In vitro Analysis for Macrophage Binding and Pro-inflammatory Responses to Candida albicans -BIO-PROTOCOL
In particular, methods involved in fluorescent labeling of live C. albicans, quantification of macrophage binding of the ... Dectin-1 is the major macrophage receptor that is known to recognize fungal Beta-glucans leading to induction of various immune ... Macrophage recognition of Candida albicans (C. albicans) is facilitated by pattern recognition receptors that interact with the ... Macrophage binding and pro-inflammatory assays Notes: * Extraction of macrophages is done in a hood within animal facility. ...http://www.bio-protocol.org/e1123
Increased susceptibility to activation and increased uptake of low density lipoprotein by cholesterol-loaded macrophages. |...
... mouse peritoneal macrophages and human monocyte-derived macrophages. In LPS-stimulated macrophages, high density lipoprotein- ... Cellular cholesterol synthesis, however, was increased by 25% in the activated macrophages. Macrophage activation, measured as ... Increased susceptibility to activation and increased uptake of low density lipoprotein by cholesterol-loaded macrophages.. J ... Macrophages (J774A.1) that were activated with either lipopolysaccharide (LPS), zymosan, or phorbol ester demonstrated a 30-35 ...http://atvb.ahajournals.org/content/12/6/745
Human bronchoalveolar macrophage heterogeneity demonstrated by histochemistry, surface markers and phagocytosis. - Semantic...
Such heterogeneity may reflect different stages of macrophage maturation or the existence of macrophage subpopulations with ... and two anti-monocyte/macrophage MoAb (UCHMI and RFD2); and (4) simultaneous phagocytosis of C. guillermondii and staining with ... Thus only a proportion of Ia positive AM expressed monocyte/macrophage antigens and were phagocytic. ... Human alveolar macrophages (AM) were obtained by bronchoalveolar lavage from 18 patients with a variety of conditions. For each ...https://www.semanticscholar.org/paper/Human-bronchoalveolar-macrophage-heterogeneity-dem-Gant-Hamblin/072e73426b9a70a1e605dbbd95e88d5da41696f4
A Salmonella protein causes macrophage cell death by inducing autophagy | JCB
SipB could be readily detected in macrophages infected with wild-type (A and B), but not in macrophages infected with the sipD ... 2; unpublished data), presumably because transfected macrophages detached from the dish. These macrophages exhibited obvious ... macrophages. More importantly, expression of SipB in caspase-1−/− macrophages resulted in their death. The involvement of SipB ... macrophages. We propose from these analyses that Salmonella-induced macrophage cell killing is the result of autophagy-mediated ...http://jcb.rupress.org/content/163/5/1123
MicroRNAs in Immune Response and Macrophage Polarization | Arteriosclerosis, Thrombosis, and Vascular Biology
miRNA in Macrophage Polarization. Macrophages display remarkable plasticity, with the ability to undergo dynamic transition ... macrophages. The miR-155 target, C/EBP-β, seems to play a key role in this process.76 C/EBP-β, a hallmark of M2 macrophages ... 76 Akt1 ablation in macrophages gave rise to an M1 macrophage phenotype, and Akt2 ablation resulted in an M2 phenotype.76 Akt2 ... 66 The classical M2 macrophage markers in mice, such as arginase-1, YM-1, and FIZZ1, are not induced in human macrophages even ...http://atvb.ahajournals.org/content/33/2/170.full
Thiol Oxidative Stress Induced by Metabolic Disorders Amplifies Macrophage Chemotactic Responses and Accelerates Atherogenesis...
Macrophage Eh correlated with the macrophage content in both atherosclerotic (r2=0.346, P=0.004) and renal lesions (r2=0.480, P ... The macrophage Eh was a strong predictor of macrophage chemotaxis (r2=0.554, P,0.001). ... Macrophages are continually recruited into atherosclerotic lesions, and macrophage accumulation appears to increase in ... Our data underscore the critical role of the macrophage glutathione redox state in regulating macrophage chemotaxis and ...http://atvb.ahajournals.org/content/29/11/1779
Glass, C.K. (2001) Potential roles of the peroxisome proliferator-activated receptorγ in macrophage biology and atherosclerosis...
Potential roles of the peroxisome proliferator-activated receptorγ in macrophage biology and atherosclerosis. Journal of Endo- ... Glass, C.K. (2001) Potential roles of the peroxisome proliferator-activated receptorγ in macrophage biology and atherosclerosis ...http://www.scirp.org/reference/ReferencesPapers.aspx?ReferenceID=520967
Early Macrophage Recruitment and Alternative Activation Are Critical for the Later Development of Hypoxia-Induced Pulmonary...
Hypoxia Induces Alternative Activation of Macrophages: Suppressive Effect of Heme Oxygenase-1. The observation that macrophage ... Heme oxygenase-1 induction promotes the expression of interleukin (IL)-10 in alveolar macrophages. Alveolar macrophages were ... Interestingly, a striking alteration of macrophage morphology in cytospin preparations of BALF-isolated macrophages was ... macrophages.11,12 Classically activated macrophages are effector phagocytes activated by interferon-γ and tumor necrosis factor ...http://circ.ahajournals.org/content/123/18/1986
TH2 cytokines and allergic challenge induce Ym1 expression in macrophages by a STAT6-dependent mechanism. - Oxford Neuroscience
Macrophage expression of Ym1 was highly induced in vivo by an IL-4- and STAT6-dependent mechanism during the evolution of ... Microarray analysis of the transcriptional response of mouse peritoneal macrophages to the T(H)2 cytokine interleukin-4 (IL-4) ... Molecular characterization of the Ym1 promoter in transfected epithelial and macrophage cell lines revealed the presence of ... supporting the biological relevance of the IL-4-dependent pathway characterized ex vivo in peritoneal macrophages. These ...https://www.neuroscience.ox.ac.uk/publications/20623
Generation of anti-inflammatory macrophages for implants and regenerative medicine using self-standing release systems with a...
Macrophages are highly plastic cells that define the tissue reactions on the implanted material. Local control of macrophage ... Gelatin; Cytokine; Macrophage phenotype control; Controlled release; Wound healing. Schools/Departments:. University of ... However, in the absence of M2Ct in the medium macrophages underwent rapid pro-inflammatory re-programming upon IFNg stimulation ... Herein, we describe the development of a cytokine cocktail (M2Ct) that induces stable M2-like macrophage phenotype with ...http://eprints.nottingham.ac.uk/40797/
Immortalized Murine Macrophage Cell Line as a Model for Macrophage Polarization into Classically Activated M(IFNÎ³+LPS) or...
Immortalized Murine Macrophage Cell Line as a Model for Macrophage Polarization into Classically Activated M(IFNÎ³+LPS) or ... Immortalized Murine Macrophage Cell Line as a Model for Macrophage Polarization into Classically Activated M(IFNÃ Â³+LPS) or ... Immortalized Murine Macrophage Cell Line as a Model for Macrophage Polarization into Classically Activated M(Ifnγ+Lps) or ... Objective: Macrophages (MÃ Â ) represent a link between the innate and adaptive arms of the immune system. Generally, MÃ Â are ...https://www.omicsonline.org/peer-reviewed/immortalized-murine-macrophage-cell-line-as-a-model-for-macrophage-polarization-into-classically-activated-mifnlps-or-alternativel-52356.html
Efferocytosis Signaling in the Regulation of Macrophage Inflammatory Responses | The Journal of Immunology
Efferocytosis Signaling in the Regulation of Macrophage Inflammatory Responses. Michael R. Elliott, Kyle M. Koster, Patrick S. ... Efferocytosis Signaling in the Regulation of Macrophage Inflammatory Responses. Michael R. Elliott, Kyle M. Koster, Patrick S. ... Efferocytosis Signaling in the Regulation of Macrophage Inflammatory Responses. Michael R. Elliott, Kyle M. Koster and Patrick ... Efferocytosis Signaling in the Regulation of Macrophage Inflammatory Responses Message Subject (Your Name) has forwarded a page ...http://www.jimmunol.org/content/198/4/1387
Lymphokine enhances the expression and synthesis of Ia antigens on cultured mouse peritoneal macrophages. | JEM
Lymphokine enhances the expression and synthesis of Ia antigens on cultured mouse peritoneal macrophages.. R M Steinman, N ... Lymphokine enhances the expression and synthesis of Ia antigens on cultured mouse peritoneal macrophages. ... cruzi-immune spleen cells enhanced the expression of Ia antigens on proteose-peptone-elicited mouse peritoneal macrophages (M ...http://jem.rupress.org/content/152/5/1248
Adipose Fatty Acid Binding Protein Promotes Saturated Fatty Acid-Induced Macrophage Cell Death through Enhancing Ceramide...
Immortalized macrophage cell lines were established from A-FABP-deficient (A-FABP−/− macrophages) or WT mice (WT macrophages) ( ... For analyzing macrophage phenotype, macrophage cell lines or M-BMMs were surface stained for 15-30 min at 4°C in 1% BSA PBS ... Primary macrophages were differentiated from either M-CSF-induced bone marrow-derived macrophages (BMMs) (M-BMMs) or GM-CSF- ... Analysis of macrophage morphology by electron microscopy. Control or sFA-treated macrophage cells were pelleted and fixed in ...http://www.jimmunol.org/content/198/2/798
Genetic background affects the expansion of macrophage subsets in the lungs of Mycobacterium tuberculosis-infected hosts. -...
Our findings show a higher ratio of macrophages to M2 macrophages in the lungs of chronically infected C57BL/6 mice compared ... However, there was no difference in the functional ability of M1 and M2 macrophages for the two strains in vitro. In vivo, a ... We hypothesized that infected C57BL/6 mice would exhibit a higher frequency and function of M1 macrophages than infected BALB/c ... The contribution of our findings is that although M1 macrophage is an effector leucocyte with microbicidal machinery, its ...https://www.semanticscholar.org/paper/Genetic-background-affects-the-expansion-of-macrop-Bertolini-Souza/33d5d9d4835966ee79be57dd902912680fbcad46
Perivascular Macrophages Are the Primary Cell Type Productively Infected by Simian Immunodeficiency Virus in the Brains of...
CNS macrophages are the target of SIV infection in animals with SIVE. CNS macrophages (CD11b, green), astrocytes (GFAP, yellow ... In this study we immunophenotypically differentiate subpopulations of brain macrophages into perivascular macrophages and ... and perivascular macrophages (15)(16)(18)(19)(20)(21)(22). These brain macrophages are distinguished by their anatomic location ... perivascular macrophages) in normal brain and on macrophages within meninges and the choroid plexus. CD14 and CD45 were readily ...http://jem.rupress.org/content/193/8/905
The polysulphated polyxylan Hoe/Bay-946 inhibits HIV replication on human monocytes/macrophages - Publikationsserver der...
Due to the important role of monocytes/macrophages in the pathogenesis of AIDS, potential drugs with anti-HIV activity in ... Due to the important role of monocytes/macrophages in the pathogenesis of AIDS, potential drugs with anti-HIV activity in ... The polysulphated polyxylan Hoe/Bay-946 inhibits HIV replication on human monocytes/macrophages ... For testing the efficacy of antiviral substances, monocytes/macrophages from peripheral blood were infected, respectively, with ...https://epub.uni-regensburg.de/14253/
Natural, genetically determined resistance toward influenza virus in hemopoietic mouse chimeras. Role of mononuclear phagocytes...
49 chimeras were tested individually, both of resistance of their macrophages to in vitro challenge with M-TUR (a strain of ... Cultivated Kupffer cells and peritoneal macrophages harvested from chimeric mice expressed the resistance phenotype of the bone ... to lethal challenge by a number of myxoviruses were used to test whether macrophage resistance (as assessed in vitro) and ... avian influenza virus A/Turkey/England/63 adapted to grow in cultured mouse peritoneal macrophages), and for resistance of the ...http://jem.rupress.org/content/150/1/117
Tumor-associated macrophage - Wikipedia
However, it remains unclear when M1 tumor-associated macrophages are transformed to M2 tumor-associated macrophages, but tumor ... Patients with more M1 tumor-associated macrophages displayed the opposite effect. . Macrophages are the most common cells in ... growth factors and immunosuppressive cytokines that convert trophic macrophages to tumor-associated macrophages (TAM). These ... Tumor-associated macrophages are mainly of the M2 phenotype, and seem to actively promote tumor growth. The designation of M1 ...https://en.wikipedia.org/wiki/Tumor-associated_macrophage
Induction of Immunity to Prostate Cancer Antigens: Results of a Clinical Trial of Vaccination with Irradiated Autologous...
4A) ⇓ . DTH site biopsies also disclosed ingress of macrophages (Fig. 4B) ⇓ and of natural killer cells (Fig. 4C) ⇓ . Extensive ... 2C) ⇓ . Macrophages were also detected (Fig. 2D) ⇓ . Neutrophils and eosinophils, usually present near vaccine cells in the ... 2E) ⇓ . CD3+ T-cells also appeared near DCs and macrophages recruited to vaccine sites. Frequent CD8+ T-cells were consistently ... Notably, DCs and macrophages were recruited for antigen processing by paracrine GM-CSF secretion at tumor vaccination sites ( ...http://cancerres.aacrjournals.org/content/59/20/5160
Isolation of Macrophages
This procedure usually gets you ,95% pure macrophages by non-specific esterase staining. If you want inflammatory macrophages, ... Isolation of Macrophages. Jay Mone' jmone at MARAUDER.MILLERSV.EDU Tue Jun 2 06:47:20 EST 1998 *Previous message: Isolation of ... Dave: To isolate resident peritoneal macrophages, kill the mouse, and immediately inject the peritoneum with about 5 mls of pre ...http://www.bio.net/bionet/mm/cellbiol/1998-June/008849.html
Hormonal Regulation of Uterine Macrophages
... Joan S. Hunt,1,2 Lance Miller,2 and Jeralyn Sue Platt1 ... Joan S. Hunt, Lance Miller, and Jeralyn Sue Platt, "Hormonal Regulation of Uterine Macrophages," Developmental Immunology, vol ...https://www.hindawi.com/journals/jir/1998/087527/cta/
killing macrophages by feeding
... brett at BORCIM.WUSTL.EDU brett at BORCIM.WUSTL.EDU Mon Apr 18 12:29:40 EST 1994 *Previous ... recently frank chisari from the scripps used carageenan to bump off the macrophages in vivo. i don't have the ref handy, but it ...http://www.bio.net/bionet/mm/immuno/1994-April/001268.html
macrophages | Gut
Pharmacological macrophage inhibition decreases metastasis formation in a genetic model of pancreatic cancer Heidi Griesmann, ... AODTU-007 Mertk expressing hepatic macrophages promote the resolution of inflammation in acute liver failure E Triantafyllou, O ... When antimicrobial peptides hit the wrong target: a novel link between tumour macrophages and cancer stem cells Giuseppe R ... Targeting of tumour-infiltrating macrophages via CCL2/CCR2 signalling as a therapeutic strategy against hepatocellular ...https://gut.bmj.com/keyword/macrophages?page=1
Ironing Out Macrophages | Science Signaling
As the serum concentration of iron decreases, iron accumulates in hepatic and splenic macrophages. Kim et al. report that the ... typhimurium) exploits this system to proliferate in macrophages. S. typhimurium infection causes the host to produce the ... thus reducing both the uptake of dietary iron and the release of iron into the bloodstream by the macrophages that recycle red ... processes influenced by ERRγ but also suggests that preventing or reversing the iron accumulation that occurs in macrophages ...http://stke.sciencemag.org/content/7/323/ec112?rss=1