One-channel Cell-attached Patch-clamp Recording | Protocol
... here is a procedure for obtaining long stretches of current recording from one ion channel with the cell-attached patch-clamp ... using the patch-clamp technique. Patch-clamp electrophysiology is the first and most widely used experimental method ... Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch ... In summary, the patch-clamp technique, through its unprecedented capacity to resolve signals from one molecule remains, to date ...https://www.jove.com/video/51629/one-channel-cell-attached-patch-clamp-recording
Patch Clamp Techniques - From Beginning to Advanced Protocols | Yasunobu Okada | Springer
... and widely used patch clamp (PC) techniques. Practical descriptions of PC techniques for beginners and ... slice patch, blind patch, in vivo patch, imaging patch, smart patch, and automated patch clamping. These patch clamp techniques ... manual and text on Patch clamp techniques. The whole cell, inside out patches, and membrane techniques are well illustrated. … ... Patch Clamp Techniques. Book Subtitle. From Beginning to Advanced Protocols. Editors. * Yasunobu Okada ...http://www.springer.com/us/book/9784431539926
New illumination technique for IR-video guided patch-clamp recording from neurons in slice cultures on biomembrane - Alix...
With this technique, we demonstrate visually guided whole-cell patch-clamp recording of Na - and K -currents as well as ... New illumination technique for IR-video guided patch-clamp recording from neurons in slice cultures on biomembrane. ... Reference : New illumination technique for IR-video guided patch-clamp recording from neurons in .... ... en] Patch-clamp ; Hippocampus ; Biomembrane. Abstract :. [en] Slice cultures on biomembrane are the method of choice for ...http://orbi.ulg.ac.be/handle/2268/125782
Electroporation of plant cells studied with the patch clamp technique
... Wegner, L.H.; Schönwälder, S. ...https://publikationen.bibliothek.kit.edu/240088571
Exploring Arterial Smooth Muscle Kv7 Potassium Channel Function using Patch Clamp Electrophysiology and Pressure Myography |...
Patch Clamp Technique. Kaneez, F. S. Intech. (2012).. *Berra-Romani, R. TTX-sensitive voltage-gated Na+ channels are expressed ... Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes… ... Patch Clamp Araştırmaları jove_title ', 3. Hücre İzolasyonu * İzolasyon Çözüm 0,5 L (Tablo 1) hazırlayın ve pH ayarlamadan önce ... Patch Clamp Elektrofizyoloji ve Basınç kasılmaların kâğıda çizilmesi kullanarak arteriyel düz kas KV7 Potasyum Kanal Fonksiyon ...https://www.jove.com/video/4263/patch-clamp-elektrofizyoloji-ve-basn-kaslmalarn-kda-izilmesi?language=Turkish
Frontiers | Dendritic patch-clamp recordings from cerebellar granule cells demonstrate electrotonic compactness | Frontiers in...
Here, we performed patch-clamp recordings from GC dendrites in acute brain slices of mice to directly analyze the electrotonic ... Here, we performed patch-clamp recordings from GC dendrites in acute brain slices of mice to directly analyze the electrotonic ... patch-clamp techniques, electrophysiology. Citation: Delvendahl I, Straub I and Hallermann S (2015) Dendritic patch-clamp ... Here, we performed patch-clamp recordings from GC dendrites in acute brain slices of mice to directly analyze the electrotonic ...https://www.frontiersin.org/articles/10.3389/fncel.2015.00093/full
Modulation of Calcium Current in Arteriolar Smooth Muscle by αvβ3 and α5β1 Integrin Ligands | JCB
Patch Clamp Techniques. Perforated, whole-cell recordings were made as described previously (Rae and Fernandez, 1991). ... In B, a red blood cell can be seen on the chamber bottom (underneath, not inside, the patch pipette). The pipette at the bottom ... Both panels: bath solution is 20 Ba2+; patch pipette solution is high Cs+; second pipette solution: 20 Ba2+. Bar, 10 μm. ... 1991) Perforated patch recordings in physiology. NIPS (News Physiol Sci) 6:273-277. ...http://jcb.rupress.org/content/143/1/241
KAKEN - Research Projects | In vivo patch-clamp analysis of the thermal response in VR-1 knockout mouse (KAKENHI-PROJECT...
Journal Article] Application of in vivo patch-clamp technique to pharmacological analysis of synaptic transmission in the CNS. ... Journal Article] Application of in vivo patch-clamp technique to pharmacological analysis of synaptic transmission in the CNS. ... Journal Article] In-vivo patch-clamp analysis of synaptic responses in rat primary somatosensory barrel cortex (SI).2004. *. ... To clarify the function of the receptor in in vivo condition, we need an in vivo patch-clamp recording method which enables us ...https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15300135/
ZARM: PATCH - CLAMP
Mainly electrophysiological methods, such as bilayer and patch-clamp techniques were used. Additionally, the reaction of self ... Meissner, K. and Hanke W., Patch-Clamp experiments under micro gravity, J. Gravit. Physiol. 9, P377-8 (2002) ... gravitational impact on ion channels incorporated into planar lipid bilayers (PATCH-CLAMP / streulicht). ... we used the well established model of the retinal spreading depression with mainly optical methods and video imaging techniques ...https://www.zarm.uni-bremen.de/en/drop-tower/projects/biology/patch-clamp.html
Neutrophil Secretion Induced by an Intracellular Ca Rise and Followed by Whole-Cell Patch-Clamp Recordings Occurs Without any...
We have investigated calcium-induced secretion in human neutrophils, using a whole-cell patch-clamp technique. Mobilization of ... Rise and Followed by Whole-Cell Patch-Clamp Recordings Occurs Without any Selective Mobilization of Different Granule ...https://www.hindawi.com/journals/bmri/2006/097803/abs/
'action potentials' Protocols and Video...
Slice Patch Clamp Technique for Analyzing Learning-Induced Plasticity', 'Membrane Potentials, Synaptic Responses, Neuronal ... Whole-cell Patch-clamp Recordings in Brain Slices', 'Examination of Synaptic Vesicle Recycling Using FM Dyes During Evoked, ... Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis', 'An In-vitro Preparation of ... Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells', 'Local Field Fluorescence Microscopy: Imaging Cellular Signals ...https://www.jove.com/keyword/action+potentials
'membrane potentials' Protocols and Video...
The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry', 'Whole-cell Patch-clamp Recordings for ... Slice Patch Clamp Technique for Analyzing Learning-Induced Plasticity', 'Imaging Membrane Potential with Two Types of ... Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells', 'Modeling Neural Immune Signaling of Episodic and Chronic ... Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons ...https://www.jove.com/keyword/membrane+potentials?page=1
'cytochromes a' Protocols and Video...
Slice Patch Clamp Technique for Analyzing Learning-Induced Plasticity', 'Differential Effects of Lipid-lowering Drugs in ... Advanced Confocal Microscopy Techniques to Study Protein-protein Interactions and Kinetics at DNA Lesions', 'In Vivo Imaging of ... Slice Patch Clamp Technique for Analyzing Learning-Induced Plasticity. Hiroyuki Kida1, Yuya Sakimoto1, Dai Mitsushima1 ... Advanced Confocal Microscopy Techniques to Study Protein-protein Interactions and Kinetics at DNA Lesions. Soňa Legartová*1, ...https://www.jove.com/keyword/cytochromes+a
'gabaergic neurons' Protocols and Video...
Slice Patch Clamp Technique for Analyzing Learning-Induced Plasticity', 'Lineage-reprogramming of Pericyte-derived Cells of the ... Whole-cell Patch-clamp Recordings in Brain Slices', 'Live Imaging of Nicotine Induced Calcium Signaling and Neurotransmitter ... Patch-clamp Capacitance Measurements and Ca2+ Imaging at Single Nerve Terminals in Retinal Slices', 'Ratiometric Calcium ... Whole-cell Patch-clamp Recordings from Morphologically- and Neurochemically-identified Hippocampal Interneurons', 'Combined ...https://www.jove.com/keyword/gabaergic+neurons
Establishment of β-Adrenergic Modulation of L-Type Ca2+ Current in the Early Stages of Cardiomyocyte Development | Circulation...
Stable Recording of ICaL by Whole-Cell Patch-Clamp Technique. The whole-cell configuration of the patch-clamp technique23 was ... Improved patch-clamp techniques for high-resolution current recording from cells and cell free membrane patches. Pflugers Arch. ... ICaL was measured under voltage-clamp conditions by L/M EPC-7 patch-clamp amplifier (List Electronic) or an Axopatch 200A (Axon ... different developmental stages in murine embryonic stem cell-derived cardiomyocytes using the whole-cell patch-clamp technique ...http://circres.ahajournals.org/content/84/2/136
'vitelline membrane' Protocols and Video...
Patch Clamp Recording of Ion Channels Expressed in Xenopus Oocytes', 'A Method of Permeabilization of Drosophila Embryos for ... Technique to Target Microinjection to the Developing Xenopus Kidney', 'Identification Of Erythromyeloid Progenitors And Their ... Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes', 'Xenopus Oocytes: Optimized ... Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes. Junqiu Yang1, Kelli Delaloye2, ...https://www.jove.com/keyword/vitelline+membrane
Super-Resolution Scanning Patch-Clamp Reveals Clustering of Functional Ion Channels in the Adult Ventricular Myocyte |...
Existing methods like immunofluorescence and patch-clamp techniques are limited in their ability to localize functional ion ... Super-Resolution Scanning Patch-Clamp Reveals Clustering of Functional Ion Channels in the Adult Ventricular Myocyte. Anamika ... Super-Resolution Scanning Patch-Clamp Reveals Clustering of Functional Ion Channels in the Adult Ventricular Myocyte ... Super-Resolution Scanning Patch-Clamp Reveals Clustering of Functional Ion Channels in the Adult Ventricular Myocyte ...http://circres.ahajournals.org/content/early/2013/02/28/CIRCRESAHA.111.300445
VLDL-triglyceride Kinetics in Type 2 Diabetes
Patch-clamp Techniques. An electrophysiologic technique for studying cells, cell membranes, and occasionally isolated ... The four most common variants include on-cell patch, inside-out patch, outside-out patch, and whole-cell clamp. Patch-clamp ... All patch-clamp methods rely on a very high-resistance seal between a micropipette and a membrane; the seal is usually attained ... Glucose Clamp Technique. Maintenance of a constant blood glucose level by perfusion or infusion with glucose or insulin. It is ...https://www.bioportfolio.com/resources/trial/69570/VLDL-triglyceride-Kinetics-in-Type-2-Diabetes.html
Differential ion accumulation and ion fluxes in the mesophyll and epidermis of barley.
Patch-Clamp Techniques. Phosphates / metabolism. Plant Leaves / metabolism. Potassium / metabolism. Protoplasts / metabolism. ... However, patch-clamp data suggested that differential regulation of instantaneously activating ion channels in the plasma ... by microelectrode impalement and patch clamping) and radiometrically. Data from both approaches suggested that similar types of ...http://www.biomedsearch.com/nih/Differential-ion-accumulation-fluxes-in/10712547.html
Disrupted Homer scaffolds mediate abnormal mGluR5 function in a mouse model of fragile X syndrome.
Patch-Clamp Techniques. Peptides / pharmacology. Physics. Protein Synthesis Inhibitors / pharmacology. Rats. Rats, Long-Evans. ...http://www.biomedsearch.com/nih/Disrupted-Homer-scaffolds-mediate-abnormal/22267161.html
Nicardipine-sensitive Na+-mediated single channel currents in guinea-pig sinoatrial node pacemaker cells.
Patch-Clamp Techniques. Sinoatrial Node / cytology, drug effects*, metabolism*. Sodium / metabolism*. Chemical. ... The current density of ist was 0.33 pA pF-1 at -60 mV, as estimated from the number of channels per membrane patch, the open ...http://www.biomedsearch.com/nih/Nicardipine-sensitive-Na-mediated-single/10562335.html
Translocation of glutamate transporter subtype excitatory amino acid carrier 1 protein in kainic acid-induced rat epilepsy.
Patch-Clamp Techniques. Rats. Rats, Wistar. Receptors, AMPA / metabolism. Receptors, N-Methyl-D-Aspartate / metabolism. ...http://www.biomedsearch.com/nih/Translocation-glutamate-transporter-subtype-excitatory/12875997.html
"Optical Patch-clamping" |...
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. ... We now describe advances that greatly enhance the utility and resolution of this "optical patch-clamp" technique. Using an ... The membrane potential was clamped using a two-electrode voltage clamp (Gene Clamp 500; Axon Instruments). The voltage was held ... Such approaches hold potential as a complement to the well-established patch-clamp technique for single-channel recording ( ...http://jgp.rupress.org/content/126/3/179
Distinct cellular properties of identified dopaminergic and GABAergic neurons in the mouse ventral tegmental area.
Patch-Clamp Techniques / methods. Potassium / metabolism. Tyrosine 3-Monooxygenase / metabolism. Ventral Tegmental Area / ... During cell-attached patch-clamp recordings, GAD-GFP neurons fired brief action potentials that could be completely ... Under voltage-clamp the cationic current, I(h), was found in both types of neurons with considerable overlap in both amplitude ... Gene Knock-In Techniques / methods. Glutamate Decarboxylase / metabolism. Green Fluorescent Proteins / metabolism. ...http://www.biomedsearch.com/nih/Distinct-cellular-properties-identified-dopaminergic/21646409.html
Signal Transduction > Yale Cancer Center | Yale School of...
Clinical Trial; Patch-Clamp Techniques; Urinary Incontinence; Urothelium *Appt203.785.2815. *Appt Fax203.737.8035 ...http://medicine.yale.edu/cancer/research/people/signal.aspx
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