A family of BACTERIOPHAGES and ARCHAEAL VIRUSES which are characterized by long, non-contractile tails.
Viruses whose host is Streptococcus.
A family of BACTERIOPHAGES and ARCHAEAL VIRUSES which are characterized by complex contractile tails.
An order comprising three families of tailed bacteriophages: MYOVIRIDAE; PODOVIRIDAE; and SIPHOVIRIDAE.
Genomes of temperate BACTERIOPHAGES integrated into the DNA of their bacterial host cell. The prophages can be duplicated for many cell generations until some stimulus induces its activation and virulence.
Viruses whose hosts are bacterial cells.
The complete genetic complement contained in a DNA or RNA molecule in a virus.
Proteins found in the tail sections of DNA and RNA viruses. It is believed that these proteins play a role in directing chain folding and assembly of polypeptide chains.
A family of bacteriophages which are characterized by short, non-contractile tails.
A genus of gram-positive, coccoid bacteria mainly isolated from milk and milk products. These bacteria are also found in plants and nonsterile frozen and dry foods. Previously thought to be a member of the genus STREPTOCOCCUS (group N), it is now recognized as a separate genus.
The phenomenon by which a temperate phage incorporates itself into the DNA of a bacterial host, establishing a kind of symbiotic relation between PROPHAGE and bacterium which results in the perpetuation of the prophage in all the descendants of the bacterium. Upon induction (VIRUS ACTIVATION) by various agents, such as ultraviolet radiation, the phage is released, which then becomes virulent and lyses the bacterium.
The properties of a pathogen that makes it capable of infecting one or more specific hosts. The pathogen can include PARASITES as well as VIRUSES; BACTERIA; FUNGI; or PLANTS.
A non-pathogenic species of LACTOCOCCUS found in DAIRY PRODUCTS and responsible for the souring of MILK and the production of LACTIC ACID.
Viruses whose host is Staphylococcus.
A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).
The sequential location of genes on a chromosome.
Electron microscopy in which the ELECTRONS or their reaction products that pass down through the specimen are imaged below the plane of the specimen.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Deoxyribonucleic acid that makes up the genetic material of viruses.
Proteins found in any species of virus.
The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.
The relationships of groups of organisms as reflected by their genetic makeup.

The complete genome sequence of the Streptomyces temperate phage straight phiC31: evolutionary relationships to other viruses. (1/140)

The completed genome sequence of the temperate Streptomyces phage straight phiC31 is reported. straight phiC31 contains genes that are related by sequence similarities to several other dsDNA phages infecting many diverse bacterial hosts, including Escherichia, Arthrobacter, Mycobacterium, Rhodobacter, Staphylococcus, Bacillus, Streptococcus, Lactobacillus and Lactococcus. These observations provide further evidence that dsDNA phages from diverse bacterial hosts are related and have had access to a common genetic pool. Analysis of the late genes was particularly informative. The sequences of the head assembly proteins (portal, head protease and major capsid) were conserved between straight phiC31, coliphage HK97, staphylococcal phage straight phiPVL, two Rhodobacter capsulatus prophages and two Mycobacterium tuberculosis prophages. These phages and prophages (where non-defective) from evolutionarily diverse hosts are, therefore, likely to share a common head assembly mechanism i.e. that of HK97. The organisation of the tail genes in straight phiC31 is highly reminiscent of tail regions from other phage genomes. The unusual organisation of the putative lysis genes in straight phiC31 is discussed, and speculations are made as to the roles of some inessential early gene products. Similarities between certain phage gene products and eukaryotic dsDNA virus proteins were noted, in particular, the primase/helicases and the terminases (large subunits). Furthermore, the complete sequence clarifies the overall transcription map of the phage during lytic growth and the positions of elements involved in the maintenance of lysogeny.  (+info)

Genomic structure of phage B40-8 of Bacteroides fragilis. (2/140)

Very few data are available on the molecular biology of Bacteroides fragilis bacteriophages, which have been considered in several studies as indicators of faecal contamination. Phage B40-8, initially isolated from an urban sewage sample using a strain of B. fragilis (HSP40) isolated from a clinical specimen, was chosen in this study as a prototype for morphological and molecular studies. Like most of the phages infective for B. fragilis, B40-8 belongs to the Siphoviridae family. Its genome has been found to be a double-stranded DNA molecule, of approximately 51.7 kb, containing a rather low percentage (38.9 mol%) of G + C. The ends of the molecule appeared not to be cohesive but permuted, with a terminal redundancy of 7.3%. A genomic map was constructed. Three major proteins (MP) out of 15 peptides in the SDS-PAGE profile were selected for N-terminal sequencing. From these data, degenerate probes were designed to locate the ORFs in the genomic map. Immunodetection by electron microscopy revealed that MP1 and MP3 were structural proteins of the phage head and that MP2 was a constituent of the tail. A genomic library of the phage was prepared, and a clone including the MP2 ORF was identified and sequenced.  (+info)

Protective effects of bacteriophage on experimental Lactococcus garvieae infection in yellowtail. (3/140)

The present study describes the in vitro and in vivo survival of Lactococcus garvieae bacteriophages and the potential of the phage for controlling experimental L. garvieae infection in yellowtail. Anti-L. garvieae phages persisted well in various physicochemical (water temperature, salinity, pH) and biological (feed, serum and alimentary tract extracts of yellowtail) conditions, except for low acidity. In the in vivo, the phage PLgY-16 was detected in the spleens of yellowtail until 24 h after intraperitoneal (i.p.) injection, or the phage was recovered from the intestine of yellowtail 3 h after the oral administration of phage-impregnated feed but undetectable 10 h later. Simultaneous administration of live L. garvieae and phage enhanced recovery of the phage from the spleen or intestine. The survival rate was much higher in yellowtail that received i.p. injection of the phage after i.p. challenge with L. garvieae, compared with that of control fish without phage injection. When fish were i.p. injected with phage at different hours after L. garvieae challenge, higher protective effects were demonstrated in fish that received phage treatment at the earlier time. Protection was also obtained in yellowtail receiving phage-impregnated feed, in which fish were challenged by an anal intubation with L. garvieae. Anal-intubated L. garvieae were detected constantly in the spleens of the control fish, while they were detected sporadically and disappeared from the phage-treated fish 48 h later. On the other hand, orally administered phage was detected at high plaque-forming units from the intestines and spleens of the phage-treated fish until 48 h later. These results indicate that intraperitoneally or orally administered anti-L. garvieae phage prevented fish from experimental L. garvieae infection, suggesting potential use of the phage for controlling the disease.  (+info)

Similarly organized lysogeny modules in temperate Siphoviridae from low GC content gram-positive bacteria. (4/140)

Temperate Siphoviridae from an evolutionarily related branch of low GC content gram-positive bacteria share a common genetic organization of lysogeny-related genes and the predicted proteins are linked by many sequence similarities. Their compact lysogeny modules [integrase/1-2 orfs (phage exclusion? and metalloproteinase motif proteins)/cI-like repressor/cro-like repressor/antirepressor (optional)] differ clearly from that of lambda-like and L5-like viruses, the two currently established genera of temperate Siphoviridae, while they resemble those of the P2-like genus of Myoviridae. In all known temperate Siphoviridae from low GC content gram-positive bacteria the lysogeny module is flanked by the lysis module and the DNA replication module. This modular organization is again distinct from that of the known genera of temperate Siphoviridae. On the basis of comparative sequence analysis we propose a new genus of Siphoviridae: "Sfi21-like" phages. With a larger database of phage sequences it might be possible to establish a genomics-based phage taxonomy and to retrace the evolutionary history of selected phage modules or individual phage genes. The antirepressor of Sfi21-like phages has an unusual widespread distribution since proteins with high aa similarity (40%) were found not only in phages from gram-negative bacteria, but also in insect viruses.  (+info)

Comparative genomics of the late gene cluster from Lactobacillus phages. (5/140)

Three prophage sequences were identified in the Lactobacillus johnsoni strain NCC533. Prophage Lj965 predicted a gene map very similar to those of pac-site Streptococcus thermophilus phages over its DNA packaging and head and tail morphogenesis modules. Sequence similarity linked the putative DNA packaging and head morphogenesis genes at the protein level. Prophage Lj965/S. thermophilus phage Sfi11/Lactococcus lactis phage TP901-1 on one hand and Lactobacillus delbrueckii phage LL-H/Lactobacillus plantarum phage phig1e/Listeria monocytogenes phage A118 on the other hand defined two sublines of structural gene clusters in pac-site Siphoviridae from low-GC Gram-positive bacteria. Bacillus subtilis phage SPP1 linked both sublines. The putative major head and tail proteins from Lj965 shared weak sequence similarity with phages from Gram-negative bacteria. A clearly independent line of structural genes in Siphoviridae from low-GC Gram-positive bacteria is defined by temperate cos-site phages including Lactobacillus gasseri phage adh, which also shared sequence similarity with phage D3 infecting a Gram-negative bacterium. A phylogenetic tree analysis demonstrated that the ClpP-like protein identified in four cos-site Siphoviridae from Lactobacillus, Lactococcus, Streptococcus, and Pseudomonas showed graded sequence relationships. The tree suggested that the ClpP-like proteins from the phages were not acquired by horizontal gene transfer from their corresponding bacterial hosts.  (+info)

Topologically linked protein rings in the bacteriophage HK97 capsid. (6/140)

The crystal structure of the double-stranded DNA bacteriophage HK97 mature empty capsid was determined at 3.6 angstrom resolution. The 660 angstrom diameter icosahedral particle contains 420 subunits with a new fold. The final capsid maturation step is an autocatalytic reaction that creates 420 isopeptide bonds between proteins. Each subunit is joined to two of its neighbors by ligation of the side-chain lysine 169 to asparagine 356. This generates 12 pentameric and 60 hexameric rings of covalently joined subunits that loop through each other, creating protein chainmail: topologically linked protein catenanes arranged with icosahedral symmetry. Catenanes have not been previously observed in proteins and provide a stabilization mechanism for the very thin HK97 capsid.  (+info)

Sequence of the genome of the temperate, serotype-converting, Pseudomonas aeruginosa bacteriophage D3. (7/140)

Temperate bacteriophage D3, a member of the virus family Siphoviridae, is responsible for serotype conversion in its host, Pseudomonas aeruginosa. The complete sequence of the double-stranded DNA genome has been determined. The 56,426 bp contains 90 putative open reading frames (ORFs) and four genes specifying tRNAs. The latter are specific for methionine (AUG), glycine (GGA), asparagine (AAC), and threonine (ACA). The tRNAs may function in the translation of certain highly expressed proteins from this relatively AT-rich genome. D3 proteins which exhibited a high degree of sequence similarity to previously characterized phage proteins included the portal, major head, tail, and tail tape measure proteins, endolysin, integrase, helicase, and NinG. The layout of genes was reminiscent of lambdoid phages, with the exception of the placement of the endolysin gene, which parenthetically also lacked a cognate holin. The greatest sequence similarity was found in the morphogenesis genes to coliphages HK022 and HK97. Among the ORFs was discovered the gene encoding the fucosamine O-acetylase, which is in part responsible for the serotype conversion events.  (+info)

Mutational analysis of two structural genes of the temperate lactococcal bacteriophage TP901-1 involved in tail length determination and baseplate assembly. (8/140)

Two putative structural genes, orf tmp (tape measure protein) and orf bpp (baseplate protein), of the temperate lactococcal phage TP901-1 were examined by introduction of specific mutations in the prophage strain Lactococcus lactic ssp. cremoris 901-1. The adsorption efficiencies of the mutated phages to the indicator strain L. lactic ssp. cremoris 3107 were determined and electron micrographs were obtained. Specific mutations in orf tmp resulted in the production of mostly phage head structures without tails and a few wild-type looking phages. Furthermore, construction of an inframe deletion or duplication of 29% in orf tmp was shown to shorten or lengthen the phage tail by approximately 30%, respectively. The orf tmp is proposed to function as a tape measure protein, TMP, important for assembly of the TP901-1 phage tail and involved in tail length determination. Specific mutations in orf bpp produced phages which were unable to adsorb to the indicator strain and electron microscopy revealed particles lacking the baseplate structure. The orf bpp is proposed to encode a highly immunogenic structural baseplate protein, BPP, important for assembly of the baseplate. Finally, an assembly pathway of the TP901-1 tail and baseplate structure is presented.  (+info)

Siphoviridae is a family of tailed bacteriophages, which are viruses that infect and replicate within bacteria. The members of this family are characterized by their long, non-contractile tails, which are typically around 100-1000 nanometers in length. The tail fibers at the end of the tail are used to recognize and attach to specific receptors on the surface of bacterial cells.

The Siphoviridae family includes many well-known bacteriophages, such as the lambda phage that infects Escherichia coli bacteria. The genetic material of Siphoviridae viruses is double-stranded DNA, which is packaged inside an icosahedral capsid (the protein shell of the virus).

It's worth noting that Siphoviridae is one of the five families in the order Caudovirales, which includes all tailed bacteriophages. The other four families are Myoviridae, Podoviridae, Herelleviridae, and Ackermannviridae.

I'm sorry for any confusion, but "Streptococcus Phages" is not a standard medical term. The term "Streptococcus" refers to a genus of gram-positive bacteria that can cause various infections in humans, such as strep throat and skin infections. A "phage," on the other hand, is a virus that infects and replicates within bacteria.

Therefore, if you are referring to bacteriophages that infect Streptococcus bacteria, then the term would be "Streptococcus phages" or more specifically, the name of the particular phage species that infect Streptococcus bacteria (e.g., Streptococcus phage C1, Streptococcus phage Φ29). However, it's important to note that there are many different types of bacteriophages that can infect various strains of Streptococcus bacteria, so the specific phage would need to be identified.

Myoviridae is a family of bacteriophages, which are viruses that infect and replicate within bacteria. Here is the medical definition of Myoviridae:

Myoviridae is a family of tailed bacteriophages characterized by a contractile sheath surrounding the tail structure. The members of this family have a double-stranded DNA (dsDNA) genome, which is relatively large, ranging from 40 to over 200 kilobases in size. Myoviridae viruses typically infect Gram-negative bacteria and are known to cause lysis of the host cell upon replication. The family includes many well-known bacteriophages such as T4, T5, and λ phages, which have been extensively studied for their biological properties and potential applications in molecular biology and medicine.

It's worth noting that while Myoviridae viruses can be useful tools in scientific research, they are not used in clinical practice as therapeutic agents. However, there is ongoing research into the use of bacteriophages, including those from the family Myoviridae, for the treatment of bacterial infections that are resistant to antibiotics.

Caudovirales is an order of viruses that includes tailed bacteriophages, which are viruses that infect and replicate within bacteria. The name "Caudovirales" is derived from the Latin word "cauda," meaning tail, and refers to the characteristic tail structure present on these viruses.

The members of Caudovirales have a complex virion structure, consisting of an icosahedral capsid that contains the viral genome, and a tail structure that is used for attachment to and infection of the host bacterial cell. The tail structure typically consists of a contractile sheath surrounding a core containing tail fibers or spikes, which recognize and bind to specific receptors on the surface of the host cell.

The genome of Caudovirales members is usually double-stranded DNA (dsDNA), although some members have single-stranded DNA (ssDNA) genomes. The genome size can vary widely, ranging from around 10 to over 200 kilobases in length.

Caudovirales viruses are ubiquitous in the environment and play important roles in shaping bacterial communities and ecology. They have been studied extensively as models for understanding virus-host interactions and have potential applications in biotechnology and medicine, such as phage therapy for treating bacterial infections.

A prophage is a bacteriophage (a virus that infects bacteria) genome that is integrated into the chromosome of a bacterium and replicates along with it. The phage genome remains dormant within the bacterial host until an environmental trigger, such as stress or damage to the host cell, induces the prophage to excise itself from the bacterial chromosome and enter a lytic cycle, during which new virions are produced and released by lysing the host cell. This process is known as lysogeny.

Prophages can play important roles in the biology of their bacterial hosts, such as contributing to genetic diversity through horizontal gene transfer, modulating bacterial virulence, and providing resistance to superinfection by other phages. However, they can also have detrimental effects on the host, such as causing lysis or altering bacterial phenotypes in ways that are disadvantageous for survival.

It's worth noting that not all bacteriophages form prophages; some exist exclusively as extrachromosomal elements, while others can integrate into the host genome but do not necessarily become dormant or replicate with the host cell.

Bacteriophages, often simply called phages, are viruses that infect and replicate within bacteria. They consist of a protein coat, called the capsid, that encases the genetic material, which can be either DNA or RNA. Bacteriophages are highly specific, meaning they only infect certain types of bacteria, and they reproduce by hijacking the bacterial cell's machinery to produce more viruses.

Once a phage infects a bacterium, it can either replicate its genetic material and create new phages (lytic cycle), or integrate its genetic material into the bacterial chromosome and replicate along with the bacterium (lysogenic cycle). In the lytic cycle, the newly formed phages are released by lysing, or breaking open, the bacterial cell.

Bacteriophages play a crucial role in shaping microbial communities and have been studied as potential alternatives to antibiotics for treating bacterial infections.

A viral genome is the genetic material (DNA or RNA) that is present in a virus. It contains all the genetic information that a virus needs to replicate itself and infect its host. The size and complexity of viral genomes can vary greatly, ranging from a few thousand bases to hundreds of thousands of bases. Some viruses have linear genomes, while others have circular genomes. The genome of a virus also contains the information necessary for the virus to hijack the host cell's machinery and use it to produce new copies of the virus. Understanding the genetic makeup of viruses is important for developing vaccines and antiviral treatments.

I'm sorry for any confusion, but "viral tail proteins" is not a widely recognized or established medical term. The term "tail proteins" is used in the context of certain viruses, particularly bacteriophages (viruses that infect bacteria), which have a tail-like structure that helps them attach to and inject their genetic material into host cells.

However, even within this context, there isn't a specific concept known as "viral tail proteins" that has a widely accepted medical definition. The proteins that make up the tail structure of bacteriophages have various functions and are referred to by different names based on their roles. These can include terms like "tail fiber proteins," "tail tube proteins," "tail terminator proteins," etc.

If you're looking for information about a specific protein or group of proteins related to viral tails, I would be happy to help further if you could provide more details.

Podoviridae is a family of viruses in the order Caudovirales, which are tailed, double-stranded DNA viruses. The members of this family are characterized by their short, noncontractile tails. The virions (virus particles) of Podoviridae are typically icosahedral in shape and measure around 60 nanometers in diameter.

The host organisms of Podoviridae are primarily bacteria, making them bacteriophages or phages. They infect and replicate within the host bacterium, often leading to its lysis (breakdown) and release of new virions. The family Podoviridae is further divided into several genera, including T7-like viruses, N4-like viruses, and P22-like viruses, among others.

It's worth noting that while Podoviridae is a well-established family of bacteriophages, the field of virology is constantly evolving as new research and discoveries are made. Therefore, it's possible that the classification and definition of Podoviridae may change over time.

Lactococcus is a genus of Gram-positive, facultatively anaerobic bacteria commonly found in plants, dairy products, and the oral and intestinal microbiota of animals and humans. These bacteria are known for their ability to ferment lactose and other sugars into lactic acid, which makes them important in food production (such as cheese and buttermilk) and also contributes to their role in dental caries. Some species of Lactococcus can cause disease in humans, particularly in immunocompromised individuals or those with pre-existing conditions, but they are generally considered to be low-virulence pathogens.

Lysogeny is a process in the life cycle of certain viruses, known as bacteriophages or phages, which can infect bacteria. In lysogeny, the viral DNA integrates into the chromosome of the host bacterium and replicates along with it, remaining dormant and not producing any new virus particles. This state is called lysogeny or the lysogenic cycle.

The integrated viral DNA is known as a prophage. The bacterial cell that contains a prophage is called a lysogen. The lysogen can continue to grow and divide normally, passing the prophage onto its daughter cells during reproduction. This dormant state can last for many generations of the host bacterium.

However, under certain conditions such as DNA damage or exposure to UV radiation, the prophage can be induced to excise itself from the bacterial chromosome and enter the lytic cycle. In the lytic cycle, the viral DNA replicates rapidly, producing many new virus particles, which eventually leads to the lysis (breaking open) of the host cell and the release of the newly formed virions.

Lysogeny is an important mechanism for the spread and survival of bacteriophages in bacterial populations. It also plays a role in horizontal gene transfer between bacteria, as genes carried by prophages can be transferred to other bacteria during transduction.

Host specificity, in the context of medical and infectious diseases, refers to the tendency of a pathogen (such as a virus, bacterium, or parasite) to infect and cause disease only in specific host species or individuals with certain genetic characteristics. This means that the pathogen is not able to establish infection or cause illness in other types of hosts. Host specificity can be determined by various factors such as the ability of the pathogen to attach to and enter host cells, replicate within the host, evade the host's immune response, and obtain necessary nutrients from the host. Understanding host specificity is important for developing effective strategies to prevent and control infectious diseases.

"Lactococcus lactis" is a species of gram-positive, facultatively anaerobic bacteria that are commonly found in nature, particularly in environments involving plants and dairy products. It is a catalase-negative, non-spore forming coccus that typically occurs in pairs or short chains.

"Lactococcus lactis" has significant industrial importance as it plays a crucial role in the production of fermented foods such as cheese and buttermilk. The bacterium converts lactose into lactic acid, which contributes to the sour taste and preservative qualities of these products.

In addition to its use in food production, "Lactococcus lactis" has been explored for its potential therapeutic applications. It can be used as a vector for delivering therapeutic proteins or vaccines to the gastrointestinal tract due to its ability to survive and colonize there.

It's worth noting that "Lactococcus lactis" is generally considered safe for human consumption, and it's one of the most commonly used probiotics in food and supplements.

Staphylococcus phages do not have a specific medical definition, but I can provide you with some information about both concepts:

1. Staphylococcus: This term refers to a group of bacteria that can cause various infections in humans and animals. The most common species is Staphylococcus aureus, which often colonizes the skin and nasal passages of healthy individuals. However, it can lead to infections when it enters the body through wounds or other breaks in the skin.

2. Phages: These are viruses that infect and kill bacteria. They specifically target and replicate within bacterial cells, using the host's machinery for their reproduction. Once the phage has multiplied sufficiently, it causes the bacterial cell to lyse (burst), releasing new phage particles into the environment. Phages can be specific to certain bacterial species or strains, making them potential alternatives to antibiotics in treating bacterial infections without disrupting the normal microbiota.

When combining these two concepts, Staphylococcus phages refer to viruses that infect and kill Staphylococcus bacteria. These phages can be used as therapeutic agents to treat Staphylococcus infections, particularly those caused by antibiotic-resistant strains like methicillin-resistant Staphylococcus aureus (MRSA). However, it is essential to note that the use of phages as a treatment option is still an experimental approach and requires further research before becoming a widely accepted therapeutic strategy.

An open reading frame (ORF) is a continuous stretch of DNA or RNA sequence that has the potential to be translated into a protein. It begins with a start codon (usually "ATG" in DNA, which corresponds to "AUG" in RNA) and ends with a stop codon ("TAA", "TAG", or "TGA" in DNA; "UAA", "UAG", or "UGA" in RNA). The sequence between these two points is called a coding sequence (CDS), which, when transcribed into mRNA and translated into amino acids, forms a polypeptide chain.

In eukaryotic cells, ORFs can be located in either protein-coding genes or non-coding regions of the genome. In prokaryotic cells, multiple ORFs may be present on a single strand of DNA, often organized into operons that are transcribed together as a single mRNA molecule.

It's important to note that not all ORFs necessarily represent functional proteins; some may be pseudogenes or result from errors in genome annotation. Therefore, additional experimental evidence is typically required to confirm the expression and functionality of a given ORF.

Gene order, in the context of genetics and genomics, refers to the specific sequence or arrangement of genes along a chromosome. The order of genes on a chromosome is not random, but rather, it is highly conserved across species and is often used as a tool for studying evolutionary relationships between organisms.

The study of gene order has also provided valuable insights into genome organization, function, and regulation. For example, the clustering of genes that are involved in specific pathways or functions can provide information about how those pathways or functions have evolved over time. Similarly, the spatial arrangement of genes relative to each other can influence their expression levels and patterns, which can have important consequences for phenotypic traits.

Overall, gene order is an important aspect of genome biology that continues to be a focus of research in fields such as genomics, genetics, evolutionary biology, and bioinformatics.

Transmission electron microscopy (TEM) is a type of microscopy in which an electron beam is transmitted through a ultra-thin specimen, interacting with it as it passes through. An image is formed from the interaction of the electrons with the specimen; the image is then magnified and visualized on a fluorescent screen or recorded on an electronic detector (or photographic film in older models).

TEM can provide high-resolution, high-magnification images that can reveal the internal structure of specimens including cells, viruses, and even molecules. It is widely used in biological and materials science research to investigate the ultrastructure of cells, tissues and materials. In medicine, TEM is used for diagnostic purposes in fields such as virology and bacteriology.

It's important to note that preparing a sample for TEM is a complex process, requiring specialized techniques to create thin (50-100 nm) specimens. These include cutting ultrathin sections of embedded samples using an ultramicrotome, staining with heavy metal salts, and positive staining or negative staining methods.

Molecular sequence data refers to the specific arrangement of molecules, most commonly nucleotides in DNA or RNA, or amino acids in proteins, that make up a biological macromolecule. This data is generated through laboratory techniques such as sequencing, and provides information about the exact order of the constituent molecules. This data is crucial in various fields of biology, including genetics, evolution, and molecular biology, allowing for comparisons between different organisms, identification of genetic variations, and studies of gene function and regulation.

DNA Sequence Analysis is the systematic determination of the order of nucleotides in a DNA molecule. It is a critical component of modern molecular biology, genetics, and genetic engineering. The process involves determining the exact order of the four nucleotide bases - adenine (A), guanine (G), cytosine (C), and thymine (T) - in a DNA molecule or fragment. This information is used in various applications such as identifying gene mutations, studying evolutionary relationships, developing molecular markers for breeding, and diagnosing genetic diseases.

The process of DNA Sequence Analysis typically involves several steps, including DNA extraction, PCR amplification (if necessary), purification, sequencing reaction, and electrophoresis. The resulting data is then analyzed using specialized software to determine the exact sequence of nucleotides.

In recent years, high-throughput DNA sequencing technologies have revolutionized the field of genomics, enabling the rapid and cost-effective sequencing of entire genomes. This has led to an explosion of genomic data and new insights into the genetic basis of many diseases and traits.

Viral DNA refers to the genetic material present in viruses that consist of DNA as their core component. Deoxyribonucleic acid (DNA) is one of the two types of nucleic acids that are responsible for storing and transmitting genetic information in living organisms. Viruses are infectious agents much smaller than bacteria that can only replicate inside the cells of other organisms, called hosts.

Viral DNA can be double-stranded (dsDNA) or single-stranded (ssDNA), depending on the type of virus. Double-stranded DNA viruses have a genome made up of two complementary strands of DNA, while single-stranded DNA viruses contain only one strand of DNA.

Examples of dsDNA viruses include Adenoviruses, Herpesviruses, and Poxviruses, while ssDNA viruses include Parvoviruses and Circoviruses. Viral DNA plays a crucial role in the replication cycle of the virus, encoding for various proteins necessary for its multiplication and survival within the host cell.

Viral proteins are the proteins that are encoded by the viral genome and are essential for the viral life cycle. These proteins can be structural or non-structural and play various roles in the virus's replication, infection, and assembly process. Structural proteins make up the physical structure of the virus, including the capsid (the protein shell that surrounds the viral genome) and any envelope proteins (that may be present on enveloped viruses). Non-structural proteins are involved in the replication of the viral genome and modulation of the host cell environment to favor viral replication. Overall, a thorough understanding of viral proteins is crucial for developing antiviral therapies and vaccines.

A virion is the complete, infectious form of a virus outside its host cell. It consists of the viral genome (DNA or RNA) enclosed within a protein coat called the capsid, which is often surrounded by a lipid membrane called the envelope. The envelope may contain viral proteins and glycoproteins that aid in attachment to and entry into host cells during infection. The term "virion" emphasizes the infectious nature of the virus particle, as opposed to non-infectious components like individual capsid proteins or naked viral genome.

Phylogeny is the evolutionary history and relationship among biological entities, such as species or genes, based on their shared characteristics. In other words, it refers to the branching pattern of evolution that shows how various organisms have descended from a common ancestor over time. Phylogenetic analysis involves constructing a tree-like diagram called a phylogenetic tree, which depicts the inferred evolutionary relationships among organisms or genes based on molecular sequence data or other types of characters. This information is crucial for understanding the diversity and distribution of life on Earth, as well as for studying the emergence and spread of diseases.

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Viruses in the Inoviridae, Siphoviridae, and Lipothrixviridae families have been identified. Ocean crust forms at mid-ocean ...
Upon its creation in 1984, the genus was assigned to family Siphoviridae. In 1995, ICTV's sixth report once again changed the ... in the family Siphoviridae. Bacteria serve as natural hosts, with transmission achieved through passive diffusion. There are ...
... (D29) is a cluster A mycobacteriophage belonging to the Siphoviridae family of viruses, it was ... Mycobacterium phage T5 was the first of the siphoviruses found with the structure consistent throughout the Siphoviridae ... The morphotype of mycobacterium virus D29 is that of siphoviridae. The family is known to have an icosahedral head, with long, ... Mycobacterium virus D29 is a Caudovirales virus belonging to the Siphoviridae family. These are commonly referred to as T5-Like ...
In cyanophage S-2L (Siphoviridae), diaminopurine is used instead of adenine (host evasion). Diaminopurine base (Z) pairs ... The Z base is quite widespread in both Siphoviridae and Podoviridae, based on the occurrence of the said gene cluster. In ...
... and Siphoviridae, which can be identified due to their long and flexible tails. Another well studied group of phages with many ...
Transmission electron microscopy indicated that the isolated phages belonged to Siphoviridae, Leviviridae, and Podoviridae ...
... is a genus of viruses in the family Siphoviridae, unassigned to a sub-family. Bacteria serve as the natural host, with ... According to ICTV's 2012 report, the genus was accepted under the name Yualikevirus, assigned to family Siphoviridae, order ...
These bacteriophages have been found to belong to the families Myoviridae, Siphoviridae, Podoviridae, or Tectiviridae. The ... Siphoviridae (S), and Myoviridae (M). UK is unknown/unreported. The DNA polymerase of Bacillus phage phi29 is a unique and ...
... family Siphoviridae. NCBI: Corynephages (list) NCBI: Corynebacterium diphtheriae virus/phage (species) NCBI: Corynephage beta ( ...
It is a proposed species of the family Siphoviridae in the order Caudovirales also known as tailed viruses. It converts a ... Bacteriophage T12, proposed member of family Siphoviridae including related speA-carrying bacteriophages, is also a prototypic ...
... s are ancestrally related to Siphoviridae and possess phage-related genes enabling their integration/excision, replication ...
... is a genus of viruses in the family Siphoviridae. Bacteria and archaea serve as the natural host, with transmission achieved ... assigned only to family Siphoviridae. The whole family was moved to the newly created order Caudovirales in 1998, and the genus ...
It has a double-stranded DNA genome and on morphological grounds appears to be a member of the Siphoviridae. The speA gene was ...
v t e (Articles with 'species' microformats, Taxonbars without primary Wikidata taxon IDs, Siphoviridae, All stub articles, ...
... in the family Siphoviridae. Bacteria serve as natural hosts. There are two species in this genus. The following species are ... assigned to family Siphoviridae in order Caudovirales. In 2012, the genus was renamed to Phic3unalikevirus. The genus was later ...
... (synonym N15-like viruses, N15likevirus) is a genus of viruses in the order Caudovirales, in the family Siphoviridae ... assigned to family Siphoviridae in order Caudovirales. In 2012, the genus was renamed to N15likevirus. The genus was later ...
... in the family Siphoviridae. Bacteria serve as natural hosts. There is only one species in this genus: Bacillus virus SPbeta. ... assigned to family Siphoviridae, order Caudovirales. The genus was renamed to Spbetalikevirus in 2012. The genus was renamed ...
... in the family Siphoviridae. Bacteria serve as natural hosts, with transmission achieved through passive diffusion. There are 59 ... assigned only to family Siphoviridae. The whole family was moved to the newly created order Caudovirales in 1998, and the genus ...
Siphoviridae, Mycobacteriophages, All stub articles, Virus stubs). ...
... assigned only to family Siphoviridae. The whole family was moved to order Caudovirales in 1998, and the genus was renamed to T1 ...
... assigned only to family Siphoviridae. The whole family was moved to order Caudovirales in 1998, and the genus was renamed to T5 ...
... (synonyms c2-like viruses, c2-like phages, C2likevirus) is a genus of viruses in the family Siphoviridae, unassigned ... assigned only to family Siphoviridae. The whole family was moved to the newly created order Caudovirales in 1998, and the genus ...
Siphoviridae, All stub articles, Virus stubs). ...
... all from the family Siphoviridae). The feasibility of using lactic acid bacteria (LAB) as functional protein delivery vectors ...
... thus far have had double-stranded DNA genomes and have been classified by their structure and appearance into siphoviridae or ...
Siphoviridae, All stub articles, Virus stubs). ...
Wikispecies has information related to Siphoviridae. Viralzone: Siphoviridae ICTV Complete Genomes of Siphoviridae (Articles ... Siphoviridae is a family of double-stranded DNA viruses in the order Caudovirales. Bacteria and archaea serve as natural hosts ... Viruses in Siphoviridae are non-enveloped, with icosahedral and head-tail geometries (morphotype B1) or a prolate capsid ( ...
Siphoviridae and Podoviridae. Following from that example, we used some of the methods trialed and tested for the creation of a ...
Familia: Siphoviridae Subfamilia: Arquatrovirinae Genera: Camvirus - Likavirus - R4virus. Subfamilia: Bclasvirinae Genera: ...
Siphoviridae. Genus: λ-like viruses. Species: λ Phage. Enterobacteria phage λ (lambda phage) is a temperate bacteriophage that ...
LH01 Myoviridae, LL5 Siphoviridae,. T4D Myoviridae, LL12 Myoviridae). Bacillus subtilis. DE111® 5 mg **. ...
Ultrastructurally, P2S2 and P5U5 appeared likely to belong to the Podoviridae and Siphoviridae families, respectively. The ...
Hreggviðsson, personal communication). Tth15-6 belongs to the long-tailed phage morphotype of the Siphoviridae family in the ...
PreforPro (LH01-Myoviridae, LL5 - Siphoviridae, T4D - Myoviridae, LL12 - Myoviridae) 15 mg. Other Ingredients:. Vegetable ...
Based on virion morphology, vB_EcoM_P5 was classified as a Myoviridae family phage and vB_SauS_P9 as a Siphoviridae family ...
The results demonstrated that Anellovirus predominated the viral community, followed by Siphoviridae and Myoviridae, and that ...
Brüssow H, Desiere F: Comparative phage genomics and the evolution of Siphoviridae : insights from dairy phages. Mol Microbiol ...
... myoviridae and siphoviridae; and archaeal viruses from the rudiviridae, bicaudaviridae and fuselloviridae. Prokaryotic ...
The company was using a blend of phages from the Siphoviridae family of double-stranded DNA viruses, procured from an outside ...
Bacteriófagos , Siphoviridae , Staphylococcus haemolyticus/genética , DNA Viral/genética , Genoma Viral , Análise de Sequência ... Bacteriófagos , Siphoviridae , Infecções Estafilocócicas , Humanos , Staphylococcus aureus/genética , Esgotos , Filogenia , ... The data suggest that S. aureus Podoviridae and Siphoviridae phages originated from staphylococcal Herelleviridae phages, and ... aureus Podoviridae phages were clustered in the staphylococcal Siphoviridae phage clade. Moreover, phages in different ...
Siphoviridae, Myoviridae, and Podoviridae) of the order Caudovirales (Additional file 1: Figure S2B). The relative abundance of ... Siphoviridae, Myoviridae, and Podoviridae) compared to wastewater type (Additional file 5: Table S4). ... with the majority belonging to the family Siphoviridae (Additional file 1: Figure S3). As previously noted [28], the ...
Complete Genome Sequences of Five SO-1-Like Siphoviridae Bacteriophages That Infect Enterobacteriales. Purnell, Madelyn G; ...
Siphoviridae. Number of Genes. 94. Has been Phamerated?. Yes. Gene List. Click to View. Jasper_1. (helicase) ...
Siphoviridae. Genus: Hk578virus. SPECIES. Escherichia virus HK578. Escherichia virus EK99P1. Escherichia virus JL1. Escherichia ...
Siphoviridae. Non-enveloped, long non-contractile tail. Linear dsDNA Podoviridae. Non-enveloped, short noncontractile tail. ...
OFlaherty, S., R. P. Ross, J. Flynn, W. J. Meaney, G. F. Fitzgerald, and A. Coffey. 2005. Isolation and characterization of two anti-staphylococcal bacteriophages specific for pathogenic Staphylococcus aureus associated with bovine infections. Lett. Appl. Microbiol. 41:482-6. ...
Siphoviridae. A family of BACTERIOPHAGES and ARCHAEAL VIRUSES which are characterized by long, non-contractile tails.. ... Lactococcus lactisLactococcusBacteriophagesSiphoviridaeLeuconostocLactobacillusKluyveromycesStreptococcus thermophilus ... BacterialSiphoviridaeGene Expression Regulation, BacterialLeuconostocLactobacillusBase SequenceDNA, BacterialFermentation ...
Siphoviridae. Has been Phamerated?. No. Publication Info. Uploaded to GenBank?. No. GenBank Accession. None yet. ...
Koga, T., S. Toyoshima, and T. Kawata. 1982. Morphological varieties and host ranges of Vibrio parahaemolyticus bacteriophages isolated from seawater. Appl. Environ. Microbiol. 44:466-470. ...
siphoviridae Path click here NCBI TaxId 186532 NCBI Rank genus NCBI Division phages ...
Urinary Tract Support offers three clinically-studied ingredients that have provide support for the urinary tract or immune system. Pacran® Pacran is a paten…
Dive into the research topics where Sandra Adams is active. These topic labels come from the works of this person. Together they form a unique fingerprint ...
Thus, we propose a new viral genus Oceanospirivirus within the Siphoviridae family to reconcile this cluster, with vB_OliS_GJ44 ... it is proposed that this virus could be classified as the type phage of a new Oceanospirivirus genus within the Siphoviridae ... Thus, we propose a new viral genus Oceanospirivirus within the Siphoviridae family to reconcile this cluster, with vB_OliS_GJ44 ... it is proposed that this virus could be classified as the type phage of a new Oceanospirivirus genus within the Siphoviridae ...
Bacteriophage A falls under the family Siphoviridae of the Group I (dsDNA viruses). Phage lambda is a virus of E. coli K12 ...
Protein fig,387910.2.peg.25: Siphoviridae Staphylococcus aureus phage phiNM4. This feature in The SEED Viewer. NCBI Taxonomy Id ...

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