*  CORM-3-derived CO modulates polymorphonuclear leukocyte migration across the vascular endothelium by reducing levels of cell...
... and total/differential cell counts (A) and protein concentrations (Bradford assay; B) were assessed. Results are means ± SE of ... CORM-3-derived CO modulates polymorphonuclear leukocyte migration across the vascular endothelium by reducing levels of cell ... CORM-3-derived CO modulates polymorphonuclear leukocyte migration across the vascular endothelium by reducing levels of cell ... CORM-3-derived CO modulates polymorphonuclear leukocyte migration across the vascular endothelium by reducing levels of cell ...
  http://ajpheart.physiology.org/content/297/3/H920
*  Label-free, Live Cell Imaging of Transendothelial Migration | The Journal of Immunology
... we evaluated the ability of this novel assay to quantify leukocyte transmigration in the absence of cell labeling. Briefly, ... Label-free, Live Cell Imaging of Transendothelial Migration Message Subject (Your Name) has forwarded a page to you from The ... Label-free, Live Cell Imaging of Transendothelial Migration. Belinda OClair, Guangnan Li and Daniel Appledorn ... The recruitment of leukocytes to sites of infection and their migration through the endothelium are critical to immune ...
  http://www.jimmunol.org/content/196/1_Supplement/119.22
*  Selection Guide for Boyden Chamber Cell Migration and Invasion Assays | Cell Biolabs, Inc.
Solutions including novel assays and reagents to advance cell and molecular biology. ... Migration of cells toward a chemoattractant (chemical signal) in the cell's surrounding environment. Leukocytes. Neutrophils. 3 ... Selection Guide for Boyden Chamber Cell Migration and Invasion Assays. Assay Type. Definition. Cell Types. Pore Size. Insert ... Slow-moving Cells. 12 µm. None. 24-Well. Haptotaxis. Migration of cells along a gradient of cellular adhesion sites or ...
  https://www.cellbiolabs.com/selection-guide-cell-migration-and-invasion-assays
*  Irradiation Induces Upregulation of CD31 in Human Endothelial Cells | Arteriosclerosis, Thrombosis, and Vascular Biology
Transendothelial Migration Assay. The transendothelial migration of leukocytes was quantified in irradiated and nonirradiated ... Cell Adherence Studies. U-937 cells were maintained at cell concentrations between 2×105 cells/mL and 9×105 cells/mL in RPMI ... Platelet endothelial cell adhesion molecule, PECAM-1 modulates cell migration. J Cell Physiol. 1992;153:417-428. ... The Proportion of Cells in Each Phase of the Cell Cycle. The proportion of cells in various phases of the cell cycle was ...
  http://atvb.ahajournals.org/content/19/3/588
*  Toxins | Free Full-Text | Effects of Clostridium difficile Toxin A and B on Human T Lymphocyte Migration | HTML
... direct and indirect effects of TcdA and TcdB on the migration of human blood T cells using conventional cell migration assays ... The effects of C. difficile toxins on the migration and trafficking of other leukocyte subsets, such as T lymphocytes, are not ... Similar effects are observed in T cell migration toward the TcdA- or TcdB-treated human epithelial cells. Our study ... It has been found that, although both toxins decrease T cell motility, only TcdA but not TcdB decreases T cell chemotaxis. ...
  http://www.mdpi.com/2072-6651/5/5/926/htm
*  Mouse Aorta Smooth Muscle Cells Differentiate Into Lymphoid Tissue Organizer-Like Cells on Combined Tumor Necrosis Factor...
... supernatants of SMC were examined in a migration assay using naive splenocytes from young C57BL/6J mice as targets. There was ... we next examined the leukocyte lineage composition of migrated cells by FACS using CD3 for total T cells, CD19 for B cells, and ... However, evidence that macrophage/foam cells/dendritic-like cells (DC)7 and T cells may mediate antigen-dependent T-cell ... Activated SMC Promote Migration of Naive Splenic T Cells, B Cells, and Macrophages/DC Through Soluble Chemotactic Molecules. To ...
  http://atvb.ahajournals.org/content/30/3/395
*  Foxp3+-Inducible Regulatory T Cells Suppress Endothelial Activation and Leukocyte Recruitment | The Journal of Immunology
In vitro assay for leukocyte adhesion and transendothelial migration. T cell adhesion to EC monolayers and transendothelial ... 107 cells/ml, and small boluses (3 × 106 of each cell type) of a mixture of both cells were injected retrograde into the ... mouse heart endothelial cell. nTreg. naturally occurring regulatory T cells. RA. retinoic acid. sTNFRII. soluble TNFRII. Treg. ... Foxp3+-Inducible Regulatory T Cells Suppress Endothelial Activation and Leukocyte Recruitment. Elena Maganto-García, De-xiu Bu ...
  http://www.jimmunol.org/content/187/7/3521.long
*  Transplantation of Human Pericyte Progenitor Cells Improves the Repair of Infarcted Heart Through Activation of an Angiogenic...
473 pg/mL/105 cells, n=6), SVP-CM stimulated by 2.0±0.3-fold the migration of human CD14+CD16+ cells in a transwell assay (P, ... and C and are negative for class II human leukocyte antigen HLA-DR. SVPs were also negative for CD80 and Fas ligand as assessed ... mononuclear cells. MSC. mesenchymal stem cells. MSC-CM. conditioned medium from mesenchymal stem cells. PC. progenitor cells. ... Accordingly, the SVP-CM increases the migration of human CD14+CD16+ cells in a transwell assay. ...
  http://circres.ahajournals.org/content/109/8/894
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
We conclude that the 3D collagen matrix migration assay is a versatile method to analyze the migration of cells within a ... Various cell types could be analyzed using this technique, including lymphocytes/leukocytes, stem cells, and tumor cells. ... Within the past years various cell migration assays have been developed to analyze the migratory behavior of different cell ... We found that some tumor cell lines such as melanoma B16F1 cells, glioblastoma U87 cells, and breast cancer MDA-MB-435 cells ...
  https://www.jove.com/visualize/abstract/19584911/aqp1-is-not-only-water-channel-it-contributes-to-cell-migration
*  Interaction between Tumor Cell Surface Receptor RAGE and Proteinase 3 Mediates Prostate Cancer Metastasis to Bone | Cancer...
Migration of PC3, PC3/Fl-RAGE, PC3/Nt-RAGE, and DU145 cells was evaluated in a transwell assay in the presence of PR3 and JNK ... PR3 activity and cell motility assays. PR3 from primary human leukocytes (Sigma) was used unless otherwise specified. For ... Cell adhesion assays. Binding of PR3-expressing U937 cells to PC3 cells was assayed by coculturing adherent PC3 cells with a 10 ... and DU145 cells, but not PC3/Nt-RAGE cells. Cell migration was inhibited in the presence of a JNK-specific inhibitor (Fig. 3C ...
  http://cancerres.aacrjournals.org/content/77/12/3144
*  Physio-pharmacological Investigations About the Anti-inflammatory and Antinociceptive Efficacy of (+)-Limonene Epoxide |...
Pro-inflammatory cytokines and cell migration of peritoneal cells were also assessed. Antinociceptive effects were evaluated by ... An in vivo assay to test blood vessel permeability. Journal of Visualized Experiments 16, e50062.Google Scholar ... It also reduced leukocytes, neutrophils, and IL-1β levels in the peritoneal cavity in comparison with carrageenan group (P , ... semisynthetic compound cell migration inhibition cytokine reduction analgesia opioid system involvement Rivelilson Mendes de ...
  https://link.springer.com/article/10.1007/s10753-016-0496-y
*  Buy New & Used Books Online with Free Shipping | Better World Books
cell-cell adhesion, neutrophil chemotaxis, in vitro assays used to. study leukocyte migration through monolayers of cultured ... cell-cell adhesion, neutrophil chemotaxis, in vitro assays used to. study leukocyte migration through monolayers of cultured ... cells, and novel techniques to purify pseudopodia from migratory cells. This book also discusses the study of cell-matrix ... cells, and novel techniques to purify pseudopodia from migratory cells. This book also discusses the study of cell-matrix ...
  https://www.betterworldbooks.com/product/detail/adhesion-protein-protocols-1588295338
*  Immunology sub-cluster 18
Leukocyte migration inhibition factor (LMIF) assays have been used as a measure of cell-mediated immune responses. Direct ... leukocyte migration inhibitory factor (LIF). Mononuclear cells as well as purified T-cells did not elaborate LIF in the ... The migration is determined with polyclonal anti-macrophage antibodies in an ELISA assay. The numbers of macrophages thus ... Macrophage migration inhibition factor (MIF) derived from human lymphoid cell lines was found to lose biologic activity on ...
  http://www.biomedsearch.com/cluster/42/Immunology/sub-18-p15.html
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
We conclude that the 3D collagen matrix migration assay is a versatile method to analyze the migration of cells within a ... Various cell types could be analyzed using this technique, including lymphocytes/leukocytes, stem cells, and tumor cells. ... Within the past years various cell migration assays have been developed to analyze the migratory behavior of different cell ... We exogenously expressed a G3 construct in 66c14 cells and analyzed its effects on cell proliferation, migration, cell cycle ...
  https://www.jove.com/visualize/abstract/21079779/versican-g3-promotes-mouse-mammary-tumor-cell-growth-migration
*  Chemotaxis Assays for Eukaryotic Cells - Current Protocols
Chemotaxis is a complex response of a cell to an external stimulus ... Chemoattractant receptor crosstalk as a regulatory mechanism in leukocyte adhesion and migration. Eur. J. Immunol. 27:2571‐2578 ... J. Cell Physiol. 135:355‐366. Jungi, T.W. 1975. Assay of chemotaxis by a reversible Boyden chamber eliminating cell detachment ... Measurement of the chemotaxis coefficient for human neutrophils in the under‐agarose migration assay. Cell Motil. Cytoskeleton ...
  http://www.currentprotocols.com/WileyCDA/CPUnit/refId-cb1201.html
*  More articles from Cell Migration and Adhesion | The Journal of Immunology
Development of a high throughput cell migration assay using the SRU BIND reader (102.13) Kathryn McAllister, Marla Abodeely, ... The VCAM-1 cytoplasmic domain is essential for endothelial cell signaling and leukocyte trafficking. (102.17) Michelle Marchese ... S100A4 is dispensable for T cell development and steady state lymph node entry and migration (102.15) Yi-Chun Chen and Matthew ... Loss of T cell CD98hc specifically ablates T cell clonal expansion and protects from autoimmunity. (102.16) Joseph Cantor, ...
  http://www.jimmunol.org/content/by/section/Cell%20Migration%20and%20Adhesion
*  Protocols and Video Articles Authored by Lixin Liu
Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy' ... endothelial cells and wild-type leukocytes, neutrophil transendothelial migration out of postcapillary venules is markedly ... Using short- and long-term in vitro assays, as well as an in vivo chemotaxis assay, we investigated the importance of PI3K in ... PI3Kgamma, the class I(B) PI3K, expressed prominently in leukocytes and also in endothelial cells, mediates leukocyte ...
  https://www.jove.com/author/Lixin_Liu
*  Peptide Inhibitor of CXCL4-CCL5 Heterodimer Formation, MKEY, Inhibits Experimental Aortic Aneurysm Initiation and Progression |...
Because of the heterogeneity of the donor cells used in the in vivo short-term leukocyte migration assays, the efficacy of ... In Vivo Leukocyte Migration Assays. Donor leukocytes were isolated from spleens and bone marrow of mice 2 weeks after PPE ... CCL5 Expression Influences Transmural Aortic Leukocyte Migration in Aneurysmal Aortae. In vivo short-term leukocyte migration ... Leukocyte migration in experimental abdominal aortic aneurysm (AAA). A mixed population of spleen and bone marrow cells from ...
  http://atvb.ahajournals.org/content/33/4/718.long
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
We conclude that the 3D collagen matrix migration assay is a versatile method to analyze the migration of cells within a ... Various cell types could be analyzed using this technique, including lymphocytes/leukocytes, stem cells, and tumor cells. ... Within the past years various cell migration assays have been developed to analyze the migratory behavior of different cell ... cell migration, quantitative cell movement, microscopy, motility, assay ...
  https://www.jove.com/visualize/abstract/24849800/differential-migration-activation-profile-monocytes-after-trophoblast
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
We conclude that the 3D collagen matrix migration assay is a versatile method to analyze the migration of cells within a ... Various cell types could be analyzed using this technique, including lymphocytes/leukocytes, stem cells, and tumor cells. ... Within the past years various cell migration assays have been developed to analyze the migratory behavior of different cell ... Adult Stem Cells, Pluripotent Stem Cells, Germ Cells, Oocytes, Reproductive Physiological Processes, Stem cell, skin, germ cell ...
  https://www.jove.com/visualize/abstract/25874863/correction-quantitative-analysis-ligand-egfr-interactions-platform
*  Immunology Applications
An Improved Assay System for Cell Migration An Improved Assay System for Cell Migration Corning® FluoroBlok™ cell culture ... insights into the progression of inflammation by identifying factors that regulate directional migration of leukocytes. With ... In Vitro Assay for Neutrophil Migration In Vitro Assay for Neutrophil Migration This bulletin presents data on an in vitro ... Corning FluoroBlock Cell Culture Inserts. Improve cell migration and invasion assays with fluorescence-blocking membranes and ...
  https://www.corning.com/cala/en/products/life-sciences/applications/cell-culture/immunology-applications.html
*  Plus it
... vascular endothelial cells; and CD45, leukocytes. See also Supplementary Fig. S5. B, Migration assays were performed as ... migration assays, wound-healing assays, and cell proliferation assays were performed using standard protocols. For details, see ... Paralemmin-1 is expressed in lymphatic endothelial cells and modulates cell migration, cell maturation and tumor ... or transfected with PlexinB shRNA and then used for the migration assays (C) and cell proliferation assays (D) as described in ...
  http://clincancerres.aacrjournals.org/content/23/1/214
*  An In Vitro Inverted Vertical Invasion Assay to Avoid Manipulation of Rare or Sensitive Cell Types
Cell migration and invasion assays. Methods. 2005;37(2):208-215 7. Baharvand H, Hashemi SM, Kazemi Ashtiani S, Farrokhi A. ... The cancer cell-leukocyte fusion theory of metastasis. Adv Cancer Res. 2008;101:397-444 ... Invasion assay designs. a) Traditional Boyden Chamber or transwell invasion assay. In this assay, cells are collected from ... Traditional cell-based invasion assays like the transwell or Boyden Chamber assay require excess manipulation of the cells that ...
  http://www.jcancer.org/v07p2333.htm
*  Patent US5470739 - Cell culture support having patterned substance that influences cell adhesion - Google Patents
Collagen specifically affects the cell adhesion rate or the morphology of the cells to be adhered to the support. The steps of ... is developed for purposes of providing a cell culture support for adherent cells. ... Further, the cell culture support is produced by pretreating the support surface with a reagent to provide hydrophobicity to ... applying a solution to affect cell adhesiveness, film formation and dissolving the remaining photoresist are carried out ...
  http://www.google.com/patents/US5470739?dq=6,600,481
*  Proteases in angiogenesis - Wikipedia
Angiogenesis requires the migration and invasive growth of cells. This is facilitated by a balanced interplay between ... For example, leukocytes complex urokinase (uPA), urokinase receptor (uPAR), and integrins which participate in cell adhesion ... Both enzymes inhibit bFGF induced vascularization in the corneal pocket assay and inhibit VEGF induced angiogenesis in the ... Activated c-kit is then able to recruit hematopoietic, endothelial and mast cell progenitor cells, these cells are then ...
  https://en.wikipedia.org/wiki/Proteases_in_angiogenesis