*  Phylogeographic diversity and mosaicism of the Helicobacter pylori tfs integrative and conjugative elements | SpringerLink
Hofreuter D, Odenbreit S, Henke G, Haas R. Natural competence for DNA transformation in Helicobacter pylori: identification and ... pylori strains and is required for natural DNA transformation competence [33].. Components of the tfs4 ICE have also been found ... Both DNA transformation and conjugative processes are considered to contribute to the variability of gene content encoded by ... Transformation of the data to enable clustering on the basis of phylogeographic population highlights an apparent increase in ...
  https://link.springer.com/article/10.1186/s13100-018-0109-4
*  ASMscience | Type IV Secretion System
... it is not known whether DNA can be translocated into host cells during L. pneumophila infection. The popularity of employing ... Although the Dot/Icm T4SS transfers DNA by conjugation in vitro, ... Natural competence for DNA transformation in Helicobacter ... Natural transformation competence in Helicobacter pylori is mediated by the basic components of a type IV secretion system. Mol ... Although the Dot/Icm T4SS transfers DNA by conjugation in vitro, it is not known whether DNA can be translocated into host ...
  http://www.asmscience.org/content/book/10.1128/9781555815851.ch15
*  ASMscience | Genetic Exchange
Transformation is defined as the mechanism by which exogenous DNA is taken up by bacteria and the DNA becomes heritable. In a ... The use of conjugation as a mechanism for DNA delivery may have the advantage of bypassing restriction barriers that could ... Conjugation may provide an important method for performing genetic manipulations in strains resistant to transformation. ... inhibit genetic exchange by natural transformation. Several potential barriers exist to genetic exchange for H. pylori. ...
  http://www.asmscience.org/content/book/10.1128/9781555818005.chap28
*  Protocols and Video Articles Authored by Tarek Msadek (Translated to French)
Expression of a Cryptic Secondary Sigma Factor Gene Unveils Natural Competence for DNA Transformation in Staphylococcus Aureus ... is able to develop natural competence for transformation by DNA. We previously showed that a novel staphylococcal secondary ... which requires the expression of SigH-controlled competence genes. Additionally, using DNA from the N315 MRSA strain, we ... and used in DNA binding assays. CtsR from both species bound specifically to the ftsH promoter, generating a single protein-DNA ...
  https://www.jove.com/author/Tarek_Msadek?language=French
*  Protocols and Video Articles Authored by Kazuya Morikawa (Translated to Dutch)
Expression of a Cryptic Secondary Sigma Factor Gene Unveils Natural Competence for DNA Transformation in Staphylococcus Aureus ... is able to develop natural competence for transformation by DNA. We previously showed that a novel staphylococcal secondary ... DNA-topology assays using the cell extract showed that the extracts from the Deltafis and Topo I-/DNA gyrase-overexpressing ... Nevertheless, the main competence regulators and a series of orthologous genes that form the competence machinery are present ...
  https://www.jove.com/author/Kazuya_Morikawa?language=Dutch
*  Protocols and Video Articles Authored by Nguyen Thi Le Thuy (Translated to Korean)
Expression of a Cryptic Secondary Sigma Factor Gene Unveils Natural Competence for DNA Transformation in Staphylococcus Aureus ... is able to develop natural competence for transformation by DNA. We previously showed that a novel staphylococcal secondary ... which requires the expression of SigH-controlled competence genes. Additionally, using DNA from the N315 MRSA strain, we ... aureus cells producing active SigH become competent for transformation by plasmid or chromosomal DNA, ...
  https://www.jove.com/author/Nguyen_Thi+Le+Thuy?language=Korean
*  Frontiers | Modeling of the ComRS Signaling Pathway Reveals the Limiting Factors Controlling Competence in Streptococcus...
In conclusion, we propose that the level of ComR production is one important factor that could explain competence heterogeneity ... comR overexpression in both strains was shown to induce higher competence levels with deregulated kinetics patterns during ... concentration is the main limiting factor for the level of comX expression and controls the kinetics of spontaneous competence ... concentration is the main limiting factor for the level of comX expression and controls the kinetics of spontaneous competence ...
  https://www.frontiersin.org/articles/10.3389/fmicb.2015.01413/full
*  Staphylococcus aureus - Wikipedia
"Expression of a cryptic secondary sigma factor gene unveils natural competence for DNA transformation in Staphylococcus aureus ... Natural genetic transformation is a reproductive process involving DNA transfer from one bacterium to another through the ... Further studies suggested that the development of competence for natural genetic transformation may be substantially higher ... S. aureus was found to be capable of natural genetic transformation, but only at low frequency under the experimental ...
  https://en.wikipedia.org/wiki/Staphylococcus_aureus
*  Structure and mechanism of the PilF DNA transformation ATPase from Thermus thermophilus | Biochemical Journal
... twitching motility and DNA transformation. DNA transformation, also known as competence, is a process by which bacteria can ... TtPilF-DNA complex retained DNA (top panel, Figure 3C), compared with polyAT DNA alone (bottom panel, Figure 3C). The ATPase ... Structure and mechanism of the PilF DNA transformation ATPase from Thermus thermophilus. Richard F. Collins, Darin Hassan, ... thermophilus can take up DNA with high efficiency and has been shown to have a broad DNA specificity [3]. DNA translocation and ...
  http://www.biochemj.org/content/450/2/417
*  SAUSA300 RS11115 - AureoWiki
Cellular processesCellular processesDNA transformationcompetence protein ComEA helix-hairpin-helix repeat region (TIGR00426; ... Cellular processesCellular processesDNA transformationcomEA protein (TIGR01259; HMM-score: 40.9) ... Genetic information processingTranscriptionDNA-dependent RNA polymeraseDNA-directed RNA polymerase (TIGR00448; EC 2.7.7.6; HMM- ... no clan definedHTH_44; Helix-turn-helix DNA-binding domain of SPT6 (PF14641; HMM-score: 10.8) ...
  http://aureowiki.med.uni-greifswald.de/SAUSA300_RS11115
*  Transformation (genetics) - Wikipedia
suggested that competence for transformation probably evolved as a DNA damage response. Logarithmically growing bacteria differ ... Competence is specifically induced by DNA damaging conditions. For instance, transformation is induced in Streptococcus ... "Low-level pilin expression allows for substantial DNA transformation competence in Neisseria gonorrhoeae". Infection and ... transformation is increased by UV light, a DNA damaging agent. In Helicobacter pylori, ciprofloxacin, which interacts with DNA ...
  https://en.wikipedia.org/wiki/Transformation_(genetics)
*  SAUSA300 RS08435 - AureoWiki
Cellular processesCellular processesDNA transformationDNA internalization-related competence protein ComEC/Rec2 (TIGR00361; HMM ... DNA internalization-related competence protein ComEC/Rec2 ... no clan definedCompetence; Competence protein (PF03772; HMM- ...
  http://aureowiki.med.uni-greifswald.de/SAUSA300_RS08435
*  SACOL RS08170 - AureoWiki
Cellular processesCellular processesDNA transformationDNA internalization-related competence protein ComEC/Rec2 (TIGR00361; HMM ...
  http://aureowiki.med.uni-greifswald.de/SACOL_RS08170
*  NWMN 1449 - AureoWiki
Cellular processesCellular processesDNA transformationDNA internalization-related competence protein ComEC/Rec2 (TIGR00361; HMM ...
  http://aureowiki.med.uni-greifswald.de/NWMN_1449
*  Transporter Classification Database - Wikipedia
Na+-translocating Pyrophosphatase family 3.A.11 The Bacterial Competence-related DNA Transformation Transporter (DNA-T) Family ... 3.A.12 The Septal DNA Translocator (S-DNA-T) Family 3.A.13 The Filamentous Phage Exporter (FPhE) Family 3.A.14 The Fimbrilin/ ... Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family 3.A.8 The Mitochondrial Protein Translocase (MPT) Family ... Family 1.D.46 The DNA Nanopore (DnaNP) Family 1.D.47 The Pore-forming Synthetic Cyclic Peptide (PSCP) Family 1.D.48 The Pore- ...
  https://en.wikipedia.org/wiki/Transporter_Classification_Database
*  Bacterial natural transformation by highly fragmented and damaged DNA | PNAS
... closely related competence genes in Acinetobacter sp. strain BD413 and Thermus thermophilus HB27. Appl Environ Microbiol 67(7): ... E) Diagram of the ancient DNA experiment. Woolly mammoth DNA was used as donor DNA for natural transformation of the hisC::′ ... The data confirmed that transformation by short DNA was associated with DNA replication; in ,97% of transformants, the DNA ... Natural transformation experiments were performed as described in ref. 47 and SI Text, using as donor DNA chromosomal DNA, PCR ...
  http://www.pnas.org/content/110/49/19860
*  Archive | mSystems
In the cariogenic pathogen Streptococcus mutans, DNA transformation is regulated by the competence stimulating peptide XIP (Com ... Genetic competence provides bacteria with an opportunity to increase genetic diversity or acquire novel traits conferring a ... Transcriptional Profiling of the Oral Pathogen Streptococcus mutans in Response to Competence Signaling Peptide XIP ... The Baltic Sea Virome: Diversity and Transcriptional Activity of DNA and RNA Viruses ...
  http://msystems.asm.org/content/2/1
*  Neisseria gonorrhoeae - Wikipedia
"Competence for natural transformation in Neisseria gonorrhoeae: Components of DNA binding and uptake linked to type IV pilus ... The process in which recipient gonococci integrate DNA from neighboring gonococci into their genome is called transformation. ... have suggested that N. gonorrhoeae may replace DNA damaged in neutrophil phagosomes with DNA from neighboring gonococci. ... In addition to the ability to rearrange the genes it already has, it is also naturally competent to acquire new DNA, via its ...
  https://en.wikipedia.org/wiki/Neisseria_gonorrhoeae
*  The Streptococcus sanguinis Competence Regulon Is Not Required for Infective Endocarditis Virulence in a Rabbit Model
100-gene com regulon in addition to DNA uptake, including virulence. We investigated this possibility in S. sanguinis. Strains ... Genetic competence in S. sanguinis requires a quorum sensing system encoded by the early comCDE genes, as well as late genes ... Transformation Assays. Transformation was used in mutant generation and as a measure of competence using a modification of a ... S. sanguinis exhibits genetic competence [12], [13], the ability to acquire and incorporate exogenous DNA into its genome. This ...
  https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0026403
*  Review. Bacterial type IV secretion mechanisms | Philosophical Transactions of the Royal Society B: Biological Sciences
2006 Functional and topological characterization of novel components of the comB DNA transformation competence system in ... 2002 Natural transformation in Helicobacter pylori: DNA transport in an unexpected way. Trends Microbiol. 10, 159-162. doi: ... mediate DNA uptake from the environment leading to bacterial transformation [6]. Both dissemination and transformation ... Natural transformation of H. pylori is exceptional because it involves a T4SS ComB [23,24]. Recent evidence indicates that DNA ...
  http://rstb.royalsocietypublishing.org/content/367/1592/1073
*  ASMscience | Bacillus subtilis and Ot
... and competence for transformation by exogenous DNA (ComP-ComA and DegS-DegU) in Bacillus subtilis. These pairs are the focus of ... The implications of competence- linked induction of DNA repair genes and a possible mechanism for competence-linked induction ... coli DNA polymerase I and coliphage T7 DNA polymerase in the region that is believed to encode the DNA-binding domain and to ... ø29 DNA ends, suggesting that other proteins involved in the initiation of ø29 DNA replication (TP and/or DNA polymerase) ...
  http://www.asmscience.org/content/book/10.1128/9781555818388
*  ASMscience | Genetic Recombination
This chapter talks about competence and transformation in their bacterial senses (competence to take up DNA, genetic ... DNA exonucleases, enzymes that hydrolyze phosphodiester bonds in DNA from a free end, play important cellular roles in DNA ... The purpose of this DNA unwinding is to provide nascent, single-stranded DNA (ssDNA) for the processes of DNA repair, ... showed that DNA damage did not induce competence in either H. influenzae or B. subtilis. Proponents of the transformation ...
  http://www.asmscience.org/content/concept/Entity/ASM/Microbiology/Microbial_Genetics_and_Evolution/Microbial_Genetics/Genetic_Recombination
*  DNA Transport across the Outer and Inner Membranes of Naturally Transformable Vibrio cholerae Is Spatially but Not Temporally...
The physiological state of natural competence for transformation allows certain bacteria to take up free DNA from the ... To better understand these mechanisms, we investigated the competence-mediated DNA transport in the naturally transformable ... which suggested the competence protein ComEA plays a role in the DNA uptake process across the outer membrane of Gram-negative ... DNA Transport across the Outer and Inner Membranes of Naturally Transformable Vibrio cholerae Is Spatially but Not Temporally ...
  https://infoscience.epfl.ch/record/201514
*  Exogenous DNA - Wikipedia
The introduction of exogenous DNA into a cell is called transformation (transfection in animal cells). This can take place ... which is described as one of competence. Some bacteria are naturally competent, but usually only for a brief time at a certain ... In this method DNA is coated with lipid. Fusion of this lipid and the membrane lipid can occur, facilitating the entry of DNA. ... The use of exogenous DNA to transform cells has spawned the discipline of transgenesis: the use of recombinant DNA techniques ...
  https://en.wikipedia.org/wiki/Exogenous_DNA