Pulsed-field gel electrophoresis - Wikipedia
Gel electrophoresis Nonlinear frictiophoresis Kaufmann, Mary Elizabeth (1998). "Pulsed-Field Gel Electrophoresis". Molecular ... Pulsed field gel electrophoresis is a technique used for the separation of large DNA molecules by applying to a gel matrix an ... Pulsed-field gel electrophoresis (PFGE) is a method used to separate large segments of DNA using an alternating and cross field ... Pulsed-Homogeneous Orthogonal Field Gel Electrophoresis (PHOGE) The 90 degree angle orientation method, also known as Pulsed ...
Pulsed-field gel electrophoresis (PFGE) typing | BIONUMERICS
Store all your epidemiological info and gel images in one database. With powerful statistical tools, BIONUMERICS makes it easy ... Pulsed-field gel electrophoresis (PFGE) typing. Pulsed-field gel electrophoresis (PFGE) typing. Pulsed-field gel ... DNA fragments are separated during electrophoresis using an electrical field of alternating polarity, after which the ... The entire gel image pre-processing is contained in a powerful tab-based window, allowing easy access to re-edit the processing ...
Figure 2 - Automated Ribotyping and Pulsed-Field Gel Electrophoresis for Rapid Identification of Multidrug-Resistant Salmonella...
Dendrogram of unique pulsed-field gel electrophoresis patterns of Salmonella Newport. The 58 patterns represent all patterns ... Automated Ribotyping and Pulsed-Field Gel Electrophoresis for Rapid Identification of Multidrug-Resistant Salmonella Serotype ... Automated Ribotyping and Pulsed-Field Gel Electrophoresis for Rapid Identification of Multidrug-Resistant Salmonella Serotype ...
Facilities / Instrument,Pulsed-field gel electrophoresis - Environmental Biotechnology And Genomics Division / The Official...
Pulsed-field gel electrophoresis - Environmental Biotechnology And Genomics Division ... R&D Division Environmental Biotechnology And Genomics Division Facilities / Instrument Pulsed-field gel electrophoresis ... Pulsed-field gel electrophoresis It is a technique by which genomic DNA is isolated from the organism of interest followed by ...
Pulsed Field Gel Electrophoresis for Dairy Propionibacteria - Normandie Université
Pulsed Field Gel Electrophoresis for Dairy Propionibacteria. Methods in molecular biology, pp.265-275, 2015, ⟨10.1007/978-1- ...
Use of pulsed-field gel electrophoresis to link sporadic cases of invasive listeriosis with recalled chocolate milk - Research ...
Electrochemical Techniques - Electrophoresis, Gel, Pulsed-Field | CU Experts | CU Boulder
Pulsed field gel electrophoresis of chromosomal DNA reveals a clonal population structure to Bacillus thuringiensis that...
T1 - Pulsed field gel electrophoresis of chromosomal DNA reveals a clonal population structure to Bacillus thuringiensis that ... Pulsed field gel electrophoresis of chromosomal DNA reveals a clonal population structure to Bacillus thuringiensis that ... title = "Pulsed field gel electrophoresis of chromosomal DNA reveals a clonal population structure to Bacillus thuringiensis ... Pulsed field gel electrophoresis of chromosomal DNA reveals a clonal population structure to Bacillus thuringiensis that ...
CDC - 2013 Prevention Status Reports (PSR) - Food Safety - STLT Gateway
Pulsed-field gel electrophoresis, or PFGE, is a technique used to distinguish between strains of organisms at the DNA level. ... Increasing the speed of pulsed-field gel electrophoresis (PFGE) testing of reported Escherichia coli (E. coli) O157 cases ... Note: An erratum for the indicator entitled "Speed of pulsed-field gel electrophoresis (PFGE) testing of reported E. coli O157 ... Speed of pulsed-field gel electrophoresis (PFGE) testing of reported E. coli O157 cases. ...
Instituto del Agua - Universidad de Barcelona
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Pulsed-field gel electrophoresis-typing of isolates. Molecular genotyping of P. aeruginosa isolates was carried out by pulsed- ... Pulsed-field gel electrophoresis (PFGE) of Pseudomonas aeruginosa isolates from patient 4. The genomic DNA for the 15 P. ... Pulsed-field gel electrophoresis (PFGE) of Pseudomonas aeruginosa isolates from patient 2. The genomic DNA for the 15 P. ... Interpreting chromosomal DNA restriction patterns produced by pulse-field gel electrophoresis: criteria for bacterial stain ...
Dirofilariasis: Practice Essentials, Background, Pathophysiology
The Use of Pulsed Field Gel Electrophoresis to Compare and Identify Isolates of Streptococcus Equi Subspecies Zooepidemicus -...
Characterization of Alloiococcus otitidis strains isolated from children with otitis media with effusion by Pulsed-Field Gel...
Alloiococcus otitidis Pulsed-Field Gel Electrophoresis (PFGE) Otitis media middle-ear fluid molecular analysis pathogens ... otitidis isolates from patients with OME were investigated via Pulsed-Field Gel Electrophoresis (PFGE) typing method. Methods: ... of Alloiococcus otitidis strains isolated from children with otitis media with effusion by Pulsed-Field Gel Electrophoresis. ... of Alloiococcus otitidis strains isolated from children with otitis media with effusion by Pulsed-Field Gel Electrophoresis. ...
Genome rearrangements and pervasive meiotic drive cause hybrid infertility in fission yeast | eLife
Pulsed-field gel electrophoresis and Southern blot analyses. Request a detailed protocol Agarose DNA plugs were prepared and ... B) Pulsed-field gels separating Sp and Sk chromosomes and Southern blots of the gels probed with DNA from the indicated loci ... To observe DSB formation, we used pulsed-field gel electrophoresis (PFGE) of whole chromosomes in rad50S hybrid and control ... Ethidium bromide stained pulsed-field gel of rad50+ diploids at the given times after the induction of meiosis show that DSBs ...
EUR-Lex - 32010R0333 - EN - EUR-Lex
NHANES 2003-2004: Methicillin - Resistant Staphylococcus aureus (MRSA) Data Documentation, Codebook, and Frequencies
... strain typing by pulsed-field gel electrophoresis (PFGE) using SmaI enzyme; singleplex polymerase chain reaction (PCR) for ... strain-typing by pulsed-field gel electrophoresis (PFGE) using SmaI enzyme; singleplex polymerase chain reaction (PCR) for ... and strain-typing by pulsed-field gel electrophoresis (PFGE) using SmaI enzyme. ...
Binational outbreak of Guillain-Barré syndrome associated with Campylobacter jejuni infection, Mexico and USA, 2011 |...
Rapid pulsed-field gel electrophoresis protocol for subtyping of Campylobacter jejuni . Journal of Clinical Microbiology 2001; ... pulsed-field gel electrophoresis (PFGE) [Reference Ribot24], and multilocus sequence typing (MLST) [Reference Dingle25]. ... 30 000 tons of chicken manure and nearly 1 million tons of locally produced cattle manure are applied to YC fields annually [ ...
FoodCORE Center: Ohio | FoodCORE | CDC
Ohio increased the capacity for conducting DNA fingerprinting using pulsed-field gel electrophoresis (PFGE). Before FoodCORE, ...
High burden of clonal hematopoiesis in first responders exposed to the World Trade Center disaster | Nature Medicine
The restriction digested DNA is resolved by pulse field gel electrophoresis. The slice containing the FRA16D locus is ... f) Replication fork speed during the IdU pulse of SMARD (first 4 hours of pulsing) in Region 1 of CFS-FRA16D in wildtype (WT) ... k) Replication fork speed during the IdU pulse of SMARD (first 4 hours of pulsing) in Region 2 of CFS-FRA16D in wildtype (WT) ... Note: Aphidicolin, WTC-PM and Olaparib are present during IdU pulse. (f): Replication fork speed during the IdU pulse of SMARD ...
The histone methyltransferase SET8 is required for S-phase progression | Journal of Cell Biology | Rockefeller University Press
Pulsed-field gel electrophoresis 48 h after SET8 siRNA transfection, cells were treated with 5 μM aphidicolin (Fluka) and/or ... 4 C and S2 B) and pulsed-field gel electrophoresis (Figs. 4 D and S2 C). This is consistent with a critical role for Chk1 in ... S1 D) as well as by direct analysis of DNA strand breaks using pulsed-field gel electrophoresis (Fig. 4 D). Collectively, our ... DNA damage induced by SET8 depletion visualized by pulsed-field gel electrophoresis; normalized against mock aphidicolin-CEP. ...
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Pathogenicity of an emergent, ovine abortifacient Campylobacter jejuni clone orally inoculated into pregnant guinea pigs in:...
Pulsed-field gel electrophoresis of Campylobacter jejuni heep abortion isolates. Vet Microbiol 2006;115:237-242. ... Pulsed-field gel electrophoresis of Campylobacter jejuni heep abortion isolates. Vet Microbiol 2006;115:237-242. ... Pulsed-field gel electrophoresis typing of Campylobacter fetus subsp. fetus isolated from sheep abortions in New Zealand. N Z ... Pulsed-field gel electrophoresis typing of Campylobacter fetus subsp. fetus isolated from sheep abortions in New Zealand. N Z ...
Case-control meta-analysis of blood DNA methylation and autism spectrum disorder | Molecular Autism
4 Attributes and Application of Indicators | Indicators for Waterborne Pathogens | The National Academies Press
Currently, the most widely used library-dependent genotypic methods include pulsed-field gel electrophoresis (PFGE), ribotyping ... although other methods such as denaturing gradient gel electrophoresis (DGGE) are also being investigated (Simpson et al., 2002 ... Bernhard, A.E., and K.G. Field. 2000b. A PCR assay to discriminate human and ruminant feces based on host differences in ... Bernhard, A.E., and K.G. Field. 2000a. Identification of nonpoint sources of fecal pollution in coastal waters by using host- ...
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Dr Helen E Bryant | Medicine and Population Health | The University of Sheffield
WTS database | WHO FCTC
Whole genome molecular epidemiology of E.coli O157 isolates from humans, food and the environment | Asiantaeth Safonau Bwyd
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2). Clone inserts were sized by pulse-field gel electrophoresis as described previously (Geisler, 2002). Although we could not ... A, Bright-field view of the NM and underlying supporting cells. Black dashed regions delineate two HCs that are in focus. White ... The outlines of HCs and underlying supporting cells can be visualized in a bright-field image of the section (Fig. 6A). As ... At each step, RACE products were excised from agarose gels, extracted (Nucleotrap; Macherey-Nagel, Easton, PA), subcloned in ...