WikiGenes - DNA Shuffling
Associations of DNA Shuffling with chemical compounds. *Seven rounds of DNA shuffling and colony screening on chromogenic ... Disease relevance of DNA Shuffling. *Using the process of DNA shuffling, we randomly recombined the bphA1 genes of Pseudomonas ... Molecular evolution of an arsenate detoxification pathway by DNA shuffling .. *After three rounds of DNA shuffling and ... and rabbit by DNA shuffling and screening .. *To select for chimeric Pgp with an altered resistance profile, DNA shuffling ...https://www.wikigenes.org/e/mesh/e/20923.html
DNA shuffling - Wikipedia
... hence the term DNA shuffling. Restriction enzymes that cut in similar places are used to digest members of the gene family DNA ... DNA shuffling is a way to rapidly propagate beneficial mutations in a directed evolution experiment. It is used to rapidly ... Cohen, J. (13 July 2001). "How DNA Shuffling Works". Science. 293 (5528): 237. doi:10.1126/science.293.5528.237. Bacher, Jamie ... 2002). "Anticipatory evolution and DNA shuffling" (PDF). Genome Biology. 3 (8). ...https://en.wikipedia.org/wiki/DNA_shuffling
Stop GM Soya 356043!
DNA shuffling inherently hazardous Soybean 356043 involves a novel, untried technique called DNA- or gene-shuffling to create a ... As DNA shuffling can produce billions of recombinants in a matter of hours, it is unlikely that most of the recombinants will ... Death by DNA shuffling. Science in Society 18, 9, 2003.. *Pioneer Hi-Bred International Inc. Pioneer breaks new ground for ... The gene gat46014 enhanced using DNA shuffling, isolated from the bacterium Bacillus licheniformis, codes for an enzyme that ...http://www.i-sis.org.uk/stopGMsoya356043.php
Cytochrome P450 Protocols | SpringerLink
DNA Shuffling of Cytochrome P450 Enzymes James B. Y. H. Behrendorff, Wayne A. Johnston, Elizabeth M. J. Gillam ...https://link.springer.com/book/10.1007/978-1-62703-321-3
Antievolution.org - Antievolution.org Discussion Board -Topic::Co-option/change of function
DNA Shuffling It is possible that fewer than nine amino acid changes are required to interconvert melamine deaminase and ... In this context, DNA shuffling was conducted and the clonal variants were screened against a chemical library of substrates ... Here is a case of the origin of an autotransporter (AT) gene, lav by domain shuffling; I quote just a bit, the whole rather ... 10) Life Before DNA: The Origin and Evolution of Early Archean Cells. 11) The Prebiotic Evolution of Complex Molecules: A ...http://www.antievolution.org/cgi-bin/ikonboard/ikonboard.cgi?s=5b4d540d2f3a1737
Antievolution.org - Antievolution.org Discussion Board -Topic::Evolution of multiple-parts-required pathways
DNA Shuffling It is possible that fewer than nine amino acid changes are required to interconvert melamine deaminase and ... In this context, DNA shuffling was conducted and the clonal variants were screened against a chemical library of substrates ... was shown to hydrolyze ametryn, but it was not tested with atraton or other methoxy-functionalized herbicides (50). DNA from ... It is also of potential evolutionary significance that shuffled mutants were obtained with 80-fold enhanced activities with ...http://www.antievolution.org/cgi-bin/ikonboard/ikonboard.cgi?s=5a37524deb85066d
Advanced Gene Therapeutics
DNA Shuffling. *Directed Design. Related Conference of Advanced Gene Therapeutics. Dec 03-04, 2018 ...https://www.omicsonline.org/conferences-list/advanced-gene-therapeutics
CH391L/S13/DirectedProteinEvolution - OpenWetWare
DNA shuffling Screening techniques. List of high-throughput assays for protein function ...https://openwetware.org/wiki/?title=CH391L/S13/DirectedProteinEvolution&curid=130741&oldid=677266
Directed evolution of ampicillin-resistant activity from a functionally unrelated DNA fragment: A laboratory model of molecular...
DNA shuffling was done as described (1). Fragments of 100 to 300 bp were used to reassemble the 1.2-kb DNA. The rate of ... The activity of this clone was enhanced by 50 rounds of directed evolution by using DNA shuffling. Analysis of the evolved DNA ... The sequence of the original "preevolved" DNA, AR0, is aligned with that of AR50-3. PCR primers used for DNA shuffling anneal ... The 1.2-kb fragment was amplified from the plasmid mix by using PCR, and a subsequent round of DNA shuffling was done to ...http://www.pnas.org/content/98/3/903
Predicting Evolutionary Potential: In Vitro Evolution Accurately Reproduces Natural Evolution of the TEM β-Lactamase | Genetics
2001 DNA shuffling method for generating highly recombined genes and evolved enzymes. Nat. Biotechnol. 19: 354-359. ... 1996 Improved green fluorescent protein by molecular evolution using DNA shuffling. Nat. Biotechnol. 14: 315-319. ... 1994 Rapid evolution of a protein in vitro by DNA shuffling. Nature 370: 389-390. ... 1986 Some probabilistic and statistical problems on the analysis of DNA sequences. Lect. Math. Life Sci. 17: 57-86. ...http://www.genetics.org/content/160/3/823
High-resolution crystal structure of an avidin-related protein: insight into high-affinity biotin binding and protein stability...
A Novel Chimeric Avidin with Increased Thermal Stability Using DNA Shuffling. *Barbara Taskinen, Tomi T. Airenne, +4 authors ...https://www.semanticscholar.org/paper/High-resolution-crystal-structure-of-an-protein%3A-Eisenberg-Domovich-Hyt%C3%B6nen/371ea237bba82aa5bfd3b6b6789908d9cfba9321
US5958751A - α-galactosidase - Google Patents
DNA shuffling to produce herbicide selective crops US6500639B2 (en) 1998-10-07. 2002-12-31. Maxygen, Inc.. DNA shuffling to ... Optimization of pest resistance genes using DNA shuffling US6500639B2 (en) 1998-10-07. 2002-12-31. Maxygen, Inc.. DNA shuffling ... Optimization of pest resistance genes using DNA shuffling US6605430B1 (en) 1998-08-12. 2003-08-12. Maxygen, Inc.. DNA shuffling ... which DNA includes cDNA, genomic DNA, and synthetic DNA. The DNA may be double-stranded or single-stranded, and if single ...https://patents.google.com/patent/US5958751A/en
Environmental changes bridge evolutionary valleys | Science Advances
Rapid evolution of a protein in vitro by DNA shuffling. Nature 370, 389-391 (1994).. ... and plasmid DNA was isolated. Amplicons were generated directly from each plasmid DNA prep. During PCR, 3-bp barcodes ... Plasmid DNA was isolated from the pelleted cells. Amplicons were prepared for deep sequencing via PCR from each plasmid prep ... Thus, by deep sequencing the plasmid DNA to determine the frequency of each allele on each plate, the fitness for each mutant ...http://advances.sciencemag.org/content/2/1/e1500921.full
IJMS | Free Full-Text | Computational Protein Engineering: Bridging the Gap between Rational Design and Laboratory Evolution |...
Stemmer, W.P. Rapid evolution of a protein in vitro by DNA shuffling. Nature 1994, 370, 389-391. [Google Scholar] ... and DNA shuffling  have essentially revolutionized biocatalysis , as they allow enzyme properties to be altered through ... Xiang, Y.; Oelschlaeger, P.; Florian, J.; Goodman, M.F.; Warshel, A. Simulating the effect of DNA polymerase mutations on ... catalysts as biomimics of RNA and DNA phosphoryl transfer enzymes: Changing the medium from water to aclohol provides enzyme- ...http://www.mdpi.com/1422-0067/13/10/12428/htm
Algorithms for Joint Optimization of Stability and Diversity in Planning Combinatorial Libraries of Chimeric Proteins |...
Stemmer, W.: Rapid evolution of a protein in vitro by DNA shuffling. Nature 370(6488), 389-391 (1994)CrossRefGoogle Scholar ... Coco, W.: RACHITT: Gene family shuffling by random chimeragenesis on transient templates. Methods Mol. Biol. 231, 111-127 (2003 ...https://link.springer.com/chapter/10.1007%2F978-3-540-78839-3_26
Chemtrail/Contrail Mystery Spraying Solved? Is This What's Going On?
DNA shuffling. DNA shuffling is a revolutionary method for directed evolution. Maxygen uses a preferred format, called family ... Improved Vaccines by DNA Shuffling of Pathogen Antigens U.S. OSIRIS Therapeutics, Inc. Baltimore, MD. Sequential Auto ... Maxygen's expertise in DNA shuffling and library screening technologies.. Pathogenic spore-forming bacteria like Bacillus ... Activation of Innate Immunity by CpG DNA for Broad Spectrum Protection Against Pathogens U.S. University of Texas-Southwestern ...http://netowne.com/environmental/contrails/sightings/mystery.htm
Patent US7074590 - Chimeric promoters - Google Patents
"Rapid evolution of a protein in vitro by DNA shuffling" Nature 370:389-391; and Stemmer (1994) "DNA shuffling by random ... DNA shuffling is also sometimes referred to as molecular breeding directed molecular evolution (i.e., shuffling plus screening ... Method for constructing a library using dna shuffling. WO1998041623A1. 18 Mar 1998. 24 Sep 1998. Novo Nordisk A/S. Shuffling of ... Cells were transfected with a plasmid DNA comprising a shuffled promoter nucleic acid sequence, a plasmid DNA comprising a wild ...http://www.google.co.uk/patents/US7074590
2002 Miami Nature Biotechnology Winter Symposium; Advances in Gene Technology: The Genome and Beyond - Structural Biology for...
Molecular Breeding of Genes, Pathways and Genomes by Dna Shuffling, WIllem P.C. Stemmer Short Report (Pages 130-131), Volume 2 ... Some Aspects of Dna-Etbr Interaction Peculiarities, Poghos O. Vardevanyan, Ara P. Antonyan, Lia A. Minasbekyan, and Armen T. ... HRAD30A, a Gene Encoding a Class Y Low-Fidelity Dna Polymerase Has a Low Complexity Region in Intron I Associated with Tissue- ...https://www.hindawi.com/journals/tswj/si/686292/
Evidence of evolution - New World Encyclopedia
DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution. Proc Natl Acad Sci U S A ... Comparison of the DNA sequences allows organisms to be grouped by sequence similarity, and the resulting phylogenetic trees are ... The chirality of DNA, RNA, and amino acids is conserved across all known life. As there is no functional advantage to right- or ... Other overarching similarities between all lineages of extant organisms, such as DNA, RNA, amino acids, and the lipid bilayer, ...http://www.newworldencyclopedia.org/entry/Evidence_of_evolution
Bad Mom, Good Mom: December 2008
Every shuffle of DNA segments also carries the danger of producing an antibody that recognizes the body's own tissues. To avoid ... This phenomenal flexibility comes courtesy of a remarkable DNA shuffling system called V(D)J recombination. Just as riffling a ... shuffling specialized DNA segments in developing immune cells can produce a different antibody structure nearly every time. ... "All of us have developing lymphocytes in our bone marrow shuffling these antibody genes around continuously, from the time we ...http://badmomgoodmom.blogspot.com/2008/12/
US7323299B2 - Methods for in vivo diversification of single genes - Google Patents
The invention takes advantage of the induction of G:C to A:T transition mutations specific to duplicated DNA sequences during ... Crameri, A., Bermudez, E., Raillard, S., & Stemmer, W. P. C. (1998). DNA shuffling of a family of genes from diverse species ... Centromeric DNA from Saccharomyces cerevisiae. Nacry et al. 1998. Major chromosomal rearrangements induced by T-DNA ... Meiotic silencing by unpaired DNA: properties, regulation and suppression. Gordenin et al. 1993. Inverted DNA repeats: a source ...https://patents.google.com/patent/US7323299
Keystone Symposia | Scientific Conferences on Biomedical and Life Science Topics
Development of a Novel Vaccines using DNA Shuffling/Screening Strategies and Bacillus Spore Display ... Progress Towards Understanding the Properties of DNA Heather L. Davis, Pfizer Inc., Canada Use of CpG TLR9 Agonist as a Vaccine ... Enhancing DNA Vaccine Potency by Combining a Strategy to Prolong Dendritic Cell Life with Intracellular Targeting Strategies. ... DNA Vaccination Against Anthrax - An Update. Manisha Gupta, Centers for Disease Control, USA Perforin is Required for ...https://www.keystonesymposia.org/index.cfm?e=web.Meeting.Program&meetingid=706
Pennisi (1998): Molecular Evolution
If this is true, then they make possible DNA shuffling that can place genes in new regulatory contexts and, possibly, new roles ... They activate different repair enzymes and promote DNA shuffling, for example. Not long ago, these ideas would have been ... In test tube studies of DNA repair enzymes, Kunkel finds that the error rate for the repair of mistakes made during DNA ... What's more, some sequences are much more prone to error when the DNA is copied than others. In particular, DNA consisting of ...http://cogweb.ucla.edu/Abstracts/Pennisi_98.html
Multimeric oxidoreductases - Danisco US Inc.
"Rapid evolution of a protein in vitro by DNA shuffling," (1994) Nature 370:389-391. Stemmer W.P.C., "DNA shuffling by randon ... In vitro recombination may be accomplished by various well known means such as by DNA shuffling and variations of this ... Biotechnol 58: 1-14.). DNA shuffling relies on homologous recombination during PCR reassembly of gene fragments from multiple ... The expression vector may be incorporated into a plasmid, chromosome, mitochondrial DNA, plastid DNA, virus or nucleic acid ...http://www.freepatentsonline.com/8975048.html
Directed evolution - Wikipedia
Crameri, A; Raillard, SA; Bermudez, E; Stemmer, WP (15 January 1998). "DNA shuffling of a family of genes from diverse species ... Stemmer, WP (4 August 1994). "Rapid evolution of a protein in vitro by DNA shuffling". Nature. 370 (6488): 389-91. doi:10.1038/ ... Höss, M; Jaruga, P; Zastawny, TH; Dizdaroglu, M; Pääbo, S (1 April 1996). "DNA damage and DNA sequence retrieval from ancient ... Gene recombination can be mimicked by DNA shuffling of several sequences (usually of more than 70% homology) to jump into ...https://en.wikipedia.org/wiki/Directed_evolution