Patent US7384738 - Retrovirus-based genomic screening - Google Patents
... or artificial chromosome vector). The resulting vector (or library of vectors containing a plurality of independent genomic ... such as yeast artificial chromosomes (YACs), and bacterial artificial chromosomes (BACs, Shizuya et al. 1992, Pro. Natl. Acad. ... human lung cells (W138, ATCC CCL 75); human liver cells (Hep G2, HB 8065); mouse mammary tumor (MMT 060562, ATCC CCL51); TRI ... yeast artificial chromosome, and bacterial artificial chromosome vectors. In some embodiments, the genomic DNA sequence is from ...http://www.google.com/patents/US7384738?dq=5,966,702
Retargeting of microcell fusion towards recipient cell-oriented transfer of human artificial chromosome | BMC Biotechnology |...
Human artificial chromosomeMeasles Virus fusogenic proteinChimeric protein. Background. Microcell-mediated chromosome transfer ... Human artificial chromosome (HAC) vector with a conditional centromere for correction of genetic deficiencies in human cells. ... Human artificial chromosome (HAC) vector provides long-term therapeutic transgene expression in normal human primary ... A gene delivery system with a human artificial chromosome vector based on migration of mesenchymal stem cells towards human ...https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12896-015-0142-z
Artificial chromosome vector - The General Hospital Corporation
Human DNA was prepared by lysis of monolayers of HeLa cells with 100 mM Tris-HCl pH 8.0, 50 mM EDTA, 500 mM NaCl, 1% SDS, and ... In addition, development of artificial plant chromosome vectors will facilitate the construction of artificial chromosomes that ... artificial chromosomes. The ability to construct artificial chromosomes that function in yeast, however, does not teach one ... Artificial chromosomes participate in homologous recombination preferentially. Since the artificial chromosomes remain intact ...http://www.freepatentsonline.com/5270201.html
Human artificial chromosome - Wikipedia
Yeast artificial chromosomes and bacterial artificial chromosomes were created before human artificial chromosomes, which first ... A human artificial chromosome (HAC) is a microchromosome that can act as a new chromosome in a population of human cells. That ... In 2010, a refined human artificial chromosome called 21HAC was reported. 21HAC is based on a stripped copy of human chromosome ... "A new chromosome 14-based human artificial chromosome (HAC) vector system for efficient transgene expression in human primary ...https://en.wikipedia.org/wiki/Human_artificial_chromosome
Case Westerners Construct First Artificial Human Chromosomes | BioWorld
BioWorld Online is the news service of record for the biotechnology industry and is updated every business morning. BioWorld Online will keep you up to date on all of the industry's business, science and regulatory news -- mergers and collaborations, FDA hearings and results, breakthroughs in research and much more.http://www.bioworld.com/content/case-westerners-construct-first-artificial-human-chromosomes
Human artificial chromosome - wikidoc
Yeast artificial chromosomes and bacterial artificial chromosomes were invented before human artificial chromosomes, which ... A human artificial chromosome (short HAC) is a microchromosome that can act as a new chromosome in a population of human cells ... They are useful in expression studies as gene transfer vectors and are a tool for elucidating human chromosome function. Grown ... Formation of de novo centromeres and construction of first-generation human artificial microchromosomes in Nature Genetics 15, ...https://www.wikidoc.org/index.php/Human_artificial_chromosome
HSV-1 delivery of artificial chromosomes to human cells - Physiology, Anatomy and Genetics
Expression of human smooth muscle calponin in transgenic mice revealed with a bacterial artificial chromosome | Heart and...
Subsequent reports using either yeast artificial chromosomes, bacterial artificial chromosomes (BACs), or P1 phage artificial ... Expression of human smooth muscle calponin in transgenic mice revealed with a bacterial artificial chromosome. Joseph M. Miano ... 1997) Human apolipoprotein B transgenic mice generated with 207- and 145-kilobase pair bacterial artificial chromosomes: ... Expression of human smooth muscle calponin in transgenic mice revealed with a bacterial artificial chromosome ...http://ajpheart.physiology.org/content/282/5/H1793.long
Genes | Free Full-Text | The Past, Present, and Future of Human Centromere Genomics
... assembly that have provided important insight into the abundance and complex organization of this sequence on human chromosomes ... Human centromeres are located at repetitive alpha satellite DNA arrays that compose approximately 5% of the genome. Contiguous ... In light of these new findings, we offer perspectives for future studies of human centromere assembly and function. ... centromere genomics spanning the discovery of the sequence to its molecular characterization and the work done during the Human ...http://www.mdpi.com/2073-4425/5/1/33
Forbidden flavors: scientists consider how disgusting tastes can linger surreptitiously in memory. - Free Online Library
Human artificial chromosome created.. Topics:. Animal learning. Research. Aversive stimuli. Research. Food habits ... Garcia's research also inspires psychologists now investigating central realms of human knowledge and learning, such as ... especially for humans." For his part, Garcia classifies the effect that he has championed as simply one of many "instincts for ... humans show comparable taste aversions if nauseating injections are delivered during sleep. Garcia describes this research in ...https://www.thefreelibrary.com/Forbidden+flavors%3A+scientists+consider+how+disgusting+tastes+can+...-a019308368
Table of Contents - May 08, 2001, 98 (10) | PNAS
Neocentromeres and human artificial chromosomes: An unnatural act Huntington F. Willard. PNAS May 8, 2001 98 (10) 5374-5376; ... Sequence conservation at human and mouse orthologous common fragile regions, FRA3B/FHIT and Fra14A2/Fhit Takeshi Shiraishi, ... Y chromosome polymorphism is a strong determinant of male fitness in Drosophila melanogaster Adam K. Chippindale and William R ... Involvement of a human gene related to the Drosophila spen gene in the recurrent t(1;22) translocation of acute megakaryocytic ...http://www.pnas.org/content/98/10
A new assay for measuring chromosome instability (CIN) and identification of drugs that elevate CIN in cancer cells | BMC...
This quantitative assay for chromosome mis-segregation is based on the use of a non-essential human artificial chromosome (HAC ... Using the HAC-based chromosome loss assay, we have analyzed several well-known anti-mitotic, spindle-targeting compounds, all ... The identification of new compounds that increase chromosome mis-segregation rates should expedite the development of new ... this new and simple assay allows for a quick and efficient screen of hundreds of drugs to identify those affecting chromosome ...https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-13-252
Call for abstract | Synthetic Biology Conference | Computational Biology | Worldwide events | Singapore | Asia Pacific | USA |...
Track 4:Mammalian Synthetic Biology (Human Artificial Chromosomes) Scientists tend to engineer human cells to remember past ... Track 3:Artificial DNA synthesis Artificial DNA synthesis, sometimes known as DNA printing is a method in synthetic biology ... In contrast, the previous Sanger sequencing technology, used to decipher the human genome, required over a decade to deliver ... Methods in genome engineering are: Insertion involves introducing a gene into a chromosome to obtain a new function. ...https://syntheticbiology.conferenceseries.com/asiapacific/2017/call-for-abstracts.php
Bacterial Artificial Chromosome Mutagenesis Using Recombineering
kb-alphoid DNA into a human BAC clones via the λ Red recombineering system in an attempt to build human artificial chromosomes ... Construction of human artificial chromosome vectors by recombineering. Gene. 2005;351:29-38. [PubMed] ... Efficient and precise engineering of a 200 kb β-globin human/bacterial artificial chromosome in E. coli DH10B using an ... Human apolipoprotein B transgenic mice generated with 207- and 145- kilobase pair bacterial artificial chromosomes. Evidence ...http://pubmedcentralcanada.ca/pmcc/articles/PMC3005948/
Chromosome Centromeres Are Inherited Epigenetically - Redorbit
This discovery may help to further the development of artificial human chromosomes, which could be used for gene therapies in ... Scientists would like to develop artificial human chromosomes as an alternative to gene therapy using viruses. "Like their ... During cell division, the kinetochore provides a point of attachment for the cell skeleton and enables the chromosomes to move ... The cell skeleton, which distributes the chromosomes to the two daughter cells during cell division, attaches to the ...http://www.redorbit.com/news/science/1112415890/chromosome-centromeres-are-inherited-epigenetically/
Centromeric Localization and Adaptive Evolution of an Arabidopsis Histone H3 Variant | Plant Cell
Willard, H.F. (1998). Centromeres: The missing link in the development of human artificial chromosomes. Curr. Opin. Genet. Dev. ... In pachytene chromosomes, we found weak signal over the euchromatic arms of the chromosomes, but not over heterochromatic ... Haaf, T., and Willard, H.F. (1997). Chromosome-specific alpha-satellite DNA from the centromere of chimpanzee chromosome 4. ... and spots could be seen to lie centrally on chromosomes (Figure 3B). The spots were on the leading portions of the chromosomes ...http://www.plantcell.org/content/14/5/1053.long
Meetings of GC-Lab & Harvard Molecular Technology Group
Jun 29, 207 Thu Guest Speaker: Alina Chan; Title: 'human artificial chromosome that shuttles the three kingdoms' Rigel Chan ... On-chip human microvascular models for the study of hematogenous dissemination of cancer' ... On-chip human microvascular models for the study of hematogenous dissemination of cancer' ... Aug 10, 2015 Mon George Chao ; Eriona Hysolli, Guest speaker, Presentation Title: 'Novel regulators of human somatic cell ...http://arep.med.harvard.edu/groupmeeting.html
Doctoral oral exams for the week of Jan. 31-Feb. 4 | Office of News & Media Relations | UMass Amherst
Dissertation: "Fully human antigen-specific polyclonal antibody responses induced in cloned human artificial chromosome ...http://www.umass.edu/newsoffice/article/doctoral-oral-exams-week-jan-31-feb-4
Wound healing properties of jojoba liquid wax: An in vitro study.
... were studied in vitro on HaCaT keratinocytes and human dermal fibroblasts, which are involved in wounded skin repair. MATERIALS ... 8756669 - Stable episomal maintenance of yeast artificial chromosomes in human cells.. 2543159 - Detection of hepatitis a virus ... 19394129 - A two-step strategy for neuronal differentiation in vitro of human dental follicle cells.. ... 19706449 - Conversion of mdck cell line to suspension culture by transfecting with human siat7e ge.... ...http://www.biomedsearch.com/nih/Wound-healing-properties-jojoba-liquid/21211559.html
Anti-Nucleolin antibody [4E2] - ChIP Grade (ab13541) References
References for Human in ChIP, ICC/IF, IF, IHC-P, WB ... Wakai M et al. A human artificial chromosome recapitulates the ... Sato H et al. Identification of nucleolin as a protein that binds to human papillomavirus type 16 DNA. Biochem Biophys Res ... Munday DC et al. Quantitative proteomic analysis of A549 cells infected with human respiratory syncytial virus. Mol Cell ... Rana R & Biegalke BJ Human cytomegalovirus UL34 early and late proteins are essential for viral replication. Viruses 6:476-88 ( ...http://www.abcam.com/nucleolin-antibody-4e2-chip-grade-ab13541-references.html
Frontiers | Induced pluripotent stem cells: applications in regenerative medicine, disease modeling, and drug discovery | Cell...
They used Human Artificial Chromosome (HAC) for the expression of complete sequence of Dystrophin (DYS). They used fibroblasts ... Further research conducted on human stem cells (HSCs) made burgeoning use of human ESCs for which embryo needed to be isolated ... 2010). Generation of functional human hepatic endoderm from human induced pluripotent stem cells. Hepatology 51, 329-335. doi: ... a newly discovered drug or therapy must be tested on human cells or human test models itself. These reasons make it more ...https://www.frontiersin.org/articles/10.3389/fcell.2015.00002/full
Case Western Reserve University School of Medicine - Wikipedia
1997 - Team led by Professor Huntington Willard (Chair of Genetics) create world's first artificial human chromosome. M. Scott ... of the world's first human artificial chromosome. Integrated MD-PhD Training: In 1956, CWRU School of Medicine began an ... 1975 - Discovery that human renin, an enzyme produced by the kidney, is involved in hypertension 1990 - National team led by ... Research History: Development of the modern technique for human blood transfusion using a cannula to connect blood vessels; ...https://en.wikipedia.org/wiki/Case_Western_Reserve_University_School_of_Medicine
Human antibodies derived from immunized xenomice - Abgenix, Inc.
Fully human antibodies against a specific antigen can be prepared by administering the antigen to a transgenic animal which has ... Garza, et al., "Mapping the drosophila genome with yeast artificial chromosomes with yeast artificial chromosomes", Science 246 ... human TNFα, human CD4, human L-selectin, human PTHrp and human gp39. Antibodies and analogs immunoreactive with human TNFα and ... Huxley, et al., "The human HPRT gene on a yeast artificial chromosome is functional when transferred to mouse cells by cell ...http://www.freepatentsonline.com/6150584.html
Combinatorial depletion analysis to assemble the network architecture of the SAGA and ADA chromatin remodeling complexes |...
Sardiu ME, Cai Y, Jin J, Swanson SK, Conaway RC, Conaway JW, Florens L, Washburn MP (2008) Probabilistic assembly of human ... The suppression of VP16 toxicity in ADA mutants is accompanied by the inability to activate an artificial LacZ reporter gene ... Functional dissection of protein complexes involved in yeast chromosome biology using a genetic interaction map. Nature 446: ... such as human keratins, IgGs and proteolytic enzymes) and, to estimate false discovery rates (FDR), 5993 randomized sequences ...http://msb.embopress.org/content/7/1/503
Download Probabilistic Models For Nonlinear Partial Differential Equations
This chromosome number will require to be SSD. In download probabilistic models for nonlinear to verify out of this warning ... same download probabilistic, mine has human in the manufacturing of the angle. I lead to use and Make, but it will not be me. ... Whitetopping and artificial download probabilistic models. first project field; consequence air and membrane project in ... humans IN CIVIL ENGINEERING WITH LAB. drugs of biting download probabilistic models for nonlinear partial in the quot of ...http://pd19.org/ebook/download-probabilistic-models-for-nonlinear-partial-differential-equations