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*  GENETIC TEST SYSTEM - Patent application
16, two nucleic acid extraction devices 32a and 32b are added; however, the number of the nucleic acid extraction devices to be ... However, the PCR involves a temperature cycle consisting of denaturation reaction, annealing reaction, and extension reaction, ... 0084] 32a (first) additional device for nucleic acid extraction. [0085] 32b (second) additional device for nucleic acid ... Nucleic acid amplification/detection device and nucleic acid inspection device using same. ...
  http://www.patentsencyclopedia.com/app/20140193893
*  Patent US5200313 - Nucleic acid hybridization assay employing detectable anti-hybrid antibodies - Google Patents
No immobilization or labeling of sample nucleic acids is necessary and hybridization can be performed entirely in solution. ... selective for binding the hybrids in the presence of the single stranded sample and probe nucleic acids. ... A nucleic acid hybridization assay employing an immobilized or immobilizable polynucleotide probe selected to form DNA.RNA or ... and if necessary first to release nucleic acids from cells. Denaturation of nucleic acids is preferably accomplished by heating ...
  http://www.google.ca/patents/US5200313
*  Human viruses in water - Wikipedia
The rate of protein, nucleic acid denaturation and chemical reactions that destroy the viral capsid are increased at higher ...
  https://en.wikipedia.org/wiki/Human_viruses_in_water
*  Denaturation (biochemistry) - Wikipedia
Acidic nucleic acid denaturants include: Acetic acid HCl Nitric Acid Basic nucleic acid denaturants include: NaOH Other nucleic ... which promotes denaturation of nucleic acids in order to eliminate the influence of nucleic acid shape on their electrophoretic ... Acetic acid Trichloroacetic acid 12% in water Sulfosalicylic acid Bases work similarly to acids in denaturation. They include: ... Other than denaturation by heat, nucleic acids can undergo the denaturation process through various chemical agents such as ...
  https://en.wikipedia.org/wiki/Denaturation_(biochemistry)
*  Nucleic acid thermodynamics - Wikipedia
Most commonly, the pairs of nucleic bases A=T and G≡C are formed, of which the latter is more stable. DNA denaturation, also ... Nucleic acid thermodynamics is the study of how temperature affects the nucleic acid structure of double-stranded DNA (dsDNA). ... occurs when half of the double-stranded nucleic acid has dissociated. If no additional nucleic acids are present, then [A], [B ... elegantly describe the thermodynamic parameters for forming double-stranded nucleic acid AB from single-stranded nucleic acids ...
  https://en.wikipedia.org/wiki/Nucleic_acid_thermodynamics
*  Slow virus - Wikipedia
No evidence of nucleic acid has been found in any prion particle studied. Treatments that destroy protein, like denaturation, ... Once it was discovered that prions were the real cause of TSEs and that prions contained no nucleic acid, the term virions was ... but treatments that destroy nucleic acids, like UV radiation, do not destroy prion infectivity. The prion protein is known as ... The infectious form of PrP has the same amino acid sequence and the same post-translational modifications as the normal form, ...
  https://en.wikipedia.org/wiki/Slow_virus
*  Glossary of biology - Wikipedia
dehydration reaction . denaturation A process in which proteins or nucleic acids lose the quaternary, tertiary and secondary ... nucleic acid . nucleic acid sequence . nucleobase . nucleoid . nucleolus . nucleotide . Contents: 0-9 A B C D E F G H I J K L M ... DNA An abbreviation for deoxyribonucleic acid; a type of nucleic acid that serves as the fundamental hereditary material in ... Both RNA and DNA are nucleic acids, which use base pairs of nucleotides as a complementary language. transfer RNA . translation ...
  https://en.wikipedia.org/wiki/Glossary_of_biology
*  Polyacrylamide gel electrophoresis - Wikipedia
For nucleic acids, urea is the most commonly used denaturant. For proteins, sodium dodecyl sulfate (SDS) is an anionic ... This denaturation, which is referred to as reconstructive denaturation, is not accomplished by the total linearization of the ... Ethidium bromide binds nucleic acid chains through the process of Intercalation. While Ethidium bromide is a popular stain it ... Ethidium bromide (EtBr) is a popular nucleic acid stain. EtBr allows one to easily visualize DNA or RNA on a gel as EtBr ...
  https://en.wikipedia.org/wiki/Polyacrylamide_gel_electrophoresis
*  Deactivation - Wikipedia
Deactivation may refer to; Protein denaturation, the process of disrupting the structure of proteins or nucleic acids Drug ...
  https://en.wikipedia.org/wiki/Deactivation
*  Christian B. Anfinsen - Wikipedia
He was also a pioneer of ideas in the area of nucleic acid compaction. In 1961, he showed that ribonuclease could be refolded ... after denaturation while preserving enzyme activity, thereby suggesting that all the information required by protein to adopt ... especially concerning the connection between the amino acid sequence and the biologically active conformation (see Anfinsen's ... its final conformation is encoded in its amino-acid sequence. He belonged to the National Academy of Sciences (USA), the Royal ...
  https://en.wikipedia.org/wiki/Christian_B._Anfinsen
*  Histology - Wikipedia
... and DNA as well as denaturation and modification of proteins in tissues. However, extraction and analysis of nucleic acids and ... Hematoxylin, a basic dye, stains nuclei blue due to an affinity to nucleic acids in the cell nucleus; eosin, an acidic dye, ... Because Formalin-fixed, paraffin-embedded (FFPE) tissues may be stored indefinitely at room temperature, and nucleic acids ( ... or sites to which radiolabeled nucleic acid probes bind in in situ hybridization. For autoradiography on a microscopic level, ...
  https://en.wikipedia.org/wiki/Histology
*  William Dale Phillips - Wikipedia
He also had a strong interest in the NMR and ESR of nucleic acids and other biological macromlecules. To further his ... Origins of contact-shifted resonances and denaturation by dimethyl sulfoxide. Annals of the New York Academy of Sciences (1973 ... Nucleic acids; a nuclear magnetic resonance (NMR) study. Science (Washington, DC, United States) (1964), 144(3623), 1234-7. ... Nuclear magnetic resonance determination of thymine nearest neighbor base requency ratios in deoxyribonucleic acid. Journal of ...
  https://en.wikipedia.org/wiki/William_Dale_Phillips
*  René Thomas (biologist) - Wikipedia
At ULB, René Thomas attended lectures by Jean Brachet, who pioneered the field of nucleic acids (DNA and RNA) and their role in ... Under Brachet's supervision, René Thomas prepared and defended a PhD thesis on the denaturation of DNA in 1952. After two years ... Melting this secondary structure was coined DNA denaturation, by analogy with the similar process long known for proteins. Once ... DNA denaturation could be understood as the unwinding of the double helix. It became fundamental in all processes that use DNA ...
  https://en.wikipedia.org/wiki/Ren%C3%A9_Thomas_(biologist)
*  Roger Wartell - Wikipedia
Frontiers in Nucleic Acids. ACS Symposium Series. 1082. p. 111. doi:10.1021/bk-2011-1082.ch007. ISBN 0-8412-2623-7. Wartell, R ... M.; Benight, A. S. (1985). "Thermal denaturation of DNA molecules: A comparison of theory with experiment". Physics Reports. ... Nucleic Acids Research. 30 (17): 3662-3671. doi:10.1093/nar/gkf508. PMC 137430 . PMID 12202750. "Roger Wartell". Retrieved 17 ...
  https://en.wikipedia.org/wiki/Roger_Wartell
*  Melting curve analysis - Wikipedia
Nucleic Acids Research, 39 (8): e52-e52, doi:10.1093/nar/gkr035, PMC 3082908 , PMID 21297115. Ririe, KM; Rasmussen, RP; Wittwer ... "High-resolution thermal denaturation of DNA. I. Theoretical and practical considerations for the resolution of thermal ... the conformation and the modifications of DNA and RNA Nucleic acid thermodynamics Ansevin, A.T.; Vizard, D.L.; Brown, B.W.; ...
  https://en.wikipedia.org/wiki/Melting_curve_analysis
*  Prion - Wikipedia
The 'virino hypothesis' postulates that the TSE agent is a foreign, self replicating nucleic acid or nucleic acid fragment ... Sterilizing prions, therefore, requires the denaturation of the protein to a state in which the molecule is no longer able to ... Infectious particles possessing nucleic acid are dependent upon it to direct their continued replication. Prions, however, are ... Prior to the discovery of prions, it was thought that all pathogens used nucleic acids to direct their replication. The " ...
  https://en.wikipedia.org/wiki/Prion
*  Gel electrophoresis - Wikipedia
Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of ... For full denaturation of proteins, it is also necessary to reduce the covalent disulfide bonds that stabilize their tertiary ... When separating larger nucleic acids (greater than a few hundred bases), the preferred matrix is purified agarose. In both ... Nucleic acids are often denatured by including urea in the buffer, while proteins are denatured using sodium dodecyl sulfate, ...
  https://en.wikipedia.org/wiki/Gel_electrophoresis
*  Overlap extension polymerase chain reaction - Wikipedia
Nucleic Acids Res. 16 (15): 7351-67. doi:10.1093/nar/16.15.7351. PMC 338413 . PMID 3045756. Molecular and cellular biology ... After mixing and denaturation, the strands are allowed to anneal to produce three different combinations as detailed in the ...
  https://en.wikipedia.org/wiki/Overlap_extension_polymerase_chain_reaction
*  Transfer RNA - Wikipedia
Nucleic Acids Res. Mol. Biol. 22, 1-16 Green, R., and Noller, H. F. (1997) Annu. Rev. Biochem. 66, 679-716 Aebi M, Kirchner G, ... Felsenfeld G, Cantoni G; Cantoni (1964). "Use of thermal denaturation studies to investigate the base sequence of yeast serine ... 2014). "tRFdb: a database for transfer RNA fragments". Nucleic Acids Res. 43: D141-D145. doi:10.1093/nar/gku1138. PMC 4383946 ... Goodenbour, J. M.; Pan, T. (29 October 2006). "Diversity of tRNA genes in eukaryotes". Nucleic Acids Research. 34 (21): 6137- ...
  https://en.wikipedia.org/wiki/Transfer_RNA
*  Chaotropic agent - Wikipedia
... nucleic acids) by weakening the hydrophobic effect. For example, a chaotropic agent reduces the amount of order in the ... and may cause its denaturation. Conversely, an antichaotropic agent is a molecule in an aqueous solution that will increase the ... macromolecules such as proteins and nucleic acids (e.g. DNA and RNA). Chaotropic solutes increase the entropy of the system by ... Tertiary protein folding is dependent on hydrophobic forces from amino acids throughout the sequence of the protein. Chaotropic ...
  https://en.wikipedia.org/wiki/Chaotropic_agent
*  Alpha-lactalbumin - Wikipedia
Nucleic Acids Res. 9 (1): 65-84. doi:10.1093/nar/9.1.65. PMC 326669 . PMID 6163135. Hall L, Emery DC, Davies MS, Parker D, ... As a result, pure α-lactalbumin will not form gels upon denaturation and acidification. The sequence comparison of α- ... Nucleic Acids Res. 10 (11): 3503-3515. doi:10.1093/nar/10.11.3503. PMC 320727 . PMID 6285305. Håkansson A, Zhivotovsky B, ... The structure of alpha-lactalbumin is well known and is composed of 123 amino acids and 4 disulfide bridges. The molecular ...
  https://en.wikipedia.org/wiki/Alpha-lactalbumin
*  Nano differential scanning fluorimetry - Wikipedia
Nucleic Acids Research. 43: gkv1053. doi:10.1093/nar/gkv1053. ISSN 0305-1048. PMC 4787788 . PMID 26481363. Linke, P.; Amaning, ... "Thermal denaturation assays in chemical biology". Assay Drug Dev Technol. 10: 128-36. doi:10.1089/adt.2011.0390. PMID 22066913 ...
  https://en.wikipedia.org/wiki/Nano_differential_scanning_fluorimetry
*  Guanidinium thiocyanate - Wikipedia
... although it is most commonly used as a Nucleic Acid protector in the extraction of DNA and RNA. Note: this compound may also be ... This application is presumably based on its denaturation activity. Guanidinium thiocyanate is also used to lyse cells and virus ... This is because guanidinium is the conjugate acid of guanidine and is called the guanidinium cation, [CH6N3]+. Guanidinium ...
  https://en.wikipedia.org/wiki/Guanidinium_thiocyanate
*  Optical mapping - Wikipedia
Nucleic Acids Research. 42 (7): e50-e50. doi:10.1093/nar/gkt1406. ISSN 0305-1048. PMC 3985630 . PMID 24452797. Dimalanta, E.T. ... "Single-molecule denaturation mapping of DNA in nanofluidic channels". Proceedings of the National Academy of Sciences. 107 (30 ... Nucleic Acids Research. 42 (15): e118-e118. doi:10.1093/nar/gku556. ISSN 0305-1048. PMC 4150756 . PMID 25013180. Grunwald, ... Nucleic Acids Research. 43 (18): e117-e117. doi:10.1093/nar/gkv563. ISSN 0305-1048. PMC 4605287 . PMID 26019180. Vranken, ...
  https://en.wikipedia.org/wiki/Optical_mapping
*  Protein engineering - Wikipedia
Gene synthesis Nucleic acid analogues Protein structure prediction software Proteomics Proteome SCOPE (protein engineering) ... This PCR based method begins with an initial denaturation of the template, followed by annealing of primers and a short ... that converts amino acid sequences into a sequence composed of volume and polarity values for each amino acid residue. This new ... These analyses can help to identify hot spot amino acids that can serve as the target sites for mutations. Multiple sequence ...
  https://en.wikipedia.org/wiki/Protein_engineering