Apurinic Apyrimidinic (AP) endonuclease (NApe) from Neisseria Meningitidis ... We have identified and characterised two apurinic/apyrimidinic (AP) endonuclease paralogues in the human pathogen Neisseria ... Apurinic Apyrimidinic (AP) endonuclease (NApe) from Neisseria Meningitidis. *PDB DOI: https://doi.org/10.2210/pdb2JC5/pdb ...

Lindahl, T; Nyberg, B (1972). "Rate of depurination of native deoxyribonucleic acid". Biochemistry. 11 (19): 3610-8. doi: ... The enzyme DNA-(apurinic or apyrimidinic site) lyase, also referred to as DNA-(apurinic or apyrimidinic site) 5- ... In the 1970s, this class of enzyme was found to repair at apurinic or apyrimidinic DNA sites in E. coli and in mammalian cells ... Initially, AP hydrolyses, such as apurinic or apyrimidinic endonuclease I contain a Mg2+ active site that cleaves the DNA on ...

"The formation apurinic acid from the desoxyribonucleic acid of calf thymus". The Journal of Biological Chemistry. 195 (1): 49- ... acid stress).[76] A pH of 4 at body temperature of 37 °C causes depurination of DNA, leaving apurinic (AP) sites in DNA,[77] ... Evidence has shown that a fatty acid messenger, cis-2-decenoic acid, is capable of inducing dispersion and inhibiting growth of ... The EPS matrix consists of exopolysaccharides, proteins and nucleic acids.[40][41][42] A large proportion of the EPS is more or ...

Nucleic Acid Res. Online ahead of print. PMID: 32542366.. Daniel R. McNeill, Amy M. Whitaker, Wesley J. Stark, Jennifer L. ... Apurinic/apyrimidinic (AP) endonuclease 1 processing of AP sites with 5 mismatches. Acta Crystallogr D Struct Biol. 74(Pt 8): ... Nucleic Acids Res. PMID: 28449123. *Co-first Authors. Amy M. Whitaker*, Matthew A. Schaich*, Mallory S. Smith, Tony S. Flynn, ... under review at Nucleic Acids Research). Max S. Fairlamb, Amy M. Whitaker, Fletcher E. Bain, Maria Spies, Bret D. Freudenthal. ...

Yi TL, Bolen JB and Ihle JN (1991) Hematopoietic cells express two forms of lyn kinase differing by 21 amino acids in the amino ... Kanno S, Iwai S, Takao M and Yasui A (1999) Repair of apurinic/apyrimidinic sites by UV damage endonuclease; a repair protein ... Immunoprecipitates were incubated with acid-treated enolase as a positive control. As shown in Fig. 1B, autophosphorylation of ... The numbers indicate amino acid (a.a) positions in rpS3. (E) HEK293T cells were cotransfected with pEBG-rpS3 deletion mutants ...

Apurinic Acid, DNA Damage, DNA Repair, DNA-Formamidopyrimidine Glycosylase, Guanine, N-Glycosyl Hydrolases, Oligonucleotides, ...

Nucleic acids research The efficiency and specificity of apurinic/apyrimidinic site bypass by human DNA polymerase eta and ... Nucleic acids research Reduced levels of DNA polymerase delta induce chromosome fragile site instability in yeast. Kokoska RJ ...

It is a cancer vaccine based on the patients autologous DCs loaded with a fusion protein of prostatic acid phosphatase (PAP) ... or apurinic/apyrimidinic, site; A-to-I, adenosine-to-inosine RNA editing; BER, base excision repair; C, cytosine; DBD, ... generic abbreviation for the deoxyribonucleic acid, or dC-to-dU, deaminase family (APOBECs1,2,4 and human 3A/3B/ 3C/3D/3F/3G/3H ...

However, during repair of the apurinic/apyrimidinic (AP) sites or 5,6-dihydrouracil, poly(ADP-ribose) polymerase plays an ... Apurinic Acid, Cell-Free System, Cells, Cultured, DNA Glycosylases, DNA Repair, Guanine, Humans, Hypoxanthine, Lymphocytes, ... However, during repair of the apurinic/apyrimidinic (AP) sites or 5,6-dihydrouracil, poly(ADP-ribose) polymerase plays an ...

... sensitizes human cells to alkylation damage and the additional depletion of apurinic/apyrimidinic endonuclease I (APE1) confers ... Nucleic Acids Research, 42 (5). pp. 3089-3103. ISSN 0305-1048 ...

New insights in the mitochondrial localization of APE1, the major apurinic/apyrimidinic endonuclease in mammals 1-gen-2010 ... in the modulation of its interaction with nucleic acids and Nucleophosmin (NPM1) 1-gen-2013 Poletto, M; Vascotto, Carlo; ... Functional regulation of the apurinic/apyrimidinic endonuclease 1 by nucleophosmin: impact on tumor biology 1-gen-2014 Vascotto ... Role of the unstructured N-terminal domain of the human Apurinic/Apyrimidinic Endonuclease 1 (hAPE1) in the modulation of its ...

nucleic acid binding. IEP. Enrichment. MF. GO:0003906. DNA-(apurinic or apyrimidinic site) endonuclease activity. IEP. ... hydrolase activity, acting on acid anhydrides. IEP. Enrichment. MF. GO:0016818. hydrolase activity, acting on acid anhydrides, ... organic acid metabolic process. IEP. Enrichment. BP. GO:0006139. nucleobase-containing compound metabolic process. IEP. ...

Role Of The Unstructured N-Terminal Domain Of The Human Apurinic/Apyrimidinic Endonuclease 1 (hape1) In The Modulation Of Its ... Interaction With Nucleic Acids And Nucleophosmin (npm1) (504 views). Biochem J (ISSN: 0264-6021, 1470-8728electronic, 0264- ...

Role Of The Unstructured N-Terminal Domain Of The Human Apurinic/Apyrimidinic Endonuclease 1 (hape1) In The Modulation Of Its ... Nucleic Acids Res (ISSN: 1362-4962, 0305-1048, 1362-4962electronic), 2010 Dec; 38(22): 8239-8256.. Impact Factor: 7.836. View ... Nucleic Acids Res (ISSN: 0305-1048, 1362-4962, 1362-4962electronic), 2002 Nov 15; 30(22): 4945-4951.. Impact Factor: 7.051. ... Apurinic/apyrimidinic endonuclease 1 (APE1) is a multifunctional protein contributing to genome stability via repair of DNA ...

In conclusion, GF may ameliorate RA by regulating the composition of intestinal microbiota, α-linolenic acid metabolism, and ... treatment result in synthetic lethality with MMR deficiency through increased cellular apurinic/apyrimidinic (AP) sites, DNA ... In these aggressive resistant ALL cells, POLB depletion and its inhibitor oleanolic acid (OA) ... Pathways involved in metabolomics were mainly α-linolenic acid metabolism and glycerophospholipid metabolism. The results of ...

Amino Acid Sequence, DNA-(Apurinic or Apyrimidinic Site) Lyase, Humans, Models, Molecular, Molecular Sequence Data, Nuclear ...

A/G-specific adenine glycosylase or DNA-(apurinic or apyrimidinic site) lyase (RefSeq). 37, 314. ... exported N-acetylmuramic acid deacetylase (RefSeq). 66, 293. BSU08540. yfhI. putative efflux transporter (RefSeq). 67, 293. ...

Nucleic Acids Research, 37(15):4944-54.. Arora, P., Goyal, A., Natarajan, V. T., Rajakumara, E., Verma, P., Gupta, R., Yousuf, ... Puri, R. V., Singh, N., Gupta, R. K., & Tyagi, A. K. (2013). Endonuclease IV Is the major apurinic/apyrimidinic endonuclease in ... Nucleic Acids Res. Doi:10.1093/nar/gkp502.. Pooja Arora, Aneesh Goyal, Vivek T. Natarajan, Eerappa Rajakumara, Priyanka Verma, ... Arya, R., Dhembla, C., Makde, R. D., Sundd, M., & Kundu, S. (2021). An overview of the fatty acid biosynthesis in the protozoan ...

Has endonucleolytic activity near apurinic sites on DNA.. Links to other databases:. BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS ... Richardson, C.C., Lehman, I.R. and Kornberg, A. A deoxyribonucleic acid phosphatase-exonuclease from Escherichia coli. II. ... Richardson, C.C. and Kornberg, A. A deoxyribonucleic acid phosphatase-exonuclease from Escherichia coli. I. Purification of the ...

Nucleic Acids Research 42:D304-309. doi: 10.1093/nar/gkt1240. Chandonia JM, Guan L, Lin S, Yu C, Fox NK, Brenner SE. 2022. ... PDB Description: apurinic apyrimidinic (ap) endonuclease (nape) from neisseria meningitidis. PDB Compounds: (A:) ... Nucleic Acids Research 50:D553-559. doi: 10.1093/nar/gkab1054. (citing information) Copyright © 1994-2023 The SCOP and SCOPe ...

Nucleic Acids Res. 33: 3779-3784.. * Ji C., RouzerC.A., MarnettL.J., PietenpolJ.A. (1998) Induction of cell cycle arrest by the ... 2001) Two divalent metal ions in the active site of a new crystal form of human apurinic/apyrimidinic endonuclease, Ape1: ... Nucleic Acids Res. 30: e7.. * Werner C., KrebsB., Keith G., Dirheimer G. (1976) Specific cleavages of pure tRNAs by plumbous ... Nucleic Acids Res. 26: 5655-5661.. * Ouyang H., VogelH.J. (1998) Metal ion binding to calmodulin: NMR and fluorescence studies ...

DPA (dipicolinic acid; pyridine-2,6-dicarboxylic acid) is a major component of the spore core, and confers heat resistance upon ... In contrast, no apurinic/apyrimidinic sites were detected in any of our DNA preparations as revealed by digestion with Endo IV ... iv) In the spore core, high levels of pyridine-2,6-dicarboxylic acid (dipicolinic acid, or DPA) enhance spore survival to solar ... To detect apurinic/apyrimidinic (AP) sites, DNA was first digested with Endonuclease IV (Endo IV) (Epicentre Technologies) ( ...

Catalysis of the cleavage of the C-O-P bond 3 to the apurinic or apyrimidinic site in DNA by a beta-elimination reaction, ... Catalysis of the hydrolysis of ester linkages within nucleic acids by creating internal breaks. ...

... α-hydroxycarboxylic acid, catechol and hydroxamic acid) possess two oxygen atoms which coordinate ferric iron in a bidentate ... including strand breaks and apurinic-apyrimidinic (AP) sites (reviewed in Setlow, 2006; Moeller et al., 2007). AP lesions are ... Bacillus subtilis spores saturate their DNA with α/β-type small, acid-soluble spore proteins Selleckchem LDK378 (SASP) to ... that the cell surface-displayed phytase was as least as effective as the secreted phytase in hydrolyzation of phytic acid under ...

The acid polymerase (PA) of influenza viruses is a promising target for new antivirals because of its essential role in ... Apurinic/apyrimidinic endonuclease 1 (APE1), a ubiquitous multipurpose protein, is also known as redox effector factor-1 (Ref-1 ... In this study, we found that cytoplasmic APE1/Ref-1 expression was upregulated in reactive astrocytes of the kainic acid- or ... RO-7 specifically inhibited the ability of the PA endonuclease domain to cleave a nucleic acid substrate. RO-7 also inhibited ...

4-dihydroxyphenylacetic acid; PCA; protocatechuic acid (3,4-dihydroxybenzoic acid); HPA; hippuric acid; DPPH; 1,1-diphenyl-2- ... Keywords: عنصر پاسخ آنتی اکسیدان; AH; antioxidant; APAF-1; apoptotic protease activating factor 1; APE1; apurinic/apyrimidinic ... Nitro-oleic acid; NO2-AA; Nitro-arachidonic acid; Nrf2; Nuclear factor-erythroid 2-related factor 2; ARE; Antioxidant Response ... Electrophilic nitro-fatty acids prevent astrocyte-mediated toxicity to motor neurons in a cell model of familial amyotrophic ...

vi) Development of a sensitive assay for the measurement of apurinic sites in DNA. (vii) Further development of post-labelling ... and ethylating agents were used to determine the nature and the frequency of different types of deoxyribonucleic acid (DNA) ...

The activation of Cr(VI) by intracellular reductants such as glutathione (GSH), ascorbic acid (AsA) and cysteine (Cys), among ... and apurinic/apyrimidinic sites (AP-sites). The potential sites of interaction of damaging species with the DNA macromolecule ...

Betulinic acid is a natural pentacyclic triterpenoid, acts as a eukaryotic topoisomerase I inhibitor, with an IC50 of 5 μM, and ... E3330 (APX-3330) is a direct, orally active and selective inhibitor of Ape-1 (apurinic/apyrimidinic endonuclease 1)/Ref-1 ( ...

In addition, all metal ions tested, with the exception of Mg2+, promote a change in the side-chain position of aspartic acid ... Bennett RA, Wilson DM 3rd, Wong D, Demple B. Interaction of human apurinic endonuclease and DNA polymerase beta in the base ...