*  Genomic -Lactamase? Blue White screening not working - Molecular Cloning - BioForum
Im having some trouble regarding my cloning procedure. In the past we used to have some XL-Blue competent E.Coli for ... transform my bacteria and use blue-white screening to select positively transformed clones.... ... Now we switched to another commercial strain (http://tools.lifetec...h5alpha_man.pdf) For cloning I always subclone my PCR ... For cloning I always subclone my PCR products first into pGEM-T vector, transform my bacteria and use blue-white screening to ...
  http://www.protocol-online.org/forums/topic/32700-genomic-ss-lactamase-blue-white-screening-not-working/
*  Help cloning!!! - Molecular Cloning - BioForum
... posted in Molecular Cloning: Hello every body I hope someone can gimme a clue! Problem: I need to clone a fragment of PCR of ... I analyzed three clones and if they look bigger than the original plasmid I analyzed them by fast digestion with BamHI and XhoI ... This makes me think I shoud have the product at least, but I can not find a single colony bearing the clone!!!. Anyone can help ... Problem: I need to clone a fragment of PCR of about 4.2 Kb in a plasmid of about 5.3Kb. Oligos are containing 2 different ER ...
  http://www.protocol-online.org/forums/topic/7949-help-cloning/
*  New Yeast Cloning System for Producing Proteins with Native Amino Acid,,,Sequences ( One-column protein purification and n...)
Cloning PCR products using the LIC strategy is so efficient that as li...,New,Yeast,Cloning,System,for,Producing,Proteins,with, ... One-column protein purification and native eukaryotic amino acid se... figure 1 ...Stratagenes ESP LIC cloning and expression ... Cloning PCR products using the LIC strategy is so efficient that as little as 20 ng of PCR product is required for each cloning ... Enhanced PCR Cloning System. 7. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning. 8. ...
  http://www.bio-medicine.org/biology-technology/New-Yeast-Cloning-System-for-Producing-Proteins-with-Native-Amino-Acid-0D-0A-0ASequences-44-1/
*  Cloning of GFP - Molecular Cloning - BioForum
... posted in Molecular Cloning: Hi there, So I recently ligated my gene into a Pet32a vector using restriction sites using EcoR1 ... Cloning of GFP. Started by biology_06er, Oct 23 2011 02:42 AM ...
  http://www.protocol-online.org/forums/topic/23184-cloning-of-gfp/
*  Molecular Cloning and Biological Characterization of a Novel Murine Lymphoid Growth Factor | JEM
The extent of each clone with reference to Fig. 1 is: clone 17, nucleotides 7-750; clone 2-1, nucleotides 1-750; clone 1A, ... Molecular Cloning and Biological Characterization of a Novel Murine Lymphoid Growth Factor. John E. Sims, Douglas E. Williams, ... cDNA Cloning.. RNA was isolated from Z210R.1 cells, oligo-dT-primed cDNA was synthesized and cloned into the pDC406 vector, ... Molecular Cloning and Biological Characterization of a Novel Murine Lymphoid Growth Factor ...
  http://jem.rupress.org/content/192/5/671
*  Gene Cloning: A breakthrough - Orbit Biotech
By obpl, 2017-12-04T16:54:41+00:00 December 19th, 2016,Molecular Biology,Comments Off on Gene Cloning: A breakthrough ... Cell-based gene cloning is an in-vivo cloning method while cell-free gene cloning is an in vitro cloning method which involves ... Essentially, two different gene cloning approaches are used: cell-based gene cloning and cell free DNA cloning. ... cloning of penicillin G acylase gene; cloning of Hepatitis B virus genome; cloning of genes for human peptide hormones (insulin ...
  http://orbitbiotech.com/gene-cloning-a-breakthrough/
*  Cloning Mitochondrial Targeting Sequence - Molecular Cloning - BioForum
... posted in Molecular Cloning: Dear All,I'm actually having problems to clone a 95 bp fragment into a 7kbp vector.I ordered the ... Only one clone showed a band at approx. 100 bp. I sent this clone for sequencing. Surprisingly there was a 65 bp insert between ... I'm actually having problems to clone a 95 bp fragment into a 7kbp vector.. I ordered the dsDNA 95 bp oligo, containing a ... Cloning Mitochondrial Targeting Sequence. Started by speci, Apr 27 2013 11:34 AM ...
  http://www.protocol-online.org/forums/topic/29143-cloning-mitochondrial-targeting-sequence/
*  cloning vector,blunt zero cloning,pEASY®-Blunt Zero Cloning Kit,Cloning Vectors,Beijing TransGe
Cloning Vectors,Cloning and Mutagenesis System,Products,Beijing TransGen Biotech Co.Ltd,OverviewContents& storageCitations & ... Molecular cloning and characterization of the mitogen-activated protein kinase kinase gene (MKK4) and its promoter sequence ... Your present location:Home / Products / Cloning and Mutagenesis System / Cloning Vectors / pEASY®-Blunt Zero Cloning Kit ... pEASY ®-Blunt Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells ...
  http://www.transbionovo.com/rx_detail/productId=208.html
*  PCR Cloning Kits (Lyophilized) - In-Fusion HD Plus EcoDry Cloning System
In-Fusion EcoDry PCR Cloning Kits allow you to clone any PCR fragment into any linearized vector in a single step without ... Molecular Weight Markers * DNA Molecular Weight Standard Markers * Protein Molecular Weight Markers ... Cloning Kits (Lyophilized)-In-Fusion HD Plus EcoDry Cloning System. In-Fusion HD EcoDry Cloning Plus kits are provided in a ... Cloning using modular parts *High-throughput cloning. Choose the Right In-Fusion Cloning Plus System for Your Needs. ...
  http://www.clontech.com/US/Products/Cloning_and_Competent_Cells/Cloning_Kits/In-Fusion_EcoDry?sitex=10020:22372:US&PROD=RawSN17J9Fp2oAxvmkFXFdZJ:S&PROD_pses=ZGB9FA2D2323EAE290497433061737DC58E453A4B9EF65AC2478E574DB6A0C7D9B01B61B0D20B410577EB804F55DCE6488E58AA1235FFED08F
*  cloning trouble - Molecular Cloning - BioForum
... posted in Molecular Cloning: Hello all, I'm quite new at cloning.. i'm using a kit from invitrogen. the pcr product i want to ... clone is 5 kb and i'm using chemically competent cells which i've stored in a -80 freezer. My first two trials yielded no ... Hello all, I'm quite new at cloning.. i'm using a kit from invitrogen. the pcr product i want to clone is 5 kb and i'm using ... Honestly, I am not very clear how are you trying to clone your insert, is it TA cloning? or which other kit are you using? ...
  http://www.protocol-online.org/forums/topic/6967-cloning-trouble/
*  A Modified MultiSite Gateway Cloning Strategy for Consolidation of Genes in Plants | SpringerLink
Molecular Biotechnology. February 2013. , Volume 53, Issue 2, pp 129-138 , Cite as ... 2000). GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes. Methods ... In this study, we modified gateway MultiSite cloning vectors and developed a hybrid cloning strategy which combines advantages ... Gene cloning vectors that facilitate the fusion, overexpression or down regulation of genes in plant cells are being used with ...
  https://link.springer.com/article/10.1007%2Fs12033-012-9499-6
*  How to design RT-PCR primers in-frame for expression vector? - Molecular Cloning - BioForum
... posted in Molecular Cloning: I have a few sets of primers from a paper, for PCR amplifcation of cDNA. I want to use these ... Protocols and Techniques Forums → Molecular CloningMolecular cloning into pGhost5 Started by Lana, 07 Apr 2017 cloning * 0 ... Protocols and Techniques Forums → Molecular Cloning → Help with making a Dimer Started by kenny17, 09 Jul 2017 cloning, ssDNA, ... Protocols and Techniques Forums → Molecular Biology → Odd issue converting mutiplex PCR to single reactions Started by ...
  http://www.protocol-online.org/forums/topic/24673-how-to-design-rt-pcr-primers-in-frame-for-expression-vector/
*  Molecular cloning - Wikipedia
Obtaining the molecular clone of a gene can lead to the development of organisms that produce the protein product of the cloned ... In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated ... Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and ... In standard molecular cloning experiments, the cloning of any DNA fragment essentially involves seven steps: (1) Choice of host ...
  https://en.wikipedia.org/wiki/Molecular_cloning
*  Molecular Cloning - BioForum
Javascript Disabled Detected You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.. ...
  http://www.protocol-online.org/forums/videos/category-2-molecular-cloning/
*  cloning - Molecular Cloning
Top : Forum Archives: : Molecular Cloning. cloning - (Jan/14/2008 ). I am trying to PCR the digested and dephosphorylated ...
  http://www.protocol-online.org/biology-forums/posts/33391.html
*  help - Molecular Cloning
Top : Forum Archives: : Molecular Cloning. help - (Nov/26/2007 ). We've construct the cDNA library by using CloneMiner cDNA ...
  http://www.protocol-online.org/biology-forums/posts/32355.html
*  Cloning - Molecular Biology
Top : Forum Archives: : Molecular Biology. Cloning - RE digestion clean up (Jan/06/2006 ). Hi. Usually I lost much DNA when ...
  http://www.protocol-online.org/biology-forums/posts/12527.html
*  Transformation - Molecular Cloning - BioForum
... posted in Molecular Cloning: Hello I am using a geneart product (synthesized gene of my interest in a lenti back bone). They ... you should check the DNA they sent you for high molecular DNA that stays in the pockets on an agarose gel).. Another reason for ...
  http://www.protocol-online.org/forums/topic/23698-transformation/
*  re-digestion - Molecular Cloning
Top : New Forum Archives (2009-): : Molecular Cloning. re-digestion - (Mar/18/2010 ). Hi everyone. I am trying to do cloning. I ... I am trying to do cloning. I double digested my PCR products and vector (which had another insert). I have seen that this other ...
  http://www.protocol-online.org/biology-forums-2/posts/13791.html
*  KanR sequence - Molecular Cloning
Top : New Forum Archives (2009-): : Molecular Cloning. KanR sequence - (Mar/07/2012 ). What is the minimal cassette for ... I don't have other vectors for KanR... Well, I have one with AmpR, so I could clone the other way round (AmpR into KanR). But ... Depending on where you decided to clone, you may have omitted a 5' UP element from the sequence, 5' of the -35 region. In ... but it does not seem to confer KanR when I clone it into an AmpR plasmid. Is there something missing, or is it too big?. Any ...
  http://www.protocol-online.org/biology-forums-2/posts/24702.html
*  Cloning advice - Molecular Cloning
Top : New Forum Archives (2009-): : Molecular Cloning. Cloning advice - (May/21/2013 ). I clone a 915bp genes into PCDNA3. ... sometimes you lose things during cloning. typically there is something unanticipated like a recombination event or poor primer ... do you have other colonies you picked from the cloning to sequence? ...
  http://www.protocol-online.org/biology-forums-2/posts/29332.html
*  blunt-cloning - Molecular Cloning
Top : Forum Archives: : Molecular Cloning. blunt-cloning - all are vector self-ligation products? (Jul/11/2003 ). Pages: ... I do blunt cloning directly from PCR products and don't really have problems. MMM...If CIP is not working very well maybe try ... I am doing a very tricky blunt-end cloning trying to get a 3 kb fragment into a 12 kb vector. Maybe the dephosphorylation makes ...
  http://www.protocol-online.org/biology-forums/posts/2540more1.html
*  ligation inhibitors - Molecular Cloning
Top : Forum Archives: : Molecular Cloning. ligation inhibitors - (Mar/22/2006 ). I'm having problems with a ligation.. I've ... the DNA fragment which I'm cloning is a gene fusion with the GFP. Can the GFP (N-terminal fused) be toxic for the E .coli ...
  http://www.protocol-online.org/biology-forums/posts/14860.html
*  Transformation troubleshoot - Molecular Cloning
Top : Forum Archives: : Molecular Cloning. Transformation troubleshoot - (Jul/22/2008 ). Hi, I'm trying to isolate a plasmid ... I would suspect that you will see a lot of large molecular weight material that would correspond to the genomic DNA, again it ...
  http://www.protocol-online.org/biology-forums/posts/38243.html
*  Restriction digest - Molecular Cloning
Top : New Forum Archives (2009-): : Molecular Cloning. Restriction digest - (Oct/22/2009 ). Hi, I am new to do research. I ... Is this plasmid DNA that has been cloned in E. coli? Genomic DNA or plasmids from other species can contain modifications that ...
  http://www.protocol-online.org/biology-forums-2/posts/11043.html