Metallothionein-3 modulates the amyloid β endocytosis of astrocytes through its effects on actin polymerization | Molecular...
Primary astrocytes plated on cover slips were stained with 5 μg/ml Alexa Fluor 594-WGA for 10 min at 37 °C. Stained astrocytes ... Actin disruption reduces Aβ uptake by astrocytes. a, b Confocal fluorescence micrographs of WT astrocytes were sham washed or ... Protein expression levels were measured in whole-cell extracts of cultured cortical astrocytes. Astrocytes obtained from both ... Although vehicle-treated control astrocytes exhibited a typical fibrillary pattern of actin staining (phalloidin), astrocytes ...https://molecularbrain.biomedcentral.com/articles/10.1186/s13041-015-0173-3
Morphological evidence for vesicular glutamate release from astrocytes. - Semantic Scholar
Astrocytes may respond to neuronal activity by such exocytotic release of glutamate. The astrocyte derived glutamate can in ... One transmitter that in a vast number of studies has been shown to be released from astrocytes is glutamate. Although ... Here we review the morphological data supporting that astrocytes possess the machinery for exocytosis of glutamate. We describe ... the presence of small synaptic-like microvesicles, SNARE proteins and vesicular glutamate transporters in astrocytes, as well ...https://www.semanticscholar.org/paper/Morphological-evidence-for-vesicular-glutamate-rel-Bergersen-Gundersen/3a8a513fd96cf3fd73337ee789834f63ed31dd3b
"Intracellular pH Regulation in Primary Rat Astrocytes and C6 Glioma Ce" by Lamara D. Shrode and Robert W. Putnam
Astrocytes, but not C6 cells, contain a fourth pH-regulating transporter, Na+ −HCO3− cotransport; in the presence of CO2, ... regulation in primary cultures of rat astrocytes and C6 glioma cells. Both cell types contain three pH-regulating transporters ... depolarization-induced alkalinization of astrocytes was seen only in the presence of CO2. ... Astrocytes, but not C6 cells, contain a fourth pH-regulating transporter, Na+ −HCO3− cotransport; in the presence of CO2, ...https://corescholar.libraries.wright.edu/ncbp/758/
Loss of Astrocyte Polarization in the Tg-ArcSwe Mouse Model of Alzheimer's Disease
... making AQP4 an excellent marker of astrocyte polarization. Loss of astrocyte polarization is known to compromise astrocytic ... Loss of Astrocyte Polarization in the Tg-ArcSwe Mouse Model of Alzheimer's Disease. Yang, Jing ... Thus, astrocytes exhibit a redistribution of AQP4 from endfoot membranes to non-endfoot membrane domains. The present data ... Aquaporin-4 (AQP4) is the predominant water channel in brain and is selectively expressed in astrocytes. Astrocytic endfoot ...http://uu.diva-portal.org/smash/record.jsf?pid=diva2:471282
Circadian Regulation of ATP Release in Astrocytes | Journal of Neuroscience
Astrocyte circadian rhythms depend on clock genes. To assess the molecular basis of circadian timing in astrocytes, we tested ... Astrocytes were derived from mice of different genotypes to assess whether the molecular basis of daily timing in astrocytes is ... 1A,B). Thus, ATP release from murine cortical astrocytes is circadian, consistent with findings in rat astrocytes, immortalized ... For comparison with dnSNARE and VIPP astrocytes, we used astrocytes from wild-type mice or littermates expressing only the ...http://www.jneurosci.org/content/31/23/8342
Binding and Uptake of A beta 1-42 by Primary Human Astrocytes In Vitro
Thus, primary human astrocytes derived from different sources can bind and internalize A beta 1-42, and fetal astrocytes were ... Thus, primary human astrocytes derived from different sources can bind and internalize A beta 1-42, and fetal astrocytes were ... In vitro studies confirmed that A beta is taken up by rodent astrocytes, but knowledge on human astrocyte-mediated A beta ... In vitro studies confirmed that A beta is taken up by rodent astrocytes, but knowledge on human astrocyte-mediated A beta ...https://lup.lub.lu.se/search/publication/1443637
The induction of intercellular adhesion molecule 1 (ICAM-1) expression on human fetal astrocytes by interferon-gamma, tumor...
The best in vivo data showing that astrocytes serve as intracerebral APCs is the finding that astrocytes in multiple sclerosis ... If astrocytes function as immunocompetent APCs within the brain, it would seem that they would also be able to express ... Here, we present the first data that indicates that human astrocytes are capable of expressing ICAM-1 in response to cytokines ... This hypothesis is based upon the ability to induce the expression of the class II MHC antigens on astrocytes, and on their ...https://www.ndorms.ox.ac.uk/publications/482693
北京大学医学部机构知识库(IR@PKUHSC): The association of 14-3-3 gamma and actin plays a role in cell division and apoptosis in astrocytes
The association of 14-3-3 gamma and actin plays a role in cell division and apoptosis in astrocytes.pdf（372KB）. 期刊论文. 出版稿. 限制开放 ... 14-3-3 gamma ; actin ; astrocyte ; ischemia ; cell division ; mitosis ; apoptosis 刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH ... However, in apoptotic astrocytes, the intensity of immunostaining of 14-3-3gamma proteins in the cytoplasm decreased. The ... The association of 14-3-3 gamma and actin plays a role in cell division and apoptosis in astrocytes[J]. BIOCHEMICAL AND ...http://ir.bjmu.edu.cn/handle/400002259/67757
Different in vitro response to rIL-1 beta of newborn and adult rat astroglia. - Semantic Scholar
In contrast, rIL-1 beta induces an increase of GFAP immunoreactivity both in newborn and in adult astrocytes, as compared to ... Thus, it appears likely that different factors, other than rIL-1 beta, are needed by adult astrocytes to proliferate. ... only newborn astrocytes can respond to this lymphokine with proliferation. ... In newborn, but not in adult astrocytes, 100 U/ml of rIL-1 beta increase [3H]thymidine incorporation with a maximal response by ...https://www.semanticscholar.org/paper/Different-in-vitro-response-to-rIL-1-beta-of-newbo-Colasanti-Ramacci/d9d480c9affce6497f8eedd10ab3be3d2751fd66
astrocytes - Dwight Bergles Laboratory
Human astrocytes develop physiological morphology and remain quiescent in a novel 3D matrix.. Astrocytes are the most abundant ... Astrocyte morphology is controlled by neuron-derived FGF.. The highly ramified processes of astrocytes enable cellular ... Neuromodulators signal through astrocytes to alter neural circuit activity and behaviour.. Astrocytes associate with synapses ... Large-scale recording of astrocyte activity.. Astrocytes are highly ramified glial cells found throughout the central nervous ...http://bergleslab.com/index.php/tag/astrocytes/
The antagonistic modulation of Arp2/3 activity by N-WASP, WAVE2 and PICK1 defines dynamic changes in astrocyte morphology |...
Rat astrocytes grow as polygonal cells in serum-rich medium, so we used a previously established protocol to drive astrocytes ... Inactivation of the Arp2/3 complex evokes cell expansion in astrocytes. To study the role of the Arp2/3 complex in astrocytes, ... Dissociated astrocytes were categorized into polygonal and stellate astrocytes by calculating the ratio of cell outlines and ... To study morphological properties of astrocytes within tissue, only astrocytes in layer 2 or 3 of the motor cortex and at a ...http://jcs.biologists.org/content/126/17/3873
UNTHSC Scholarly Repository - Research Appreciation Day: HIV-1 Tat and gp120 Regulate Astrocyte Tissue Inhibitor of Matrix...
Results: HIV-1 Tat and gp120 expression in astrocytes significantly reduced cell viability. Transfected astrocytes showed ... 1 protects human neurons from HIV-1-induced apoptosis and is mainly produced by astrocytes. Further, astrocyte TIMP-1 is ... Astrocytes, the principal type of glial cells, are involved in signaling, homeostasis, and repair during CNS pathology. Some ... Methods: Primary human astrocytes were transfected with HIV-1 protein-expressing plasmids (Tat72, Tat101, gp120JR-FL). First, ...https://digitalcommons.hsc.unt.edu/rad/RAD15/Immunology/1/
Live Imaging of Neuroinflammation Reveals Sex and Estrogen Effects on Astrocyte Response to Ischemic Injury | Stroke
Astrocyte response to ischemic injury in live animals was studied in GFAP-luc mice. Previous work by Zhu and colleagues16 ... Reactive astrocytes: cellular and molecular cues to biological function. Trends Neurosci. 1997; 20: 570-577. ... Live Imaging of Neuroinflammation Reveals Sex and Estrogen Effects on Astrocyte Response to Ischemic Injury. Pierre Cordeau, ... At the early time point after OVX, we did not observe an increase in astrocyte response after MCAO, which may in part be ...http://stroke.ahajournals.org/content/39/3/935
A latent neurogenic program in astrocytes regulated by Notch signaling in the mouse | Science
Astrocytes' hidden ability to generate neurons. New neurons could be useful in the brain to respond to damage done by disease, ... Astrocytes can produce neuroblasts in response to stroke and can be artificially triggered to do so in the absence of injury. ... Astrocytes can produce neuroblasts in response to stroke and can be artificially triggered to do so in the absence of injury. ... They found that astrocytes, spidery cells that are interspersed between neurons, carry a latent neurogenic program. The ability ...http://science.sciencemag.org/content/346/6206/237
A crucial role for Olig2 in white matter astrocyte development | Development
A crucial role for Olig2 in white matter astrocyte development. Jeff Cai, Ying Chen, Wen-Hui Cai, Edward C. Hurlock, Heng Wu, ... A crucial role for Olig2 in white matter astrocyte development. Jeff Cai, Ying Chen, Wen-Hui Cai, Edward C. Hurlock, Heng Wu, ... A crucial role for Olig2 in white matter astrocyte development. Jeff Cai, Ying Chen, Wen-Hui Cai, Edward C. Hurlock, Heng Wu, ... A crucial role for Olig2 in white matter astrocyte development Message Subject (Your Name) has sent you a message from ...http://dev.biologists.org/content/early/2007/04/11/dev.02847
How might brain "astrocytes" alter nerve cell connections and contribute to Autism Spectrum Disorder?
... Investigating astrocyte- ... Key functions of astrocytes are altered in some cases of ASD, including Fragile X syndrome where astrocyte transporter function ... Research suggests that astrocytes can produce both advantageous and deleterious effects. In the developing brain, astrocytes ... Astrocytes surround neurons, and their star-shaped "processes" closely associate with synapses enabling astrocytes to ...http://www.dana.org/Media/GrantsDetails.aspx?id=123059
A model for glioma cell migration on collagen and astrocytes | Journal of The Royal Society Interface
3.2 Migration on a monolayer of astrocytes. We considered two layers in the model: one layer is that of the astrocytes on top ... 2.1.2 Primary astrocyte cultures. Primary astrocyte cultures were obtained by mechanical mincing and passing through a nylon ... In this case the astrocytes are not confluent, contrary to what happens in monolayers of astrocytes. It is likely that the ... This means that half of the time cells avoid sites above astrocytes and astrocytes act as obstacles. This explains why ...http://rsif.royalsocietypublishing.org/content/5/18/75
Glial-derived proteins activate cultured astrocytes and enhance beta amyloid-induced glial activation.
Treatment of astrocytes with ACT, IL-1beta, or S100beta resulted in glial activation, as assessed by reactive morphology, ... In contrast, BChE alone did not activate astrocytes and had no effect on Abeta-induced activation. These results suggest that ... The ability of Abeta to induce astrocyte activation was also enhanced in the presence of each of these three proteins. ... Glial-derived proteins activate cultured astrocytes and enhance beta amyloid-induced glial activation.. * ...http://www.prohealth.com/library/showarticle.cfm?libid=1723
Astrocyte - Wikipedia
The astrocytes next to neurons in the frontal cortex and hippocampus store and release glucose. Thus, astrocytes can fuel ... Astrocytes are depicted in red. Cell nuclei are depicted in blue. Astrocytes were obtained from brains of newborn mice ... Astrocytes are macroglial cells in the central nervous system. Astrocytes are derived from heterogeneous populations of ... Gömöri-positive astrocytes. These are a subset of protoplasmic astrocytes that contain numerous cytoplasmic inclusions, or ...https://en.wikipedia.org/wiki/Astrocytes
Metabolic Interactions between Neurons and Astrocytes | SpringerLink
Hertz, L. and Schousboe, A., Role of astrocytes in compartmentation of amino acid and energy metabolism. In:Astrocytes, ... Walz, W. and Hinks, E., A transmembrane sodium cycle in astrocytes, Brain Res. 368: 226-232 (1986).PubMedCrossRefGoogle Scholar ... Code, W.E., White, H.S. and Hertz, L., The effect of midazolam on calcium signaling in astrocytes, Ann. N.Y. Acad. Sci. 625: ... Yu, A.C.H., Gregory, G.A. and Chan, P.H., Hypoxia-induced dysfunction and injury of astrocytes in primary cell cultures, J. ...https://link.springer.com/chapter/10.1007/978-1-4757-9486-1_1
Astrocytes help separate man from mouse
... fibrous astrocytes »human brain »information processing »interlaminar astrocytes »protoplasmic astrocytes »spinal cord injury » ... fibrous astrocytes , human brain , information processing , interlaminar astrocytes , protoplasmic astrocytes , spinal cord ... Alzheimer's »Calcium »Epilepsy »Human astrocytes »astrocytes »blood flow »blood vessel »brain cell »brain function and sensory ... Further reports about: , Alzheimer's , Calcium , Epilepsy , Human astrocytes , astrocytes , blood flow , blood vessel , brain ...http://www.innovations-report.com/html/reports/life-sciences/astrocytes-separate-man-mouse-129901.html
A role for astrocytes in learning and memory?
Astrocytes, once thought little more than passive, structurally supportive brain cells, are increasingly recognized as having a ... have found that individual star-shaped glial cells called astrocytes release a specific signalling molecule, called D-serine, ... Long-term potentiation depends on release of D-serine from astrocytes. Nature ( doi:10.1038/nature08673).. ...http://www.ucl.ac.uk/ion/articles/archive-2010/Rusakov
Astrocytes, ATP, Brainstem, and Breathing | Science Signaling
Thus, brainstem astrocytes have the ability to sense changes in blood and brain CO2, and pH directly, and may control the ... Astrocytes (or glial cells), previously assumed to be passive players in brain physiology, may play a functional role in a ... The depolarization in these neurons is evoked by vesicular release of ATP in neighboring astrocytes in response to the fall in ... but what about astrocytes? Gourine et al. now present evidence that glial cells may help to control breathing. A number of ...http://stke.sciencemag.org/content/3/133/ec240
Astrocytes and guidance of outgro... preview & related info | Mendeley
In the present investigation the role of astrocytes and their precursors in guidance of outgrowing corticospinal tract axons in ... Astrocytes and guidance of outgrowing corticospinal tract axons in the rat. An immunocytochemical study using anti-vimentin and ... In the present investigation the role of astrocytes and their precursors in guidance of outgrowing corticospinal tract axons in ... labelling experiments it can be concluded that the vimentin-glial fibrillary acidic protein transition occurs within astrocyte ...https://www.mendeley.com/research-papers/astrocytes-guidance-outgrowing-corticospinal-tract-axons-rat-immunocytochemical-study-using-antivime/
In NeuroAIDS, Infected Astrocytes May Cause Toxicity, Trigge... : Neurology Today
These cells included endothelial cells and uninfected astrocytes. Remarkably, the infected astrocytes themselves did not ... In NeuroAIDS, Infected Astrocytes May Cause Toxicity, Triggering Apoptosis. Robinson, Richard. Neurology Today: August 04, 2011 ... Astrocytes also play a key role in maintaining the blood-brain barrier. Astrocytic "end feet" surround the blood vessels, ... Home , August 04, 2011 - Volume 11 - Issue 15 , In NeuroAIDS, Infected Astrocytes May Cause Toxicity, Trigge... ...https://journals.lww.com/neurotodayonline/Fulltext/2011/08040/In_NeuroAIDS,_Infected_Astrocytes_May_Cause.4.aspx