*  Team:Freiburg Bioware/Project/Methods - 2010.igem.org
Renaturation and hybridization reactions lead to the pairing of complementary single-stranded nucleic acids. The main technique ... GelredTM is a fluorescent nucleic acid gel stain which intercalates with nucleic acid and replaces the usage of the highly ... SYBR Green is a nucleic acid stain which preferentially binds within the minor groove of double-stranded DNA. By excitation at ... quantify and purify nucleic acids. The usage of the two types of gels depends on the size of the fragments. Whereas an agarose ...
  http://2010.igem.org/Team:Freiburg_Bioware/Project/Methods
*  Patent US5667972 - Method of sequencing of genoms by hybridization of oligonucleotide probes - Google Patents
Wetmur, Hybridization and Renaturation Kinetics of Nucleic Acids, Annual Review of Biophysics and Bioengineering, pp. 337 361 ( ... Nucleic acid arrays. US6291181. Oct 5, 1999. Sep 18, 2001. Affymetrix, Inc.. Nucleic acid adapters containing a type IIs ... Methods of identifying nucleic acid probes to quantify the expression of a target nucleic acid. ... Gingeras and Davis, "Hybridization properties of immobilized nucleic acids," Nucleic Acids Research, 15:5373-5390 (1987).. ...
  http://www.google.com/patents/US5667972?dq=patent:6144888
*  Plus it
Jean D, Gendron D, Delbecchi L, Bourgaux P (1997) p53-mediated DNA renaturation can mimic strand exchange. Nucleic Acids Res 25 ... Oberosler P, Hloch P, Ramsperger U, Stahl H (1993) p53-catalyzed annealing of complementary single-stranded nucleic acids. EMBO ... p53 binds single-stranded DNA ends and catalyzes DNA renaturation and strand transfer. Proc Natl Acad Sci USA 91:413-417. ... but the flanking amino acid sequences are identical in both species (Pecker et al., 1996); hence, the site is conserved. To ...
  http://www.jneurosci.org/content/26/29/7767
*  EP0167366B1 - Accelerated nucleic acid reassociation method - Google Patents
Renaturation generally refers to the formation of a double stranded nucleic acid molecule from two single stranded molecules ... of the single stranded nucleic acid will associate to form double stranded nucleic acid molecules. At higher nucleic acid ... nucleic acid molecule and the second single-stranded nucleic acid molecule is mixed with a solution containing the nucleic acid ... their nucleic acid with a marked probe comprising nucleic acid molecules complementary to the RNA or other nucleic acid ...
  https://patents.google.com/patent/EP0167366B1/en
*  Dimethyl sulfoxide | 67-68-5
Dimethyl sulfoxide (1-10%) has been shown to accelerate strand renaturation and is believed to give the nucleic acid thermal ... Incompatible with a very wide range of materials, including acid chlorides, strong acids, strong oxidizing agents, strong ... Identification] Take 1.5 mL of this product and add to it slowly of 2.5 mL cooled hydroiodic acid drop wise; rapidly filter it ... It can lead to explosion when mixing the dimethyl sulfoxide with certain substance such as sodium hydride, periodic acid or ...
  http://www.chemicalbook.com/ChemicalProductProperty_EN_CB7854105.htm
*  Molecular Modeling of Nucleic Acid Structure: Electrostatics and Solvation - Current Protocols
This unit presents an overview of computer simulation techniques as applied to nucleic acid systems, ranging from simple in ... A mesoscale model of DNA and its renaturation. Biophys. J. 96:1675‐1690. ... Keywords: nucleic acid chemistry; nucleic acid structure and folding; structural analysis of biomolecules; experimental ... Nucleic Acids Res. 38:299‐313.. Lawrence, C.P. and Skinner, J.L. 2003. Flexible TIP4P model for molecular dynamics simulation ...
  http://www.currentprotocols.com/WileyCDA/CPUnit/refId-nc0709.html?quicktabs_cp=toc
*  DIMETHYL SULFOXIDE - CAS - 67-68-5 (02190186) - MP Biomedicals
Has been shown to accelerate strand renaturation (1-10% concentration) and is believed to give the nucleic acid thermal ... MPure-12 Nucleic Acid. Purification System. Lysing. Beads and Tubes. DNA, RNA & Protein. Isolation Kits. FastPrep Homogenizer. ... Amino Acids and Enzymes Buffers & Salts Nucleosides and Nucleotides Specialty Chemicals >> View All Empowered Products. New ... Amino Acids and Enzymes Buffers and Salts Nucleosides and Nucleotides Specialty Chemicals >> View All Empowered Products ...
  https://www.mpbio.com/product.php?pid=02190186&country=223
*  Patent US6440677 - Nucleic acid affinity columns - Google Patents
... nucleic acid sequence. b) amplifying the multiplicity of oligonucleotides to provide a pool of amplified nucleic acids; and c) ... nucleic acid affinity ligands allowing the simultaneous selection and removal of a large number of preselected nucleic acids ... In general the methods involve the steps of a) providing a nucleic acid amplification template array comprising a surface to ... attaching the pool of nucleic acids to a solid support. ... Nucleic acid affinity matrices that bear a large number of ...
  http://www.google.com.au/patents/US6440677
*  A Functional Approach to Transcriptome Profiling: Linking Gene Expression Patterns to Metabolites that Matter | SpringerLink
Studies on nucleic acid reassociation kinetics. 5. Effects of disparity in tracer and driver fragment lengths. Nucl Acids Rese ... compared to rate of DNA renaturation. 3. Proc Natl Acad Sci USA 74, 1020-1023CrossRefGoogle Scholar ... Hong YK, Sohn CH, Lee KW, Kim HG (1997) Nucleic acid extraction from seaweed tissues for polymerase chain reaction. J Mar ... Galau GA, Britten RJ, Davidson EH (1977) Studies on nucleic acid reassociation kinetics-rate of hybridization excess of RNA ...
  https://link.springer.com/article/10.1007%2Fs10126-007-9008-2
*  Eukaryotic Gene Expression Problem Set
Problem 5: DNA-DNA renaturation and DNA-RNA hybridization Which statement is NOT true about nucleic acid hybridization? A. it ...
  http://biology.arizona.edu/molecular_bio/problem_sets/mol_genetics_of_eukaryotes/05t.html
*  Nucleic acid quantitation - Wikipedia
"Studies on the spectrophotometric determination of DNA hybridization from renaturation rates". Systematic and Applied ... The "A260 unit" is used as a quantity measure for nucleic acids. One A260 unit is the amount of nucleic acid contained in 1 mL ... 280 ratio in a nucleic acid solution. 260:280 ratio has high sensitivity for nucleic acid contamination in protein: 260:230 ... units to nucleic acid concentration conversion factors are used to convert OD to concentration of unknown nucleic acid samples ...
  https://en.wikipedia.org/wiki/Nucleic_acid_quantitation
*  Muteins of the c5a anaphylatoxin, nucleic acid molecules encoding such muteins, and pharmaceutical uses of muteins of the c5a...
... to nucleic acid molecules comprising a nucleotide sequence encoding such muteins of C5a anaph ... In this case the polypeptide can, for instance, be produced in form of inclusion bodies, followed by renaturation in vitro (cf ... The nucleic acid molecule of any of claims 20 to 23 comprised in a vector. 25. A host cell containing a nucleic acid molecule ... A nucleic acid molecule such as DNA is regarded to be "capable of expressing a nucleic acid molecule or a coding nucleotide ...
  http://www.freepatentsonline.com/y2006/0052294.html
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Gambi t in situ hybridization Renaturation of a radioactive probe nucleic acid to the DNA or RNA forex gambit mkv a cell; used ... How is the sequence of amino acids in forex gambit mkv protein encoded in the sequence of nucleotides in a messenger RNA. DnaK ...
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*  Pure Biologics, Ltd. - Science Exchange
Proprietary refolding screens for IB renaturation;. • Nucleic acid and pyrogenremoval;. • Constant activity and purity... Show ... Proprietary refolding screens for IB renaturation;. • Nucleic acid and pyrogenremoval;. • Constant activity and purity ...
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*  Interaction of Metronidazole with Nucleic Acids in Vitro | Molecular Pharmacology
... and did not affect the melting temperature or renaturation profile of calf thymus DNA. While 15-30% of bound 14C was released ... 14C]Metronidazole was incubated with nucleic acid under various conditions, the nucleic acid and associated 14C were separated ... Interaction of Metronidazole with Nucleic Acids in Vitro. NICHOLAS F. LARUSSO, MARIA TOMASZ, MIKLÓS MÜLLER and ROSELYN LIPMAN ... Interaction of Metronidazole with Nucleic Acids in Vitro. NICHOLAS F. LARUSSO, MARIA TOMASZ, MIKLÓS MÜLLER and ROSELYN LIPMAN ...
  http://molpharm.aspetjournals.org/content/13/5/872
*  Nucleic acid thermodynamics - Wikipedia
The term is also often used to describe the reformation (renaturation) of reverse-complementary strands that were separated by ... Nucleic acid thermodynamics is the study of how temperature affects the nucleic acid structure of double-stranded DNA (dsDNA). ... occurs when half of the double-stranded nucleic acid has dissociated. If no additional nucleic acids are present, then [A], [B ... elegantly describe the thermodynamic parameters for forming double-stranded nucleic acid AB from single-stranded nucleic acids ...
  https://en.wikipedia.org/wiki/Nucleic_acid_thermodynamics
*  Sandwalk: Paul Doty (1920 - 2011) and DNA Renaturation
According to my old copy of Principles of Nucleic Acid Structure (Saenger, 1984):. 'Stacked arrangements are also dominant in ... "Thermal Renaturation of Deoxyribonucleic Acids." This was the first time that the renaturation of complex DNA had been studied ... Marmur, J. and Doty, P. (1962) Thermal Renaturation of Deoxyribonucleic Acids. J. Mol. Biol. 3:585-594. [PubMed] [doi: 10.1016/ ... This is called renaturation.. The key parameters for renaturation are time, temperature, and ionic strength and one of the key ...
  http://sandwalk.blogspot.ca/2012/01/paul-doty-1920-2011-and-dna.html
*  Patent US6960436 - Quantitative methylation detection in DNA samples - Google Patents
Assay for detecting methylation changes in nucleic acids using an intercalating nucleic acid. ... thus preventing the diffusion and renaturation of the DNA (bisulfite only reacts with single-stranded DNA), and which replaces ... of a nucleic acid sequence by repetitive cycles of annealing and extending primer to single stranded nucleic acids followed by ... Treatment of nucleic acid. US20070020667 *. Jun 29, 2006. Jan 25, 2007. Ruff David W. Methods and compositions for amplifying ...
  http://www.google.com/patents/US6960436?dq=7,194,691
*  Patent WO2001081622A2 - Diagnosis of diseases associated with dna repair - Google Patents
2. A nucleic acid comprising a sequence at least 18 base pairs in length of a segment of the chemically pretreated DNA of genes ... thus preventing the diffusion and renaturation of the DNA (bisulfite only reacts with single-stranded DNA), and which replaces ... 1. A nucleic acid comprising a sequence at least 18 bases in length of a segment of the chemically pretreated DNA of genes ... Nucleic Acids Res. 1995 Apr 25;23(8): 1367-73). The coupling of a charge tag to this modified DNA results in an increase in ...
  http://www.google.com.au/patents/WO2001081622A2
*  Masters M.Sc in Bioinformatics Coimbatore Bharathiar University | Emagister
NUCLEIC ACID, IMMUNOLOGY AND PHARMACOLOGY Subject description : This paper includes the isolation of protein, nucleic acid and ... form of DNA, denaturation and renaturation of DNA Lipids-Structure and function of Fatty acids, Triacylglycerols, sphingolipids ... Nucleic Acid Lab 7. Isolation of DNA from microbial and animal cells 8. Isolation of RNA from bakers yeast 9. Isolation of ... Stereochemistry of proteins and nucleic acids.Subject title : BIOPHYSICS AND CRYSTALLOGRAPHY Course number : 07BIC06 Number of ...
  https://www.emagister.in/m_sc_bioinformatics_courses-ec2631497.htm
*  NAVER Academic | Monocistronic transcription is the physiological mechanism of sea urchin embryonic histone gene expression.
We have subcloned into pBR322 deoxyribonucleic acid (DNA) sequences mapping either in the coding region, the 5' spacer, or the ... coding regions and their intervening spacer sequences are transcribed into large polycistronic messenger ribonucleic acid (RNA ... Studies on nucleic acid reassociation kinetics: rate of hybridization of excess RNA with DNA, compared to the rate of DNA ... renaturation. (1977) G A Galau et al. Proceedings of the National Academy of Sciences of the United States of America ...
  https://academic.naver.com/article.naver?doc_id=249862615
*  Nano-Scale Biosensors - Patent application - Devices systems and methods for detecting nucleic acid hybridization
... forming a double-stranded nucleic acid sequence, including the reformation (renaturation) of complementary strands that were ... gradient focusing of the target nucleic acids; [0012]use of surface bound hairpin loop nucleic acid probes that are capable of ... 0170]The invention provides devices, systems, and methods for detecting nucleic acid hybridization, including single nucleic ... Devices, systems, and methods for detecting nucleic acid hybridization, including single nucleic base mutations at low ...
  http://www.patentsencyclopedia.com/app/20100201381
*  Handling Metalloproteinases - Current Protocols
... usually the carboxyl group of a glutamic acid side chain ... Nucleic Acids Res. 42:503‐509. doi: 10.1093/nar/gkt953.. Reyda ... cDNA cloning, bacterial expression, in vitro renaturation and affinity purification of the zinc endopeptidase astacin. Biochem ... 10% glacial acetic acid: 50 g of glacial acetic acid in 227 ml of H 2O ... acid); Dabcyl = 4‐((4‐(dimethylamino)phenyl)azo)benzoic acid)]; store protected from light at up to several months at -20°C ...
  http://www.currentprotocols.com/WileyCDA/CPUnit/refId-ps2116.html
*  Book Review The Chemistry of Nucleosides and Nucleotides. By A. M. Michelson
Chapters 6-9 are devoted to high molecular-weight nucleic acids. The organic chemistry of nucleic acids is dealt with first, ... and the processes occurring during their denaturation and renaturation. I n the concluding chapter a short survey is given of ... and mixed anhydrides with sulfuric acid, carbonic acid, and organic acids. After the section o n the chemical syntheses of ... As already indicated by the title of the book, it is the chemistry of the nucleic acids that is emphasised, in accordance with ...
  https://www.docme.ru/doc/1910851/book-review--the-chemistry-of-nucleosides-and-nucleotides..
*  Binary options demo without deposit
... preventing renaturation of DNA black scholes model binary option partial denaturation of the nucleic acid strands (10,18). ... Nucleic Acids Res. American Society for Microbiology, Washington, D. 5minat30°C. Neurosci.A. Kleckner. Since the vectors ...
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