Crystal structure of the C-terminal domain of DNA topoisomerase IV ... Bacteria possess two closely related yet functionally distinct essential type IIA topoisomerases (Topos). DNA gyrase supports ... Structure of the topoisomerase IV C-terminal domain: a broken beta-propeller implies a role as geometry facilitator in ... Crystal structure of the C-terminal domain of DNA topoisomerase IV. *PDB DOI: https://doi.org/10.2210/pdb1WP5/pdb ...

Watt PM, Hickson ID (November 1994). "Structure and function of type II DNA topoisomerases". The Biochemical Journal. 303 (Pt 3 ... DNA topoisomerase IIα is a human enzyme encoded by the TOP2A gene. Topoisomerase IIα relieves topological DNA stress during ... Topoisomerase II TOP2B - Topoisomerase II beta Gene duplication Ataxia-telangiectasia TOPBP1 GRCh38: Ensembl release 89: ... "Entrez Gene: TOP2A topoisomerase (DNA) II alpha 170kDa". Emanuelli A, Borroni AP, Apel-Sarid L, Shah PA, Ayyathan DM, Koganti P ...

DNA Topoisomerases. EcoSal Plus. 2015:6 3. Watt PM, Hickson ID. Structure and function of type II DNA topoisomerases. The ... DNA topoisomerases can be classified as type I and type II enzymes [3, 6]. TOP2A is one of the isoenzymes which can mediate the ... catalytic activity of type II topoisomerases [6].. TOP2A (DNA topoisomerase II alpha) gene, mapped to chromosome 17q12-q21, ... 5. Bakshi RP, Galande S, Muniyappa K. Functional and regulatory characteristics of eukaryotic type II DNA topoisomerase. ...

Consequences for type II topoisomerase function",. abstract = "Type II topoisomerases resolve problematic DNA topologies such ... Electrostatics of DNA-DNA juxtapositions: Consequences for type II topoisomerase function. Journal of Physics Condensed Matter ... Electrostatics of DNA-DNA juxtapositions: Consequences for type II topoisomerase function. Graham L. Randall, B. Montgomery ... Electrostatics of DNA-DNA juxtapositions: Consequences for type II topoisomerase function. / Randall, Graham L.; Pettitt, B. ...

... the behavior of four nuclear matrix proteins during the various stages of apoptosis in the HL-60 cell line exposed to the DNA ... topoisomerase I inhibitor, camptothecin. We have examined the following antigens by immunocytochemical techniques: (i) the 180- ... DNA Topoisomerases, Type II / immunology * DNA Topoisomerases, Type II / metabolism * Fluorescent Antibody Technique, Indirect ... the 180-kDa nucleolar isoform of DNA topoisomerase II; (ii) a 126-kDa polypeptide of nuclear bodies; (iii) a 125-kDa protein; ...

Unfortunately, most drugs that target DNA topoisomerase II do not accumulate in wild type yeast cells to high enough. levels to ... We have found that vectors that overexpress topoisomerase II, or vectors that express a topoisomerase II mutant that is ... Novel DNA repair pathways required for cell survival following exposure to topoisomerase II targeting agents. Meiqin Liu; ... We are using this deletion set to identify all yeast genes that confer sensitivity to drugs targeting DNA topoisomerase II. ...

It has been proposed that both type IA and type II enzymes change conformation dramatically during the reaction cycle in order ... In order to change the topology of DNA, topoisomerases pass one or two DNA strands through transient single or double strand ... In order to change the topology of DNA, topoisomerases pass one or two DNA strands through transient single or double strand ... DNA topoisomerases are the enzymes responsible for maintaining the topological states of DNA. ...

IPR013824 DNA topoisomerase, type IA, central region, subdomain 1. IPR013825 DNA topoisomerase, type IA, central region, ... Cloning of cDNA encoding a novel mouse DNA topoisomerase III (Topo IIIbeta) possessing negatively supercoiled DNA relaxing ... IPR023405 DNA topoisomerase, type IA, core domain. IPR003602 DNA topoisomerase, type IA, DNA-binding domain ... IPR013826 DNA topoisomerase, type IA, central region, subdomain 3. ...

Our group has identified DNA topoisomerase IIα (Topo IIα) as one of the important mitotic proteins for SUMOylation. SUMOylated ... Mitotic SUMOylation: Unraveling the role of DNA Topoisomerase IIα SUMOylation and PIASy SUMO E3 ligase in mitosis. ... We have determined that Topo IIα CTD SUMOylation behaves like a signal transducer to induce a mitotic delay when Topo IIα is ... Further, we have shown that disruption of Topo II strand passage reaction (SPR) results in increased Topo IIα SUMOylation and ...

Topoisomerases are divided into two main types. Type I Topoisomerases act on one strand of double-stranded DNA, and they are ... Type II topoisomerases, on the other hand, are ATP-dependent and act on supercoiled DNA where the DNA is tangled around itself ... Type II topoisomerases are ATP-dependent enzymes that cut both strands of a DNA double helix. They are divided into two ... Type II topoisomerase reseals the broken ends of the DNA strands and finally detaches from the DNA- leaving behind an untangled ...

PDB Description: Topoisomerase VI-B, ADP-bound dimer form. PDB Compounds: (A:) Type II DNA topoisomerase VI subunit B. SCOPe ... Protein Topoisomerase VI-B subunit [82577] (1 species). contains an H2TH domain inserted in front of this domain and after the ... d1z5aa2 d.14.1.3 (A:307-469) Topoisomerase VI-B subunit {Sulfolobus shibatae [TaxId: 2286]} ... Family d.14.1.3: DNA gyrase/MutL, second domain [54224] (6 proteins). ...

The involvement of viral DNA-binding proteins in the regulation of virulence genes, transcription, DNA replication, and repair ... pA104R is a highly conserved HU/IHF-like DNA-packaging protein identified in the ASFV nucleoid that appears to be profoundly ... and a type II topoisomerase (P1192R), as well as the distinctive HU/IHF-like prokaryotic DNA-packaging protein (A104R). ... 3. The DNA Binding Protein pA104R 3.1. Structure of pA104R. ASFV ORF A104R (5-AR) predicts a type II DNA-binding protein of 104 ...

Coumermycin A1 efficacy was determined by the transcription of DNA gyrase, a type II DNA topoisomerase using reverse ... Objectives: Enrofloxacin, a fluoroquinolone antibiotic, is an inhibitor of prokaryotic topoisomerase II with antibacterial and ... Two separate groups (5 rabbits/each) treated topically (poured on at the base of the neck) with fipronil 5%, 1 vial/ 10 ... The two ruminant parasites, Paramphistomum cervi and Carmyerius gregarius, were collected from fresh-slaughtered native cattle ...

... these two domains are also found in the type II topoisomerase (DNA gyrase A) and in the alpha subunit of topoisomerase IV. ... from e.10.1.1 DNA topoisomerase I, 67K N-terminal domain: *Species Escherichia coli [TaxId:562] from e.10.1.1 DNA topoisomerase ... Fold e.10: Prokaryotic type I DNA topoisomerase [56711] (1 superfamily). 4 domains: (1) Toprim alpha/beta; (2&4) "winged helix ... Superfamily e.10.1: Prokaryotic type I DNA topoisomerase [56712] (2 families) duplication: the protein chain passing through ...

DNA binding - DNA replication - DNA topoisomerase (ATP-hydrolyzing) activity - DNA topoisomerase type I activity - DNA ... that OTIs can be used to direct the sites of etoposide-induced DNA cleavage mediated by topoisomerase IIα and topoisomerase IIβ ... DNA topoisomerases, especially type IIA topoisomerases, are proved therapeutic targets of anticancer and antibacterial drugs. ... Topoisomerase IIα expression correlated with cavernous sinus invasion.. CONCLUSIONS: The topoisomerase IIα expression ...

Which are released by DNA gyrases and topoisomerases *15. Termination  Two types rho dependent and rho independent.  ... Enzyme involved  DNA dependent RNA polymerase. The enzyme attaches itself at a specific on the DNA, the promoter site on the ... RNA editing  Central dogma is DNA  RNA  Protein. So change in DNA will be reflected into RNA and into protein, but sometimes ... Synthesis of RNA from DNA template  The primary transcript  DNA dependent RNA polymerase  Steps of RNA synthesis  RNA ...

... patients received epirubicin at clinically relevant doses after dose-escalation.Results of the topoisomerase activity will be ... regimen in patients with upper gastrointestinalmalignancies and breast cancer will be investigated as partof phase II studies, ... administered IV at a fixeddose of 250 mg/m2 on day 1 in combination with capecitabine at a fixeddose of 1,500 mg/m2 for days 2 ... Eukaryotic DNA topoisomerases: Two forms of type I DNA topoisomerases from HeLa cell nuclei. Proc Natl Acad Sci USA 78:3487- ...

... topoisomerases I, III and V) break single-strand DNA, and type II enzymes ( EC 5.99.1.3 ; topoisomerases II, IV and VI) break ... DNA Topoisomerase I (eukaryota), DNA topoisomerase V, Vaccina virus topoisomerase, Variola virus topoisomerase, Shope fibroma ... The type IB family of DNA topoisomerases includes eukaryotic nuclear topoisomerase I, topoisomerases of poxviruses, and ... Human DNA Topoisomerase I (70 Kda) In Complex With The Indenoisoquinoline MJ-II-38 and Covalent Complex With A 22 Base Pair DNA ...

DNA Topoisomerase VI: a type II DNA topoisomerase present in Archaea and Eukarya. Poster by Cyril Buhler , Joyce H.G. Lebbink ... An atypical type II DNA topoisomerase from Archaea with implication for meiotic recombination. Nature (386, 414-417, 1997). A ... The Eighth Conference on DNA Topoisomerases in Therapy . Intrinsic ATPase Activity of the B Subunit of DNA Topoisomerase VI ... October 21-24 , 1996, New York City:The Seventh Conference On Dna Topoisomerases In Therapy. Posters by A. Bergerat and D. ...

Type II DNA Topoisomerase 13% * Thin Layer Chromatography 12% * Protein Subunits 11% ... 深入研究「Identification of the breakage-reunion subunit of T4 DNA topoisomerase」主題。共同形成了獨特的指紋。 ...

N2 - Type-II DNA topoisomerases resolve DNA entanglements such as supercoils, knots and catenanes by passing one segment of DNA ... AB - Type-II DNA topoisomerases resolve DNA entanglements such as supercoils, knots and catenanes by passing one segment of DNA ... Type-II DNA topoisomerases resolve DNA entanglements such as supercoils, knots and catenanes by passing one segment of DNA ... "Type-II DNA topoisomerases resolve DNA entanglements such as supercoils, knots and catenanes by passing one segment of DNA ...

... both of which are type II topoisomerases), enzymes required for DNA replication, transcription, repair and recombination.. ... Fluoroquinolone resistance can arise through mutations in defined regions of DNA gyrase or topoisomerase IV, termed the ... Levofloxacin tablets should be administered at least two hours before or two hours after antacids containing magnesium, ... These agents should be taken at least two hours before or two hours after oral levofloxacin administration. ...

DNA gyrase) and topoisomerase IV (both Type II topoisomerases), which are required for bacterial DNA replication, transcription ... The tablets contain a combination of two types of ciprofloxacin drug substance, ciprofloxacin hydrochloride and ciprofloxacin ... The bactericidal action of ciprofloxacin results from inhibition of topoisomerase II ( ... Rat Hepatocyte DNA Repair Assay (Positive) Thus, 2 of the 8 tests were positive, but results of the following 3 in vivo test ...

Brown PO, Cozzarelli NR "Catenation and knotting of duplex DNA by type 1 topoisomerases: a mechanistic parallel with type 2 ... of two viral DNA ends in a bimolecular disintegration reaction mediated by multimers of human immunodeficiency virus type 1 or ... Tsuchihashi Z, Brown PO "DNA strand exchange and selective DNA annealing promoted by the human immunodeficiency virus type 1 ... Brown PO, Peebles CL, Cozzarelli NR "A topoisomerase from Escherichia coli related to DNA gyrase." Proc Natl Acad Sci U S A ...

Human DNA topoisomerase II and bacterial DNA topoisomerase IV decatenation assays. Request a detailed protocol These ... Ng were tested on a gonococcal typing (GC) agar base with 1% defined growth supplement and Nm were tested on Mueller-Hinton ... Decatenation assays using purified human DNA topoisomerase II (ProFoldin, Hudson, MA), Ec DNA topoisomerase IV (ProFoldin, ... PTC-847 targets DNA synthesis, but not DNA topoisomerases. Our previous studies (Gerasyuto et al., 2018) established that a ...

These antibiotics act on the type II topoisomerases, type IV topoisomerases and DNA gyrase, which inhibits their function and ... The DNA topoisomerase is essential for normal physiological functions of the bacteria like DNA replication, recombination and ... The DNA gyrase and topoisomerase IV create a break in the bacterial chromosome, which leads to the fragmentation of the ... The DNA strands break, and this break will reduce the cells ability to repair the damaged DNA, which leads to cell death. ...

"DNA supercoiling inhibits DNA knotting". April 15, 2008, "Type II Topoisomerase Models". October 23, 2007, "Resolution of DNA ... "DNA Unknotting by Human Topoisomerase IIα". *International Summer School on "DNA and Chromosomes: Physical and Biological ... "Unknotting by Type II Topoisomerases". *Celebration of the Career of Clay C. Ross, The University of the South, Sewanee, TN, ... "Topology of Type II Topoisomerases". *"Mathematics and Molecular Biology VII: Modeling Across the Scales- Atoms to Organisms," ...

Two type I topoisomerases maintain DNA topology in human mitochondria. Title: Two type I topoisomerases maintain DNA topology ... domain found in members of the type IA family of DNA topoisomerases (Topo IA) similar to topoisomerase III. Type IA DNA ... DNA topoisomerase 3-alpha. Names. topo III-alpha. topoisomerase (DNA) III alpha. zinc finger, GRF-type containing 7. NP_ ... TOP1Ac; DNA Topoisomerase, subtype IA; DNA-binding, ATP-binding and catalytic domain of bacterial DNA topoisomerases I and III ...

... did not induce significant DNA/topoisomerase I complex formation and did not inhibit RNA synthesis. Neither short-term nor long ... Transfer of a mutant p53 gene enhanced topotecan sensitivity in wild-type p53 LN-229 but not mutant p53 LN-18 cells. CD95 ... Topotecan is a novel topoisomerase I inhibitor that may have a role in the adjuvant chemotherapy of several solid tumors, ... induced DNA fragmentation, did not induce major cell cycle changes, failed to consistently alter BCL-2 or BAX protein levels ...