We also identified a set of expressed sequence tags (ESTs) representing syn2 from an EST collection derived from a normalized ... Changes in syn2a-expressing cells after song stimulation. A, B, The number of syn2a-expressing NCM cells that were gad65 ... We used the sequences derived from the PCR-amplified clones and from ESTIMA clones to assemble full-length transcript sequences ... In contrast, despite extensive searches of chicken and zebra finch genomic sequences with coding and noncoding sequences from ...http://www.jneurosci.org/content/28/46/11871
The full-length cDNA sequence of human and mouse ZNRF2 was determined by analyzing expressed sequence tag (EST) clones (wh57e11 ... ZNRF2 is highly expressed in the nervous system. ZNRF1 is expressed predominantly in the nervous system during development and ... These clones were sequenced completely, and the sequence information was combined with those present in GenBank (Unigene ... The full-length cDNA sequence of this transcript was determined by sequencing multiple EST clones identified by the search and ...http://www.jneurosci.org/content/23/28/9385
... reads and 0.7 million Expressed Sequence Tags (ESTs) generated by Sino-Danish Pig Genome Project, and 1 million miscellaneous ... and Sequence View that presents the complete information of raw sequences. What are pig consensus sequences?. One pig consensus ... One sequence, either read or EST, was arbitrarily anchored to its best-aligned human gene, but the sequence might also be ... After all the sequences are anchored, PHRAP was applied to assemble collected sequences for each exon. The resultant contigs ...http://pig.genomics.org.cn/faq.jsp
Identification of Candidate Marker Genes. To take advantage of the vast information of the expressed sequence tags databases ... The differentially expressed genes were ranked by sequence odds ratio. The genes with the highest sequence odds ratios were ... differentially expressed genes between two pools of samples provided that a sufficient number of expressed sequence tags ... The Digital Gene Expression Displayer program identified differentially expressed genes among 47,036 sequences in five lung ...http://clincancerres.aacrjournals.org/content/11/1/173
PHOREST: a web-based tool for comparative analyses of expressed sequence tag data
... Ahrén, Dag LU ; Troein, Carl LU ; Johansson ... Additionally, expressed sequence tag database information can be used for the construction of DNA microarrays and for the ... Additionally, expressed sequence tag database information can be used for the construction of DNA microarrays and for the ... Comparative analysis of expressed sequence tags is becoming an important tool in molecular ecology for comparing gene ...https://lup.lub.lu.se/search/publication/135646
ESTviewer: a web interface for visualizing mouse, rat, cattle, pig and chicken conserved ESTs in human genes and human...
The application takes human gene accession Ids or coordinates of genomic sequences as inputs and presents annotated gene ... with emphasis on mammalian and avian expressed sequence tags (ESTs) that are conserved in the human genome and alternatively ... with emphasis on mammalian and avian expressed sequence tags (ESTs) that are conserved in the human genome and alternatively ... The application takes human gene accession Ids or coordinates of genomic sequences as inputs and presents annotated gene ...https://www.semanticscholar.org/paper/ESTviewer-a-web-interface-for-visualizing-mouse-ra-Chen-Chuang/a62d425d04f1b74886cf64ba56da294401cabd00
Expressed sequence tag - Wikipedia
In genetics, an expressed sequence tag (EST) is a short sub-sequence of a cDNA sequence. ESTs may be used to identify gene ... Because of the way ESTs are sequenced, many distinct expressed sequence tags are often partial sequences that correspond to the ... Jun 1991). "Complementary DNA sequencing: expressed sequence tags and human genome project". Science. 252 (5013): 1651-6. doi: ... In an effort to reduce the number of expressed sequence tags for downstream gene discovery analyses, several groups assembled ...https://en.wikipedia.org/wiki/Expressed_sequence_tag
IJMS | Free Full-Text | Generation and Analysis of Expressed Sequence Tags (ESTs) from Halophyte Atriplex canescens to Explore...
Additionally, among the 343 unigenes sequences, 22 simple sequence repeats (SSRs) were also identified contributing to the ... In an evaluation of 343 valid EST sequences in the cDNA library, 197 unigenes were assembled, among which 190 unigenes (83.1% ... All the 343 EST sequences have been deposited in the dbEST GenBank under accession numbers JZ535802 to JZ536144. According to ... expressed sequence tags; salt stress tolerance; SSRs Atriplex canescens; cDNA library; expressed sequence tags; salt stress ...http://mdpi.com/1422-0067/15/6/11172/xml
Analysis of Expressed Sequence Tags From Two Starvation, Time-of-Day-Specific Libraries of Neurospora crassa Reveals Novel...
Therefore, it was decided to use an expressed sequence tag (EST) sequencing approach to address the question of clock ... 2000 Identification of differentially expressed genes in cardiac hypertrophy by analysis of expressed sequence tags. Genomics ... 20,000 sequences. Contig analysis allowed the identification of 445 unique expressed sequence tags (ESTs) and 986 ESTs present ... Analysis of Expressed Sequence Tags From Two Starvation, Time-of-Day-Specific Libraries of Neurospora crassa Reveals Novel ...http://www.genetics.org/content/157/3/1057
Chasing Migration Genes: A Brain Expressed Sequence Tag Resource for Summer and Migratory Monarch Butterflies (Danaus plexippus)
Here, we describe a brain expressed sequence tag (EST) resource to identify genes involved in migratory behaviors. A brain EST ... Of 9,484 unique sequences, 6068 had positive hits with the non-redundant protein database; the EST database likely represents ∼ ...http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0001345
Analysis of Codon Usage Patterns in Toxic Dinoflagellate Alexandrium tamarense through Expressed Sequence Tag Data
... tamarense CCMP 1598 for protein-coding sequences from 10865 expressed sequence tags (ESTs). We reconstructed a total of 4284 ... Dinoflagellate expressed sequence tag data indicate massive transfer of chloroplast genes to the nuclear genome. Protist. 2004; ... Insights into a dinoflagellate genome through expressed sequence tag analysis. BMC Genomics. 2005;6(1, article 80) [PMC free ... Tanikawa N, Akimoto H, Ogoh K, Chun W, Ohmiya Y. Expressed sequence tag analysis of the dinoflagellate Lingulodinium polyedrum ...http://pubmedcentralcanada.ca/pmcc/articles/PMC2967832/
A Comprehensive Expressed Sequence Tag Linkage Map for Tiger Salamander and Mexican Axolotl: Enabling Gene Mapping and...
Recently completed expressed sequence tag (EST) sequencing projects for two tiger salamander species, A. mexicanum (Haberman et ... Expressed sequence tag (EST) markers were developed for Ambystoma tigrinum tigrinum (Eastern tiger salamander) and for A. ... A Comprehensive Expressed Sequence Tag Linkage Map for Tiger Salamander and Mexican Axolotl: Enabling Gene Mapping and ... A Comprehensive Expressed Sequence Tag Linkage Map for Tiger Salamander and Mexican Axolotl: Enabling Gene Mapping and ...http://www.genetics.org/content/171/3/1161
"Expressed sequence tags from <i>Diabrotica virgifera virgifera</i> mid" by Blair D. Siegfried, N....
Of the remaining sequences, most predict proteins with either catalytic (62%) or binding (19%) functions, as expected for ... Here we describe a collection of 691 sequences from the Western corn rootworm midgut, 27% of which predict proteins with no ... proteins expressed in the insect midgut. The utility of this approach for the identification of targets for novel toxins is ... Expressed sequence tags from Diabrotica virgifera virgifera midgut identify a coleopteran cadherin and a diversity of ...https://digitalcommons.unl.edu/entomologyfacpub/74/
"Functional Analysis and Comparative Genomics of Expressed Sequence Tag" by Jing-Ke Weng, Milos Tanurdzic et al.
Results We sequenced 2181 Expressed Sequence Tags (ESTs) from a S. moellendorffii cDNA library. One thousand three hundred and ... thaliana tentative consensus sequences. We compared S. moellendorffii ESTs to the A. thaliana and Physcomitrella patens EST ... one non-redundant sequences were assembled, containing 291 contigs and 1010 singletons. Approximately 75% of the ESTs matched ... We sequenced 2181 Expressed Sequence Tags (ESTs) from a S. moellendorffii cDNA library. One thousand three hundred and one non- ...https://docs.lib.purdue.edu/biochempubs/3/
Analyses of expressed sequence tags of anther and anther-specific cDNA Clones in Nicotiana tabacum | Springer for Research &...
Expressed sequence tag (EST) analysis of tobacco ( Nicotiana tabacum) anthers was performed for 200 cDNA clones randomly chosen ... Expressed sequence tags of fruits, peels, and carpels and analysis of mRNA expression levels of the tagged cDNAs of fruits from ... Analysis of 176 expressed sequence tags generated from cDNA clones of hot pepper by single-path sequencing. J Plant Biol 41: ... Lim CO, Kim HY, Kim MG, Lee SI, Chung WS, Park SH, Hwang I, Cho MJ (1996) Expressed sequence tags of Chinese cabbage flower bud ...https://rd.springer.com/article/10.1007/BF03030503
A tree-structured index algorithm for expressed sequence tags clustering
Abstract Expressed sequence tags (ESTs) are complementary deoxyribonucleic acid (cDNA) fragments, which are reverse transcribed ... ESTs are a readily rich information source of complete expressed gene sequences. They reveal the type and number of genes being ... This enables efficient consensus sequences generation, which reveals underlying gene sequences and their possible alternative ... expressed in an organism. Joining ESTs into complete gene sequences is computationally expensive because they are numerous, ...http://wiredspace.wits.ac.za/handle/10539/6001?show=full
Survey of glycine-rich proteins (GRPs) in the Eucalyptus expressed sequence tag database
... .. ... Survey of glycine-rich proteins (GRPs) in the Eucalyptus expressed sequence tag database. ... Survey of glycine-rich proteins (GRPs) in the Eucalyptus expressed sequence tag database. ... GRPs specifically expressed in xylem, phloem, epidermis, anther tapetum and roots have been described. In this paper, the ...http://www.lume.ufrgs.br/handle/10183/23384
Analysis of expressed sequence tags from the ectoparasitic nematode Xiphinema index » Brill Online
Genes encoding a variety of proteases, which were shown using in situ hybridisation to be expressed in the gut, were also ... Approximately 1400 genes were sequenced, 70% from a cDNA library generated from dissected basal bulbs (containing the ... proteins were abundant in the bulb library and in situ hybridisation confirmed that one of these is expressed in the basal bulb ... We report the results of a small-scale expressed sequence tag project performed on the ectoparasitic nematode Xiphinema index. ...http://booksandjournals.brillonline.com/content/journals/10.1163/1568541054192180
Large-scale development of expressed sequence tag-derived simple sequence repeat markers and diversity analysis inArachisspp. |...
Large-scale development of expressed sequence tag simple sequence repeat (EST-SSR) markers was performed in peanut (Arachis ... Kantety RV, La Rota M, Matthews DE, Sorrells ME (2002) Data mining for simple sequence repeats in expressed sequence tags from ... Large-scale development of expressed sequence tag-derived simple sequence repeat markers and diversity analysis in Arachis spp. ... Large-scale development of expressed sequence tag simple sequence repeat (EST-SSR) markers was performed in peanut (Arachis ...https://link.springer.com/article/10.1007%2Fs11032-011-9604-8
IJMS | Free Full-Text | Flavonoid Biosynthesis Genes Putatively Identified in the Aromatic Plant Polygonum minus via Expressed...
Thus, large‑scale sequencing of P. minus cDNA libraries identified 4196 expressed sequences tags (ESTs) which were deposited in ... Sequence Analysis and Potentials of the Native RbcS Promoter in the Development of an Alternative Eukaryotic Expression System ...http://www.mdpi.com/1422-0067/13/3/2692/notes
Generation and analysis of expressed sequence tags (ESTs) for marker development in yam (Dioscorea alata L.) | BMC Genomics |...
Generation and analysis of expressed sequence tags (ESTs) for marker development in yam (Dioscorea alata L.). ... Sequence assembly, analysis and annotation. *a.. Preliminary sequencing data analysis: The Sanger sequences were analyzed using ... 454 sequence data analysis: The sequencing agency provided high quality raw reads as well as aligned EST sequences for three ... The vector sequences from each sequence were trimmed manually, Sequences less than 150 base pairs were removed and then ESTs ...https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-12-100
Large-scale development of expressed sequence tag-derived simple sequence repeat markers by deep transcriptome sequencing in...
The lack of simple sequence repeat (SSR) markers is a major obstacle in genetic... ... Large-scale development of expressed sequence tag-derived simple sequence repeat markers by deep transcriptome sequencing in ... Diversity analysis in Cannabis sativa based on large-scale development of expressed sequence tag-derived simple sequence repeat ... Construction and evaluation of cDNA libraries for large-scale expressed sequence tag sequencing in wheat (Triticum aestivum L ...https://rd.springer.com/article/10.1007/s11032-015-0399-x
Expressed sequence tag-derived polymorphic SSR markers for Fucus serratus and amplification in other species of Fucus - ePIC
Expressed sequence tag-derived polymorphic SSR markers for Fucus serratus and amplification in other species of Fucus ... Coyer, J. A. , Hoarau, G. , Beszteri, B. , Pearson, G. and Olsen, J. L. (2009): Expressed sequence tag-derived polymorphic SSR ...http://epic.awi.de/20000/
Expression profiling of salinity-alkali stress responses by large-scale expressed sequence tag analysis in Tamarix hispid,...
"Expression profiling of salinity-alkali stress responses by large-scale expressed sequence tag analysis in Tamarix hispid, ... Expressed sequence tags from a NaCl-treated Suaeda salsa cDNA library. Zhang, L; Ma, XL; Zhang, Q; Ma, CL; Wang, PP; Sun, YF; ... Expression profiling of salinity-alkali stress responses by large-scale expressed sequence tag analysis in Tamarix hispid. Gao ... A 2003 census of the candidates, a study of the distribution of expressed sequence tags in organs, and a web-based database ...https://www.deepdyve.com/lp/springer_journal/expression-profiling-of-salinity-alkali-stress-responses-by-large-9DujmDW0a0
Combinatorial depletion analysis to assemble the network architecture of the SAGA and ADA chromatin remodeling complexes |...
... which also detects the IgG tag in the TAP tag, allowing for simultaneous visualization of both the tag and the interacting ... This table contains the protein names, spectra names, scoring information, sequence information, and summary information for ... expressed and purified by affinity purification (Supplementary Tables S1 and S2). The proteins bound to the respective subunits ... TAP tag and Mat, a knockout strains, were obtained from Open Biosystems. Gene deletions in the TAP tag strains were carried out ...http://msb.embopress.org/content/7/1/503