*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... The Swimmeret System of Crayfish: A Practical Guide for the Dissection of the Nerve Cord and Extracellular Recordings of the ... Characterization of Complex Systems Using the Design of Experiments Approach: Transient Protein Expression in Tobacco as a Case ... Such alloy material systems also provide advantages in comparison to traditional synthetic polymers due to the materials ...
  https://www.jove.com/visualize/abstract/24244318/drug-repositioning-kernel-based-integration-molecular-structure
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... This xenograft tumor model system is valuable for the pre-clinical in vivo evaluation of putative antitumor compounds. The 4T1 ... We describe an in vitro method to measure bladder smooth muscle contractility, and its use for investigating physiological and ... Three-dimensional co-culture systems are frequently used to explicate tumor-stroma interactions, including their role in drug ...
  https://www.jove.com/visualize/abstract/20175815/evaluation-inosin-5-monophosphate-dehydrogenase-activity-during
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... Here, we consider the zebrafish embryo as a useful in vivo system to perform such studies and present a protocol to measure in ... While the use of TMS has expanded to the study of many systems and processes during this time, the original application and ... They play a role in cellular physiological processes such as signalling, and trafficking1,2 but are also thought to be key ...
  https://www.jove.com/visualize/abstract/22328928/bace1-inhibition-induces-specific-cerebrospinal-fluid-amyloid-pattern
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... Newly released systems such as the VisualSonics Vevo2100 provide new tools for researchers to carefully and non-invasively ... Methods: An automatic or manual input in a single phase serves as an initialization from which the system starts to track the ... This system generates high resolution images and provides analysis capabilities similar to those used with human patients. ...
  https://www.jove.com/visualize/abstract/24023649/dependence-brain-intravoxel-incoherent-motion-perfusion-parameters-on
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... regulatory expression systems, and appropriate selectable markers. To circumvent these difficulties, model genetic systems in ... Endocrine System, Endocrine System Diseases, Orthotopic, mouse, anaplastic, thyroid, carcinoma, cancer, animal model ... An Ex vivo Culture System to Study Thyroid Development Authors: Anne-Sophie Delmarcelle, Mylah Villacorte, Anne-Christine Hick ...
  https://www.jove.com/visualize/abstract/21698151/the-loss-p53-activator-hipk2-is-responsible-for-galectin-3
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... The Bis-Tris gel system, an alternative to the conventional Laemmli system, generates better protein separation and resolution ... Cell Physiological Phenomena, mRNA translation, ribosomes, protein synthesis, genome-wide analysis, translatome, mTOR, eIF4E, ... Cells grown in 3D retain the important cues from the extracellular matrix and provide a physiologically relevant ex vivo system ...
  https://www.jove.com/visualize/abstract/23308187/scattering-mcf7-cells-heregulin-1-depends-on-mek-p38-map-kinase
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... can provide key insights into physical interactions present within colloidal systems. Colloidal systems are widely existent in ... Escherichia coli (E. coli) is the most widely used expression system for the production of recombinant proteins for structural ... Miniaturized microfluidic systems provide simple and effective solutions for low-cost point-of-care diagnostics and high- ...
  https://www.jove.com/visualize/abstract/23844256/mixing-time-effects-on-dispersion-performance-adhesive-mixtures-for
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... By trading off a decrease in resolution with a greater field of view, this imaging system can be used at the very least as a ... This phenomenon was due to CSC vaccine-induced humoral as well as cellular anti-CSC responses. In particular, splenocytes ... The current gold standard in clinical CTC detection and enumeration is the FDA-cleared CellSearch system (CSS). This manuscript ...
  https://www.jove.com/visualize/abstract/24223792/prognostic-value-targeted-inhibition-survivin-expression-esophageal
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... We developed a system whereby the endogenous BRCA1 protein in a cell was acutely depleted by RNAi targeting the 3'-UTR of the ... The Cre/lox system was first described in bacteriophages during the 1980's. Cre recombinase is a Type I topoisomerase that ... Since over-expression of APP, or a subdomain of it, might lead to an immediately lethal effect, we used the Cre-LoxP System to ...
  https://www.jove.com/visualize/abstract/24223114/an-over-expression-app-model-for-anti-alzheimer-disease-drug
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... Respiratory System, Eukaryota, Immune System Diseases, Respiratory Tract Diseases, Natural Science Disciplines, Life Sciences ( ... respiratory system, forced oscillation technique, respiratory system mechanics, airway hyperresponsiveness, flexiVent, lung ... Additionally, the nose is the entry point to the respiratory system and therefore one of the first sites to be exposed to any ...
  https://www.jove.com/visualize/abstract/24349408/arsenic-compromises-conducting-airway-epithelial-barrier-properties
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... system activity, and thus physiological stress, during the period of hormone incorporation. Because human scalp hair grows at ... Gene delivery system for the hair follicle using viral vectors or liposomes for gene targeting to the various cell types in the ... These regions are crucial in establishing the blood system in the embryos and leads to the eventual movement of stem cells into ...
  https://www.jove.com/visualize/abstract/25360669/a-case-control-study-maternal-periconceptual-pregnancy-recreational
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... The system can be used to perform a variety of assays such as the testing of chemical inhibitors; ectopic expression of genes ... Such a system is described for the specific case of trachea regeneration, but it can be modified for other target organs. ... Oral glucose tolerance test was performed on db/db mice treated with CGA and soleus muscle was isolated for 2-deoxyglucose ...
  https://www.jove.com/visualize/abstract/22412912/chlorogenic-acid-stimulates-glucose-transport-skeletal-muscle-via
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... Two components of the Tet-Off gene expression system9 are used as a readout system: the tetracycline-controlled transactivator ... As water is pulled through the system, it experiences hydraulic resistance, creating tension throughout the system and a low ... These organic based electrolyte systems are viable at ambient temperatures, but require a cooling system to ensure that ...
  https://www.jove.com/visualize/abstract/21887290/rectification-of-the-water-permeability-in-cos-7-cells-at-22-10-and-0c
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... This is a convenient model system to directly explore BCL-2 family function because the protein and lipid components are ... This in vitro model system can be manipulated and applied to other mucosal surfaces. Inflammatory responses that involve ... Membrane depolarization and ion fluxes are events that have been studied extensively in biological systems due to their ability ...
  https://www.jove.com/visualize/abstract/23637791/a-novel-transport-mechanism-for-momp-chlamydophila-pneumoniae-its
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... Here we describe a model system that can be used to globally scale up or down astrocytic group I metabotropic glutamate ... We also describe the isolation of primary bovine corneal endothelial cells and its use as model system to assess Cx43- ... There is a need to design and manufacture new corneal biomaterial devices able to mimic in detail the physiological environment ...
  https://www.jove.com/visualize/abstract/26134986/delivery-molecules-into-human-corneal-endothelial-cells-carbon
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... This system is highly flexible and can detect Env conformational changes induced by soluble CD4 or cellular proteins. It ... This phenomenon was due to CSC vaccine-induced humoral as well as cellular anti-CSC responses. In particular, splenocytes ... Oral infection of specific-pathogen free mice induces a local inflammatory response resulting in destruction of tooth ...
  https://www.jove.com/visualize/abstract/21533061/mapping-binding-between-tetraspanin-molecule-sjc23-schistosoma
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... Developmental Biology, Issue 79, Eukaryota, Biological Phenomena, Cell Physiological Phenomena, C. elegans, cell culture, ... The central nervous system (CNS) of Drosophila larvae is complex and poorly understood. One way to investigate the CNS is to ... C. elegans is a powerful model system, in which genetic and molecular techniques are easily applicable. Until recently though, ...
  https://www.jove.com/visualize/abstract/19357781/cuti-1-novel-tetraspan-protein-involved-c-elegans-cuticle-formation
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... Figure 1 illustrates a scheme of our system. With the combination of these two systems, we locally recruited a Rac activator to ... Lifecycle modeling systems model the virus lifecycle under biosafety level 2 conditions; however, until recently such systems ... Two components of the Tet-Off gene expression system9 are used as a readout system: the tetracycline-controlled transactivator ...
  https://www.jove.com/visualize/abstract/19247479/unusual-armadillo-fold-human-general-vesicular-transport-factor
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... The Cold Stage System The cold stage assembly (Figure 2) consists of a set of thermoelectric coolers that cool a copper plate. ... Thereby, the system may be a valuable tool to identify agents/small molecules that are able to interfere with proteolytic ... The single axon of each class IV neuron projects into the ventral nerve cord of the central nervous system11 where they may ...
  https://www.jove.com/visualize/abstract/23935886/a-preliminary-study-of-the-heating-effect-of-the-tibetan-plateau
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... Both delivery systems are simple and effective once the surgical aptitude is achieved. They do not require any extensive ... They play a role in cellular physiological processes such as signalling, and trafficking1,2 but are also thought to be key ... Such cases call for the use of model systems such as giant vesicles, liposomes or supported lipid bilayers (SLBs). Arrays of ...
  https://www.jove.com/visualize/abstract/20976108/aav-mediated-gene-delivery-adult-gm1-gangliosidosis-mice-corrects
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... We then demonstrate the use of the DNA IQ system (Promega) to isolate DNA and showcase the utility of this method to amplify ... Our work is aimed toward significantly narrowing such lists by exploiting the advantages of a simple animal model system. While ... Additionally, traversing cellular barriers in the body is crucial for both oral delivery of therapeutic NCs into the ...
  https://www.jove.com/visualize/abstract/21054462/assessment-cyp2c19-genetic-polymorphisms-korean-population-using
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... The GR has been a particularly useful system for studying hsp90 because the receptor must be bound to hsp90 to have an open ... These data show that the simple devices we have developed can be applied to a variety of model systems to study several cell ... Using Xenopus oocytes as expression system, we determine the amount of Rb+ (Li+) transported into the cells by measuring ...
  https://www.jove.com/visualize/abstract/21347375/efficient-nuclear-transport-structurally-disturbed-cargo-mutations
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... Intranasal administration is a method of delivering therapeutic agents to the central nervous system (CNS). It is non-invasive ... Xenopus laevis egg extract is a well-characterized, robust system for studying the biochemistry of diverse cellular processes. ... In order to design a better model system for these applications, phenotypically wild-type cortical astrocytes and neural stem ...
  https://www.jove.com/visualize/abstract/19771166/in-vivo-turnover-tau-app-metabolites-brains-wild-type-tg2576-mice
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... Characterization of Complex Systems Using the Design of Experiments Approach: Transient Protein Expression in Tobacco as a Case ... Cytokine signaling system analyses require the use of sensitive and reproducible multiplexed techniques. We use quantitative ... With the advent of molecular biological techniques, the chick has become a useful system in which to study gene regulation and ...
  https://www.jove.com/visualize/abstract/23185611/thermal-chemical-ph-induced-denaturation-multimeric-galactosidase
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Digestive System and Oral Physiological Phenomena, Biological Phenomena, Cell Physiological Phenomena, drug delivery systems, ... To elucidate the underlying molecular mechanisms, a novel in vitro co-culture model system was established, in which medium ... Jared reveals that the symbiosis exhibited by this system is multi-layered and involves not only a relationship between the ... Inhibitory neurons act in the central nervous system to regulate the dynamics and spatio-temporal co-ordination of neuronal ...
  https://www.jove.com/visualize/abstract/22511959/a-mid-cretaceous-origin-sociality-xylocopine-bees-with-only-two