The region 3′ to Xist mediates X chromosome counting and H3 Lys‐4 dimethylation within the Xist gene | The EMBO Journal
Heard E, Mongelard F, Arnaud D, Avner P (1999) Xist yeast artificial chromosome transgenes function as X‐inactivation centers ... Interestingly, comparative sequence analysis has identified a 2 kb region of mouse/human conservation (80610-82632 of Genbank ... A counting process senses the X chromosome/autosome ratio and ensures that X chromosome inactivation (XCI) initiates in the ... Rastan S, Robertson EJ, Rasmussen TP, Mastrangelo MA, Eden A, Pehrson JR, Jaenisch R (1985) X‐chromosome deletions in embryo‐ ...http://emboj.embopress.org/content/23/3/594
Case Westerners Construct First Artificial Human Chromosomes | BioWorld
BioWorld Online is the news service of record for the biotechnology industry and is updated every business morning. BioWorld Online will keep you up to date on all of the industry's business, science and regulatory news -- mergers and collaborations, FDA hearings and results, breakthroughs in research and much more.http://www.bioworld.com/content/case-westerners-construct-first-artificial-human-chromosomes
Human artificial chromosome - wikidoc
Yeast artificial chromosomes and bacterial artificial chromosomes were invented before human artificial chromosomes, which ... A human artificial chromosome (short HAC) is a microchromosome that can act as a new chromosome in a population of human cells ... They are useful in expression studies as gene transfer vectors and are a tool for elucidating human chromosome function. Grown ... Formation of de novo centromeres and construction of first-generation human artificial microchromosomes in Nature Genetics 15, ...https://www.wikidoc.org/index.php/Human_artificial_chromosome
Human artificial chromosome - Wikipedia
Yeast artificial chromosomes and bacterial artificial chromosomes were created before human artificial chromosomes, which first ... A human artificial chromosome (HAC) is a microchromosome that can act as a new chromosome in a population of human cells. That ... In 2010, a refined human artificial chromosome called 21HAC was reported. 21HAC is based on a stripped copy of human chromosome ... "A new chromosome 14-based human artificial chromosome (HAC) vector system for efficient transgene expression in human primary ...https://en.wikipedia.org/wiki/Human_artificial_chromosome
A new generation of human artificial chromosomes for functional genomics and gene therapy. - PubMed - NCBI
Generation of human artificial chromosomes via bottom-up approach. Each human chromosome contains a centromere consisting of ... An example of construction of the engineered human artificial chromosome via top-down approach. a The human chromosome 21 was ... A new generation of human artificial chromosomes for functional genomics and gene therapy.. Kouprina N1, Earnshaw WC, Masumoto ... An example of construction of the de novo generated human artificial chromosome via bottom-up approach using a synthetic ...https://www.ncbi.nlm.nih.gov/pubmed/22907415
Reversible immortalisation enables genetic correction of human muscle progenitors and engineering of next‐generation human...
Reversible immortalisation of human dystrophic muscle progenitors with a novel human artificial chromosome (HAC) containing the ... Tedesco FS (2015) Human artificial chromosomes for Duchenne muscular dystrophy and beyond: challenges and hopes. Chromosome Res ... Generation of a novel human artificial chromosome containing the entire human dystrophin locus. To facilitate pre‐clinical ... Kouprina N, Tomilin AN, Masumoto H, Earnshaw WC, Larionov V (2014) Human artificial chromosome‐based gene delivery vectors for ...http://embomolmed.embopress.org/content/early/2017/12/14/emmm.201607284?elqTrackId=980eab4fc96d4e51ad4066574838e34a
Retargeting of microcell fusion towards recipient cell-oriented transfer of human artificial chromosome | BMC Biotechnology |...
Human artificial chromosomeMeasles Virus fusogenic proteinChimeric protein. Background. Microcell-mediated chromosome transfer ... Human artificial chromosome (HAC) vector with a conditional centromere for correction of genetic deficiencies in human cells. ... Human artificial chromosome (HAC) vector provides long-term therapeutic transgene expression in normal human primary ... A gene delivery system with a human artificial chromosome vector based on migration of mesenchymal stem cells towards human ...https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12896-015-0142-z
HSV-1 delivery of artificial chromosomes to human cells - Physiology, Anatomy and Genetics
Expression of human smooth muscle calponin in transgenic mice revealed with a bacterial artificial chromosome | Heart and...
Subsequent reports using either yeast artificial chromosomes, bacterial artificial chromosomes (BACs), or P1 phage artificial ... Expression of human smooth muscle calponin in transgenic mice revealed with a bacterial artificial chromosome. Joseph M. Miano ... 1997) Human apolipoprotein B transgenic mice generated with 207- and 145-kilobase pair bacterial artificial chromosomes: ... Expression of human smooth muscle calponin in transgenic mice revealed with a bacterial artificial chromosome ...http://ajpheart.physiology.org/content/282/5/H1793.long
Genes | Free Full-Text | The Past, Present, and Future of Human Centromere Genomics
... assembly that have provided important insight into the abundance and complex organization of this sequence on human chromosomes ... Human centromeres are located at repetitive alpha satellite DNA arrays that compose approximately 5% of the genome. Contiguous ... In light of these new findings, we offer perspectives for future studies of human centromere assembly and function. ... centromere genomics spanning the discovery of the sequence to its molecular characterization and the work done during the Human ...http://www.mdpi.com/2073-4425/5/1/33
CENP-C, an autoantigen in scleroderma, is a component of the human inner kinetochore plate. - Semantic Scholar
... we used indirect immunofluorescence to demonstrate that these antibodies recognize centromeres of HeLa chromosomes in the ... These antibodies were shown to recognize a protein at a position appropriate for CENP-C on immunoblots of human chromosomal ... Second, we have demonstrated that this polypeptide shares at least two epitopes with human CENP-C. Polyclonal antibodies were ... We have isolated and characterized a set of overlapping cDNA clones that encode the human centromere autoantigen centromere ...https://www.semanticscholar.org/paper/CENP-C%2C-an-autoantigen-in-scleroderma%2C-is-a-of-the-Saitoh-Tomkiel/c0aeb56befc8ffd6bbd1f9fd2e0675b4fb420c3a
Nonviral Vector Gene Modification of Stem Cells for Myocardial Repair | Springer for Research & Development
Human artificial chromosomes. Replication-deficient recombinant viral vectors have commonly been used for gene transfer into ... 2004). Angiomyogenesis for cardiac repair using human myoblasts as carriers of human vascular endothelial growth factor. J. Mol ... Ye L, Haider H, Jiang S, Ling LH, Ge R, Law PK, Sim EK (2005). Reversal of myocardial injury using genetically modulated human ... 2006). Efficient transient genetic labeling of human CD34+ progenitor cells for in vivo application. Regen. Med. 1:223-34. ...https://rd.springer.com/article/10.2119%2F2007-00092.Haider
Forbidden flavors: scientists consider how disgusting tastes can linger surreptitiously in memory. - Free Online Library
Human artificial chromosome created.. Topics:. Animal learning. Research. Aversive stimuli. Research. Food habits ... Garcia's research also inspires psychologists now investigating central realms of human knowledge and learning, such as ... especially for humans." For his part, Garcia classifies the effect that he has championed as simply one of many "instincts for ... humans show comparable taste aversions if nauseating injections are delivered during sleep. Garcia describes this research in ...https://www.thefreelibrary.com/Forbidden+flavors%3A+scientists+consider+how+disgusting+tastes+can+...-a019308368
Table of Contents - May 08, 2001, 98 (10) | PNAS
Neocentromeres and human artificial chromosomes: An unnatural act Huntington F. Willard. PNAS May 8, 2001 98 (10) 5374-5376; ... Sequence conservation at human and mouse orthologous common fragile regions, FRA3B/FHIT and Fra14A2/Fhit Takeshi Shiraishi, ... Y chromosome polymorphism is a strong determinant of male fitness in Drosophila melanogaster Adam K. Chippindale and William R ... Involvement of a human gene related to the Drosophila spen gene in the recurrent t(1;22) translocation of acute megakaryocytic ...http://www.pnas.org/content/98/10
Bioline International Official Site (site up-dated regularly)
Advances in human artificial chromosome technology. Trends Genet 18: 313-319.. *Lauterbach H, Ried C, Epstein AL, Marconi P, ... A novel human artificial chromosome gene expression system using herpes simplex virus type 1 vectors. EMBO Rep 7: 911-918. ... Amplicons are the only viral vectors available that can deliver human or mice artificial chromosomes to the nuclear environment ... sequences and the third uses the technology of human artificial chromosomes (HACs). ...http://www.bioline.org.br/request?oc09064
US20100287636A1 - Compositions and methods for inhibiting inducible t cell kinase (itk) and treating asthma and bronchial...
... mammalian artificial chromosomes (MACs), see, e.g., U.S. Pat. Nos. 5,721,118; 6,025,155; human artificial chromosomes, see, e.g ... 15:333-335; yeast artificial chromosomes (YAC); bacterial artificial chromosomes (BAC); P1 artificial chromosomes, see, e.g., ... INTERACTION OF HUMAN BETA AMYLOID PRECURSOR PROTEIN (β-APP) WITH HUMAN LON-PROTEASE LIKE PROTEIN (HSLON) ... Human cyclic nucleotide phosphodiesterase WO2008137633A2 (en) 2008-11-13. Methods of modulating cellular homeostatic pathways ...https://patents.google.com/patent/US20100287636/en?oq=Compositions+and+methods+for+inhibiting+inducible+T+cell+Kinase+%28ITK%29+and+treating+asthma+and+bronchial+inflammations
A new assay for measuring chromosome instability (CIN) and identification of drugs that elevate CIN in cancer cells | BMC...
This quantitative assay for chromosome mis-segregation is based on the use of a non-essential human artificial chromosome (HAC ... Using the HAC-based chromosome loss assay, we have analyzed several well-known anti-mitotic, spindle-targeting compounds, all ... The identification of new compounds that increase chromosome mis-segregation rates should expedite the development of new ... this new and simple assay allows for a quick and efficient screen of hundreds of drugs to identify those affecting chromosome ...https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-13-252
Call for abstract | Synthetic Biology Conference | Computational Biology | Worldwide events | Singapore | Asia Pacific | USA |...
Track 4:Mammalian Synthetic Biology (Human Artificial Chromosomes) Scientists tend to engineer human cells to remember past ... Track 3:Artificial DNA synthesis Artificial DNA synthesis, sometimes known as DNA printing is a method in synthetic biology ... In contrast, the previous Sanger sequencing technology, used to decipher the human genome, required over a decade to deliver ... Methods in genome engineering are: Insertion involves introducing a gene into a chromosome to obtain a new function. ...https://syntheticbiology.conferenceseries.com/asiapacific/2017/call-for-abstracts.php
Department of Biomedical Engineering: People
Chromosome biology, genome stability, gene expression, human artificial chromosome. Research Associate Professor. *Alessandra ... Chromosome biology, cytogenetics, human artificial chromosome. *Angelo Sassaroli, Ph.D., University of Electro-Communications, ... Tissue Models to study human disease processes in stratified squamous epithelium. *Jim Harden, Adjunct, Ph.D., University of ... Novel methods for the rational design and construction of artificial proteins, molecular enzymes and self-assembling ...https://engineering.tufts.edu/bme/people/
Simple and efficient vectors for retrofitting BACs and PACs with mammalian neoR and EGFP marker genes. - Semantic Scholar
... and P1 artificial chromosomes (PACs) are widely used to investigate the functions of genes and genomes in mammalian cells in ... Rapid creation of BAC-based human artifical chromosome vectors by transposition with synthetic alpha-satellite arrays. *Joydeep ... Bacterial artificial chromosomes (BACs) and P1 artificial chromosomes (PACs) are widely used to investigate the functions of ... Prospects for the Use of Artificial Chromosomes and Minichromosome-Like Episomes in Gene Therapy. *Sara Pérez-Luz, Javier Díaz- ...https://www.semanticscholar.org/paper/Simple-and-efficient-vectors-for-retrofitting-BACs-Kaname-Huxley/51af713567ddf7384d38c1dea23f73e66acf3a39
Bacterial Artificial Chromosome Mutagenesis Using Recombineering
kb-alphoid DNA into a human BAC clones via the λ Red recombineering system in an attempt to build human artificial chromosomes ... Construction of human artificial chromosome vectors by recombineering. Gene. 2005;351:29-38. [PubMed] ... Efficient and precise engineering of a 200 kb β-globin human/bacterial artificial chromosome in E. coli DH10B using an ... Human apolipoprotein B transgenic mice generated with 207- and 145- kilobase pair bacterial artificial chromosomes. Evidence ...http://pubmedcentralcanada.ca/pmcc/articles/PMC3005948/
Genetics, Gene Therapy & Genetic Disease | EMBO Molecular Medicine
Reversible immortalisation of human dystrophic muscle progenitors with a novel human artificial chromosome (HAC) containing the ... Reversible immortalisation of human dystrophic muscle progenitors with a novel human artificial chromosome (HAC) containing the ... generation human artificial chromosomes for Duchenne muscular dystrophy ... 4Chromosome Engineering Research Center (CERC), Tottori University, Yonago, Tottori, Japan. *. 5Division of Neuroscience, ...http://embomolmed.embopress.org/collection/genetics-gene-therapy-genetic-disease?page=2
Chromosome Centromeres Are Inherited Epigenetically - Redorbit
This discovery may help to further the development of artificial human chromosomes, which could be used for gene therapies in ... Scientists would like to develop artificial human chromosomes as an alternative to gene therapy using viruses. "Like their ... During cell division, the kinetochore provides a point of attachment for the cell skeleton and enables the chromosomes to move ... The cell skeleton, which distributes the chromosomes to the two daughter cells during cell division, attaches to the ...http://www.redorbit.com/news/science/1112415890/chromosome-centromeres-are-inherited-epigenetically/
Frontiers | Advancements in stem cells treatment of skeletal muscle wasting | Physiology
Taking into account the advances in the isolation of new subpopulation of stem cells and in the creation of artificial stem ... Taking into account the advances in the isolation of new subpopulation of stem cells and in the creation of artificial stem ... 2011). Stem cell-mediated transfer of a human artificial chromosome ameliorates muscular dystrophy. Sci. Transl. Med. 3, 96ra78 ... iPSCs and human disease. Since the work of Yamanaka was published, reprogramming of cells provided a realistic way not only to ...https://www.frontiersin.org/articles/10.3389/fphys.2014.00048/full
Centromeric Localization and Adaptive Evolution of an Arabidopsis Histone H3 Variant | Plant Cell
Willard, H.F. (1998). Centromeres: The missing link in the development of human artificial chromosomes. Curr. Opin. Genet. Dev. ... In pachytene chromosomes, we found weak signal over the euchromatic arms of the chromosomes, but not over heterochromatic ... Haaf, T., and Willard, H.F. (1997). Chromosome-specific alpha-satellite DNA from the centromere of chimpanzee chromosome 4. ... and spots could be seen to lie centrally on chromosomes (Figure 3B). The spots were on the leading portions of the chromosomes ...http://www.plantcell.org/content/14/5/1053.long