotel Porin, Makarska at LateRooms ➨ Discount hotel rooms specialist. See Reviews, Pictures and Offers. ➨ Book Online or by Phone.
  https://www.laterooms.com/en/p1301/pv318624s/hotel-reservations/262287_hotel-porin-makarska.aspx
*  Dodjeljena nagrada Porin za 2004. godinu - muzika.hr
Nedjelja, 20. studenog, bila je glazbeni vrhunac, barem što se tiče nagrada, događanja u Hrvatskoj u 2005.-oj godine. Jučer je u osječkoj dvorani Zrinjevac, održana 12. dodjela domaće diskografske nagrade Porin, na kojoj su trijumfirali The Beat Fleet s pet osvojenih nagrada, a slijedi ih Arsen Dedić s tri Porina.. Dobitnici u najbitnijim kategorijama: ...
  http://www.muzika.hr/dodjeljena-nagrada-porin-za-2004-godinu/
*  3wi5 » Porin B (PorB), different strain, conformation 2 - Orientations of Proteins in Membranes (OPM) database
A - Tilt: 2° - Segments: 1( 4- 15), 2( 33- 47), 3( 55- 63), 4( 77- 82), 5( 89- 94), 6( 125- 136), 7( 139- 145), 8( 158- 166), 9( 170- 177), 10( 195- 204), 11( 208- 215), 12( 232- 241), 13( 244- 252), 14( 269- 277), 15( 284- 290), 16( 304- 311 ...
  http://opm.phar.umich.edu/protein.php?pdbid=3wi5
*  3a2s » Porin B (PorB) - Orientations of Proteins in Membranes (OPM) database
A - Tilt: 3° - Segments: 1(2-14), 2(34-47), 3(57-64), 4(78-84), 5(88-94), 6(132-141), 7(144-152), 8(171-179), 9(183-190), 10(219-227), 11(231-238), 12(260-268), 13(271-278), 14(297-304), 15(310-318), 16(332-340 ...
  http://opm.phar.umich.edu/protein.php?pdbid=3a2s
*  Oboz Dash - Trailspace.com
The Dash's sock-like bootie design, which keeps out debris, is combined with arch-lasted construction for a supportive fit. Lacing is asymmetrical with offset eyelets that help to reinforce the support.
  http://www.trailspace.com/gear/oboz/dash/