Crystal structure of a mouse Tankyrase-Axin complex ... 3D Structures in the Protein Data Bank *Computed Structure ... Axin is a tumor suppressor and a key negative regulator of the Wnt/β-catenin signaling pathway. Axin turnover is controlled by ... The bivalent binding of Axin to TNKS is required for Axin turnover, since mutations in either gate-binding glycine residue in ... Crystal structure of a Tankyrase-Axin complex and its implications for Axin turnover and Tankyrase substrate recruitment.. ...

Schwarz-Romond T, Metcalfe C, Bienz M (July 2007). "Dynamic recruitment of axin by Dishevelled protein assemblies". Journal of ... In addition to protein recruitment, condensates can also be designed which release proteins in response to certain stimuli. In ... Membrane protein, or membrane-associated protein, clustering at neurological synapses, cell-cell tight junctions, or other ... In one approach, which localizes condensates to specific genomic regions, core proteins are fused to proteins such as TRF1 or ...

Protein Aliases: Axam; Axam2; Axin-associating molecule; CT; Sentrin-specific protease 2; Sentrin/SUMO-specific protease SENP2 ... protein transport Wnt signaling pathway protein sumoylation protein desumoylation regulation of Wnt signaling pathway negative ... positive regulation of protein ubiquitination protein destabilization negative regulation of protein binding regulation of DNA ... SUMO is a small ubiquitin-like protein that can be covalently conjugated to other proteins. SENP2 is one of a group of enzymes ...

SUMOylation is a post-translational modification of proteins that has been found to play a major role in the Wnt/β-catenin ... SUMOylation is a post-translational modification of proteins that has been found to play a major role in the Wnt/β-catenin ... SUMOylation of Axin has No Effect on β-catenin Rather Than JNK. Axin is a multi-domain protein that interacts with several ... SUMOylation of the same protein may result in opposite regulation of different downstream effector proteins. The same protein ...

Cyclin D1+beta Catenin+GSK3 beta+LEF1+Axin 2 (1). * DAPK2+Src+TrkA+FER+c-Kit+PAK1 + PAK2 + PAK3+PDPK1+EGFR+GSK3 beta+AMPK alpha ... Proteins and Peptides. Proteomics tools. Agonists, activators, antagonists and inhibitors. Cell lines and Lysates. Multiplex ...

Strovel, E. T., Wu, D. & Sussman, D. J. Protein phosphatase 2Cα dephosphorylates axin and activates LEF-1-dependent ... Cell 12, 261-274 (2007). A requirement for intact microtubules and the motor protein kinesin-1 for efficient SMAD2 ... The MAD-related protein SMAD7 associates with the TGFβ receptor and functions as an antagonist of TGFβ signaling. Cell 89, 1165 ... Ge, G. & Greenspan, D. S. BMP1 controls TGFβ1 activation via cleavage of latent TGFβ-binding protein. J. Cell Biol. 175, 111- ...

Roles of axin in the wnt signalling pathway. Cell. Signalling, 11(11), 777-788. ... 2Protein phosphatase 2A (PP2A) is a very abundant - it accounts for as much as 1% of total cellular proteins - ubiquitous and ... WD40 repeat proteins striatin and S/G(2) nuclear autoantigen are members of a novel family of calmodulin-binding proteins that ... Protein Phosphatase 2A (PP2A) is a widely expressed family of protein phosphatases made of a core dimer, composed of a ...

Axin (Scaffold protein). *Azalea lace bug. *Bacteriophage phi 6. *Barbicans. *Barnacle populations ...

Recombinant Human Wnt-3a Protein. Backed by our 100% Guarantee. ... is a cell-permeant small molecule inhibitor of Axin turnover ... XH Jiang, YT Xie, YP Cai, J Ren, T Ma Effects of hepatitis C virus core protein and nonstructural protein 4B on the Wnt/?- ... Recombinant Human Wnt-3a Protein Summary. Details of Functionality. Measured by its ability to induce alkaline phosphatase ... Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line.. Optimal ...

... which leads to increased stabilization of the Axin protein in the destruction complex leading to the general degradation of β- ... The proteins were resolved with electrophoresis on 12% SDS-polyacrylamide gels (20 μg protein/lane). The proteins were then ... Block of proliferation 1 protein (BOP1; OMIM 610596) and CDC28 Protein Kinase Regulatory Subunit 2 (CKS2; OMIM 116901) have ... EMT marker proteins without sacrificing the levels of the prosurvival protein VEGF. These findings support the development of a ...

Aberrant expression and activity of G proteins and G-protein-coupled receptors (GPCRs) are frequently associated with ... This Analysis article reviews these findings and the indications that G proteins, GPCRs and their signalling pathways represent ... These studies indicate that G proteins, GPCRs and their linked signalling circuitry represent novel therapeutic targets for ... Aberrant expression and activity of G proteins and G-protein-coupled receptors (GPCRs) are frequently associated with ...

The cysts are often filled with fluid of a high protein content (thus, they are hyperintense on T1-weighted images). Solid ... with activation of downstream targets such as Axin-2. [9, 30] Papillary craniopharyngiomas and other sellar region lesions do ... Tight junction protein claudin-1 is differentially expressed in craniopharyngioma subtypes and indicates invasive tumor growth ... Expression of enamel proteins and LEF1 in adamantinomatous craniopharyngioma: evidence for its odontogenic epithelial ...

The protein stability of Axin, a negative regulator of Wnt signaling, is regulated by Smad ubiquitination regulatory factor 2 ( ... Degradation of AXIN (Homo sapiens) * Ubiquitinated AXIN is degraded by the proteasome (Homo sapiens) ... Ubiquitinated AXIN is degraded by the proteasome (Bos taurus) Ubiquitinated AXIN is degraded by the proteasome (Canis ... Ubiquitinated AXIN is degraded by the proteasome (Danio rerio) Ubiquitinated AXIN is degraded by the proteasome (Drosophila ...

We demonstrate that DVL dishevelled and axin (DIX) domain, but not DIX domain-mediated multimerization, is essential for DVLs ... and centrosomal Nek2-associated protein 1 (C-NAP1), two proteins of the centrosomal linker. Displacement of DVL from the ... Dishevelled (DVL) is a key scaffolding protein and a branching point in Wnt signaling pathways. Here, we present conclusive ... Dishevelled is a NEK2 kinase substrate controlling dynamics of centrosomal linker proteins. ...

β-catenin protein is an integral part of the canonical Wnt signaling pathway. Wnt binding to Frizzled (Fz) receptors and LRP co ... Mutations in β-catenin, axin and APC have been discovered in numerous cancers, and other members of the canonical pathway may ... β-catenin protein is an integral part of the canonical Wnt signaling pathway. Wnt binding to Frizzled (Fz) receptors and LRP co ... and axin. Accumulation of β-catenin in the cell cytoplasm prompts its translocation to the nucleus, where it interacts with ...

Axin Protein. , Carcinoma. , Cell Transformation, Neoplastic. , Clone Cells. , Colon. , Colorectal Neoplasms. , DNA Copy Number ...

APC, along with interacting proteins GSK-3β and Axin, actively degrades β-catenin and maintains cytoplasmic β-catenin at very ... In the context of nuclear protein trafficking, it is plausible that there is a specific transporter protein that facilitates ... the supernatants were used as the protein source. To make protein preparations that would contain only soluble cytoplasmic ... Nuclear protein (5 μg) was added to a 20-μl reaction mix containing 300 ng of poly(dI·dC); binding buffer [10 mm HEPES (pH 7.6 ...

Next, the adaptor protein NAB-1 (neurabin) binds to F-actin and recruits active zone proteins SYD-1 and SYD-2 (liprin-α) by ... Moreover, mutations in mig-5/Disheveled, gsk-3, pry-1/Axin, bar-1/beta-catenin and pop-1/TCF, also cause disrupted D-type axon ... Ca2+/calmodulin-dependent protein kinase II (CaMKII) is a serine/threonine protein kinase that regulates long-term potentiation ... The polarized distribution of neuronal proteins to axons and dendrites relies on microtubule-binding proteins such as CRMP, ...

protein complex - protein heterooligomerization - protein kinase activator activity - protein phosphorylation - protein serine/ ... These pathways are clinically prognostic and predictive, including the TP53-AXIN-ARHGEF17 combination in liver and CYLC2-STK11- ... The protein expression of AMPK, liver kinase B1 (LKB1) and NF-ĸB in tumors was examined by western blotting.. RESULTS: ... What pathways are this gene/protein implicaed in?. Show (2). STK11 is involved in:. - Adipocytokine signaling pathway KEGG. - ...

... show Dvl2 undergoes liquid-liquid phase separation to stabilize β-catenin by pulling Axin into its biomolecular condensate at ... III PhasAGE International Conference - Multiscale understanding of protein aggregation and biomolecular condensates in aging ... Letting go: Dishevelled phase separation recruits Axin to stabilize β-catenin. .avatar img { max-width: 100%; height: auto; } ...

Compounds target PPI of β-catenin with different proteins. ... as an adhesion protein and as a signaling protein, transducing ... Axin is a concentration-limiting component of the β-catenin degradation complex, and its stability is regulated by tankyrase, a ... These proteins were firstly discovered in 1989. The molecular weight of proteins were 102, 88 and 80 kDa, so they were named α ... This library represents a selection of drug-like compounds aimed at modulating protein-protein interaction (PPI) of β-catenin ...

... axin and adenomatous polyposis coli tumor suppressor proteins (APC). When Wnt ligands bind with their cognate cell membrane ... axin, the adenomatous polyposis coli tumor suppressor proteins (APC) and glycogen synthase kinase 3 (GSK3) [16]. Nevertheless, ... including people from the secreted frizzled receptor proteins (sFRP) family members, Dickkopf (Dkk) proteins [18], Wnt ... The Rho category of GTPases contains 20 members, that are Ras-like proteins. Amongst these, Cdc42, Rac1, and. ...

The Wnt signaling pathway creates a complex network of proteins that have different effects on cells, so they can be involved ... β-catenin levels will be kept lower by a set of proteins. In fact, β-catenin phosphorylation by GSK-3β, along with APC and Axin ... as one of the proteins in this pathway, showed proto-oncogenic properties, and it was found that mutations in this protein lead ... One of the most important of these proteins is β-catenin, which plays a pivotal role in the Wnt signaling pathway [6, 7]. If ...

Wnt receptor complexes should fully deactivate Axin rather than temporarily deconstruct it from other scaffold proteins, (3) in ... Deactivation of Axin by the Wnt receptor complex needs to be critically efficient relative to β-catenin removal by Axin, and (5 ... Deactivation of Axin by the Wnt receptor complex needs to be critically efficient relative to β-catenin removal by Axin, and (5 ... Wnt receptor complexes should fully deactivate Axin rather than temporarily deconstruct it from other scaffold proteins, (3) in ...

8A). We next detected APC, β-catenin, GSK-3β, Axin, and p-GSK-3β proteins in the pathway by Western Blot. The analyses showed ... The models of NRP-1, Wnt1, β-catenin, GSK-3β, Axin, APC were acquired from the Uniport Protein Data Bank [35]. The structures ... The concentration of protein was measured with a BCA Protein Assay Kit (Cat# 23227; Thermo Fisher Scientific, MA, USA). An ... The overexpression of NRP-1 increases the expression of Wnt proteins. Wnt proteins are released to the extra-cellular space for ...

Inhibition of PP1 leads to enhanced phosphorylation of specific sites on axin by casein kinase I. Axin phosphorylation markedly ... "Protein phosphatase 1 regulates assembly and function of the beta-catenin degradation complex." EMBO J, vol. 26, no. 6, Mar. ... Protein phosphatase 1 regulates assembly and function of the beta-catenin degradation complex.. Publication , Journal Article ... "Protein phosphatase 1 regulates assembly and function of the beta-catenin degradation complex." EMBO J 26, no. 6 (March 21, ...

The product of the Fused locus, Axin, displays similarities to RGS (Regulators of G-Protein Signaling) and Dishevelled proteins ... The product of the Fused locus, Axin, displays similarities to RGS (Regulators of G-Protein Signaling) and Dishevelled proteins ... The product of the Fused locus, Axin, displays similarities to RGS (Regulators of G-Protein Signaling) and Dishevelled proteins ... The product of the Fused locus, Axin, displays similarities to RGS (Regulators of G-Protein Signaling) and Dishevelled proteins ...

Loss-of-function mutations in APC or AXIN or activating mutations in the gene encoding β-catenin, CTNNB1, lead to aberrant ... 1997) Role of translocation in the activation and function of protein kinase B. J Biol Chem 272: 31515-31524. * View Article ... In the absence of Wnt ligands, a complex containing APC, AXIN and GSK3 phosphorylates β-catenin, marking it as a substrate for ... Ko HW, Jiang J, Edery I (2002) Role for Slimb in the degradation of Drosophila Period protein phosphorylated by Doubletime. ...

G-protein coupled receptors (GPCRs) possess characteristics that make them ideal targets for molecular imaging and therapeutics ... While expression patterns of many proteins in human medulloblastoma subgroups have been discerned, the expression pattern of ... Prostaglandin E2 promotes colon cancer cell growth through a Gs-axin-beta-catenin signaling axis. Science 2005,310(5753):1504- ... Fredriksson R, Schioth HB: The repertoire of G-protein-coupled receptors in fully sequenced genomes. Mol Pharmacol 2005, 67: ...

Tankyrase ADP-ribosylates AXIN (Mus musculus) * RibC-AXIN:TNKS [cytosol] (Mus musculus) * RibC-AXIN [cytosol] (Mus musculus) * ... Metabolism of proteins (Mus musculus) * * Post-translational protein modification (Mus musculus) * Deubiquitination (Mus ... RibC-AXIN:TNKS:RNF146 [cytosol] (Mus musculus) * RibC-AXIN:TNKS [cytosol] (Mus musculus) * RibC-AXIN [cytosol] (Mus musculus) * ... RibC-AXIN:TNKS:RNF146 [cytosol] (Mus musculus) * RibC-AXIN:TNKS [cytosol] (Mus musculus) * RibC-AXIN [cytosol] (Mus musculus) * ...