Paul Sniegowski | Department of Biology
Genetic complementation analyses have shown that these high mutation rates are caused by defects in the MMR pathway. I am using ... Analysis of mutation rate data from fluctuation tests: Robust confidence intervals and hypothesis testing. Genetics, in ... Fluctuation test analysis programs (compiled for Windows) and source code from: Shaver, A.C. and P.D. Sniegowski. ... A test of the directed mutation hypothesis in Escherichia coli MCS2 using replica plating. Journal of Bacteriology 177:1119- ...https://www.bio.upenn.edu/people/paul-sniegowski
Adenoviral DNA replication: DNA sequences and enzymes required for initiation in vitro - CSHL Scientific Digital Repository
Adenoviruses Human Base Sequence Cell Nucleus DNA Viral DNA Directed DNA Polymerase Genes Viral Genetic Complementation Test ... dna dna polymerase radioisotope virus dna adenovirus dna replication dna sequence genetic engineering heredity nonhuman ...http://repository.cshl.edu/25106/
Molecular cloning and functional analysis of a blue light receptor gene MdCRY2 from apple (Malus domestica).
Genetic Complementation Test. Light. Malus / genetics*, growth & development, metabolism. Molecular Sequence Data. Plant ... the interaction between MdCRY2 and AtCOP1 using a yeast two-hybrid assay and a bimolecular fluorescence complementation assay. ...http://www.biomedsearch.com/nih/Molecular-cloning-functional-analysis-blue/23314496.html
Complementation in Zellweger syndrome: biochemical analysis of newly generated peroxisomes.
Genetic Complementation Test. Humans. Microbodies / metabolism*, ultrastructure. Oxidation-Reduction. Zellweger Syndrome / ... Since this disorder is genetically heterogeneous and several complementation groups are known, we were able to study the ... normalization of peroxisomal activity during the process of complementation. The restoration of catalase and dihydroxyacetone ...http://www.biomedsearch.com/nih/Complementation-in-Zellweger-syndrome-biochemical/1511996.html
Smyd3 methylates H4K5 in vitro and in vivo. (A) Methyla | Open-i
Genetic Complementation Test. *HeLa Cells. *Humans. *Methylation. *Mice. *Mice, Inbred C57BL. *Mice, Knockout ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC3368817_epi-7-340-g2&req=4
The CG12120 Gene Product Possesses NBAD Hydrolase and N | Open-i
Genetic Complementation Test. *Molecular Sequence Data. *Mutation. *Sequence Homology, Amino Acid. Related in: MedlinePlus ... To test whether CG12120 possesses these predicted activities, we produced recombinant CG12120 protein using a baculovirus ... To test whether CG12120 possesses these predicted activities, we produced recombinant CG12120 protein using a baculovirus ... This is, to our knowledge, the first molecular genetic characterization of NBAD hydrolase and carcinine hydrolase activity in ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC1285064_pgen.0010063.g004&req=4
Kinetics of SAM transport in E. coli. (A) Time course | Open-i
Genetic Complementation Test. *Kinetics. *Molecular Sequence Data. *S-Adenosylhomocysteine/metabolism. *Sequence Homology ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC3104491_mbo0031111070002&req=4
3D-structural models of archaeal THI4 family proteins c | Open-i
Genetic Complementation Test. Related in: MedlinePlus. © Copyright Policy - open-access Related In: Results - Collection ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC4215014_12866_2014_260_Fig2_HTML&req=4
Characterization of secreted HCV-LP produced in the pre | Open-i
Genetic Complementation Test. *Genotype. *Humans. *Microscopy, Electron, Transmission. *RNA, Viral/genetics. *Replicon ... Bottom Line: This closely resembled replicon-mediated NS2 trans-complementation, confirming that baculovirus expression of HCV ... Bottom Line: This closely resembled replicon-mediated NS2 trans-complementation, confirming that baculovirus expression of HCV ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC4202265_068932-f5&req=4
ZFIN Publication: Brand et al., 1996
Genetic Complementation Test; Mesoderm/pathology; Motor Neurons/pathology; Mutation*; Nervous System/embryology; Zebrafish/ ... We also find fusions of the ventral neurocranium for all midline mutants tested, which may reveal a hitherto unrecognized ...http://zfin.org/ZDB-PUB-970210-11
ZFIN Publication: Kramer et al., 2002
Enhancer Elements, Genetic. *Female. *Genetic Complementation Test. *Homozygote. *Molecular Sequence Data. *Mutation ... and four former isolates initially assigned to the cpt and pgy complementation groups. Overexpression analyses demonstrate that ...http://zfin.org/ZDB-PUB-021016-11
B. bacteriovorus AspRS aspartylates tRNAAsn.Aminoacylat | Open-i
Genetic Complementation Test. *Kinetics. *Substrate Specificity. *Transfer RNA Aminoacylation. © Copyright Policy Related In: ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC4206432_pone.0110842.g001&req=4
A nuclear magnetic resonance based approach to accurate functional annotation of putative enzymes in the methanogen...
For a number of case studies, additional experiments (such as in vivo genetic complementation) were performed to determine ... Here, we describe the use of nuclear magnetic resonance (NMR)-based ligand screening as a tool for testing functional ... The functional annotation of MA4265 was further tested using genetic complementation studies. The E. coli genome contains three ... Gene complementation. Genetic complementation of E. coli mutants by the MA genes was performed with E. coli deletion mutants ...https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-12-S1-S7
000646 - A/J
A/J inbred mice are widely used to model cancer and for carcinogen testing given their high susceptibility to carcinogen- ... Genetic complementation tests have shown allelism between the mdfw (modifier of deaf waddler) locus and the ahl locus. Further ... as confirmed by a genetic complementation test. Birth Defects Res A Clin Mol Teratol 76(8):574-9. PubMed: 16998816MGI: J:117594 ... In addition, a standard genetic test of allelism between clf1 and a Wnt9b targeted mutation demonstrated noncomplementation, ...https://www.jax.org/strain/000646
Protocols and Video Articles Authored by Joshua T. Gamse
Linkage analysis and genetic-complementation testing indicate that lor is an allele of tbx2b/fby (from beyond). fby was ... To test for developmental roles of scn8a, we knocked down Nav1.6a protein using antisense morpholinos. Na+ channel protein and ... To resolve the paradox that flhand tbx2b mutants have opposite phenotypes but have been placed in the same genetic pathway, we ... The zebrafish has proven to be a useful model to explore the genetic basis of neuroanatomical asymmetry in the developing ...https://www.jove.com/author/Joshua+T._Gamse
Pre-binding of small protein B to a stalled ribosome triggers trans-translation.
Blotting; Northern, Cell Division, Centrifugation; Density Gradient, Escherichia coli/metabolism, Genetic Complementation Test ...http://uu.diva-portal.org/smash/record.jsf?pid=diva2:102123
"In vitro CpG methylation increases the transformation efficiency of Bo" by Qiang Chen, Joshua Richard Fischer et al.
To test whether a methylation-sensitive restriction system contributes to poor B. burgdorferi transformability, shuttle ... The utility of in vitro methylation for the genetic manipulation of B. burgdorferi was exemplified by the ease of plasmid ... complementation of a B. burgdorferi B31 A3 BBK32 kanamycin-resistant (B31 A3 BBK32::Kan(r)) mutant, deficient in the expression ... as to hinder the genetic study of putative virulence factors. Two putative restriction-modification (R-M) systems, BBE02 ...https://escholarship.umassmed.edu/gsbs_sp/1402/
"Dominant negative mutator mutations in the mutS gene of Escherichia co" by Te-hui Wu and Martin G. Marinus
Genetic Complementation Test; Hydroxylamine; Hydroxylamines; Molecular Sequence Data; MutS DNA Mismatch-Binding Protein; * ... Mutagenesis; Nalidixic Acid; Nucleic Acid Heteroduplexes; Plasmids; *Point Mutation; Recombination, Genetic; Rifampin; Sequence ...https://escholarship.umassmed.edu/oapubs/663/
mediaTUM - Medien- und Publikationsserver
Genetic complementation tests using Pitx3 ( ak/+ ) mice confirmed eyl as a new allele of Pitx3 (Pitx3 ( eyl )). Sequencing of ... Genetic complementation tests using Pitx3 ( ak/+ ) mice confirmed eyl as a new allele of Pitx3 (Pitx3 ( eyl )). Sequencing of ...http://mediatum.ub.tum.de/1072936
Rmr6 Maintains Meiotic Inheritance of Paramutant States in Zea mays | Genetics
Genetic complementation tests between the ems97406 mutation and other mutations known to affect Pl′ were conducted using +/ ... Stock constructions and complementation tests:. Hand pollinations were used for all genetic crosses and detailed pedigree ... Genetic tests demonstrate that Rmr6 encodes a common component required for establishing paramutations at diverse maize loci. ... To test the role of Rmr6 in r1 paramutation, near isogenic R-r/R-r and R-st/R-st W22 lines were first pollinated by a single +/ ...http://www.genetics.org/content/171/2/725
Rb deficiency during Drosophila eye development deregulates EMC, causing defects in the development of photoreceptors and cone...
Because d09015 is homozygous lethal, we performed a genetic complementation test to determine whether d09015 is an emc allele. ... To test this, we would need to generate double mutant clones of rbf1 and emc. However, because emc itself is required for ... Genetic screen and identification of d09015. To sustain high levels of UAS-transgene expression in both early and late stages ... 2A). We tested whether mis-expression of emc is capable of recapitulating the eye phenotype generated by d09015 by taking ...http://jcs.biologists.org/content/124/24/4203
Genetic Analysis and Gene Mapping. Genetic complementation tests and analysis of dominance were performed as described ... Genetic complementation analysis of all pairwise combinations of mutants was performed. Based on the thermotolerance phenotype ... and the new alleles were tested (before backcrossing) in many of the assays, as discussed in the text. Further genetic and ... thermotolerance was tested in all three assays, using the defined hot1-1 mutant as a reference. When tested for hypocotyl ...http://www.plantphysiol.org/content/132/2/757
Arabidopsis hot Mutants Define Multiple Functions Required for Acclimation to High Temperatures | Plant Physiology
Genetic Analysis and Gene Mapping. Genetic complementation tests and analysis of dominance were performed as described ... Genetic complementation analysis of all pairwise combinations of mutants was performed. Based on the thermotolerance phenotype ... and the new alleles were tested (before backcrossing) in many of the assays, as discussed in the text. Further genetic and ... thermotolerance was tested in all three assays, using the defined hot1-1 mutant as a reference. When tested for hypocotyl ...http://www.plantphysiol.org/content/132/2/757.full
Vacuolar precursor proteins undergo maturation after in | Open-i
Genetic Complementation Test. *Intracellular Membranes/enzymology/metabolism. *Micropore Filters. *Qa-SNARE Proteins ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC2199724_JCB9904110.f3&req=4
A single ataxia telangiectasia gene with a product similar to PI-3 kinase.
Genetic Complementation Test. *Genetic Predisposition to Disease. *Heterozygote. *Humans. *Male. *Meiosis. *Molecular Sequence ... ATM, which has a transcript of 12 kilobases, was found to be mutated in AT patients from all complementation groups, indicating ... The disease is genetically heterogeneous, with four complementation groups that have been suspected to represent different ...https://publications.ncats.nih.gov/publications/7792600