*  Cell division and reproduction - It's a natural universe
During meiosis II, a kinetochore is formed on each sister chromatid. Phases similar to those of meiosis I take place until, in ... attach via kinetochores to the centromeres of the chromosomes, i.e., to sister pairs. These connections are also random. Any ... with sister chromatids pulled towards opposite poles of the cell by the kinetochores, which crawl along the spindle tubules ... since the kinetochores are attached to the centromeres. ... of the spindle by protein complexes called kinetochores .. * ...
  http://natural-universe.net/the-scientific-view-of-the-universe/the-geological-present/what-biochemistry-and-cellular-biology-tell-us/cell-division-the-cell-cycle/?print=print
*  BRK1, a Bub1-Related Kinase, Is Essential for Generating Proper Tension between Homologous Kinetochores at Metaphase I of Rice...
To find out what caused the reduced tension across homologous kinetochores, we detected the kinetochore-microtubule attachment ... Supplemental Figure 5. The Dissociation of BRK1 from Kinetochore Is Independent of Kinetochore-Microtubule Attachment. ... both sister kinetochores are attached to the same spindle pole), and merotelic (one kinetochore is attached to two opposing ... and its kinetochore levels are less affected by the kinetochore-microtubule attachment. The only stages during which BRK1 ...
  http://www.plantcell.org/content/24/12/4961.full
*  Ran at kinetochores | Biochemical Society Transactions
... kinetochore fibre; kMT, kinetochore microtubule; LMB, leptomycin B; NPC, nuclear pore complex; RanBP2, Ran-binding protein 2; ... In addition to this role of soluble Ran-GTP, Ran has two important but less understood roles at mitotic kinetochores. Namely, ... Here, I will briefly summarize evidence for these kinetochore-associated functions and mention some of the issues that remain ... is essential for regulation of the spindle assembly checkpoint and for assembly of microtubule fibres that attach kinetochores ...
  http://www.biochemsoctrans.org/content/34/5/711
*  Kinetochores accelerate centrosome separation to ensure faithful chromosome segregation | Journal of Cell Science
Kinetochores accelerate centrosome separation to ensure faithful chromosome segregation Message Subject (Your Name) has sent ... Kinetochores accelerate centrosome separation to ensure faithful chromosome segregation. Nunu Mchedlishvili, Samuel Wieser, ... Kinetochores accelerate centrosome separation to ensure faithful chromosome segregation. Nunu Mchedlishvili, Samuel Wieser, ... Kinetochores accelerate centrosome separation to ensure faithful chromosome segregation. Nunu Mchedlishvili, Samuel Wieser, ...
  http://jcs.biologists.org/content/early/2012/03/07/jcs.091967
*  Release of fluorescent Mad2 particles from kinetochores | Open-i
Fluorescent XMad2 particles were released from the kinetochore and transported towards the spindle pole ... Finally, in normal cells, the half-life of Mad2 turnover at poles, 23 s, was similar to kinetochores. Thus, kinetochore-derived ... Finally, in normal cells, the half-life of Mad2 turnover at poles, 23 s, was similar to kinetochores. Thus, kinetochore-derived ... Recent studies have suggested a catalytic model for kinetochore function where unattached kinetochores provide sites for ...
  https://openi.nlm.nih.gov/detailedresult.php?img=PMC2150717_JCB0003130.f8&req=4
*  Mad2 and Mad3 Cooperate to Arrest Budding Yeast in Mitosis
The checkpoint is activated by kinetochores that are not attached to microtubules or are attached but not under tension and ... induces metaphase arrest that is independent of functional kinetochores or other checkpoint proteins. We further showed that ...
  https://dash.harvard.edu/handle/1/8182905
*  Budding Yeast Kinetochore Proteins, Chl4 and Ctf19, Are Required to Maintain SPB-Centromere Proximity during G1 and Late...
Budding Yeast Kinetochore Proteins, Chl4 and Ctf19, Are Required to Maintain SPB-Centromere Proximity during G1 and Late ... Previously it has been shown that the absence of non-essential kinetochore proteins of the Ctf19 complex weakens kinetochore- ... but constant kinetochore microtubule kMT dynamics, 2 poleward tension on kinetochore and the antagonistic polar ejection force ... Budding Yeast Kinetochore Proteins, Chl4 and Ctf19, Are Required to Maintain SPB-Centromere Proximity during G1 and Late ...
  http://libros.duhnnae.com/2017/jun8/149839222164-Budding-Yeast-Kinetochore-Proteins-Chl4-and-Ctf19-Are-Required-to-Maintain-SPB-Centromere-Proximity-during-G1-and-Late-Anaphase.php
*  Switching a Checkpoint On and Off Again | Science Signaling
... of the chromosomes are attached to spindle microtubules and until tension is being applied to pull the sister kinetochores ... that ensures accurate distribution of the chromosomes during cell division by delaying completion of mitosis until kinetochores ...
  http://stke.sciencemag.org/content/2006/347/tw270
*  Regulation of kinetochore-microtubule attachments by Aurora B kinase | Biochemical Society Transactions
Regulation of kinetochore-microtubule attachments by Aurora B kinase Message Subject (Your Name) has forwarded a page to you ... Regulation of kinetochore-microtubule attachments by Aurora B kinase. Dan Liu, Michael A. Lampson ... Accurate segregation of chromosomes in mitosis requires that spindle microtubules attach sister kinetochores to opposite poles ... Destabilization of incorrect attachments requires the Ipl1/Aurora B kinase, which phosphorylates kinetochore substrates that ...
  http://www.biochemsoctrans.org/content/37/5/976
*  Centromeres And Kinetochores: Discovering The Molecular Mechanisms Underlying Chromosome Inheritance (progress In Molecular And...
Read Online or Download Centromeres And Kinetochores: Discovering The Molecular Mechanisms Underlying Chromosome Inheritance ( ... Centromeres And Kinetochores: Discovering The Molecular Mechanisms Underlying Chromosome Inheritance (progress In Molecular And ...
  https://mytxt.xyz/centromeres-and-kinetochores-discovering-the-molecular-mechanisms-underlying-chromosome-inheritance-progress-in-molecular-and-subcellular-biology/
*  Haase J et al. (2017), Distinct Roles of the Chromosomal Passenger Com... - Xenbase Paper
Although a fully assembled inner kinetochore is not required for outer kinetochore assembly, we find it is essential to recruit ... Here, we show that an activated CPC that cannot localize to centromeres supports functional assembly of the outer kinetochore ... Distinct Roles of the Chromosomal Passenger Complex in the Detection of and Response to Errors in Kinetochore-Microtubule ... We conclude centromeric CPC is necessary for tension-dependent removal of erroneous attachments and for the kinetochore ...
  http://www.xenbase.org/literature/article.do?method=display&articleId=54059
*  Equal bipolar distribution of kinetochores requires oli | Open-i
Equal bipolar distribution of kinetochores requires oligomerization of the Dam1 complexTime-lapse images of spindle poles ( ... e) Plot of kinetochore intensity ratio versus spindle length shows that asymmetric kinetochore alignment in hsk3-degron cells ... e) Plot of kinetochore intensity ratio versus spindle length shows that asymmetric kinetochore alignment in hsk3-degron cells ... 6). Asymmetric kinetochore clustering towards one pole is inconsistent with a lack of kinetochore-microtubule attachment, which ...
  https://openi.nlm.nih.gov/detailedresult.php?img=PMC4197110_nihms620354f6&req=4
*  Roles of the Drosophila NudE protein in kinetochore function and centrosome migration | Journal of Cell Science
... whereas yeast kinetochores do not. At least in some metazoan cell types, dynein at the kinetochores helps move the chromosomes ... which was subsequently shown to be a human kinetochore component needed for kinetochore targeting of RZZ (Starr et al., 2000; ... NudE is found either at the kinetochores or simultaneously at the kinetochores and along the spindle (Fig. 1B; supplementary ... Targeting of other kinetochore proteins in nudE mutants. We examined the effects of homozygosity for the nudE39A null mutation ...
  http://jcs.biologists.org/content/122/11/1747
*  International Review of Cytology, Vol. 136 by Kwang W. Jeon, Martin Friedlander (Eds.) - The High Fidelity Books
The authors suggest that most of their results can be explained by defects in the assembly or maturation of the kinetochores ... 1990) suggested that the CENP-B protein could be involved in either microtubule assembly at the kinetochore or in sliding of ... 1990). Injection of these purified IgGs into tissue culture cells disrupts microtubule attachment to kinetochores, chromosome ... such that either microtubules do not attach to the kinetochores or the attachment is defective. ...
  http://thehighfidelityreport.com/library/international-review-of-cytology-vol-136
*  Merotelic kinetochore orientation occurs frequently during early mitosis in mammalian tissue cells and error correction is...
The prometaphase-I kinetochore microtubule bundle and kinetochore orientation in males. J. Cell Biol. 93, 365-373. ... Merotelic kinetochore orientation is an error that occurs when a single kinetochore becomes attached to microtubules from two ... Merotelic kinetochore orientation is an error in kinetochore function and represents a major source of aneuploidy in mammalian ... Kinetochore microtubules are infrequently assembled from kinetochores and their minus-ends recruited to the spindle poles ( ...
  http://jcs.biologists.org/content/116/20/4213
*  Direct binding between BubR1 and B56-PP2A phosphatase complexes regulate mitotic progression | Journal of Cell Science
Our data suggests that BubR1 counteracts Aurora B kinase activity at improperly attached kinetochores by recruiting B56-PP2A ... In addition BubR1 also helps stabilize kinetochore-microtubule interactions by counteracting the Aurora B kinase but the ... that inhibits progression into anaphase in response to improper kinetochore-microtubule interactions. ... phosphorylation of S670 and S676 stimulates the binding of B56 to BubR1 and that BubR1 targets a pool of B56 to kinetochores. ...
  http://jcs.biologists.org/content/early/2013/01/22/jcs.122481
*  Title page for ETD etd-06102011-075914
Changes in Kinetochore Structure and Molecular Composition in Response to Mis-attachment. ... in which the two sister kinetochores (KTs) are attached to microtubules (MTs) from opposite mitotic spindle poles. KT mis- ...
  http://theses.lib.vt.edu/theses/available/etd-06102011-075914/
*  Kinetochores expand their reach | JCB
Kinetochores are thought to assemble in a hierarchical order (2). Inner kinetochore proteins, such as CENP-C and CENP-T, first ... Moreover, Wynne and Funabiki saw that the inner kinetochore protein CENP-C also localized to the expanded kinetochore filaments ... In contrast, the inner kinetochore protein CENP-T and outer kinetochore proteins involved in end-on microtubule attachment, ... "We propose that disassembly of the expanded kinetochore promotes the handover of kinetochore function from lateral microtubule ...
  http://jcb.rupress.org/content/210/6/867.full
*  Kinetochore protein Nuf2 (Q9BZD4) | InterPro | EMBL-EBI
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
  http://www.ebi.ac.uk/interpro/protein/Q9BZD4
*  Phys.org - kinetochore
Kinetochore. The kinetochore ( /kɪˈnɛtəkɔər/) is the protein structure on chromatids where the spindle fibers attach during ... The kinetochore contains two regions:. Kinetochores start, control and supervise the striking movements of chromosomes during ... which helps the kinetochore associate with DNA. Other proteins in the kinetochore attach it to the microtubules (MTs) of the ... Kinetochores prefer the 'silent' DNA sections of the chromosome. The protein complex responsible for the distribution of ...
  https://phys.org/tags/kinetochore/sort/popular/all/
*  Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore. - PubMed - NCBI
... the inner kinetochore-centromere relaxed faster than the outer kinetochore. Whereas in fission yeast all kinetochores relaxed ... Our study establishes merotelic kinetochores as an experimental model for studying the mechanical response of the kinetochore ... Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore.. Cojoc G1, Roscioli E2, Zhang L3, García-Ulloa A ... Upon microtubule interaction, kinetochores are subjected to forces generated by the microtubules. In this work, we used laser ...
  https://www.ncbi.nlm.nih.gov/pubmed/27002163?dopt=Abstract
*  Analysis of kinesin motor function at budding yeast kinetochores | JCB
... kinetochore-bound Cin8p and Kip1p must cross-link MTs from different kinetochores. Metaphase sister kinetochores can ... Other yeast kinetochore proteins, including members of the MT-binding Dam1-DASH complex, require MTs for kinetochore ... How are kinesins recruited to kinetochores? In the case of Kip3p, it appears that the motor binds directly to core kinetochore ... Moreover, whereas Kar3p may function in de novo kinetochore-MT attachment, we have not been able to detect a kinetochore ...
  http://jcb.rupress.org/content/172/6/861
*  RCSB PDB - Protein Feature View - Kinetochore protein SPC25 - P40014 (SPC25 YEAST)
Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and ...
  http://www.rcsb.org/pdb/protein/P40014
*  Phosphorylation regulates targeting of cytoplasmic dynein to kinetochores during mitosis | JCB
Kinetochore labeling was evident from nuclear envelope breakdown to late prometaphase and was not detected on kinetochores ... The formation, structure, and composition of the mammalian kinetochore and kinetochore fiber. Int. Rev. Cytol. 79:1-58. ... This work provides a new model for dynein kinetics at kinetochores. Dynein is recruited to kinetochores as a phosphoprotein and ... At metaphase, when PT89-dynein labeling at kinetochores was weakest, total dynein was observed along kinetochore fibers and at ...
  http://jcb.rupress.org/content/183/5/819