Soft-Agar Assays. After transfection and FA treatment, cells were seeded in soft agar growth medium in 6-well plates (5,000 ... FA for 48 h. The cells were then plated in 0.35% soft agar and cultured for 4 wk. The data shown are the mean. ±. standard. ... A) Formaldehyde (FA) facilitates colony formation of BEAS-2B cells on soft agar. After FA treatment (. 100. μ. M. ), the cells ... FA for 24 h or 48 h, plated in soft agar, and cultured for 5 wk. Exposure to FA greatly enhanced colony formation when cells ...

iAs for 7 d. Quantification data of soft-agar colony formation efficiency are mean. ±. SEM. (. n. =. 6. ); *. p. ,. 0.05. ... Soft agar colony growth assays were applied to examine iAs-induced transformation. For in vivo study, PrSPCs mixed with rat ... Soft-Agar Colony Formation Assay. RWPE1 cells (10,000 cells/well) were mixed with 0.4% agarose in growth medium and plated on ... shRNA control (shLuc). (D) Soft-agar colony formation capability of RWPE1 with indicated treatment: 1. μ. M. iAs; shLuc, ...

The base layer was prepared with 0.5% agar. Approximately 2,500 cells were suspended in medium with 0.35% agar and seeded over ... C) Soft agar assay of BEL-7404 cells exposed to 0.1% DMSO or different concentrations of B[a]P for 1 month. Bars = 1 mm for 4× ... Soft agar assay. For determination of anchorage-independent growth, BEL-7404 cells were plated into 6-well plates containing ... All groups formed cell clones in soft agar, and no variation in clone formation was observed (Figure 1C). These results ...

The base layer was prepared with 0.5% agar. Approximately 2,500 cells were suspended in medium with 0.35% agar and seeded over ... C) Soft agar assay of BEL-7404 cells exposed to 0.1% DMSO or different concentrations of B[a]P for 1 month. Bars = 1 mm for 4× ... Soft agar assay. For determination of anchorage-independent growth, BEL-7404 cells were plated into 6-well plates containing ... All groups formed cell clones in soft agar, and no variation in clone formation was observed (Figure 1C). These results ...

C) Colony formation in soft agar; mean number of colonies per dish was 3 (control) and 126 (29 weeks of cadmium exposure). (D) ... F) Colony formation in soft agar assay with pancreaspheres derived from CCE or control cells. Data represent mean ± SE (n = 3 ... We performed colony formation in soft agar as described (Tokar et al. 2010b). The formation of colonies of cells with the ... capacity for anchorage-independent growth in agar is accepted as a reflection of cancer phenotype. ...

C) Colony formation in soft agar; mean number of colonies per dish was 3 (control) and 126 (29 weeks of cadmium exposure). (D) ... F) Colony formation in soft agar assay with pancreaspheres derived from CCE or control cells. Data represent mean ± SE (n = 3 ... We performed colony formation in soft agar as described (Tokar et al. 2010b). The formation of colonies of cells with the ... capacity for anchorage-independent growth in agar is accepted as a reflection of cancer phenotype. ...

We used Andersen two-stage samplers to collect bioaerosol samples from 24 houses in El Paso, Texas, using tryptic soy agar as ... The Andersen two-stage samplers were loaded with plates of tryptic soy agar (TSA; Difco Laboratories, Detroit, MI), an ... Room temperature Mueller-Hinton agar (MHA; Difco Laboratories) plates were used to evaluate each of the microorganisms for ... excellent general agar used to culture a variety of bacterial microorganisms. Duplicate 10- and 15-min air samples were taken ...

We used Andersen two-stage samplers to collect bioaerosol samples from 24 houses in El Paso, Texas, using tryptic soy agar as ... The Andersen two-stage samplers were loaded with plates of tryptic soy agar (TSA; Difco Laboratories, Detroit, MI), an ... Room temperature Mueller-Hinton agar (MHA; Difco Laboratories) plates were used to evaluate each of the microorganisms for ... excellent general agar used to culture a variety of bacterial microorganisms. Duplicate 10- and 15-min air samples were taken ...

Soft agar dishes were prepared with underlayers of 0.70% agarose in DMEM supplemented with 10% FBS. To test for soft-agar ... Photomicrographs of cell colonies in soft agar; bars = 500 μm. (D) The number (mean ± SD) of cell colonies in soft agar (n = 3 ... A549 cells served as the positive control (Con). (A) Cell colonies in soft agar; bars = 500 μm. (B) Number of cell colonies in ... In contrast, decreases in NKRF, increases in p-RelA and mot-2, and increases in cell colony numbers in soft agar and tumor ...

Soft agar dishes were prepared with underlayers of 0.70% agarose in DMEM supplemented with 10% FBS. To test for soft-agar ... Photomicrographs of cell colonies in soft agar; bars = 500 μm. (D) The number (mean ± SD) of cell colonies in soft agar (n = 3 ... A549 cells served as the positive control (Con). (A) Cell colonies in soft agar; bars = 500 μm. (B) Number of cell colonies in ... In contrast, decreases in NKRF, increases in p-RelA and mot-2, and increases in cell colony numbers in soft agar and tumor ...

MacConkey agar for coliforms, fecal coliform agar for fecal coliforms, and selective Streptococcus agar for isolation group A ... 2002) placed R2A agar in Andersen samplers; and Chapin et al. (2005) used mE agar for the isolation of Enterococcus isolates ... The sampler was loaded with plates of tryptic soy agar (TSA; Difco Laboratories, Detroit, MI), an excellent general agar known ... 1966). Three Mueller-Hinton agar plates and three TSA plates were brought to room temperature and dried for each microorganism ...

EDTA; pH 7.5) and plated onto the agar plate. The transfected cells were selected on synthetic defined (SD) minimal medium (for ... details, see Table S3) solidified with agar at 30°C for 2-3 d. The yeast two-hybrid experiment was carried out as described ...

EDTA; pH 7.5) and plated onto the agar plate. The transfected cells were selected on synthetic defined (SD) minimal medium (for ... details, see Table S3) solidified with agar at 30°C for 2-3 d. The yeast two-hybrid experiment was carried out as described ...

Soft agar plates were prepared using two agar densities. The base layer contained 0.8% Noble Agar (Sigma) dissolved in H2O and ... N,O) Representative phase-contrast images (10×; N) and the number of colonies (O) of HEK293T cells grown in soft agar and ... Cells pretreated with As2O3 as described above were plated at a density of 2.5 × 103 in a 0.4% Noble Agar top layer, and ... UROtsa cells were used because they exhibit normal characteristics with no colony formation in soft agar and are ideal for ...

C) Colony formation from spheres in soft agar after co-culture. (D) Ductal-like structure formed by single cell from the ... Anchorage-independent growth. Colony formation in soft agar from cells derived from free-floating spheres is related to the NSC ... After noncontact co-culture, floating spheroid cells were separated from adherent cells, and colony formation in soft agar was ... colony formation in agar, and aggressive xenograft malignancies (Achanzar et al. 2002; Tokar et al. 2010b). ...

Each 3GCR-EC isolate was reactivated on MacConkey agar supplemented with ceftriaxone (. 2. mg. /. L. ), at 37°C for 18 h. ... Fecal samples were plated onto MacConkey agar (Difco) supplemented with ceftriaxone (. 2. mg. /. L. ), a third-generation ... The identity of presumptive E. coli colonies was confirmed by culture on Chromocult coliform agar (Merck KGaA), at 37°C for 24 ... Performance validation of chromogenic coliform agar for the enumeration of Escherichia coli and coliform bacteria. Lett Appl ...

We used mE agar for the detection and enumeration of Enterococcus spp., mTEC agar for the detection of E. coli, and mFC agar ... After 48 hr, membrane filters from mE agar plates were placed on esculin iron agar (EIA) plates and incubated at 41.5°C for 20 ... ranging from pink to dark red on mE agar and producing a brown to black precipitate on EIA agar, were considered presumptive ... Incubation conditions for the agar plates were as follows: mE plates, 41.5°C for 48 hr; mTEC plates, 35°C for 2 hr followed by ...

1979). Recently, we have shown that the expression of HIF-1α is required for the induction of soft agar growth by Ni(II) ( ... Gumuslu S, Korgun DK, Bilmen S, Yargicoglu P, Agar A. . 2000. Effects of sulfur dioxide inhalation on plasma vitamin C and ... 1982). Moreover, addition of ascorbate inhibited soft agar growth of rat osteosarcoma or human neuroblastoma cells (Girgert et ...

We used mE agar for the detection and enumeration of Enterococcus spp., mTEC agar for the detection of E. coli, and mFC agar ... After 48 hr, membrane filters from mE agar plates were placed on esculin iron agar (EIA) plates and incubated at 41.5°C for 20 ... ranging from pink to dark red on mE agar and producing a brown to black precipitate on EIA agar, were considered presumptive ... Incubation conditions for the agar plates were as follows: mE plates, 41.5°C for 48 hr; mTEC plates, 35°C for 2 hr followed by ...

C) Colony formation from spheres in soft agar after co-culture. (D) Ductal-like structure formed by single cell from the ... Anchorage-independent growth. Colony formation in soft agar from cells derived from free-floating spheres is related to the NSC ... After noncontact co-culture, floating spheroid cells were separated from adherent cells, and colony formation in soft agar was ... colony formation in agar, and aggressive xenograft malignancies (Achanzar et al. 2002; Tokar et al. 2010b). ...

We assessed effects of chronic cadmium exposure on cellular ability to form colonies when plated in soft agar as described by ... C) Increased colony formation in soft agar with chronic cadmium exposure. (D) Increased invasive ability with cadmium exposure ... Cadmium also markedly increased colony formation in soft agar by 40 weeks of exposure (Figure 1C), which is typical of cancer ...

1979). Recently, we have shown that the expression of HIF-1α is required for the induction of soft agar growth by Ni(II) ( ... Gumuslu S, Korgun DK, Bilmen S, Yargicoglu P, Agar A. . 2000. Effects of sulfur dioxide inhalation on plasma vitamin C and ... 1982). Moreover, addition of ascorbate inhibited soft agar growth of rat osteosarcoma or human neuroblastoma cells (Girgert et ...

Each 3GCR-EC isolate was reactivated on MacConkey agar supplemented with ceftriaxone (. 2. mg. /. L. ), at 37°C for 18 h. ... Fecal samples were plated onto MacConkey agar (Difco) supplemented with ceftriaxone (. 2. mg. /. L. ), a third-generation ... The identity of presumptive E. coli colonies was confirmed by culture on Chromocult coliform agar (Merck KGaA), at 37°C for 24 ... Performance validation of chromogenic coliform agar for the enumeration of Escherichia coli and coliform bacteria. Lett Appl ...

Soft agar colony growth assays were applied to examine iAs-induced transformation. For in vivo study, PrSPCs mixed with rat ...

Colony formation in soft agar was performed as described (Tokar et al. 2005) on cells from free-floating spheres. ...

... agar before mixing and pouring onto a tryptic soy agar plate. Five replicates were assayed for a total of 10 mL. Overlays were ... Total coliform and E. coli were analyzed by a filtration/agar method (American Public Health Association et al. 1995) and by ... We analyzed coliphages using the double agar layer (DAL) method or enrichment method as described in U.S. EPA Methods 1601 and ... Method 1602: Male-specific (F+) and Somatic Coliphage in Water by Single Agar Layer (SAL) Procedure. Washington DC:U.S. ...

... agar before mixing and pouring onto a tryptic soy agar plate. Five replicates were assayed for a total of 10 mL. Overlays were ... Total coliform and E. coli were analyzed by a filtration/agar method (American Public Health Association et al. 1995) and by ... We analyzed coliphages using the double agar layer (DAL) method or enrichment method as described in U.S. EPA Methods 1601 and ... Method 1602: Male-specific (F+) and Somatic Coliphage in Water by Single Agar Layer (SAL) Procedure. Washington DC:U.S. ...

Conidia were removed from these plates by pouring small aliquots of ddw+T onto the surface of the agar in each plate, gently ... Two Petri plates with conidia covering most of the agar surface were selected. ... Isolates were cultured on standard petri plates with half-strength V8 juice agar (100 mL V8 juice; Campbells Soup Company, ... Camden, NJ), 900 mL ddw, 2 g CaCO3, 15 g agar powder, autoclaved for 20 min at 121°C). ...

Conidia were removed from these plates by pouring small aliquots of ddw+T onto the surface of the agar in each plate, gently ... Two Petri plates with conidia covering most of the agar surface were selected. ... Isolates were cultured on standard petri plates with half-strength V8 juice agar (100 mL V8 juice; Campbells Soup Company, ... Camden, NJ), 900 mL ddw, 2 g CaCO3, 15 g agar powder, autoclaved for 20 min at 121°C). ...

CTX (HiMedia, Mumbai, India), was vortexed and subcultured onto MacConkey agar supplemented with 1. mg/L. CTX [Diederen et al. ... Three well-isolated E. coli-like colonies (pink, doughnut-shaped) were selected at random from the MacConkey Lactose agar plate ... A single, well-isolated colony from each trypticase soy agar plate was then suspended in 5 mL of trypticase soy broth and ... One swab from each bird was cultured on selective MacConkey Lactose agar (HiMedia, Mumbai, India), while the other was ...