Rhabdoviridae: A family of bullet-shaped viruses of the order MONONEGAVIRALES, infecting vertebrates, arthropods, protozoa, and plants. Genera include VESICULOVIRUS; LYSSAVIRUS; EPHEMEROVIRUS; NOVIRHABDOVIRUS; Cytorhabdovirus; and Nucleorhabdovirus.Rhabdoviridae Infections: Virus diseases caused by RHABDOVIRIDAE. Important infections include RABIES; EPHEMERAL FEVER; and vesicular stomatitis.Lyssavirus: A genus of the family RHABDOVIRIDAE that includes RABIES VIRUS and other rabies-like viruses.Mononegavirales: An order comprising four families of eukaryotic viruses possessing linear, non-segmented, negative-strand RNA genomes. The families are BORNAVIRIDAE; FILOVIRIDAE; PARAMYXOVIRIDAE; and RHABDOVIRIDAE.Paramyxoviridae: A family of spherical viruses, of the order MONONEGAVIRALES, somewhat larger than the orthomyxoviruses, and containing single-stranded RNA. Subfamilies include PARAMYXOVIRINAE and PNEUMOVIRINAE.Ephrin-A1: An ephrin that was originally identified as the product of an early response gene induced by TUMOR NECROSIS FACTORS. It is linked to the CELL MEMBRANE via a GLYCOINOSITOL PHOSPHOLIPID MEMBRANE ANCHOR and binds EPHA2 RECEPTOR with high affinity. During embryogenesis high levels of ephrin-A1 are expressed in LUNG; KIDNEY; SALIVARY GLANDS; and INTESTINE.Vesiculovirus: A genus of the family RHABDOVIRIDAE that infects a wide range of vertebrates and invertebrates. The type species is VESICULAR STOMATITIS INDIANA VIRUS.Rabies virus: The type species of LYSSAVIRUS causing rabies in humans and other animals. Transmission is mostly by animal bites through saliva. The virus is neurotropic multiplying in neurons and myotubes of vertebrates.Genome, Viral: The complete genetic complement contained in a DNA or RNA molecule in a virus.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Viral Proteins: Proteins found in any species of virus.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.RNA, Viral: Ribonucleic acid that makes up the genetic material of viruses.

*  Rhabdoviridae infections | definition of Rhabdoviridae infections by Medical dictionary
What is Rhabdoviridae infections? Meaning of Rhabdoviridae infections medical term. What does Rhabdoviridae infections mean? ... Looking for online definition of Rhabdoviridae infections in the Medical Dictionary? Rhabdoviridae infections explanation free ... Rhabdoviridae. (redirected from Rhabdoviridae infections). Also found in: Dictionary, Thesaurus, Encyclopedia. Rhab·do·vir·i· ... Rhabdoviridae infections , definition of Rhabdoviridae infections by Medical dictionary https://medical-dictionary. ...
  http://medical-dictionary.thefreedictionary.com/Rhabdoviridae+infections
*  Immunogenicity and Safety of Rabies Vaccine, Administered With Two Different Simulated Post Exposure Schedules - Full Text View...
Rhabdoviridae Infections. Mononegavirales Infections. RNA Virus Infections. Virus Diseases. Vaccines. Immunologic Factors. ...
  https://clinicaltrials.gov/ct2/show/NCT01365494
*  Safety and Tolerability of CV8102 Alone and in Combination With a Rabies Virus Vaccine in Healthy Adults - Full Text View -...
Intending to travel to regions/countries for which rabies vaccinations are recommended or where high risk of infection exists ... urinary tract infections, inflammatory, allergic or traumatic conditions that may interfere with safety assessment of the ...
  https://clinicaltrials.gov/ct2/show?term=curevac&rank=3
*  Hart Park virus - Wikipedia
"Rhabdoviridae". Retrieved 19 October 2013. "Rhabdoviridae Infections." The Free Dictionary. Farlex, n.d. Web. 19 Oct. 2013.. ... The host transition from birds to humans plays a huge role in the potential for widespread viral infections of the HP virus and ... The HP virus is classified in the Rhabdoviridae family and is related to the viral agents that cause rabies and vesicular ... The Hart Park virus does not currently have a disease related to its infection in other animals, but its transmission through ...
  https://en.wikipedia.org/wiki/Hart_Park_virus
*  Viruses | Free Full-Text | Plant Translation Factors and Virus Resistance
... that specifically interact with viral RNAs and proteins and regulate various aspects of the infection cycle. Emerging evidence ... Resistance to Rhabdoviridae Infection and Subversion of Antiviral Responses. Previous Article in Journal. NLRP3 Inflammasome ... that specifically interact with viral RNAs and proteins and regulate various aspects of the infection cycle. Emerging evidence ...
  http://www.mdpi.com/1999-4915/7/7/3392
*  Transcription levels of immune genes characteristic of | Open-i
At days 1, 3 and 5 post-infection six trout from each group were killed and the liver sampled to determine the levels of ... At days 1, 3 and 5 post-infection six trout from each group were killed and the liver sampled to determine the levels of ... Consequently, flow cytometry analysis of CD8α+ cells in liver and spleen at day 5 post-infection revealed a decrease in the ... Consequently, flow cytometry analysis of CD8α+ cells in liver and spleen at day 5 post-infection revealed a decrease in the ...
  https://openi.nlm.nih.gov/detailedresult.php?img=PMC4206492_pone.0111084.g003&req=4
*  Peptides and Proteins: Novus Biologicals
Rhabdoviridae Infections (2). *. Rheumatism (2). *. Rheumatoid Arthritis (4). *. Riboflavin Deficiency (8). *. Russell-silver ...
  https://www.novusbio.com/product-type/peptides-and-proteins?related_diseases=Multiple%20Acyl%20Coenzyme%20A%20Dehydrogenase%20Deficiency
*  2017/18 ICD-10-CM Diagnosis Code A87.9: Viral meningitis, unspecified
... rhabdoviridae infections; arenaviridae infections; herpesviridae infections; adenoviridae infections; jc virus infections; and ... Togaviridae infections; flaviviridae infections; rubella; bunyaviridae infections; orbivirus infections; picornaviridae ... Viral infections of the leptomeninges and subarachnoid space.. *Viral infections of the leptomeninges and subarachnoid space. ... Other viral infections of central nervous system, not elsewhere classified. 2016 2017 2018 Non-Billable/Non-Specific Code Type ...
  http://www.icd10data.com/ICD10CM/Codes/A00-B99/A80-A89/A87-/A87.9
*  ASMscience | Advances in Myeloid-Like Cell Origins and Functions in the Model Organism |span class='jp-italic'|Drosophila...
The transcriptional response of Drosophila melanogaster to infection with the sigma virus (Rhabdoviridae). PLoS One 4:e6838. ... RNAi screen in Drosophila cells reveals the involvement of the Tom complex in Chlamydia infection. PLoS Pathog 3:1446-1458. [ ... Role for lysosomal enzyme β-hexosaminidase in the control of mycobacteria infection. Proc Natl Acad Sci U S A 105:710-715. [ ... The Toll-dorsal pathway is required for resistance to viral oral infection in Drosophila. PLoS Pathog 10:e1004507. doi:10.1371/ ...
  http://www.asmscience.org/content/journal/microbiolspec/10.1128/microbiolspec.MCHD-0038-2016
*  Peregrinus maidis - Wikipedia
Rhabdoviridae: Nucleorhabdovirus) infection". Environmental Entomology. 42: 949-956. doi:10.1603/en12321. Brewbaker, J. L. ( ... In addition to physical damage, P. maidis can also introduce mold and infection to the plant while piercing the tissues. P. ... The genetically modified corn had decreased infection and transmission of MMV, but did not affect the fitness of P. maidis or ... Therefore, usually only adults are able to pass the infection onto a healthy plant. Macropterous adults dispersing to a new ...
  https://en.wikipedia.org/wiki/Peregrinus_maidis
*  List of MeSH codes (C02) - Wikipedia
... rubulavirus infections MeSH C02.782.580.600.680.500 --- mumps MeSH C02.782.580.830 --- rhabdoviridae infections MeSH C02.782. ... deltaretrovirus infections MeSH C02.782.815.200.260 --- enzootic bovine leukosis MeSH C02.782.815.200.470 --- htlv-i infections ... cardiovirus infections MeSH C02.782.687.207 --- common cold MeSH C02.782.687.359 --- enterovirus infections MeSH C02.782. ... avulavirus infections MeSH C02.782.580.600.080.600 --- newcastle disease MeSH C02.782.580.600.400 --- henipavirus infections ...
  https://en.wikipedia.org/wiki/List_of_MeSH_codes_(C02)
*  Animal Bites and Rabies - Health Encyclopedia - University of Rochester Medical Center
Rabies is a viral infection of certain warm-blooded animals and is caused by a virus in the Rhabdoviridae family. It attacks ... Once the infection is established in the brain, the virus travels down the nerves from the brain and multiplies in different ... Watch for signs of infection at the site, such as increased redness or pain, swelling, drainage, or if the person develops a ... Cat scratches, even from a kitten, can carry "cat scratch disease," a bacterial infection. Other animals can transmit rabies ...
  https://www.urmc.rochester.edu/encyclopedia/content.aspx?ContentTypeID=85&ContentID=P00819
*  Welcome to CDC stacks | Antibodies against Lagos Bat Virus in Megachiroptera from West Africa - 17100 | Emerging Infectious...
Rhabdoviridae Infections Dispatch * Description: To investigate the presence of Lagos bat virus (LBV)-specific antibodies in ... This occurred approximately 80 km from the site where the only other known human infection with the virus had occurred 36 year ...
  https://stacks.cdc.gov/view/cdc/17100
*  Welcome to CDC stacks | Novel Lyssaviruses Isolated from Bats in Russia - 14360 | Emerging Infectious Diseases
Rhabdoviridae Infections Rhabdovirus RNA, Viral Russia Sequence Analysis, DNA Virus * Description: Two new rabies-related ...
  https://stacks.cdc.gov/view/cdc/14360
*  Infectious Disease | Health Information | MedCentral Health System
Rabies is a viral infection of certain warm-blooded animals and is caused by a virus in the Rhabdoviridae family. It attacks ... Meningococcal Infections Meningococcal Infections What are meningococcal infections? Meningococcal infections are caused by a ... Other infections caused by HPIV include infections of the lower respiratory tract such as croup (an infection of the airway ... infection of the membranes that surround the brain and spinal cord) and blood stream infections. Meningococcal infections are ...
  http://www.medcentral.org/Main/StaywellProducts.aspx?taxonomy=InfectiousDiseaseSw2
*  NewYork-Presbyterian/Queens - Rabies
Rabies is a viral infection of certain warm-blooded animals and is caused by a virus in the Rhabdoviridae family. It attacks ... Once the infection reaches the brain, the virus travels into the nerves and multiplies in different organs. ...
  http://www.nyhq.org/diw/content.asp?PageID=P02845&More=DIW
*  Pets and Infectious Diseases - Health Encyclopedia - University of Rochester Medical Center
Rabies is a widespread, viral infection of warm-blooded animals. Caused by a virus in the Rhabdoviridae family, it attacks the ... Once the infection is established in the brain, the virus travels down the nerves from the brain and multiplies in different ... Cat scratches, for example, even from a kitten, may carry "cat scratch disease," a bacterial infection. Bites and/or scratches ...
  https://www.urmc.rochester.edu/encyclopedia/content.aspx?ContentTypeID=85&ContentID=P00641&redir=urmc.rochester.edu
*  Massively parallel pyrosequencing-based transcriptome analyses of small brown planthopper (Laodelphax striatellus), a vector...
The transcriptional response of Drosophila melanogaster to infection with the sigma virus (Rhabdoviridae). PLoS One. 2009, 4 (8 ... At present we are using more precise methods to investigate the functional roles of these candidate genes during RSV infection ... Teixeira L, Ferreira A, Ashburner M: The bacterial symbiont Wolbachia induces resistance to RNA viral infections in Drosophila ... Drakesmith H, Prentice A: Viral infection and iron metabolism. Nat Rev Microbiol. 2008, 6 (7): 541-552. 10.1038/nrmicro1930. ...
  https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-11-303
*  Protocols and Video Articles Authored by Timothy Block
... a prototype member of the Rhabdoviridae family. Taking advantage of this well-studied virus infection system, we show that ... Here it is shown that geneticin prevented the cytopathic effect (CPE) resulting from DENV-2 infection of BHK cells, in a dose- ... Feb, 2008 , Pubmed ID: 18077728 Hepatitis C virus (HCV) infection is a common cause of chronic hepatitis and is currently ... The results revealed that (i) both types of IFNs could efficiently prevent the WNV infection, but IFN-alpha demonstrated a ...
  https://www.jove.com/author/Timothy_Block
*  NewYork-Presbyterian Queens - Glossary - Non-Traumatic Emergencies
rabies - a widespread, viral infection of warm-blooded animals; caused by a virus in the Rhabdoviridae family, it attacks the ... often the cause of urinary tract infections, diarrhea in infants, and wound infections. ... influenza (Also called the flu.) - a viral respiratory tract infection. The influenza viruses are divided into three types: A, ... Lyme disease (LD) - a multi-stage, multi-system bacterial infection caused by the spirochete Borrelia burgdorferi, a spiral- ...
  http://www.nyhq.org/diw/Content.asp?PageID=DIW000842&More=OTH&language=Korean
*  NewYork-Presbyterian Queens - Rabies
Rabies is a widespread, viral infection of warm-blooded animals. Caused by a virus in the Rhabdoviridae family, it attacks the ... Once the infection is established in the brain, the virus travels down the nerves from the brain and multiplies in different ... Cat scratches, for examples, even from a kitten can carry "cat scratch disease," a bacterial infection. Other animals can ...
  http://www.nyhq.org/diw/Content.asp?PageID=DIW001450&More=DIW
*  Academic Book: Classroom Lectures on Human Viral Diseases Presented at the City University of New York. An Explanation of...
Family Rhabdoviridae. 1. Rabies. IV. Family Bunyaviridae. 1. California and La Crosse Encephalitides. 2. Hantavirus infection. ... 2. Human Papillomavirus (HPV) Infections. 2.1 Venereal Warts Infection. 2.2 Mouth Cancer. III. Family Adenoviridae. Lecture IV ... 1. Herpes Simplex Virus 1 (HSV-1) Infection. 2. Herpes Simplex Virus 2 (HSV-2) Infection. Genus Varicellovirus. 3. Chickenpox ... 2. Coxsackievirus Infections. 3. Hepatitis A II. Family Flaviridae. 1. Hepatitis C. 2. Hepatitis G. 3. Yellow Fever. 4. Dengue ...
  http://mellenpress.com/book/Classroom-Lectures-on-Human-Viral-Diseases-Presented-at-the-City-University-of-New-York-An-Explanation-of-Basic-Principles/8859/
*  Publications - SECTION OF EVOLUTIONARY MEDICINE
"The transcriptional response of Drosophila melanogaster to infection with the sigma virus (Rhabdoviridae)", PloS ONE, Vol. 4 No ... Expression of the Blood-Group-Related Gene B4galnt2 Alters Susceptibility to Salmonella Infection. PLoS Pathogens, Vol. 11 No. ...
  http://web.evolbio.mpg.de/evolgenomics/publications.html
*  Bacteriophage therapy to combat bacterial infections in poultry | Virology Journal | Full Text
Infections in poultry are an economic and health problem in Europe and worldwide. The most common infections are associated ... including chronic infection, pseudolysogeny and abortive infection (Fig. 1). Not all of these cycles end with the death of the ... resulting in clinical or subclinical infections. In the US, annual economic losses due to Salmonella infections run from $1.188 ... It should be emphasized that the percentage of birds resistant to a second Campylobacter infection was very low, at about 2% [ ...
  https://virologyj.biomedcentral.com/articles/10.1186/s12985-017-0849-7
*  Rabies | Boston Children's Hospital
Rabies is a viral infection of certain warm-blooded animals (such as skunks, raccoons, foxes, coyotes and bats) and is caused ... by a virus in the Rhabdoviridae family. It attacks the nervous system and, once symptoms develop, it is 100 percent fatal in ...
  http://www.childrenshospital.org/conditions-and-treatments/conditions/r/rabies

Sigma viruses: Sigma viruses are a clade of viruses in the family Rhabdoviridae that naturally infect dipterans, and have recently been proposed to represent a new genus of rhabdoviruses.Longdon B and Walker PJ (2011) Sigma virus genus proposal for the International Committee on Taxonomy of Viruses.Australian bat lyssavirus: Australian bat lyssavirus (ABLV) (initially named pteropid lyssavirus PLV) is a zoonotic virus closely related to rabies virus. It was first identified in a 5-month-old juvenile Black Flying Fox (Pteropus alecto) collected near Ballina in northern New South Wales, Australia in January 1995 during a national surveillance program for the recently identified Hendra virus.Mononegavirales: The order Mononegavirales is the taxonomic home of numerous related viruses. Members of the order that are commonly known are, for instance, Ebola virus, human respiratory syncytial virus, measles virus, mumps virus, Nipah virus, and rabies virus.Vesiculovirus matrix proteins: The family of vesiculovirus matrix proteins consists of several matrix proteins of the vesicular stomatitis virus, also known as VSIV or VSV. The matrix (M) protein of the virus causes many of the cytopathic effects of VSV, including an inhibition of host gene expression and the induction of cell rounding.Rabies virus: The rabies virus is a neurotropic virus that causes rabies in humans and animals. Rabies transmission can occur through the saliva of animals and less commonly through contact with human saliva.Branching order of bacterial phyla (Gupta, 2001): There are several models of the Branching order of bacterial phyla, one of these was proposed in 2001 by Gupta based on conserved indels or protein, termed "protein signatures", an alternative approach to molecular phylogeny. Some problematic exceptions and conflicts are present to these conserved indels, however, they are in agreement with several groupings of classes and phyla.Coles PhillipsDNA sequencer: A DNA sequencer is a scientific instrument used to automate the DNA sequencing process. Given a sample of DNA, a DNA sequencer is used to determine the order of the four bases: G (guanine), C (cytosine), A (adenine) and T (thymine).

(1/370) Gene transfer to human pancreatic endocrine cells using viral vectors.

We have studied the factors that influence the efficiency of infection of human fetal and adult pancreatic endocrine cells with adenovirus, murine retrovirus, and lentivirus vectors all expressing the green fluorescent protein (Ad-GFP, MLV-GFP, and Lenti-GFP, respectively). Adenoviral but not retroviral vectors efficiently infected intact pancreatic islets and fetal islet-like cell clusters (ICCs) in suspension. When islets and ICCs were plated in monolayer culture, infection efficiency with all three viral vectors increased. Ad-GFP infected 90-95% of the cells, whereas infection with MLV-GFP and Lenti-GFP increased only slightly. Both exposure to hepatocyte growth factor/scatter factor (HGF/SF) and dispersion of the cells by removal from the culture dish and replating had substantial positive effects on the efficiency of infection with retroviral vectors. Studies of virus entry and cell replication revealed that cell dispersion and stimulation by HGF/SF may be acting through both mechanisms to increase the efficiency of retrovirus-mediated gene transfer. Although HGF/SF and cell dispersion increased the efficiency of infection with MLV-GFP, only rare cells with weak staining for insulin were infected, whereas approximately 25% of beta-cells were infected with Lenti-GFP. We conclude that adenovirus is the most potent vector for ex vivo overexpression of foreign genes in adult endocrine pancreatic cells and is the best vector for applications where high-level but transient expression is desired. Under the optimal conditions of cell dispersion plus HGF/SF, infection with MLV and lentiviral vectors is reasonably efficient and stable, but only lentiviral vectors efficiently infect pancreatic beta-cells.  (+info)

(2/370) A novel approach to visualize polyclonal virus-specific CD8 T cells in vivo.

Recent technical breakthroughs in generating soluble MHC class I-peptide tetramers now allow the direct visualization of virus-specific CD8 T cells after infection in vivo. However, this technique requires the knowledge of the immunodominant viral epitopes recognized by T cells. Here, we describe an alternative approach to visualize polyclonal virus-specific CD8 T cells in vivo using a simple adoptive transfer system. In our approach, C57BL/6 (Thy1.2) mice were infected with lymphocytic choriomeningitis virus, vesicular stomatitis virus, or vaccinia virus to induce virus-specific memory T cells. Tracer T cells (2 x 106) from these virus-immune mice were adoptively transferred into nonirradiated (C57BL/6 x B6.PL-Thy-1a)F1 mice. After infection of the F1-recipient mice with the appropriate virus, the transferred cells expanded vigorously, and on day 8 postinfection 60-80% of total CD8 T cells were of donor T cell origin. Under the same conditions memory CD4 T cells gave rise to at least 10 times less cell numbers than memory CD8 T cells. The transfer system described here not only allows to visualize effector and memory CD8 T cells in vivo but also to isolate them for further in vitro characterization without knowing the epitopes recognized by these Ag-specific CD8 T cells.  (+info)

(3/370) Identification of the murine Mx2 gene: interferon-induced expression of the Mx2 protein from the feral mouse gene confers resistance to vesicular stomatitis virus.

The mouse genome contains two related interferon-regulated genes, Mx1 and Mx2. Whereas Mx1 codes for the nuclear 72-kDa protein that interferes with influenza virus replication after interferon treatment, the Mx2 gene is nonfunctional in all laboratory mouse strains examined, since its open reading frame (ORF) is interrupted by an insertional mutation and a subsequent frameshift mutation. In the present study, we demonstrate that Mx2 mRNA of cells from feral mouse strains NJL (Mus musculus musculus) and SPR (Mus spretus) differs from that of the laboratory mouse strains tested. The Mx2 mRNA of the feral strains contains a single long ORF consisting of 656 amino acids. We further show that Mx2 protein in the feral strains is expressed upon interferon treatment and localizes to the cytoplasm much like the rat Mx2 protein, which inhibits vesicular stomatitis virus replication. Furthermore, transfected 3T3 cell lines of laboratory mouse origin expressing Mx2 from feral strains acquire slight resistance to vesicular stomatitis virus.  (+info)

(4/370) Australian bat lyssavirus infection in a captive juvenile black flying fox.

The newly emerging Australian bat lyssavirus causes rabieslike disease in bats and humans. A captive juvenile black flying fox exhibited progressive neurologic signs, including sudden aggression, vocalization, dysphagia, and paresis over 9 days and then died. At necropsy, lyssavirus infection was diagnosed by fluorescent antibody test, immunoperoxidase staining, polymerase chain reaction, and virus isolation. Eight human contacts received postexposure vaccination.  (+info)

(5/370) Temperature dependent characteristics of a recombinant infectious hematopoietic necrosis virus glycoprotein produced in insect cells.

A recombinant infectious hematopoietic necrosis virus (IHNV) glycoprotein (G protein) was produced in insect cells using a baculovirus vector (Autographa californica nuclear polyhedrosis virus). Characteristics of this protein were evaluated in relation to native viral G protein. A full-length (1.6 kb) cDNA copy of the glycoprotein gene of IHNV was inserted into the baculovirus vector under control of the polyhedrin promoter. High levels of G protein (approximately 0.5 microgram/1 x 10(5) cells) were produced in Spodoptera frugiperda (Sf9) cells following recombinant baculovirus infection. Analysis of cell lysates by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot revealed a recombinant IHNV G of slightly higher mobility on the gel than the viral G protein. Differences in mobility were abrogated by endoglycosidase treatment. When the recombinant G protein was produced in insect cells at 20 degrees C (RecGlow), immunostaining and cell fusion activity demonstrated surface localization of the protein. In contrast, when recombinant protein was produced at 27 degrees C (RecGhigh), G protein was sequestered within the cell, suggesting that at the 2 different temperatures processing differences may exist. Eleven monoclonal antibodies (MAbs) were tested by immunoblotting for reactivity to the recombinant G protein. All 11 MAbs reacted to the reduced proteins. Four MAbs recognized both RecGhigh and RecGlow under non-reducing conditions; however, 1 neutralizing MAb (92A) recognized RecGlow but failed to react to RecGhigh under non-reducing conditions. This suggests that differences exist between RecGlow and RecGhigh which may have implications in the development of a properly folded recombinant G protein with the ability to elicit protective immunity in fish.  (+info)

(6/370) Immunogenicity of a recombinant infectious hematopoietic necrosis virus glycoprotein produced in insect cells.

A recombinant infectious hematopoietic necrosis virus (IHNV) glycoprotein (G protein), produced in Spodoptera frugiperda (Sf9) cells following infection with a baculovirus vector containing the full-length (1.6 kb) glycoprotein gene, provided very limited protection in rainbow trout Oncorhynchus mykiss challenged with IHNV. Fish were injected intraperitoneally (i.p.) with Sf9 cells grown at 20 degrees C (RecGlow) or 27 degrees C (RecGhigh) expressing the glycoprotein gene. Various antigen (Ag) preparations were administered to adult rainbow trout or rainbow trout fry. Sera collected from adult fish were evaluated for IHNV neutralization activity by a complement-dependent neutralization assay. Anti-IHNV neutralizing activity was observed in sera, but the percent of fish responding was significantly lower (p < 0.05) in comparison to fish immunized with a low virulence strain of IHNV (LV-IHNV). A small number of fish immunized with RecGlow or RecGhigh possessed IHNV G protein specific antibodies (Abs) in their serum. Cumulative mortality (CM) of rainbow trout fry (mean weight, 1 g) vaccinated by i.p. injection of freeze/thawed Sf9 cells producing RecGlow was 18% in initial trials following IHNV challenge. This level of protection was significant (p < 0.05) but was not long lasting, and neutralizing Abs were not detected in pooled serum samples. When trout fry (mean weight, 0.6 g) were vaccinated with supernatant collected from sonicated Sf9 cells, Sf9 cells producing RecGlow, or Sf9 cells producing RecGhigh, CM averaged 46%. Protection was enhanced over negative controls, but not the positive controls (2% CM), suggesting that in the first trial soluble cellular proteins may have provided some level of non-specific protection, regardless of recombinant protein expression. Although some immunity was elicited in fish, and RecGlow provided short-term protection from IHNV, Ab-mediated protection could not be demonstrated. The results suggest that recombinant G proteins produced in insect cells lack the immunogenicity associated with vaccination of fish with an attenuated strain of IHNV.  (+info)

(7/370) Mutations in the glycoprotein of viral haemorrhagic septicaemia virus that affect virulence for fish and the pH threshold for membrane fusion.

To study the molecular basis of virulence of viral haemorrhagic septicaemia virus (VHSV), we used a cross-reactive neutralizing MAb to select MAb-resistant (MAR) mutants with reduced pathogenicity for fish. From sequence determination of the G gene of MAR mutants, attenuated laboratory variant and avirulent field strains, we identified two distant regions of the glycoprotein associated with virulence: region I (aa 135-161), homologous to the putative fusion peptide of both rabies virus (RV) and vesicular stomatitis virus (VSV), and region II (surrounding aa 431-433), homologous to RV and VSV domains controlling the conformational changes necessary for the fusion process to take place. Simultaneous mutations in both regions resulted in the most attenuated phenotype and we obtained genetic evidence that regions I and II may be structurally linked. As the MAR mutants had mutations in or near domains involved in fusion, the fusion properties of VHSV and its variants were analysed. This work allowed us to postulate that the fusion domain of VHSV is probably constituted of two distinct regions of the protein connected through a disulfide bridge between cysteines 110 and 152. Finally, we obtained evidence suggesting that the pH threshold for fusion is a determinant for virulence: restriction of fusion to a more acidic pH was associated with attenuation for the variant tr25 which had a shift of the threshold for maximal fusion from pH 6.30 (for the parental strain) to pH 6.00; conversely, two field strains which had maximal fusion at pH 6.60 were the most virulent.  (+info)

(8/370) Neuronal expression of NOS-1 is required for host recovery from viral encephalitis.

The role of nitric oxide synthase (NOS) in host defense and clearance of vesicular stomatitis virus (VSV) from the central nervous system (CNS) was examined. NOS-1, NOS-2, and NOS-3 knockout mice were infected with VSV and were treated with either IL-12 or medium. IL-12 treatment resulted in substantially decreased VSV titers in wildtype and NOS-3 knockout mice, but had a marginal effect in the NOS-1 and NOS-2 knockout mice. NOS-1 expression in neurons was associated with survival from VSV infection. The data indicate that the enzyme activity is local, since NOS-2 expression in microglia and inflammatory macrophages and NOS-3 expression in astrocytes, endothelial cells, and ependymal cells did not compensate.  (+info)



  • family
  • In addition, pairwise identities of the deduced amino acid sequence of the L gene of RFFRV with that of other rhabdoviruses of the Rhabdoviridae family were only [less than or equal to] 35% (online Technical Appendix Table 2). (freethesaurus.com)
  • With this information, CDC scientists explored further and classified it as a member of Rhabdoviridae family and finally identified it as Chandipura virus (Pallansch M, personal communication). (freethesaurus.com)