*  ASMscience | Genetic Exchange
Transformation is defined as the mechanism by which exogenous DNA is taken up by bacteria and the DNA becomes heritable. In a ... The use of conjugation as a mechanism for DNA delivery may have the advantage of bypassing restriction barriers that could ... Conjugation may provide an important method for performing genetic manipulations in strains resistant to transformation. ... inhibit genetic exchange by natural transformation. Several potential barriers exist to genetic exchange for H. pylori. ...
  http://www.asmscience.org/content/book/10.1128/9781555818005.chap28
*  Protocols and Video Articles Authored by Nguyen Thi Le Thuy
Expression of a Cryptic Secondary Sigma Factor Gene Unveils Natural Competence for DNA Transformation in Staphylococcus Aureus ... is able to develop natural competence for transformation by DNA. We previously showed that a novel staphylococcal secondary ... which requires the expression of SigH-controlled competence genes. Additionally, using DNA from the N315 MRSA strain, we ... aureus cells producing active SigH become competent for transformation by plasmid or chromosomal DNA, ...
  https://www.jove.com/author/Nguyen_Thi+Le+Thuy
*  Protocols and Video Articles Authored by Kazuya Morikawa (Translated to Swedish)
Expression of a Cryptic Secondary Sigma Factor Gene Unveils Natural Competence for DNA Transformation in Staphylococcus Aureus ... is able to develop natural competence for transformation by DNA. We previously showed that a novel staphylococcal secondary ... DNA-topology assays using the cell extract showed that the extracts from the Deltafis and Topo I-/DNA gyrase-overexpressing ... Nevertheless, the main competence regulators and a series of orthologous genes that form the competence machinery are present ...
  https://www.jove.com/author/Kazuya_Morikawa?language=Swedish
*  Frontiers | Modeling of the ComRS Signaling Pathway Reveals the Limiting Factors Controlling Competence in Streptococcus...
In conclusion, we propose that the level of ComR production is one important factor that could explain competence heterogeneity ... comR overexpression in both strains was shown to induce higher competence levels with deregulated kinetics patterns during ... concentration is the main limiting factor for the level of comX expression and controls the kinetics of spontaneous competence ... concentration is the main limiting factor for the level of comX expression and controls the kinetics of spontaneous competence ...
  https://www.frontiersin.org/articles/10.3389/fmicb.2015.01413/full
*  Staphylococcus aureus - Wikipedia
"Expression of a cryptic secondary sigma factor gene unveils natural competence for DNA transformation in Staphylococcus aureus ... Natural genetic transformation is a reproductive process involving DNA transfer from one bacterium to another through the ... Further studies suggested that the development of competence for natural genetic transformation may be substantially higher ... S. aureus was found to be capable of natural genetic transformation, but only at low frequency under the experimental ...
  https://en.wikipedia.org/wiki/Staphylococcus_aureus
*  Structure and mechanism of the PilF DNA transformation ATPase from Thermus thermophilus | Biochemical Journal
... twitching motility and DNA transformation. DNA transformation, also known as competence, is a process by which bacteria can ... TtPilF-DNA complex retained DNA (top panel, Figure 3C), compared with polyAT DNA alone (bottom panel, Figure 3C). The ATPase ... Structure and mechanism of the PilF DNA transformation ATPase from Thermus thermophilus. Richard F. Collins, Darin Hassan, ... thermophilus can take up DNA with high efficiency and has been shown to have a broad DNA specificity [3]. DNA translocation and ...
  http://www.biochemj.org/content/450/2/417
*  SAUSA300 RS11115 - AureoWiki
Cellular processesCellular processesDNA transformationcompetence protein ComEA helix-hairpin-helix repeat region (TIGR00426; ... Cellular processesCellular processesDNA transformationcomEA protein (TIGR01259; HMM-score: 40.9) ... Genetic information processingTranscriptionDNA-dependent RNA polymeraseDNA-directed RNA polymerase (TIGR00448; EC 2.7.7.6; HMM- ... no clan definedHTH_44; Helix-turn-helix DNA-binding domain of SPT6 (PF14641; HMM-score: 10.8) ...
  http://aureowiki.med.uni-greifswald.de/SAUSA300_RS11115
*  Transformation (genetics) - Wikipedia
suggested that competence for transformation probably evolved as a DNA damage response. Logarithmically growing bacteria differ ... Competence is specifically induced by DNA damaging conditions. For instance, transformation is induced in Streptococcus ... "Low-level pilin expression allows for substantial DNA transformation competence in Neisseria gonorrhoeae". Infection and ... transformation is increased by UV light, a DNA damaging agent. In Helicobacter pylori, ciprofloxacin, which interacts with DNA ...
  https://en.wikipedia.org/wiki/Transformation_(genetics)
*  SACOL RS08170 - AureoWiki
Cellular processesCellular processesDNA transformationDNA internalization-related competence protein ComEC/Rec2 (TIGR00361; HMM ...
  http://aureowiki.med.uni-greifswald.de/SACOL_RS08170
*  NWMN 1449 - AureoWiki
Cellular processesCellular processesDNA transformationDNA internalization-related competence protein ComEC/Rec2 (TIGR00361; HMM ...
  http://aureowiki.med.uni-greifswald.de/NWMN_1449
*  Transporter Classification Database - Wikipedia
Na+-translocating Pyrophosphatase family 3.A.11 The Bacterial Competence-related DNA Transformation Transporter (DNA-T) Family ... 3.A.12 The Septal DNA Translocator (S-DNA-T) Family 3.A.13 The Filamentous Phage Exporter (FPhE) Family 3.A.14 The Fimbrilin/ ... Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family 3.A.8 The Mitochondrial Protein Translocase (MPT) Family ... Family 1.D.46 The DNA Nanopore (DnaNP) Family 1.D.47 The Pore-forming Synthetic Cyclic Peptide (PSCP) Family 1.D.48 The Pore- ...
  https://en.wikipedia.org/wiki/Transporter_Classification_Database
*  Bacterial natural transformation by highly fragmented and damaged DNA | PNAS
... closely related competence genes in Acinetobacter sp. strain BD413 and Thermus thermophilus HB27. Appl Environ Microbiol 67(7): ... E) Diagram of the ancient DNA experiment. Woolly mammoth DNA was used as donor DNA for natural transformation of the hisC::′ ... The data confirmed that transformation by short DNA was associated with DNA replication; in ,97% of transformants, the DNA ... Natural transformation experiments were performed as described in ref. 47 and SI Text, using as donor DNA chromosomal DNA, PCR ...
  http://www.pnas.org/content/110/49/19860
*  Archive | mSystems
In the cariogenic pathogen Streptococcus mutans, DNA transformation is regulated by the competence stimulating peptide XIP (Com ... Genetic competence provides bacteria with an opportunity to increase genetic diversity or acquire novel traits conferring a ... Transcriptional Profiling of the Oral Pathogen Streptococcus mutans in Response to Competence Signaling Peptide XIP ... The Baltic Sea Virome: Diversity and Transcriptional Activity of DNA and RNA Viruses ...
  http://msystems.asm.org/content/2/1
*  Neisseria gonorrhoeae - Wikipedia
"Competence for natural transformation in Neisseria gonorrhoeae: Components of DNA binding and uptake linked to type IV pilus ... The process in which recipient gonococci integrate DNA from neighboring gonococci into their genome is called transformation. ... have suggested that N. gonorrhoeae may replace DNA damaged in neutrophil phagosomes with DNA from neighboring gonococci. ... In addition to the ability to rearrange the genes it already has, it is also naturally competent to acquire new DNA, via its ...
  https://en.wikipedia.org/wiki/Neisseria_gonorrhoeae
*  Review. Bacterial type IV secretion mechanisms | Philosophical Transactions of the Royal Society B: Biological Sciences
2006 Functional and topological characterization of novel components of the comB DNA transformation competence system in ... 2002 Natural transformation in Helicobacter pylori: DNA transport in an unexpected way. Trends Microbiol. 10, 159-162. doi: ... mediate DNA uptake from the environment leading to bacterial transformation [6]. Both dissemination and transformation ... Natural transformation of H. pylori is exceptional because it involves a T4SS ComB [23,24]. Recent evidence indicates that DNA ...
  http://rstb.royalsocietypublishing.org/content/367/1592/1073
*  ASMscience | Genetic Recombination
This chapter talks about competence and transformation in their bacterial senses (competence to take up DNA, genetic ... DNA exonucleases, enzymes that hydrolyze phosphodiester bonds in DNA from a free end, play important cellular roles in DNA ... The purpose of this DNA unwinding is to provide nascent, single-stranded DNA (ssDNA) for the processes of DNA repair, ... showed that DNA damage did not induce competence in either H. influenzae or B. subtilis. Proponents of the transformation ...
  http://www.asmscience.org/content/concept/Entity/ASM/Microbiology/Microbial_Genetics_and_Evolution/Microbial_Genetics/Genetic_Recombination
*  DNA Transport across the Outer and Inner Membranes of Naturally Transformable Vibrio cholerae Is Spatially but Not Temporally...
The physiological state of natural competence for transformation allows certain bacteria to take up free DNA from the ... To better understand these mechanisms, we investigated the competence-mediated DNA transport in the naturally transformable ... which suggested the competence protein ComEA plays a role in the DNA uptake process across the outer membrane of Gram-negative ... DNA Transport across the Outer and Inner Membranes of Naturally Transformable Vibrio cholerae Is Spatially but Not Temporally ...
  https://infoscience.epfl.ch/record/201514
*  G-Links - a gene-centric link acquisition service
2 ko02044 M00429 Competence-related DNA transformation transporter 2 ko02042 Bacterial toxins 2 ko02022 M00502 GlrK-GlrR (amino ... 9 ko03400 M00260 DNA polymerase III complex, bacteria 9 ko03032 M00260 DNA polymerase III complex, bacteria 9 ko02000 M00306 ... 4 GO:0006950 response to stress 4 GO:0006259 DNA metabolic process 3 GO:0008150 biological_process 2 GO:0009058 biosynthetic ... PTS system, fructose-specific II-like component 8 ko03400 DNA repair and recombination proteins 8 ko03032 DNA replication ...
  http://link.g-language.org
*  The World Outside: A Changing Environment and How it Affects Us - Robert Sapolsky Rocks
Competence for natural transformation - capacity to take up naked DNA into its own genome. The bacteria can make a quantum leap ... This horizontal gene transfer generally requires that similar sequences of DNA surround the DNA to be added; this enables the ... When it doesn't take the DNA up altogether, it can degrade the source material to nucleotides and then take those up instead.. ... Humans are able to use the polymerase chain reaction along with an innocuous virus to introduce snippets of DNA into our own ...
  http://www.robertsapolskyrocks.com/the-world-outside-a-changing-environment-and-how-it-affects-us.html
*  Gary Schoolnik | Stanford Medicine Profiles
Transformation competence was found to require a type IV pilus assembly complex, a putative DNA binding protein, and three ... DNA hybridization studies of genomic DNA from the alpha- and beta-piliated variants of strain Epp63 indicated that the ... Colony hybridizations showed that most normal fecal E. coli do not contain hly and mrh DNA but much of the intervening DNA ... To determine the variability in human papillomavirus (HPV) DNA testing of the cervix in young women found positive for HPV DNA ...
  https://med.stanford.edu/profiles/gary-schoolnik
*  Transformation of Leuconostoc carnosum 4010 and evidence for natural competence of the organism - DTU Orbit
Optimized conditions resulted in transformation frequencies of up to 6.7 x 10(5) transformants per microgram of plasmid DNA. A ... Transformation of Leuconostoc carnosum 4010 and evidence for natural competence of the organism. Publication: Research - peer- ... Plasmid transformation in Leuconostoc carnosum 4010 was analyzed. A successful transformation protocol for L. carnosum was ... Interestingly, we discovered that DNA uptake was of a frequency of 3 x 10(-6) to 19 x 10(-6) transformants per CFU in the ...
  http://orbit.dtu.dk/en/publications/transformation-of-leuconostoc-carnosum-4010-and-evidence-for-natural-competence-of-the-organism
*  Difference between revisions of "User:Jen17" - OpenWetWare
Regulation of competence development Competence for genetic transformation is the ability of cells to take up naked DNA from ... Competence for genetic transformation is the ability of cells to take up naked DNA from the environment and incorporate this ... Competence for genetic transformation is the ability of cells to take up naked DNA from the environment and incorporate this ... DNA uptake during bacterial transformation. 2:241-9.. In addition to expressing the proteins required for DNA uptake and ...
  https://openwetware.org/wiki/?title=User:Jen17&diff=prev&oldid=109227
*  Biochemical Genetics and Molecular Biology: The Contributions of George Beadle and Edward Tatum | Genetics
... and the ability of the organisms to respond to the DNA (competence) was hard to control. Only a few laboratories had the ... 1944 Studies on the chemical nature of the substance inducing transformation of pneumococcal types: induction of transformation ... in providing selectable markers that made work on pneumococcal DNA transformation more generally accessible. ... species of DNA base composition and finally the illumination of the Watson-Crick structure for DNA (Watson and Crick 1953) were ...
  http://www.genetics.org/content/203/1/13
*  T. Tønjum's group - Microbiology - Laboratory Medicine - Divisions - Oslo University Hospital Research
Competence for natural transformation in Neisseria gonorrhoeae: components of DNA binding and uptake linked to type IV pilus ... The Inner Membrane Protein PilG Interacts with DNA and the Secretin PilQ in Transformation. PLoS One, 10 (8), e0134954. PubMed ... DprA from Neisseria meningitidis: properties and role in natural competence for transformation. Microbiology, 163 (7), 1016- ... New functional identity for the DNA uptake sequence in transformation and its presence in transcriptional terminators. J ...
  https://www.ous-research.no/home/tonjum/Group%20members/6798
*  Transformation efficiency - Wikipedia
10-100 pg of DNA may be used for transformation, more DNA may be necessary for low-efficiency transformation (generally ... This is based on the competence of the cells. It can be calculated by dividing the number of successful transformants by the ... Transformation efficiency can be measured in transformants or colony forming unit (cfu) per μg DNA used. A transformation ... Transformation efficiency is the efficiency by which cells can take up extracellular DNA and express genes encoded by it. ...
  https://en.wikipedia.org/wiki/Transformation_efficiency