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*  3 - Endonuclease I - Enterobacteria phage T7 - 3 gene & protein
Participates also together with gp6 in the degradation of host chromosome to provide nucleotides for phage DNA synthesis. ... Junction-resolving enzyme that selectively binds and cleaves four-way (Holliday) DNA junctions present after viral genomic ... deoxyribonuclease IV (phage-T4-induced) activity Source: UniProtKB-EC. *DNA binding Source: UniProtKB-KW ... Morganella phage MmP1. Morganella phage vB_MmoP_MP2. Pasteurella phage PHB02. Pasteurella phage PHB01. Erwinia phage FE44. ...
  http://www.uniprot.org/uniprot/P00641
*  NAVER Academic > Search...
Bacterial Proteins, biosynthesis, DNA-(Apurinic or Apyrimidinic Site) Lyase, Deoxyribonuclease IV (Phage T4-Induced), Drug ... A global response induced in Escherichia coli by redox-cycling agents overlaps with that induced by peroxide stress.. 1989 B ... A global response induced in Escherichia coli by redox-cycling agents overlaps with that induced by peroxide stress.. J T ... start page previous page 1 2 3 4 5 6 7 8 9 10 next page end page ...
  https://academic.naver.com/search.naver?field=3&query=Journal+of+Bacteriology+171%EA%B6%8C+7%ED%98%B8
*  Deoxyribonuclease IV - Wikipedia
Deoxyribonuclease IV (phage-T4-induced) (EC 3.1.21.2, endodeoxyribonuclease IV (phage T4-induced), E. coli endonuclease IV, ... Phage T4 Deoxyriboendonuclease IV at the US National Library of Medicine Medical Subject Headings (MeSH) Lindahl T, Gally JA, ... phosphooligonucleotide end-products Deoxyribonuclease IV is a type of deoxyribonuclease that functions at AP-sites. Friedberg, ... I. Purification and properties of endonuclease II from T4 phage-infected Escherichia coli". J. Biol. Chem. 244 (22): 6182-6191 ...
  https://en.wikipedia.org/wiki/Deoxyribonuclease_IV
*  List of EC numbers (EC 3) - Wikipedia
... deoxyribonuclease I EC 3.1.21.2: deoxyribonuclease IV (phage-T4-induced) EC 3.1.21.3: type I site-specific deoxyribonuclease EC ... lambda-induced) EC 3.1.11.4: exodeoxyribonuclease (phage SP3-induced) EC 3.1.11.5: exodeoxyribonuclease V EC 3.1.11.6: ... deoxyribonuclease V EC 3.1.22.1: deoxyribonuclease II EC 3.1.22.2: Aspergillus deoxyribonuclease K1 EC 3.1.22.3: now EC 3.1. ... 21.7 EC 3.1.22.4: crossover junction endodeoxyribonuclease EC 3.1.22.5: deoxyribonuclease X EC 3.1.25.1: deoxyribonuclease ( ...
  https://en.wikipedia.org/wiki/List_of_EC_numbers_(EC_3)
*  Identification of genes induced by growth hormone in rat liver using cDNA arrays. - Nuffield Department of Orthopaedics,...
Other induced genes include those encoding a lactate transporter (MCT-1), an extracellular matrix remodelling enzyme, MTI-MMP ( ... In this study, we used cDNA array analysis to identify genes rapidly induced in the liver of GH-deficient dwarf rats following ... Deoxyribonuclease IV (Phage T4-Induced), Metalloendopeptidases, Carbon-Oxygen Lyases, Mitogen-Activated Protein Kinases, p38 ... Identification of genes induced by growth hormone in rat liver using cDNA arrays. Share Share Share Share ...
  https://www.ndorms.ox.ac.uk/publications/651289
*  List of MeSH codes (D08) - Wikipedia
... deoxyribonuclease iv (phage t4-induced) MeSH D08.811.277.352.335.350.300 --- dna restriction enzymes MeSH D08.811.277.352. ... deoxyribonucleases, type i site-specific MeSH D08.811.150.280.260 --- deoxyribonucleases, type ii site-specific MeSH D08.811. ... deoxyribonucleases, type i site-specific MeSH D08.811.277.352.355.325.300.260 --- deoxyribonucleases, type ii site-specific ... deoxyribonucleases, type ii site-specific MeSH D08.811.277.352.335.350.300.260.240 --- deoxyribonuclease bamhi MeSH D08.811. ...
  https://en.wikipedia.org/wiki/List_of_MeSH_codes_(D08)
*  Effect of Variations in the Conditions of DNA Synthesis upon the Accuracy of DNA Replication | SpringerLink
E. Freese, The difference between spontaneous and base analog induced mutations in phage T4, Proc. Natl.. Acad. Sci., U.S.A., ... M. Bittner, R. L. Burke, and B. Alberts, Purification of the T4 gene 32 protein free from detectable deoxyribonuclease ... E. Freese, The specific mutagenic effect of base analogs on phage T4, J. Mol. Biol., 1: 87 - 105 (1959).CrossRefGoogle Scholar ... Z. W. Hall and I. R. Lehman, An in vitrotransversion by a mutationally altered T4-induced DNA polymerase, J. Mol. Biol., 36: ...
  https://link.springer.com/chapter/10.1007/978-1-4613-2449-2_11
*  Michael Levitt's Profile | Stanford Profiles
STABILIZATION OF PHAGE-T4 LYSOZYME BY ENGINEERED DISULFIDE BONDS PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED ... PERIODICITY OF DEOXYRIBONUCLEASE-I DIGESTION OF CHROMATIN SCIENCE Prunell, A., Kornberg, R. D., Lutter, L., Klug, A., Levitt, M ... Here we report four single particle cryo-electron microscopy (cryo-EM) structures of Mm-cpn, an archaeal group II chaperonin, ... Saccharide binding induces a change primarily in Ser 55 and Gln 56 to a conformation identical in all five copies. Thus, ...
  https://profiles.stanford.edu/michael-levitt
*  Disabling Cas9 by an anti-CRISPR DNA mimic | Science Advances
High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat. Biotechnol. 31, 822-826 (2013).. ... The deployment of DNA mimics to inhibit a DNA binding protein is an elegant solution that is often deployed in the phage-host ... The amplified DNA and the plasmid pJH376 were subject to enzyme digestion for 1 hour at 37°C and ligated with T4 DNA ligase ( ... All the soft masks used in refinement were generated via a four-step script edited by X. Li (Tsinghua University School of ...
  http://advances.sciencemag.org/content/3/7/e1701620.full
*  Patent US5856090 - DNA-methylase linking reaction - Google Patents
Phage display accomplishes this linkage by engineering the phage to display a small peptide on their coats. The phage contains ... Second, in addition to the mutation-inducing variations described in U.S. Pat. No. 4,683,195, other mutation-inducing PCR ... The sequence contains four Nae I methylase recognition sites.. III. PLASMID-POLYPEPTIDE DETERMINANT CONJUGATES. Another aspect ... The ligation reaction is preferably catalyzed by the enzyme T4 DNA ligase, although other ligases are known, such as E. coli ...
  http://www.google.com/patents/US5856090?dq=6861155
*  Patente US6025194 - Nucleic acid sequence of senescence asssociated gene - Google Patentes
The four subunits are linked by disulfide bridges into two dimers, which are joined to each other by noncovalent bonds. Copper ... When a protein or fragment of a protein is used to immunize a host animal, numerous regions of the protein may induce the ... Bacteriophage antibody display libraries may also be screened for phage able to bind peptides and proteins of the invention ... 0.05 μl of T4 gene 32 protein, and water. The PCR is performed for 25 cycles at 94 degrees C. for 45 sec., 60 degrees C. for 1 ...
  http://www.google.es/patents/US6025194
*  ASMscience | DNA Mismatch Repair
A dedicated site-specific endonuclease, MutH, is present, andMutL has no endonuclease activity; four exonucleases can ... Bromouracil-induced mutagenesis. (A) Mutation frequency of induced mutant strains as a function of bromouracil concentration ... Four-factor genetic crosses in the λ cI gene. The configurations of the two crosses between am6 and am321 are shown. In ... Very short patch mismatch repair in phage lambda: repair sites and length of repair tracts. Genetics 114:1041-1060. [PubMed]. ...
  http://www.asmscience.org/content/journal/ecosalplus/10.1128/ecosalplus.7.2.5
*  Microbiology MSII Temple Piggot Flashcards - Cram.com
double-stranded DNA, but some phage have single-stranded DNA, and others RNA. Ex. Escherichia coli phage T2, T4 and λ all have ... Mix A and B in the presence of deoxyribonuclease and as a control mix. them in its absence. If no recombinants can be found ... Bacterial RNA polymerase consists of a core of four protein subunits, α2ββ'. A fifth protein. subunit, σ (sigma) binds to the ... 5. Plasmid Free conjugative transposon- It induces bacteria to conjugate without any plasmid. The mechanism is not understood, ...
  http://www.cram.com/flashcards/microbiology-msii-temple-piggot-491449
*  Construction of recB-recD genetic fusion and functional analysis of RecBDC fusion enzyme in Escherichia coli | BMC Biochemistry...
Exonuclease activity was tested by T4, T4 gene 2- and P2 phages. Gene 2 protein binds to the ends of T4 DNA and protects T4 DNA ... Half the culture was not induced but was kept for future use. The other half was induced by incubating at 42°C for 15 min, then ... We tested four predictions: [1] the fusion enzymes cannot bind specifically to dsDNA ends and lack all enzyme activities; [2] ... Eichler DC, Lehman I: On the role of ATP in phosphodiester bond hydrolysis catalyzed by the RecBC deoxyribonuclease of ...
  https://bmcbiochem.biomedcentral.com/articles/10.1186/1471-2091-9-27
*  METHODS AND COMPOSITIONS FOR MODULATING HEPATOCYTE GROWTH FACTOR ACTIVATION - Patent application
... lambdoid phage display systems (WO 95/34683; U.S. Pat. No. 5,627,024), T4 phage display systems (Ren et al., Gene, 215: 439 ( ... 0176]To select for an antibody, binding oligopeptide or binding organic small molecule which induces cell death, loss of ... The inhibitor concentrations giving 50% inhibition (IC50) were calculated by fitting the data to a four parameter regression ... ribonuclease or a DNA endonuclease such as a deoxyribonuclease; DNase). [0152]For selective destruction of the tumor, the ...
  http://www.patentsencyclopedia.com/app/20090075317
*  US20100279283A1 - Method for molecular biology applications - Google Patents
T4 Polynucleotide kinase (PNKase): (from T4 bacteriophage) * *catalyzes the transfer of a phosphate from ATP to the 5′ end of ... Endonuclease IV: * *The Endonuclease IV recognizes apurinic/apyrimidinic (AP) sites of dsDNA and cleaves the phosphodiester ... Source: E. coli cells with a cloned gene 2 of Bacillus subtilis phage phi29 Applications: rolling circle amplification (RCA); ... b). The enhanced processivity induced by crowding was clearly apparent with the long M13 PCR assay (. FIG. 17. c). The addition ...
  https://patents.google.com/patent/US20100279283
*  RECOMBINANT BACTERIOPHAGE AND METHODS FOR THEIR USE - Patent application
These genes are homologues to the phage T4 gp38 (SEQ ID NO:32), which is known to be responsible for proper folding of the T4 ... and other P2-like phages (Nakayama et al., 2000). Pyocin production is induced by DNA damage (Matsui et al., 1993) and is ... includes the specificities of the other four. The receptors for the other four R-types form two branches, or families of ... Optionally, deoxyribonuclease was added at 1 unit per 40 ml, and the lysate was incubated at 37° C. for 15-30 minutes to ...
  http://www.patentsencyclopedia.com/app/20130184183
*  Indian Patents. 258133:C-MET ANTIBODIES
Phage(mid) display (also referred to herein as phage display in some contexts) can be used as a convenient and fast method for ... iv ) HVR-H1 comprising sequence D1 -D10, wherein D1 -D10 is GYTFTSYWLH (SEQ ID NO:4). (v) HVR-H2 comprising sequence E1 -E18, ... Cells are usually induced for about 12-50 hours, although longer or shorter induction time may be used.. To improve the ... 2400 U T4 ligase, and 30 U T7. polymerase for 3 h at room temperature.. The filled in product was analyzed on Tris-Acetate-EDTA ...
  http://www.allindianpatents.com/patents/258133-c-met-antibodies
*  TS23 Alpha-Amylase Variants With Altered Properties - Patent application
8 μL T4 DNA Ligase buffer (Invitrogen Cat. no. 46300-018), 25 μl distilled, autoclaved water and 1 μL T4 DNA Ligase, 1 unit/μL ... Additional liquefaction inducing enzymes may also be added. [0464]As used herein, the term "primary liquefaction" refers to a ... A four site, 81-member CCL was created by making all combinations of three possibilities at each site: wild-type, arginine, or ... 0089]A "host strain" or "host cell" is an organism into which an expression vector, phage, virus, or other DNA construct ...
  http://www.patentsencyclopedia.com/app/20100048446
*  Immune Response
11.6 The famous Kleinschmidt electron micrograph of phage T4 DNA extruded from the capsid. Before this photograph was made, ... It is relatively easy to determine that the poliovirus capsid is made up of just four proteins, and that the four capsid ... Most virus-induced diseases have low or only moderate mortality rates. Obviously, if a virus's mortality rate is too high, ... The data were obtained as follows Nuclease sensitivity was measured by the ability of deoxy-ribonuclease DNase or ribonuclease ...
  https://www.americorpshealth.biz/immune-response/
*  Human Anti-NGF Neutralizing Antibodies as Selective NGF Pathway Inhibitors - Patent application
0334]The four binding peaks of 4D4 mapped to the N-terminus, C-terminus, internal domains, as well as loops L2 and L4 in NGF as ... 0031]a) inhibits NGF induced survival in an in vitro neutralization assay with an IC50 of about 1×10-9 M or less; [0032]b) has ... 0112]The term "transduction" is used to refer to the transfer of genes from one bacterium to another, usually by a phage. " ... 4,710,473) by: (a) destroying the two endogenous NdeI restriction sites by end filling with T4 polymerase enzyme followed by ...
  http://www.patentsencyclopedia.com/app/20100034818
*  Polypeptides having endoglucanase activity and polynucleotides encoding same - Patent application
0274] Production of a substance from cellulosic material typically requires four major steps. These four steps are pretreatment ... 0328] The double-stranded cDNAs were recovered by centrifugation at 13,000×g and blunt-ended in 30 μl of T4 DNA polymerase ... Other methods that can be used include error-prone PCR, phage display (e.g., Lowman et al., 1991, Biochem. 30: 10832-10837; U.S ... 25: 53-65), or a reactor with intensive stirring induced by an electromagnetic field (Gusakov, A. V., Sinitsyn, A. P., Davydkin ...
  http://www.patentsencyclopedia.com/app/20120331587
*  The organization, expression, function and evolution of some essential genes from the hyperthermophilic eubacterium thermotoga...
The phage library was screened by plaque hybridization with the 2.2 kb XbaI-EcoRI fragment from plasmid pUC-TB4. Phage DNA from ... iv Table of Contents Abstract^ ii Table of Contents ^ iv List of Tables ^ viii List of Figures ^ ix Abbreviations ^ xi ... The expression of the T. maritima NusG protein from plasmid pPD1077 induced by the addition of IPTG to mid-log phase cells is ... Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature, 227: 680 ...
  https://open.library.ubc.ca/cIRcle/collections/ubctheses/831/items/1.0098821
*  Polypeptides Having Lysozyme Activity and Polynucleotides Encoding Same - Patent application
The unit determination was measured by lysis induced change in turbididy of a bacterial culture suspended in buffer. [0398] Non ... Other methods that can be used include error-prone PCR, phage display (e.g., Lowman et al., 1991, Biochemistry 30: 10832-10837 ... iv) the full-length complement thereof; or (v) a subsequence thereof; under medium to very high stringency conditions. ... bacteriophage T4 lysozyme (GH24), Sphingomonas flagellar protein (GH73) and Chalaropsis lysozymes (GH25). Lysozymes from the ...
  http://www.patentsencyclopedia.com/app/20140325711