Characterisation of Low Level Heteroplasmic Mitochondrial Mutations by single Stranded Conformational Polymorphism (SSCP) - Pcr...
Use of single-strand conformational polymorphism method to detect recurrent and novel mutations in the low - density...
Single-strand conformational polymorphism analysis: basic principles and routine practice. - Fingerprint - Augusta...
Identification of two novel mutations in the ventricular regulatory myosin light chain gene (MYL2) associated with familial and...
Polymorphism, Restriction Fragment Length * Polymorphism, Single-Stranded Conformational * Sequence Alignment Substances * ... Single-strand conformation polymorphism analysis was used to search for mutations in the coding segments of the MYL2 gene, and ...
BRCA1, BRCA2, and hereditary nonpolyposis colorectal cancer gene mutations in an unselected ovarian cancer population:...
Polymorphism, Single-Stranded Conformational * Sequence Analysis, DNA * Transcription Factors / genetics* Substances * BRCA2 ... single-strand conformation polymorphism analysis, and direct gene sequencing. Fishers exact test was used to evaluate possible ... and a single BRCA2 mutation, which occurred in a patient also carrying a BRCA1 mutation. More than half the patients with BRCA1 ...
Wildlife forensic science - Wikipedia
Dong, Yanbin; Zhu, Haidong (2005). Single-strand conformational polymorphism analysis: basic principles and routine practice. ... single-stranded) to maximize self-annealing detection of mobility difference of the single-stranded DNAs by electrophoresis ... species that are candidates for genetic rescue using things such as the SSCP or Single-Strand Conformational Polymorphism gel ... Any altered conformation due to a single base change in the sequence can cause single-stranded DNA to migrate differently under ...
CASP5 | Cancer Genetics Web
Single-Stranded Conformational Polymorphism. Tag cloud generated 31 August, 2019 using data from PubMed, MeSH and CancerIndex ... and 60 lung carcinomas by a single-strand conformation polymorphism assay. We detected 2 (0.6%) caspase-1, 2 (0.6%) caspase-4, ... Sixty-three tagging single nucleotide polymorphisms (tSNPs) in these genes were genotyped in 1,799 invasive ovarian cancer ... To test our proposal we examined whether six single nucleotide polymorphisms (SNPs) of the DR4 and Casp3, 5 genes contrive the ...
Biblio | Page 9 | Linus Pauling Institute | Oregon State University
Polymorphism, Single-Stranded Conformational. Li Q, Dixon BM, Al-Fageeh M, Blum CA, Dashwood RH. 2002. Sequencing of the rat ... Polymorphism, Single Nucleotide. Blum CA, Tanaka T, Zhong X, Li Q, Dashwood W-M, Pereira C, Xu M, Dashwood RH. 2003. Mutational ... Polymorphism, Genetic. Higdon JV, Delage B, Williams DE, Dashwood RH. 2007. Cruciferous vegetables and human cancer risk: ...
Biblio | Linus Pauling Institute | Oregon State University
Int7G24A Variant of Transforming Growth Factor-β Receptor Type I Is Associated with Invasive Breast Cancer | Clinical Cancer...
PCR and single-strand conformational polymorphism. A total of 71 breast cancers were first screened for somatic mutations in ... PCR-single-strand conformational polymorphism (SSCP; ref. 27). Primer sequences for PCR amplification of the nine exons of the ... and somatic mutations in the TGFBR1 were also analyzed by PCR and single-strand conformational polymorphism. ... "Cold SSCP": a simple, rapid and non-radioactive method for optimized single-strand conformation polymorphism analyses. Nucleic ...
Publications at this Location : USDA ARS
USE OF CAPILLARY ARRAY ELECTROPHORESIS SINGLE STRAND CONFORMATIONAL POLYMORPHISM ANALYSIS TO ESTIMATE GENETIC DIVERSITY OF ... Single strand conformation polymorphism analysis of candidate genes for reliable identification of alleles by capillary array ...
Dystrophinopathies Workup: Laboratory Studies, Imaging Studies, Other Tests
Atp5f1b MGI Mouse Gene Detail - MGI:107801 - ATP synthase F1 subunit beta
A novel mutation in the von Hippel-Lindau gene.
Polymorphism, Single-Stranded Conformational. *Polymerase Chain Reaction. *Point Mutation. *Molecular Sequence Data ...
Frequency of breast cancer attributable to BRCA1 in a population-based series of American women.
Polymorphism, Single-Stranded Conformational. *Polymerase Chain Reaction. *Pedigree. *Ovarian Neoplasms. *North Carolina ... Screening was performed using multiplex single-strand conformation analysis, with all potential variants confirmed using ... An additional 5 patients had rare missense mutations or a single amino acid deletion, the biological significance of which is ...
Publications - Dr Bonnie Webster
Data are reported on the use of single stranded conformational polymorphism (SSCP) analysis of the nuclear ribosomal second ... Single-strand conformation polymorphism (SSCP) analysis of the second internal transcribed spacer (ITS2) of nuclear ribosomal ... Webster BL, Tchuem Tchuenté LA, Southgate VR, 2007, A single-strand conformation polymorphism (SSCP) approach for investigating ... A single forward primer and two species-specific reverse primers were used to produce a polymerase chain reaction (PCR) ...
Molecular Changes in Second Primary Lung and Breast Cancers after Therapy for Hodgkin's Disease1 | Cancer Epidemiology,...
... in exons 5-8 of the TP53 gene by single-strand conformational polymorphism analysis followed by sequencing of both strands of ... single-strand conformational polymorphism analysis, and primer sequences used have been described previously (22). For K-ras ... Strand M., Prolla T. A., Liskay R. M., Petes T. D. Destabilization of tracts of simple repetitive DNA in yeast by mutations ... Weber J. L., May P. E. Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. Am. J ...
Elizabeth Egan | Stanford Medicine
... we have genetically mapped 104 genes and expressed sequence tags by scoring single-strand conformational polymorphisms in a ... we have genetically mapped 104 genes and expressed sequence tags by scoring single-strand conformational polymorphisms in a ... we have genetically mapped 104 genes and expressed sequence tags by scoring single-strand conformational polymorphisms in a ... "A Common Polymorphism in the Mechanosensitive Ion Channel PIEZO1 Is Associated with Protection from Severe Malaria in Humans ...
Genetic Variation of the GLUT10 Glucose Transporter (SLC2A10) and Relationships to Type 2 Diabetes and Intermediary Traits |...
The IVS4 + 18T→G variant was genotyped using single-strand conformational polymorphism with conditions as described (Table 3). ... for combined single-strand conformational polymorphism and heteroduplex analysis performed as described elsewhere (16). PCR ... there was no evidence of linkage between these single nucleotide polymorphisms (R2 values between 0 and 0.03). The distance ... mutation analysis of the coding region of the SLC2A10 gene and the identification of six novel single nucleotide polymorphisms ...
Medical Science Monitor | Evidence from 40 Studies that 2 Common Single-Nucleotide Polymorphisms (SNPs) of RNASEL Gene Affect...
To explore the role of RNASEL gene polymorphisms in prostate cancer, we carried out the present meta-analysis. MATERIAL AND ... Numerous studies have evaluated the relationship between RNASEL gene polymorphisms (rs486907 G,A and rs627928 T,G) and the risk ... Keywords: Anus Neoplasms, Polymorphism, Single-Stranded Conformational, Ribonuclease, Pancreatic, Alleles, Asians, Case-Control ... Evidence from 40 Studies that 2 Common Single-Nucleotide Polymorphisms (SNPs) of RNASEL Gene Affect Prostate Cancer ...
Novel variants in the hepatocyte nuclear factor-1-alpha gene in MODY and early onset NIDDM: Evidence for a mutational hotspot...
SequaGel MD - National Diagnostics
... single-strand conformational polymorphism) and heteroduplex analysis.. DNA mutations-or sequence modifications-are readily ... Since a variation of a single nucleotide in a sequence may indicate a significant genetic anomaly, an extremely sensitive ... Two principal methods of conformational analysis have been developed, SSCP Analysis and heteroduplex analysis. SequaGel MD is ...
Absence of germline p16 INK4a alterations in p53 wild type Li-Fraumeni syndrome families | Journal of Medical Genetics
... all been previously screened for the presence ofp53 mutations in exons 2 and 4-11 by single strand conformational polymorphism ... 1991) Detection of p53 gene mutations in human brain tumors by single-strand conformation polymorphism analysis of polymerase ... one subject with a polymorphism (Arg213Arg) and a 1 bp deletion, and one subject with a polymorphism (Arg72Pro) and a 2 bp ... Only one sample showed a p53 mutation along with thep16INK4a exon 2 polymorphism. There was no evidence forp16INK4a deletion in ...
p53 mutations and protein expression in primary cultures of normal oral mucosa in smokers and non-smokers - McMaster Experts
Polymorphism, Single-Stranded Conformational (MeSH) * Smoking (MeSH) * Tumor Suppressor Protein p53 (MeSH) ...
An investigation of the role of interleukin-6 in systemic-onset juvenile chronic arthritis - UCL...
Single-strand conformational polymorphism analysis of a PCR-generated fragment from the 5 flanking region of the IL-6 gene (- ... Sequencing of this region from 57 individuals revealed two novel polymorphisms; a G/C polymorphism at position -174; and a ... This thesis discusses research conducted to examine the hypothesis that this is due to polymorphisms in the region of the IL-6 ... RFLP analysis of the -174 polymorphism in 59 Caucasian S-JCA patients demonstrated that they had a lower C allele frequency ...
JCI Insight - Single-cell analysis of glandular T cell receptors in Sjögren's syndrome
... single-strand conformational polymorphism analysis (17, 18), or PCR in combination with hybridization techniques (10, 19, 20). ... Single-cell RT-PCR. The single-cell RT-PCR protocol for TCRs was adapted from Wang et al. (63) with minor modifications. In the ... Single-cell TCR sequence and repertoire analysis. TCR nucleotide sequences from single-cell RT-PCR were deposited in GenBank ( ... from SG single-cell suspensions varied considerably among participants (Supplemental Table 1). A maximum of 378 viable single ...
A robust, low- to medium-throughput prnpgenotyping system in sheep | BMC Infectious Diseases | Full Text
By optimised primer design it was possible to type the 4 relevant single nucleotide polymorphisms (SNPs) in the prnp ... Therefore, the demand on genotyping capacities of the polymorphisms of the prion protein gene (prnp) relevant to presently ... PCR-single-strand conformational polymorphism (PCR-SSCP) [8], hybridisation with allele-specific oligonucleotides [9, 10], ... By optimised primer design it was possible to type the 4 relevant single nucleotide polymorphisms (SNPs) in the prnp ...
Newcastle Disease Virus Detection from Chicken Organ Samples Using Reverse Transcriptase Polymerase Chain Reaction |...
Chondrosarcoma | Cancer Genetics Web
Single-Stranded Conformational Polymorphism. *EWSR1. *Bone Cancer. *Karyotyping. *p53 Protein. *Polymerase Chain Reaction ... Genomic analysis of single nucleotide polymorphisms showed aberrant genomic contents. The DNA sequencing revealed the absence ... Comparative analysis of exomes from two third-degree affected relatives led us to identify a single common disruptive variation ...