Artemisia: A plant genus of the family ASTERACEAE with strong-smelling foliage. It is a source of SANTONIN and other cytotoxic TERPENES.Artemisia annua: A plant species of the genus ARTEMISIA, family ASTERACEAE. It is the source of the antimalarial artemisinin (ANTIMALARIALS).Artemisia absinthium: A plant species of the genus ARTEMISIA, family ASTERACEAE that has been used in ABSINTHE. The oil contains neurotoxic 1-thujone and d-isothujone.Oils, Volatile: Oils which evaporate readily. The volatile oils occur in aromatic plants, to which they give odor and other characteristics. Most volatile oils consist of a mixture of two or more TERPENES or of a mixture of an eleoptene (the more volatile constituent of a volatile oil) with a stearopten (the more solid constituent). The synonym essential oils refers to the essence of a plant, as its perfume or scent, and not to its indispensability.Plant Extracts: Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away, and adjusting the residue to a prescribed standard.Asteraceae: A large plant family of the order Asterales, subclass Asteridae, class Magnoliopsida. The family is also known as Compositae. Flower petals are joined near the base and stamens alternate with the corolla lobes. The common name of "daisy" refers to several genera of this family including Aster; CHRYSANTHEMUM; RUDBECKIA; TANACETUM.Artemisinins: A group of SESQUITERPENES and their analogs that contain a peroxide group (PEROXIDES) within an oxepin ring (OXEPINS).Plant Components, Aerial: The above-ground plant without the roots.SesquiterpenesPlants, Medicinal: Plants whose roots, leaves, seeds, bark, or other constituent parts possess therapeutic, tonic, purgative, curative or other pharmacologic attributes, when administered to man or animals.

*  1 oz Tarragon (Artemisia dracunculus) Organic & Kosher France
Also known asArtemisia dracunculus, French Tarragon, German Tarragon, Dragon Wormwood, Dragonwort, Dragon Mugwort and sometimes called both Mugwort an
  https://oursacredgarden.ecrater.com/p/26003090/1-oz-tarragon-artemisia-dracunculus-organic
*  Artemisia afra - Wikimedia Commons
Artemisia • Species: Artemisia afra Jacq. ex Willd., 1804 ... Artemisia afra. From Wikimedia Commons, the free media ... Retrieved from "https://commons.wikimedia.org/w/index.php?title=Artemisia_afra&oldid=142243367" ...
  https://commons.wikimedia.org/wiki/Artemisia_afra
*  ARTEMISIA VULGARIS. - THE ENCYCLOPEDIA OF PURE MATERIA MEDICA By TIMOTHY F. ALLEN, A.M., M.D.
ARTEMISIA VULGARIS.. A. vulgaris, Linn. Nat. order, Composit . Common names, Wormwood, Mugwort, Beifuss, etc. Preparation, ...
  http://www.homeoint.org/allen/a/art-v.htm
*  Chemical characterization of a novel polysaccharide ASKP-1 from Artemisia sphaerocephala Krasch seed and its macrophage...
... aim of this study was to investigate the molecular mechanism underlying the immunomodulatory effect of the purified Artemisia ... Chemical characterization of a novel polysaccharide ASKP-1 from Artemisia sphaerocephala Krasch seed and its macrophage ... Chemical characterization of a novel polysaccharide ASKP-1 from Artemisia sphaerocephala Krasch seed and its macrophage ... aim of this study was to investigate the molecular mechanism underlying the immunomodulatory effect of the purified Artemisia ...
  http://pubs.rsc.org/en/content/articlelanding/2017/fo/c6fo01699e
*  Genome size dynamics in Artemisia L. (Asteraceae): following the track of polyploidy - Pellicer - 2009 - Plant Biology - Wiley...
Artemisia, Asteraceae) in Turkey, Caryologia, 2014, 67, 4, 305. CrossRef. *9. Sònia Garcia, Ilia J. Leitch, Alba Anadon-Rosell ... Table S1. Provenance of the Artemisia populations studied.. Please note: Wiley-Blackwell are not responsible for the content or ... Genome size dynamics in Artemisia L. (Asteraceae): following the track of polyploidy. Authors. *. J. Pellicer,. * Laboratori de ... We confirmed that the increase in 2C values in Artemisia polyploids was not proportional to ploidy level, but 1Cx genome size ...
  http://onlinelibrary.wiley.com/doi/10.1111/j.1438-8677.2009.00268.x/abstract
*  Some pharmacological studies on Artemisia herba-alba (Asso.) in rabbits and mice. - Semantic Scholar
The aqueous extract of Artemisia herba-alba Asso. (Compositae) produced an initial hyperglycaemia which was followed by ... Some pharmacological studies on Artemisia herba-alba (Asso.) in rabbits and mice.. *. Husnia I. Marrif. , Badreldin H Ali. , ... The Potential Anti Helicobacter pylori and antioxidant effects of Artemisia Judaica. *M. Adel El-Sayed. , Radwan BaAbbad. , ... Relaxant Effect of Essential Oil of Artemisia herba-alba Asso. on Rodent Jejunum Contractions. *Mohammed Aziz. , Ahmed Karim. ...
  https://www.semanticscholar.org/paper/Some-pharmacological-studies-on-Artemisia-herba-al-Marrif-Ali/f9b4bd1aa3ce2e19f6033dddb02056be9fe898f3
*  If Insulin Sensitivity is Key, What's the Key to Insulin Sensi- tivity? Artemisia Dracunculus, Lixisenatide, Calcium & Bi-...
What do Artemisia dracunculus, Lixisenatide, calcium and baking soda have in common? They all may have a place in "anti- ... Artemisia dracunculus and Lixisenatide, two names to remember?. Actually the first of these names, namely Artemesia dracunculus ... "An extract of Artemisia dracunculus L. inhibits ubiquitin-proteasome activity and preserves skeletal muscle mass in a murine ... "An extract of Artemisia dracunculus L. enhances insulin receptor signaling and modulates gene expression in skeletal muscle in ...
  http://suppversity.blogspot.com/2014/03/if-insulin-sensitivity-is-key-whats-key.html
*  Vascular Plants of the Gila Wilderness-- Artemisia dracunculus
Artemisia dracunculoides Pursh. Artemisia dracunculoides Pursh var. dracunculina (S. Watson) Blake. Artemisia glauca Pallas ex ... Artemisia dracunculus Linnaeus. (Tarragon). Family: Asteraceae. Status: Native Synonyms:. Artemisia dracunculina S. Watson. ... Artemisia dracunculus, photo Russ Kleinman & Kelly Kindscher, Lake Roberts, July 15, 2007. Artemisia dracunculus, photo Russ ... Artemisia dracunculus can be recognized by its dark green leaves and reddish stems. The flower heads are nodding. Please click ...
  http://wnmu.edu/academic/nspages/gilaflora/artemisia_dracunculus.html
*  Artemisia bei Psoriasis
Willcox M, Artemisia bei Psoriasis G, Bourdy G, Artemisia bei Psoriasis al. Artemisia annua as a Traditional Herbal ... Green tea Artemisia bei Psoriasis particularly Artemisia bei Psoriasis in the catechin class of Artemisia bei Psoriasis ... It has been removed from most of our foods Artemisia bei Psoriasis of big Artemisia bei Psoriasis. Our soils are depleted of ... Yang S, Artemisia bei Psoriasis J, Guo Z, et al.. Triptolide inhibits the growth Artemisia bei Psoriasis metastasis of solid ...
  http://outdoor-frauen.de/artemisia-bei-psoriasis.php
*  Mugwort - Wikipedia
Artemisia stelleriana - hoary mugwort Artemisia verlotiorum - Chinese mugwort Artemisia vulgaris - common mugwort, used as a ... alpine mugwort Artemisia indica - Oriental mugwort Artemisia japonica - Japanese mugwort Artemisia norvegica - Norwegian ... Artemisia argyi - Chinese mugwort, used in traditional Chinese medicine Artemisia douglasiana - Douglas mugwort or California ... Mugwort is a common name for several species of aromatic plants in the genus Artemisia. In Europe, mugwort most often refers to ...
  https://en.wikipedia.org/wiki/Mugwort
*  Artemisia - Wikipedia
Artemisia can mean: Artemisia I of Caria (fl. 480 BC), queen of Halicarnassus under the First Persian Empire, naval commander ... US Artemisia, Messinia, Greek village west of Taygetus mountain in the Peloponnese Artemisia pipe, diatreme in the Northwest ... opera by Cavalli Artemisia (Dresden, 1754), opera seria by Johann Adolph Hasse Artemisia (Goldoni), unperformed 18th century ... Like Dust by Isaac Asimov Artemisia, main character of Moira by Sound Horizon Artemisia (plant), genus of plants including the ...
  https://en.wikipedia.org/wiki/Artemisia
*  Artemisia pontica - Wikipedia
Artemisia. CRC Press. p. 59. Retrieved 2014-08-20. Botanical Museum, Helsinki, Finland, distribution map for Artemisia pontica ... Artemisia pontica is called "little absinthe" because it is smaller in stature and leaf than the "great absinthe" A. absinthium ... Artemisia pontica, the Roman wormwood or small absinthe, is an herb used in the production of absinthe and vermouth. ... 20-21 Page 687 西北蒿 xi bei hao Artemisia pontica Linnaeus, Sp. Pl. 2: 847. 1753. Biota of North America Program 2014 county ...
  https://en.wikipedia.org/wiki/Artemisia_pontica
*  Artemisia cina - Wikipedia
Artemisia cina, commonly known as santonica (zahr el shieh el -khorasani), Levant wormseed, and wormseed, is an Asian species ... ISBN 978-0-486-22799-3. "Artemisia cina". Germplasm Resources Information Network (GRIN). Agricultural Research Service (ARS), ...
  https://en.wikipedia.org/wiki/Artemisia_cina
*  Artemisia frigida - Wikipedia
"RHS Plant Selector - Artemisia frigida". Retrieved 21 July 2013. Artemisia frigida. USDA NRCS Plant Guide. Retrieved December ... 20-21 Page 679 冷蒿 leng hao Artemisia frigida Willdenow, Sp. Pl. 3: 1838. 1803. McWilliams, Jack (2003). Artemisia frigida. In: ... Artemisia frigida is a perennial plant but with a woody base. The stems spread out, generally forming a mat or clump up to 40 ... Artemisia frigida is a widespread species of flowering plant in the aster family, which is known as the sunflower family. It is ...
  https://en.wikipedia.org/wiki/Artemisia_frigida
*  Artemisia Geyser - Wikipedia
Artemisia Geyser is a geyser in the Upper Geyser Basin of Yellowstone National Park in the United States. Artemisia Geyser is ... "Artemisia Geyser Statistics for 2009". Geysers of Yellowstone. Geyser Observation and Study Association (GOSA). "Artemisia ... Artemisia also experiences minor eruptions lasting about 5 minutes. These minor eruptions are followed by major eruptions ... The fountain reaches a height of 30 feet (9.1 m). Artemisia's pool overflows quietly for many hours before an eruption, but ...
  https://en.wikipedia.org/wiki/Artemisia_Geyser
*  Artemisia rigida - Wikipedia
The Plant List Artemisia rigida (Nutt.) A.Gray Artemisia rigida. Flora of North America. Biota of North America Program 2014 ... Artemisia rigida. USDA FS International Institute of Tropical Forestry. Daubenmire, R. (1982). The distribution of Artemisia ... Artemisia rigida provides food for wildlife because it is often the only plant exposed as the snow melts in the spring on ... Artemisia rigida is a species of flowering plant in the aster family known by the common names scabland sagebrush and stiff ...
  https://en.wikipedia.org/wiki/Artemisia_rigida
*  Artemisia porteri - Wikipedia
Artemisia porteri. Archived October 26, 2011, at the Wayback Machine. Center for Plant Conservation. Artemisia porteri. The ... Artemisia porteri is a species of flowering plant in the aster family known by the common names Porter's sagebrush, Porter's ...
  https://en.wikipedia.org/wiki/Artemisia_porteri
*  Artemisia pygmaea - Wikipedia
The Plant List Artemisia pygmaea A.Gray Flora of North America, Pygmy sage, Artemisia pygmaea A. Gray Biota of North America ... The Nature Conservancy: Artemisia pygmaea Artemisia pygmaea in the CalPhotos Photo Database, University of California, Berkeley ... Artemisia pygmaea is a small, cushion-like shrub growing up to about 20 centimeters (8 inches) in height. It is woody and grows ... Artemisia pygmaea is a North American species of sagebrush in the aster family known by the common name pygmy sagebrush. ...
  https://en.wikipedia.org/wiki/Artemisia_pygmaea
*  Artemisia filifolia - Wikipedia
The Plant List Artemisia filifolia Torr. Flora of North America Vol. 19, 20 and 21 Page 508 Sand sage Artemisia filifolia ... Artemisia filifolia is a branching woody shrub growing up to 1.5 metres (4 ft 11 in) tall. The stems are covered narrow, ... Artemisia filifolia, known by common names including sand sagebrush, sand sage and sandhill sage, is a species of flowering ... Artemisia filifolia is a dominant species across much of the west-central United States, particularly in areas where the ...
  https://en.wikipedia.org/wiki/Artemisia_filifolia
*  Artemisia biennis - Wikipedia
Artemisia biennis is a species of sagebrush known by the common name biennial wormwood. It is a common and widely distributed ... "Artemisia biennis". Natural Resources Conservation Service PLANTS Database. USDA. Retrieved 6 July 2016. Flora of North America ... 19, 20 and 21 Page 523 Biennial wormwood, armoise bisannuelle Artemisia biennis Willdenow, Phytographia. 11. 1794. Biota of ...
  https://en.wikipedia.org/wiki/Artemisia_biennis
*  Artemisia pedatifida - Wikipedia
The Plant List Artemisia pedatifida Nutt. Taylor, Jane E. 2006. Artemisia pedatifida. In: Fire Effects Information System, [ ... Artemisia pedatifida is a species of flowering plant in the aster family known by the common names birdfoot sagebrush and ... Artemisia pedatifida. Flora of North America. The Nature Conservancy. ...
  https://en.wikipedia.org/wiki/Artemisia_pedatifida
*  Artemisia furcata - Wikipedia
The Plant List Artemisia furcata M.Bieb. "Artemisia furcata". Natural Resources Conservation Service PLANTS Database. USDA. ... Artemisia furcata, the forked wormwood, is an Asian and North American species of plants in the sunflower family found in cold ... Artemisia furcata is a perennial up to 35 cm (14 inches) tall, not generally forming clumps. Leaves are gray-green, some ... Biota of North America Program 2014 state-level distribution map Pan-arctic Flora, 862207 Artemisia furcata M. Bieb. Flora of ...
  https://en.wikipedia.org/wiki/Artemisia_furcata
*  Artemisia umbelliformis - Wikipedia
Artemisia gabriellae Br.-Bl. (1945) Artemisia genipi subsp. eriantha Artemisia laxa Fritsch (1893) Artemisia laxiflora St-Lager ... Artemisia campestris Artemisia × seileri FO Wolf (1892) - Hybrid with: Artemisia glacialis Artemisia × sylviana FO Wolf (1892 ... Artemisia glacialis L., Artemisia nitida Bertol., and Artemisia lanata Willd .. Other interspecific hybrids: Artemisia × ... 1889) Artemisia mutellina Vill. (1779) Artemisia oligantha Miégeville (1872) Artemisia petrosa (Baumg.) Jan Artemisia villarsii ...
  https://en.wikipedia.org/wiki/Artemisia_umbelliformis
*  Artemisia (genus) - Wikipedia
Artemisia tridentata Artemisia cana Artemisia nova Artemisia rigida Artemisia arbuscula Artemisia longiloba Artemisia ... Artemisia persica Boiss. Artemisia pewzowii C.G.A.Winkl. Artemisia phaeolepis Krasch. Artemisia polysticha Poljakov Artemisia ... Artemisia deserti Krasch. Artemisia desertorum Spreng. Artemisia diffusa Krasch. ex Poljakov Artemisia dimoana Popov Artemisia ... Artemisia incana (L.) Druce Artemisia indica Willd. - yomogi Artemisia insulana Krasch. Artemisia insularis Kitam. Artemisia ...
  https://en.wikipedia.org/wiki/Artemisia_(genus)
*  Artemisia Gentileschi - Wikipedia
Artemisia befriended Tuzia; however, Tuzia allowed Agostino Tassi and Cosimo Quorlis to accompany Artemisia in Artemisia's home ... Artemisia felt betrayed by Tuzia, and because Tuzia was the only female figure in her life, Artemisia's works contained a ... The film Artemisia (1997), by Agnès Merlet, tells the story of Artemisia's entry into being a professional artist, her ... Artemisia Gentileschi was born Artemisia Gentileschi-Lomi in Rome on 8 July 1593, although her birth certificate from the ...
  https://en.wikipedia.org/wiki/Artemisia_Gentileschi

Artemisia afra: Artemisia afra is a common species of the genus Artemisia in Africa, with a wide distribution from South Africa, to areas reaching to the North and East, as far north as Ethiopia. Artemisia afra is the only indigenous species in this genus.Artemisia annua: Artemisia annua, also known as sweet wormwood, sweet annie, sweet sagewort, annual mugwort or annual wormwood (), is a common type of wormwood native to temperate Asia, but naturalized in many countries including scattered parts of North America.Flora of China Vol.Essential oil: An essential oil is a concentrated hydrophobic liquid containing volatile aroma compounds from plants. Essential oils are also known as volatile oils, ethereal oils, aetherolea, or simply as the oil of the plant from which they were extracted, such as oil of clove.PhytomedicineTithonia diversifoliaPfATP6: PfATP6, also known as PfSERCA or PfATPase6, is a calcium ATPase gene encoded by the malaria parasite Plasmodium falciparum. The protein is thought to be a P-type ATPase involved in calcium ion transport.Shatter (novel): Shatter is a psychological thriller written by the Australian author Michael Robotham that was published in 2008. Professor Joseph O'Loughlin (referred to as Joe throughout the novel) is tasked by the police with stopping a woman, Christine Wheeler, from committing suicide, only to fail.Sesquiterpene lactone: Sesquiterpene lactones are a class of chemical compounds; they are sesquiterpenoids (built from three isoprene units) and contain a lactone ring, hence the name. They are found in many plants and can cause allergic reactions and toxicity if overdosed, particularly in grazing livestock.Medicinal plants of the American West: Many plants that grow in the American West have use in traditional and herbal medicine.

(1/155) The cyanogenic glucoside, prunasin (D-mandelonitrile-beta-D-glucoside), is a novel inhibitor of DNA polymerase beta.

A DNA polymerase beta (pol. beta) inhibitor has been isolated independently from two organisms; a red perilla, Perilla frutescens, and a mugwort, Artemisia vulgaris. These molecules were determined by spectroscopic analyses to be the cyanogenic glucoside, D-mandelonitrile-beta-D-glucoside, prunasin. The compound inhibited the activity of rat pol. beta at 150 microM, but did not influence the activities of calf DNA polymerase alpha and plant DNA polymerases, human immunodefficiency virus type 1 reverse transcriptase, calf terminal deoxynucleotidyl transferase, or any prokaryotic DNA polymerases, or DNA and RNA metabolic enzymes examined. The compound dose-dependently inhibited pol. beta activity, the IC(50) value being 98 microM with poly dA/oligo dT(12-18) and dTTP as the DNA template and substrate, respectively. Inhibition of pol. beta by the compound was competitive with the substrate, dTTP. The inhibition was enhanced in the presence of fatty acid, and the IC(50) value decreased to approximately 40 microM. In the presence of C(10)-decanoic acid, the K(i) value for substrate dTTP decreased by 28-fold, suggesting that the fatty acid allowed easier access of the compound to the substrate-binding site.  (+info)

(2/155) Inhibitory effect of Artemisia asiatica alkaloids on acetylcholinesterase activity from rat PC12 cells.

We screened 42 Korean traditional tea plants to determine the inhibitory effect of acetylcholinesterase and attenuation of toxicity induced by amyloid-beta peptide, which were related to the treatment of Alzheimer's disease (AD). The methanolic extract from Artemisia asiatica among tested 42 tea plants, showed the highest inhibitory effect (48%) on acetylcholinesterase in vitro. The methanolic extract was further separated with n-hexane, chloroform, and ethyl acetate of water, in order. The chloroform solubles, which were high in inhibitory effect of acetylcholinesterase, were repeatedly subjected to open column chromatography on silica gel. From the highest inhibitory fraction (78%) on acetylcholinesterase, the single compound was obtained by the Sep-Pak Cartridge (C18: reverse phase column). This compound was found to react positively on Dragendorff's reagent (potassium bismuth iodide), which typically reacted with the alkaloid. This compound was purified by HPLC (mu-bondapack C18 reverse phase column: 3.9 x 150 mm). The IC50 (the concentration of 50% enzyme inhibition) value of this compound was 23 micrograms/ml and the inhibitory pattern on acetylcholinesterase was mixed with competitive/non-competitive type. We examined the effects of this compound on toxicity induced by A beta (25-35) in rat pheochromocytoma PC12 cells. Pretreatment of the PC12 cells for 2 h with an alkaloid of Artemisia asiatica (1200 microg/ml) reduced the toxicity induced by A beta. This study demonstrated that an alkaloid of Artemisia asiatica, which was metabolized to small molecule in digestive tract and then could pass through the blood-brain barrier, appeared to be an acetylcholinesterase inhibitor with a blocker of neurotoxicity induced by A beta in human brain causing Alzheimer's disease.  (+info)

(3/155) Host-plant diversity of the European corn borer Ostrinia nubilalis: what value for sustainable transgenic insecticidal Bt maize?

The strategies proposed for delaying the development of resistance to the Bacillus thuringiensis toxins produced by transgenic maize require high levels of gene flow between individuals feeding on transgenic and refuge plants. The European corn borer Ostrinia nubilalis (Hubner) may be found on several host plants, which may act as natural refuges. The genetic variability of samples collected on sagebrush (Artemisia sp.), hop (Humulus lupulus L.) and maize (Zea mays L.) was studied by comparing the allozyme frequencies for six polymorphic loci. We found a high level of gene flow within and between samples collected on the same host plant. The level of gene flow between the sagebrush and hop insect samples appeared to be sufficiently high for these populations to be considered a single genetic panmictic unit. Conversely, the samples collected on maize were genetically different from those collected on sagebrush and hop. Three of the six loci considered displayed greater between-host-plant than within-host-plant differentiation in comparisons of the group of samples collected on sagebrush or hop with the group of samples collected on maize. This indicates that either there is genetic isolation of the insects feeding on maize or that there is host-plant divergent selection at these three loci or at linked loci. These results have important implications for the potential sustainability of transgenic insecticidal maize.  (+info)

(4/155) Triterpenes and lignans from Artemisia caruifolia and their cytotoxic effects on meth-A and LLC tumor cell lines.

One new triterpene, 3beta-hydroxy-29-norcycloart-24-one (1), and four new lignans, caruilignans (2-5), together with six known compounds were isolated from the aerial part of Artemisia caruifolia BUCH.-HAM. ex TOXB. Their structures were determined by various spectroscopic means. Most of the isolated lignans were moderately cytotoxic to Meth-A cells with ED50 values of 5-10 microg/ml, but not to Lowis lung carcinoma (LLC) cells. An oxime derivative of 1 showed more potent cytotoxic activity against Meth-A and LLC cells than the original triterpene 1.  (+info)

(5/155) Inhibitory effects on HIV-1 protease of tri-p-coumaroylspermidine from Artemisia caruifolia and related amides.

From a methanol extract of Artemisia caruifolia, which showed a moderate inhibitory activity on HIV-1 protease in a preliminary screening, N1,N5,N10-tri-p-coumaroylspermidine and three dicaffeoylquinic acids were isolated. The former compound was found to appreciably inhibit HIV-1 protease. Of related amides which were chemically synthesized, N1,N5,N10,N14-tetra-p-coumaroylspermine and N1,N4,N7,N10,N13-penta-p-coumaroylte-traethylenepentamine inhibited HIV-1 protease more potently than N1,N5,N10-tri-p-coumaroylspermidine.  (+info)

(6/155) Antioxidant properties of natural compounds used in popular medicine for gastric ulcers.

There is evidence concerning the participation of reactive oxygen species in the etiology and physiopathology of human diseases, such as neurodegenerative disorders, inflammation, viral infections, autoimmune pathologies, and digestive system disorders such as gastrointestinal inflammation and gastric ulcer. The role of these reactive oxygen species in several diseases and the potential antioxidant protective effect of natural compounds on affected tissues are topics of high current interest. To consider a natural compound or a drug as an antioxidant substance it is necessary to investigate its antioxidant properties in vitro and then to evaluate its antioxidant functions in biological systems. In this review article, we shall consider the role of natural antioxidants derived from popular plants to reduce or prevent the oxidative stress in gastric ulcer induced by ethanol.  (+info)

(7/155) Fusion of farnesyldiphosphate synthase and epi-aristolochene synthase, a sesquiterpene cyclase involved in capsidiol biosynthesis in Nicotiana tabacum.

A clone encoding farnesyl diphosphate synthase (FPPS) was obtained by PCR from a cDNA library made from young leaves of Artemisia annua. A cDNA clone encoding the tobacco epi-aristolochene synthase (eAS) was kindly supplied by J. Chappell (University of Kentucky, Lexington, KY, USA). Two fusions were constructed, i.e. FPPS/eAS and eAS/FPPS. The stop codon of the N-terminal enzyme was removed and replaced by a short peptide (Gly-Ser-Gly) to introduce a linker between the two ORFs. These two fusions and the two single cDNA clones were separately introduced into a bacterial expression vector (pET32). Escherichia coli was transformed with the expression vectors and enzymatically active soluble proteins were obtained after induction with isopropyl thio-beta-d-thiogalactoside. The recombinant enzymes were purified using immobilized metal affinity chromatography on Co2+ columns. The fusion enzymes produced epi-aristolochene from isopentenyl diphosphate through a coupled reaction. The Km values of FPPS and eAS for isopentenyl diphosphate and farnesyl diphosphate, respectively, were essentially the same for the single and fused enzymes. The bifunctional enzymes showed a more efficient conversion of isopentenyl diphosphate to epi-aristolochene than the corresponding amount of single enzymes.  (+info)

(8/155) Influence of macronutrients and activated charcoal on intake of sagebrush by sheep and goats.

We determined if supplemental macronutrients or activated charcoal influenced intake of sagebrush, a shrub that contains terpenes. Sheep (lambs 3 mo age, 33 kg) and goats (kids 5 mo age, 28 kg) were fed supplements high in energy, high in protein, or offered a choice between the two supplements before and after receiving sagebrush for 4 h/d. The effect of activated charcoal, a compound that reduces bioavailability of toxins, was assessed by offering charcoal mixed with a carrier or the carrier alone while animals consumed sagebrush for 4 h/d. The effects of supplemental macronutirents were tested before and after charcoal supplementation. Activated charcoal did not increase intake of sagebrush by sheep or goats (P > 0.05), but supplemental macronutrients influenced intake of sagebrush by both species (P < 0.05). Intake of sage-brush was markedly higher for animals fed the high-protein supplement than for animals fed the high-energy supplement (P < 0.05). Lambs (before and after charcoal supplementation) and kids (after charcoal supplementation) offered a choice between the two supplements used sagebrush at levels comparable to animals supplemented with protein. They consumed more CP than animals fed the high-energy supplement and more ME than lambs fed the high-protein supplement (P < 0.05). Thus, providing a choice between supplements enhanced intake of sagebrush and enabled animals to achieve a more balanced intake of macronutrients. Preference for supplement and sagebrush also depended on an animal's immediate past history with a supplement. Sheep and goats previously fed the high-energy or the high-protein supplement preferred, respectively, the high-protein or the high-energy supplement when given a choice (P < 0.05). The changes in preference for supplement caused a decreased intake of sagebrush by groups that preferred the energy concentrate, and an increased intake of sagebrush by groups that preferred the protein concentrate. Thus, supplements of different energy and protein densities consistently altered intake of sagebrush by sheep and goats.  (+info)