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*  Influence of Viscosity in Fluid Atomization with Surface Acoustic Waves
Digital Microfluidics Combined with Surface Acoustic Wave Nebulization for Hydrogen-Deuterium Exchange Measurements. Journal of ... Our measurements show a linear decrease of the static contact angle on the sputter- deposited SiO2 surface with increasing ... An extraction unit was used to collect the aerosol above the measurement spot in order to prohibit fluid condensation on the ... The measurement point density used here was 2.1 points/wavelength in y-direction and 500 µm in x-direction. Fluid contact ...
  http://www.scirp.org/Journal/PaperInformation.aspx?paperID=71077
*  Super Spy variants implicate flexibility in chaperone action | eLife
Crosslinking and hydrogen-deuterium exchange measurements suggest that this hydrophobic region is involved in client protein ... Hydrogen-deuterium exchange. Hydrogen-deuterium exchange of Spy and Spy-Im7 complex was performed using top-down ECD-FTICR-MS, ... Hydrogen-deuterium exchange identifies Spy residues involved in client binding. Hydrogen-deuterium exchange at individual ... To ascertain the effects of client protein binding on deuterium exchange, we compared the level of hydrogen-deuterium exchange ...
  https://elifesciences.org/articles/01584
*  Faculty Collaboration Database - Brian Smith PhD
Deuterium Exchange Measurement. *Enzyme Activators. *Enzyme Inhibitors. *Enzymes and Coenzymes. *Histone Code ...
  https://fcd.mcw.edu/?module=faculty&func=view&name=Brian_Smith_PhD&id=5575
*  Different inhibition of Gβγ-stimulated class IB phosphoinositide 3-kinase (PI3K) variants by a monoclonal antibody. Specific...
Hydrogen-deuterium exchange coupled to MS measurements. Hydrogen-deuterium exchange coupled to MS (HDX-MS) analyses of PI3Kγ in ... In order to get insight into the antibody-p110γ interface, hydrogen-deuterium exchange coupled to MS (HDX-MS) measurements were ... 2015) Applications of hydrogen/deuterium exchange MS from 2012 to 2014. Anal. Chem. 87:99-118, doi:10.1021/ac5040242, pmid: ... 2013) Using hydrogen/deuterium exchange mass spectrometry to define the specific interactions of the phospholipase A2 ...
  http://www.biochemj.org/content/469/1/59
*  MS Wil GmbH - Deurion SAWN interface
... digital microfluidics combined with surface acoustic wave nebulization for hydrogen-deuterium exchange measurements. J ...
  https://www.mswil.com/sawn
*  Cyclic AMP Analog Blocks Kinase Activation by Stabilizing Inactive Conformation: Conformational Selection Highlights a New...
... amide deuterium exchange measurements and collisional cross sectional areas of molecules from ion mobility mass spectrometry ... Amide H/D exchange mass spectrometry shows that apo RA is highly dynamic and highlights clear differences in deuterium exchange ... All reported deuterium exchange values were corrected for a 32.7% back exchange by multiplying the raw centroid values by a ... This is completely consistent from both amide exchange and x-ray crystallography, whereby increased deuterium exchange together ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC3047156/
*  Destabilization of apoprotein is insufficient to explain Cu,Zn-superoxide dismutase-linked ALS pathogenesis. - PubMed - NCBI
Deuterium Exchange Measurement. *Humans. *Mass Spectrometry. *Metals/metabolism. *Mutation/genetics. *Superoxide Dismutase/ ... Global H/D exchange kinetics of WT and A4V apo-SOD1S-S under native and nonnative conditions. (A) H/D exchange kinetics for A4V ... The H/D exchange properties of the mutants were also wide-ranging: the MBR mutant apoproteins exhibited H/D exchange kinetics ... The results of H/D exchange compared with those of DSC for 12 ALS mutant SOD1S-S apoproteins. TMs as determined by DSC are ...
  https://www.ncbi.nlm.nih.gov/pubmed/16020530
*  Two cross-reactive monoclonal antibodies recognize overlapping epitopes on Neisseria meningitidis factor H binding protein but...
The antigenic regions targeted by the two mAbs were accurately mapped by hydrogen-deuterium exchange mass spectrometry (HDX-MS ... Deuterium Exchange Measurement, Epitope Mapping, Epitopes, Genetic Variation, Humans, Mass Spectrometry, Meningococcal ... The antigenic regions targeted by the two mAbs were accurately mapped by hydrogen-deuterium exchange mass spectrometry (HDX-MS ... MenB vaccine, hydrogen/deuterium exchange mass spectrometry, mAb synergy, meningococcal antigen, Antibodies, Monoclonal, ...
  https://www.neuroscience.ox.ac.uk/publications/445779
*  NMR paper NMR-detected hydrogen exchange and molecular dynamics simulations provide structural - BioNMR
NMR-detected hydrogen exchange and molecular dynamics simulations provide structural insight into fibril formation of prion ... NMR-detected hydrogen-deuterium exchange measurements, and molecular dynamics simulations. The fibrils contain approximately 50 ... NMR-detected hydrogen exchange and molecular dynamics simulations provide structural NMR-detected hydrogen exchange and ... Related Articles NMR-detected hydrogen exchange and molecular dynamics simulations provide structural insight into fibril ...
  http://www.bionmr.com/forum/journal-club-9/nmr-detected-hydrogen-exchange-molecular-dynamics-simulations-provide-structural-10056/
*  ABI Elite ESI-QqTOF Mass Spectrometry System - Natalie Ahn
It is also used extensively for proteomics applications, including hydrogen-deuterium exchange measurements to map protein- ... mass spectrometer capable of rapid scanning and high accuracy mass measurements, and a Waters ultra-high pressure liquid ...
  http://grantome.com/grant/NIH/S10-RR026641-01
*  Nalle Jonsson's group
Measurement of hydrogen/deuterium exchange at amide groups by NMR is frequently used for characterization of the dynamics in ...
  http://www.ifm.liu.se/chemistry/molbiotech/nalle_jonssons_group/
*  Publikationen | Max-Planck-Institut für biophysikalische Chemie
Chevelkov, V.; Giller, K.; Becker, S.; Lange, A.: Measurement of backbone hydrogen-deuterium exchange in the type III secretion ...
  http://www.mpibpc.mpg.de/publikationen?seite=3
*  Mass Spectrometry Facility | cbc.arizona.edu
This instrument is also used to perform gas-phase hydrogen/deuterium exchange (HDX) measurements in order to distinguish ... Accurate mass measurements are available in the reflecton mode with ± 3 ppm accuracy. Tandem MS/MS (TOF-TOF) is used for ...
  https://cbc.arizona.edu/facilities/mass_spec
*  mass spectrometry study of hydrogen/deuterium exchange
Mass spectroscopy based measurement of the rate and/or extent of the hydrogen/deuterium exchange. [ PMID. :. 18948593 ] ... Mass spectroscopy based measurement of the rate and/or extent of the hydrogen/deuterium exchange. ...
  https://www.ebi.ac.uk/ols/ontologies/mi/terms?obo_id=MI%3A0944
*  Revealing the secrets of proteins: A triple-nuclear and multi-dimensional high-resolution NMR spectroscopy study
NMR studies, including dynamic measurements and hydrogen-deuterium exchange, in combination with NMR, circular dichroism and ... fluorescence measurements at stepwise increasing temperatures revealed that the repeat is rigid at room temperature but that ...
  http://bora.uib.no/handle/1956/6236
*  Protein & RNA Folding and Methods development - IBS - Institut de Biologie Structurale - Grenoble / France
... or the measurement of fast hydrogen-deuterium exchange rates in partially unstructured native states or molten globular states ... However, multidimensional NMR requires long measurement times that are exponentially increasing with the dimensionality of the ...
  http://www.ibs.fr/research/research-groups/biomolecular-nmr-spectroscopy-group-paul-schanda/team-protein-folding-and-methods/article/protein-rna-folding-and-methods?lang=en
*  Department of Chemistry : News Archive - Durham University
... which involved pKa measurements of around 20 triazolium ions from the kinetics of deuterium exchange. Triazolium ions are used ... to establish a PhD student exchange program with a consortium of South African universities, coordinated by the University of ... electrostatic potential in order to allow the user to evaluate local as well as global changes caused by the residues exchange ... determination of the collision properties of ground-state molecules in a fully state-selective way with precision measurements ...
  https://www.dur.ac.uk/chemistry/news/news_archive/
*  Hydrogen-deuterium exchange - Wikipedia
"Electron transfer dissociation facilitates the measurement of deuterium incorporation into selectively labeled peptides with ... deuterium exchange mass spectrometry can determine the overall deuterium content of molecules which have undergone H/D exchange ... Hydrogen-deuterium exchange (also called H-D or H/D exchange) is a chemical reaction in which a covalently bonded hydrogen atom ... Undesired deuterium-hydrogen exchange is referred to as back-exchange and various methods have been devised to correct for this ...
  https://en.wikipedia.org/wiki/Hydrogen%E2%80%93deuterium_exchange
*  Analysis of Overlapped and Noisy Hydrogen/Deuterium Exchange Mass Spectra | SpringerLink
Noisy and overlapped mass spectrometry data hinder the sequence coverage that can be obtained from hydrogen deuterium exchange ... the precision of deuterium uptake measurements was improved for nearly every peptide examined. The improved processing ... H/D exchange Overlap Bimodal Deconvolution Deuterium Conformational dynamics Binomial Electronic supplementary material. The ... Guttman, M., Scian, M., Lee, K.K.: Tracking hydrogen/deuterium exchange at glycan sites in glycoproteins by mass spectrometry. ...
  https://link.springer.com/article/10.1007%2Fs13361-013-0727-5
*  Localizing Carbohydrate Binding Sites in Proteins Using Hydrogen/Deuterium Exchange Mass Spectrometry | SpringerLink
The application of hydrogen/deuterium exchange mass spectrometry (HDX-MS) to localize ligand binding sites in carbohydrate- ... Time-resolved HDX-MS measurements were performed on the free and ligand-bound forms of CTB5, Stx1B5, and TcdA-A2. A summary of ... Peptide-column interactions and their influence on back exchange rates in hydrogen/deuterium exchange-MS. J. Am. Soc. Mass ... Hamuro, Y., Coales, S.J., Morrow, J.A., Molnar, K.S., Tuske, S.J., Southern, M.R., Griffin, P.R.: Hydrogen/deuterium-exchange ( ...
  https://link.springer.com/article/10.1007%2Fs13361-015-1263-2
*  Structures and organization of adenovirus cement proteins provide insights into the role of capsid maturation in virus entry...
The propeptide interactions with the base of the hexons concur with recent hydrogen-deuterium exchange mass spectrometry ... studies (18) as well as with affinity measurements between VI and the hexon (32). Moreover, although it was not reported by Liu ... in agreement with recent interpretations from hydrogen-deuterium exchange mass spectrometry studies (18). ...
  http://www.pnas.org/content/111/32/11715
*  Dietary Cholesterol Feeding Suppresses Human Cholesterol Synthesis Measured by Deuterium Incorporation and Urinary Mevalonic...
4) The flux of sterol from the free-exchange pool is small during the measurement period.29 In contrast, determination of the ... Deuterium incorporation relies on several assumptions: (1) Newly synthesized cholesterol enters the larger, free-exchange ... Deuterium Incorporation Methodology. Incorporation of deuterium into erythrocyte free cholesterol was determined by sequential ... During the final week of each diet, 0.7 g D2O was given per kilogram of body water and deuterium incorporation into the ...
  http://atvb.ahajournals.org/content/16/10/1222.long
*  ORBi: Browsing ORBi
With this aim, we will use pulse-labelling hydrogen/deuterium exchange experiments, in combination with 2D-NMR measurements, to ... Here we report the backbone resonance assignments as the requirement for further hydrogen/deuterium exchange studies. [less ▲] ... Circular dichroism measurements indicated that all five ficins were highly structured, both in term of secondary and tertiary ... D1 and D2 according to their sequence of elution from a cation-exchange chromatographic support. Following rapid fractionation ...
  http://orbi.ulg.ac.be/browse?type=author&value=Vandenameele,%20Julie%20p010795
*  Hydrogen isotope biogeochemistry - Wikipedia
Their ratios represent an exchange factor that can calibrate measurements to correct for hydrogen and deuterium swapping. For ... This rapid exchange is particularly problematic for measurements of bulk organic material with these functional groups because ... This enables the measurements of quadrupolar doublets for each nonequivalent deuterium atom. Thus reduces peak overlaps and ... Analyzing deuterium content at the compound level avoids problems from differences in exchange rates, simplifies sources and ...
  https://en.wikipedia.org/wiki/Hydrogen_isotope_biogeochemistry
*  Researchers at Sandia, Northeastern develop method to study critical HIV protein | EurekAlert! Science News
... and allow rapid exchange of the watery support layer for the hydrogen-deuterium exchange experiments. ... The neutron reflection measurements were performed at the Center for Neutron Research at the National Institute of Standards ... The local dynamics from hydrogen-deuterium exchange mass spectrometry are acquired for many small portions of the protein, ... Engen's forte is hydrogen-deuterium exchange mass spectrometry, a technique that measures the local structure and flexibility ...
  https://www.eurekalert.org/pub_releases/2016-08/dnl-ras080316.php