miR-29b Participates in Early Aneurysm Development in Marfan SyndromeNovelty and Significance | Circulation Research
elastin. Fbn1C1039G/+. transgenic Marfan mice. IκB. NF-κB inhibitor. IKK. IκB kinase. LNA. locked nucleic acid. LNA post. LNA ... elastin (ELN) levels in Fbn1C1039G/+ AS aorta are decreased because of reduced early elastogenesis and increased elastin ... AS aorta after elastin van Gieson staining demonstrated an overall decrease in the amount of medial elastin, as well as ... increased elastin deposition, and decreased elastin fragmentation (Figure 6H) in LNA-treated Fbn1C1039G/+ mice compared to ...http://circres.ahajournals.org/content/110/2/312
MicroRNA-181b Controls Atherosclerosis and Aneurysms Through Regulation of TIMP-3 and Elastin | Circulation Research
MicroRNA-181b Controls Atherosclerosis and Aneurysms Through Regulation of TIMP-3 and Elastin. Karina Di Gregoli, Nur Najmi ... We determined that miR-181b negatively regulates macrophage TIMP-3 expression and vascular smooth muscle cell elastin ... Moreover miR-181b inhibition greatly increased elastin and collagen expression, promoting a fibrotic response and subsequent ... MicroRNA-181b Controls Atherosclerosis and Aneurysms Through Regulation of TIMP-3 and Elastin ...http://circres.ahajournals.org/content/early/2016/10/18/CIRCRESAHA.116.309321
MicroRNA miR145 Regulates TGFBR2 Expression and Matrix Synthesis in Vascular Smooth Muscle CellsNovelty and Significance |...
CNN indicates calponin1; COL1A1, collagen I; ELN, elastin; FN1, fibronectin; and MMP, matrix metalloproteinases; and n.s., not ... such as elastin, collagens, and the matrix crosslinking genes, Lox and Lox1.41,42 We examined the expression of these matrix ... elastin (Abcam, ab77804), calponin1 (Sigma, C2687), SM α-ACTIN (ACTA2; Sigma, 1A4), tubulin (TUBB2A; Sigma, T7816), and GAPDH ( ...http://circres.ahajournals.org/content/116/1/23
Heparin-Binding Epidermal Growth Factor-Like Growth Factor Signaling in Flow-Induced Arterial Remodeling | Circulation Research
C, Different animal from B showing ROS and elastin autofluorescence (green) 36 hours after ligation to demarcate intima for ... Green indicates elastin; blue, nuclear DAPI staining. Mitochondrial ROS was also not increased in the right carotid artery. ...http://circres.ahajournals.org/content/102/10/1275
Rapid accumulation of elastin and collagen in the aortas of sheep in the immediate perinatal period. | Circulation Research
Rapid accumulation of elastin and collagen in the aortas of sheep in the immediate perinatal period.. M P Bendeck, B L Langille ... Elastin content increased by 66%, and collagen increased by 57%. The pronounced increase in wall tissue accumulation near birth ... Thoracic aortic elastin content increased by 41%, and collagen content increased by 49% in approximately 1 week, between 140 ... Rapid accumulation of elastin and collagen in the aortas of sheep in the immediate perinatal period. ...http://circres.ahajournals.org/content/69/4/1165
Trophic Effect of Norepinephrine on Arterial Intima-Media and Adventitia Is Augmented by Injury and Mediated by Different α1...
Arrows in D indicate interrupted elastin lamellae; lumen arrowheads in E, presumed monocyte/macrophages; arrowheads in media in ...http://circres.ahajournals.org/content/89/9/815
Fibulin-4 Deficiency Results in Ascending Aortic Aneurysms | Circulation Research
In the Fbln4GKO aorta, numerous elastin globules are seen in the mutant vessel (arrow in b). At higher magnification, disarray ... Fibulin-5 is an elastin-binding protein essential for elastic fibre development in vivo. Nature. 2002; 415: 168-171. ... Fibulin-4 is an elastin-binding ECM protein.. Congenital loss of fibulin-4 results in severe disruption of elastic fibers and ... It has been shown that the aorta lacking elastin exhibits increased SMC proliferation and changes in SMC organization.18 We ...http://circres.ahajournals.org/content/106/3/583.long
Characterization of the Mouse Aortic Carboxypeptidase-Like Protein Promoter Reveals Activity in Differentiated and...
Sp1 regulates elastin transcription.45,46⇓ However, different from ACLP, the elastin promoter is not activated by Sp3 in ... The expression of ACLP is similar in vivo to elastin, which is required for proper blood vessel morphogenesis and structure.44 ... Elastin is an essential determinant of arterial morphogenesis. Nature. 1998; 393: 276-280. ... Transcriptional regulation of the elastin gene by insulin-like growth factor-I involves disruption of Sp1 binding: evidence for ...http://circres.ahajournals.org/content/90/6/728.long
Smad2 and Myocardin-Related Transcription Factor B Cooperatively Regulate Vascular Smooth Muscle Differentiation From Neural...
Elastin synthesis in arterial smooth muscle cell culture. J Cell Biol. 1976;68:411-419. ... VSMCs have been shown to produce elastin, the major component of the elastic fibers.50 It is unknown, however, how Smad2 ... Elastin). B, Higher magnification of H&E-stained vessel wall showed less VSMCs, thinner wall, and distorted and thinner elastic ... The sections were stained with hematoxylin and eosin for structural observation or Elastica van Gieson for elastin. For ...http://circres.ahajournals.org/content/113/8/e76
Discoidin Domain Receptor 1 (Ddr1) Deletion Decreases Atherosclerosis by Accelerating Matrix Accumulation and Reducing...
... mice stained with VVG to detect elastin. E, Quantification of the percentage of plaque area that stained positive for elastin ... Measurement of the percentage of plaque area stained for elastin revealed that elastin content was significantly greater in ... Ddr1−/−;Ldlr−/− plaques exhibited accelerated deposition of fibrillar collagen and elastin at 12 weeks compared with Ddr1+/+; ... Elastin accumulation in lesions was assessed by staining sections with VVG. Thick black elastic fibers were more prominent in ...http://circres.ahajournals.org/content/102/10/1202.full
S100A12 Links to Thoracic Aortic Aneurysms | Circulation Research
These included increased aortic thickening, elastin degradation, and fibrosis. Tissue extracts from transgenic mice had ...http://circres.ahajournals.org/content/106/1/13
Transient Exposure of Neonatal Female Mice to Testosterone Abrogates the Sexual Dimorphism of Abdominal Aortic AneurysmsNovelty...
Movat's pentachrome stain for elastin (black), collagen (yellow), and smooth muscle (red). Black arrows indicate severe elastin ... Our previous results demonstrated focal medial elastin degradation was an early event in AAA formation in adult male ApoE−/− ... Previous studies examining temporal changes in aneurysmal pathology with AngII infusion demonstrated focal elastin degradation ... In both genotypes, AAAs exhibited typical characteristics of pronounced adventitial thrombus, elastin degradation within the ...http://circres.ahajournals.org/content/110/11/e73
Plasminogen Is a Critical Determinant of Vascular Remodeling in Mice | Circulation Research
Sections are stained for elastin with Verhoeff-van Gieson stain.. Fibrinogen Deficiency Restores Vascular Remodeling Potential ...http://circres.ahajournals.org/content/87/2/133
Heparanase Alters Arterial Structure, Mechanics, and Repair Following Endovascular Stenting in Mice | Circulation Research
We mechanical tested the remaining elastin networks and found that the heparanase transgenic mice had elastin with dramatically ... To examine mechanical properties of the elastin networks, we digested aortae in 0.1 N NaOH at 75°C for 1 hour. The solution was ... The aortae were stained with hematoxylin/eosin or with an elastin Movat stain as previously described.15 Arterial thickness, ... This procedure has been shown to remove cellular components and extracellular matrix but leave the elastin network intact.19 ...http://circres.ahajournals.org/content/104/3/380
Targeted Disruption of the Matrix Metalloproteinase-9 Gene Impairs Smooth Muscle Cell Migration and Geometrical Arterial...
A, Collagen distribution 14 days after ligation in the remodeling arteries of WT and MMP-9 KO arteries, revealed by elastin ... Our finding suggests that MMP-9, known to degrade short collagens and elastin, may also participate directly or indirectly to ... Top, Characteristic morphology of elastin stained cross sections (bar=50 μm). Bottom, Morphometric and statistical analyses of ...http://circres.ahajournals.org/content/91/9/852.full
Loss of Elastic Fiber Integrity and Reduction of Vascular Smooth Muscle Contraction Resulting From the Upregulated Activities...
... the decreased contractility in MFS TA might be attributable to reduction of elastin, as SMCs from mice lacking elastin failed ... A critical role for elastin signaling in vascular morphogenesis and disease. Development. 2003; 130: 411-423. ... Elastin is the major component that confers elastic and viscoelastic properties on the aorta over most of the physiological ... 18 The broken elastin array is highly prone to calcification,17 further reducing arterial elasticity. In the clinical ...http://circres.ahajournals.org/content/101/5/512
Proteoglycans in Atherosclerosis and Restenosis | Circulation Research
Hinek A, Mecham RP, Keeley F, Rabinovitch M. Impaired elastin fiber assembly related to reduced 67-kD elastin-binding protein ... 127,128 Elastin peptides have been shown also to regulate ASMC proliferation and migration.129 In fact, elastin has been used ... Disruption of elastin synthesis by targeting the promoter and first exon of the tropoelastin gene126 leads to subendothelial ... Elastin is an essential determinant of arterial morphogenesis. Nature. 1998; 393: 276-280. ...http://circres.ahajournals.org/content/94/9/1158
Drilling for Oxygen | Circulation Research
Gelatinase B, denoted MMP-9, can break down the core proteins of proteoglycans and elastin. Together, the MMPs (now numbering ... elastin, microfibrillar proteins, proteoglycans, and other constituents of the surrounding extracellular matrix (Figure 1C). ...http://circres.ahajournals.org/content/89/3/195
Differential Role of G Protein-Coupled Receptor Kinase 5 in Physiological Versus Pathological Cardiac HypertrophyNovelty and...
Right coronary artery becomes stiffer with increase in elastin and collagen in right ventricular hypertrophy. J Appl Physiol ( ...http://circres.ahajournals.org/content/117/12/1001
Receptor-Interacting Protein Kinase 3 Contributes to Abdominal Aortic Aneurysms via Smooth Muscle Cell Necrosis and...
n=5 or 7. D, Cross sections of harvested aortae were stained for Verhoeff-Van Gieson (VVG) in which elastin fibers are stained ... Representative images in Figure 2B demonstrate elastin fragmentation and SMC paucity in Rip3+/+ aortae on day 14 after elastase ... Depletion of medial smooth muscle cells (SMCs), along with inflammation and elastin degradation, is a major histological ... elastin fragmentation and SMC depletion in the Rip3+/− donor aortae were absent (Figure 3D). These results suggest that ...http://circres.ahajournals.org/content/116/4/600
Increased Neointima Formation in Cysteine-Rich Protein 2-Deficient Mice in Response to Vascular Injury | Circulation Research
Verhoeff's staining for elastin (black) was performed on sections from (A) WT (Csrp2+/+) and (B) Csrp2−/− mice. Representative ... Verhoeff's staining for elastin (black) was performed on sections from (C) Csrp2+/+ and (D) Csrp2−/− mice. Representative ... Verhoeff's elastin stain of WT (C) and Csrp2−/− (D) mouse aortas reveal elastic layers (black) delineating the medium (Med), ... Verhoeff's elastin stain of control femoral arteries revealed the endothelial layer (Figure 4A) abutting the internal elastic ...http://circres.ahajournals.org/content/97/12/1323
Table of Contents - February 01, 1990, 66 (2) | Circulation Research
Alterations in elastin and collagen related to the mechanism of progressive pulmonary venous obstruction in a piglet model. A ...http://circres.ahajournals.org/content/66/2
β-Arrestin-2 Deficiency Attenuates Abdominal Aortic Aneurysm Formation in MiceNovelty and Significance | Circulation Research
Arrow indicates breakage in the elastin layer F, AngII-treated apoE−/−/βarr2−/− and (G) saline-treated apoE−/−/βarr2+/+ mice. ... Proteolytic degradation within the vessel wall mediated largely by MMPs is a common feature of AAAs, wherein elastin ... the elastin fibers in the aortas of AngII-treated apoE−/−/βarr2−/− mice appeared intact (Figure 5F), similar to the aortas of ... elastin) stain for histological analysis or with antibodies against COX-2, macrophage marker protein Mac-3, or p-ERK1/2 for ...http://circres.ahajournals.org/content/112/9/1219.long
Matrix Metalloproteinase-9 Is Necessary for the Regulation of Smooth Muscle Cell Replication and Migration After Arterial...
For example, inactivation of the MMP-11 gene was shown to increase neointimal formation and elastin degradation after ...http://circres.ahajournals.org/content/91/9/845.full
Serine Protease Inhibitor Serp-1 Strongly Impairs Atherosclerotic Lesion Formation and Induces a Stable Plaque Phenotype in...
Elastin staining of the cryosections showed no difference in lesional elastin content (4.8±1.1% in the control group versus 4.7 ... Sections were stained for collagen, using Picrosirius Red (Direct red 80) and for elastin, using an Accustain elastin staining ...http://circres.ahajournals.org/content/93/5/464