*  how to confirm ligation is successful - Molecular Cloning
how to confirm ligation is successful - (Mar/01/2006 ). Hi.Could anyone tell me what method can i use in order to know my ... If its simple ligation and therefore expect positives you may need the plasmid so better make minipreps. You can check by ... I agree also, if its a complicated ligation and you expect to have few positive clones, then it is better to screen a lot of ... Anyway, you may need to sequence your plasmid at the end, depending what you need it for, to check correct ligation sites, etc ...
  http://www.protocol-online.org/biology-forums/posts/14130.html
*  Ligation issues: post #1
I was doing a three way ligation and it didnt work for a long time so taking a new approach with just a simple ligation and ... Ligation issues - posted in Molecular Cloning: Hi, I have been having problems with my cloning for the past 2 months. ... I was doing a three way ligation and it didnt work for a long time so taking a new approach with just a simple ligation and ... I tried 16C water bath over night and also I have tried to leave the ligation at RT for about and hr and then leaving in the ...
  http://www.protocol-online.org/forums/topic/7467-ligation-issues/
*  Oligo ligation to PCR product: post #1
Oligo ligation to PCR product - posted in Molecular Biology: I am using a 2 % Agarose / 1 M urea gel for separation and ... Oligo ligation to PCR product. Started by anonymous, Mar 22 2001 10:00 PM ...
  http://www.protocol-online.org/forums/topic/472-oligo-ligation-to-pcr-product/
*  Difference between revisions of "Corum:T4 Ligation" - OpenWetWare
Retrieved from "https://openwetware.org/mediawiki/index.php?title=Corum:T4_Ligation&oldid=621385" ...
  https://openwetware.org/wiki/?title=Corum:T4_Ligation&diff=621385&oldid=621384
*  Difference between revisions of "Jamesh008:consensus DNA ligation protocol" - OpenWetWare
Endy:DNA ligation using T4 DNA ligase -- Using T4 DNA Ligase Knight:DNA ligation using NEB Quick Ligation Kit -- 5min ligation ... Silver:Ligation -- A protocol using the Roche Kit. BE.109:DNA ligation -- A ligation protocol for classroom use in a laboratory ... If you are having trouble with your ligation, NEB offers FAQ's (Quick Ligation T4 DNA ligase) to help. ... Add 1 μL ligation buffer to the tube. Vortex buffer before pipetting to ensure that it is well-mixed. Remember that the buffer ...
  https://openwetware.org/wiki/?title=Jamesh008:consensus_DNA_ligation_protocol&diff=85298&oldid=84474
*  Unable to obtain colonies from PCR-insert + vector ligation - Molecular Cloning
Also tried ligation with a regular T4 DNA ligase (not the quick ligation kit), didn't work. I think maybe the problem is in the ... I tried getting rid of the PEG in the quick ligation mix using spin columns, and then transforming using the purified ligation ... Unable to obtain colonies from PCR-insert + vector ligation - (Jun/14/2006 ). I am using a pET-19b (5.5kb) and a 1.4kb insert, ... Last time, which is more than a year ago, I used the quick ligation kit (NEB), they suggested to heat shock cells and not ...
  http://www.protocol-online.org/biology-forums/posts/17293.html
*  Ligation problem: post #1
So we tried to CIP treat vector before ligation and that did not work either. Then we decid... ... Ligation problem - posted in Molecular Biology: Hi All, I am trying to insert a fragment in the .THT vector, which was cut with ... Incubation conditions: digestion was carried at 37C for 2 hours and ligation was done at 16C overnight. Right after ligation I ... Depending on the quantity of my ligation mixture, I will either run it on a gel and gel extract my ligated product or just ...
  http://www.protocol-online.org/forums/topic/30612-ligation-problem/
*  NEBNext Adaptor Ligation (E6080) | NEB
Home Protocols NEBNext Adaptor Ligation (E6080) NEBNext Adaptor Ligation (E6080). Protocol. *After completion of the End Repair ...
  https://www.neb.com/protocols/1/01/01/nebnext-adaptor-ligation-e6080
*  Ligation SOE PCR fragment help: post #1
However, mysteriously after ligation, transformation and test restriction the fragment is not 1000 bp, but either 400, 500, 750 ... Ligation SOE PCR fragment help - posted in Molecular Cloning: Hello everyone, I am trying to clone a 1000 bp fragment, obtained ... Cloning - Ligation problem Started by cahyaprihatna, 01 Apr 2015 ligation, problem, cloning * 3 replies ... Also tagged with one or more of these keywords: ligation, SOEing PCR, shorter fragments. Protocols and Techniques Forums → ...
  http://www.protocol-online.org/forums/topic/24123-ligation-soe-pcr-fragment-help/
*  Afl II ligations... any suggestions?
... Name fred at here.there.com Tue Mar 3 19:48:04 EST 1998 *Previous message: Tons of FREE ... I've tried ligation +/- PEG, high and low amounts of DNA, at 12 degrees C and room temp., and high and low ligase (400 and 2000 ...
  http://www.bio.net/bionet/mm/methods/1998-March/065113.html
*  In Gel Ligation - Molecular Biology - BioForum
I have come across a protocol for in gel ligation, where the RE digested insert and vector are run on a low melting point gel, ... Hi I am having real trouble getting a couple of ligations to work and have been searching around for any protocols that may ... the fragments are excised and melted and used directly in the ligation reaction Has anyone used this method before? Does it ... For trouble ligations I find that the Bioline Quick stick ligase work well. Also if I have a PCR product that has been ...
  http://www.protocol-online.org/forums/topic/5307-in-gel-ligation/
*  RNA Whole-Mount In situ Hybridisation Proximity Ligation Assay (rISH-PLA) (Myers and Scarlett labs) - Xenbase
6) The oocytes were then incubated with the ligation solution (20 ul of 5 x ligation stock, 77.5 ul of Sigma H2O (SH2O) and 2.5 ... 8) Excess unbound ligation solution was removed by washing 3 times for 5 min at RT on a rocker with 1ml of 1 x Wash Buffer A. ... RNA Whole-Mount In situ Hybridisation Proximity Ligation Assay (rISH-PLA) (Myers and Scarlett labs). From Xenbase ... Retrieved from "http://wiki.xenbase.org/xenwiki/index.php?title=RNA_Whole-Mount_In_situ_Hybridisation_Proximity_Ligation_Assay ...
  http://wiki.xenbase.org/xenwiki/index.php/RNA_Whole-Mount_In_situ_Hybridisation_Proximity_Ligation_Assay_
*  Triple ligation not working: post #1
I treated the vector backbone with CIP and purified . Tried to religate the inserts with CIP treated vector but no ligation was ... after ligation I used to get a band of around 6Kb but after transformation none of the colony screened show the presence of the ... Triple ligation not working - posted in Molecular Cloning: Hi all, I am trying to ligate insert 1 ( Nco1-Sac1, 2180 bp), ... I add ATP (2x) to the ligation, and incubate o/n at 8'C. -Be gentle when mixing. -include a controls with an intact vector as a ...
  http://www.protocol-online.org/forums/topic/12334-triple-ligation-not-working/
*  Endoscopic Ligation Compared with Sclerotherapy for the Treatment of Bleeding Esophageal Varices | Annals of Internal Medicine ...
Complications were less common in the ligation group: fewer patients in the ligation group had esophageal strictures (0 of 38 ... Endoscopic Ligation Compared with Sclerotherapy for Treatment of Esophageal Variceal Bleeding: A Meta-Analysis Annals of ... Endoscopic Ligation Compared with Sclerotherapy for the Treatment of Bleeding Esophageal Varices Loren Laine, MD; Hussein M. El ... Ligation is a viable alternative to sclerotherapy and may have some advantages as a treatment for bleeding esophageal varices. ...
  http://annals.org/aim/article-abstract/706486/endoscopic-ligation-compared-sclerotherapy-treatment-bleeding-esophageal-varices
*  Ligation
... ChenHA via methods%40net.bio.net (by hzhen from freeuk.com). Thu Jun 21 22:35:37 EST 2007 *Previous message: Ligation ... There are many variables in ligation (e.g. concentration and size of DNA, vector/insert ratio etc.), and this is the bit I'd ... Having good competent cells just makes life easier - I can do just a single ligation reaction for each construct (easier when I ... and I'd say having good competent cells is the main reason why I only need to do repeat ligation for a very small fraction of ...
  http://www.bio.net/bionet/mm/methods/2007-June/102307.html
*  Protein Ligation
... Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de Mon Jan 19 04:27:18 EST 1998 *Previous message: ...
  http://www.bio.net/bionet/mm/proteins/1998-January/006149.html
*  ligation/transformation
... schroeder at vzdmza.zdv.uni-mainz.de schroeder at vzdmza.zdv.uni-mainz.de Fri May 12 03:31:28 EST 1995 ... Ligations, when analyzed on a gel, seemed to work fine. When used for transformation only very few colonies are growing, which ...
  http://www.bio.net/bionet/mm/methods/1995-May/028290.html
*  Ligation failed
... newsnet customer customer at newsnet.com Sat May 6 09:19:09 EST 2006 *Previous message: Ligation failed ... Here is the ligation reaction: , , , , insert (12ng/ul) 2ul , , linearised vector (6ng/ul) 4ul , , 10X lig. buffer 1ul , , T4 ... Total DNA in ligation reaction is 48ng. , , insert:vector ratio is 1:1 in accordance to Roche T4 ligase information , , sheet ... Both the insert and the vector are large , enough that the intramolecular ligation has a higher chance than the , ...
  http://www.bio.net/bionet/mm/methods/2006-May/100520.html
*  RNA ligation
... Nick Theodorakis via methods%40net.bio.net (by nick.theodorakis from gmail.com). Wed Nov 18 20:54:25 EST 2009 * ...
  http://www.bio.net/bionet/mm/methods/2009-November/104406.html
*  ligation problem
... NJ njuni at poppy.ocn.ne.jp Wed May 23 00:30:21 EST 2001 *Previous message: ligation problem ... Control ligations include vector with ligase and no insert , , , , RESULT : NO CLONES , , I hope u have some ideas :) , , ... Checked purity and yield of vecotr and insert by running on gel and ligation , setup with excess insert over vecotr at 15C for ...
  http://www.bio.net/bionet/mm/methods/2001-May/088856.html
*  ligation problems. Help
... M K Bennett pamkb at mail.bris.ac.uk Tue Apr 25 06:48:24 EST 1995 *Previous message: ligation problems ... two-way ligation to work. My vector is : 6.5 kb, with two homologous repeats (the LTRs in pLXSN), and the : insert is 3.5 kb (b ... I have tried transforming ligations into : HB101 and XL1Blue, but keep obtaining colonies containing either : vector alone, or ...
  http://www.bio.net/bionet/mm/methods/1995-April/027625.html
*  EWTN.com - Bilateral tubal ligation
Tubal ligation is never morally permissible for the direct intention of preventing pregnancy. This is a moral principle which ... Automatically this third one should be by c section accompanied By a bilateral tubal ligation as future pregnancies may be ...
  http://www.ewtn.com/v/experts/showmessage.asp?number=633137&Pg=Forum10&Pgnu=1&recnu=21
*  Peptide Ligation | NEB
The IPL reaction allows ligation of a synthetic peptide or a protein with an N-terminal cysteine residue to the C-terminus of ... Peptide Ligation. Product Listing Application Overview The IPL reaction allows the ligation of a synthetic peptide or a protein ... The transitory ligation product then undergoes a spontaneous S-N acyl rearrangement from a thioester to a stable peptide bond. ... Home Applications Protein Analysis & Tools Protein Labeling Peptide Ligation ...
  https://www.neb.com/applications/protein-analysis-and-tools/protein-labeling/peptide-ligation
*  Vein ligation and stripping
This is the ligation part of the operation. The second step is where a thin flexible length of wire is inserted into the bottom ... Vein ligation and stripping This is the most common form of surgery for varicose veins. Vein stripping has been performed for a ... Recovery after vein ligation and stripping A surgical dressing or bandage is applied to the treated area. You will have to wear ... Advantages of vein ligation and stripping The main benefit from this surgery is that of removal of the offending vein. ...
  http://www.medic8.com/cosmetic-surgery/varicose-veins/vein-ligation-and-stripping.html
*  ligation of PCR products
... Jeffrey Lawrence jlawrenc at vms.cis.pitt.edu Mon Oct 7 11:46:55 EST 1996 *Previous message: ligation ... overhang will also interfere with ligations. this base can be removed with T4 DNA polymerase (and its ever-so-handy exo ...
  http://www.bio.net/bionet/mm/methods/1996-October/049939.html