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*  How DNA Shuffling Works | Science
... that's used to amplify small amounts of DNA. But scientists at one biotech that is using the technique to produce vaccines have ... given it a special tweak to produce what they call "DNA shuffling." ...
  http://science.sciencemag.org/content/293/5528/237
*  WikiGenes - DNA Shuffling
Associations of DNA Shuffling with chemical compounds. *Seven rounds of DNA shuffling and colony screening on chromogenic ... Disease relevance of DNA Shuffling. *Using the process of DNA shuffling, we randomly recombined the bphA1 genes of Pseudomonas ... Molecular evolution of an arsenate detoxification pathway by DNA shuffling [8].. *After three rounds of DNA shuffling and ... and rabbit by DNA shuffling and screening [19].. *To select for chimeric Pgp with an altered resistance profile, DNA shuffling ...
  https://www.wikigenes.org/e/mesh/e/20923.html
*  DNA shuffling - Wikipedia
... hence the term DNA shuffling. Restriction enzymes that cut in similar places are used to digest members of the gene family DNA ... DNA shuffling is a way to rapidly propagate beneficial mutations in a directed evolution experiment. It is used to rapidly ... Cohen, J. (13 July 2001). "How DNA Shuffling Works". Science. 293 (5528): 237. doi:10.1126/science.293.5528.237. Bacher, Jamie ... 2002). "Anticipatory evolution and DNA shuffling" (PDF). Genome Biology. 3 (8). ...
  https://en.wikipedia.org/wiki/DNA_shuffling
*  Synthetic DNA Shuffling Enzyme Outpaces Natural Counterpart
... News Jun 22, 2018 , Original Press Release from the University of ... A synthetic DNA enzyme inserts into a cell membrane, causing lipids to shuffle between the inner and outer membrane layers. ... They saw that when certain DNA structures insert into the membrane - in this case, a bundle of eight strands of DNA with ... Aksimentiev's group came upon DNA's scramblase activity when looking at DNA structures that form pores and channels in cell ...
  https://www.technologynetworks.com/genomics/news/synthetic-dna-shuffling-enzyme-outpaces-natural-counterpart-305279
*  Improved Green Fluorescent Protein by Molecular Evolution Using DNA Shuffling - Semantic Scholar
DNA shuffling can be combined with screening of a moderate number of mutants. We envision that the combination of DNA shuffling ... After 3 cycles of DNA shuffling, a mutant was obtained with a whole cell fluorescence signal that was 45-fold greater than a ... We constructed a synthetic GFP gene with improved codon usage and performed recursive cycles of DNA shuffling followed by ... Expressed in Chinese Hamster Ovary (CHO) cells, this shuffled GFP mutant showed a 42-fold improvement over wildtype GFP ...
  https://www.semanticscholar.org/paper/Improved-Green-Fluorescent-Protein-by-Molecular-DNA-Crameri-Whitehorn/0048cf488ab4fd46b4b462b36b50d132f2478dbe
*  DNA enzyme shuffles cell membranes a thousand times faster than its natural counterpart
... ... DNA enzyme shuffles cell membranes a thousand times faster than its natural counterpart ... of Cambridge's Cavendish Laboratory and the University of Illinois at Urbana-Champaign say their lipid-scrambling DNA enzyme is ...
  https://www.phy.cam.ac.uk/news/dna-enzyme-shuffles-cell-membranes-a-thousand-times-faster-than-its-natural-counterpart
*  Stop GM Soya 356043!
DNA shuffling inherently hazardous Soybean 356043 involves a novel, untried technique called DNA- or gene-shuffling to create a ... As DNA shuffling can produce billions of recombinants in a matter of hours, it is unlikely that most of the recombinants will ... Death by DNA shuffling. Science in Society 18, 9, 2003.. *Pioneer Hi-Bred International Inc. Pioneer breaks new ground for ... The gene gat46014 enhanced using DNA shuffling, isolated from the bacterium Bacillus licheniformis, codes for an enzyme that ...
  http://www.i-sis.org.uk/stopGMsoya356043.php
*  Cytochrome P450 Protocols | SpringerLink
DNA Shuffling of Cytochrome P450 Enzymes James B. Y. H. Behrendorff, Wayne A. Johnston, Elizabeth M. J. Gillam ...
  https://link.springer.com/book/10.1007/978-1-62703-321-3
*  No Biosecurity without Biosafety
Death by DNA shuffling. Science in Society 2003, 18, 9-11. *Ho MW. Life after the Central Dogm series, Science in Society 2004 ... based in California invented a DNA shuffling technique in which genes, viral and bacterial genomes, whatever, could be chopped ... Generating millions of recombinant agents in hours by genome shuffling. *Creating huge varieties of vectors, DNA vaccines and ... They even suggest using environmental libraries, containing the DNA of the 99% bacteria unknown to science, in genome shuffling ...
  http://www.i-sis.org.uk/BiosecurityBiosafety.php
*  Antievolution.org - Antievolution.org Discussion Board -Topic::Co-option/change of function
DNA Shuffling It is possible that fewer than nine amino acid changes are required to interconvert melamine deaminase and ... In this context, DNA shuffling was conducted and the clonal variants were screened against a chemical library of substrates ... Here is a case of the origin of an autotransporter (AT) gene, lav by domain shuffling; I quote just a bit, the whole rather ... 10) Life Before DNA: The Origin and Evolution of Early Archean Cells. 11) The Prebiotic Evolution of Complex Molecules: A ...
  http://www.antievolution.org/cgi-bin/ikonboard/ikonboard.cgi?s=5b4d540d2f3a1737
*  Antievolution.org - Antievolution.org Discussion Board -Topic::Evolution of multiple-parts-required pathways
DNA Shuffling It is possible that fewer than nine amino acid changes are required to interconvert melamine deaminase and ... In this context, DNA shuffling was conducted and the clonal variants were screened against a chemical library of substrates ... was shown to hydrolyze ametryn, but it was not tested with atraton or other methoxy-functionalized herbicides (50). DNA from ... It is also of potential evolutionary significance that shuffled mutants were obtained with 80-fold enhanced activities with ...
  http://www.antievolution.org/cgi-bin/ikonboard/ikonboard.cgi?s=59ead8cb895728f7
*  Advanced Gene Therapeutics
DNA Shuffling. *Directed Design. Related Conference of Advanced Gene Therapeutics. Dec 03-04, 2018 ...
  https://www.omicsonline.org/conferences-list/advanced-gene-therapeutics
*  Directed evolution of ampicillin-resistant activity from a functionally unrelated DNA fragment: A laboratory model of molecular...
DNA shuffling was done as described (1). Fragments of 100 to 300 bp were used to reassemble the 1.2-kb DNA. The rate of ... The activity of this clone was enhanced by 50 rounds of directed evolution by using DNA shuffling. Analysis of the evolved DNA ... The sequence of the original "preevolved" DNA, AR0, is aligned with that of AR50-3. PCR primers used for DNA shuffling anneal ... The 1.2-kb fragment was amplified from the plasmid mix by using PCR, and a subsequent round of DNA shuffling was done to ...
  http://www.pnas.org/content/98/3/903
*  The virtue of innovation: innovation through the lenses of biological evolution | Journal of The Royal Society Interface
1994 DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution. Proc. Natl Acad. Sci ... 5.1.1. Systematic DNA sequencing. As is well known, following the development of systematic DNA sequencing [87-89], there was ... synthesis of fluorescent DNA primers for use in DNA sequence analysis. Nucleic Acids Res. 13, 2399-2412. (doi:10.1093/nar/13.7. ... 1977 DNA sequencing with chain-terminating inhibitors. Proc. Natl Acad. Sci. USA 74, 5463-5467. (doi:10.1073/pnas.74.12.5463). ...
  http://rsif.royalsocietypublishing.org/content/12/103/20141183?ijkey=ZNlI21r4A3AwLQi&keytype=ref
*  Predicting Evolutionary Potential: In Vitro Evolution Accurately Reproduces Natural Evolution of the TEM β-Lactamase | Genetics
2001 DNA shuffling method for generating highly recombined genes and evolved enzymes. Nat. Biotechnol. 19: 354-359. ... 1996 Improved green fluorescent protein by molecular evolution using DNA shuffling. Nat. Biotechnol. 14: 315-319. ... 1994 Rapid evolution of a protein in vitro by DNA shuffling. Nature 370: 389-390. ... 1986 Some probabilistic and statistical problems on the analysis of DNA sequences. Lect. Math. Life Sci. 17: 57-86. ...
  http://www.genetics.org/content/160/3/823
*  Lääketieteen ja biotieteiden tiedekunta
A novel chimeric avidin with increased thermal stability using DNA shuffling.. PLoS One ;9(3)e92058, 2014. PMID. ... A novel chimeric avidin with increased thermal stability using DNA shuffling.. PLoS One ;9(3)e92058, 2014. PMID. ... DNA sequences proximal to human mitochondrial DNA deletion breakpoints prevalent in human disease form G-quadruplexes, a class ... Hans Spelbrink - Molecular Biology-Mitochondrial DNA maintenance. Holt IJ, Jacobs HT. Unique features of DNA replication in ...
  http://www.uta.fi/bmt/julkaisut/2014_julkaisut.html
*  US5958751A - α-galactosidase - Google Patents
DNA shuffling to produce herbicide selective crops US6500639B2 (en) 1998-10-07. 2002-12-31. Maxygen, Inc.. DNA shuffling to ... Optimization of pest resistance genes using DNA shuffling US6500639B2 (en) 1998-10-07. 2002-12-31. Maxygen, Inc.. DNA shuffling ... Optimization of pest resistance genes using DNA shuffling US6605430B1 (en) 1998-08-12. 2003-08-12. Maxygen, Inc.. DNA shuffling ... which DNA includes cDNA, genomic DNA, and synthetic DNA. The DNA may be double-stranded or single-stranded, and if single ...
  https://patents.google.com/patent/US5958751A/en
*  Environmental changes bridge evolutionary valleys | Science Advances
Rapid evolution of a protein in vitro by DNA shuffling. Nature 370, 389-391 (1994).. ... and plasmid DNA was isolated. Amplicons were generated directly from each plasmid DNA prep. During PCR, 3-bp barcodes ... Plasmid DNA was isolated from the pelleted cells. Amplicons were prepared for deep sequencing via PCR from each plasmid prep ... Thus, by deep sequencing the plasmid DNA to determine the frequency of each allele on each plate, the fitness for each mutant ...
  http://advances.sciencemag.org/content/2/1/e1500921.full
*  IJMS | Free Full-Text | Computational Protein Engineering: Bridging the Gap between Rational Design and Laboratory Evolution |...
Stemmer, W.P. Rapid evolution of a protein in vitro by DNA shuffling. Nature 1994, 370, 389-391. [Google Scholar] ... and DNA shuffling [24] have essentially revolutionized biocatalysis [25], as they allow enzyme properties to be altered through ... Xiang, Y.; Oelschlaeger, P.; Florian, J.; Goodman, M.F.; Warshel, A. Simulating the effect of DNA polymerase mutations on ... catalysts as biomimics of RNA and DNA phosphoryl transfer enzymes: Changing the medium from water to aclohol provides enzyme- ...
  http://www.mdpi.com/1422-0067/13/10/12428/htm
*  Algorithms for Joint Optimization of Stability and Diversity in Planning Combinatorial Libraries of Chimeric Proteins |...
Stemmer, W.: Rapid evolution of a protein in vitro by DNA shuffling. Nature 370(6488), 389-391 (1994)CrossRefGoogle Scholar ... Coco, W.: RACHITT: Gene family shuffling by random chimeragenesis on transient templates. Methods Mol. Biol. 231, 111-127 (2003 ...
  https://link.springer.com/chapter/10.1007%2F978-3-540-78839-3_26
*  When Genes "Breed" In The Lab, A Suprising Number Of Their Offspring Are Supergenes | EurekAlert! Science News
A company in California has used a DNA shuffling technique to make interferons that appear to be 285 000 times as effective ... By comparison, 13 per cent of the enzymes Maxygen produced through DNA shuffling were superior. "DNA shuffling is a very ... Maxygen, a company in Santa Clara, California, has used a DNA shuffling technique to create interferons that are dramatically ... Using its system of DNA shuffling, Maxygen made 600 new daughter genes, 77 of which produced superior enzymes. Screening showed ...
  https://www.eurekalert.org/pub_releases/1998-11/NS-WGIT-181198.php
*  Chemtrail/Contrail Mystery Spraying Solved? Is This What's Going On?
DNA shuffling. DNA shuffling is a revolutionary method for directed evolution. Maxygen uses a preferred format, called family ... Improved Vaccines by DNA Shuffling of Pathogen Antigens U.S. OSIRIS Therapeutics, Inc. Baltimore, MD. Sequential Auto ... Maxygen's expertise in DNA shuffling and library screening technologies.. Pathogenic spore-forming bacteria like Bacillus ... Activation of Innate Immunity by CpG DNA for Broad Spectrum Protection Against Pathogens U.S. University of Texas-Southwestern ...
  http://netowne.com/environmental/contrails/sightings/mystery.htm