... which is a light-up aptamer. Here, we propose to organize this aptamer into a split-aptamer system and determine the limit of ... Previous work by our group has been done to optimize the signal of the dapoxyl-binding aptamer (DAP), ... Aptamers have shown promising ability for detection in the subnanomolar range of nucleic acid targets with specificity down to ... and cost-effectiveness make these nucleic acid aptamers optimal bio-sensors. ...
1DB6: SOLUTION STRUCTURE OF THE DNA APTAMER 5-CGACCAACGTGTCGCCTGGTCG-3 COMPLEXED WITH ARGININAMIDE. ...
SELEX is a well known selection method for fabrication and selection of nucleotide aptamers. SELEX is relatively limited by the ... When the probe surface neighboring an aptamer is blocked by an adjacent aptamer, the redox tag on the target-bound aptamer will ... The concentration of aptamer in solution that incubates a clean probe is found to be proportional to the density of aptamers ... In this method, libraries of aptamers are separated into aptamer particles and separated by FACS based on affinity. Only the ...
Animals; Aptamers, Nucleotide; Cell Line; Cricetinae; Foot-and-Mouth Disease Virus; Gene Expression Regulation, Viral; Genetic ... The 3D(pol) aptamers appeared to be more effective than 2-C-methylcytidine (2CMC). Aptamers to components of the replication ... 3 more authors) (2014) Inhibition of the foot-and-mouth disease virus subgenomic replicon by RNA aptamers. Journal of General ... We have previously documented the inhibitory activity of RNA aptamers to the RNA-dependent RNA polymerase of foot-and-mouth ...
Aptamer library SELEX performance analysis algorithm based on percent GC nucleotide families ... Once an aptamer has been developed we also offer a service to optimise the performance of the aptamer on a range of assay ... Session 3: Aptamers as diagnostic tools. Chair Professor Said Ismail. 16.00: Dr David Bunka, The Aptamer Group, United Kingdom ... 2 Aptamer Group Limited, Bio Centre, Innovation Way, Heslington, York, UK. Abstract: Cell-based aptamer selection is an ...
Among the list of the artificial nucleotides, bridged nucleic acids (BNAs) appear to be the most promising ... Among the list of the artificial nucleotides, bridged nucleic acids (BNAs) appear to be the most promising new generation BNAs ... For the possible applications, BNAs can be used for antisense, antigene, aptamer development, and real-time clamp technology. ... There has been a growing interest in developing chemically modified nucleotides for diagnostics or therapeutics. ...
Keywords: Odds Ratio, Aptamers, Nucleotide, Acute Coronary Syndrome, Myocardial Infarction, Factor IXa, Cardiac Catheterization ...
Specific topics to be covered include aptamers, protein and RNA-based switches and sensors, unnatural amino acids and ... nucleotides, enzyme engineering, and the integration of these parts via synthetic biology efforts. Two lectures. ...
Aptamers, Nucleotide (MeSH) * Base Sequence (MeSH) * Biosensing Techniques (MeSH) * Fluorescence (MeSH) * Fluorescent Dyes ( ... A General Approach to the Construction of Structure-Switching Reporters from RNA Aptamers Academic Article ...
... and Nucleotide-binding oligomerization domain, Leucine rich Repeat and Pyrin domain containing proteins 1 and 3 (NALP1 and ... Oligonucleotide aptamers are well known for their stability and flexibility in conjugating various inflammatory molecules. In ... Beneficial Effects of Inflammatory Cytokine-Targeting Aptamers in an Animal Model of Chronic Prostatitis.. ... Beneficial Effects of Inflammatory Cytokine-Targeting Aptamers in an Animal Model of Chronic Prostatitis. ...
Typical length of ribonucleic acid aptamer Unspecified. 30-70. nucleotides. 105440. Rimmele M. Nucleic acid.... ... Number of different aptamer oligonucleotide sequences in first round of SELEX Unspecified. 10^15. Unitless. 105439. Rimmele M. ... Relevant examples of aptamer molecular beacons application targets, signal recorded and concentration range Unspecified. Table ...
Binding of the each modeled aptamer to the RBD was studied in terms of interactions between residues in protein and nucleotides ... Thus, a new aptamer Apt25 was designed. The procedure of 1) aptamer structure modeling/modification, 2) molecular docking, 3) ... Based on molecular docking results, the only one aptamer (Apt16) was selected from the library as a starting aptamer to the RBD ... aptamer receptor-binding domain SARS-CoV-2 selection oligonukleotidit kvanttikemia laskennallinen kemia koronavirukset ...
O-methylation for all C and U nucleotides). The PSMA aptamer served as a nonspecific control for the OPN aptamer when ... The OPN aptamer, 4-1BB aptamer, and 4-1BB-OPN bispecific aptamers for in vivo study have been previously described (43). ... 4-1BB aptamer (27 days), OPN aptamer (26 days), or a mixture of the 4-1BB and OPN aptamers (23 days) (n = 5 mice/group) (P , ... 4-1BB aptamer and OPN mRNA probe preparation for ISH. In order to make a DNA template for the 4-1BB aptamer, a T7 RNA ...
Here, a new type of stochastic sensor based on an αHL pore modified with an aptamer is described. The aptamer is bound to the ... Here, a new type of stochastic sensor based on an αHL pore modified with an aptamer is described. The aptamer is bound to the ... Here, a new type of stochastic sensor based on an αHL pore modified with an aptamer is described. The aptamer is bound to the ... Here, a new type of stochastic sensor based on an αHL pore modified with an aptamer is described. The aptamer is bound to the ...
Nucleotide Aptamers Medicine & Life Sciences 100% * RNA Probes Medicine & Life Sciences 96% ... Validation of RNA Aptamer Probes to Image Candida albicans in Paraffin-Embedded Sections of Wistar Rat Tongue. In: European ... Validation of RNA Aptamer Probes to Image Candida albicans in Paraffin-Embedded Sections of Wistar Rat Tongue. / Bachtiar, Boy ... Validation of RNA Aptamer Probes to Image Candida albicans in Paraffin-Embedded Sections of Wistar Rat Tongue. European Journal ...
The assay is many times more sensitive than conventional UV absorbance, and free nucleotides or short oligonucleotides of up to ... OliGreen reagent assays are routinely used for antisense oligonucleotides, aptamers, genomic DNA isolated under denaturing ... RiboGreen reagent does not detect significant sample contamination by free nucleotides and thus more accurately measures the ...
Here we used an aptamer displacement screen to identify SecinH3, a small molecule antagonist of cytohesins. The cytohesins are ... They are activated by guanine nucleotide exchange factors (GEFs), which facilitate the exchange of GDP for GTP1,2. This ... Controlling small guanine-nucleotide-exchange factor function through cytoplasmic RNA intramers. Proc. Natl Acad. Sci. USA 98, ... Jackson, C. L. & Casanova, J. E. Turning on ARF: the Sec7 family of guanine-nucleotide-exchange factors. Trends Cell Biol. 10, ...
Thrombotic disorders and their common clinical phenotypes of acute myocardial infarction, ischemic stroke, and venous thromboembolism are the proximate cause of substantial morbidity, mortality, and health care expenditures worldwide. Accordingly, therapies designed to attenuate thrombus initiation and propagation, reflecting integrated platelet-mediated and coagulation protease-mediated events, respectively, represent a standard of care. Unfortunately, there are numerous inherent limitations of existing therapies that include target nonselectivity, variable onset and offset of pharmacodynamic effects, a narrow efficacy-safety profile, and the absence of a safe and reliable platform for either accurate titration, based on existing patient-specific, disease-specific, and clinical conditions, or active reversibility. Herein, we summarize our experience with oligonucleotide antithrombotic agents and their complementary antidotes, targeting the platelet adhesive protein von Willebrand factor and the ...
Aptamers, Nucleotide (MeSH) * Organic Chemistry (Science Metrix) published in * ChemBioChem Journal Research. keywords * ... and aptamers. Research within this space has not only revealed the hidden talents of FNAs but has also laid important ... including naturally occurring ribozymes and riboswitches as well as artificially created DNAzymes and aptamers, have been ...
Keywords: Alzheimers disease, biomarkers, blood, magnetic resonance imaging, nucleotide aptamers, proteome DOI: 10.3233/JAD- ...
Nucleotide Aptamers 17% * Virus Latency 16% * Messenger RNA 13% Agriculture & Biology. * nuclear antigens 91% ...
93del is a 16-nucleotide G-quadruplex-forming aptamer which can inhibit the activity of the HIV-1 integrase enzyme at nanomolar ... Crystal structures of an HIV-1 integrase aptamer: formation of a water-mediated A•G•G•G•G pentad in an interlocked G-quadruplex ... Ngo, K. H., Liew, C. W., Lattmann, S., Winnerdy, F. R. & Phan, A. T. (2022). Crystal structures of an HIV-1 integrase aptamer: ... To gain further insights on the structure of this unique aptamer, we have determined several high-resolution crystal structures ...
Generative aptamer discovery using RaptGen. Iwano, N., Adachi, T., Aoki, K., Nakamura, Y. & Hamada, M., 2022 6月, In: Nature ... Prediction of RNA-protein interactions using a nucleotide language model. Yamada, K. & Hamada, M., 2022, In: Bioinformatics ... RaptRanker: In silico RNA aptamer selection from HT-SELEX experiment based on local sequence and structure information. Ishida ...
In vivo SELEX strategies to identify potent aptamer-drug conjugates for glioblastoma. Sarkaria, J. N. & Maher, L. L. J. ... Nucleotide Aptamers 37% * Pharmaceutical Preparations 20% * Venous Thrombosis After Traumatic Injury. Park, M. S. & Auton, M. T ...
Modified aptamers may show improved chemical diversity relative to aptamers composed entirely of natural DNA or RNA nucleotides ... Latham, A.J.; Johnson, R.; Toole, J.J. The application of a modified nucleotide in aptamer selection: Novel thrombin aptamers ... Circular bivalent aptamers (cb aptamers) were constructed from aptamers selected against live cancer cells, and were tested for ... Ligand-guided selection (LIGS) of aptamers is known to give aptamers with high specificity; however, these aptamers suffer from ...
Nucleotide Aptamers Medicine & Life Sciences 100% * CTLA-4 Antigen Medicine & Life Sciences 95% ... Huang, BT, Lai, WY, Chang, YC, Wang, JW, Yeh, SD, Lin, EPY & Yang, PC 2017, A CTLA-4 Antagonizing DNA Aptamer with Antitumor ... A CTLA-4 Antagonizing DNA Aptamer with Antitumor Effect. Bo Tsang Huang, Wei Yun Lai, Yi Chung Chang, Jen Wei Wang, Shauh Der ... A CTLA-4 Antagonizing DNA Aptamer with Antitumor Effect. / Huang, Bo Tsang; Lai, Wei Yun; Chang, Yi Chung et al. In: Molecular ...
Unnatural Nucleotides: Exploring the Information Storage System of DNA. PubMed, SCI, Scopus, ESCI, PMC indexed ... 3.2 DNA Aptamers using UBPs. DNA Aptamers are in-vitro generated via an evolutional engineering method known as SELEX, ( ... Percze K, Mészáros T. Analysis of Modified Nucleotide Aptamer Library Generated by Thermophilic DNA Polymerases, ChemBioChem 21 ... Ligand-Guided Selection of Target-Specific Aptamers: A Screening Technology for Identifying Specific Aptamers Against Cell- ...
Nucleotide Aptamers Medicine & Life Sciences 39% * RNA-Binding Motifs Medicine & Life Sciences 37% ... However, more effort is needed to identify and characterize the sequence-structure motifs in the aptamers important for binding ... However, more effort is needed to identify and characterize the sequence-structure motifs in the aptamers important for binding ... However, more effort is needed to identify and characterize the sequence-structure motifs in the aptamers important for binding ...
... an aptamer, an antibody, a antisense nucleotide, a siRNA, a shRNA, and a miRNA that specifically bind to TINAGL1 protein or a ... The agent may be selected from the group consisting of a primer, a probe, an antibody, a peptide, and an aptamer. ... The TINAGL1 inhibitor may be any one selected from the group consisting of a peptide, an aptamer, an antibody, a antisense ... nucleotide, a siRNA, a shRNA, and a miRNA that specifically bind to the TINAGL1 protein or a gene encoding the protein. ...