Through a series of knockout experiments in mice in which one or several of the TR isoforms were delet … ... Thyroid hormone action: insight from transgenic mouse models J Investig Med. 2003 Jul;51(4):215-20. doi: 10.1136/jim-51-04-22. ... Through a series of knockout experiments in mice in which one or several of the TR isoforms were deleted, it has been ... These mice displayed a complete hypothyroid phenotype, demonstrating that the unliganded TR mediates the effect of ...
8 and in transgenic mouse models containing either a genomic fragment of human HD exon 1 (ref. 9) or an expanded repeat ... we examined instability of the HD CAG repeat by crossing transgenic mice carrying exon 1 of human HD (ref. 16) with Msh2-/- ... inserted into the endogenous mouse gene Hdh (ref. 10). With increasing repeat number, the protein changes conformation and ... Msh2 deficiency prevents in vivo somatic instability of the CAG repeat in Huntington disease transgenic mice. *Kevin Manley1, ...
We recently described transgenic mice that express point-mutant human K18 (Ku, N.-O., S. Michie, R.G. Oshima, and M.B. Omary. ... Susceptibility to hepatotoxicity in transgenic mice that express a dominant-negative human keratin 18 mutant.. N O Ku, S A ... Here we show that mutant K18 expressing transgenic mice are highly susceptible to hepatotoxicity after acute administration of ... The predisposition to hepatotoxicity results directly from the keratin mutation since nontransgenic or transgenic mice that ...
I recommend considering Mouse Transgenic Models and Advanced Mouse Transgenic Models coordinated by Dr. Lucy Liaw of Maine ... courseworkfeaturedMMCRImouse modelsmouse transgenics. February 2021 contents *Routine Examination: Maintaining Good Mental ... Tag Archives: mouse transgenics articles, Community, Events for Students, Summer 2016, Techniques ... Notes from the North - MMCRI mouse transgenic expertise from the comfort of your own bench!. July 26, 2016. Jess Davis-Knowlton ...
For this purpose, a transgenic mouse line expressing a green fluorecescent protein (GFP) under the control of the GAD65 (GAD: ... Characterisation of GABAergic interneurons in the amygdala of GAD65-GFP transgenic mice. ...
Transgenic Mice Expressing Porcine Prion Protein Resistant to Classical Scrapie but Susceptible to Sheep Bovine Spongiform ... Transgenic Mice Expressing Porcine Prion Protein Resistant to Classical Scrapie but Susceptible to Sheep Bovine Spongiform ... Brain atypical proteinase K-resistant prion protein (PrPres) of porcine PrP transgenic mice infected with an atypical scrapie ( ... before passage in the porcine mouse model are shown for comparison. MW, molecular mass in kilodaltons. C) Triangular plot of ...
Screening of transgenic mice. Transgenic mice were screened via PCR similar to the method described by Busler and Li (1996). ... GIN transgenic mice: EGFP expression and interneuronal subtypes. Hippocampal EGFP-expressing cells of GIN transgenic line were ... Founder mice were bred to wild-type FVB mice (Harlan Sprague Dawley, Indianapolis, IN), and resultant F1 heterozygotic mice ... Creation of transgenic mice. pGAD67-EGFP was digested withSfiI, and the transgene DNA subsequently gel-purified. Pronuclear ...
In this study, transgenic and nontransgenic control mice were generated from C57B6/SJL N2 generation Tg2576 mice backcrossed to ... In addition, transgenic mice overexpressing the mutant genes linked to familial AD produce high levels of Aβ, exhibit some of ... 1997a) APPSw transgenic mice develop age-related Aβ deposits and neuropil abnormalities, but no neuronal loss in CA1. J ... 2001) The curry spice curcumin reduces oxidative damage and amyloid pathology in an Alzheimer transgenic mouse. J Neurosci 21: ...
... by the production of model mice harbouring human growth hormone, and thwe identification of the transgenic mice. ... Transgenic mouse technology enables the study of gene regulation, embryology and development. The objective of this research ... The introduction of foreign genetic material into mouse genomes by microinjection is an important tool in studying the ... was the optimization of transgenic manipulation in the laboratory, ...
... wild type or transgenic) (table 1, model A, p , 0.001). Brain tissues of transgenic mice and all tissues of wild-type mice ... more in transgenic than in wild-type mice; 2.47% more than in the hippocampus and 10.82% more than in blood for transgenic mice ... for transgenic mice). In transgenic mice, these variables had a higher impact on the percentage of mtDNA copies with deletions ... both being more pronounced in transgenic mice) was observed. Pontine nuclei, the affected tissue in transgenic mice, displayed ...
To investigate the possible effects of the expression of this tightly regulated gene in vivo, we produced transgenic mice in ... LYMPHOID ABNORMALITIES IN CD40 LIGAND TRANSGENIC MICE SUGGEST THE NEED FOR TIGHT REGULATION IN GENE THERAPY APPROACHES TO HYPER ... Lymphoid abnormalities in CD40 ligand transgenic mice suggest the need for tight regulation in gene therapy approaches to hyper ... Overexpression in mature T cells is compatible with life, but in one-third of the cases, mice developed atypical lymphoid ...
This study was carried out in 6 and 12 months old transgenic PLP-α-syn and WT male and female mice. Heart rate variability (HRV ... QT and QTc intervals were not significantly modified in PLP-α-syn mice. An impaired HRV was observed at 12 months of age in PLP ... QT and QTc intervals were not significantly modified in PLP-α-syn mice. An impaired HRV was observed at 12 months of age in PLP ... BRS was significantly changed in PLP-α-syn mice and was age-dependent. ...
Celastrol Blocks Neuronal Cell Death and Extends Life in Transgenic Mouse Model of Amyotrophic Lateral Sclerosis Special ... Celastrol Blocks Neuronal Cell Death and Extends Life in Transgenic Mouse Model of Amyotrophic Lateral Sclerosis. Neurodegener ... and GFAP immunoreactivity in the lumbar spinal cord sections of celastrol-treated G93A mice compared to untreated G93A mice. ... Survival of celastrol-treated G93A mice increased by 9.4% and 13% for 2 mg/kg/day and 8 mg/kg/day doses, respectively. Cell ...
... previously developed a transgenic mouse model in which the SV40 T antigen induces RPE tumor formation. This transgenic mouse ... Relevant mouse models of human uveal melanoma still remain to be developed. We have studied the transgenic mouse strain, Tyrp-1 ... We studied normal CB6 mice (WT) and transgenic CB6 Tyrp-1-TAg mice [10] between the ages of P15 and three months. ... We performed semi-quantitative RT-PCR assays on total RNA from the eyes of normal mice (WT) and transgenic mice (Tyrp-1-TAg). ...
In the present study, we wanted to examine whether transgenic HD mice display altered sensitivity to excitotoxicity in the ... In the present study, we wanted to examine whether transgenic HD mice display altered sensitivity to excitotoxicity in the ... We demonstrate that the R6/2 transgenic mouse model expressing a short fragment of mutant HTT displays hypothalamic ... We demonstrate that the R6/2 transgenic mouse model expressing a short fragment of mutant HTT displays hypothalamic ...
H-single and H/L-double transgenic mice displayed comparable high amounts of IgG3 cryoglobulins, but only H/L-double transgenic ... To address this question, we have generated transgenic mice expressing only the H chain gene or both H and L chain genes of the ... A transgenic mouse model of autoimmune glomerulonephritis and necrotizing arteritis associated with cryoglobulinemia. In: The ... A transgenic mouse model of autoimmune glomerulonephritis and necrotizing arteritis associated with cryoglobulinemia. ...
Arnold, B.; Messerle, Martin; Jatsch, L.; Kühlbeck, G. and Koszinowski, Ulrich H. (1990): Transgenic mice expressing a soluble ... For this purpose, transgenic (H-2b x H-2d)F1 mice were constructed that carry integrated copies of a modified H-2Kk gene ... In contrast, only transgenic mice failed to generate a cytolytic T-cell response to soluble H-2Kk antigen expressed by ... Despite the presence of H-2Kk antigen, these mice were still able to generate an H-2Kk-specific T-cell response. This response ...
HLA-A*11:01 transgenic mice immunized with LO protein plus GLA-SE were protected compared with control mice inoculated with PBS ... 01 transgenic mice. These multi-epitope vaccines increased memory CD8+ T cells that produced IFN-γ and protected mice against ... Adjuvanted multi-epitope vaccines protect HLA-A*11:01 transgenic mice against Toxoplasma gondii. ... Adjuvanted multi-epitope vaccines protect HLA-A*11:01 transgenic mice against Toxoplasma gondii. ...
To broadly characterize OLM cells, we used the Sst‐Cre and the BAC transgenic Htr3a‐Cre mouse lines and separately analysed ... To broadly characterize OLM cells, we used the Sst‐Cre and the BAC transgenic Htr3a‐Cre mouse lines and separately analysed ... Single‐cell RNA‐Seq characterization of anatomically identified OLM interneurons in different transgenic mouse lines ... Seq characterization of anatomically identified OLM interneurons in different transgenic mouse lines. European Journal of ...
MT-Exendin transgenic mice do not produce antibodies to ssDNA or dsDNA.. To determine whether MT-Exendin transgenic mice show ... Mice.. MT-Exendin transgenic mice express the lizard proexendin-4 cDNA under the control of an inducible mouse metallothionein- ... Detection of antibodies to Ex-4, ENTP, GLP-1, or proexendin-4 in mouse serum.. Control or MT-Exendin transgenic mouse serum was ... All studies were carried out using male and female mice from both lines of MT-Exendin transgenic mice, unless otherwise ...
Human angiotensin-converting enzyme 2 transgenic mice infected with SARS-CoV-2 develop severe and fatal respiratory disease. ... Human angiotensin-converting enzyme 2 transgenic mice infected with SARS-CoV-2 develop severe and fatal respiratory disease. ... Male mice that succumbed to disease had higher levels of inflammatory transcripts compared with female mice. To our knowledge, ... Mice are refractory to SARS-CoV-2 infection owing to low-affinity binding to the murine angiotensin-converting enzyme 2 (ACE2) ...
a: Wild-type mouse (group 1). COX2 expression limited to scattered dermal leukocytes, ×40. b: Untreated HPV16-transgenic mouse ... transgenic mice. Materials and Methods: Six-week-old FVB/n mice were supplemented with rutin or curcumin for 24 weeks: HPV16−/− ... HPV-transgenic mouse models: tools for studying the cancer-associated immune response. Virus Research 235: 49-57, 2017. ... Celecoxib promotes degranulation of CD8+ T-cells in HPV-induced lesions of K14-HPV16 transgenic mice. Life Sciences 157: 67-73 ...
Kollias G., Grosveld F. 1992; The study of gene regulation in transgenic mice. In Transgenic Animals pp 79-98 Grosveld F., ... Kim C. M., Koihe K., Saito I., Miyamaura T., Jay G. 1991; HBx gene of hepatitis B virus induces liver cancer in transgenic mice ... Vogel J., Hinrichs S. H., Napolitano L. A., Ngo L., Jay G. 1991; Liver cancer in transgenic mice carrying the human ... Mehtali M., Munschy M., Ali-Hadji D, Kieny M. P. 1992; A novel transgenic mouse model for the in vivo evaluation of antihuman ...
Much of our work employs transgenic mice which over-produce Abeta peptides, molecules that are widely believed to play a ... Home » Database » Modified intrinsic excitability in transgenic mouse models of progressive beta-amyloidopathy ... In recent years, work with such mice, by ourselves and others has begun to reveal significant disturbances to synchronous ... and their presence seems to parallel Abeta burden since they arise earlier in the more aggressive PSAPP double transgenic line ...
To elucidate the role of T1 in vivo, we generated T1-deficient mice and a T1-transgenic strain which secretes soluble T1-Fc ... However, induction of all other immune parameters was normal and both T1-deficient and T1-Fc-transgenic mice were able to clear ... T1-deficient and T1-Fc-transgenic mice develop a normal protective Th2-type immune response following infection with ... a decrease in IL-5 production by lung lymphocytes was detected in both T1-deficient and T1-Fc-transgenic mice compared to ...
p16 expression can be induced in a regulated fashion in the doubly transgenic mice by administering antibiotic doxycycline or ... TetOp16 mice can be mated to mice carrying rtTA transgenes in the tissue of interest. ... TetOp16 transgenic mice allow for the inducible expression of p16INK4a. In these transgenic mice, the nucleic acid sequence ... TetOp16 transgenic mice offer a way to selectively manipulate cell proliferation by allowing for temporal and spatial ...
... mouse models: xenotransplantation of primary Ph-positive CML cells into immunodeficie ... recent studies have reported successful establishment of making the CMLphenotype in transgenic mice. The transgenic CML mouse ... recent studies have reported successful establishment of making the CMLphenotype in transgenic mice. The transgenic CML mouse ... This article reviews the history of BCR/ABL-expressing CML mouse models and the recent findings in the transgenic mice with the ...
In human amyloid precursor protein (hAPP) transgenic mice, co-expression of hTau-A152T enhances risk of early death and ... Comparing mice with regulatable expression of hTau-A152T or wild-type hTau (hTau-WT), we find age-dependent neuronal loss, ... hTau-A152T mice have higher hTau protein/mRNA ratios in brain, suggesting that A152T increases production or decreases ... Despite their functional abnormalities, aging hTau-A152T mice show no evidence for accumulation of insoluble tau aggregates, ...
Jeet V, Russell PJ, Khatri A. Modeling prostate cancer: a perspective on transgenic mouse models. Cancer and Metastasis Reviews ... Animal models, especially, transgenic immunocompetent mouse models, have proven to be the best ally in this respect. A series ... Animal models, especially, transgenic immunocompetent mouse models, have proven to be the best ally in this respect. A series ... Animal models, especially, transgenic immunocompetent mouse models, have proven to be the best ally in this respect. A series ...