*  Mad2 and Mad3 Cooperate to Arrest Budding Yeast in Mitosis
The checkpoint is activated by kinetochores that are not attached to microtubules or are attached but not under tension and ... induces metaphase arrest that is independent of functional kinetochores or other checkpoint proteins. We further showed that ...
  https://dash.harvard.edu/handle/1/8182905
*  Budding Yeast Kinetochore Proteins, Chl4 and Ctf19, Are Required to Maintain SPB-Centromere Proximity during G1 and Late...
Budding Yeast Kinetochore Proteins, Chl4 and Ctf19, Are Required to Maintain SPB-Centromere Proximity during G1 and Late ... Previously it has been shown that the absence of non-essential kinetochore proteins of the Ctf19 complex weakens kinetochore- ... but constant kinetochore microtubule kMT dynamics, 2 poleward tension on kinetochore and the antagonistic polar ejection force ... Budding Yeast Kinetochore Proteins, Chl4 and Ctf19, Are Required to Maintain SPB-Centromere Proximity during G1 and Late ...
  http://libros.duhnnae.com/2017/jun8/149839222164-Budding-Yeast-Kinetochore-Proteins-Chl4-and-Ctf19-Are-Required-to-Maintain-SPB-Centromere-Proximity-during-G1-and-Late-Anaphase.php
*  MIS18 kinetochore protein homolog A (S. pombe) ELISA Kits | Biocompare.com
Compare MIS18 kinetochore protein homolog A (S. pombe) ELISA Kits from leading suppliers on Biocompare. View specifications, ... Your search returned 1 MIS18 kinetochore protein homolog A (S. pombe) ELISA ELISA Kit across 1 supplier. ...
  http://www.biocompare.com/pfu/110627/soids/2-39962/Assay_Kit/ELISA_MIS18_kinetochore_protein_homolog_A_S_pombe
*  Haase J et al. (2017), Distinct Roles of the Chromosomal Passenger Com... - Xenbase Paper
Although a fully assembled inner kinetochore is not required for outer kinetochore assembly, we find it is essential to recruit ... Here, we show that an activated CPC that cannot localize to centromeres supports functional assembly of the outer kinetochore ... Distinct Roles of the Chromosomal Passenger Complex in the Detection of and Response to Errors in Kinetochore-Microtubule ... We conclude centromeric CPC is necessary for tension-dependent removal of erroneous attachments and for the kinetochore ...
  http://www.xenbase.org/literature/article.do?method=display&articleId=54059
*  Equal bipolar distribution of kinetochores requires oli | Open-i
Equal bipolar distribution of kinetochores requires oligomerization of the Dam1 complexTime-lapse images of spindle poles ( ... e) Plot of kinetochore intensity ratio versus spindle length shows that asymmetric kinetochore alignment in hsk3-degron cells ... e) Plot of kinetochore intensity ratio versus spindle length shows that asymmetric kinetochore alignment in hsk3-degron cells ... 6). Asymmetric kinetochore clustering towards one pole is inconsistent with a lack of kinetochore-microtubule attachment, which ...
  https://openi.nlm.nih.gov/detailedresult.php?img=PMC4197110_nihms620354f6&req=4
*  International Review of Cytology, Vol. 136 by Kwang W. Jeon, Martin Friedlander (Eds.) - The High Fidelity Books
The authors suggest that most of their results can be explained by defects in the assembly or maturation of the kinetochores ... 1990) suggested that the CENP-B protein could be involved in either microtubule assembly at the kinetochore or in sliding of ... 1990). Injection of these purified IgGs into tissue culture cells disrupts microtubule attachment to kinetochores, chromosome ... such that either microtubules do not attach to the kinetochores or the attachment is defective. ...
  http://thehighfidelityreport.com/library/international-review-of-cytology-vol-136
*  Direct binding between BubR1 and B56-PP2A phosphatase complexes regulate mitotic progression | Journal of Cell Science
Our data suggests that BubR1 counteracts Aurora B kinase activity at improperly attached kinetochores by recruiting B56-PP2A ... In addition BubR1 also helps stabilize kinetochore-microtubule interactions by counteracting the Aurora B kinase but the ... that inhibits progression into anaphase in response to improper kinetochore-microtubule interactions. ... phosphorylation of S670 and S676 stimulates the binding of B56 to BubR1 and that BubR1 targets a pool of B56 to kinetochores. ...
  http://jcs.biologists.org/content/early/2013/01/22/jcs.122481
*  Jawaharlal Nehru Centre for Advanced Scientific Research
... a member of the evolutionarily conserved Mis12 kinetochore protein family, is required for efficient inner kinetochore assembly ... Jitendra Thakur and Kaustuv Sanyal, 'The essentiality of the fungus specific Dam1 complex is correlated with one kinetochore ...
  http://www.jncasr.ac.in/cpmupub.php?&dept_id=MBGU&yeartype=2011&empid=&pubtype=year
*  Kinetochores expand their reach | JCB
Kinetochores are thought to assemble in a hierarchical order (2). Inner kinetochore proteins, such as CENP-C and CENP-T, first ... Moreover, Wynne and Funabiki saw that the inner kinetochore protein CENP-C also localized to the expanded kinetochore filaments ... In contrast, the inner kinetochore protein CENP-T and outer kinetochore proteins involved in end-on microtubule attachment, ... "We propose that disassembly of the expanded kinetochore promotes the handover of kinetochore function from lateral microtubule ...
  http://jcb.rupress.org/content/210/6/867.full
*  Kinetochore protein Nuf2 (Q9BZD4) | InterPro | EMBL-EBI
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
  http://www.ebi.ac.uk/interpro/protein/Q9BZD4
*  Phys.org - kinetochore
Kinetochore. The kinetochore ( /kɪˈnɛtəkɔər/) is the protein structure on chromatids where the spindle fibers attach during ... The kinetochore contains two regions:. Kinetochores start, control and supervise the striking movements of chromosomes during ... which helps the kinetochore associate with DNA. Other proteins in the kinetochore attach it to the microtubules (MTs) of the ... Kinetochores prefer the 'silent' DNA sections of the chromosome. The protein complex responsible for the distribution of ...
  https://phys.org/tags/kinetochore/sort/popular/all/
*  Ran at kinetochores | Biochemical Society Transactions
... kinetochore fibre; kMT, kinetochore microtubule; LMB, leptomycin B; NPC, nuclear pore complex; RanBP2, Ran-binding protein 2; ... In addition to this role of soluble Ran-GTP, Ran has two important but less understood roles at mitotic kinetochores. Namely, ... Here, I will briefly summarize evidence for these kinetochore-associated functions and mention some of the issues that remain ... is essential for regulation of the spindle assembly checkpoint and for assembly of microtubule fibres that attach kinetochores ...
  http://www.biochemsoctrans.org/content/34/5/711
*  Analysis of kinesin motor function at budding yeast kinetochores | JCB
... kinetochore-bound Cin8p and Kip1p must cross-link MTs from different kinetochores. Metaphase sister kinetochores can ... Other yeast kinetochore proteins, including members of the MT-binding Dam1-DASH complex, require MTs for kinetochore ... How are kinesins recruited to kinetochores? In the case of Kip3p, it appears that the motor binds directly to core kinetochore ... Moreover, whereas Kar3p may function in de novo kinetochore-MT attachment, we have not been able to detect a kinetochore ...
  http://jcb.rupress.org/content/172/6/861
*  RCSB PDB - Protein Feature View - Kinetochore protein SPC25 - P40014 (SPC25 YEAST)
Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and ...
  http://www.rcsb.org/pdb/protein/P40014
*  Phosphorylation regulates targeting of cytoplasmic dynein to kinetochores during mitosis | JCB
Kinetochore labeling was evident from nuclear envelope breakdown to late prometaphase and was not detected on kinetochores ... The formation, structure, and composition of the mammalian kinetochore and kinetochore fiber. Int. Rev. Cytol. 79:1-58. ... This work provides a new model for dynein kinetics at kinetochores. Dynein is recruited to kinetochores as a phosphoprotein and ... At metaphase, when PT89-dynein labeling at kinetochores was weakest, total dynein was observed along kinetochore fibers and at ...
  http://jcb.rupress.org/content/183/5/819
*  CENP-E Function at Kinetochores Is Essential for Chromosome Alignment | JCB
Plot of kinetochore to pole distance over time for two kinetochores (Ks1P and Ks2P) of a stretched chromosome in a cell that ... The disruption of kinetochore motility when CENP-E was depleted from kinetochores led us to adopt a second approach to directly ... If the role of CENP-E is to maintain stable kinetochore microtubule connections, kinetochores that lack CENP-E may be unable to ... Microinjection of CENP-E Antibodies Depletes CENP-E from Kinetochores. Our first strategy to deplete CENP-E from kinetochores ...
  http://jcb.rupress.org/content/139/6/1373
*  Conservation of the Centromere/Kinetochore Protein ZW10 | JCB
4) are found in the vicinity of the centromere/kinetochore. This position is likely to correspond to the kinetochore or the ... Both DmZW10 and HZW10 primarily stain kinetochores in early anaphase (Fig. 6, E and F), but HZW10 is lost from the kinetochores ... Conservation of the Centromere/Kinetochore Protein ZW10. Daniel A. Starr, Byron C. Williams, Zexiao Li, Bijan Etemad-Moghadam, ... appearing to move from the centromere/kinetochore at prometaphase to kinetochore microtubules at metaphase, and back to the ...
  http://jcb.rupress.org/content/138/6/1289?ijkey=b8f73ff3f4cee63f88188497fbfbf3854da8faec&keytype2=tf_ipsecsha
*  Polyploids require Bik1 for kinetochore-microtubule attachment | JCB
A crucial step in mitosis is the attachment of spindle microtubules (MTs)* to the kinetochores: sister kinetochores must form ... bik1-CTΔ40-containing triploids have a defect in preanaphase kinetochore separation. Kinetochore separation was scored in the ... MT-independent binding of Bik1 to the kinetochore. Because of the prominent Bik1 signal at kinetochores, chromatin ... Unseparated kinetochores are seen as one fluorescent dot, whereas separated kinetochores are seen as two dots. The fraction of ...
  http://jcb.rupress.org/content/155/7/1173
*  The Kinetochore Receptor for the Cohesin Loading Complex
... Author: Hinshaw, Stephen M.; Makrantoni, Vasso; Harrison, Stephen C. ... The Kinetochore Receptor for the Cohesin Loading Complex. DSpace/Manakin Repository. * DASH Home ... The kinetochore, a multiprotein complex that connects centromeres to microtubules, drives the recruitment of high levels of ... We show that phosphorylation of the Ctf19 kinetochore protein by a conserved kinase, DDK, provides a binding site for the Scc2/ ...
  https://dash.harvard.edu/handle/1/34492077
*  Differential expression of a phosphoepitope at the kinetochores of moving chromosomes | JCB
... one kinetochore is intensely labeled while its sister kinetochore is unlabeled. Chromosomes moving toward the metaphase plate ... In mid-prometaphase, some chromosomes show strong labeling on both kinetochores; others exhibit weak or no labeling; while in ... Differential expression of a phosphoepitope at the kinetochores of moving chromosomes. GJ Gorbsky, WA Ricketts ... A phosphorylated epitope is differentially expressed at the kinetochores of chromosomes in mitotic cells and may be involved in ...
  http://jcb.rupress.org/content/122/6/1311
*  Mislocalization of the Drosophila centromere-specific histone CIDpromotes formation of functional ectopic kinetochores -...
In addition, proteins that are normally restricted to endogenous kinetochores assemble at a subset of ectopic CID incorporation ... and aberrant chromosome morphologies demonstrate that these ectopic kinetochores are functional. We conclude that CID ... is a structural and functional foundation for kinetochore formation and chromosome segregation. Here, we show that ... The centromere-specific histone variant CENP-A (CID in Drosophila) is a structural and functional foundation for kinetochore ...
  https://digital.library.unt.edu/ark:/67531/metadc882613/
*  Nuf2 MGI Mouse Gene Detail - MGI:1914227 - NUF2, NDC80 kinetochore complex component
View mouse Nuf2 Chr1:169497934-169531464 with: sequences, polymorphisms, proteins, references, function, expression
  http://www.informatics.jax.org/marker/MGI:1914227
*  MCAK facilitates chromosome movement by promoting kinetochore microtubule turnover | JCB
MCAK increases kinetochore MT turnover. The effects of changing MCAK levels on kinetochore attachment were too subtle to ... We used the appearance of detyronsinated tubulin (glu-tubulin) in kinetochore fibers as a read-out for kinetochore fiber ... We did not administer ZM447439 to specifically influence MCAK but to lock kinetochores more tightly onto kinetochore fibers ( ... Our observations have two implications for kinetochore function: one is that dynamic instability within the kinetochore fiber ...
  http://jcb.rupress.org/content/179/5/869
*  Importin-β negatively regulates multiple aspects of mitosis including RANGAP1 recruitment to kinetochores | JCB
Kinetochore-driven formation of kinetochore fibers contributes to spindle assembly during animal mitosis. J. Cell Biol. 167:831 ... kinetochore. MT. microtubule. NE. nuclear envelope. NLS. nuclear localization signal. NPC. nuclear pore complex. NUP. ... Ran-GTP regulates kinetochore attachment in somatic cells. Cell Cycle. 4:1161-1165. doi:10.4161/cc.4.9.1979. ... Molecular requirements for kinetochore-associated microtubule formation in mammalian cells. Curr. Biol. 16:536-541. doi:10.1016 ...
  http://jcb.rupress.org/content/196/4/435