What does ionomycin mean?
Meaning of ionomycin. What does ionomycin mean? Information and translations of ionomycin in the most comprehensive dictionary ... Definition of ionomycin in the Definitions.net dictionary. ... What does ionomycin mean?. Definitions for ionomycin. Here are ... Ionomycin. Ionomycin is an ionophore produced by the bacterium Streptomyces conglobatus. It is used in research to raise the ... Discuss these ionomycin definitions with the community:. Word of the Day. Would you like us to send you a FREE new word ...http://www.definitions.net/definition/ionomycin
Immunology] mechanisms of cytokine induction
I have noticed that ConA, PHA and PMA/ionomycin differently induce similar cytokines in T cells. Please could anyone explain to ...http://www.bio.net/bionet/mm/immuno/2006-August/017876.html
Ionomycin - Wikipedia
Commercially, Ionomycin can be obtained as a free acid, or as a Ca2+ salt. See Ionomycin Calcium salt Archived 2006-06-26 at ... Ionomycin is an ionophore produced by the bacterium Streptomyces conglobatus. It is used in research to raise the intracellular ... from Fermentek, manufacturer's product page for ionomycin. ...https://en.wikipedia.org/wiki/Ionomycin
AID 209814 - In vitro inhibition of proliferation of PMA and ionomycin stimulated murine T cells; Antagonist - PubChem
In vitro inhibition of proliferation of PMA and ionomycin stimulated murine T cells; Antagonist. ...https://pubchem.ncbi.nlm.nih.gov/bioassay/209814
AID 1270 - PMA/Ionomycin-induced IL-8 production in HEK293- NF-kB-Luc stable cells. - PubChem
PMA/Ionomycin-induced IL-8 production in HEK293- NF-kB-Luc stable cells.. ...https://pubchem.ncbi.nlm.nih.gov/bioassay/1270
AID 1266 - PMA/Ionomycin induced NF-kB luciferase assay in 293- NF-kB-Luc stable cells - PubChem
PMA/Ionomycin induced NF-kB luciferase assay in 293- NF-kB-Luc stable cells. ...https://pubchem.ncbi.nlm.nih.gov/bioassay/1266
... , a new polyether antibiotic. Liu W-C. et al. J. Antibiot. 1978, 31, 815.. Characterization of ionomycin as a calcium ... More recently, ionomycin has been used in cell biology as a universal calcium ionophore to explore the role of calcium ... Ionomycin is soluble in ethanol, methanol, DMF or DMSO. Poor water solubility. CAS Number. 56092-81-0 ... Ionomycin was isolated from Streptomyces conglobatus as a potent Gram positive antibiotic. During isolation, it was recognised ...https://www.toku-e.com/Ionomycin-P3165.aspx
Ionomycin, Free Acid, Streptomyces conglobatus in Solution - CAS 56092-81-0 - Calbiochem CAS 56092-81-0 | 407951
Ionomycin, Free Acid, Streptomyces conglobatus in Solution - CAS 56092-81-0 - Calbiochem Find MSDS or SDS, a COA, data sheets ... A 5 mM (1 mg/282 µl) solution of Ionomycin, Free Acid, Streptomyces conglobatus (Cat. No. 407950) in DMSO.. ... A 5 mM (1 mg/282 µl) solution of Ionomycin, Free Acid, Streptomyces conglobatus (Cat. No. 407950) in DMSO.. ... 407951 , Ionomycin, Free Acid, Streptomyces conglobatus in Solution - CAS 56092-81-0 - Calbiochem. ...http://www.emdmillipore.com/US/en/product/Ionomycin-Free-Acid-Streptomyces-conglobatus-in-Solution-CAS-56092-81-0-Calbiochem,EMD_BIO-407951
Ionomycin for 6.2 | ISB Server Wahoo
3 µM Ionomycin in Ca-free HBS. Dilute stock 1:333 in Ca-free HBS: 3 µL/1 mL. 45 µL in 15 mL ... 3 µM Ionomycin in HBS. Dilute stock 1:333 in HBS: 3 µL/1 mL. ... Ionomycin stock. Fisher or Invitrogen (Life Technologies) ...http://wahoo.cns.umass.edu/content/ionomycin-62
Signal Transduction Reagents and Kits
Alfa Aesar™ Ionomycin Calcium Salt, 99%. Highly selective and potent calcium ionophore ...https://www.fishersci.com/us/en/products/I9C8KX5Y/signal-transduction-reagents-kits.html
Fluo-3, Pentaammonium Salt | Biotium
Ionomycin, calcium salt Ionomycin is commonly used to modify intracellular calcium concentrations and to calibrate fluorescent ...https://biotium.com/product/fluo-3-pentaammonium-salt/
The Physical Association of Protein Kinase Cθ with a Lipid Raft-Associated Inhibitor of κB Factor Kinase (IKK) Complex Plays a...
Cellular stimulation with mAbs and PMA (P) plus ionomycin (I). The Jurkat T cell clone BMS2 and resting primary human CD4+ T ... ionomycin; Luc, luciferase; DIM, detergent-insoluble material; DSM, detergent-soluble material; wt, wild type; DN, dominant ...http://www.jimmunol.org/content/165/12/6933?ijkey=384d2661e2536839b86ef54ad58e9f883cdda92b&keytype2=tf_ipsecsha
Biosynthesis of an Endogenous Cannabinoid Precursor in Neurons and its Control by Calcium and cAMP | Journal of Neuroscience
a, Ionomycin (1 μm, 10 min) stimulates [3H]NAPE biosynthesis in neurons labeled by incubation with [3H]ethanolamine (Eth), [3H] ... Potentiation of ionomycin (iono)-stimulated [3H]NAPE biosynthesis by forskolin (FSK; 10 μm) and vasoactive intestinal peptide ( ... c, d, Ionomycin stimulates NAPE biosynthesis in neurons (c), but not in astrocytes (d). Cultures were labeled by overnight ... Representative chromatograms from (a) control neurons, (b) 1 μm ionomycin-stimulated neurons, and (c) control neurons to which ...http://www.jneurosci.org/content/16/12/3934/tab-figures-data
Calcium Ionophore Solution Can Increase Fertilization Rate in Patients During Intracytoplasmic Sperm Injection(ICSI) Who Have...
Ionomycin. Calcium Ionophores. Bone Density Conservation Agents. Physiological Effects of Drugs. Membrane Transport Modulators ... oocytes are incubated for 15 minutes in a 30 microlitre droplet of calcium ionomycin solution ...https://clinicaltrials.gov/ct2/show/NCT02045914?recr=Open&intr=dietary+calcium&rank=13
Integration of Kinase and Calcium Signaling at the Level of Chromatin Underlies Inducible Gene Activation in T Cells | The...
Cells stimulated with PMA (20 ng/ml), ionomycin (1 μg/ml), or PMA/ionomycin (20 ng/ml PMA/1 μg/ml ionomycin) for 15 and 60 min ... 1 μg/ml ionomycin, or PMA and ionomycin (20 ng/ml PMA/1 μg/ml ionomycin) for 3 h, and DNase-seq profiles of human T ... secreted by Jurkat T cells treated with PMA/ionomycin (20 ng/ml PMA/1 μg/ml ionomycin), ionomycin (1 μg/ml), or PMA (20 ng/ml ... B-D) Profiles of the ATAC-seq signals within 2-kb windows centered on each peak for PMA/ionomycin (B), PMA (C), and ionomycin ( ...http://www.jimmunol.org/content/199/8/2652
Store-Operated Calcium Entry through Orai Is Required for Transcriptional Maturation of the Flight Circuit in Drosophila |...
Ionomycin (10 μm; Calbiochem-407950, Merck Millipore) was added after 40 frames or 600 s so as to confirm that Fluo-4 levels ... Addition of thapsigargin, CaCl2, and ionomycin was done manually at the indicated time points. Images were acquired ... Ionomycin. F, The range of maximum peak intensity is lowest in orai3 compared with the indicated genotypes shown as box plots. ...http://www.jneurosci.org/content/35/40/13784?ijkey=cb988e4124a69f65983e362f1758c948cc0736de&keytype2=tf_ipsecsha
... for CTL and ionomycin-treated cells, mean ± SD; n = 3) (Fig. 2A,B). As expected, ionomycin treatment also increased the levels ... 2A,B). This result was confirmed using the calcium ionophore ionomycin. Treatment of α-synuclein-expressing cells with 100 nm ... ionomycin for 6 h led to a significant increase in the levels of extracellular α-synuclein (percentage secreted α-synuclein, ...http://www.jneurosci.org/content/30/20/6838
Cutting Edge: Identification of a Motile IL-17-Producing γδ T Cell Population in the Dermis | The Journal of Immunology
ionomycin. LN. lymph node. PLN. peripheral lymph node. RORγt. retinoic acid-related orphan receptor γt. WT. wild-type.. ... and 1 μg/ml ionomycin (I; EMD Biosciences) in brefeldin A (BD Biosciences), stained for surface Ags, treated with BD Cytofix ...http://www.jimmunol.org/content/186/11/6091
Inhibition of TCR Signaling by Herpes Simplex Virus | The Journal of Immunology
A and B, D-F, Mock and inactivated T cells were TCR stimulated with OKT3 or (C) stimulated with PMA plus ionomycin. Cells were ... Ionomycin causes activation of p38 and p42/44 mitogen-activated protein kinases in human neutrophils. Am. J. Physiol. 281: C350 ... ERK is phosphorylated in HSV-inactivated T cells stimulated with PMA and ionomycin. Treatment of T cells with the mitogen PMA ... PMA activates protein kinase C and ionomycin mobilizes calcium leading to the activation of ERK (49, 50), steps that are distal ...http://www.jimmunol.org/content/176/3/1825.full
Mucosal associated invariant T cells from human breast ducts mediate a Th17-skewed response to bacterially exposed breast...
However, whereas phorbol 12-myristate 13-acetate/ionomycin stimulation induced the production of both interferon-γ and IL-17 by ... On the basis of our analyses of MAIT cell responses to PMA/ionomycin stimulation (Fig. 3a-c), it was clear that the breast duct ... 5b, top panels). The PBMC-derived MAIT line B12, which produced IFN-γ but not IL-17A in response to PMA/ionomycin, showed only ... Total breast duct cells or PBMCs were stimulated with PMA and ionomycin, then stained them with MR1-5RU tetramer and anti-CD3 ...https://breast-cancer-research.biomedcentral.com/articles/10.1186/s13058-018-1036-5
Assays for Apoptosis and Autophagy-Section 15.5 | Thermo Fisher Scientific - CA
This ionomycin response can be blocked with cyclosporine A, a compound reported to prevent mitochondrial transition pore ... This ionomycin response can be blocked with cyclosporine A, a compound reported to prevent mitochondrial transition pore ... cells that have been loaded with calcein AM and CoCl2 can also be treated with a Ca2+ ionophore such as ionomycin (I24222, ... cells that have been loaded with calcein AM and CoCl2 can also be treated with a Ca2+ ionophore such as ionomycin (I24222, ...https://www.thermofisher.com/ca/en/home/references/molecular-probes-the-handbook/assays-for-cell-viability-proliferation-and-function/assays-for-apoptosis.html
Expansion of Human Peripheral Blood γδ T Cells using Zoledronate | Protocol (Translated to Italian)
Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation ... Espansione ex vivo di tumore cellule T reattive per mezzo di 1/Ionomycin Bryostatin e il Comune catena gamma Citochine ...https://www.jove.com/video/3182/espansione-del-sangue-periferico-umano-cellule-t-con-zoledronato?language=Italian
Activity-Dependent Cleavage of the K-Cl Cotransporter KCC2 Mediated by Calcium-Activated Protease Calpain | Journal of...
To test whether KCC2 is a calpain substrate in neurons, we incubated brain slices in the presence of ionomycin. Ionomycin ... C, D, Ionomycin triggers a decrease in KCC2 protein level. C, Incubation of brain slices in the presence of the Ca2+ ionophore ... 2002) Ionomycin-activated calpain triggers apoptosis. A probable role for Bcl-2 family members. J Biol Chem 277:27217-27226. ... Ionomycin assay of calpain activation.. To directly raise the intracellular Ca2+ concentration ([Ca2+]i), coronal rat brain ...http://www.jneurosci.org/content/32/33/11356.full
Impaired CD4 and CD8 Effector Function and Decreased Memory T Cell Populations in ICOS-Deficient Patients | The Journal of...
7⇑A). The RE values of MAF in CD4 T cells in response to PMA/ionomycin and TCR/CD28 in controls were 3.3 ± 1.4 and 4.4 ± 1.0, ... A and C, PBMCs were stimulated with PMA and ionomycin for 5 (IL-17) or 8 h (IFN-γ and IL-4). Cells were stained for ... To test this, we stimulated PBMCs with PMA/ionomycin and tested for intracellular IFN-γ, IL-4, or IL-17 in the CD4+CD45RO+ ... Purified CD4 T cells were stimulated with PMA/ionomycin for 4 h or anti-CD3/CD28 for 24 h, and the mRNA expression level of T- ...http://www.jimmunol.org/content/182/9/5515.long