*  Structural insight of DNA topoisomerases I from camptothecin-producing plants revealed by molecular dynamics simulations. -...
However, CPT-producing plants, including Camptotheca acuminata, Ophiorrhiza pumila and Ophiorrhiza liukiuensis, are highly ... However, CPT-producing plants, including Camptotheca acuminata, Ophiorrhiza pumila and Ophiorrhiza liukiuensis, are highly ...
  https://www.semanticscholar.org/paper/Structural-insight-of-DNA-topoisomerases-I-from-ca-Sirikantaramas-Meeprasert/6ff3c048374326c109ef18376c47afa5e9df4f9b
*  VackvSuG: Camptotheca checkpoint mechanism Baicalin wild type enzyme RPA-4 subunits, of RFC effects in vivo.
Camptotheca checkpoint mechanism Baicalin wild type enzyme RPA-4 subunits, of RFC effects in vivo. ... Camptotheca, Happy tree) or etoposide (VP-16) the topoisomerase inhibitors, this prevents DNA re-ligation and therefore causes ... which may signal the presence of DNA damage to an S-phase checkpoint mechanism isolated from the bark and stem of Camptotheca ... herbal medicine that has been commonly used as a clinical medicine it could inhibit the UVB-induced cytotoxicity of Camptotheca ...
  http://lnwme.blogspot.com/2009/09/camptotheca-checkpoint-mechanism.html
*  camptotheca | MTPR
In southern China, where Camptotheca acuminata is native, people call these big-leafed trees "Happy Trees." Chinese herbalists ...
  http://mtpr.org/term/camptotheca
*  Camptotheca - Wikipedia
Camptotheca acuminata Decne. Camptotheca lowreyana S.Y.Li The bark and stems of C. acuminata contain the alkaloid camptothecin ... Camptotheca (happy tree, cancer tree, or tree of life) is a genus of medium-sized deciduous trees growing to 20 metres (66 ft) ... and Hyperoside Isolated from Camptotheca acuminata". Journal of Agricultural and Food Chemistry. 53 (1): 32-37. doi:10.1021/ ...
  https://en.wikipedia.org/wiki/Camptotheca
*  VERY RARE Joe Hoak Variegated Agave Desmettiana Plant
Camptotheca Acuminata 700 Seeds, The Happy Tree, Tree Of Life, Cancer Tree. theplantattraction ...
  https://www.ecrater.com/p/2437099/very-rare-joe-hoak-variegated
*  Molecules | Free Full-Text | The Traditional Medicine and Modern Medicine from Natural Products | HTML
Camptotheca acuminata Decne. [1]. Camptothecin, irinotecan and topotecan. Antitumor. camptothecin. Gimkgo biloba L. [82]. ...
  http://www.mdpi.com/1420-3049/21/5/559/htm
*  Antimicrobial Compounds from Tree Endophytes | SpringerLink
Liu K, Ding X, Deng B et al (2010b) 10-Hydroxycamptothecin produced by a new endophytic Xylaria sp., M20, from Camptotheca ... Li S, Zhang Z, Cain A et al (2005) Antifungal activity of camptothecin, trifolin, and hyperoside isolated from Camptotheca ... I. The isolation and structure of camptothecin, a novel alkaloidal leukemia and tumor inhibitor from Camptotheca acuminata. J ... Kusari S, Zühlke S, Spiteller M (2009b) An endophytic fungus from Camptotheca acuminata that produces camptothecin and ...
  https://link.springer.com/chapter/10.1007/978-94-007-1599-8_17
*  Patent US6352996 - Liposomal prodrugs comprising derivatives of camptothecin and methods of ... - Google Patents
Camptothecin, a cytotoxic alkaloid first isolated from the wood and bark of Camptotheca Acuminata (Nyssaceae) by Wall and his ... Stabilized nanoparticle formulations of camptotheca derivatives. US20040101553 *. Aug 4, 2003. May 27, 2004. Transave, Inc.. ...
  http://www.google.com/patents/US6352996?dq=6,373,753
*  Patent US5059699 - Water soluble derivatives of taxol - Google Patents
Stabilized nanoparticle formulations of camptotheca derivatives. US20040018960 *. Jul 14, 2003. Jan 29, 2004. Pg-Txl Company, L ...
  http://www.google.com/patents/US5059699?dq=4453763
*  The 3-hydroxy-3-methylglutaryl coenzyme-A (HMG-CoA) reductases | Springer for Research & Development
The enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase catalyzes the conversion of HMG-CoA to mevalonate, a four-electron oxidoreduction that is the rate-limiting step in the synthesis...
  https://rd.springer.com/article/10.1186/gb-2004-5-11-248
*  Patent US6455699 - Camptothecin analogs and methods of preparation thereof - Google Patents
of a Chinese plant, Camptotheca accuminata, by Wall. Wall, M. E. et al, J. Am. Chem. Soc., 88, 3888 (1966). As depicted below, ...
  http://www.google.com/patents/US6455699?dq=U.S.+Patent+No.+4,528,643
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Isolation and characterization of endophytic fungi from Camptotheca acuminata.. Xiaowei Ding, Kaihui Liu, Baiwan Deng, Wenqiang ... In this study, a total of 161 endophytic fungal isolates from Camptotheca acuminata were obtained and classified to 16 taxa ... A new 10-hydroxycamptothecin (HCPT)-producing fungus was isolated from Camptotheca acuminata. The strain was classified as a ... 10-Hydroxycamptothecin produced by a new endophytic Xylaria sp., M20, from Camptotheca acuminata. ...
  https://www.jove.com/visualize?author=Kaihui%20Hu%20He%20
*  7-Ethyl-10-hydroxycamptothecin 86639-52-3 properties reference
Hydroxy Camptothecine; SN38; CAMPTOTHECIN, 10-HYDROXY-; CAMPTOTHECIN, 10-HYDROXY-, CAMPTOTHECA ACUMINATA; HCPT; ... CAMPTOTHECIN, 10-HYDROXY-; CAMPTOTHECIN, 10-HYDROXY-, CAMPTOTHECA ACUMINATA; HCPT; HYDROXYCAMPTOTHECIN; HYDROXYCAMPTOTHECIN, 10 ...
  http://www.guidechem.com/reference/dic-188221.html
*  Cornales
2016) obtained the relationships [Diplopanax + Mastixia] [Davidia [Camptotheca + Nyssa]]], with good support. ...
  http://www.mobot.org/mobot/research/apweb/orders/cornalesweb.htm
*  Taxol - Chemotherapy Drugs - Chemocare
Camptothecan analogs are derived from the Asian "Happy Tree" (Camptotheca acuminata). Podophyllotoxins and camptothecan analogs ...
  http://www.chemocare.com/chemotherapy/drug-info/Taxol.aspx
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
nov., isolated from rhizosphere soil of Camptotheca acuminata Decne.. Lei Shen, Long-Peng Zheng, Huan Liu, Rui Liu, Ke-Yun ... Strain LXD30(T) was isolated from rhizosphere soil of a plant of the species Camptotheca acuminata Decne which is native to ...
  https://www.jove.com/visualize?author=Ren-Hau+Lai
*  Plant Photo Index
Nyssaceae Camptotheca acuminata 264 Japan Tokyo 1999/7/19 Chinese origin, original plant of campthotecine Nyssaceae Camptotheca ...
  http://home.hiroshima-u.ac.jp/shoyaku/PlantFamily2002.htm
*  Agriculture | Free Full-Text | An Alternative Use of Horticultural Crops: Stressed Plants as Biofactories of Bioactive...
Kim, Y.J.; Kim, D.G.; Lee, S.H.; Lee, I. Wound-induced expression of the ferulate 5-hydroxylase gene in Camptotheca acuminata. ... Likewise, Kim et al. [37] determined that treating wounded Camptotheca acuminata tissue with AVG does not affect the gene ... and Camptotheca acuminata, respectively. Choi et al. [38] exposed wounded-lettuce tissue to vapors (20 μmol/g FW) or aqueous ... carrots and Camptotheca acuminata. Saltveit [36] reported that the use of 1-MCP before or after the application of wounding ...
  http://www.mdpi.com/2077-0472/2/3/259/htm
*  Patent US4031098 - Camptothecin analogues - Google Patents
Camptothecin is an alkaloid isolated from the stem wood of the Chinese tree, Camptotheca acuminata Decsne.(Nyssaceae), of which ...
  http://www.google.com/patents/US4031098?dq=artistshare
*  Medicinal Plant Genomics Resource
The plants utilized by MPGR are Atropa belladonna, Camptotheca acuminata, Cannabis sativa, Catharanthus roseus, Digitalis ... Genome sequence of Camptotheca acuminata. We have generated the genome sequence of Camptotheca acuminata also known as the " ... Analysis of the Camptotheca acuminata, Catharanthus roseus, and Rauvolfia serpentina transcriptomes are described in PLoS ONE ... We have released the genome of Camptotheca acuminata as described in Zhao et al. 2017. See the genome browser, BLAST search ...
  http://www.medicinalplantgenomics.msu.edu

(1/13) Wound-induced expression of the ferulate 5-hydroxylase gene in Camptotheca acuminata.

The phenylpropanoid pathway plays important roles in plants following exposure to environmental stresses, such as wounding and pathogen attack, which lead to the production of a variety of compounds, including lignin, flavonoids and phytoalexins. Ferulate 5-hydroxylase (F5H) is a cytochrome P450-dependent monooxygenase that catalyses the hydroxylation of ferulic acid, coniferaldehyde and coniferyl alcohol, leading to sinapic acid and syringyl lignin biosynthesis. We isolated F5H cDNA and genomic DNA from Camptotheca acuminata and investigated the expression pattern of the C. acuminata F5H (CaF5H1) gene in response to wounding. A search against the BLOCKS database of conserved protein motifs indicated that CaF5H1 retains features in common with F5Hs reported from other plants. 5'-flanking region analysis using the PLACE database showed that putative regulatory elements related to various abiotic and biotic stresses, such as drought, wounding, low temperature and pathogens, exist in the 5'-flanking region of CaF5H1. Based upon these analysis results, we investigated the expression pattern of CaF5H1 gene in response to wounding and stress-related molecules. Here, we show that CaF5H1 transcripts accumulated in the leaves in response to mechanical wounding or the application of molecules involved in the stress response, such as ethylene, ABA and hydrogen peroxide (H2O2). The application of salicylic acid and diphenylene iodonium (DPI) inhibited the wound-induced expression of CaF5H1. Taken together, we suggest that wound-induced expression of CaF5H1 may be mediated by MJ and H2O2 and enhanced phenylpropanoid contents via CaF5H1 maybe function in response to various stresses, including wounding, in plants.  (+info)

(2/13) Molecular cloning and characterization of a novel stem-specific gene from Camptotheca acuminata.

In higher plants, P450s participate in the biosynthesis of many important secondary metabolites. Here we reported for the first time the isolation of a new cytochrome P450 cDNA that expressed in a stem-specific manner from Camptotheca acuminata (designated as CaSS), a native medicinal plant species in China, using RACE-PCR. The full-length cDNA of CaSS was 1735 bp long containing a 1530 bp open reading frame (ORF) encoding a polypeptide of 509 amino acids. Bioinformatic analysis revealed that CASS contained a heme-binding domain PFGXGRRXCX and showed homology to other plant cytochrome P450 monooxygenases and hydroxylases. Southern blotting analysis revealed that there was only one copy of the CaSS present in the genome of Camptotheca acuminata. Northern blotting analysis revealed that CaSS expressed, in a tissuespecific manner, highly in stem and lowly in root, leaf and flower. Our study suggests that CaSS is likely to be involved in the phenylpropanoid pathway.  (+info)

(3/13) Total synthesis and biological evaluation of 22-hydroxyacuminatine.

A total synthesis of 22-hydroxyacuminatine, a cytotoxic alkaloid isolated from Camptotheca acuminata, is reported. The key step in the synthesis involves the reaction of 2,3-dihydro-1H-pyrrolo[3,4-b]quinoline with a brominated phthalide to generate a substituted pentacyclic 12H-5,11a-diazadibenzo[b,h]fluoren-11-one intermediate. Despite its structural resemblance to camptothecin and luotonin A, a biological evaluation of 22-hydroxyacuminatine in a topoisomerase I-deficient cell line P388/CPT45 has confirmed that the observed cytotoxicity is not due to topoisomerase I inhibition, even though 22-hydroxyacuminatine has a hydroxyl group that can theoretically hydrogen bond to Asp533. This result is consistent with the hypothesis that pi-pi stacking is more important than hydrogen-bonding interactions in determining topoisomerase I inhibitor binding in the ternary cleavage complex.  (+info)

(4/13) Molecular cloning and characterization of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (CaHDR) from Camptotheca acuminata and its functional identification in Escherichia coli.

Camptothecin is an anti-cancer monoterpene indole alkaloid. The gene encoding 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (designated as CaHDR), the last catalytic enzyme of the MEP pathway for terpenoid biosynthesis, was isolated from camptothecin-producing Camptotheca acuminata. The full-length cDNA of CaHDR was 1686 bp encoding 459 amino acids. Comparison of the cDNA and genomic DNA of CaHDR revealed that there was no intron in genomic CaHDR. Southern blot analysis indicated that CaHDR belonged to a low-copy gene family. RT-PCR analysis revealed that CaHDR expressed constitutively in all tested plant organs with the highest expression level in flowers, and the expression of CaHDR could be induced by 100 microM methyl-jasmonate (MeJA), but not by 100 mg/L salicylic acid (SA) in the callus of C. acuminata. The complementation of CaHDR in Escherichia coli ispH mutant MG1655 demonstrated its function.  (+info)

(5/13) Molecular cloning, characterization and expression of a jasmonate biosynthetic pathway gene encoding allene oxide cyclase from Camptotheca acuminata.

 (+info)

(6/13) Cell-specific expression of tryptophan decarboxylase and 10-hydroxygeraniol oxidoreductase, key genes involved in camptothecin biosynthesis in Camptotheca acuminata Decne (Nyssaceae).

 (+info)

(7/13) Rhizobium kunmingense sp. nov., isolated from rhizosphere soil of Camptotheca acuminata Decne.

Strain LXD30(T) was isolated from rhizosphere soil of a plant of the species Camptotheca acuminata Decne which is native to warm, humid stream banks in southern China. Analysis of the 16S rRNA gene sequence revealed that the Gram-negative, rod-shaped bacterium fell within the realm of the genus Rhizobium and was most closely related to Rhizobium huautlense SO2(T) (96.4% sequence similarity) and Rhizobium cellulosilyticum LMG 23642(T) (96.4%). The isolate grew optimally at pH7.0 and 25-28 degrees C in the presence of 0-1% (w/v) NaCl. Major fatty acids were C16:0 (17.5%) and summed feature 7 (C18:1omega7c/omega9t/omega12t, 58.3%). Unequivocally low 16S rRNA (<97%), recA (<92%) and atpD (<90%) gene sequence similarities to all existing species of the genus and phenotypic characteristics all suggested that strain LXD30(T) (=KCTC 22609(T)=CGMCC 1.8903(T)) represents a novel Rhizobium species, for which the name Rhizobium kunmingense sp. nov. is proposed.  (+info)

(8/13) Evaluation of antimicrobial activity of endophytic fungi from Camptotheca acuminata (Nyssaceae).

 (+info)



  • cancer
  • There can be many years between promising laboratory work and the availability of an effective anti-cancer drug: Monroe Eliot Wall discovered anti-cancer properties in Camptotheca in 1958, but it was not until 1996 - after further research and rounds of clinical trials - that topotecan, a synthetic derivative of a chemical in the plant, was approved for use by the US Food and Drug Administration. (wikipedia.org)