Reproductive Techniques, Assisted: Clinical and laboratory techniques used to enhance fertility in humans and animals.Reproductive Techniques: Methods pertaining to the generation of new individuals, including techniques used in selective BREEDING, cloning (CLONING, ORGANISM), and assisted reproduction (REPRODUCTIVE TECHNIQUES, ASSISTED).Sperm Injections, Intracytoplasmic: An assisted fertilization technique consisting of the microinjection of a single viable sperm into an extracted ovum. It is used principally to overcome low sperm count, low sperm motility, inability of sperm to penetrate the egg, or other conditions related to male infertility (INFERTILITY, MALE).Triplets: Three individuals derived from three FETUSES that were fertilized at or about the same time, developed in the UTERUS simultaneously, and born to the same mother.Infertility, Male: The inability of the male to effect FERTILIZATION of an OVUM after a specified period of unprotected intercourse. Male sterility is permanent infertility.Fertilization in Vitro: An assisted reproductive technique that includes the direct handling and manipulation of oocytes and sperm to achieve fertilization in vitro.Insemination, Artificial: Artificial introduction of SEMEN or SPERMATOZOA into the VAGINA to facilitate FERTILIZATION.Pregnancy: The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.Pregnancy, Multiple: The condition of carrying two or more FETUSES simultaneously.Pregnancy Rate: The ratio of the number of conceptions (CONCEPTION) including LIVE BIRTH; STILLBIRTH; and fetal losses, to the mean number of females of reproductive age in a population during a set time period.Embryo Transfer: The transfer of mammalian embryos from an in vivo or in vitro environment to a suitable host to improve pregnancy or gestational outcome in human or animal. In human fertility treatment programs, preimplantation embryos ranging from the 4-cell stage to the blastocyst stage are transferred to the uterine cavity between 3-5 days after FERTILIZATION IN VITRO.Azoospermia: A condition of having no sperm present in the ejaculate (SEMEN).Pregnancy Outcome: Results of conception and ensuing pregnancy, including LIVE BIRTH; STILLBIRTH; SPONTANEOUS ABORTION; INDUCED ABORTION. The outcome may follow natural or artificial insemination or any of the various ASSISTED REPRODUCTIVE TECHNIQUES, such as EMBRYO TRANSFER or FERTILIZATION IN VITRO.Ovulation Induction: Techniques for the artifical induction of ovulation, the rupture of the follicle and release of the ovum.Pregnancy Reduction, Multifetal: Selective abortion of one or more embryos or fetuses in a multiple gestation pregnancy. The usual goal is to improve the outcome for the remaining embryos or fetuses.Spermatozoa: Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility.Twins: Two individuals derived from two FETUSES that were fertilized at or about the same time, developed in the UTERUS simultaneously, and born to the same mother. Twins are either monozygotic (TWINS, MONOZYGOTIC) or dizygotic (TWINS, DIZYGOTIC).Semen Analysis: The quality of SEMEN, an indicator of male fertility, can be determined by semen volume, pH, sperm concentration (SPERM COUNT), total sperm number, sperm viability, sperm vigor (SPERM MOTILITY), normal sperm morphology, ACROSOME integrity, and the concentration of WHITE BLOOD CELLS.Ovarian Hyperstimulation Syndrome: A complication of OVULATION INDUCTION in infertility treatment. It is graded by the severity of symptoms which include OVARY enlargement, multiple OVARIAN FOLLICLES; OVARIAN CYSTS; ASCITES; and generalized EDEMA. The full-blown syndrome may lead to RENAL FAILURE, respiratory distress, and even DEATH. Increased capillary permeability is caused by the vasoactive substances, such as VASCULAR ENDOTHELIAL GROWTH FACTORS, secreted by the overly-stimulated OVARIES.Infertility: Inability to reproduce after a specified period of unprotected intercourse. Reproductive sterility is permanent infertility.Cryopreservation: Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.Sperm Motility: Movement characteristics of SPERMATOZOA in a fresh specimen. It is measured as the percentage of sperms that are moving, and as the percentage of sperms with productive flagellar motion such as rapid, linear, and forward progression.Semen: The thick, yellowish-white, viscid fluid secretion of male reproductive organs discharged upon ejaculation. In addition to reproductive organ secretions, it contains SPERMATOZOA and their nutrient plasma.Sperm Count: A count of SPERM in the ejaculum, expressed as number per milliliter.Fertilization: The fusion of a spermatozoon (SPERMATOZOA) with an OVUM thus resulting in the formation of a ZYGOTE.Infertility, Female: Diminished or absent ability of a female to achieve conception.Oocytes: Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).

*  Different ART outcomes at increasing peak estradiol levels with long and antagonist protocols: retrospective insights from ten...

... levels on assisted reproductive technologies outcomes in high responders (≥12 oocytes retrieved) according to the controlled ... Reproductive Techniques, Assisted*. Retrospective Studies. Treatment Outcome. Chemical. Reg. No./Substance: 33515-09-2/ ... PURPOSE: To evaluate the impact of high estradiol (E2) levels on assisted reproductive technologies outcomes in high responders ... Title: Journal of assisted reproduction and genetics Volume: 28 ISSN: 1573-7330 ISO Abbreviation: J. Assist. Reprod. Genet. ...
biomedsearch.com/nih/Different-ART-outcomes-at-increasing/21519843.html

*  DMOZ - Health: Reproductive Health: Infertility: Management: Assisted Reproductive Technologies: Embryo Screening

A technique that could revolutionise IVF treatment for older women has produced its first British baby. (August 04, 2003) ... Telegraph: Egg-testing technique 'doubles the chances of older women on IVF' ...
dmoztools.net/Health/Reproductive_Health/Infertility/Management/Assisted_Reproductive_Technologies/Embryo_Screening/

*  ASSISTED REPRODUCTIVE TECHNOLOGY Adopted PDF

ASSISTED REPRODUCTIVE TECHNOLOGY Adopted 2013 The Christian Medical & Dental Association is a public voice for Christian ... Artificial insemination What is involved? Artificial insemination is an assisted reproduction technique that consists of ... Assisted Reproductive Technologies at IGO 9339 Genesee Avenue, Suite 220 San Diego, CA 92121 858 455 7520 Assisted Reproductive ... Ethical issues in assisted reproductive technologies. Effy Vayena Ethical issues in assisted reproductive technologies Effy ...
docplayer.net/9057229-Assisted-reproductive-technology-adopted-2013.html

*  Ethical considerations arising from the use of assisted reproductive technologies.

Oftentimes, the widespread use of reproductive techniques and fertility-e ... The rapid growth of reproductive medicine has in many cases occurred faster than societies have been able to assimilate the ... Oftentimes, the widespread use of reproductive techniques and fertility-enhancing medications has occurred before the ethical ... The rapid growth of reproductive medicine has in many cases occurred faster than societies have been able to assimilate the ...
biomedsearch.com/nih/Ethical-considerations-arising-from-use/22549714.html

*  Textbook of Assisted Reproductive Techniques Fourth Edition 4th edition | 9781841849720 | VitalSource

Buy or Rent Textbook of Assisted Reproductive Techniques Fourth Edition as an eTextbook and get instant access. ... Textbook of Assisted Reproductive Techniques Fourth Edition 4th Edition by David K. Gardner and Publisher CRC Press. Save up to ...
https://vitalsource.com/en-uk/products/textbook-of-assisted-reproductive-techniques-david-k-gardner-v9781841849737

*  Recent Horse Health and Welfare News | The Horse | TheHorse.com

Three Assisted Reproductive Techniques for Subfertile Mares. March 17, 2017. Vets can use intracytoplasmic sperm injection, ...
thehorse.com/news/by-topic/45/assisted-reproduction?page=1

*  Assisted Reproductive Technology | IVF | MedlinePlus

Learn about treating infertility with assisted reproductive technology (ART). It includes procedures like in vitro ... ClinicalTrials.gov: Reproductive Techniques, Assisted (National Institutes of Health) Journal Articles References and abstracts ... Assisted Reproductive Technologies (American Society for Reproductive Medicine) - PDF Also in Spanish ... Assisted Reproductive Technology (ART) (National Institute of Child Health and Human Development) Also in Spanish ...
https://medlineplus.gov/assistedreproductivetechnology.html

*  Mia Moda Now Offers a Rich Collection of Fabrics for Sale | Webnewswire

IVF and Other Assisted Reproductive Technique Babies Are As Intelligent As Naturally Conceived Ones. June 28, 2017 ...
webnewswire.com/2016/11/14/mia-moda-now-offers-a-rich-collection-of-fabrics-for-sale/

*  More4Kids: Parenting Books: The Infertility Cure: The Ancient Chinese Wellness Program for Getting Pregnant and...

Improving the outcome of assisted reproductive techniques. The Infertility Cure opens the door to new ideas about treating ... treating infertility using traditional Chinese medicine-on its own or in conjunction with the latest Western-based reproductive ...
more4kids.info/shop/1000-1000-0316159212-The_Infertility_Cure_The_Ancient_Chinese_Wellness_Program_for_Getting___Pregnant_and_Having_Healthy_Babies.html

*  New IVF guidance - NHSUK

The HFEA will publish new advice on assisted reproductive techniques in April. ... Assisted reproductive technology and major structural birth defects in the United States.Hum Reprod 2009; Advance Access ... particularly interested in the number of birth defects in the offspring of women who had used assisted reproductive techniques ... Notwithstanding its limitations, the study provides an important direction for future research into how assisted reproduction ...
https://nhs.uk/news/pregnancy-and-child/new-ivf-guidance/

*  Medical Modeling & Simulation Database

Experts in Assisted Reproductive Techniques Achieve High Pregnancy Success Rates. June. 29. ...
evms.edu/research/centers_institutes_departments/national_center_for_collaboration_in_medical_model/programs_initiatives/medical_modeling_simulation_database/

*  Varicocele repair for infertility: what is the evidence?

Next Document: Varicocele repair in the era of modern assisted reproductive techniques.. ... The main alternative remains the use of artificial reproductive techniques.. ...
biomedsearch.com/nih/Varicocele-repair-infertility-what-evidence/23026895.html

*  Daniel Cramer

Recent trends in assisted reproductive techniques and associated outcomes. D W Cramer. Department of Obstetrics and Gynecology ... J Assist Reprod Genet 20:210-5. 2003. ..Measurements of TSH and prolactin are generally included in the evaluation of female ... reproductive history*habits*follicular phase*polarization microscopy*boston*puerperal disorders*ca 15 3 antigen*electron probe ... Puerperal mastitis: a reproductive event of importance affecting anti-mucin antibody levels and ovarian cancer risk. Daniel W ...
https://labome.org/expert/usa/massachusetts/cramer/daniel-cramer-464438.html

*  Spreading smiles all the way | education | Hindustan Times

If these treatments don't work, assisted -reproductive techniques are opted for. They include IUI (intra uterine insemination ...
hindustantimes.com/education/spreading-smiles-all-the-way/story-mgKRu7wGmdSCPDwbNOTg2O.html

*  Abnormal Cervical Mucus - Gynecology and Obstetrics - Merck Manuals Professional Edition

Assisted Reproductive Techniques. NOTE: This is the Professional Version. CONSUMERS: Click here for the Consumer Version ...
merckmanuals.com/en-pr/professional/gynecology-and-obstetrics/infertility/abnormal-cervical-mucus

*  Pediatric Research - Epigenetic Mechanisms and the Mismatch Concept of the Developmental Origins of Health and Disease

Use of model organisms and in vitro techniques relevant to developmental biology and medicine are acceptable, as are ... Although rare, the incidence of this disorder is increased in offspring conceived by assisted reproductive techniques (. 37). ... 41). Exposure of pregnant women to diethylstilbestrol led to a marked increase in reproductive abnormalities and uterine ... Arnaud P, Feil R 2005 Epigenetic deregulation of genomic imprinting in human disorders and following assisted reproduction. ...
nature.com/pr/journal/v61/n5-2/full/pr2007124a.html?foxtrotcallback=true&error=cookies_not_supported&code=c530a89d-b73e-4663-a412-80d3b878c7cb

*  Future Developments to Minimize ART Risks.

The most important complications of assisted reproductive techniques are the risk of ovarian hyperstimulation syndrome (OHSS) ... 23759305 - Maternal, perinatal and long-term outcomes after assisted reproductive techniques (art).... 21806575 - A case study ... The most important complications of assisted reproductive techniques are the risk of ovarian hyperstimulation syndrome (OHSS) ... Previous Document: Ethical considerations arising from the use of assisted reproductive technologies.. Next Document: Small ...
biomedsearch.com/nih/Future-Developments-to-Minimize-ART/22549715.html

*  Sandra L Hrometz

Lastly, new variations of traditional assisted reproductive techniques will be presented.... *. Use of sildenafil for female ...
https://labome.org/expert/usa/ohio/hrometz/sandra-l-hrometz-1383343.html

*  AAAOM Expo 2008 International Oriental Medicine Conference

TCM & Assisted Reproductive Techniques Brown, Magarelli, See, Horn. (format: CD) more... 3 $30.00 set(s) ...
conferencerecording.com/aaaListTapes.asp?CID=AOM28

*  Male infertility Causes - Mayo Clinic

Often in these cases sperm can still be retrieved for use in assisted reproductive techniques. ... and may potentially improve outcomes when using assisted reproductive techniques such as in vitro fertilization. ... In: Yen & Jaffe's Reproductive Endocrinology. 7th ed. Philadelphia, Pa.: Saunders Elsevier; 2014. www.clinicalkey.com. Accessed ... You must produce healthy sperm. Initially, this involves the growth and formation of the male reproductive organs during ...
mayoclinic.org/diseases-conditions/male-infertility/basics/causes/con-20033113

*  JAYPEE BROTHERS

Manual on Advanced Infertility & Assisted Reproductive Techniques. Hrishikesh D Pai Manual On Antepartum Fetal Surveillance ... Manual of Assisted Reproductive Technologies and Clinical Embryology. Pankaj Talwar Manual of Cytogenetics in Reproductive ... Atlas of Human Assisted Reproductive Technologies. Mangala Telang Atlas of Laparoscopic and Robotic Urologic Oncological ... Reproductive and Child Health Care(FOGSI). DK Dutta Reproductive Medicine: Challenges, Solutions and Breakthroughs. Sulbha ...
jaypeebrothers.com/pgSpecialityDetails.aspx?criteria=Obstetrics and Gynaecology&nos=1

*  Discover The REAL Reasons For Infertility Here! | Uber Articles

All it does is suggest assisted reproductive techniques such as IVF and ICSI. Sad but true, their success rate is just 33% and ... the conditions are not extremely sterile and this is quite dangerous as the entire reproductive system of the woman may be ...
uberarticles.com/women/discover-the-real-reasons-for-infertility-here

*  JOHN SMEATON, DIRECTOR OF SPUC: 05/01/2009 - 06/01/2009

"NPT is offered to couples as an 'ethical alternative' to assisted reproductive techniques ... There is no egg selection, no ... A number of Peers made speeches on the subject of assisted suicide. Among those speaking in favour of assisted suicide were two ... Our efforts to assist those who are tempted to do what is always and everywhere wrong or are suffering from the effects of ... Anthony Ozimic of SPUC, who assists me with my blog, is an Australian, and he tells me:. "The history of modern Australia to ...
spuc-director.blogspot.com/2009_05_03_archive.html

*  Conceiving issues | GulfNews.com

If couples can manage these psychological issues, assisted-reproductive techniques (ART) can help women who can't conceive ... Artificial reproductive treatment. In vitro fertilisation: It is ideal for women who have blocked/damaged fallopian tubes but ... Though exact statistics is not available, it's estimated that one in six couples now need assisted conception to become parents ... The precise treatment depends on the couple, considering their age, the duration of infertility and the reproductive health of ...
gulfnews.com/gn-focus/conceiving-issues-1.899095

*  HIV & AIDS Information :: HATIP #98, 21st December 2007 - Managing meningitis in people with HIV in resource-limited settings:...

Lumbar puncture technique. Supplied by Dr. Douglas Wilson at Edendale Hospital in KwaZulu Natal, South Africa:. *Give morphine ... Francois Venter of the Reproductive Health and HIV Research Unit, of the University of the Witwatersrand, Johannesburg, South ... The delays in disability grant applications affect availability of resources which assist the family. ... Hakim called "fairly crude diagnostic techniques" (see section on diagnosis), 200 cases were confirmed as meningitis: 89 (45%) ...
aidsmap.com/Managing-meningitis-in-people-with-HIV-in-resource-limited-settings-a-clinical-review/page/1256395/

Reproductive technology: Reproductive technology (RT) encompasses all current and anticipated uses of technology in human and animal reproduction, including assisted reproductive technology, contraception and others.Male infertilityNatural cycle in vitro fertilization: Natural Cycle IVF is in vitro fertilisation (IVF) using either of the following procedures:Prenatal nutrition: Nutrition and weight management before and during :pregnancy has a profound effect on the development of infants. This is a rather critical time for healthy fetal development as infants rely heavily on maternal stores and nutrient for optimal growth and health outcome later in life.Pregnancy rate: Pregnancy rate is the success rate for getting pregnant. It is the percentage of all attempts that leads to pregnancy, with attempts generally referring to menstrual cycles where insemination or any artificial equivalent is used, which may be simple artificial insemination (AI) or AI with additional in vitro fertilization.Embryo transfer: Embryo transfer refers to a step in the process of assisted reproduction in which embryos are placed into the uterus of a female with the intent to establish a pregnancy. This technique (which is often used in connection with in vitro fertilization (IVF)), may be used in humans or in animals, in which situations the goals may vary.AzoospermiaSelective reduction: Selective reduction (or multifetal pregnancy reduction or MFPR) is the practice of reducing the number of fetuses in a multifetal pregnancy, say quadruplets, to a twin or singleton pregnancy.Spermiogenesis: Spermiogenesis is the final stage of spermatogenesis, which sees the maturation of spermatids into mature, motile spermatozoa. The spermatid is more or less circular cell containing a nucleus, Golgi apparatus, centriole and mitochondria.Twin reversed arterial perfusionOvarian hyperstimulationOvarian tissue cryopreservation: Ovarian tissue cryopreservation is cryopreservation of tissue of the ovary of a female.Hyperactivation: Hyperactivation is a type of sperm motility. Hyperactivated sperm motility is characterised by a high amplitude, asymmetrical beating pattern of the sperm tail (flagellum).Semen quality: Semen quality is a measure of the ability of semen to accomplish fertilization. Thus, it is a measure of fertility in a man.Sperm: Sperm is the male reproductive cell and is derived from the Greek word (σπέρμα) sperma (meaning "seed"). In the types of sexual reproduction known as anisogamy and its subtype oogamy, there is a marked difference in the size of the gametes with the smaller one being termed the "male" or sperm cell.Unexplained infertility: Unexplained infertility is infertility that is idiopathic in the sense that its cause remains unknown even after an infertility work-up, usually including semen analysis in the man and assessment of ovulation and fallopian tubes in the woman.merckmanuals > Unexplained Infertility Last full review/revision November 2008 by Robert W.Oocyte selection: Oocyte selection is a procedure that is performed prior to in vitro fertilization, in order to use oocytes with maximal chances of resulting in pregnancy. In contrast, embryo selection takes place after fertilization.

(1/1018) Sperm banking and assisted reproduction treatment for couples following cancer treatment of the male partner.

In recent years, the survival of young males suffering from cancer has been improved. Development of new techniques such as IVF and intracytoplasmic sperm injection enables even low quality spermatozoa to be used successfully. It is possible therefore to preserve fertility potential of cancer patients before embarking on adjuvant chemotherapy and radiotherapy. Recognizing the importance of protecting the fertility potential of these young males, we present our recommendations for sperm cryopreservation based on the 11 year experience of Bourn Hall and the British Joint Council for Clinical Oncology consultation report. This paper discusses the options available for patients who recover from cancer to become fathers. In many cases patients are concerned about possible abnormalities and teratogenic risks to their future children who have been conceived naturally or by fertility treatment. The data available in the literature may reassure the medical community that there is no such increased risk. However, due to the relatively small number of children born after such treatment, a long-term follow-up is required. There is an ongoing debate regarding the justification for the programme due to the small number of patients who make use of their banked spermatozoa. The authors believe in the importance of protecting the fertility potential of cancer patients, enabling them to father their genetic children in the future while fighting their illness.  (+info)

(2/1018) Cryopreservation of testicular tissue in young cancer patients.

Cryopreservation of testicular tissue might benefit prepubertal boys who must undergo chemotherapy or radiotherapy. Cryopreservation of testicular tissue and testicular cells for intracytoplasmic sperm injection (ICSI) is feasible and widely applied. Testicular tissue from prepubertal boys can also be frozen, by applying techniques used with other tissues and with testicular tissue from adult men before ICSI. Good results have been obtained when propanediol is used as a cryoprotectant, but glycerol has also been used when freezing testicular tissue. Spermatogonia might also be isolated and cryopreserved as a cell suspension, though practical experience in humans is lacking. Transplantation of the frozen-thawed cells back to the testes after cancer treatment might result in restoration of spermatogenesis. Live offspring have been born to mice after transplantation of fresh, but not cryopreserved, testicular cells. Transplantation is technically feasible also in larger species, but to date no offspring have been born. Spermatogenesis in vitro would be an excellent option for boys with haematological malignancies who carry a risk of relapse after transplantation; however, at present the method is feasible only for the late stages of spermatogenesis.  (+info)

(3/1018) Culture of preimplantation embryos and its long-term effects on gene expression and phenotype.

A growing number of medical, scientific and biotechnological procedures rely on culture of mammalian preimplantation embryos. This review presents currently available data on aberrant offspring development that sometimes arises from commonly applied in-vitro procedures in humans, ruminant species and mice. Comparison between mammalian species reveals similarities in the phenotypic abnormalities that are observed at fetal and perinatal stages of development. In particular, aberrant effects on fetal growth have been observed in multiple studies in which serum complemented the preimplantation culture medium. Although it remains to be determined whether there is a common causal mechanism(s) involved, several hypotheses have been put forward to account for the variety of the observed developmental abnormalities. One of these postulates that culture can result in the epigenetic deregulation of developmentally important genes, and that such epigenetic alterations would affect in particular the expression of genes that are subject to genomic imprinting. Imprinted genes play key roles in the control of fetal growth, and altered imprinting can cause growth defects. Some recent in-vitro culture studies on mice and ruminant species now lend support to this hypothesis.  (+info)

(4/1018) Cytoplasmic transfer in assisted reproduction.

This report details the use of cytoplasmic transfer in human oocytes. The introduction of a small amount of ooplasm from a donor oocyte or zygote may alter the function of oocytes, with probable deficiencies. Cytoplasmic transfer from fertile donor oocytes or zygotes into compromised oocytes from patients with recurrent implantation failure after assisted reproduction has now led to the birth of nearly 30 healthy babies worldwide. Transfer of small amounts of cytoplasm probably involves mRNAs, proteins and mitochondria, as well as other factors and organelles. Even though the use of cytoplasmic transfer has been employed in several IVF clinics--and pregnancies have resulted--it is not known definitively whether the physiology of the early embryo is affected. This review outlines the experimental cytoplasmic transfer techniques and postulates the future impact in assisted reproduction.  (+info)

(5/1018) An analysis of spontaneous hatching in a human endometrial epithelial coculture system: is assisted hatching justified?

PURPOSE: To evaluate spontaneous embryo hatching in an endometrial epithelial coculture system, and compare it with cases where coculture was performed because of maternal age, previous repeated implantation failures, or both. To clarify in which cases assisted hatching would be appropriate. METHODS: Individual human embryos were cocultured on an endometrial epithelial cell monolayer until Day 6. RESULTS: Blastocyst hatching rate at Day 6, depending on maternal age, was 9.1% (age <37 years) and 3.4% (age > or = 37 years). However, blastocyst hatching rates depending on number of previous IVF failures were similar. CONCLUSIONS: Maternal age and previous implantation failures are factors affecting the ability of human embryos to reach the blastocyst stage in coculture. However, assisted hatching is not justified in these populations because of the absence of hatching rate differences between blastocysts obtained from these two groups and the control group.  (+info)

(6/1018) Sonographic diagnosis of pelvic adhesions in patients after ovum pickup.

OBJECTIVE: To evaluate the use of transvaginal sonography for the detection of pelvic adhesions by using clear free fluid in the pouch of Douglas found after ovum pickup. METHODS: A prospective clinical study was performed in an infertility unit of an academic research facility. Sonography was performed in 50 women with infertility 3 days after ovum pickup, and the visceral peritoneum of the uterus, the ovaries, and the fallopian tubes was scanned for possible pelvic adhesions. RESULTS: The serosal surfaces of the uterus, ovaries, and fallopian tubes were successfully observed for the presence of adhesions in 86%, 68%, and 20% of the patients, respectively. Improved visualization was associated with an increased amount of pelvic fluid for the uterus (P = .01) but not for the ovaries and fallopian tubes. The amount of fluid in the pelvis correlated with an increased number of retrieved oocytes (P = .07) and a decreased need for manual manipulation to achieve proper imaging of the uterus (P = .001). CONCLUSION: Transvaginal sonography performed in the presence of fluid in the pelvis may show adhesions mainly attached to the uterus and ovaries. Assessment of possible mechanical factors is important in planning treatment of patients with infertility.  (+info)

(7/1018) Six unaffected livebirths following preimplantation diagnosis for spinal muscular atrophy.

Spinal muscular atrophy (SMA) is a severe neurodegenerative autosomal recessive disorder, second only in frequency to cystic fibrosis. In its most severe form, SMA type I (Werdnig-Hoffman), death invariably ensues before age 2 years from respiratory failure or infection. Around 98% of clinical cases of SMA are caused by the homozygous absence of a region of exons 7 and 8 of the telomeric copy of the SMN gene (SMN1) on chromosome 5. We have developed a novel means of preimplantation diagnosis of SMA using a nested polymerase chain reaction (PCR) amplification of exon 7 of SMN, followed by a HinfI restriction digest of the PCR product enabling the important SMN1 gene to be distinguished from the centromeric SMN2 gene which has no clinical phenotype. This method was designed to reduce the likelihood of misdiagnosis. Five couples were treated using this method. Four proceeded to embryo transfer which resulted in six liveborns (one singleton, one twin and one triplet), all free of SMA. Embryo transfer was not performed in one cycle because of PCR contamination.  (+info)

(8/1018) Oocyte comet assay of luteal phase sera from nonpregnant patients after assisted reproductive procedures.

PURPOSE: Serum factors in patients with recurrent spontaneous abortions (RSA) inhibit mouse embryo development in vitro. Serum factors affecting DNA integrity remain to be tested. The null hypothesis was that patient sera do not affect DNA integrity. The objectives were (a) to use the oocyte comet assay to assess DNA damage after exposure to patient sera and (b) to determine the effect of sera from gravidity 0 parity 0 patients to induce DNA apoptosis. METHODS: Luteal phase sera were drawn 1 week after embryo transfer following assisted reproductive procedures. Frozen-thawed hamster zona intact oocytes at metaphase II were incubated in groups of eight in either control medium or medium supplemented with 50% patient serum for 1.5 h at 37 degrees C in room air. The oocytes were fixed, stained in acridine orange, embedded in agarose, lysed, and alkaline electrophoresis performed. The intensities of the digitized fluorescent images were analyzed. RESULTS: The sera of nonpregnant patients (64%) caused significant fragmentation of hamster oocyte DNA when compared with pregnant patient sera. This difference was also observed when adjusted for patient age. Sera of patients that had never been pregnant also resulted in oocyte DNA fragmentation. CONCLUSIONS: The results suggested that sera from patients that did not conceive contained factors that did not support cell growth by causing DNA fragmentation and apoptosis. The level of the apoptotic factors varied from cycle to cycle. However, more studies are needed to determine if the sera factors actually reach the uterine environment to cause the undesirable effects.  (+info)



reproduction


  • The last decades have witnessed expansion of assisted reproduction techniques (ART) both in terms of advanced technology used and its availability. (springer.com)
  • Embryo reduction of multifetal pregnancies following assisted reproduction treatment: a modification of the transvaginal ultrasound-guided technique. (biomedsearch.com)
  • First-trimester transvaginal embryo reduction is an effective alternative for the management of multifetal pregnancy in assisted reproduction. (biomedsearch.com)