Lentivirus. H9, human cell line, HIV-1 infected (C. Hanson). -. -. -. -. -. HIV-2. Lentivirus. H9, human cell line, HIV-2 ... Bovine Leukemia Virus DNA in Human Breast Tissue Gertrude Case Buehring. , Hua Min Shen, Hanne M. Jensen, K. Yeon Choi1, Dejun ... Bovine Leukemia Virus DNA in Human Breast Tissue. ... bovine leukemia virus; LTR, long terminal repeat (promoter ...

Chadwick, BJ (1995). "Nucleotide sequence analysis of Jembrana disease virus: a bovine lentivirus associated with an acute ... Bovine diseases, Lentiviruses, All stub articles, Infectious disease stubs). ... Bovine immunodeficiency virus G.E., Wilcox (8 March 1995). "Recent advances in the understanding of Jembrana disease". ... The Jembrana disease virus belongs to the genus Lentivirus, which include immunodeficiency viruses such as HIV. Instead of the ...

Isolation and characterization of sets of anti-L-fucose and anti-bovine serum albumin antibodies directed against a.... October ...

Small ruminant lentiviruses (SRLV) are members of the Retrovirus family comprising the closely related Visna/Maedi Virus (VMV) ... The influence of ovine MHC class II DRB1 alleles on immune response in bovine leukemia virus infection. Microbiol. Immunol. ... 1. Small Ruminant Lentiviruses (SRLVs). Lentiviruses are a subgroup of the Retrovirus family, which infect a wide range of ... This article belongs to the Special Issue Small Ruminant Lentiviruses). Download keyboard_arrow_down Download PDF Download PDF ...

Lentivirus-mediated overexpression of GATA3 significantly increased the number of GFAP/SOX2 double positive astrocytes and ... Lentivirus-mediated overexpression of GATA3 significantly increased the number of GFAP/SOX2 double positive astrocytes and ... fetal bovine serum (SRL, Catalog Number 0010), 1% astrocyte growth supplement (SRL, Catalog Number 1852) and 1% penicillin/ ... SP: generation of lentivirus, viral transduction. CP, PB, VM, TS, SW, SNB, INA, LN, and KB: collection of data. UF and CW: ...

After washing twice with PBS, cells were blocked for 20 min with 5% bovine serum albumin (BSA) in PBS. Primary antibody was ... At 6 days in vitro (DIV), neurons were transduced with recombinant lentiviruses expressing synucleins. Neurons were harvested ... HEK293T cells (ATCC) were maintained in DMEM with 1% penicillin and streptomycin and 10% bovine serum. For production of ... fetal bovine serum) was replaced with growth media (MEM containing 5 g/l glucose, 0.2 g/l NaHCO3, 0.1 g/l transferrin, 0.3 g/l ...

Shanghai, China). HUVECs were transfected with nontarget control lentivirus (lv-ctrl) or NOX2-targeted lentivirus (lv-NOX2) ... fetal bovine serum (Gibco, NY, USA), 100 U/mL penicillin (Gibco, NY, USA), and 100 mg/mL streptomycin (Gibco, NY, USA). ... Cell Culture and Lentivirus Transfection. Primary human umbilical vein endothelial cells (HUVECs) were purchased from the cell ... Furthermore, the overexpression of NOX2 by lentivirus abolished the protective effects of TMZ on TGF-β-induced EndMT.. ...

Plasmids and lentivirus production. View detailed protocol The doxycycline-inducible polycistronic reprogramming vector was ... 2003) Epigenetic marking correlates with developmental potential in cloned bovine preimplantation embryos Current Biology 13: ... 3. Based on the standard protocols used for the culture of fibroblasts using culture medium containing fetal bovine serum (FBS ... 3. Based on the standard protocols used for the culture of fibroblasts using culture medium containing fetal bovine serum (FBS ...

To test the lentivirus titer, MC38 or MC38-OVA cells were seeded at 5 × 105/well in 6-well plates and infected by lentivirus at ... MC38 cell line was provided by WuXi AppTec and cultured using DMEM (Gibco) with 10% fetal bovine serum (FBS), respectively. ... Lentivirus production. To produce lentivirus, 3 × 107 293FT cells were collected for reverse-transfection in each T150 flask. ... According to the virus titer, concentrated lentivirus infection at a low MOI (~0.3) was conducted for MC38 or MC38-OVA cells ...

The stable transfection of lentivirus overexpression and lentivirus-miR-sponge constructs was used to detect apoptosis in T47D- ... MCF-7 and T47D cells were cultured in DMEM-H (HyClone, USA) containing 10% fetal bovine serum (FBS) at 37 °C in a 5% CO2 ... Cultured cells were infected with 5 µg/mL polybrene and lentivirus (MOI=50) for 24 h, and then the cells were incubated with ... Stable transfection of a lentivirus-miR-sponge construct was used in T47D-CSC proliferation assays. The miR-20b-5p shRNA sponge ...

MDA-MB-231 cells were cultured in DMEM high-glucose supplemented with 10% fetal bovine serum. 1R, 5R, and control (pBabe) ... were transduced with a GFP-expressing lentivirus and GFP-expressing transductants selected by fluorescence-activated cell ... bovine serum albumin was used as serum-free starvation medium. MDA-MB-231-4173 cells (in vivo selected lung metastatic MDA-MB- ... bovine serum albumin. Primary antibody binding was detected using PE-labeled goat anti-mouse secondary antibody (Jackson ...

Avian, Bovine, Camelid, Canine, Caprine, Cervid, Equine, Exotic, Feline, Ovine, Porcine, Rodent. $0.00. Details. ... Small Ruminant Lentivirus (cELISA). Detection of antibodies to ovine progressive pneumonia (OPP) virus in ovine, and caprine ... Bovine, Camelid, Canine, Caprine, Cervid, Equine, Exotic, Feline, Ovine, Porcine, Rodent. $20.00. Details. ... Amphibian, Avian, Bovine, Canine, Caprine, Cervid, Equine, Exotic, Feline, Non Animal, Ovine, Porcine, Rodent. $16.25. Details ...

Bovine Plasma With Sodium Citrate is a high quality research product available for a wide array of chemical, biochemical and ... pLV-N-His Lentivirus Plasmid $795.00. $395.00. * Plasminogen Depleted Human Plasma $1,395.00. $695.00. ... Bovine Plasma With Sodium Citrate. $1,995.00. $995.00. Catalog Number: B2013960 (25 mL). Bovine Plasma With Sodium Citrate is a ... Bovine Collagen Type I FITC Conjugate $2,195.00. $1,095.00. * Crystallization Screen for Protein-DNA Complexes $1,295.00. $ ...

Thrombin Bovine (Highly Pure) is a high quality research product available for a wide array of chemical, biochemical and ... pLV-N-His Lentivirus Plasmid $795.00. $395.00. * Biotin Agarose Suspension (High Binding Capacity) $895.00. $495.00. ... Thrombin Bovine (Highly Pure) is a high quality Thrombin Bovine purified from Bovine Blood. This product has been used as ... Topical bovine thrombin: a 21-year review of topical bovine_thrombin spontaneous case safety reports submitted to FDAs Adverse ...

Lentivirus transduction. ETV2-encoding lentivirus and control lentivirus based on the Dox-inducible system were purchased from ... hDPSCs were isolated as previously described [20]. Cells were then cultured in a-MEM supplemented with 10% (v/v) fetal bovine ... Thereafter, hDPSCs were transduced with ETV2-encoding lentivirus or control lentivirus with or without Dox. As shown in Fig. 2c ... Based on transfection by ETV2-encoding lentivirus or control lentivirus and the presence or absence of Dox, hDPSCs were divided ...

Bovine immunodeficiency virus (BIV) is a retrovirus belonging to the genus Lentivirus. It is similar to the human ... Bovine ephemeral fever (BEF) also known as Three Day Sickness is an arthropod vector-borne disease of cattle and is caused by ... Bovine. None. Clathrin-mediated endocytosis. Budding. Cytoplasm. Cytoplasm. Zoonosis; arthropod bite: midges. ... bovine ephemeral fever virus (BEFV), a member of the genus Ephemerovirus in the family Rhabdoviridae. ...

Lentivirus production in HEK293T cells was performed as previously described [55]. MHCC97-H cells were infected with lentivirus ... fetal bovine serum. Cultures were performed at 37°C in a 5% CO2 atmosphere. ... cells infected with lentivirus were cultured for 3 weeks, stained with gentian violet, and colonies with >50 cells were counted ... Lentivirus Production and Purification. Methods Mol Biol. 2016; 1382:107-14. https://doi.org/10.1007/978-1-4939-3271-9_8 [ ...

... fetal bovine serum; shRNA: lentivirus harboring shRNA against shRNA: lentivirus harboring shRNA against mice: mice bearing an ... lentivirus harboring shRNA against control; CXCL: C-X-C motif chemokine ligand; DCs: dendritic cells; DMEM: dulbeccos modified ...

MEC-1 cells were maintained in IMDM medium, Hg3 and JVM3 in RPMI 1640 medium, both supplemented with 10% fetal bovine serum ( ... Non-integrating lentiviruses were produced using the integrase defective packaging plasmid pCMVd8.74-D64.V [15]. After ...

Using high-throughput sequencing (HTS) and bioinformatics, we discovered a new retrovirus, bovine retrovirus CH15 (BoRV CH15), ... bovine immunodeficiency virus (BIV) of the genus Lentivirus, and bovine foamy virus (BFV) of the genus Bovispumavirus [16]. BLV ... Bovine 12s rDNA qPCR. Quantitative PCR targeting the bovine 12s rDNA was performed with Power SYBR™ Green PCR Master Mix ( ... Isolation and characterization of new wild-type isolates of bovine lentivirus. J Virol. 1993;67(8):5051-5. ...

2B). We infected BMSCs with LentimiRa-GFP-rno-mir-340 lentivirus (miR-340+ BMSCs), GFP Blank miRNA lentivirus (miR-340+CON ... of fetal bovine serum (FBS; Hyclone). Cells were cultivated in LG-DMEM with 10% fetal bovine serum, 100 U/ml penicillin (Sigma ... We packaged the lentiviruses containing the vectors of LentimiRa-GFP-rno-miR-340 Vector (LentiLab, pre-miR-340 inserted for miR ... Three to four passages (P3-4) of cells were used for lentivirus infection. BMSCs were passaged or collected for injection when ...

ml) with lentivirus-mediated target gene shRNA, sgRNA, and negative control. The cells were collected after transduction for 72 ... fetal bovine serum (FBS) (Gibco, Karlsruhe, Germany) containing 1% penicillin and streptomycin solution. The cells were grown ... After transfection of sh-SCARA5 (PSC38272) lentivirus vector into the Eca109 cells for 72 h, qRT-PCR detection found that ... After transfecting Eca109 cells with shRNA lentivirus for 3 days, we spread the cells into 96-well plate, 2000 cells per well, ...

Transduction of HEPM Cells with miR-200c Using Lentivirus. In order to transduce the HEPM cells with plasmid miR-200c or ... In one embodiment, the mammal is a human, bovine, ovine, caprine, equine, porcine, canine or feline. In one embodiment, the ... For 3 days, the medium was replaced each day with fresh medium containing the same amount of lentivirus carrying miR-200c or ... In one embodiment, the mammal is a human, bovine, ovine, caprine, equine, porcine, canine or feline. In one embodiment, the ...

To prepare lentivirus particles, cells were cultured until 40-50% confluency. The medium was replaced with 10 mL of fresh DMEM ... fetal bovine serum (Gibco, CAT#10099141C) and antibacterial agent (100 U/mL penicillin, 100 µg/mL streptomycin, CAT#15140122), ... The 293T cell line (Chinese Academy of Sciences Cell Bank, CAT#GNHu17) was co-transfected with the lentivirus plasmids, ... medium (GIBCO, CAT#10566016) supplemented with 10% fetal bovine serum (Gibco, CAT#10099141C), without double antibodies. After ...

For that reason, we presume that bovine len tiviruses possess a near evolutionary connection with pri mate lentiviruses and ... Background Jembrana disease virus is usually a bovine lentivirus that in Bali cattle frequently causes an acute ailment endemic ... A common lentivirus genome is comprised of flanking long terminal repeats and three key structural genes, gag, pol, and env, ... Commonly, lentiviruses are related with persistent and progressive disorders involving a long time period of latent infection. ...

The lentivirus was collected after 48 h transfection. For the viral transduction, 786-O cells were plated in 6-well plate until ... Then, the membranes were blocked with 5% Albumin from bovine serum (BSA) and incubated in primary antibodies against targeted ... Lentiviral constructs, lentivirus production, and infection. The wild type (WT) VHL was cloned into the Tet-one vector with ... The medium was replaced with indicated lentivirus-containing medium and incubated for 24 h. 2 µg /ml puromycin (Sigma) was used ...

Lentivirus, stable cell line construction and quantitative real-time PCR. The lentiviral vector system and empty vector were ... The cell lines were cultured in Dulbeccos modified Eagle medium (DMEM) supplemented with 10% foetal bovine serum (FBS) and 1% ... 2F-I). The knockdown efficiency of UCK2 by lentivirus was detected using quantitative real-time PCR (Additional file 2: Fig. ... The knockdown efficiency of UCK2 by lentivirus was detected using quantitative real-time PCR; (D) gene expression of SULF2 in ...

E) HUVECs were transduced with lentivirus encoding TF, TFshort, or vector control and cells were analyzed for their ability to ... Controls include treatment with bovine serum albumin (BSA), a TF inhibitory antibody (anti-TF) or its isotype control, or ... HEK293T cells (ATCC) were cultured in DMEM (Life Technologies) supplemented with 10% fetal bovine serum (HyClone). Cells were ... 5 mM CaCl2 1 mg/mL bovine serum albumin) that was then replaced with HBS-BSA containing factor VIIa (0.3-0.6 nM, Haematologic ...

Lentivirus transduction and preparation. Defective-lentivirus shares had been ready as defined previously (66). In short, 1.5 ... The cells had been refed 24 h posttransfection with clean DMEM filled with 10% fetal bovine serum, and the transfection ... Lentiviruses released into the 1166393-85-6 manufacture tradition press had been harvested at 48 or 72 l posttransfection, ... For shRNA knockdown of miR-21, HEK293T or U-251MG cells had been transduced with lentiviruses (MOI = 10) extracted from pLKO.1- ...

All cells were cultured in DMEM with 10% bovine calf serum (Invitrogen) at 5% CO2. After lentivirus transfection, cells were ...