Ca2+-regulatory proteins in cardiomyocytes from the right ventricle in children with congenital heart disease | Journal of...
Ronkainen VP, Skoumal R, Tavi P: Hypoxia and HIF-1 suppress SERCA2a expression in embryonic cardiac myocytes through two ... Bers DM: Cardiac excitation-contraction coupling. Nature. 2002, 415: 198-205. 10.1038/415198a.View ArticlePubMedGoogle Scholar ... SERCA2a (the cardiac isoform of SERCA) is a Ca2+-ATPase pump in SR that pumps Ca2+ back to SR during myocardial relaxation [29 ... Subunit expression of the cardiac L-type calcium channel is differentially regulated in diastolic heart failure of the cardiac ...https://translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-10-67
Trophic Effect of Human Pericardial Fluid on Adult Cardiac Myocytes | Circulation Research
Cardiac Myocyte Preparation and Culture. Cardiac myocytes were obtained from 2-month-old male Wistar rats (weight, 200 g) as ... including cardiac myocytes11 and endothelial42 and smooth muscle cells.43 TGF-β and FGF2 have been found in cardiac myocytes ... 6-8 FGF2 and TGF-β are found in cardiac myocytes and in cardiac interstitium9,10 after cardiac overload.7,8,11 Although the ... The cardiac β-MyHC is predominantly expressed in cardiac ventricular myocytes throughout embryonic and fetal development in the ...http://circres.ahajournals.org/content/81/5/679
Discrete microdomains with high concentration of cAMP in stimulated rat neonatal cardiac myocytes. - Oxford Neuroscience
In cardiac myocytes and many other cell types, however, cAMP transduces the signal generated upon stimulation of various ... Here, we show that in neonatal rat cardiac myocytes, beta-adrenergic stimulation generates multiple microdomains with increased ... is the most important modulator of sympathetic control over cardiac contractility. ... In cardiac myocytes and many other cell types, however, cAMP transduces the signal generated upon stimulation of various ...https://www.neuroscience.ox.ac.uk/publications/237329
Angiotensin II Induces Cardiac Phenotypic Modulation and Remodeling In Vivo in Rats | Hypertension
17 Fibronectin is localized along the surface of cardiac myocytes, connects cardiac myocytes to perimyocytic collagen, and is ... Cardiac sarcomeric actin is also composed of two isoforms, cardiac and skeletal α-actins.18 Cardiac α-actin is predominantly ... mechanical stretch-induced hypertrophy of cardiac myocytes is mediated by autocrine release of Ang II from cardiac myocytes,10 ... increase in cardiac myocyte size) but also by qualitative changes, including a shift to the fetal phenotype of myocytes5 12 13 ...http://hyper.ahajournals.org/content/25/6/1252?ijkey=fc355b0b00cfa7b0f95802bc159564a09f7bfc6f&keytype2=tf_ipsecsha
Mitochondrial and sarcolemmal Ca2+ transport reduce [Ca2+]i during caffeine contractures in rabbit cardiac myocytes. J Physiol ... Cardiac myocyte calcium transport in phospholamban knockout mouse: relaxation and endogenous CaMKII effects. Am J Physiol Heart ... Embryonic lethality and abnormal cardiac myocytes in mice lacking ryanodine receptor type 2. EMBO J 17: 3309-3316, 1998. ... Ascorbic acid enhances differentiation of embryonic stem cells into cardiac myocytes. Circulation 107: 1912-1916, 2003. ...http://ajpcell.physiology.org/content/297/3/C732
The Molecular Basis for Distinct β-Adrenergic Receptor Subtype Actions in Cardiomyocytes | Circulation Research
Cardiac myocytes express mRNA for ten RGS proteins: changes in RGS mRNA expression in ventricular myocytes and cultured atria. ... Functional coupling of the β2-adrenoceptor to a pertussis toxin-sensitive G protein in cardiac myocytes. Mol Pharmacol. 1995;47 ... However, the mechanism(s) underlying β2-AR actions in cardiac myocytes is less straightforward. Whereas β2-ARs also can link to ... The lethal effects of cytokine-induced nitric oxide on cardiac myocytes are blocked by nitric oxide synthase antagonism or ...http://circres.ahajournals.org/content/85/11/1101.long
Downregulation of cardiac myocyte Na+-K+-ATPase by adenovirus-mediated expression of an α-subunit fragment | Heart and...
Neonatal rat cardiac myocytes were prepared and cultured as described earlier (15, 26). Myocytes were isolated from ventricles ... Downregulations of the α1- and α3-subunit mRNAs in neonatal cardiac myocytes transfected with the α2-adenovirus. Myocytes were ... and the α3-subunit proteins in neonatal cardiac myocytes transfected with the α2-adenovirus. Myocytes were transfected with the ... Downregulation of cardiac myocyte Na+-K+-ATPase by adenovirus-mediated expression of an α-subunit fragment. Peter Kometiani, ...http://ajpheart.physiology.org/content/280/3/H1415.long
Cell Logic for Dual Coupling of a Single Class of Receptors to Gs and Gi Proteins | Circulation Research
... effects on cardiac myocyte apoptosis. In cultured adult rat cardiac myocytes, stimulation of β1AR, but not β2AR, induces ... Coupling of β2-adrenoceptor to Gi proteins and its physiological relevance in murine cardiac myocytes. Circ Res. 1999;84:43-52. ... Although activation of β2AR-coupled Gi protects cardiac myocytes against apoptosis, an imbalance of β2AR-initiated Gs and Gi ... Functional coupling of the β2-adrenoceptor to a pertussis toxin-sensitive G protein in cardiac myocytes. Mol Pharmacol. 1995;47 ...http://circres.ahajournals.org/content/87/8/635
Spark-Induced Sparks As a Mechanism of Intracellular Calcium Alternans in Cardiac Myocytes | Circulation Research
Analysis of ryanodine receptor clusters in rat and human cardiac myocytes. Proc Natl Acad Sci U S A. 2007; 104: 14958-14963. ... Spark-Induced Sparks As a Mechanism of Intracellular Calcium Alternans in Cardiac Myocytes. Robert Rovetti, Xiaohua Cui, Alan ... Calcium sparks and [Ca2+]i waves in cardiac myocytes. Am J Physiol. 1996; 270: C148-C159. ... Brette F, Orchard C. T-tubule function in mammalian cardiac myocytes. Circ Res. 2003; 92: 1182-1192. ...http://circres.ahajournals.org/content/106/10/1582
Activation of Distinct cAMP-Dependent and cGMP-Dependent Pathways by Nitric Oxide in Cardiac Myocytes | Circulation Research
Cardiac Myocyte Isolation. Single cardiac myocytes were isolated via a previously described technique with minor modifications. ... Noncardiac myocytes comprised ≪1% of cells prepared in this manner. Cardiac myocyte viability was typically 70% to 80%. All ... In frog cardiac myocytes and in human atrial cells, cGMP has been shown to decrease Ca2+ influx through L-type channels by the ... Activation of Distinct cAMP-Dependent and cGMP-Dependent Pathways by Nitric Oxide in Cardiac Myocytes. Martin G. Vila-Petroff, ...http://circres.ahajournals.org/content/84/9/1020
Stable clones of HL-1 myocyte cell lines - Quicktech
Cardiac structure and functionare commonly studied using primary culture of neonatal and adult cardiac myocyte. However, their ... Established from a mouse atrial myocyte tumour, HL-1 cells share similar characteristics with primary cultures of cardiac ... Stable clones of HL-1 myocyte cell lines. Isolated homogeneous and stable clones of HL-1 cell lines ... Clones 3 and 6 appear to be most promising for cardiac research. These cells exhibit resting membrane potentials comparable to ...http://quicktech.imperialinnovations.co.uk/i/Cell_Lines/HL-1_clones.html
c-kit Is Required for Cardiomyocyte Terminal Differentiation | Circulation Research
Poolman RA, Li J-M, Durand B, Brooks G. Altered expression of cell cycle proteins and prolonged duration of cardiac myocyte ... Comparison of mitosis in binucleated and mononucleated newt cardiac myocytes. Anat Rec. 1998; 251: 245-255. ... Anversa P, Kajstura J, Leri A, Boli R. Life and death of cardiac stem cells: a paradigm shift in cardiac biology. Circulation. ... In W/Wv mice, c-kit dysfunction also resulted in an ≈14-fold decrease (P,0.01) in the number of c-kit+/GATA4+ cardiac ...http://circres.ahajournals.org/content/102/6/677
Establishment of β-Adrenergic Modulation of L-Type Ca2+ Current in the Early Stages of Cardiomyocyte Development | Circulation...
Ultrastructure and cell-cell coupling of cardiac myocytes differentiating in embryonic stem cell cultures. Cell Motil ... and cell-cell junctions similar to those observed in cardiac myocytes developing in vivo.13 The electrophysiological ... on membrane currents of isolated guinea-pig cardiac myocytes. Pflugers Arch. 1988;412:248-252. ... Development of cardiac innervation, ventricular fibrillation, and sudden infant death syndrome. Am J Physiol. 1992;263:H1514- ...http://circres.ahajournals.org/content/84/2/136
Poolman RA, Li JM, Durand B, Brooks G. Altered expression of cell cycle proteins and prolonged duration of cardiac myocyte ... Brooks G, Poolman RA, Li JM. Arresting developments in the cardiac myocyte cell cycle: role of cyclin-dependent kinase ... Goldman B, Mach A, Wurzel J. Epidermal growth factor promotes a cardiomyoblastic phenotype in human fetal cardiac myocytes. Exp ... Li F, Wang X, Capasso JM, Gerdes AM. Rapid transition of cardiac myocytes from hyperplasia to hypertrophy during postnatal ...http://ajpcell.physiology.org/content/296/4/C735
Protocols and Video Articles Authored by Lijun Liu
Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy', 'A Model for ... Subunits of Na(+)/K(+)-ATPase were detected by immunoblot analysis in caveolae isolated from cardiac myocytes, cardiac ... and hypertrophic growth in cardiac myocytes. 3) Unlike PP2, pNaKtide does not affect IGF-induced ERK activation in cardiac ... but not in KO myocytes. Ouabain-induced activations of ERK 1/2 were noted in Wt myocytes, but not in KO myocytes; however, ...https://www.jove.com/author/Lijun_Liu
Abstract 191: Phosphodiesterase 9a Modulates Cardiomyocyte Natriuretic Peptide-Stimulated cGMP Pool and Cardiac Hypertrophy |...
PDE9a gene expression is observed in rat neonatal cardiac myocytes (RNCM) and adult mouse myocytes. Protein expression was ... Cardiac hypertrophy in response to pathologic stress is a major contributor to heart disease. Many studies have revealed that ... Abstract 191: Phosphodiesterase 9a Modulates Cardiomyocyte Natriuretic Peptide-Stimulated cGMP Pool and Cardiac Hypertrophy. ... Together, these data identify PDE9a as a novel regulator of myocyte cGMP and hypertrophy that impacts a different ...http://circres.ahajournals.org/content/113/Suppl_1/A191
Letter by Sluijter et al Regarding Article, "Human Cardiac Stem Cell Differentiation Is Regulated by a Mircrine Mechanism" |...
Hosoda et al also observed that Sox6 is a direct target of the myocyte-specific miR-499; moreover, via RNAi knockdown of Sox6, ... These miRNAs become highly enriched, together with miR-499, on cardiac differentiation.2 Because the c-kit-positive rat cardiac ... Human cardiac stem cell differentiation is regulated by a mircrine mechanism. Circulation. 2011;123:1287-1296. ... Letter by Sluijter et al Regarding Article, "Human Cardiac Stem Cell Differentiation Is Regulated by a Mircrine Mechanism". ...http://circ.ahajournals.org/content/124/17/e456
Intracellular calcium homeostasis in cardiac myocytes. | Circulation
Calcium homeostasis in cardiac myocytes results from the integrated function of transsarcolemmal Ca2+ influx and efflux ... It may be anticipated that a rapid increase in our understanding of the pathophysiology of Ca2+ homeostasis in cardiac myocytes ... Intracellular calcium homeostasis in cardiac myocytes.. W H Barry, J H Bridge ...http://circ.ahajournals.org/content/87/6/1806
WISDEM seminar: Signalling to gene expression in cardiac myocytes
Seminar by Professor Angela Clerk on 'Signalling to gene expression in cardiac myocytes'. ... Signalling to gene expression in cardiac myocytes'. Abstract. Cardiomyocytes, the terminally-differentiated contractile cells ...https://warwick.ac.uk/insite/events/events/wisdem_seminar_120112/
Signaling pathways in cardiac myocyte hypertrophy.
... involved in mediating cardiac myocyte hypertrophy, and has enabled the molecular dissection of the pathways involved in signal ... Previous Document: Phosphatidic acid: a potential signal transducer for cardiac hypertrophy.. Next Document: Molecular ... mechanisms of angiotensin II in modulating cardiac function: intracardiac effects and sign.... ...http://www.biomedsearch.com/nih/Signaling-pathways-in-cardiac-myocyte/9405163.html
NASA - Conversion of Adipogenic Mesenchymal Stem Cells into Mature Cardiac Myocytes
Cardiac Myocytes flies the cardiac progenitor cells (CPCs) in a Techshot Bioreactor on the ISS and evaluates myocyte maturation ... Conversion of Adipogenic Mesenchymal Stem Cells into Mature Cardiac Myocytes (Cardiac Myocytes) - 09.27.17. Overview , ... Ground studies for Conversion of Adipogenic Mesenchymal Stem Cells into Mature Cardiac Myocytes (Cardiac Myocytes) are complete ... Conversion of Adipogenic Mesenchymal Stem Cells into Mature Cardiac Myocytes (Cardiac Myocytes) uses the microgravity ...https://www.nasa.gov/mission_pages/station/research/experiments/2404.html
NIOSHTIC-2 Publications Search - 00205787 - Automated surface area measurement of cultured cardiac myocytes.
An automated method measuring cardiac myocyte surface area was described and used as an index of cell growth. Hearts from 2 to ... An automated method measuring cardiac myocyte surface area was described and used as an index of cell growth. Hearts from 2 to ... Areas of individual myocytes varied widely, with the distribution skewed toward larger cells. The standard deviation increased ...https://www.cdc.gov/niosh/nioshtic-2/00205787.html
Subcellular Targeting of PDE4 in Cardiac Myocytes and Generation of Signaling Compartments | SpringerLink
... three decades of pharmacological data have clearly implicated PDE3 in cardiac function. Conversely, much less was known about ... McCahill A, Campbell L, McSorley T, Sood A, Lynch MJ, Li X, Yan C, Baillie GS, Houslay MD (2008a) In cardiac myocytes, cAMP ... In this chapter, the molecular properties and function of PDE4s in the different compartments of the cardiac myocytes will be ... Bers DM (2008) Calcium cycling and signaling in cardiac myocytes. Annu Rev Physiol 70:23-49. doi: 10.1146/annurev.physiol. ...https://link.springer.com/chapter/10.1007/978-3-319-54579-0_8
Cardiac myocytes, light micrograph - Stock Image - C038/4789 - Science Photo Library
Light micrograph of cardiac myocytes stained with a specific method for lipofuscin (Long Ziehl-Neelsen). The lipofuscin ... Light micrograph of cardiac myocytes stained with a specific method for lipofuscin (Long Ziehl-Neelsen). The lipofuscin ...https://www.sciencephoto.com/media/911517/view/cardiac-myocytes-light-micrograph
Antiapoptotic Actions of Methyl Gallate on Neonatal Rat Cardiac Myocytes Exposed to H2O2
... in cardiac myocyte medium (CMM) supplemented with 5% FBS, 1% cardiac myocyte growth supplement, and 1% penicillin/streptomycin ... Antiapoptotic Actions of Methyl Gallate on Neonatal Rat Cardiac Myocytes Exposed to H2O2. Sandhya Khurana,1 Amanda ... S. A. Cook and P. A. Poole-Wilson, "Cardiac myocyte apoptosis," European Heart Journal, vol. 20, no. 22, pp. 1619-1629, 1999. ... M. T. Crow, K. Mani, Y.-J. Nam, and R. N. Kitsis, "The mitochondrial death pathway and cardiac myocyte apoptosis," Circulation ...https://www.hindawi.com/journals/omcl/2014/657512/