Synthetic heparan sulfate dodecasaccharides reveal single sulfation site interconverts CXCL8 and CXCL12 chemokine biology |...
Cell Migration Assays, Leukocyte, Chemokine CXCL12, Heparitin Sulfate, Human Umbilical Vein Endothelial Cells, Humans, ...https://www.research.manchester.ac.uk/portal/en/publications/synthetic-heparan-sulfate-dodecasaccharides-reveal-single-sulfation-site-interconverts-cxcl8-and-cxcl12-chemokine-biology
CORM-3-derived CO modulates polymorphonuclear leukocyte migration across the vascular endothelium by reducing levels of cell...
... and total/differential cell counts (A) and protein concentrations (Bradford assay; B) were assessed. Results are means ± SE of ... CORM-3-derived CO modulates polymorphonuclear leukocyte migration across the vascular endothelium by reducing levels of cell ... CORM-3-derived CO modulates polymorphonuclear leukocyte migration across the vascular endothelium by reducing levels of cell ... CORM-3-derived CO modulates polymorphonuclear leukocyte migration across the vascular endothelium by reducing levels of cell ...http://ajpheart.physiology.org/content/297/3/H920
Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay | Protocol
... trunk neural crest cells) is described. This method is inexpensive, gentle,... ... An approach to analyze the migration of explanted cells ( ... Zigmond, S. H. Ability of polymorphonuclear leukocytes to ... Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay. Christopher C. Walheim1, Juan Pablo Zanin ... An approach to analyze the migration of explanted cells (trunk neural crest cells) is described. This method is inexpensive, ...https://www.jove.com/video/3330/analysis-trunk-neural-crest-cell-migration-using-modified-zigmond
Label-free, Live Cell Imaging of Transendothelial Migration | The Journal of Immunology
... we evaluated the ability of this novel assay to quantify leukocyte transmigration in the absence of cell labeling. Briefly, ... Label-free, Live Cell Imaging of Transendothelial Migration Message Subject (Your Name) has forwarded a page to you from The ... Label-free, Live Cell Imaging of Transendothelial Migration. Belinda OClair, Guangnan Li and Daniel Appledorn ... The recruitment of leukocytes to sites of infection and their migration through the endothelium are critical to immune ...http://www.jimmunol.org/content/196/1_Supplement/119.22
A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration | Protocol
... we present a protocol to evaluate the effect of peptides on the migration of bronchial epithelial cells. This method allows for ... polymorphonuclear leukocytes). ... to stimulate cell migration. Importantly, cell migration is a ... Indeed, cell migration is a rate-limiting event in wound healing, and studying factors that can affect cell migration will help ... The cells at the edge of the artificial 'wounded' field will start migrating into the cell-free area, forming new cell-cell ...https://www.jove.com/video/56825/a-novel-in-vitro-wound-healing-assay-to-evaluate-cell-migration
Selection Guide for Boyden Chamber Cell Migration and Invasion Assays | Cell Biolabs, Inc.
Solutions including novel assays and reagents to advance cell and molecular biology. ... Migration of cells toward a chemoattractant (chemical signal) in the cell's surrounding environment. Leukocytes. Neutrophils. 3 ... Selection Guide for Boyden Chamber Cell Migration and Invasion Assays. Assay Type. Definition. Cell Types. Pore Size. Insert ... Slow-moving Cells. 12 µm. None. 24-Well. Haptotaxis. Migration of cells along a gradient of cellular adhesion sites or ...https://www.cellbiolabs.com/selection-guide-cell-migration-and-invasion-assays
Collagen Type I, Rat Tail | For 3D Cell Culture Gels | ibidi
A high-quality collagen solution providing in vivo-like ECM structures for 3D cell culture , High concentration , Storage at - ... Chemotaxis assays in firm 3D collagen gels. *Leukocyte and tumor cell migration ... Chemotaxis of HT-1080 cells, which are migrating towards FCS, using low resolution microscopy for a maximum of migration data ... High resolution microscopy during chemotaxis and cell polarization, using the HT-1080 LifeAct cell line ...https://ibidi.com/cell-culturemicroscopy-reagents/107-collagen-type-i-rat-tail.html
Irradiation Induces Upregulation of CD31 in Human Endothelial Cells | Arteriosclerosis, Thrombosis, and Vascular Biology
Transendothelial Migration Assay. The transendothelial migration of leukocytes was quantified in irradiated and nonirradiated ... Cell Adherence Studies. U-937 cells were maintained at cell concentrations between 2×105 cells/mL and 9×105 cells/mL in RPMI ... Platelet endothelial cell adhesion molecule, PECAM-1 modulates cell migration. J Cell Physiol. 1992;153:417-428. ... The Proportion of Cells in Each Phase of the Cell Cycle. The proportion of cells in various phases of the cell cycle was ...http://atvb.ahajournals.org/content/19/3/588
CEACAM1 carcinoembryonic antigen related cell adhesion molecule 1 [Homo sapiens (human)] - Gene - NCBI
leukocyte migration TAS Traceable Author Statement. more info. negative regulation of T cell mediated cytotoxicity IDA Inferred ... colocalizes_with T cell receptor complex IDA Inferred from Direct Assay. more info ... Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded ... CEACAM1 overexpression significantly suppressed MM cell proliferation, induced cell apoptosis, and inhibited cell invasion and ...https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=ShowDetailView&TermToSearch=634&ordinalpos=1&itool=EntrezSystem2.PEntrez.Gene.Gene_ResultsPanel.Gene_RVDocSum
Toxins | Free Full-Text | Effects of Clostridium difficile Toxin A and B on Human T Lymphocyte Migration | HTML
... direct and indirect effects of TcdA and TcdB on the migration of human blood T cells using conventional cell migration assays ... The effects of C. difficile toxins on the migration and trafficking of other leukocyte subsets, such as T lymphocytes, are not ... Similar effects are observed in T cell migration toward the TcdA- or TcdB-treated human epithelial cells. Our study ... It has been found that, although both toxins decrease T cell motility, only TcdA but not TcdB decreases T cell chemotaxis. ...http://www.mdpi.com/2072-6651/5/5/926/htm
Mouse Aorta Smooth Muscle Cells Differentiate Into Lymphoid Tissue Organizer-Like Cells on Combined Tumor Necrosis Factor...
... supernatants of SMC were examined in a migration assay using naive splenocytes from young C57BL/6J mice as targets. There was ... we next examined the leukocyte lineage composition of migrated cells by FACS using CD3 for total T cells, CD19 for B cells, and ... However, evidence that macrophage/foam cells/dendritic-like cells (DC)7 and T cells may mediate antigen-dependent T-cell ... Activated SMC Promote Migration of Naive Splenic T Cells, B Cells, and Macrophages/DC Through Soluble Chemotactic Molecules. To ...http://atvb.ahajournals.org/content/30/3/395
Foxp3+-Inducible Regulatory T Cells Suppress Endothelial Activation and Leukocyte Recruitment | The Journal of Immunology
In vitro assay for leukocyte adhesion and transendothelial migration. T cell adhesion to EC monolayers and transendothelial ... 107 cells/ml, and small boluses (3 × 106 of each cell type) of a mixture of both cells were injected retrograde into the ... mouse heart endothelial cell. nTreg. naturally occurring regulatory T cells. RA. retinoic acid. sTNFRII. soluble TNFRII. Treg. ... Foxp3+-Inducible Regulatory T Cells Suppress Endothelial Activation and Leukocyte Recruitment. Elena Maganto-García, De-xiu Bu ...http://www.jimmunol.org/content/187/7/3521.long
Transplantation of Human Pericyte Progenitor Cells Improves the Repair of Infarcted Heart Through Activation of an Angiogenic...
473 pg/mL/105 cells, n=6), SVP-CM stimulated by 2.0±0.3-fold the migration of human CD14+CD16+ cells in a transwell assay (P, ... and C and are negative for class II human leukocyte antigen HLA-DR. SVPs were also negative for CD80 and Fas ligand as assessed ... mononuclear cells. MSC. mesenchymal stem cells. MSC-CM. conditioned medium from mesenchymal stem cells. PC. progenitor cells. ... Accordingly, the SVP-CM increases the migration of human CD14+CD16+ cells in a transwell assay. ...http://circres.ahajournals.org/content/109/8/894
CD2 - T-cell surface antigen CD2 precursor - Homo sapiens (Human) - CD2 gene & protein
... and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the ... leukocyte migration Source: Reactome. *membrane raft polarization Source: UniProtKBTraceable author statementi*11376005 ... heterotypic cell-cell adhesion Source: UniProtKB ,p>Inferred from Direct Assay,/p> ,p>Used to indicate a direct assay for the ... and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the ...https://www.uniprot.org/uniprot/P06729
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
We conclude that the 3D collagen matrix migration assay is a versatile method to analyze the migration of cells within a ... Various cell types could be analyzed using this technique, including lymphocytes/leukocytes, stem cells, and tumor cells. ... Within the past years various cell migration assays have been developed to analyze the migratory behavior of different cell ... We found that some tumor cell lines such as melanoma B16F1 cells, glioblastoma U87 cells, and breast cancer MDA-MB-435 cells ...https://www.jove.com/visualize/abstract/19584911/aqp1-is-not-only-water-channel-it-contributes-to-cell-migration
Autoimmune T-Cell Reactivity to Myelin Proteolipids and Glycolipids in Multiple Sclerosis
Using the leukocyte migration inhibition assay, Beraud et al.  also showed that T cells from MS patients undergoing an ... "T cell recognition of self-antigen presenting cells by protein transfer assay reveals a high frequency of anti-myelin T cells ... and the T-cell recognition of antigen presenting cells by protein transfer (TRAP) assay , which has recently been shown to ... 2.1.2. T-Cell Responses to PLP Peptides. Because of the difficulties encountered when using whole PLP in immune assays, most ...https://www.hindawi.com/journals/msi/2013/151427/
Interaction between Tumor Cell Surface Receptor RAGE and Proteinase 3 Mediates Prostate Cancer Metastasis to Bone | Cancer...
Migration of PC3, PC3/Fl-RAGE, PC3/Nt-RAGE, and DU145 cells was evaluated in a transwell assay in the presence of PR3 and JNK ... PR3 activity and cell motility assays. PR3 from primary human leukocytes (Sigma) was used unless otherwise specified. For ... Cell adhesion assays. Binding of PR3-expressing U937 cells to PC3 cells was assayed by coculturing adherent PC3 cells with a 10 ... and DU145 cells, but not PC3/Nt-RAGE cells. Cell migration was inhibited in the presence of a JNK-specific inhibitor (Fig. 3C ...http://cancerres.aacrjournals.org/content/77/12/3144
JCI - Factor XII and uPAR upregulate neutrophil functions to influence wound healing
WT BM transplanted into KO hosts corrected the leukocyte migration defect on the TG assay (Figure 10A). Alternatively, KO BM ... To further determine whether FXII promoted directional cell migration, a kinetic chemotaxis assay was developed, employing a ... FXII influences leukocyte migration into skin wounds. (A and C) Frozen sections of day 2 (D2) and day 5 (D5) wounds were ... Leukocyte migration in TG-induced peritonitis. WT and F12-/- mice were injected intraperitoneally with TG solution. At 4 hours ...https://www.jci.org/articles/view/92880
Physio-pharmacological Investigations About the Anti-inflammatory and Antinociceptive Efficacy of (+)-Limonene Epoxide |...
Pro-inflammatory cytokines and cell migration of peritoneal cells were also assessed. Antinociceptive effects were evaluated by ... An in vivo assay to test blood vessel permeability. Journal of Visualized Experiments 16, e50062.Google Scholar ... It also reduced leukocytes, neutrophils, and IL-1β levels in the peritoneal cavity in comparison with carrageenan group (P , ... semisynthetic compound cell migration inhibition cytokine reduction analgesia opioid system involvement Rivelilson Mendes de ...https://link.springer.com/article/10.1007/s10753-016-0496-y
Buy New & Used Books Online with Free Shipping | Better World Books
cell-cell adhesion, neutrophil chemotaxis, in vitro assays used to. study leukocyte migration through monolayers of cultured ... cell-cell adhesion, neutrophil chemotaxis, in vitro assays used to. study leukocyte migration through monolayers of cultured ... cells, and novel techniques to purify pseudopodia from migratory cells. This book also discusses the study of cell-matrix ... cells, and novel techniques to purify pseudopodia from migratory cells. This book also discusses the study of cell-matrix ...https://www.betterworldbooks.com/product/detail/adhesion-protein-protocols-1588295338
Immunology sub-cluster 18
Leukocyte migration inhibition factor (LMIF) assays have been used as a measure of cell-mediated immune responses. Direct ... leukocyte migration inhibitory factor (LIF). Mononuclear cells as well as purified T-cells did not elaborate LIF in the ... The migration is determined with polyclonal anti-macrophage antibodies in an ELISA assay. The numbers of macrophages thus ... Macrophage migration inhibition factor (MIF) derived from human lymphoid cell lines was found to lose biologic activity on ...http://www.biomedsearch.com/cluster/42/Immunology/sub-18-p15.html
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
We conclude that the 3D collagen matrix migration assay is a versatile method to analyze the migration of cells within a ... Various cell types could be analyzed using this technique, including lymphocytes/leukocytes, stem cells, and tumor cells. ... Within the past years various cell migration assays have been developed to analyze the migratory behavior of different cell ... We exogenously expressed a G3 construct in 66c14 cells and analyzed its effects on cell proliferation, migration, cell cycle ...https://www.jove.com/visualize/abstract/21079779/versican-g3-promotes-mouse-mammary-tumor-cell-growth-migration
Inhibitory Effect of Helicteres gardneriana Ethanol Extract on Acute Inflammation
... induced by intrapleural injection of carrageenan and the migration of polymorphonuclear cells into the inflamed pleural cavity ... rolling and adhesion of leukocytes and the number of leukocytes that migrated toward the perivascular space in response to the ... The anti-inflammatory effect of an ethanol extract of Helicteres gardneriana (Nees) Castiglioni was assayed in experimental ... the anti-inflammatory effect of the ethanol extract of Helicteres gardneriana and imply that inhibition of leukocyte- ...https://www.hindawi.com/journals/ecam/2012/141947/abs/
Chemotaxis Assays for Eukaryotic Cells - Current Protocols
Chemotaxis is a complex response of a cell to an external stimulus ... Chemoattractant receptor crosstalk as a regulatory mechanism in leukocyte adhesion and migration. Eur. J. Immunol. 27:2571‐2578 ... J. Cell Physiol. 135:355‐366. Jungi, T.W. 1975. Assay of chemotaxis by a reversible Boyden chamber eliminating cell detachment ... Measurement of the chemotaxis coefficient for human neutrophils in the under‐agarose migration assay. Cell Motil. Cytoskeleton ...http://www.currentprotocols.com/WileyCDA/CPUnit/refId-cb1201.html
More articles from Cell Migration and Adhesion | The Journal of Immunology
Development of a high throughput cell migration assay using the SRU BIND reader (102.13) Kathryn McAllister, Marla Abodeely, ... The VCAM-1 cytoplasmic domain is essential for endothelial cell signaling and leukocyte trafficking. (102.17) Michelle Marchese ... S100A4 is dispensable for T cell development and steady state lymph node entry and migration (102.15) Yi-Chun Chen and Matthew ... Loss of T cell CD98hc specifically ablates T cell clonal expansion and protects from autoimmunity. (102.16) Joseph Cantor, ...http://www.jimmunol.org/content/by/section/Cell%20Migration%20and%20Adhesion