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*  Microarray genomotyping of key experimental strains of Neisseria gonorrhoeae reveals gene complement diversity and five new...
DNA transformation and DNA repair. Mol Microbiol. 1998, 30: 697-710. 10.1046/j.1365-2958.1998.01089.x.View ArticlePubMedGoogle ... Natural competence for transformation and homologous recombination allow the generation of mosaic genes, such as the divergent ... DNA extraction, labelling, and microarray hybridization. Chromosomal DNA was extracted using the AquaPure Genomic DNA Isolation ... PCR amplification from chromosomal DNA was performed using BIO-X-ACT™ Long DNA Polymerase (BIOLINE) or Taq DNA polymerase ( ...
  https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-5-23
*  ASMscience | Role of Phase and Antige
Shuttle mutagenesis of Neisseria gonorrhoeae: pilin null mutations lower DNA transformation competence. J. Bacteriol. 172:40-46 ... Low-level pilin expression allows for substantial DNA transformation competence in Neisseria gonorrhoeae. Infect. Immun. 71: ... Transformation competence and type-4 pilus biogenesis in Neisseria gonorrhoeae-a review. Gene 192:125-134.. ... Role of pili and the phase-variable PilC protein in natural competence for transformation of Neisseria gonorrhoeae. Proc. Natl ...
  http://www.asmscience.org/content/book/10.1128/9781555817619.chap23
*  Genetic Manipulations with Plant Material | SpringerLink
The Mechanism of Competence for DNA Uptake and Transformation in Pneumococci. * The Mechanism of Competence for DNA Uptake and ... DNA-Hybridization Studies of the Fate of Bacterial DNA in Plants. * DNA-Hybridization Studies of the Fate of Bacterial DNA in ... Uptake of DNA and Bacteriophage into Pollen and Genetic Manipulation. * Uptake of DNA and Bacteriophage into Pollen and Genetic ... DNA Mediated Genetic Correction of Thiamineless Arabidopsis Thaliana * DNA Mediated Genetic Correction of Thiamineless ...
  https://link.springer.com/book/10.1007/978-1-4684-2763-9
*  Phylogeographic diversity and mosaicism of the Helicobacter pylori tfs integrative and conjugative elements | SpringerLink
Hofreuter D, Odenbreit S, Henke G, Haas R. Natural competence for DNA transformation in Helicobacter pylori: identification and ... pylori strains and is required for natural DNA transformation competence [33].. Components of the tfs4 ICE have also been found ... Both DNA transformation and conjugative processes are considered to contribute to the variability of gene content encoded by ... Transformation of the data to enable clustering on the basis of phylogeographic population highlights an apparent increase in ...
  https://link.springer.com/article/10.1186/s13100-018-0109-4
*  lecture 4 Flashcards by Molly Fowler | Brainscape
natural transformation competence-exchange of DNA 21 What is BabA? - blood group antigen-binding adhesion - facilitates ... large section of DNA that can be acquired by bacterial species -- stains may be Cag+ or Cag- -- contributes to pathogenicity ... Transformation into low-grade extra-nodal B cell lymphoma -- direct antigen stimulation by H. pylori -- mutations caused by ...
  https://www.brainscape.com/flashcards/lecture-4-2021119/packs/3676829
*  ASMscience | Type IV Secretion System
... it is not known whether DNA can be translocated into host cells during L. pneumophila infection. The popularity of employing ... Although the Dot/Icm T4SS transfers DNA by conjugation in vitro, ... Natural competence for DNA transformation in Helicobacter ... Natural transformation competence in Helicobacter pylori is mediated by the basic components of a type IV secretion system. Mol ... Although the Dot/Icm T4SS transfers DNA by conjugation in vitro, it is not known whether DNA can be translocated into host ...
  http://www.asmscience.org/content/book/10.1128/9781555815851.ch15
*  ASMscience | Genetic Exchange
Transformation is defined as the mechanism by which exogenous DNA is taken up by bacteria and the DNA becomes heritable. In a ... The use of conjugation as a mechanism for DNA delivery may have the advantage of bypassing restriction barriers that could ... Conjugation may provide an important method for performing genetic manipulations in strains resistant to transformation. ... inhibit genetic exchange by natural transformation. Several potential barriers exist to genetic exchange for H. pylori. ...
  http://www.asmscience.org/content/book/10.1128/9781555818005.chap28
*  No questions in Phenotype - lookformedical.com
DNA Transformation Competence (0) * Founder Effect (0) * Gene Frequency (0) * Gene Order (0) ...
  https://lookformedical.com/answers/en/phenomena-and-processes/genetic-phenomena/phenotype
*  No questions in DNA Repair - lookformedical.com
The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. ... DNA Transformation Competence (0) * Founder Effect (0) * Gene Frequency (0) * Gene Order (0) ...
  https://lookformedical.com/answers/en/phenomena-and-processes/genetic-phenomena/genetic-processes/dna-repair
*  No questions in Chromatin Assembly and Disassembly - lookformedical.com
... of that specific physical conformation of CHROMATIN determining the transcriptional accessibility or inaccessibility of the DNA ... DNA Transformation Competence (0) * Founder Effect (0) * Gene Frequency (0) * Gene Order (0) ...
  https://lookformedical.com/answers/en/phenomena-and-processes/genetic-phenomena/genetic-processes/gene-expression-regulation/chromatin-assembly-and-disassembly
*  С ?? ҩ ??.??Ϣ?? .11154501??11155000
11154894:Functional and Topological Characterization of Novel Components of the comB DNA Transformation Competence System in ... 11154833:Recruitment of Bacillus subtilis RecN to DNA Double-Strand Breaks in the Absence of DNA End Processing ... 11154877:DNA Sequence of a ColV Plasmid and Prevalence of Selected Plasmid-Encoded Virulence Genes among Avian Escherichia coli ... 11154860:Dissection of the Bacteriophage Mu Strong Gyrase Site(SGS): Significance of the SGS Right Arm in Mu Biology and DNA ...
  http://www.100md.com/index/other/byinfono/022/310.htm
*  The number of Neisseria meningitidis type IV pili determines host cell interaction | The EMBO Journal
... level pilin expression allows for substantial DNA transformation competence in Neisseria gonorrhoeae. Infect Immun 71: 6279- ... Transformation assay. Neisseria meningitidis grown on agar plates were resuspended in transformation medium (15 mg/ml proteose ... DNA exchange between different N. meningitidis strains occurs via the natural transformation properties mediated by type IV ... A type IV pilus mediates DNA binding during natural transformation in Streptococcus pneumoniae. PLoS Pathog 9: e1003473. ...
  http://emboj.embopress.org/content/33/16/1767
*  Protocols and Video Articles Authored by Tarek Msadek (Translated to French)
Expression of a Cryptic Secondary Sigma Factor Gene Unveils Natural Competence for DNA Transformation in Staphylococcus Aureus ... is able to develop natural competence for transformation by DNA. We previously showed that a novel staphylococcal secondary ... which requires the expression of SigH-controlled competence genes. Additionally, using DNA from the N315 MRSA strain, we ... and used in DNA binding assays. CtsR from both species bound specifically to the ftsH promoter, generating a single protein-DNA ...
  https://www.jove.com/author/Tarek_Msadek?language=French
*  Protocols and Video Articles Authored by Nguyen Thi Le Thuy (Translated to Korean)
Expression of a Cryptic Secondary Sigma Factor Gene Unveils Natural Competence for DNA Transformation in Staphylococcus Aureus ... is able to develop natural competence for transformation by DNA. We previously showed that a novel staphylococcal secondary ... which requires the expression of SigH-controlled competence genes. Additionally, using DNA from the N315 MRSA strain, we ... aureus cells producing active SigH become competent for transformation by plasmid or chromosomal DNA, ...
  https://www.jove.com/author/Nguyen_Thi+Le+Thuy?language=Korean
*  Protocols and Video Articles Authored by Kazuya Morikawa (Translated to Swedish)
Expression of a Cryptic Secondary Sigma Factor Gene Unveils Natural Competence for DNA Transformation in Staphylococcus Aureus ... is able to develop natural competence for transformation by DNA. We previously showed that a novel staphylococcal secondary ... DNA-topology assays using the cell extract showed that the extracts from the Deltafis and Topo I-/DNA gyrase-overexpressing ... Nevertheless, the main competence regulators and a series of orthologous genes that form the competence machinery are present ...
  https://www.jove.com/author/Kazuya_Morikawa?language=Swedish
*  Frontiers | Modeling of the ComRS Signaling Pathway Reveals the Limiting Factors Controlling Competence in Streptococcus...
In conclusion, we propose that the level of ComR production is one important factor that could explain competence heterogeneity ... comR overexpression in both strains was shown to induce higher competence levels with deregulated kinetics patterns during ... concentration is the main limiting factor for the level of comX expression and controls the kinetics of spontaneous competence ... concentration is the main limiting factor for the level of comX expression and controls the kinetics of spontaneous competence ...
  https://www.frontiersin.org/articles/10.3389/fmicb.2015.01413/full
*  Horizontal DNA Transfer Mechanisms of Bacteria as Weapons of Intragenomic Conflict. - Oxford Big Data Institute
Both constitutive and transient competence for transformation were found to provide an effective defence against parasitic MGEs ... we found multiple examples of MGEs inhibiting transformation through integrative disruption of genes encoding the competence ... and genetic transformation. Including the known bias of transformation toward the acquisition of shorter alleles into the model ... rates of transformation have also been observed in cells infected by MGEs that reduce the concentration of extracellular DNA ...
  https://www.bdi.ox.ac.uk/publications/609514
*  ASMscience | Eukaryotic-Like Proteins
Natural competence for DNA transformation by Le-gionella pneumophila and its association with expression of type IV pili. J. ... A) lpgl905,(B) lpgl488, (C) lpg0971, (D) lpg2644. DNA, genomic DNA control; RT +, reverse transcription PCR; RT-, control PCR ... A) lpgl905,(B) lpgl488, (C) lpg0971, (D) lpg2644. DNA, genomic DNA control; RT +, reverse transcription PCR; RT-, control PCR ...
  http://www.asmscience.org/content/book/10.1128/9781555815660.ch61
*  Staphylococcus aureus - Wikipedia
"Expression of a cryptic secondary sigma factor gene unveils natural competence for DNA transformation in Staphylococcus aureus ... Natural genetic transformation is a reproductive process involving DNA transfer from one bacterium to another through the ... Further studies suggested that the development of competence for natural genetic transformation may be substantially higher ... S. aureus was found to be capable of natural genetic transformation, but only at low frequency under the experimental ...
  https://en.wikipedia.org/wiki/Staphylococcus_aureus
*  Structure and mechanism of the PilF DNA transformation ATPase from Thermus thermophilus | Biochemical Journal
... twitching motility and DNA transformation. DNA transformation, also known as competence, is a process by which bacteria can ... TtPilF-DNA complex retained DNA (top panel, Figure 3C), compared with polyAT DNA alone (bottom panel, Figure 3C). The ATPase ... Structure and mechanism of the PilF DNA transformation ATPase from Thermus thermophilus. Richard F. Collins, Darin Hassan, ... thermophilus can take up DNA with high efficiency and has been shown to have a broad DNA specificity [3]. DNA translocation and ...
  http://www.biochemj.org/content/450/2/417
*  SAUSA300 RS11115 - AureoWiki
Cellular processesCellular processesDNA transformationcompetence protein ComEA helix-hairpin-helix repeat region (TIGR00426; ... Cellular processesCellular processesDNA transformationcomEA protein (TIGR01259; HMM-score: 40.9) ... Genetic information processingTranscriptionDNA-dependent RNA polymeraseDNA-directed RNA polymerase (TIGR00448; EC 2.7.7.6; HMM- ... no clan definedHTH_44; Helix-turn-helix DNA-binding domain of SPT6 (PF14641; HMM-score: 10.8) ...
  http://aureowiki.med.uni-greifswald.de/SAUSA300_RS11115
*  Pathogenesis-related Proteins From Helicobacter pylori: Structural and Functional Studies - Padua@Research
Functional and topological characterization of novel components of the comB DNA transformation competence system in ... 2003b) Extensive repetitive DNA facilitates prokaryotic genome plasticity. Proc Natl Acad Sci U S A. 100 (23):13579-84. Cerca ... 2003a) Plasticity of repetitive DNA sequences within a bacterial (Type IV) secretion system component. J Exp Med. 198 (9):1349- ... Fischer W, Hofreuter D, Haas R. (2001) Natural Transformation, Recombination, and Repair. In: Mobley HLT, Mendz GL, Hazell SL, ...
  http://paduaresearch.cab.unipd.it/4325/
*  Expression capable library for studies of Neisseria gonorrhoeae, version 1.0 | BMC Microbiology | Full Text
In addition, the DNA arrays produced will allow us to generate gene expression profiles of gonococci grown in a wide variety of ... PCR amplicons of each ORF used in the cloning reactions were spotted onto glass slides to produce DNA microarrays representing ... pilin null mutations lower DNA transformation competence. J Bacteriol. 1990, 172: 40-46.PubMed CentralPubMedGoogle Scholar. ... DNA microarrays were generated by spotting the insert DNA onto glass slides. Preliminary experiments with the clone set and DNA ...
  https://0-bmcmicrobiol-biomedcentral-com.brum.beds.ac.uk/articles/10.1186/1471-2180-5-50
*  Transformation (genetics) - Wikipedia
suggested that competence for transformation probably evolved as a DNA damage response. Logarithmically growing bacteria differ ... Competence is specifically induced by DNA damaging conditions. For instance, transformation is induced in Streptococcus ... "Low-level pilin expression allows for substantial DNA transformation competence in Neisseria gonorrhoeae". Infection and ... transformation is increased by UV light, a DNA damaging agent. In Helicobacter pylori, ciprofloxacin, which interacts with DNA ...
  https://en.wikipedia.org/wiki/Transformation_(genetics)