*  Induction of Bach1 and ARA70 gene expression at an early stage of adipocyte differentiation of mouse 3T3-L1 cells | Biochemical...
Induction of Bach1 and ARA70 gene expression at an early stage of adipocyte differentiation of mouse 3T3-L1 cells. Makoto ... Induction of Bach1 and ARA70 gene expression at an early stage of adipocyte differentiation of mouse 3T3-L1 cells ... Induction of Bach1 and ARA70 gene expression at an early stage of adipocyte differentiation of mouse 3T3-L1 cells ... Induction of Bach1 and ARA70 gene expression at an early stage of adipocyte differentiation of mouse 3T3-L1 cells ...
  http://www.biochemj.org/content/361/3/629
*  The Carb-Sane Asylum: Adipocyte Size & Adipokine Secretion
Relationship between Adipocyte Size and Adipokine Expression and Secretion. Context: Adipocytes are known to release a variety ... The results obtained by the technique clearly suggest that only the very large adipocytes are dysregulated. Adipocyte ... Something else that caught my eye: Recently, adipocyte size in the sc abdominal depot was identified to be a significant ... Design, Patients, and Main Outcome Measures: Protein secretion and mRNA expression in cultured adipocytes separated according ...
  http://carbsanity.blogspot.com/2010/10/adipocyte-size-adipokine-secretion.html
*  Human Preadipocytes-subcutaneous | Creative Bioarray
Adipocytes play an important role in energy storage and metabolism. Adipocyte differentiation is a developmental process that ... It has also been demonstrated that there is a close relationship between adipocyte differentiation and many physiological and ... Preadipocytes are present throughout adult life in adipose tissues and can proliferate and differentiate into mature adipocytes ...
  https://www.creative-bioarray.com/Human-Preadipocytes-subcutaneous-CSC-7736W-item-1791.htm
*  Plus it
In obesity, the differentiation of adipocytes is suppressed. Although adipocyte differentiation is associated with changes in ... The M2 isoform of PK (PKM2) is expressed in adipocytes but its role in adipogenesis is unknown. Here we demonstrate that PKM2 ... Abstract B36: Pyruvate kinase M2 regulates adipocyte differentiation and the expression of enzymes involved in glucose ... Overexpression of PKM2 suppressed adipocyte differentiation. In obesity, increased numbers of macrophages are found in adipose ...
  http://cancerpreventionresearch.aacrjournals.org/content/8/10_Supplement/B36
*  Ser-474 is the major target of insulin-mediated phosphorylation of protein kinase B beta in primary rat adipocytes.
... Göransson ... Taken together, these results show that rat adipocyte PKB beta mainly is phosphorylated on Ser-474 in response to insulin ... In this study, we have characterized the insulin-induced phosphorylation and activation of PKB beta in primary rat adipocytes. ... Adipocytes/drug effects/*enzymology, Animals, Cells; Cultured, Electrophoresis; Gel; Two-Dimensional, Enzyme Inhibitors/ ...
  http://uu.diva-portal.org/smash/record.jsf?pid=diva2:38200
*  Deficiency of Phosphoinositide 3-Kinase Enhancer Protects Mice From Diet-Induced Obesity and Insulin Resistance | Diabetes
First, the mature adipocyte marker aP2 is significantly decreased during in vitro adipocyte differentiation in PIKE−/− MEFs, ... PIKE is essential for adipocyte differentiation.. Under chow diet feeding conditions, expressions of mature adipocyte markers ... Adipocyte dysfunctions linking obesity to insulin resistance and type 2 diabetes. Nat Rev Mol Cell Biol 2008; 9: 367- 377. ... 2C). The adipocytes in PIKE−/− mice were also smaller in both feeding conditions (Fig. 2D and E). Moreover, circulating leptin ...
  http://diabetes.diabetesjournals.org/content/59/4/883?with-ds=yes
*  In vitro models of diabetes - FRAME
The FRAME Alternatives Laboratory cultures primary human adipocytes and skeletal muscle myotubes to study the effects of ...
  http://www.frame.org.uk/research-diabetes/
*  Protein kinase C isoforms ɛ, η, δ and ζ in murine adipocytes: expression, subcellular localization and tissue-specific...
3T3-L1 adipocytes express PKC-ε and -ζ, and PKC-ε expression increases with differentiation from preadipocytes to adipocytes. ... PMA treatment of adipocytes induces translocation of PKC-ε and -δ from the cytosol to the membrane fractions. Insulin treatment ... The PKC isoforms ε, η, δ and ζ are expressed in adipocytes. Immunoreactivity for all isoforms is higher in the membranes than ... Protein kinase C isoforms ɛ, η, δ and ζ in murine adipocytes: expression, subcellular localization and tissue-specific ...
  http://www.biochemj.org/content/316/3/865
*  Plus it
Research Design and Methods: ApoE gene and protein expression in 3T3-L1 adipocytes, or mature adipocytes and adipose tissue ... Results: Oxidant stress in 3T3-L1 cells, or adipocytes and adipose tissue from lean mice significantly reduced apoE mRNA and ... Objective: Endogenous expression of apoE has a significant impact on adipocyte lipid metabolism and is markedly suppressed in ... Adipose tissue oxidant stress is emerging as an important mediator of adipocyte dysfunction. These studies were undertaken to ...
  http://diabetes.diabetesjournals.org/content/early/2008/08/04/db08-0592
*  Plus it
Some have proposed that the generation of new adipocytes may be balanced by adipocyte death, with the constant and tightly ... Obesity in adults is typically associated with adipocyte hypertrophy, and an additional adipocyte hyperplasia occurs in ... in 3T3-L1 cells promotes adipocyte differentiation. IFN-γ not only rescued adipocyte differentiation but also decreased Coup- ... A: 3T3-L1 cells were induced to differentiate into adipocytes in the presence of 20 nmol/L SAG and/or 50 ng/mL IFN-γ or were ...
  http://diabetes.diabetesjournals.org/content/60/6/1668
*  Consequence of Menin Deficiency in Mouse Adipocytes Derived by In Vitro Differentiation
The top 40 genes differentially expressed in the Men1-KO adipocytes (at least 3-fold change compared with WT adipocytes; ) were ... b) Expression of adipocyte marker genes. Conventional RT-PCR followed by agarose gel electrophoresis of the adipocyte marker ... These menin-deficient adipocytes and their corresponding normal adipocytes were analyzed for cell size, lipid content, and gene ... using RNA isolated from mESC-derived adipocytes showed 1.6- to 29-fold upregulation of 19 genes in menin-null adipocytes; the ...
  https://www.hindawi.com/journals/ije/2015/149826/
*  Ursolic Acid Inhibits Adipogenesis in 3T3-L1 Adipocytes through LKB1/AMPK Pathway
Adipocyte differentiation Is the Subject Area "Adipocyte differentiation" applicable to this article? Yes. No. ... Adipocytes Is the Subject Area "Adipocytes" applicable to this article? Yes. No. ... Adipose tissue is determined by the number and size of adipocytes. The increase in the number of adipocytes involves the ... Sirt1 inhibits adipogenesis by repressing PPARγ expression in cultured adipocytes [26] and decreases adipocyte formation during ...
  http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0070135
*  Homocysteine Upregulates Resistin Production From Adipocytes In Vivo and In Vitro | Diabetes
Primary cultured rat adipocytes express all PKC isoforms except PKC-γ (27). Our results show that in adipocytes, PKCs, most ... Isolation and culture of primary rat adipocytes.. Mature adipocytes were isolated from epididymal fat pads of Sprague-Dawley ... After another 24 h incubation, adipocytes treated with the conditioned medium from Hcy-treated adipocytes showed significant ... adipocytes treated with the conditioned medium from Hcy-treated adipocytes also showed a significant reduction in insulin- ...
  http://diabetes.diabetesjournals.org/content/57/4/817
*  Homocysteine Upregulates Resistin Production From Adipocytes In Vivo and In Vitro | Diabetes
Adipocyte Liver Kinase b1 Suppresses Beige Adipocyte Renaissance Through Class IIa Histone Deacetylase 4 ... Homocysteine Upregulates Resistin Production From Adipocytes In Vivo and In Vitro. Yin Li, Changtao Jiang, Guoheng Xu, Nanping ... Homocysteine Upregulates Resistin Production From Adipocytes In Vivo and In Vitro. Yin Li, Changtao Jiang, Guoheng Xu, Nanping ... 19.27 ± 1.71 ng/ml, P , 0.01). Hcy (300-1000 μmol/l) also increased mRNA expression of resistin in primary rat adipocytes in a ...
  http://diabetes.diabetesjournals.org/content/57/4/817.short?cited-by=yes&legid=diabetes
*  Patent US6492130 - Modulation of the sulfonylurea receptor and calcium in adipocytes for ... - Google Patents
... an adipocyte potassium channel activator, an SUR 1 antagonist, and an adipocyte specific SUR 1 antagonist. The present ... invention recognizes the presence of the sulfonylurea receptor in adipocytes and its utility in identifying compounds and in ... The cultured adipocytes may be derived from any animal. Preferably the adipocytes are mammalian. More preferably the adipocytes ... Ca2+]i is measured in primary adipocytes. For primary adipocytes, 3 ml of cells are resuspended in 6 ml of HBSS and fura-2/AM ...
  http://www.google.com/patents/US6492130?dq=6,123,819
*  Adipocyte Differentiation Toolkit for Adipose-Derived MSCs and Preadip
... with high efficiency and to support maturation of derived adipocytes during lipid accumulation. ... The Adipocyte Differentiation Toolkit for Adipose-derived MSCs and Preadipocytes (ATCC PCS-500-050) contains medium and ... One bottle containing 100 mL of Adipocyte Basal Medium for use in both initiation and maintenance of adipocyte differentiation ... Adipocyte Differentiation Toolkit for Adipose-Derived MSCs and Preadipocytes ATCC® PCS-500-050™ frozen 1 kit ...
  https://www.atcc.org/en/Global/Products/2/D/B/F/PCS-500-050.aspx
*  Apolipoprotein E promotes lipid accumulation and differentiation in human adipocytes (Journal Article) | SciTech Connect
Interestingly, incubation of APOE-deficient hMSC-Tert adipocytes with conditioned media from APOE3-overexpressing adipocytes or ... but were unable to induce adipocyte differentiation, asmore » judged by expression of adipocyte markers. Taken together, ... However, little is yet known if APOE functions in a similar manner in human adipocytes. This prompted us to compare lipid ... Differentiated hMSC-Tert were stably transduced with or without siRNA targeting APOE while murine adipocytes were isolated from ...
  https://www.osti.gov/scitech/biblio/22462329-apolipoprotein-promotes-lipid-accumulation-differentiation-human-adipocytes
*  IJMS | Free Full-Text | Lipopolysaccharide Modifies Glycerol Permeability and Metabolism in 3T3-L1 Adipocytes
In adipocytes, aquaglyceroporins mediate glycerol uptake and release across the plasma membrane, which are two key steps for ... Nevertheless, our findings provide novel insights in understanding the LPS-induced adipocyte hypertrophy that accompanies ... treated differentiated 3T3-L1 adipocytes. Glycerol release, TAGs content and whole membrane glycerol permeability were ... In adipocytes, aquaglyceroporins mediate glycerol uptake and release across the plasma membrane, which are two key steps for ...
  http://www.mdpi.com/1422-0067/18/12/2566
*  Alliin, a Garlic (Allium sativum) Compound, Prevents LPS-Induced Inflammation in 3T3-L1 Adipocytes
2 phosphorylation in LPS-stimulated 3T3-L1 adipocytes and generating an anti-inflammatory gene expression profile in adipocytes ... "Salicylate inhibits macrophage-secreted factors induced adipocyte inflammation and changes of adipokines in 3T3-L1 adipocytes ... To obtain total protein from 3T3-L1 adipocytes, cells from different treatments were homogenized in 50 μL RIPA buffer at 4°C ... Alliin, a Garlic (Allium sativum) Compound, Prevents LPS-Induced Inflammation in 3T3-L1 Adipocytes. Saray Quintero-Fabián,1,2 ...
  https://www.hindawi.com/journals/mi/2013/381815/
*  Frontiers | AdipoCount: A New Software for Automatic Adipocyte Counting | Physiology
... of adipocytes. In clinical and scientific research, the accurate quantification of the number and diameter of adipocytes is ... of adipocytes. In clinical and scientific research, the accurate quantification of the number and diameter of adipocytes is ... One typical feature of obesity is the excessive accumulation of fat in adipocytes, which occurs through the following two ... In this study, we present a new automatic adipocyte counting system, AdipoCount, which is based on image processing algorithms ...
  https://www.frontiersin.org/articles/10.3389/fphys.2018.00085/full
*  Plus it
In females, PG adipocytes were 20% bigger than SC adipocytes, whereas in males, PG adipocytes were 60% bigger than SC ... and male adipocytes from either depot (0.4-0.7 nmol/l). Female PG adipocytes have a higher lipogenic rate than male adipocytes ... when compared with adipocytes from the sham controls, more closely resembling those of male adipocytes. PG adipocytes from ... and ACC in female adipocytes from both depots when compared with adipocytes from sham-operated mice, whereas in male adipocytes ...
  http://diabetes.diabetesjournals.org/content/58/4/803
*  Adipocytes Cause Leukemia Cell Resistance to L-Asparaginase via Release of Glutamine | Cancer Research
As adipocytes produce both ASN and GLN, we next tested whether either of these amino acids were responsible for adipocyte ... Adipocytes protect leukemia cells from ASNase via GLN production. To determine whether adipocytes could protect ALL cells from ... C, 8093 cells plated over no feeder (N), 3T3-L1 adipocytes (A), or MSO-treated adipocytes (A-MSO) in the presence of 1 IU/mL ... 3A). A similar pattern was observed with adipocytes differentiated from OP9 bone marrow mesenchymal cells (Fig. 3B). Adipocyte ...
  http://cancerres.aacrjournals.org/content/73/10/2998