*  51Cr Release Assay of AntibodyDependent Cell‐Mediated Cytotoxicity (ADCC) - Current Protocols
... dependent cell‐mediated cytotoxicity (ADCC) is an immunologic cytotoxic effector mechanism that is dependent on the cooperative ... Antibodydependent cell‐mediated cytotoxicity (ADCC) is an immunologic cytotoxic effector mechanism that is dependent on the ... 51Cr Release Assay of AntibodyDependent Cell‐Mediated Cytotoxicity (ADCC). David L. Nelson1, Carole C. Kurman1, Deborah E. ... Antibody‐coated and non‐antibody‐coated 51Cr‐labeled target‐cell (e.g., Chang‐cell) suspensions in complete RPMI‐10 medium (1 ...
  http://www.currentprotocols.com/WileyCDA/CPUnit/refId-im0727.html
*  Plus it
We also measured the antibody-dependent cellular cytotoxicity (ADCC) activity in neutrophils and studied the expression of cell ... In brief, target cells (chicken red blood cells) were labeled with 51Cr (100 μCi per 106 cells) at 37°C for 1 hour. Cells were ... Cells were then sensitized with antibodies (final concentration, 5 μg/mL). The neutrophils of a patient and target cells were ... G-CSF administration increased both Fc γ receptor type I expression on neutrophils and antibody-dependent cellular cytotoxicity ...
  http://clincancerres.aacrjournals.org/content/11/2/697
*  Anti tumour Monoclonal antibodies | Master Of Medicine
Monoclonal antibodies against tumour cells mainly act via antibody dependent cellular cytotoxicity. ... Anti tumour Monoclonal antibodies. Posted by admin on July 19, 2011 Leave a comment (0) Go to comments ...
  http://masterofmedicine.com/anti-tumour-monoclonal-antibodies/
*  Assessment of New Developments in ADCC Assays | SelectScience
... assays play an integral part in the antibody therapeutic drug discovery process. New, non-radioactive assay chemistries are ... Antibody-dependent cell-mediated cytotoxicity (ADCC) assays play an integral part in the antibody therapeutic drug discovery ...
  http://www.selectscience.net/application-articles/assessment-of-new-developments-in-adcc-assays/?artID=27360&compID=1191
*  Paper: Polatuzumab Vedotin Combined with Obinutuzumab, Cyclophosphamide, Doxorubicin, and Prednisone (G-CHP) for Patients with...
... type II anti-CD20 monoclonal antibody with improved direct cell death and antibody-dependent cell-mediated cytotoxicity ... See more of: Aggressive Lymphoma (Diffuse Large B-Cell and Other Aggressive B-Cell Non-Hodgkin Lymphomas)-Results from ... Aggressive Lymphoma (Diffuse Large B-Cell and Other Aggressive B-Cell Non-Hodgkin Lymphomas)-Results from Prospective Clinical ... Aggressive Lymphoma (Diffuse Large B-Cell and Other Aggressive B-Cell Non-Hodgkin Lymphomas)-Results from Prospective Clinical ...
  https://ash.confex.com/ash/2017/webprogram/Paper103044.html
*  Abstract 1332: Interleukin-15 enhances rituximab-dependent cytotoxicity ex vivo and in vivo against a mouse lymphoma expressing...
Rituximab (RTX), an anti-CD20 antibody, revolutionized treatment for B-cell malignancies, but it is not without its own ... IL-15 enhances antibody-dependent cellular cytotoxicity (ADCC), an important mechanism of RTX, by increasing the proliferation ... NK cells were cultured in triplicate with 51Cr-labeled EL4-CD20 cells with or without RTX and lysis was evaluated using a ... Interleukin-15 enhances rituximab-dependent cytotoxicity ex vivo and in vivo against a mouse lymphoma expressing human CD20. [ ...
  http://cancerres.aacrjournals.org/content/75/15_Supplement/1332
*  CBT Pharmaceuticals Presents Data Demonstrating Anti-Tumor Activity of its Programmed Death-1 antibody, CBT-501, at ASCO-SITC...
... anti-PD-1 antibodies and has a superior safety profile with very low undesirable antibody-dependent cell-mediated cytotoxicity ... and complement-dependent cytotoxicity (CDC) activity. The antibody (GB226) has been developed by Genor BioPharma Co. Ltd., a ... antibody that restores the body's immune system to recognize and kill cancer cells. CBT is headquartered in California and has ... antibody, CBT-501 (genolimzumab, GB-226), in stimulating various immune cells, generating anti-tumor immunity, and suppressing ...
  https://www.cbtpharma.com/media/cbt-pharmaceuticals-presents-data-demonstrating-anti-tumor-activity-programmed-death-1-antibody-cbt-501-asco-sitc-clinical-oncology-symposium/
*  Influence of the Envelope gp120 Phe 43 Cavity on HIV-1 Sensitivity to Antibody-Dependent Cell-Mediated Cytotoxicity Responses. ...
Home » Influence of the Envelope gp120 Phe 43 Cavity on HIV-1 Sensitivity to Antibody-Dependent Cell-Mediated Cytotoxicity ... Influence of the Envelope gp120 Phe 43 Cavity on HIV-1 Sensitivity to Antibody-Dependent Cell-Mediated Cytotoxicity Responses. ... Influence of the Envelope gp120 Phe 43 Cavity on HIV-1 Sensitivity to Antibody-Dependent Cell-Mediated Cytotoxicity Responses. ... in the CD4-bound conformation on their surface are preferentially targeted by antibody-dependent cellular-mediated cytotoxicity ...
  http://chavi-id.org/publications/influence-envelope-gp120-phe-43-cavity-hiv-1-sensitivity-antibody-dependent-cell
*  Antibody-dependent cell-mediated cytotoxicity - Wikipedia
The antibody-dependent cell-mediated cytotoxicity (ADCC), also referred to as antibody-dependent cellular cytotoxicity, is a ... "Antibody-dependent cell-mediated cytotoxicity against influenza virus-infected cells". The Journal of Infectious Diseases. 148 ... Wang, W; Erbe, AK; Hank, JA; Morris, ZS; Sondel, PM (2015). "NK Cell-Mediated Antibody-Dependent Cellular Cytotoxicity in ... ISBN 1-55581-246-5. University of Leicester, Virus Immunopathology Notes Antibody-Dependent Cell Cytotoxicity at the US ...
  https://en.wikipedia.org/wiki/Antibody-dependent_cell-mediated_cytotoxicity
*  Antibody-Dependent Cell Cytotoxicities - Medical Dictionary online-medical-dictionary.org
Antibody-Dependent Cell Cytotoxicities. The phenomenon of antibody-mediated target Cell destruction by non-sensitized effector ... The effector Cell is a "killer" Cell possessing Fc Receptors. It may be a Lymphocyte lacking conventional B- or T-Cell markers ... The identity of the target Cell varies, but it must possess surface Immunoglobulin G whose Fc portion is intact. ... or a Monocyte, Macrophage, or polynuclear Leukocyte, depending on the identity of the target Cell. The reaction is Complement- ...
  http://www.online-medical-dictionary.org/definitions-a/antibody-dependent-cell-cytotoxicities.html
*  Heterogeneous neutralizing antibody and antibody-dependent cell cytotoxicity responses in HIV-1 elite controllers
... ... antibody-dependent cell cytotoxicity (ADCC), as well as autoantibody levels, were quantified in plasma from 22 controllers and ... Regardless of the type of antibody tested, there was no correlation with HIV-specific CD8 T cell responses. ADCC was detectable ... The levels of these different antibody responses and HIV-specific CD8 T cell responses quantified by enzyme-linked ...
  https://insights.ovid.com/crossref?an=00002030-200905150-00004
*  The Use of Elplasia™ Microplates for Antibody-Dependent Cell Mediated Cytotoxicity (ADCC) Applications | SelectScience
Traditional pharmacological studies done in cell monolayers have limited translational value; because, they do not capture the ... The need for biologically relevant assays with clinical translatability has brought the 3D cell culture model to the forefront ... Application Note: The Use of Elplasia™ Microplates for Antibody-Dependent Cell Mediated Cytotoxicity (ADCC) Applications. 6 ... the suitability of Kuraray's Elplasia Square Type plates for antibody-dependent cell mediated cytotoxicity assays, using 3D ...
  https://www.selectscience.net/application-articles/the-use-of-elplasia--microplates-for-antibody-dependent-cell-mediated-cytotoxicity-
*  MEDI-563, a humanized anti-IL-5 receptor alpha mAb with enhanced antibody-dependent cell-mediated cytotoxicity function
... and it inhibits IL-5-mediated cell proliferation. MEDI-563 potently induces antibody-dependent cell-mediated cytotoxicity of ... and it inhibits IL-5-mediated cell proliferation. MEDI-563 potently induces antibody-dependent cell-mediated cytotoxicity of ... Primary human eosinophils and basophils were used to demonstrate antibody-dependent cell-mediated cytotoxicity. The binding ... CONCLUSIONS: MEDI-563 might provide a novel approach for the treatment of asthma through active antibody-dependent cell- ...
  https://www.garvan.org.au/research/publications/10750
*  The participation of antibody in spontaneous and antibody-dependent cell-mediated cytotoxicity in the pig | IslandScholar
The participation of antibody in spontaneous and antibody-dependent cell-mediated cytotoxicity in the pig. Primary tabs. *View( ... The participation of antibody in spontaneous and antibody-dependent cell-mediated cytotoxicity in the pig ... PK15-TGE cells), but had no effect on antibody-dependent cell-mediated cytotoxicity (ADCC). SCMC activity was partially ... PK15-TGE cells), but had no effect on antibody-dependent cell-mediated cytotoxicity (ADCC). SCMC activity was partially ...
  http://www.islandscholar.ca/islandora/object/ir:ir-batch6-3274
*  Possible Evidence for Antibody-Dependent Macrophage-Mediated Cytotoxicity Directed against Murine Adenocarcinoma Cells in Vivo ...
Possible Evidence for Antibody-Dependent Macrophage-Mediated Cytotoxicity Directed against Murine Adenocarcinoma Cells in Vivo ... Possible Evidence for Antibody-Dependent Macrophage-Mediated Cytotoxicity Directed against Murine Adenocarcinoma Cells in Vivo ... Possible Evidence for Antibody-Dependent Macrophage-Mediated Cytotoxicity Directed against Murine Adenocarcinoma Cells in Vivo ... Possible Evidence for Antibody-Dependent Macrophage-Mediated Cytotoxicity Directed against Murine Adenocarcinoma Cells in Vivo ...
  http://www.jimmunol.org/content/117/5_Part_2/1992
*  Interaction with cellular CD4 exposes HIV-1 envelope epitopes targeted by antibody-dependent cell-mediated cytotoxicity. |...
Interaction with cellular CD4 exposes HIV-1 envelope epitopes targeted by antibody-dependent cell-mediated cytotoxicity.'s ... such as antibody-dependent cell-mediated cytotoxicity (ADCC). Env epitopes targeted by many antibodies effective at mediating ... 1 envelope epitopes targeted by many antibodies effective at mediating antibody-dependent cell-mediated cytotoxicity (ADCC) are ... Interaction with cellular CD4 exposes HIV-1 envelope epitopes targeted by antibody-dependent cell-mediated cytotoxicity. ...
  https://scholars.duke.edu/display/pub1003231
*  Phase II trial of the anti-G(D2) ... preview & related info | Mendeley
PURPOSE: To describe oncolytic effects of treatment with anti-G(D2) monoclonal antibody 3F8 plus granulocyte-macrophage colony- ... Antibody-Dependent Cell Cytotoxicity. *Bone Marrow Neoplasms. *Bone Marrow Neoplasms: drug therapy ... Phase II trial of the anti-G(D2) monoclonal antibody 3F8 and granulocyte-macrophage colony-stimulating factor for neuroblastoma ... PURPOSE: To describe oncolytic effects of treatment with anti-G(D2) monoclonal antibody 3F8 plus granulocyte-macrophage colony- ...
  https://www.mendeley.com/research-papers/phase-ii-trial-antigd2-monoclonal-antibody-3f8-granulocytemacrophage-colonystimulating-factor-neurob/
*  Frontiers | Natural Killer Cell Immunomodulation: Targeting Activating, Inhibitory, and Co-stimulatory Receptor Signaling for...
NK cells directly identify and lyse cancer cells. Nascent transformed cells elicit NK cell activation and are eliminated. ... NK cells directly identify and lyse cancer cells. Nascent transformed cells elicit NK cell activation and are eliminated. ... but recent advances in elucidating NK cell biology have accelerated the development of NK cell-targeting therapeutics. NK cell ... but recent advances in elucidating NK cell biology have accelerated the development of NK cell-targeting therapeutics. NK cell ...
  https://www.frontiersin.org/articles/10.3389/fimmu.2015.00601/full
*  To Evaluate Antimicrobial Properties of Platelet Rich Plasma and Source of Colonization in Pressure Ulcers in Spinal Injury...
Platelets participate in antibody-dependent cell cytotoxicity functions to kill protozoal pathogens. Finally, platelets release ... The red blood cells layer was at the lowest level because of different sediment coefficients. The platelet layer was in the ... Red cells layer was drawn from the tube. The remainder was agitated for several seconds and underwent a second centrifugation ... Another limitation was that we did not perform cell, bacterial, and factor estimation in the study. Future research directions ...
  https://www.hindawi.com/journals/ulcers/2015/749585/
*  Development of a Human Monoclonal Antibody for Potential Therapy of CD27-Expressing Lymphoma and Leukemia | Clinical Cancer...
Antibody-dependent cell-mediated cytotoxicity. Target cells (Ramos and Daudi cells) were loaded with Calcein AM (Life ... cells, CD4+ cells, CD8+ cells, B cells (CD20+), memory B cells (CD20+, CD95+), naive B cells (CD20+, CD95−), NK cells (CD3−, ... The anti-CD27 mAb 1F5 was effective in mediating antibody-dependent cellular cytotoxicity of human lymphoblastic cell lines and ... Cells were washed and analyzed. Subset markers include CD3+ T cells, CD3+CD4+; CD8+ T cells, CD3+CD8+; B cells, CD19+CD20+; ...
  http://clincancerres.aacrjournals.org/content/18/14/3812
*  Plus it
Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC).. To evaluate ADCC effects mediated by CA125-specifc Ab3 antibodies, ... Additionally, the development of CA125-specific antibodies (Ab1′) and antibody-dependent cell-mediated cytotoxicity of CA125- ... Median survival with respect to immune response (human antimouse antibody, Ab3, Ab1′, and antibody-dependent cell-mediated ... A total of 2.5 × 105 peripheral blood mononuclear cells/well were added to the target cells, resulting in an E:T cell ratio of ...
  http://clincancerres.aacrjournals.org/content/10/5/1580
*  Distinct Apoptotic Signaling Characteristics of the Anti-CD40 Monoclonal Antibody Dacetuzumab and Rituximab Produce Enhanced...
Antibody-dependent cell mediated-cytotoxicity and apoptosis assays. ADCC was measured using a standard 51Cr release assay. ... dacetuzumab is an IgG1 antibody capable of mediating antibody-dependent cellular cytotoxicity (ADCC) and antibody-dependent ... Apoptotic signaling, not antibody-dependent cell mediated-cytotoxicity, is enhanced in the dacetuzumab-rituximab combination. ... and it is able to mediate antibody dependent cellular cytotoxicity (ADCC), complement dependent cytotoxicity (CDC), and ...
  http://clincancerres.aacrjournals.org/content/17/14/4672
*  Molecular Pathways to Polyglutamine Aggregation | SpringerLink
The structural impact of a polyglutamine tract is location-dependent. Biophys J 2008; 95(12):5922-5930.PubMedCrossRefGoogle ... Cleavage of atrophin-1 at caspase site aspartic acid 109 modulates cytotoxicity. J Biol Chem 1999; 274(13):8730-8736.PubMed ... Monoclonal antibodies recognize distinct conformational epitopes formed by polyglutamine in a mutant huntingtin fragment. J ... Inhibition of polyglutamine protein aggregation and cell death by novel peptides identified by phage display screening. J Biol ...
  https://link.springer.com/chapter/10.1007/978-1-4614-5434-2_7
*  Tet-On 3G Tetracycline Inducible Expression Systems - EF1-Alpha Promoter
Human Stem Cell Markers * Hepatic Cell Differentiation Antibodies * Pluripotency and Differentiation * iPS Cell to Hepatocyte ... Methylation Dependent Restriction Enzyme * Cytosine Methylation Modification Enzyme * DNA Methylation * Methylated DNA ... Cell Proliferation and Viability * Actin Dynamics * Heme Oxygenase-1 * LDH Cytotoxicity * WST-1 Cell Proliferation ... Inducible expression in cell types known to silence CMV promoters, such as hematopoietic cells and stem cells ...
  http://www.clontech.com/US/Products/Inducible_Systems/Tetracycline-Inducible_Expression/Tet-On_3G_EF1-Alpha_Promoter?sitex=10020:22372:US&PROD=GEek1d-0UkeWYwXRAPsCISCe:S&PROD_pses=ZG45559CA3B22DF300C8BFC0BD95D52D46D035AECC223FA91B3473EC5591AFFCB9D934A327070F2CB4FC0E69895B11889D3B72188AFA49C6E9
*  Bicistronic IRES Tet-On 3G Tetracycline Inducible Expression Systems
Human Stem Cell Markers * Hepatic Cell Differentiation Antibodies * Pluripotency and Differentiation * iPS Cell to Hepatocyte ... Methylation Dependent Restriction Enzyme * Cytosine Methylation Modification Enzyme * DNA Methylation * Methylated DNA ... Cell Proliferation and Viability * Actin Dynamics * Heme Oxygenase-1 * LDH Cytotoxicity * WST-1 Cell Proliferation ... Electroporation of Cas9-sgRNA ribonucleoproteins (RNPs) for gene editing in diverse cell types ...
  http://www.clontech.com/US/Products/Inducible_Systems/Tetracycline-Inducible_Expression/Tet-On_3G_IRES_Bicistronic?sitex=10020:22372:US&PROD=uP0fh0WAQbnmA12cbwGU-Bqx:S&PROD_pses=ZG029F5935059F77B0296F28FEE845DB275FBA17C95795C7BAD224817E4FDB25ADFA64AF8CCD1F8600C29606D0FB95A5C9C075F7E1F7D0B605