*  Cell culture substrate "diXCOAT" | KISCO
Collaborating with the Tokyo University Industrial Technology center, KISCO developed original cell culture substrates based on diX coating technology.. ...
*  Development of 2D and 3D Culture Systems for Lens Epithelial Expansion and Differentiation | IOVS | ARVO Journals
Purpose: We currently have few techniques for expanding and maintaining the phenotype of primary lens epithelial cells (LECs) and for controlling their differentiation into 3D lens-like tissues in vitro. These limitations greatly inhibit our ability to perform basic biological studies on the lens. Therefore, the goal of this study is to determine factors that will allow for primary lens epithelial cell expansion in the absence of native matrices and to begin creating a 3D culture system that mimics the environment of the developing lens.. Methods: 2D Culture: We isolated lens epithelial cells (LECs) from the lens capsules of mice using dispase and TrypLE. The LECs were then cultured in the presence or absence of small molecules such as a TGF-beta pathway inhibitor and a Wnt pathway agonist. Cell morphology and the expression of LEC markers were then examined by ...
483575805 - EP 2980202 B1 2017-10-11 - CELL CULTURE APPARATUS AND CELL CULTURE METHOD - [origin: EP2980202A1] An apparatus for culturing cells of the present invention is an apparatus for culturing cells using a culture medium in a culture vessel. The apparatus includes: pump (4) connected to pipet (2) held by first holder (3); first shifter (5) configured to shift first holder (3); second holder (11) configured to hold culture vessel (10); second shifter (16) configured to grip and shift culture vessel (10); and controller (15). Controller (15) is programmed to, when pipet (2) draws a liquid in culture vessel (10) or discharges a liquid into culture vessel (10), control second shifter (16) to translationally shift lid (10a) of culture vessel (10) in a horizontal direction.[origin: ...
*  3D Cell Culture : Pharmaceuticals : All
SMi Group proudly presents the inaugural launch of 3D Cell Culture, an exciting new conference scheduled to take place on the 22nd and 23rd February 2017, London UK. Following the success of our Advances in Cell Based Assays series, 3D Cell Culture is set to become a leading event for industry. 3D Cell Culture 2017 will address the latest developments of 3D cell culture techniques; the ways in which predictive 3D models are presently paving the way to future technologies, and the ways in which they are currently revolutionizing drug discovery and screening and disease modelling. The 3D cell culture market is predicted to reach $3702.2 million by 2021* with main increase seen in novel technologies and culture methods. This event will highlight emerging technologies, like 3D and 4D bio ...
*  Quasi Vivo Training - Theory and Practice of Advanced Cell Culture Technique
Quasi Vivo Training - Theory and practice of advanced cell culture techniques through a combination of lectures, demonstrations and practical experience.
*  Three-dimensional cell culture: a rapidly emerging technique for drug discovery
The past decades have witnessed significant efforts toward the development of three-dimensional (3D) cell cultures. Today, 3D cell cultures are emerging not only as a new tool in early drug discovery, but also as potential therapeutics to treat disease.
*  Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors | Protocol
We describe a chip-based platform for the three-dimensional cultivation of cells in micro-bioreactors. One chip can house up to 10 Mio. ...
*  Cell Culture Training | Wright State University
This is a two day course that focuses on the introduction of the basic principal and techniques of cell culture. The course will cover cell culture definition, cell culture types, cell culture environment, cell culture wares and the protocols for cell culture techniques. In addition, there will be hands-on teaching of cell culture techniques which will include making cell culture medium, changing medium, thawing cells, freezing cells, sub-culturing cells and counting cell numbers.
*  The role of Prkci in stem cell maintenance and cell polarity using a 3-D culture system :: University of Southern California...
Atypical PKCs (aPKC) (Prkci and Prkcz) are key signaling components that have been demonstrated to control asymmetric cell division and apical‐basal polarity in all animals. aPKCs can be distinguished from other members of the PKC gene family by the presence of only a single copy of the cysteine‐rich, zinc finger‐like motif in the C1 domain. In addition, unlike other PKCs, aPKCs are not activated by DAG or calcium. However, the importance of this molecule has not been studied during mouse development. I have observed that loss of Prkci results in a failure of the early mouse embryo to undergo cavitation, with the formation of multiple luminal structures. In order to better understand the requirement for Prkci in mammalian cells, I have employed an in vitro system, embryoid body (EB) formation that mimics this embryonic phenotype. Using this system I find that loss of Prkci leads to the expansion of pluripotent populations within EBs. These pluripotent cells ...
*  In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2 | Journal of...
We have demonstrated here that CTL007 migrate through a 500 μm collagen/fibroblast separating layer toward tumor cells, resulting in tumor cell apoptosis. We have also shown that migration is dependent on CCR2 expressed by T cells and CCL2 secreted by tumor cells.. Our recently developed novel three-dimensional culture system offers a unique way of studying migration of leukocytes toward tumor cells and the factors that influence leukocyte migration under physiological conditions [32, 34]. As described in our previous studies, human CRC is grown in vitro under three-dimensional conditions using a mixture of collagen and fibroblasts [32, 44]. Interaction of α2 and β1 integrins on CRC-specific T cells with collagen and the presence of activated fibroblasts help to maintain Ag-specific T cells in a state of activation in absence of exogenous addition of IL-2 [36, 37]. In ...
*  Follow the Leader | Science Signaling
The epithelial-mesenchymal transition allows cancer cells to remodel the extracellular matrix and invade tissues. However, invasive squamous cell carcinoma (SCC) cells do not lose their epithelial markers, which led Gaggioli et al. to investigate how these cells invaded tissues. The authors devised a three-dimensional culture system consisting of a matrix block, containing mainly collagen and laminin, placed in culture medium with its upper surface exposed. SCC cells placed on this surface did not invade the matrix when cultured alone but did so when cultured with fibroblasts from tumors, as determined by immunohistochemical staining. Invasion by SCC cells was blocked when a thin sheet of matrix was placed between the fibroblasts and the SCC cells. Differential fluorescent labeling of SCC ...
*  Animal Cell Culture Protocols & Applications - QIAGEN
This section provides useful hints for culturing animal cells (i.e., cells derived from higher eukaryotes such as mammals, birds, and insects). It covers different types of animal cell cultures, considerations for cell culture, and cell culture protocols.
*  Plus it
The majority of in vivo models of metastasis determine the effect of experimental conditions (usually via knock-out mice) by relying on the end point of secondary tumor formation at a distal site (14). However, the steps occurring between tumor cell injection and metastatic tumor formation are effectively shrouded in these models (15). Despite advances in imaging to monitor these processes, a crucial step of metastasis-the invasion and colonization of cancer cells upon extravasation-remains an elusive target. This study describes a three-dimensional culture system developed to model this phase in vitro and establishes the efficacy of this novel system by demonstrating that cells from disparate phases of melanoma invade the matrix in a manner proportional to their known metastatic capabilities. Specifically, the highly metastatic C8161.9 stayed true to its documented in vivo behavior (21), ferociously staking claim to much of the surrounding ...
*  Gene Expression Analyses of Mouse Aortic Endothelium in Response to Atherogenic StimuliSignificance | Arteriosclerosis,...
We characterized fully and adapted a method15 to isolate ECs from mouse aortas that allowed us to profile gene expression in ECs during the early stages of atherosclerosis. This method provides a quick and efficient way to collect MAEC RNA, generally yielding RNA from a mouse in ,1 hour after harvesting, and has several advantages instead of cell culture methods. Not only do we avoid the passage and dedifferentiation issues that occur during cell culture procedures but we are able to directly assay the transcriptional state of the cells that closely represents their in vivo status. In these experiments, we were able to obtain the transcriptional state of ECs in prelesion aortas, and through the use of 1 RNA amplification, we were able to perform microarray analysis on MAECs taken from a single mouse. Moreover, we demonstrate that the whole aorta, with its endothelium intact, can be treated in cell ...
*  Spheroids and 3D Cell Culture: A Brief History
3D Cell Culture is much better at replicating in vivo environment than traditional two-dimensional cultures. Learn about the history of spheroids.
*  Patent US5153131 - High aspect reactor vessel and method of use - Google Patents
A suspension cell culture system where a culture chamber is rotatable about a horizontal axis and has a vertical large area oxygen transmissible membrane spaced a distance about 0.25 inches less than 1.0 inches from a facing vertical wall surface for effective transmission of oxygen to cells in suspension in the culture chamber. The facing vertical wall surface can be a dialysis membrane for exchange of fresh nutrient from a dialysis chamber with cell waste product in the culture chamber.
*  3D cell culture platform based on direct laser written microtowers
Scientists describe the optimization of the fabrication process for a novel, detailed, 3D cell culture platform based on direct laser written tubular microtowers and human neuronal cells.
*  National Level Workshop on Animal Cell Culture Techniques | 4-8 September 2017 ~ helpBIOTECH
This workshop is structured to provide hands-on skills in the basics of animal cell culture which includes passaging of cell lines, enumeration and testing its viability, scaffold preparation, cytotoxicity testing, karyotyping and detection of apoptosis ...
*  Z3221319 Cell Culture Techniques and Proceedures - CellBiology
Thawing - should occur quickly and cells diluted in pre-warmed culture medium to prevent any toxic effects of cryoprotectants in super-zero temperatures. Incubate and examine (phase contrast) the next day. Cells may need to be washed in media if cryoprotectant has a known adverse cytopathic effect ...
*  3D cell culture market size forecast 2017-2022 made available by top research firm - WhaTech
3D Cell Culture Market Report offered by Market Study Report gives a market overview of the 3D Cell Culture industry which covers product scope, market revenue, opportunities, growth rate, sales volumes and figures. The report also explores the worldwide players of the market and is segmented by region, type and application with forecast to 2022.
*  Patente US5478739 - Three-dimensional stromal cell and tissue culture system - Google Patentes
The present invention relates to an improved three-dimensional cell culture system in which cells are grown on a three-dimensional matrix while cycling the cultures between metabolically favorable and metabolically unfavorable (but noncytotoxic) conditions. The invention is based, at least in part, on the discovery that cycling the cultures in this manner optimizes the formation of extracellular matrix and produces an overall structure that more closely resembles naturally occurring tissue.
*  Epithelial Cell Culture Protocols | SpringerLink
Fascinating biology occurs at epithelial interfaces, whether between organism and environment or within body compartments, and many diseases inflicting huge personal and societal burdens result from d
*  Patent US5019646 - Polypeptides with fibronectin activity - Google Patents
A polypeptide which can bind heparin and promote cellular adhesion and neurite outgrowth is provided of the formula: lys-asn-asn-gln-lys-ser-glu-pro-leu-ile-gly-arg-lys-lys-thr, leu-ile-gly-arg-lys-lys-thr, tyr-arg-val-arg-val-thr-pro-lys-glu-lys-thr-gly-pro-met-lys-glu, ser-pro-pro-arg-arg-ala-arg-val-thr, trp-gly-pro-pro-arg-ala-arg-ile, or mixtures thereof. Medical devices such as prosthetic implants, percutaneous devices and cell culture substrates coated with the polypeptide composition are also provided.
*  Video 3D Cell Culture and Cancer Research
Kandice Tanner, PhD heads the Tissue Morphodynamics Unit at the Center for Cancer Research at the National Cancer Institute. Her lab focuses on the physical mechanisms of morphogenesis from single cell to tissues, in 3D cell cultures and others.
*  Linda Griffith by Adam Murphy - OpenWetWare
One of Dr. Griffith's most actively cited publication, "Capturing Complex 3D Tissue Physiology in vitro," provides guidelines and design principles when determining how to recreate tissue microenvironments using 3D tissue models. 3D tissue models allow researchers to study tissue physiology and pathology in vitro. Some of the salient factors for the adoption of 3D models include the use of human cells over animal cells, pre-clinical drug screening, and superior complexity compared to 2D culture models. Dr. Griffith the third dimension may prove critical in the study of tissue models because of the changed effects of mechanical input and cell adhesion, thereby impacting cell to cell signaling and cell contraction. Three dimensional tissue also creates a potential to observe cell morphology events only possible over large distance scales. Dr. Griffin also cites the importance ...