*  RFA-CA-07-003: Innovations in Cancer Sample Preparation (R21, R33, R21/R33)
Innovations in Cancer Sample Preparation (R21, R33, R21/R33) RFA-CA-07-003. NCI ... Sample preparation methods vary. Researchers often develop sample preparation methodologies in an ad hoc fashion as part of ... whether or not any analytic tools also will be provided, whether or not a data-sharing agreement will be required and, if so, a ... Sample preparation methods and technologies may be developed for sample collection, processing, isolation, storage, ...
  https://grants.nih.gov/grants/guide/rfa-files/RFA-CA-07-003.html
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Analytic Sample Preparation Methods, Clinical Laboratory Techniques, Molecular Diagnostic Techniques, Genetic Techniques, ... This method presented here adapts the method designed previously for rat livers to human liver pieces and results in a large ... The method described here was validated against a method of HIV-1 drug resistance testing approved by the United States Food ... Isolate single bacterial colonies by the streak-plating method. ● Use pour-plating and spread-plating methods to determine the ...
  https://www.jove.com/visualize/abstract/21531321/blood-purification-in-toxicology-nephrologys-ugly-duckling
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Analytic Sample Preparation Methods, Clinical Laboratory Techniques, Molecular Diagnostic Techniques, Genetic Techniques, ... In addition to detailing the methods for sample preparation and data acquisition, we here describe the optical setup for FPALM ... Bi-directional flow maximizes residence time between the monolith and sample, and enables large sample volumes to be processed ... sample preparation, nasopharyngeal aspirate, blood, plasma, high-throughput, sequencing ...
  https://www.jove.com/visualize/abstract/23840621/guillain-barr-syndrome-influenza-vaccination-antecedent-respiratory
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Analytic Sample Preparation Methods, Clinical Laboratory Techniques, Molecular Diagnostic Techniques, Genetic Techniques, ... Bi-directional flow maximizes residence time between the monolith and sample, and enables large sample volumes to be processed ... tissue samples that may yield small amounts of degraded or damaged DNA. Here, we describe the preparation of barcoded and ... sample preparation, nasopharyngeal aspirate, blood, plasma, high-throughput, sequencing ...
  https://www.jove.com/visualize/abstract/21072188/the-impact-case-diagnosis-coverage-diagnosis-delays-on-effectiveness
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Analytic Sample Preparation Methods, Clinical Laboratory Techniques, Molecular Diagnostic Techniques, Genetic Techniques, ... The method then shifts focus to flight preparation, by offering guidelines and suggestions to prepare for a successful flight ... The method described here was validated against a method of HIV-1 drug resistance testing approved by the United States Food ... However, technical variation in sample preparation can significantly affect quality of flow cytometry data. Thus, ...
  https://www.jove.com/visualize/abstract/23505427/exploratory-analysis-methods-for-automated-classification-laboratory
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Analytic Sample Preparation Methods, Clinical Laboratory Techniques, Molecular Diagnostic Techniques, Genetic Techniques, ... Bi-directional flow maximizes residence time between the monolith and sample, and enables large sample volumes to be processed ... through the method of electron tomography4,5. Moreover, OpNS can be a high‐throughput tool to examine hundreds of samples of ... but only double staining could be applied in these samples. We have developed a new flow cytometry-based method7 to analyze the ...
  https://www.jove.com/visualize/abstract/22506016/longer-leukocyte-telomere-length-is-associated-with-smaller
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Chemistry, Issue 79, Chemistry Techniques, Analytical, Mass Spectrometry, Analytic Sample Preparation Methods, biochemistry, ... and the utility of the thin layer method as approach for sample deposition. We also discuss the critical role of the matrix and ... Analysis of intact proteins, mass spectrometry, matrix-assisted laser desorption ionization, time of flight, sample preparation ... such as small brain samples or even single cells. The protocol is most advantageous for parallel analysis of multiple samples ( ...
  https://www.jove.com/visualize/abstract/24358096/correction-enhanced-recognition-memory-after-incidental-encoding
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Analytic Sample Preparation Methods, Clinical Laboratory Techniques, Molecular Diagnostic Techniques, Genetic Techniques, ... In our method, each hair sample is first washed twice in isopropanol to remove any CORT from the outside of the hair shaft that ... The benefit of this vascular casting method as compared to other techniques is its broad adaptation to various analytic tools, ... Bi-directional flow maximizes residence time between the monolith and sample, and enables large sample volumes to be processed ...
  https://www.jove.com/visualize/abstract/25360669/a-case-control-study-maternal-periconceptual-pregnancy-recreational
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Analytic Sample Preparation Methods, Clinical Laboratory Techniques, Molecular Diagnostic Techniques, Genetic Techniques, ... Experimental methods such as directed evolution have shown success in protein design. However, such methods are restricted by ... Bi-directional flow maximizes residence time between the monolith and sample, and enables large sample volumes to be processed ... detection method for high-throughput and multiplexed profile glycosylation of native glycoproteins in a complex sample 18. In ...
  https://www.jove.com/visualize/abstract/22427815/molecular-prognostic-prediction-for-locally-advanced-nasopharyngeal
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Analytic Sample Preparation Methods, Clinical Laboratory Techniques, Molecular Diagnostic Techniques, Genetic Techniques, ... Unlike this latter method, sampling from an arterial catheter is not stressful to the mouse1. We also describe methods for ... This technique provides a significant advantage to the most common method for obtaining blood samples during insulin clamps ... A vital aspect of metabolomics is sample preparation whereby inconsistent techniques generate unreliable results. This ...
  https://www.jove.com/visualize/abstract/21364873/maternal-high-fat-diet-is-associated-with-decreased-plasma-n-3-fatty
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Analytic Sample Preparation Methods, Clinical Laboratory Techniques, Molecular Diagnostic Techniques, Genetic Techniques, ... Bi-directional flow maximizes residence time between the monolith and sample, and enables large sample volumes to be processed ... sample preparation, nasopharyngeal aspirate, blood, plasma, high-throughput, sequencing ... Vaccine samples were applied onto a PVDF membrane using a slot blot apparatus along with reference standards diluted to various ...
  https://www.jove.com/visualize/abstract/20479875/pandemic-influenza-h1n1-2009-is-associated-with-severe-disease
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Analytic Sample Preparation Methods, Clinical Laboratory Techniques, Molecular Diagnostic Techniques, Genetic Techniques, ... Recently, Kraus et al.18 introduced a method to collect, store and transport AIV samples, based on a special filter paper. FTA ... A control method known as RIDL (Release of Insects carrying a Dominant Lethal)4, is based around SIT, but uses genetic methods ... The EP method produces a high percentage of viable embryos that can develop to fertile adults of either sex. The method ...
  https://www.jove.com/visualize/abstract/25386907/transfer-maternal-antibodies-against-avian-influenza-virus-mallards
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Chemistry, Issue 79, Chemistry Techniques, Analytical, Mass Spectrometry, Analytic Sample Preparation Methods, biochemistry, ... A control method known as RIDL (Release of Insects carrying a Dominant Lethal)4, is based around SIT, but uses genetic methods ... and the utility of the thin layer method as approach for sample deposition. We also discuss the critical role of the matrix and ... Analysis of intact proteins, mass spectrometry, matrix-assisted laser desorption ionization, time of flight, sample preparation ...
  https://www.jove.com/visualize/abstract/24324570/correction-intra-trans-generational-costs-reduced-female-body-size
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Chemistry, Issue 79, Chemistry Techniques, Analytical, Mass Spectrometry, Analytic Sample Preparation Methods, biochemistry, ... The assay consists of three parts: The first part involves preparation of the samples for testing, the second part is an ... The method described here was validated against a method of HIV-1 drug resistance testing approved by the United States Food ... Experimental methods such as directed evolution have shown success in protein design. However, such methods are restricted by ...
  https://www.jove.com/visualize/abstract/24324533/correction-cardio-metabolic-effects-hiv-protease-inhibitors
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Analytic Sample Preparation Methods, Clinical Laboratory Techniques, Molecular Diagnostic Techniques, Genetic Techniques, ... Recently, Kraus et al.18 introduced a method to collect, store and transport AIV samples, based on a special filter paper. FTA ... The assay consists of three parts: The first part involves preparation of the samples for testing, the second part is an ... The method described here was validated against a method of HIV-1 drug resistance testing approved by the United States Food ...
  https://www.jove.com/visualize/abstract/20814575/serological-response-rt-pcr-confirmed-h1n1-2009-influenza
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Analytic Sample Preparation Methods, Clinical Laboratory Techniques, Molecular Diagnostic Techniques, Genetic Techniques, ... Bi-directional flow maximizes residence time between the monolith and sample, and enables large sample volumes to be processed ... and can be implemented in conjunction with other staining methods performed on fixed embryo samples. ... sample preparation, nasopharyngeal aspirate, blood, plasma, high-throughput, sequencing ...
  https://www.jove.com/visualize/abstract/25803442/shrimp-serine-proteinase-homologues-pmmassph-1-2-play-role-activation
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Analytic Sample Preparation Methods, Clinical Laboratory Techniques, Molecular Diagnostic Techniques, Genetic Techniques, ... This has led to a variety of sampling and sample processing procedures currently in use. The set of methods presented here ... Finally, this method allows for the observation of the same live leaf sample over extended periods of time using conditions ... The reconstitution method is powerful for various reasons: (1) pure preparations of individual complexes can be obtained, (2) ...
  https://www.jove.com/visualize/abstract/20949036/comparative-genomic-evidence-for-complete-nuclear-pore-complex-last
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Analytic Sample Preparation Methods, Clinical Laboratory Techniques, Molecular Diagnostic Techniques, Genetic Techniques, ... Bi-directional flow maximizes residence time between the monolith and sample, and enables large sample volumes to be processed ... Compared with the lipid transfection method 11,12, this modified calcium phosphate transfection method is cheaper, easier to ... sample preparation, nasopharyngeal aspirate, blood, plasma, high-throughput, sequencing ...
  https://www.jove.com/visualize/abstract/25816015/the-impact-immunosenescence-on-humoral-immune-response-variation
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Chemistry, Issue 79, Chemistry Techniques, Analytical, Mass Spectrometry, Analytic Sample Preparation Methods, biochemistry, ... In addition to detailing the methods for sample preparation and data acquisition, we here describe the optical setup for FPALM ... enzymatic DNA extraction method for fecal samples to enhance the removal of bacteria and DNA from the sample matrix and improve ... The aim was to establish in vivo sampling methods for the use in a bovine model of a respiratory Chlamydia psittaci infection. ...
  https://www.jove.com/visualize/abstract/24348928/correction-genetic-diversity-population-structure-rice-pathogen
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Analytic Sample Preparation Methods, Clinical Laboratory Techniques, Molecular Diagnostic Techniques, Genetic Techniques, ... In addition to detailing the methods for sample preparation and data acquisition, we here describe the optical setup for FPALM ... However, for small tissue samples, this analysis method may not be sufficiently sensitive to detect a protein of interest. To ... The procedure includes guidelines for sample organization and preparation, data acquisition, quality control, and metabolite ...
  https://www.jove.com/visualize/abstract/22761696/normacurve-supercurve-based-method-that-simultaneously-quantifies
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Analytic Sample Preparation Methods, Clinical Laboratory Techniques, Molecular Diagnostic Techniques, Genetic Techniques, ... In addition to detailing the methods for sample preparation and data acquisition, we here describe the optical setup for FPALM ... This has led to a variety of sampling and sample processing procedures currently in use. The set of methods presented here ... enzymatic DNA extraction method for fecal samples to enhance the removal of bacteria and DNA from the sample matrix and improve ...
  https://www.jove.com/visualize/abstract/19924285/a-rapid-economic-house-dna-purification-method-using-glass-syringe
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Chemistry, Issue 79, Chemistry Techniques, Analytical, Mass Spectrometry, Analytic Sample Preparation Methods, biochemistry, ... such as labor intensive sample preparations, low detection limits, slow scan speeds, intensive method optimization for each ... Traditional methods of sampling human saliva involving centrifugation to remove debris 3-4 may be too time-consuming to be ... We present here a simple and fast bacterial pellet preparation from a positive blood culture that can be used as a sample for ...
  https://www.jove.com/visualize/abstract/24312330/identification-monitoring-host-cell-proteins-mass-spectrometry
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Chemistry, Issue 79, Chemistry Techniques, Analytical, Mass Spectrometry, Analytic Sample Preparation Methods, biochemistry, ... and the utility of the thin layer method as approach for sample deposition. We also discuss the critical role of the matrix and ... Analysis of intact proteins, mass spectrometry, matrix-assisted laser desorption ionization, time of flight, sample preparation ... Further, this method can be used to identify novel therapeutic targets at cellular level and to test the effectiveness of new ...
  https://www.jove.com/visualize/abstract/24376481/correction-relationship-cha2ds2-vasc-chads2-score-to-left-atrial
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Analytic Sample Preparation Methods, Clinical Laboratory Techniques, Molecular Diagnostic Techniques, Genetic Techniques, ... Two different methods are described to assess β-catenin protein degradation in Xenopus egg extract. One method is visually ... Here we demonstrate a straightforward method for the induction of EMT in a variety of cell types. Methods for the analysis of ... Bi-directional flow maximizes residence time between the monolith and sample, and enables large sample volumes to be processed ...
  https://www.jove.com/visualize/abstract/24040157/radioactive-i-seed-inhibits-cell-growth-migration-invasion
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Analytic Sample Preparation Methods, Clinical Laboratory Techniques, Molecular Diagnostic Techniques, Genetic Techniques, ... The method described here was validated against a method of HIV-1 drug resistance testing approved by the United States Food ... Bi-directional flow maximizes residence time between the monolith and sample, and enables large sample volumes to be processed ... sample preparation, nasopharyngeal aspirate, blood, plasma, high-throughput, sequencing ...
  https://www.jove.com/visualize/abstract/21179517/hospitalized-children-with-2009-pandemic-influenza-h1n1-comparison-to