*  Patent US7384738 - Retrovirus-based genomic screening - Google Patents
... or artificial chromosome vector). The resulting vector (or library of vectors containing a plurality of independent genomic ... such as yeast artificial chromosomes (YACs), and bacterial artificial chromosomes (BACs, Shizuya et al. 1992, Pro. Natl. Acad. ... human lung cells (W138, ATCC CCL 75); human liver cells (Hep G2, HB 8065); mouse mammary tumor (MMT 060562, ATCC CCL51); TRI ... yeast artificial chromosome, and bacterial artificial chromosome vectors. In some embodiments, the genomic DNA sequence is from ...
  http://www.google.com/patents/US7384738?dq=5,966,702
*  Artificial chromosome vector - The General Hospital Corporation
Human DNA was prepared by lysis of monolayers of HeLa cells with 100 mM Tris-HCl pH 8.0, 50 mM EDTA, 500 mM NaCl, 1% SDS, and ... In addition, development of artificial plant chromosome vectors will facilitate the construction of artificial chromosomes that ... artificial chromosomes. The ability to construct artificial chromosomes that function in yeast, however, does not teach one ... Artificial chromosomes participate in homologous recombination preferentially. Since the artificial chromosomes remain intact ...
  http://www.freepatentsonline.com/5270201.html
*  Human artificial chromosome - Wikipedia
Yeast artificial chromosomes and bacterial artificial chromosomes were created before human artificial chromosomes, which first ... A human artificial chromosome (HAC) is a microchromosome that can act as a new chromosome in a population of human cells. That ... In 2010, a refined human artificial chromosome called 21HAC was reported. 21HAC is based on a stripped copy of human chromosome ... "A new chromosome 14-based human artificial chromosome (HAC) vector system for efficient transgene expression in human primary ...
  https://en.wikipedia.org/wiki/Human_artificial_chromosome
*  Case Westerners Construct First Artificial Human Chromosomes | BioWorld
BioWorld Online is the news service of record for the biotechnology industry and is updated every business morning. BioWorld Online will keep you up to date on all of the industry's business, science and regulatory news -- mergers and collaborations, FDA hearings and results, breakthroughs in research and much more.
  http://www.bioworld.com/content/case-westerners-construct-first-artificial-human-chromosomes
*  HSV-1 delivery of artificial chromosomes to human cells - Physiology, Anatomy and Genetics
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we will assume that you are happy to receive all cookies and you will not see this message again. Click 'Find out more' for information on how to change your cookie settings ...
  https://www.dpag.ox.ac.uk/publications/53579
*  Retargeting of microcell fusion towards recipient cell-oriented transfer of human artificial chromosome | BMC Biotechnology |...
Human artificial chromosomeMeasles Virus fusogenic proteinChimeric protein. Background. Microcell-mediated chromosome transfer ... Human artificial chromosome (HAC) vector with a conditional centromere for correction of genetic deficiencies in human cells. ... Human artificial chromosome (HAC) vector provides long-term therapeutic transgene expression in normal human primary ... A gene delivery system with a human artificial chromosome vector based on migration of mesenchymal stem cells towards human ...
  https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12896-015-0142-z
*  Expression of human smooth muscle calponin in transgenic mice revealed with a bacterial artificial chromosome | Heart and...
Subsequent reports using either yeast artificial chromosomes, bacterial artificial chromosomes (BACs), or P1 phage artificial ... Expression of human smooth muscle calponin in transgenic mice revealed with a bacterial artificial chromosome. Joseph M. Miano ... 1997) Human apolipoprotein B transgenic mice generated with 207- and 145-kilobase pair bacterial artificial chromosomes: ... Expression of human smooth muscle calponin in transgenic mice revealed with a bacterial artificial chromosome ...
  http://ajpheart.physiology.org/content/282/5/H1793.long
*  Forbidden flavors: scientists consider how disgusting tastes can linger surreptitiously in memory. - Free Online Library
Human artificial chromosome created.. Topics:. Animal learning. Research. Aversive stimuli. Research. Food habits ... Garcia's research also inspires psychologists now investigating central realms of human knowledge and learning, such as ... especially for humans." For his part, Garcia classifies the effect that he has championed as simply one of many "instincts for ... humans show comparable taste aversions if nauseating injections are delivered during sleep. Garcia describes this research in ...
  https://www.thefreelibrary.com/Forbidden+flavors%3A+scientists+consider+how+disgusting+tastes+can+...-a019308368
*  Wound healing properties of jojoba liquid wax: An in vitro study.
... were studied in vitro on HaCaT keratinocytes and human dermal fibroblasts, which are involved in wounded skin repair. MATERIALS ... 8756669 - Stable episomal maintenance of yeast artificial chromosomes in human cells.. 2543159 - Detection of hepatitis a virus ... 19394129 - A two-step strategy for neuronal differentiation in vitro of human dental follicle cells.. ... 19706449 - Conversion of mdck cell line to suspension culture by transfecting with human siat7e ge.... ...
  http://www.biomedsearch.com/nih/Wound-healing-properties-jojoba-liquid/21211559.html
*  Case Western Reserve University School of Medicine - Wikipedia
1997 - Team led by Professor Huntington Willard (Chair of Genetics) create world's first artificial human chromosome. M. Scott ... of the world's first human artificial chromosome. Integrated MD-PhD Training: In 1956, CWRU School of Medicine began an ... 1975 - Discovery that human renin, an enzyme produced by the kidney, is involved in hypertension 1990 - National team led by ... Research History: Development of the modern technique for human blood transfusion using a cannula to connect blood vessels; ...
  https://en.wikipedia.org/wiki/Case_Western_Reserve_University_School_of_Medicine
*  Cloning vector - Wikipedia
Insert of up to 3,000 kb may be carried by yeast artificial chromosome. Human artificial chromosome may be potentially useful ... "Human artificial chromosome (HAC) vector with a conditional centromere for correction of genetic deficiencies in human cells". ... Kouprina N, Earnshaw WC, Masumoto H, Larionov V (2013). "A new generation of human artificial chromosomes for functional ... Insert size of up to 350 kb can be cloned in bacterial artificial chromosome (BAC). BACs are maintained in E. coli with a copy ...
  https://en.wikipedia.org/wiki/Cloning_vector
*  Call for abstract | Synthetic Biology Conference | Computational Biology | Worldwide events | Singapore | Asia Pacific | USA |...
Track 4:Mammalian Synthetic Biology (Human Artificial Chromosomes) Scientists tend to engineer human cells to remember past ... Track 3:Artificial DNA synthesis Artificial DNA synthesis, sometimes known as DNA printing is a method in synthetic biology ... In contrast, the previous Sanger sequencing technology, used to decipher the human genome, required over a decade to deliver ... Methods in genome engineering are: Insertion involves introducing a gene into a chromosome to obtain a new function. ...
  https://syntheticbiology.conferenceseries.com/asiapacific/2017/call-for-abstracts.php
*  Bacterial Artificial Chromosome Mutagenesis Using Recombineering
kb-alphoid DNA into a human BAC clones via the λ Red recombineering system in an attempt to build human artificial chromosomes ... Construction of human artificial chromosome vectors by recombineering. Gene. 2005;351:29-38. [PubMed] ... Efficient and precise engineering of a 200 kb β-globin human/bacterial artificial chromosome in E. coli DH10B using an ... Human apolipoprotein B transgenic mice generated with 207- and 145- kilobase pair bacterial artificial chromosomes. Evidence ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC3005948/
*  Chromosome Centromeres Are Inherited Epigenetically - Redorbit
This discovery may help to further the development of artificial human chromosomes, which could be used for gene therapies in ... Scientists would like to develop artificial human chromosomes as an alternative to gene therapy using viruses. "Like their ... During cell division, the kinetochore provides a point of attachment for the cell skeleton and enables the chromosomes to move ... The cell skeleton, which distributes the chromosomes to the two daughter cells during cell division, attaches to the ...
  http://www.redorbit.com/news/science/1112415890/chromosome-centromeres-are-inherited-epigenetically/
*  Centromeric Localization and Adaptive Evolution of an Arabidopsis Histone H3 Variant | Plant Cell
Willard, H.F. (1998). Centromeres: The missing link in the development of human artificial chromosomes. Curr. Opin. Genet. Dev. ... In pachytene chromosomes, we found weak signal over the euchromatic arms of the chromosomes, but not over heterochromatic ... Haaf, T., and Willard, H.F. (1997). Chromosome-specific alpha-satellite DNA from the centromere of chimpanzee chromosome 4. ... and spots could be seen to lie centrally on chromosomes (Figure 3B). The spots were on the leading portions of the chromosomes ...
  http://www.plantcell.org/content/14/5/1053.long
*  YAC Transfer into Mammalian Cells by Cell Fusion | Springer for Research & Development
The large regions of DNA that can be cloned in yeast artificial chromosomes (YACs) are ideal for expression studies of the ... Huxley, C., Hagino, Y., Schlessinger, D., and Olson, M. V. (1991) The human HPRT gene on a yeast artificial chromosome is ... Pachnis, V., Pevny, L., Rothstein, R., and Costantini, F. (1990) Transfer of a yeast artificial chromosome carrying human DNA ... 1993) Creation of mice expressing human antibody light chains by introduction of a yeast artificial chromosome containing the ...
  https://rd.springer.com/protocol/10.1385/0-89603-313-9%3A281
*  Meetings of GC-Lab & Harvard Molecular Technology Group
Jun 29, 207 Thu Guest Speaker: Alina Chan; Title: 'human artificial chromosome that shuttles the three kingdoms' Rigel Chan ... On-chip human microvascular models for the study of hematogenous dissemination of cancer' ... On-chip human microvascular models for the study of hematogenous dissemination of cancer' ... Aug 10, 2015 Mon George Chao ; Eriona Hysolli, Guest speaker, Presentation Title: 'Novel regulators of human somatic cell ...
  http://arep.med.harvard.edu/groupmeeting.html
*  Anti-Nucleolin antibody [4E2] - ChIP Grade (ab13541) References
References for Human in ChIP, ICC/IF, IF, IHC-P, WB ... Wakai M et al. A human artificial chromosome recapitulates the ... Sato H et al. Identification of nucleolin as a protein that binds to human papillomavirus type 16 DNA. Biochem Biophys Res ... Munday DC et al. Quantitative proteomic analysis of A549 cells infected with human respiratory syncytial virus. Mol Cell ... Rana R & Biegalke BJ Human cytomegalovirus UL34 early and late proteins are essential for viral replication. Viruses 6:476-88 ( ...
  http://www.abcam.com/nucleolin-antibody-4e2-chip-grade-ab13541-references.html
*  Laurent Susini - Wikipedia
Mapping the whole human genome by fingerprinting yeast artificial chromosomes. Cell. 1992 Sep 18;70(6):1059-68. Cited 197 times ... "Mapping the whole human genome by fingerprinting yeast artificial chromosomes". Cell. 70 (6): 1059-68. doi:10.1016/0092-8674(92 ... "Mapping the whole human genome by fingerprinting yeast artificial chromosomes". Cell. 70: 1059-1068. doi:10.1016/0092-8674(92) ... "Construction and characterization of a human bacterial artificial chromosome library". Genomics. 34: 213-8. doi:10.1006/geno. ...
  https://en.wikipedia.org/wiki/Laurent_Susini
*  Frontiers | Induced pluripotent stem cells: applications in regenerative medicine, disease modeling, and drug discovery | Cell...
They used Human Artificial Chromosome (HAC) for the expression of complete sequence of Dystrophin (DYS). They used fibroblasts ... Further research conducted on human stem cells (HSCs) made burgeoning use of human ESCs for which embryo needed to be isolated ... 2010). Generation of functional human hepatic endoderm from human induced pluripotent stem cells. Hepatology 51, 329-335. doi: ... a newly discovered drug or therapy must be tested on human cells or human test models itself. These reasons make it more ...
  https://www.frontiersin.org/articles/10.3389/fcell.2015.00002/full
*  Bacterial artificial chromosome - Wikipedia
Cosmid End-sequence profiling Fosmid Human artificial chromosome Yeast artificial chromosome O'Connor M, Peifer M, Bender W ( ... A bacterial artificial chromosome (BAC) is a DNA construct, based on a functional fertility plasmid (or F-plasmid), used for ... The bacterial artificial chromosome's usual insert size is 150-350 kbp. A similar cloning vector called a PAC has also been ... BACs can also be utilized to detect genes or large sequences of interest and then used to map them onto the human chromosome ...
  https://en.wikipedia.org/wiki/Bacterial_artificial_chromosome
*  Lombardo A[au] - PubMed - NCBI
... enables genetic correction of human muscle progenitors and engineering of next-generation human artificial chromosomes for ... IL-10-Engineered Human CD4+ Tr1 Cells Eliminate Myeloid Leukemia in an HLA Class I-Dependent Mechanism. ... First-in-Human Evaluation of the Safety and Immunogenicity of an Intranasally Administered Replication-Competent Sendai Virus- ...
  https://www.ncbi.nlm.nih.gov/pubmed?access_num=Lombardo+A&link_type=AUTHORSEARCH&cmd=search&term=Lombardo+A%5Bau%5D&dispmax=50
*  Germinal choice technology - Wikipedia
Germline engineering and even the engineering of human artificial chromosomes (which presently allow increased reliability) are ... This had already been done in mice and, if successful in humans, could be the first case of germline engineering. Designer baby ... Chinese scientists reported in 2015 that they had modified the genomes of non-viable human embryos, Fredreik Lanner, a Swedish ... Callaway, Ewen (2016-02-04). "UK scientists gain licence to edit genes in human embryos". Nature. 530 (7588): 18-18. doi: ...
  https://en.wikipedia.org/wiki/Germinal_choice_technology
*  HAC stability in murine cells is influenced by nuclear localization and chromatin organization
Alazami AM, Mejía JE, Larin Monaco Z. Human artificial chromosomes containing chromosome 17 alphoid DNA maintain an active ... Human artificial chromosome; MMCT: Microcell mediated chromosome transfer; PFGE: Pulse field gel electrophoresis. ... Input DNA ratio determines copy number of the 33 kb Factor IX gene on de novo human artificial chromosomes. Mol Ther. 2008;16: ... Human artificial chromosomes with alpha satellite-based de novo centromeres show increased frequency of nondisjunction and ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC2674426/?lang=en-ca