*  Microbiology Society Journals | A link between translation of the hepatitis C virus polyprotein and polymerase function;...
Hyperphosphorylation of NS5A is thought to play a key role in controlling hepatitis C virus (HCV) RNA replication. Using a tetracycline-regulable baculovirus delivery system to introduce non-culture-adapted HCV replicons into HepG2 cells, we found that a point mutation in the active site of the viral polymerase, NS5B, led to an increase in NS5A hyperphosphorylation. Although replicon transcripts lacking elements downstream of NS5A also had altered NS5A hyperphosphorylation, this did not explain the changes resulting from polymerase inactivation. Instead, two additional findings may be related to the link between polymerase activity and NS5A hyperphosphorylation. Firstly, we found that disabling polymerase activity, either by targeted mutation of the polymerase active site or by use of a synthetic inhibitor, stimulated translation from the replicon transcript. Secondly, when the rate of translation of non-structural proteins from replicon transcripts was reduced by use of a defective encephalomyocarditis
*  rep - Replicase polyprotein 1ab - Simian hemorrhagic fever virus (SHFV) - rep gene & protein
The replicase polyprotein 1ab is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.
*  Involvement of PKR and RNase L in translational control and induction of apoptosis after Hepatitis C polyprotein expression...
Hepatitis C virus (HCV) infection is of growing concern in public health with around 350 million chronically infected individuals worldwide. Although the IFN-α/rivabirin is the only approved therapy with 10-30% clinical efficacy, the protective molecular mechanism involved during the treatment is still unknown. To analyze the effect of HCV polyprotein expression on the antiviral response of the host, we developed a novel vaccinia virus (VV)-based delivery system (VT7-HCV7.9) where structural and nonstructural (except part of NS5B) proteins of HCV ORF from genotype 1b are efficiently expressed and produced, and timely regulated in mammalian cell lines. Regulated transcript production and viral polypeptide processing was demonstrated in various cell lines infected with the recombinant VT7-HCV7.9, indicating that the cellular and viral proteolytic machineries are functional within these cells. The inducible expression of the HCV polyprotein by VV inhibits the synthesis of both host and viral proteins over
*  E165.María Luisa Salas - ENG
The African swine fever virus (ASFV) protease that processes the viral polyproteins is required for a late maturational step in virus core assembly and production of infectious progeny virus. On the other hand, studies on ASFV morphogenesis have shown a correlation between proteolytic processing of polyproteins and correct virus assembly, suggesting that the activity of the protease might be modulated during the infection. Indeed, recent studies demonstrated that the protease activity is controlled by two disulfide bridges formed between cysteines C14 and C24 and between C45 and C50. To investigate the mechanism of this redox regulation, we have studied the interaction of the wild type protease and punctual mutants of the involved cysteines to serine with the substrate polyprotein pp62. Our results show, in pull down experiments, the binding of the wild type but not the mutated protease to polyprotein pp62, indicating that the intramolecular disulfide bonds are necessary for ...
*  PRCII, a representative of a new class of avian sarcoma viruses - Enlighten: Publications
The Poultry Research Center Virus II (PRC II) is a replication-defective avian sarcoma virus with envelope determinants of the A and B subgroups. In nonproducing cells transformed by PRCII the products of the replicative genes gag, pol, and env are not demonstrable, but a single polyprotein of Mr 105,000 (p105) can be detected. P105 contains peptides of the gag proteins p19 and p27 plus transformationspecific sequences. It does not contain peptides of gPr95env of Pr180gag-PoI (with the possible exception of one pol peptide). The transformation-specific sequences of p105 are distinct form those of p100 of avian carcinoma virus MH2, of p1 10 coded for by avian myelocytoma virus MC29, and of p75 or p40 of avian erythroblastosis virus AEV. They also show no resemblance to p60src of Rous sarcoma virus. P105 is phosphorylated on a tyrosine residue and has an associated Phosphokinase activity. P105 appears to be capable of autophosphorylation and of phosphorylating homologous immunoglobulin. ...
*  HCV Research and News: August 2010
The HCV replication complex. After clathrin-mediated endocytosis, fusion of HCV with cellular membranes, and uncoating the viral nucleocapsid, the single-stranded positive-sense RNA genome of the virus of approximately 9600 nucleotides is released into the cytoplasm to serve as a messenger RNA for the HCV polyprotein precursor. The HCV genome contains a single large open reading frame encoding for a polyprotein of approximately 3100 amino acids. The translated section of the HCV genome is flanked by the strongly conserved HCV 3′ and 5′ untranslated regions (UTR). The 5′ UTR is comprised of four highly structured domains forming the internal ribosome entry site (IRES), which is a virus-specific structure to initiate HCV mRNA translation. From the initially translated polyprotein, the structural HCV protein core (C) and envelope 1 and 2 (E1, E2); p7; and the six nonstructural HCV proteins NS2, NS3, NS4A, NS4B, NS5A and NS5B, are processed by both viral and host proteases. The core protein ...
*  Structure and Mechanisms of a Protein-Based Organelle in Escherichia coli | Science
Many bacterial cells contain proteinaceous microcompartments that act as simple organelles by sequestering specific metabolic processes involving volatile or toxic metabolites. Here we report the three-dimensional (3D) crystal structures, with resolutions between 1.65 and 2.5 angstroms, of the four homologous proteins (EutS, EutL, EutK, and EutM) that are thought to be the major shell constituents of a functionally complex ethanolamine utilization (Eut) microcompartment. The Eut microcompartment is used to sequester the metabolism of ethanolamine in bacteria such as Escherichia coli and Salmonella enterica. The four Eut shell proteins share an overall similar 3D fold, but they have distinguishing structural features that help explain the specific roles they play in the microcompartment. For example, EutL undergoes a conformational change that is probably involved in gating molecular transport through shell protein pores, whereas structural evidence suggests that EutK might bind a nucleic acid ...
*  Ethanolamine utilization protein EutL (P0A1D0) | InterPro | EMBL-EBI
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
*  DCV capsid polyprotein抗体|Abcam中国|Anti-DCV capsid polyprotein抗体
DCV capsid polyprotein兔多克隆抗体(ab92954)可与重组片段样本反应并经WB, ELISA实验严格验证并得到1个独立的用户反馈。所有产品均提供质保服务,中国75%以上现货。
*  Probable antibacterial peptide polyprotein elisa and antibody
Shop Probable antibacterial peptide polyprotein ELISA Kit, Recombinant Protein and Probable antibacterial peptide polyprotein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
*  Transposon Tf2-8 polyprotein elisa and antibody
Shop Transposon Tf2-8 polyprotein ELISA Kit, Recombinant Protein and Transposon Tf2-8 polyprotein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
*  Transposon Ty2-OR2 Gag polyprotein elisa and antibody
Shop Transposon Ty2-OR2 Gag polyprotein ELISA Kit, Recombinant Protein and Transposon Ty2-OR2 Gag polyprotein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
*  January | 2017 | HSP signaling
Samples were collected at the time points indicated in Table 4. The dogs received no additional protection or treatment either in the clinic or in the care of their owners other than standard clinical care and immunizations. In the event the evaluating veterinarian determined a dog was getting sicker due to CVL, the dog was given buy GSK126 rescue treatment with chemotherapy and continued in follow up. The last CS before death or rescue treatment was used for calculating a mean CS for the treatment group in the remaining time points through Day 180. Peripheral blood samples were. collected from a radial vein at Day 0 and one week after the last vaccination (either Day 30 or Day 42) for plasma isolation. Those plasma samples were used for antibody ELISA to examine responses of dogs to Leish-111f, the vaccine antigen. For these analyses Leish-111f was diluted in sodium carbonate buffer, pH 9.6, and used. to coat Nunc 96-well Polysorp plates (Thermo Fisher Scientific Inc., Waltham, MA), as ...
*  Lens :: A New Way of Looking at Science || Secrets of a deadly virus: seven potential ways to stop HIV
After transport to the cytoplasm, some of the RNA is translated - using the cell's protein-making machinery - into large polyproteins. Other cellular proteins transport the polyproteins to the cell membrane, and help construct a new virion. One of these proteins, called Tsg101, seems to be important in budding. "If you reduce the levels of Tsg101 in the cell, you see a lot of virions on the surface of the cell. They're trying to pinch off but they can't quite release," says Chris Aiken, Ph.D., associate professor of Microbiology and Immunology at Vanderbilt.. ...
*  Gentaur Molecular :bio-gentaur \ VIROLOGY Parechovirus Type 5 Culture Fluid \ GEN0810149CF
Gentaur molecular products has all kinds of products like :search , bio-gentaur \ VIROLOGY Parechovirus Type 5 Culture Fluid \ GEN0810149CF for more molecular products just contact us
*  Epitopes described in Differential immune responses and protective efficacy induced by components of a tuberculosis polyprotein...
Free resource for searching and exporting immune epitopes. Includes more than 95% of all published infectious disease, allergy, autoimmune, and transplant epitope data.
*  ENK11 HUMAN » HERV-K 22q11.21 provirus ancestral Env polyprotein - Membranome database
*  ERVV1 HUMAN » HERV-V 19q13.41 provirus ancestral Env polyprotein 1 - Membranome database
*  HOXD1 / HOX4G antibody | acris-antibodies.com
HOXD1 is a protein with a homeobox DNA-binding domain, and it belongs to the Antp homeobox family. This nuclear protein functions as a…
*  Mapping of the Self-Interaction Domains in the Simian Immunodeficiency Virus Gag Polyprotein
To gain a better understanding of the assembly process in simian immunodeficiency virus (SIV), we first established the conditions under which recombinant SIV Gag lacking the C-terminal p6 domain (SIV GagΔp6) assembled in vitro into spherical particles. Based on the full multimerization capacity of SIV GagΔp6, and to identify the Gag sequences involved in homotypic interactions, we next developed a pull-down assay in which a panel of histidine-tagged SIV Gag truncation mutants was tested for its ability to associate in vitro with GST-SIVGagΔp6. Removal of the nucleocapsid (NC) domain from Gag impaired its ability to interact with GST-SIVGagΔp6. However, this Gag mutant consisting of the matrix (MA) and capsid (CA) domains still retained 50% of the wild-type binding activity. Truncation of SIV Gag from its N-terminus yielded markedly different results. The Gag region consisting of the CA and NC was significantly more efficient than wild-type Gag at interacting in vitrowith GST-SIVGagΔp6. ...
*  SGDB | Show Synthetic Gene ID: 158
Codon-optimized genes were synthesized for the SIVmac239 Gag, a mutant Gag with mutations in the major homology region, and a chimeric Gag containing a protein destruction signal at the N-terminus of Gag. The mutant and chimeric Gag were expressed at levels comparable to that observed for the wild-type Gag protein but their stability and release into the medium were found to be significantly reduced. Immunization of mice with DNA vectors encoding the mutant or chimeric Gag induced fourfold higher levels of anti-SIV Gag CD4 T cell responses than the DNA vector encoding the wild-type SIV Gag. Moreover, anti-SIV Gag CD8 T cell responses induced by DNA vectors encoding the mutant or chimeric Gag were found to be 5- to 10-fold higher than those induced by the DNA construct for the wild-type Gag. These results indicate that mutations disrupting assembly and/or stability of the SIV Gag protein effectively enhance its immunogenicity when expressed from DNA vaccines ...
*  Human Immunodeficiency Virus gag and protease: partners in resistance | Retrovirology | Full Text
Detailed structural knowledge of HIV PR and its substrate led to the development of specific protease inhibitors (PIs). To date, nine different PIs have been approved for clinical use: saquinavir (SQV), ritonavir (RTV), indinavir (IDV), nelfinavir (NFV), (fos)amprenavir (FPV/APV), lopinavir (LPV), atazanavir (ATV), tipranavir (TPV) and darunavir (DRV). All PIs, with the exception of tipranavir, are competitive peptidomimetic inhibitors, mimicking the natural substrate of the viral PR. The peptidomimetic inhibitors contain a hydroxyethylene core which prohibits cleavage by the viral PR [18-25]. Instead of a hydroxyethylene core, tipranavir contains a dihydropyrone ring as a central scaffold [26]. In general, all these compounds have been designed to bind to the substrate binding region of the mature viral PR dimer with high affinity, but they tend to occupy more space than the natural substrate. For tipranavir and darunavir, it has been demonstrated that they have a dual mechanism of inhibition ...
*  Chimeric retroviral gag genes and screening assays - Drawing for Patent # 7148325 - PatentGenius
The subject invention provides novel and advantageous methods for identifying amino acid sequences in random peptide libraries that can bind to Gag polypeptides. The subject invention also establishes a novel in vitro system that can be used to test competitive inhibitors of retrovrial capsid assembly. Also provided are peptides, and compositions containing these peptides, which are inhibitors of the retrovirus Gag protein(s) function. Chimeric Gag polypeptides are also provided.
*  Intranasal Modified Vacc-4x Gag Peptides With Endocine as Adjuvant - Full Text View - ClinicalTrials.gov
HIV-specific cellular immunity is hampered in most HIV-infected individuals, partly because the virus infects CD4+ T cells, the key cell subset in all immune responses. CD4 is the primary HIV receptor (CD4), but infection requires a co-receptor (CCR5) which is carried mainly by activated T cells. During primary HIV-infection, two types of CD4+ T cells mainly become infected: (i) Sub-activated T cells of all specificities within the mucosal linings, particularly in the gut; and (ii) HIV-specific T cell clones, that proliferates and are activated as a normal response to HIV infection itself. The HIV-specific immunity therefore becomes severely compromised early in the infection. Patients having better T cells specific to parts of the HIV Gag matrix protein usually progress slower towards AIDS than patients with poor T cell responsitivity towards Gag.. Therapeutic immunization in HIV aims to strengthen the HIV-specific cellular immunity, usually in the absence of replicating HIV with antiretroviral ...
*  HIV Gag Precursor Protein Interactions - Eric Barklis
Despite great advances in AIDS diagnosis and treatment, the continuing devastation of the AIDS epidemic demands continuing efforts to understand all aspects of...