Techniques used to define human MHC antigens: polymerase chain reaction and oligonucleotide probes. - Immunology
Sequence-specific oligonucleotides (SSO) can be used to detect individual alleles with a high degree of accuracy by probing dot ... In addition, fine resolution of specificities such as the subtypes of DR4 is possible using a combination of SSO probes and ... can be identified with certainty by sequential hybridization to a series of 14 SSO probes. ... Sequence-specific oligonucleotides (SSO) can be used to detect individual alleles with a high degree of accuracy by probing dot ...
Daniel Oerther - Wikipedia
"The oligonucleotide probe database". Applied and Environmental Microbiology. 62 (10): 3557-3559. Bibcode:1996ApEnM..62.3557A. ...
US Patent Application for CASCADE OLIGONUCLEOTIDE DISPLACEMENT PROBES Patent Application (Application #20230295706 issued...
show an exemplary diagram of OSD cascade probes (also referred to as cascade OSD probes or cOSD probes). FIG. 1A. shows ... There are some key advantages of the cOSD probes strategy relative to other LAMP probe techniques. First, cOSD probes are ... the displaced probe strand mediates a second oligonucleotide strand displacement reaction with another probe of the set, ... duplex oligonucleotide of a cOSD probe pair hybridized to a third strand of a second hemi-duplex polynucleotide of a cOSD probe ...
Panmicrobial Oligonucleotide Array for Diagnosis of Infectious Diseases - Volume 13, Number 1-January 2007 - Emerging...
... as well as 11,479 16S rRNA bacterial probes, 1,120 18S rRNA fungal probes, and 848 18S rRNA parasite probes. A total of 300 ... Fluorescently labeled synthetic oligonucleotides complementary to the control probes were included in all hybridizations. ... The 10 positive probes aligned with all 8 MARV gene motifs represented on the array (Figure 1B). Only 4 (17%) of 23 probes were ... GreeneChipPm version 1.0 contained 29,495 probes that included probes comprising GreeneChipVr version 1.0, ...
Use of the Affymetrix Human GeneChip array and genomic DNA hybridisation probe selection to study ovine transcriptomes | animal...
Use of the Affymetrix Human GeneChip array and genomic DNA hybridisation probe selection to study ovine transcriptomes - Volume ... Pigeons: A Novel GUI Software for Analysing and Parsing High Density Heterologous Oligonucleotide Microarray Probe Level Data. ... Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics 4, 249-264. ... Optimising the analysis of transcript data using high density oligonucleotide arrays and genomic DNA-based probe selection. BMC ...
Oligonucleotide probes containing pyrimidine analogs reveal diminished hydrogen bonding capacity of the DNA adduct O-6-methyl-G...
Oligonucleotide probes containing pyrimidine analogs reveal diminished hydrogen bonding capacity of the DNA adduct O-6-methyl-G ...
Differential detection of type II methanotrophic bacteria in acidic peatlands using newly developed 16S rRNA-targeted...
... type II methanotrophic bacteria in acidic peatlands using newly developed 16S rRNA-targeted fluorescent oligonucleotide probes ... FUNCTIONAL GENE PROBE, RIBOSOMAL-RNA, METHYLOCELLA-PALUSTRIS, ACIDOPHILIC BACTERIUM, SP NOV., PEAT, DATABASE, CELLS; Title: ... This set of oligonucleotides included a genus-specific probe for Methylocystis (Mcyst-1432) and three species-specific probes ... M-450), with a genus-specific probe for Methylocystis spp. (Mcyst-1432), and with an additional probe (Mcyst-1261) that had ...
Combined analysis of microbial metagenomic and metatranscriptomic sequencing data to assess in situ physiological conditions in...
Transcriptome analysis of Escherichia coli using high-density oligonucleotide probe arrays journal, September 2002 * Tjaden, B. ... We used this approach to probe the environmental conditions experienced by Escherichia spp. in the gut of 4 premature infants, ... We used this approach to probe the environmental conditions experienced by Escherichia spp. in the gut of 4 premature infants, ...
Specificity Re-evaluation of Oligonucleotide Probes for the Detection of Marine Picoplankton by Tyramide Signal Amplification...
We confirmed the eukaryotic probes original conditions, and propose the Euk1209+NChlo01+Chlo02 probe mix to target the largest ... The probes were confronted to recent databases and hybridization conditions were tested against target strains matching ... We increased the hybridization stringency from 55 to 65% formamide for the Eub338+EubII+EubIII probe mix to be specific to the ... Experiences acquired through these investigations leads us to propose the use of seven steps protocol for complete FISH probe ...
Identification of Vibrio anguillarum in fish by using partial 16S rRNA sequences and a specific 16S rRNA oligonucleotide probe
From this sequence information we could design a 25-base-long oligonucleotide and use it as a specific probe for identification ... 1. Use of 16S ribosomal RNA probes for the detection of marine bacteria. Open this publication in new window or tab ,,Use of ... Strong, specific hybridization to the probe was observed for all V. anguillarum strains tested. Furthermore, no cross- ... 16S ribosomal RNA probes for the detection of marine bacteria. Rehnstam-Holm, Ann-Sofi. Department of Microbiology, Umeå ...
Submitting data to GTR in bulk using an excel file with a subset of fields
Multiplex Ligation-dependent Probe Amplification (MLPA). Next-Generation sequencing. Oligonucleotide Ligation Assay (OLA). ... Oligonucleotide hybridization-based DNA sequencing. PCR-RFLP with Southern hybridization. Protein truncation test ...
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We deliver oligonucleotides in various grades for research, diagnostic or commercial applications. ... We produce custom qPCR probes and labeled oligonucleotides with the original BHQ® molecules. ... By purchasing your BHQ® labeled oligonucleotides and qPCR Probes from Eurogentec you can use them for diagnostic and commercial ... Diagnostic Grade Oligonucleotides Research Grade Oligonucleotides. Research grade Oligonucleotides can be easily ordered via ...
Highly multiplexed oligonucleotide probe-ligation testing enables efficient extraction-free SARS-CoV-2 detection and viral...
Highly multiplexed oligonucleotide probe-ligation testing enables efficient extraction-free SARS-CoV-2 detection and viral ... Here we present a generalized version of the RNA-mediated oligonucleotide Annealing Selection and Ligation with next generation ... Given its extremely low per-sample cost, simple and automatable protocol and analytics, probe panel modularity, and massive ...
Interplay between CYP3A-mediated metabolism and polarized efflux of terfenadine and its metabolites in intestinal epithelial...
Ultra-high throughput single-cell analysis of proteins and RNAs by split-pool synthesis | Communications Biology
Padlock probes: circularizing oligonucleotides for localized DNA detection. Science https://doi.org/10.1126/science.7522346 ( ... SNAIL probe design. Between 2 and 5 SNAIL probe pairs were designed for each RNA target (Supplementary Data 11). The probe ... SNAIL probes were resuspended in DEPC-treated water at a concentration of 100 μM. SNAIL-padlock and SNAIL-splint probes for all ... This common oligonucleotide anneals to a second oligonucleotide with a 9 bp unique barcode identifier, termed Assayable ...
Viruses | Free Full-Text | Lyophilized Matrix Containing Ready-to-Use Primers and Probe Solution for Standardization of Real...
We describe here a process of preparation of oligonucleotide primers and hydrolysis probe in a single tube at predefined ... Table 1. Oligonucleotide primers and probes used in this study.. Primer/Probe. Sequence (5′-3′) a. Target. Position. Amplicon ( ... We describe here a process of preparation of oligonucleotide primers and hydrolysis probe in a single tube at predefined ... We describe here a process of preparation of oligonucleotide primers and hydrolysis probe in a single tube at predefined ...
Developmental suppression of schizophrenia-associated miR-137 alters sensorimotor function in zebrafish | Translational...
In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes. Nat Methods 2006; 3: 27-29. ... miRCURY miR-137 LNA probe for miRNA detection was obtained from Exiqon and prepared following the manufacturers instruction ( ... To transiently manipulate miR-137 activity in zebrafish, we utilized direct delivery of synthetic miR-137 oligonucleotide ... To further support these observations without the associated off-target effects of oligonucleotide-based manipulation, we ...
What are the Differences Between DNA and RNA Probes? - Enzo Life Sciences
What are the Differences Between DNA and RNA Probes? ... and synthetic oligonucleotide probes. Nucleic acid probes are ... Molecular probes can be broadly categorized into DNA probes and RNA probes, cDNA probes, ... BIO-PROBE® 3-Oligonucleotide labeling system reagent pack Dot Blot, ISH (in situ hybridization), Northern Blot, Southern Blot ... The probes consist of oligonucleotides designed for specific targets and offer unbiased signal amplification from the loop in ...
Analytical Chemistry | Vol 84, No 20
After the MB probe subsequently hybridizes with the target and functional probe, the oligonucleotide encapsulated Ag-NCs are ... as effective electrochemical probes. The functional oligonucleotide probe integrates both recognition sequence for ... Trace and Label-Free MicroRNA Detection Using Oligonucleotide Encapsulated Silver Nanoclusters as Probes. *. Haifeng. Dong. ... Demethylation of DNA probes initiates a degradation reaction of the probes by methylation-sensitive endonuclease Bsh 1236I and ...
A Complex Program of Striatal Gene Expression Induced by Dopaminergic Stimulation | Journal of Neuroscience
In all cases oligonucleotides were from Life Technologies. GenBank accession numbers used to design probes were as follows: arc ... Probe labeling and in situ hybridization.Procedures were as described in Gerfen et al. (1995). Briefly, RNA probes were ... Oligonucleotide probes were end-labeled using terminal deoxynucleotidyl transferase (Boehringer Mannheim) and 35S-dATP (New ... Generation of probe templates for known genes. For most of the known genes tested, 450-1000 bp templates were obtained by PCR ...
antisense oligonucleotide-mediated genetic therapy News Research Articles
JCI - Pathogenic human variant that dislocates GATA2 zinc fingers disrupts hematopoietic gene expression and signaling networks
WT GATA2 fingers did not stably bind the AGATAA probe; although the oligonucleotide was bound, stable complexes were unresolved ... Use of altered specificity mutants to probe a specific protein-protein interaction in differentiation: the GATA-1:FOG complex. ... EMSA with increasing protein concentrations and probes harboring single WGATAR sequences (AGATAA or TGATAA) of the GATA2- ... revealed that 9aa-Ins zinc fingers had reduced DNA binding capacity with TGATAA and double GATA probes. ...
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HPVC I R
This qualitative in-vitro diagnostics test uses oligonucleotide probes labeled with four different fluorescent dyes. The ...
Frontiers | DNA Methylation Cancer Biomarkers: Translation to the Clinic
Molecular Inversion Probes (MIP) are derivatives of padlock probes, although they contain a gap in the target sequence, which ... circularizing oligonucleotides for localized DNA detection. Science 265 (5181), 2085-2088. doi: 10.1126/science.7522346 ... Bisulfite padlock probes (BSPP) are an adaptation for the analysis of DNA methylation (Ball et al., 2009; Deng et al., 2009; ... Padlock probes are single stranded DNA molecules with two segments complementary to the target DNA connected by a linker ...
Microarray Technologies in the Diagnosis and Treatment of Head and Neck Cancer: Overview, Methods, Microarray Technologies
Each probe set consists of 16-20 probe pairs that represent specific coding regions for a given gene. The oligonucleotide ... These DNA probes are transferred as intact DNA strands, compared with individual bases of oligonucleotide microarrays. ... This process is repeated to build specific oligonucleotide sequences. Each chip contains thousands of probe sets, each ... Oligonucleotide microarrays. Oligonucleotide microarrays are manufactured by Affymetrix (Santa Clara, Calif) using ...
Oligonucleotide Synthesis Market Size, Share & Trends Analysis Report By Product (Column-based, Array-based, Services), By...
Oligonucleotide Synthesis Market Size, Share & Trends Analysis Report By Product (Column-based, Array-based, Services), By ... Global Oligonucleotide Synthesis Market by Product (Equipment, Linkers & Adaptor, Probes), Type (Custom, Pre-designed), ... Oligonucleotides. 4.2.1. Oligonucleotides Market, 2018 - 2030 (USD Million). 4.2.2. Oligonucleotides, By Product Type. 4.2.2.1 ... Table 19 Europe oligonucleotide synthesis market, by end user, 2018 - 2030 (USD Million). Table 20 UK oligonucleotide synthesis ...
Minor Groove Binder (MGB) Probes | LGC Biosearch Technologies
... hydrolysis probes consisting of a 5 fluorescent reporter dye and a 3 Eclipse Dark Quencher (EDQ) conjugated to a MGB moiety. ... Oligonucleotide resuspension. Re-suspend the dried probes to make stock solutions. We recommend creating stocks of 100 µM. TE ... probe or. MGB probe dual-labeled BHQ probe or BHQplus® probe or. MGB probe instead. ... MGB probe technology overview. MGB Probes have several advantages over standard DNA probes, making this probe type a powerful, ...
Cancers | Free Full-Text | Transcription Factor RBPJL Is Able to Repress Notch Target Gene Expression but Is Non-Responsive to...
The 32P-labeled oligonucleotide (s) FO233F/R was used as probe. (B) Quality of RBPJL proteins after in vitro translation was ... The 32P-labeled oligonucleotide (s) FO233F/R was used as probe. (B) Quality of RBPJL proteins after in vitro translation was ... For the binding reaction, 10 ng (0.02 U) poly(dI-dC) (GE Healthcare) and approximately 0.5 ng 32P-labeled oligonucleotides were ... The sequence of the double-stranded oligonucleotide FO233 (see Table S1) corresponded to the two RBPJ-binding sites within the ...