B: Second DEAE-cellulose column chromatography. After loading, the 5-ml column was washed with 30 ml of 25 mmol/l Tris-acetate ... A: First DEAE-cellulose column chromatography. After sample loading, the 15-ml column was washed with 75 ml of binding buffer. ... The supernatant was passed through 0.4-μm filters and then chromatographed on DEAE-cellulose (DE-52, Whatman, 15-ml bed) ... and again chromatographed on DEAE-cellulose (5-ml column). KHK was eluted with a linear gradient of 25-200 mmol/l Tris-acetate ...http://diabetes.diabetesjournals.org/content/52/9/2426
Purification, identification, and cloning of lysoplasmalogenase, the enzyme that catalyzes hydrolysis of the vinyl ether bond...
A, anion exchange chromatography on DEAE-cellulose open column. B, hydroxyapatite chromatography CHT5 column, FPLC. C, gel ... DEAE-cellulose (5 nmol/min); lane 5, hydroxyapatite (41 nmol/min); lane 6, gel filtration (82 nmol/min); lane 7, Mono Q ... D, anion exchange chromatography on Mono Q 5/50 GL column, FPLC. Fractions were tested for lysoplasmalogenase activity (black ... experiment in which microsomes from seven rat livers were solubilized and purified using these four column chromatography steps ...https://www.ncbi.nlm.nih.gov/pubmed/21515882
CiNii 論文 - What have we learned about sea urchin sperm bindin?
Purification of sea urchin sperm bindin by DEAE-cellulose chromatography VACQUIER V. D. ...http://ci.nii.ac.jp/naid/10008754405
Brevet EP0329822B1 - Nucleic acid amplification process - Google Brevets
Oligonucleotides were purified by polyacrylamide gel electrophoresis followed by DEAE cellulose chromatography. The ...http://www.google.fr/patents/EP0329822B1?cl=en
CHROMATOGRAPHY. Hb X was isolated by DEAE-cellulose chromatography. STRUCTURE STUDIES. The tryptic peptides were separated by ... Hb X moves slower than Hb S at alkaline pH (cellulose acetate). ...http://globin.cse.psu.edu/globin/html/huisman/variants/alpha/Cordele.html
CHROMATOGRAPHY. Hb X was isolated by DEAE-cellulose chromatography. STRUCTURE STUDIES. Tryptic digestion; fingerprinting; amino ...http://globin.cse.psu.edu/html/huisman/variants/beta/Riverdale-Bronx.html
CHROMATOGRAPHY. No separations reported except by DEAE-cellulose chromatography. STRUCTURE STUDIES. Tryptic digestion of betaX ...http://globin.cse.psu.edu/html/huisman/variants/beta/Mozhaisk.html
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
by DEAE cellulose chromatography and Sephacryl S-400 size-exclusion chromatography. The structural characterisation, ... The levels of MUC5AC protein and ATP in culture medium were detected by ELISA and high performance liquid chromatography, ...https://www.jove.com/visualize?author=Ting-Feng%20Wu%20
US Patent # 4,839,293. DNA encoding streptavidin, streptavidin produced therefrom, fused polypeptides which include amino...
Unincorporated ATP was removed by chromatography on DEAE-cellulose (13). Construction of the genomic library from Streptomyces ...http://patents.com/us-4839293.html
Enzymatic Synthesis of Acetono-CoA, A Competitive Inhibitor of Acetyl-CoA with Citrate Synthase | SpringerLink
Products and reactants were isolated by DEAE-cellulose column chromatography and peaks were identified by TLC. Enzymatic ...https://link.springer.com/chapter/10.1007/978-1-4684-8640-7_46
Patent US5643722 - Methods for the detection and isolation of proteins - Google Patents
... phenol extraction followed by chromatography on DEAE-cellulose. Amino-acid specific tRNA, for example tRNAfMet, can be isolated ... reverse-phase chromatography, affinity chromatography and ion exchange chromatography. These techniques are very useful for ... The resulting mixture was applied to a DEAE-cellulose column (1 ml), equilibrated with 50 mM NaOAc, pH 4.5, 10 mM MgCl2, at 4 C ... high-pressure or fast-pressure liquid chromatography, affinity chromatography, ion exchange chromatography, chemical extraction ...http://www.google.ca/patents/US5643722
The antibody was dialyzed against phosphate buffer (pH 8.0) and purified by DEAE-cellulose chromatography. ... HbA1c was measured by an HPLC cation-exchange chromatography system (HA-8,121; Kyoto Daiichi, Kyoto, Japan). The intra-assay ... Cai J, Hurst HE: Identification and quantitation of N-(carboxymethyl)valine adduct in hemoglobin by gas chromatography/mass ... CMV-Hb-in circulating erythrocytes by gas chromatography. Our results for the CMV-Hb value of healthy subjects were similar to ...http://care.diabetesjournals.org/content/24/5/891
Ankyrin-Tiam1 Interaction Promotes Rac1 Signaling and Metastatic Breast Tumor Cell Invasion and Migration | JCB
The anti-Tiam1 IgG fraction was prepared by conventional DEAE-cellulose chromatography. Mouse monoclonal anti-hemagglutinin (HA ... B) A Coomassie blue stain of the 116-kD GST-ARD fusion protein purified by affinity column chromatography (lane 1), and the 89- ... obtained from anti-Tiam1 affinity column chromatography) in the absence or the presence of 100-fold excess amount of unlabeled ... and GFP-tagged Tiam1 fragment fusion purified by anti-GFP-conjugated affinity column chromatography (lane 2). (C, a) Binding of ...http://jcb.rupress.org/content/150/1/177
Gene switching in Amoeba proteus caused by endosymbiotic bacteria | Journal of Cell Science
A) Results of Sephadex G-150 chromatography of D and xD amoebae. (B) Results of DEAE-cellulose chromatography of D and xD ... Results of western blot analyses of proteins fractionated by Sephadex G-150 chromatography. Fractions containing SAMS enzyme ...http://jcs.biologists.org/content/117/4/535.figures-only
Schistosoma Mansoni Soluble Egg Antigens | The Journal of Immunology
The eluted antigens were separated from each other by ion exchange chromatography on DEAE cellulose. On polyacrylamide gel ...http://www.jimmunol.org/content/117/5_Part_1/1553
US20100323430A1 - Media For Membrane Ion Exchange Chromatography - Google Patents
Separation of adenovirus by chromatography on DEAE-cellulose. Virology 1961, 13, (2), 264). Membrane chromatography has started ... Chromatography has been used for virus purification, primarily in the format of beads. First reports on chromatography-based ... B01D15/08-Selective adsorption, e.g. chromatography * B01D15/26-Selective adsorption, e.g. chromatography characterised by the ... Chromatography column US4861705A (en) * 1983-01-31. 1989-08-29. Yeda Research And Development Company, Ltd.. Method for ...https://patents.google.com/patent/US20100323430
Hb X elutes between Hb A2 and Hb A on DEAE-cellulose chromatography; the betaX chain separates from the betaA and alpha chains ... CHROMATOGRAPHY. Hb X moves like Hb E in cation exchange HPLC; does not separate from Hb A2; ...http://globin.cse.psu.edu/html/huisman/variants/beta/Sunnybrook.html
Frontiers | Effect of the Camelid's Seminal Plasma Ovulation-Inducing Factor/β-NGF: A Kisspeptin Target Hypothesis | Veterinary...
... by DEAE-cellulose chromatography. Reprod Dom Anim (2001) 36(3-4):177-81. doi:10.1046/j.1439-0531.2001.d01-33.x ... The 19.431 kDa proteic camel extract was eluted along a DEAE-cellulose column into seven fractions. Of all fractions injected ... isolated by hydroxylapatite column chromatography), fraction C2 (isolated by gel filtration chromatography), or phosphate- ...https://www.frontiersin.org/articles/10.3389/fvets.2017.00099/full
CHROMATOGRAPHY. Hb X and Hb A can be separated by DEAE-cellulose or DEAE-Sephadex chromatography. ... Tryptic digestion of AE-betaX chain; separation of peptides by fingerprinting or cation exchange chromatography; amino acid ...http://globin.cse.psu.edu/globin/html/huisman/variants/multi-beta/C-Ziquinchor.html
Medline ® Abstract for Reference 14 of 'Classification and causes of jaundice or asymptomatic...
... activity for bilirubin and 4-nitrophenol was separated from solubilized rat liver microsomes by DEAE-cellulose chromatography ... enzyme activities were reflected in the enzymatically active fractions separated after DEAE-cellulose chromatography. The ...http://www.uptodate.com/contents/classification-and-causes-of-jaundice-or-asymptomatic-hyperbilirubinemia/abstract/14
Hypersensitivity to Ascaris Antigens | The Journal of Immunology
The crude extract was fractionated by DEAE-cellulose chromatography and the fractions were tested in P-K type reactions. The ...http://www.jimmunol.org/content/101/4/628
IJBT JUL 2009
... followed by DEAE-cellulose column chromatography. The enzyme was optimally active at 55oC and pH 9.0. Influence of metal ions ... The crude extract subjected to gel filtration chromatography yielded 60 fractions; the fractions 7, 12 and 55 showed ...http://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbt/ijbt2k9/ijbt_jul09.asp
The two enzyme activities were separable by ion-exchange chromatography on DEAE-cellulose and by gel filtration on Sephacryl. ...http://cancerres.aacrjournals.org/content/41/11_Part_1/4346
Molecules | Free Full-Text | High Molecular Weight Glucan of the Culinary Medicinal Mushroom Agaricus bisporus is an α-Glucan...
... ethanol precipitation and DEAE-cellulose chromatography. The resulting material showed a single HMW peak excluded from a ... ethanol precipitation and DEAE-cellulose chromatography. The resulting material showed a single HMW peak excluded from a ...http://www.mdpi.com/1420-3049/15/8/5818
Studies on the translocation inhibitor of tritium labeled dexamethasone receptor complex - Request PDF
The liver inhibitor was macromolecular and clearly separated from the DEX-receptor complex on DEAE-cellulose chromatography. ...https://eurekamag.com/research/006/522/006522623.php
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