*  Protocols and Video Articles Authored by Lixin Liu
... cardiac alpha-myosin heavy chain, and troponin I. GFP-positive cells in myocardium sectioned from MI hearts confirmed the ... but the function of this gene remains unclear. In this study, a real-time polymerase chain reaction-based assay was developed ... The eukaryotic expression plasmid pcDNA 3.1 (+)/r-Caspase-3 was constructed by rearrangement of the large subunit and small ... 1950). Dec, 2004 , Pubmed ID: 15585887 Lymphocyte CD44 interactions with hyaluronan localized on the endothelium have been ...
  https://www.jove.com/author/Lixin_Liu
*  Faculty
Regulation of B cell fate commitment and immunoglobulin heavy-chain gene rearrangements by Ikaros. Nature Immunology, 9(8), 927 ... Molecular pathways and mechanisms regulating the recombination of immunoglobulin genes during B-lymphocyte development. Adv Exp ... In addition to regulation of Immunoglobulin Heavy Chain loci, the nuclear envelope (NE) is also implicated in regulating, among ... Fundamental questions in gene expression of interest to the lab include:. Why are changes in gene expression required for ...
  http://biolchem.bs.jhmi.edu/Pages/Faculty.aspx?ra=2
*  Basic concepts in immunity and inflammation
The first re-arrangement entails the joining of the D segment to the J segment of the immunoglobulin heavy chain gene (IgH). ... T-lymphocyte ontogeny:. T-cell development occurs in the thymus. T-cell precursors, however, arrive at the thymus from the bone ... two heavy chains (H chains) of identical molecular weight up to 53,000 Daltons (D) and two light chains (L chains) having a ... These are composed of two separate polypeptide chains, the heavier (44-47 KDa) α chain and the lighter (12 KDa) β chain. The α ...
  http://periobasics.com/basic-concepts-in-immunity-and-inflammation.html
*  Application # 2011/0123542. CCL20-SPECIFIC ANTIBODIES FOR CANCER THERAPY - Patents.com
Single-chain Fvs are prepared by constructing a structural gene comprising DNA sequences encoding the heavy chain variable and ... including gene rearrangement, chain assembly, and antibody repertoire. Ample guidance for practicing such an approach is ... One can use the lymphocyte pool of humans as starting material for the construction of an antibody library. It is possible to ... the light chain variable domain being aligned with the variable domain of the heavy chain and the light chain constant domain ...
  http://patents.com/us-20110123542.html
*  Patente US20100092475 - Treatment method using egfr antibodies and src inhibitors and related ... - Google Patentes
Preferably, an antigen binding domain comprises an antibody light chain variable region (VL) and an antibody heavy chain ... gene amplification is associated with EGFR rearrangements with the most common mutation, the de2-7 EGFR (or EGFRvIII), ... Antibody heteroconjugates and bispecific antibodies for use in regulation of lymphocyte activity. ... is that structural portion of an antibody molecule comprised of light chain or heavy and light chain variable and hypervariable ...
  http://www.google.es/patents/US20100092475
*  Malignant Lymphomas Involving Lung and Pleura | SpringerLink
... immunoglobulin heavy chain genes of marginal zone cell lymphomas indicates an origin from different marginal zone B lymphocyte ... Rearrangements of the BCL6 gene in diffuse large cell non-Hodgkin's lymphoma. Blood. 1994;83(7):1757-9.PubMedGoogle Scholar ... Analysis of LAZ3 (BCL-6) status in B-cell non-Hodgkin's lymphomas: results of rearrangement and gene expression studies and a ... Brinker MG, Poppema S, Buys CH, Timens W, Osinga J, Visser L. Clonal immunoglobulin gene rearrangements in tissues involved by ...
  https://link.springer.com/chapter/10.1007/978-1-4419-0787-5_11
*  Bertil Glader | Stanford Medicine Profiles
... and the immunoglobulin gene rearrangement studies showed rearranged heavy chain genes with germ line light chain genes. ... In addition, the SUP-HD1 cell line, produced IFN-gamma, a T-lymphocyte-associated lymphokine. Based on these data, the SUP-HD1 ... demonstrated rearrangement of the immunoglobulin heavy chain and kappa light chain genes as well as the gene for the beta chain ... Because the beta chain TCR gene is in chromosome band 7q34, we searched for and report finding specific rearrangements of 7q34 ...
  https://med.stanford.edu/profiles/bertil-glader
*  AIDS-Related Lymphoma Treatment (PDQ®): Treatment - Health Professional Information [NCI]
AIDS-related lymphomas, though usually of B-cell origin as demonstrated by immunoglobulin heavy-chain gene rearrangement ... Most patients in these series had either mixed cellularity or lymphocyte-depleted Hodgkin lymphoma, expression of Epstein-Barr ... Severity of the underlying immunodeficiency (measured by CD4 lymphocyte count in peripheral blood). ... Severity of the underlying immune deficiency (as measured by the CD4 lymphocyte count). ...
  http://healthmedicinet.com/content/nci/ncicdr0000062940.html
*  MONOCLONAL ANTIBODIES AGAINST HEPATITIS C VIRUS CORE PROTEIN - Patent application
For example, the technology for expression of both heavy and light chain genes in E. coli is the subject of the PCT patent ... Briefly, to produce a human-human hybridoma, a human lymphocyte donor is selected. A donor who is known as infected with HCV ( ... genetic rearrangement and mutation for expression of a particular immunoglobulin. (See, e.g., Shapiro et al., Crit. Rev. ... is a first heavy chain variable domain, VD2 is a second heavy chain variable domain, C is a heavy chain constant domain, X1 is ...
  http://www.patentsencyclopedia.com/app/20120009196
*  Antibody - wikidoc
In these rapidly dividing cells, the genes encoding the variable domains of the heavy and light chains undergo a high rate of ... "Evidence for somatic rearrangement of immunoglobulin genes coding for variable and constant regions". Proc. Natl. Acad. Sci. U. ... Goding J. "Allotypes of IgM and IgD receptors in the mouse: a probe for lymphocyte differentiation". Contemp Top Immunobiol. 8 ... Heavy chain. For more details on this topic, see heavy chain.. There are five types of mammalian Ig heavy chain denoted by the ...
  http://ec2-184-73-211-184.compute-1.amazonaws.com/index.php/Immunoglobulin
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
B lymphocyte immunoglobulin heavy chain (IgH) class switch recombination (CSR) is a process wherein initially expressed IgM ... We outline efficient methods for generating gene deletions, gene replacements, and tagged genes by targeted insertion or ... Exchange events between these repetitive elements can lead to genome rearrangements, including translocations, that can disrupt ... complemented gene function, and/or tagged genes provides powerful strategies to address gene function, particularly if these ...
  https://www.jove.com/visualize/abstract/21085586/analysis-mice-lacking-dnasei-hypersensitive-sites-at-5-end-igh
*  انواع التحاليل الطبية - clinical biochemistry
B-cell Gene Rearrangement. 238. B-cell Ig Gene Rearrangement. 239. B-cell Immunoglobulin Gene Rearrangement. 240. B-type ... CD4 Lymphocyte Count. 383. CD4 Percent. 384. CD4/CD8 Ratio. 385. CD8 Lymphocyte Count. 386. CEA. 387. Celiac Disease Tests. 388 ... Heavy Metals. 771. Heavy Metals Panel. 772. Helicobacter pylori. 773. Hematocrit. 774. Hemoccult. 775. Hemoglobin. 776. ... Free Light Chains. 675. Free PSA. 676. Free T3. 677. Free T4. 678. Free Testosterone. 679. Free Urine Catecholamines. 680. Free ...
  https://ehab10f.blogspot.com/2016/10/clinical-biochemistry.html
*  Frontiers | Genomic Architecture may Influence Recurrent Chromosomal Translocation Frequency in the Igh Locus | Immunology
... regions and mutations in V gene exons (6-10). Studies indicate that non-Ig genes are mistargeted by AID (11, 12) and thereby ... Translocation breakpoints adjacent to the DH or JH gene segments form secondary to V(D)J recombination, a process that occurs ... Two forms of loops generate the chromatin conformation of the immunoglobulin heavy-chain gene locus. Cell (2011) 147:332-43. ... B) CSR promotes diversification of CH effector function while retaining the original V(D)J rearrangement. Within the mouse Igh ...
  https://www.frontiersin.org/articles/10.3389/fimmu.2013.00500/full
*  GenomeRef: 2013
The 1 Mbp IGH locus on chromosome 14q32.33 contains genes that encode for the heavy chain of immunoglobulin molecules that ... 2). This locus is even more complicated than the LRC given that the IGH genes are subject to somatic rearrangements, and ... attempts to reconcile the organization of the locus using B-lymphocyte derived material have been difficult. The GRC has now ... and the immunoglobulin heavy chain locus (IGH). These new paths have now been released as fix patches in GRCh37.p11. ...
  http://genomeref.blogspot.no/2013/
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  http://pd19.org/ebook/download-vitu-grammar-sketch
*  Aberrant immunoglobulin class switch recombination and switch translocations in activated B cell-like diffuse large B cell...
CSR is a recombination and deletion mechanism that juxtaposes a downstream Ig heavy chain (CH) segment to the rearranged VHDHJH ... BCL-6 represses genes that function in lymphocyte differentiation, inflammation, and cell cycle control. Immunity. 13:199-212. ... and rearrangements of the gene encoding Blimp-1 are recurrent in ABC DLBCLs but are not present in GCB DLBCLs (12, 13). Blimp-1 ... 53BP1 links DNA damage-response pathways to immunoglobulin heavy chain class-switch recombination. Nat. Immunol. 5:481-487. ...
  http://jem.rupress.org/content/204/3/633
*  Approach to lymphocytosis - Cancer Therapy Advisor
Serum free light chains, if elevated and skewed toward kappa or lambda, may also provide evidence for lymphocyte clonality. In ... For example, identification of clonal rearrangement of the immunoglobulin genes, as seen in B-cell leukemias/lymphomas, or ... Heavy Alcohol Intake in Early Life May Significantly Increase Odds of High-Grade Prostate Cancer ... The rate of change of the lymphocyte count can influence treatment decisions. For example, a lymphocyte doubling time of less ...
  http://www.cancertherapyadvisor.com/oncology/approach-to-lymphocytosis/article/619207/
*  Core-binding factor subunit beta
Environmental and chemotherapeutic agents induce breakage at genes involved in leukemia-causing gene rearrangements in human ... of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain ... This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding ... The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the ...
  https://pharos.nih.gov/idg/targets/Q13951
*  B cell repertoires in HLA-sensitized kidney transplant candidates undergoing desensitization therapy | Journal of Translational...
We developed an assay based on high-throughput DNA sequencing of the variable domain of the heavy chain of immunoglobulin genes ... Genomic rearrangement of variable (V), diversity (D) and joining (J) segments of immunoglobulin genes generates a diverse set ... Measurement and clinical monitoring of human lymphocyte clonality by massively parallel VDJ pyrosequencing. Sci Transl Med. ... The relative usage of different V-J gene combinations (44 V genes × 6 J genes = 264) was compared by determining the relative ...
  https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-017-1118-7
*  Ash A. Alizadeh, MD/PhD | Stanford Medicine Profiles
... by gene expression profiling using DNA microarrays and for the presence of ongoing mutations in their Ig heavy chain gene. All ... Each stage of lymphocyte differentiation can be defined by a characteristic gene expression signature. Genes that are co- ... FACTERA: a practical method for the discovery of genomic rearrangements at breakpoint resolution BIOINFORMATICS Newman, A. M., ... 427 genes), T-cells (222 genes), CD8+ T-cells (23 genes), granulocytes (411 genes), and lymphocytes (67 genes). No ...
  https://med.stanford.edu/profiles/arash-alizadeh
*  Lymph Nodes - Credo Reference
... and at the molecular level show monoclonal rearrangements of the immunoglobulin heavy and/or light chain genes, indicating ... M heavy chain antibodies. In addition, antigen-specific lymphocyte clones are retained and concomitantly become depleted from ... They may contain cytoplasmic immunoglobulin (μ, γ, α and occasionally δ heavy chain with either κ or λ light chain), but have ... α and μ heavy chains with κ and λ light chains). Tingible-body macrophages express lysosome markers such as CD68, while the ...
  https://search.credoreference.com/content/topic/lymph_nodes
*  Mucosa-associated lymphoid tissue (MALT) lymphoma: a practical guide for pathologists | Journal of Clinical Pathology
Analysis of immunoglobulin heavy chain gene rearrangement in myoepithelial sialadenitis by polymerase chain reaction. Am J Clin ... κB-a transcription factor that regulates the expression of genes involved in lymphocyte proliferation and survival.46,47 In ... McClure RF, Kaur P, Pagel E, et al. Validation of immunoglobulin gene rearrangement detection by PCR using commercially ... PCR for IGH gene rearrangement should be performed only when there is a lymphoid infiltrate morphologically suspicious of ...
  http://jcp.bmj.com/content/60/4/361
*  Download Precalculus Functions And Graphs Eleventh Edition With Cengagenow Printed Access Card
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  http://pd19.org/ebook/download-precalculus%3A-functions-and-graphs%2C-eleventh-edition-%28with-cengagenow-printed-access-card%29
*  Pediatric Posttransplant Lymphoproliferative Disease: Overview, Incidence, Risk Factors
Lymphocyte CD20 expression is of value on determining therapy choices. Determination of cell surface heavy and light chain Ig ... immunoglobulin gene-rearrangement, and virologic. The classification schemes have common features, including benign hyperplasia ... Although the EB virus can express up to 100 genes, in the posttransplant situation only 9-10 genes are expressed. The EB genome ... Expression of latency III genes and XBP1 gene are associated with worse outcomes. [34] ...
  https://emedicine.medscape.com/article/1013758-overview
*  AIDS-Related Lymphoma Treatment (PDQ®): Treatment - Health Professional Information [NCI] - North Kansas City Hospital, Kansas...
AIDS-related lymphomas, though usually of B-cell origin as demonstrated by immunoglobulin heavy-chain gene rearrangement ... Most patients in these series had either mixed cellularity or lymphocyte-depleted HL, expression of Epstein-Barr virus (EBV)- ... Severity of the underlying immunodeficiency (measured by CD4 lymphocyte count in peripheral blood). ... by polymerase chain reaction).[13,14] Intrathecal chemotherapy is usually considered for patients who are at higher risk of CNS ...
  https://www.nkch.org/patients-visitors/health-library/healthwise-document-viewer/?id=ncicdr0000062940