*  FinchTalk: 2008
Because these primers represent every combination of six base sequences, priming with these sequences produces a collection of ... EST (expressed sequence tag) sequencing was one of the first applications to use sequence data in a quantitative way. In EST ... Labels: Bioinformatics, ChIP-Seq, DNA sequence quality, DNA sequencing, FinchLab, Next Generation Sequencing, RNA-seq ... Today, with the exception of de novo sequencing, Next Gen sequencing has changed the way we use DNA sequences. We're no longer ...
  http://finchtalk.blogspot.com/2008/
*  Mouse TCR Profiling
DNA-based approaches, by contrast, do not identify TCR sequences in their translated forms, and will unavoidably yield many ... Following sequencing, read files were downsampled to 400,000 reads per sample based on the read saturation points identified ... Sequencing mRNA rather than genomic DNA specifically allows for the identification of expressed TCR sequences that have ... while still yielding sequence information for the beta chain. The TCR sequences identified were attributed to CD4+ T cells, ...
  http://www.clontech.com/US/Products/cDNA_Synthesis_and_Library_Construction/NGS_Learning_Resources/Technical_Notes/Mouse_TCR_Profiling?sitex=10020:22372:US&PEBCL1=8YMtyTMirwI3ubwu3SR3ygo7EC&PEBCL1_pses=ZGE81BB9AD9B711DEEEF71A4A84BC7988DBB572E793CBC6155A9C62D1C92FD6838647251BC98433D2FD7E7091BAEEE79845D7B323303A2C72F
*  DNA - Stock Image G110/0642 - Science Photo Library
The autoradiograms depict DNA base sequences, each band (dark blue) representing an individual base. - Stock Image G110/0642 ... The sequence of the 4 base groups (adenine, cytosine, guanine and thymine; coloured rods) along the DNA helix is unique for ... which consist of a sugar phosphate and a base group. Complementary pairing between the base groups of nucleotides on opposite ... Computer artwork of a DNA (deoxyribonucleic acid) double helix seen against autoradiograms of genetic sequences (upper right). ...
  http://www.sciencephoto.com/media/210102/view
*  Transcriptome Profiling Using Single-Molecule Direct RNA Sequencing | SpringerLink
Highly integrated single-base resolution maps of the epigenome in Arabidopsis, Cell 133, 523- 536.PubMedCrossRefGoogle Scholar ... RNA sequencing Single-molecule sequencing Transcriptome profiling Polyadenylation site mapping Springer Nature is developing a ... We have developed a direct RNA sequencing (DRS) approach, in which, unlike other technologies, RNA is sequenced directly ... Southern, E. M., Maskos, U., and Elder, J. K. (1992) Analyzing and comparing nucleic acid sequences by hybridization to arrays ...
  https://link.springer.com/protocol/10.1007%2F978-1-61779-089-8_4
*  US20160304952A1 - In situ nucleic acid sequencing of expanded biological samples - Google Patents
... to provide new methods for in situ sequencing of nucleic acids as well as new methods for fluorescent in situ sequencing ( ... The invention provides in situ nucleic acid sequencing to be conducted in biological specimens that have been physically ... reveal the current base of the amplified cDNA. Overall, 32 bases were sequenced from each amplified cDNA in the region of the ... C12Q1/6869-Methods for sequencing * C12Q1/6874-Methods for sequencing involving nucleic acid arrays, e.g. Sequencing By ...
  https://patents.google.com/patent/US20160304952A1
*  RACE for 5' and 3' cDNA ends, even for GC-rich transcripts
If you are working with only a few genes, RACE is more economical than Next-Generation Sequencing (NGS) for this purpose. ... SD Base * Yeast Media-One Hybrid * Yeast Media Two-Hybrid * YPDA & YPD Rich Media ... As such, it is important to have 5'- and 3'-sequence information in order to learn more about the different transcripts that ... Researchers often rely on Rapid Amplification of cDNA Ends (RACE) to acquire full-length sequence of RNA transcripts. ...
  http://www.clontech.com/US/Products/cDNA_Synthesis_and_Library_Construction/RACE_Rapid_Amplification_of_cDNA_Ends/RACE_Overview?sitex=10020:22372:US&PEBCL1=suphWgtkLuiwQDG0gCLXVRGQzV&PEBCL1_pses=ZG758E3CEF3953A99FA57F664ADFADF91C2C45283788F9F2473AD4AAA0A881D5B2BEB7F37271BB7FFB8076011C9558A77872DC88C2C19E4D62
*  Patent US4675283 - Detection and isolation of homologous, repeated and amplified nucleic acid ... - Google Patents
This approach permits detection of selectively amplified DNA sequences and identification of the DNA sequences that have been ... the DNA is denatured in situ and allowed to reanneal within the gel so that homologous DNA sequences present within restriction ... A novel method for detecting and isolating DNA sequences commonly held by different DNA preparations or repeated or amplified ... a non-repeated sequence of a few hundred thousand to a few million base pairs in sequence. The chromosomes of eukaryotes, being ...
  http://www.google.com/patents/US4675283?dq=5754119
*  Significance of strand configuration in self-replicating RNA molecules | Philosophical Transactions of the Royal Society B:...
... independent of most of the underlying base sequence. Occurrence of self-annealed strands in the Qβ replicase system was ... A 'configuration before sequence' path to self-replication exhibited a substantially lower combinatorial barrier than standard ... and a lack of homology in the internal sequence of RNA variants copied by Qβ replicase support the conclusion that template ... sequence-dependent evolution. RNA-dependent RNA synthesis in the Qβ system thus displays features of an RNA World and, ...
  http://rstb.royalsocietypublishing.org/content/350/1334/345
*  THE EVOLUTION LIST: March 2010
... assuming a three-base reading frame), then that the DNA sequence does indeed contain "meaningful" information: it is encoded in ... If the DNA sequence ACA is located in the template strand of an actively transcribed DNA sequence (i.e. a DNA sequence ... If, for example, this sequence is part of a longer sequence of nucleotides in a longer DNA molecule, then there are several ... 2) the DNA nucleotide sequence ACA could be hydrogen bonded to the complementary sequence TGT (i.e. "thymine-guanine-thymine") ...
  http://evolutionlist.blogspot.co.nz/2010/03/
*  A Molecular Biology Glossary
... the sequence of a DNA is a buzz word for the structure of a DNA molecule, in terms of the sequence of bases it contains. As a ... See also Shotgun sequencing.. Shotgun sequencing: A way of determining the sequence of a large DNA fragment which requires ... Consensus sequence: A nominal sequence inferred from multiple, imperfect examples. Multiple lanes of shotgun sequence can be ... in bases or base pairs). See "Gel Electrophoresis". This is the gel of choice for DNA or RNA in the range of thousands of bases ...
  http://seqcore.brcf.med.umich.edu/sites/default/files/html/educ/dnapr/mbglossary/mbgloss.html
*  BiO BB] Web Based Sequence Manipulations
... hz5 at njit.edu hz5 at njit.edu Tue Jun 11 13:35:46 EDT 2002 *Previous message (by ... I am hoping that your collective brains can help me find a web based , software suite to do some simple DNA sequence ... We will complete the class by cloning and sequenceing a gene, , and , we would like to do some sequence analysis with the ... Simply , looking at the sequence, translation, rev/comp sorts of things. , However, , we don't really want to install software ...
  http://bioinformatics.org/pipermail/bbb/2002-June/000729.html
*  Base Sequence Analysis
... A method, sometimes automated, for determining the base sequence.. Selected base sequence analysis ...
  http://healthboard.com/Encyclopedia/Biotech/term/base_sequence_analysis.html
*  Numerical Characterization of DNA Sequence Based on Dinucleotides
"Analysis of similarity/dissimilarity of DNA sequences based on nonoverlapping triplets of nucleotide bases," Journal of ... Numerical Characterization of DNA Sequence Based on Dinucleotides. Xingqin Qi,1 Edgar Fuller,2 Qin Wu,3 and Cun-Quan Zhang2 ... Z. H. Qi and X. Q. Qi, "Numerical characterization of DNA sequences based on digital signal method," Computers in Biology and ... B. Liao, R. Li, W. Zhu, and X. Xiang, "On the similarity of DNA primary sequences based on 5-D representation," Journal of ...
  https://www.hindawi.com/journals/tswj/2012/104269/ref/
*  Sequence-Based Strain Typing (SLST/MLST) | Charles River
... or single-locus sequence typing uses standard molecular biological methods of DNA extraction, PCR amplification and DNA ... sequencing of protein coding target genes to characterize your isolates to the strain level. ... Our sequence-based strain typing can help you map your microbial environment. We can create a library database of sequences ... Methods for sequence-based strain typing. Microbial identification using the rRNA regions is a robust technology. There are ...
  https://www.criver.com/products-services/qc-microbial-solutions/microbial-id-strain-typing/strain-typing-methods/sequence-based-strain-typing
*  HTG Molecular Launches Next-Generation Sequencing-Based Immuno-Oncology Assay
... Published 7:30 AM ET Thu, 1 Oct 2015 Globe ... These forward-looking statements are based upon management's current expectations, are subject to known and unknown risks, and ... HTG EdgeSeq Immuno-Oncology Assay couples HTG's proprietary nuclease protection chemistry with next-generation sequencing (NGS ... system capabilities have been expanded to fully automate sample and targeted library preparation for next-generation sequencing ...
  https://www.cnbc.com/2015/10/01/globe-newswire-htg-molecular-launches-next-generation-sequencing-based-immuno-oncology-assay.html
*  persistent-vector: A persistent sequence based on array mapped tries
... [ bsd3, data, library ] [ Propose Tags ] ... Strict) left folds over persistent vectors are faster than left folds over Sequences. Right folds over Sequences are faster ... This package provides persistent vectors based on array mapped tries. The implementation is based on the persistent vectors ... Updating an element at an index in a vector is slower than updating an index in a Sequence (but still faster than an IntMap). ...
  https://hackage.haskell.org/package/persistent-vector
*  Optimization of Proving Ground Durability Test Sequence Based on Relative Damage Spectrum
Citation: P, P., J, P., and Palanisamy, K., "Optimization of Proving Ground Durability Test Sequence Based on Relative Damage ... Optimization of Proving Ground Durability Test Sequence Based on Relative Damage Spectrum 2018-01-0101. ... Hence the PG test sequence must be optimal enough such that it includes all the range of frequencies representing the actual ... PG validation requires a specific durability test sequence for every segment of commercial vehicles due to different customer ...
  https://www.sae.org/publications/technical-papers/content/2018-01-0101/
*  A DNA sequence-based identification checklist for Taiwanese chondrichthyans
... Dataset homepage. Citation. Naylor G J P (2012). A ... DNA sequence-based identification checklist for Taiwanese chondrichthyans. Plazi.org taxonomic treatments database. Checklist ... This dataset contains the digitized treatments in Plazi based on the original journal article Straube, Nicolas, White, William ... T., Ho, Hsuan-Ching, Rochel, Elisabeth, Corrigan, Shannon, Li, Chenhong, Naylor, Gavin J. P. (2013): A DNA sequence-based ...
  https://www.gbif.org/dataset/0486185a-4e5f-4536-8486-78389f2699d7
*  Reduced Representation Libraries from DNA Pools Analysed with Next Generation Semiconductor Based-Sequencing to Identify SNPs...
The identification of polymorphisms was based on the construction and sequencing of two RRLs generated from DNA pools of pigs ... Ion Torrent Sequencing. Sequencing of the two RRLs was obtained using 200 ng of DNA that was purified by agarose gel ... Sequencing Data and Identification of SNPs. A total of 3,390,796 and 3,731,776 sequenced reads were obtained from the two RRLs ... Reduced Representation Libraries from DNA Pools Analysed with Next Generation Semiconductor Based-Sequencing to Identify SNPs ...
  https://www.hindawi.com/journals/ijg/2015/950737/
*  Reduced Representation Libraries from DNA Pools Analysed with Next Generation Semiconductor Based-Sequencing to Identify SNPs...
Reduced Representation Libraries from DNA Pools Analysed with Next Generation Semiconductor Based-Sequencing to Identify SNPs ... Table S3: Overlapping positions between sequencing and PorcineSNP60 Beadchip genotyping data.. Table S4: List of the 54 SNPs ... Table S1: Number of sequenced reads and nucleotides mapped on the different pig chromosomes. ...
  https://www.hindawi.com/journals/ijg/2015/950737/sup/
*  PLOS ONE: Secondary Structure, a Missing Component of Sequence-Based Minimotif Definitions
However, a longstanding problem is that the different abstractions of the sequence definitions do not accurately capture the ... and review of the literature support a new model for minimotif definitions that includes sequence, structure, and function. ... Secondary Structure, a Missing Component of Sequence-Based Minimotif Definitions. Table 1. Examples of Minimotifs with known ... PLOS is a nonprofit 501(c)(3) corporation, #C2354500, and is based in San Francisco, California, US ...
  http://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0049957.t001
*  US Patent # 5,405,747. Method for rapid base sequencing in DNA and RNA with two base labeling - Patents.com
Bases modified to accept fluorescent labels are used ... Method for rapid-base sequencing in DNA and RNA with two-base ... Method for rapid base sequencing in DNA and RNA with two base labeling Abstract. Method for rapid-base sequencing in DNA and ... Bases modified to accept fluorescent labels are used to replicate a single DNA or RNA strand to be sequenced. The bases are ... For three bases, three sequences of two bases each are required. FIG. 3a illustrates two fluorescent dyes attached through ...
  http://patents.com/us-5405747.html
*  Sequence Based Gene Expression Analysis | Springer for Research & Development
Matukumalli L.K., Schroeder S.G. (2009) Sequence Based Gene Expression Analysis. In: Edwards D., Stajich J., Hansen D. (eds) ... Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence. Nature ... Boguski MS, Lowe TM, Tolstoshev CM (1993) dbEST - database for "expressed sequence tags". Nat Genet 4:332-333CrossRefPubMed ... Camargo AA, Samaia HP, Dias-Neto E, Simao DF, Migotto IA, Briones MR et al (2001) The contribution of 700,000 ORF sequence tags ...
  https://rd.springer.com/chapter/10.1007/978-0-387-92738-1_9
*  UWB M-sequence based radar technology for localization of first responders - IEEE Conference Publication
Current solutions for localization of first responders are mainly based on fusion of multiple sensors and communication units. ...
  https://ieeexplore.ieee.org/document/8008218/
*  Training Sequence Based Frequency-Domain Channel Estimation for Indoor Diffuse Wireless Optical Communications - TechRepublic
Based on the Training Sequence (TS), this paper ... ... Based on the Training Sequence (TS), this paper develops three ... Training Sequence Based Frequency-Domain Channel Estimation for Indoor Diffuse Wireless Optical Communications. ...
  https://www.techrepublic.com/resource-library/whitepapers/training-sequence-based-frequency-domain-channel-estimation-for-indoor-diffuse-wireless-optical-communications/