Kinetochore - Wikipedia
The kinetochore contains two regions: an inner kinetochore, which is tightly associated with the centromere DNA and assembled ... The movement towards the pole is slowed down as far as kinetochores acquire kMTs (MTs anchored to kinetochores) and the ... These experiments also showed that kinetochores have polarity, and that kinetochore attachment to MTs emanating from one or the ... The actual set of genes essential for kinetochore function varies from one species to another. Kinetochore functions include ...
Paired arrangement of kinetochores together with microtubule pivoting and dynamics drive kinetochore capture in meiosis I |...
We show that kinetochores on homologous chromosomes move together, microtubules are dynamic and pivot around the spindle pole, ... In the present study, we combine experiments and theory to explore the mechanisms of kinetochore capture at the onset of ... Kinetochore pairing may be a general feature that increases capture efficiency in meiotic cells. ... Our theory describes paired kinetochores on homologous chromosomes as a single object, as well as angular movement of ...
Springs, clutches and motors: driving forward kinetochore mechanism by modelling
... the problem of kinetochore mechanism is now one of understanding how these functional modules assemble, disassemble and ... the kinetochore can be viewed as a set of interacting springs, clutches and motors; ... Springs, clutches and motors: driving forward kinetochore mechanism by modelling Chromosome Res. 2011 Apr;19(3):409-21. doi: ... As a mechanical system, the kinetochore can be viewed as a set of interacting springs, clutches and motors; the problem of ...
Astrin-SKAP complex reconstitution reveals its kinetochore interaction with microtubule-bound Ndc80
... Author(s). Su, Kuan-Chung ... A key outstanding question is how kinetochore-microtubule attachments are modulated to ensure that bi-oriented attachments are ... The Astrin-SKAP complex localizes preferentially to properly bi-oriented sister kinetochores, representing the final outer ... Our work demonstrates that the Astrin-SKAP complex contains separable kinetochore localization and microtubule binding domains ...
Kinetochores associated with the nuclear envelope in the mitosis of a dinoflagellate
Iain Cheeseman: A strong attachment to kinetochores | Journal of Cell Biology | Rockefeller University Press
Lots of human kinetochore proteins dont exist in C. elegans and I was curious about how they work. And it would be technically ... But kinetochores are much more than a simple link between DNA and microtubules-they coordinate chromosome and spindle dynamics ... I decided to do a postdoc in a kinetochore laboratory, partly because I came to it a little bit later in my PhD. ... Even going back 100 years, there was a good understanding of what a kinetochore is and for the next, say, 60 years people were ...
Chromatin Assembly at Kinetochores Is Uncoupled from DNA Replication | Journal of Cell Biology | Rockefeller University Press
1997). Thus, if CENP-A expression is coupled to kinetochore DNA replication, then kinetochore DNA replication must occur quite ... Chromatin Assembly at Kinetochores Is Uncoupled from DNA Replication Richard D. Shelby, Richard D. Shelby ... B). Kinetochores were first labeled by expression of CENP-A-HA1 and then repressed as described for Fig. 1. Since CENP-A-HA1 is ... Kinetochore labeling was performed by inducing CENP-A-HA1 expression in HeLa Tta-CENP-A-HA1 cells for 2 d (Shelby et al. 1997 ...
Augmin accumulation on long-lived microtubules drives amplification and kinetochore-directed growth. | Janelia Research Campus
Augmin accumulation on long-lived microtubules drives amplification and kinetochore-directed growth. Betzig Lab ... Our systematic quantification of spindle dynamics reveals highly coordinated microtubule growth during kinetochore fiber ... Augmin accumulation on long-lived microtubules drives amplification and kinetochore-directed growth.. ... we observed that this mechanism results in a strong directional bias of microtubule growth toward individual kinetochores. ...
Kinetochore-microtubule interaction during S phase in Saccharomyces cerevisiae<...
Soon afterward, kinetochores are reassembled, leading to their recapture by microtubules. We also show how kinetochores are ... Soon afterward, kinetochores are reassembled, leading to their recapture by microtubules. We also show how kinetochores are ... Soon afterward, kinetochores are reassembled, leading to their recapture by microtubules. We also show how kinetochores are ... Soon afterward, kinetochores are reassembled, leading to their recapture by microtubules. We also show how kinetochores are ...
kinetochores
Tag Archives: kinetochores. Journal Club on Monday, August 6 For Journal Club on August 6, Nate Krefman presented the following ... This entry was posted in Announcements and tagged announcement, biorientation, cell biology, cell cycle control, kinetochore- ... kinetochores, mechanism, mitosis, molecular biology, plasmids on September 2, 2013. by NateKrefman. Journal Club on Monday, ... kinetochores, mechanism, meiosis, microtubules, Science, yeast on September 2, 2013. by NateKrefman. Microtubules and Mitosis ...
GO:0000776: kinetochore details
Synthetic physical interactions map kinetochore-checkpoint activation regions
Mad1Mad2Mps1SACkinetochoreCell Cycle CheckpointsCell Cycle ProteinsChromosomal Proteins, Non-HistoneDNA-Binding Proteins ... The recruitment of the Mad1 and Mad2 proteins to the kinetochore is normally necessary for SAC activation. This recruitment is ... and Mps1 to most proteins in the yeast kinetochore, and find that, while Mps1 is sufficient for checkpoint activation, ... KinetochoresMad2 ProteinsMicroscopy, FluorescenceMutationNuclear ProteinsPhosphorylationProtein-Serine-Threonine Kinases ...
System that controls cell division resembles a mosaic - News - Utrecht University
The kinetochore in every eukaryotic cell on Earth has a complex structure, while prokaryotic cells dont have a kinetochore. In ... Mosaic origin of the eukaryotic kinetochore. Eelco Tromer*^, Jolien J.E. van Hooff*^, Geert J.P.L. Kops^ and Berend Snel*. PNAS ... Kinetochore gives starting shot for metaphase Geert Kops is Professor of Molecular Tumour Cell Biology at the Hubrecht ... To me, the kinetochore is the black hole of eukaryotic cell division. We would very much like to know how the system developed ...
Crystal structure of the yeast inner kinetochore subunit Cep3p. | NECAT
Home » Crystal structure of the yeast inner kinetochore subunit Cep3p.. Crystal structure of the yeast inner kinetochore ... Amino Acid Sequence, Base Sequence, Centromere, Crystallography, X-Ray, DNA, Fungal, DNA-Binding Proteins, Kinetochores, Models ... p,In budding yeast, the four-protein CBF3 complex (Skp1p-Ctf13p-Cep3p-Ndc10p) initiates kinetochore assembly by binding to the ... Cep3p scaffolds kinetochore assembly on centromeres uniformly configured on all 16 yeast chromosomes. We have, using the ...
Divergent polo box domains underpin the unique kinetoplastid kinetochore. - Oxford Neuroscience
... is not a structural kinetochore component in either system. Here, we report the identification of an additional kinetochore ... These divergent PBDs of KKT2 and KKT20 are sufficient for kinetochore localization in vivo. We propose that the ancestral PLK ... While most eukaryotes possess conventional kinetochore proteins, evolutionarily distant kinetoplastid species have ... acquired a Cys-rich region and then underwent gene duplication events to give rise to three structural kinetochore proteins in ...
MiCroKiTS - An Integrated Database of Midbody, Centrosome, Kinetochore, Telomere and Spindle
Planteome: Term Details for 'positive regulation of kinetochore assembly' (GO:1905561)
positive regulation of kinetochore assembly RO:0002213 kinetochore assembly (GO:0051382) positive regulation of kinetochore ... up regulation of kinetochore assembly, up-regulation of kinetochore assembly, upregulation of kinetochore assembly, activation ... positive regulation of kinetochore formation, up regulation of kinetochore formation, up-regulation of kinetochore formation, ... positive regulation of kinetochore assembly is_a regulation of kinetochore assembly (GO:0090234) ...
Removal of Spindly from microtubuleattached kinetochores controls spindle checkpoint silencing in human cells • MatTek Life...
Cyclin B1 scaffolds MAD1 at the kinetochore | Max Planck Institute of Molecular Physiology
Structure of the MIND Complex Defines a Regulatory Focus for Yeast Kinetochore Assembly. | NECAT
Structure of the MIND Complex Defines a Regulatory Focus for Yeast Kinetochore Assembly.. Publication Type:. Journal Article ... p,Kinetochores connect centromeric nucleosomes with mitotic-spindle microtubules through conserved, cross-interacting protein ... Chromosomal Proteins, Non-Histone, Crystallography, X-Ray, Fungal Proteins, Kinetochores, Kluyveromyces, Multiprotein Complexes ... Dsn1 phosphorylation by Ipl1/Aurora B relieves this autoinhibition, enabling MIND to join an assembling kinetochore. A C- ...
Cdc14-dependent dephosphorylation of a kinetochore protein prior to anaphase in Saccharomyces cerevisiae. - Immunology
Although Cdc14 activity is thought to be restricted to anaphase, we found that dephosphorylation of the Dsn1 kinetochore ... Cdc14-dependent dephosphorylation of a kinetochore protein prior to anaphase in Saccharomyces cerevisiae. ... Although Cdc14 activity is thought to be restricted to anaphase, we found that dephosphorylation of the Dsn1 kinetochore ... Anaphase, CDC2 Protein Kinase, Cell Cycle Proteins, Chromosomal Proteins, Non-Histone, Kinetochores, Mitosis, Phosphorylation, ...
The kinesin-8 Kif18A dampens microtubule plus-end dynamics
At kinetochore-microtubule plus ends, the kinesin-8 family member Kif18A is essential to align mitotic chromosomes at the ... At kinetochore-microtubule plus ends, the kinesin-8 family member Kif18A is essential to align mitotic chromosomes at the ... have developed biochemically distinct activities throughout evolution and have implications for how Kif18A affects kinetochore- ...
Carlos PENDON | Head of Department | Dr | Universidad de Cádiz, Cadiz | UCA | Department of Biomedicine, Biotechnology and...
Regulation of kinetochore and inner-centromere structure and function by the Chromosome Passenger Complex during mitosis
activity at the kinetochore changes in response to the kinetochore-microtubule attachment status and this change is important ... outer kinetochore maintenance is dependent on the CPC and Plk1 activity before but not after mature kinetochore-microtubule ... Apart from regulating kinetochore-microtubule attachment Aurora-B also regulates the assembly of the outer kinetochore during ... outer kinetochore organization is thought to remain unchanged before and after kinetochore-microtubule attachment. It is thus ...
Hutch News | Fred Hutchinson Cancer Center
Discovering the impact of nontolerant variants in kinetochore genes using cutting-edge super-resolution live-cell microscopy
... ... To predict those that are harmful, the student will use published structures of kinetochore proteins. To investigate the impact ... The extent to which naturally occurring variations in kinetochore genes can promote chromosomal instability is not clear, ... Chromosome-microtubule attachment is facilitated by a macromolecular structure called the kinetochore. ...
The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation<...
Hsu, JM, Huang, J, Meluh, PB & Laurent, BC 2003, The yeast RSC chromatin-remodeling complex is required for kinetochore ... The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation. In: Molecular and ... This study reveals an important role for the yeast RSC ATP-dependent chromatin-remodeling complex at the kinetochore in ... The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation. / Hsu, Jing Mei; ...
The chromosomal passenger complex and the spindle assembly checkpoint: kinetochore-microtubule error correction and beyond ::...
컨텐츠 | Nature Portfolio
Kinetochore geometry defined by cohesion within the centromere p.852 doi: 10.1038/nature07876 ...