Frontiers | An altered transcriptome underlies cln5-deficiency phenotypes in Dictyostelium discoideum
FIGURE 2. FIGURE 2. Loss of cln5 affects the intracellular and extracellular levels of AprA and CfaD during growth. Whole cell ... 2 Materials and methods. 2.1 Cell lines, media, and antibodies. AX3 (hereafter referred to as WT, parental cell line of cln5-) ... The samples were left at 37°C for 1 h and then an equal volume of 0.5 M Na2CO3/NaHCO3 buffer containing 0.025% (v/v) Triton X- ... 2.4.2 α-glucosidase. Methods were adapted from Wimmer et al. (1997). Briefly, WC lysates were added to 0.1 M sodium succinate ( ...
Frontiers | Fed-Batch Cultivations of Rhodospirillum rubrum Under Multiple Nutrient-Limited Growth Conditions on Syngas as a...
FIGURE 2. Figure 2. Growth of R. rubrum in serum vials with different syngas concentrations. R. rubrum cultures were grown on ... Stephanie Karmann1,2 Sven Panke2 Manfred Zinn1*. *1Institute of Life Technologies, University of Applied Sciences and Arts ... R2 = 0.99), the biomass correlated with the cell number with a factor of 1.5 1012 cells g−1 biomass (R2 = 0.93). ... Carbon (as CO2) and hydrogen (as H2O) were quantified by infrared spectroscopy. The nitrogen content (as N2) was determined by ...
Frontiers | Single-Nucleotide RNA Maps for the Two Major Nosocomial Pathogens Enterococcus faecalis and Enterococcus faecium
... encodes propanol dehydrogenase PduQ. On the right, fading in the treated sample indicates that the bar continues and is cut to ... Supplementary Figure 2 , E. faecalis and E. faecium promoter motifs for detected TSS. (A) The −10 box motif for the σ70 ... FIGURE 2. Figure 2 Transcription factor binding site motifs. (A), The −10 box motif for the σ70 transcription factor detected ... Laura Jenniches2 Milan Gerovac1 Lars Barquist2,3* Jörg Vogel1,2* ... 2Helmholtz Institute for RNA-based Infection Research (HIRI), ...
Frontiers | Glucose Oxidase/Nano-ZnO/Thin Film Deposit FTO as an Innovative Clinical Transducer: A Sensitive Glucose Biosensor
Diffusional electrochemistry of cytochrome c on mixed captopril/3-mercapto-1-propanol self-assembled monolayer modified gold ... FIGURE 2. Figure 2. Effect of (A) DC magnetron sputtering power, (B) deposition time, (C) target-to-substrate distance, (D) ... K2HPO4 and KH2PO4 were purchased from Sigma-Aldrich Co. and used for the preparation of phosphate-buffered saline (PBS; 7.4). A ... TABLE 2. Table 2. Analytical characteristics GOx/Nano-ZnO/PVA/FTO biosensor compared with the other reported ZnO base glucose ...