Neoplastic Stem Cells: Highly proliferative, self-renewing, and colony-forming stem cells which give rise to NEOPLASMS.Stem Cells: Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.Hematopoietic Stem Cells: Progenitor cells from which all blood cells derive.Stem Cell Transplantation: The transfer of STEM CELLS from one individual to another within the same species (TRANSPLANTATION, HOMOLOGOUS) or between species (XENOTRANSPLANTATION), or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). The source and location of the stem cells determines their potency or pluripotency to differentiate into various cell types.Embryonic Stem Cells: Cells derived from the BLASTOCYST INNER CELL MASS which forms before implantation in the uterine wall. They retain the ability to divide, proliferate and provide progenitor cells that can differentiate into specialized cells.Adult Stem Cells: Cells with high proliferative and self renewal capacities derived from adults.Pluripotent Stem Cells: Cells that can give rise to cells of the three different GERM LAYERS.Hematopoietic Stem Cell Transplantation: Transfer of HEMATOPOIETIC STEM CELLS from BONE MARROW or BLOOD between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). Hematopoietic stem cell transplantation has been used as an alternative to BONE MARROW TRANSPLANTATION in the treatment of a variety of neoplasms.Stem Cell Niche: A particular zone of tissue composed of a specialized microenvironment where stem cells are retained in a undifferentiated, self-renewable state.Neural Stem Cells: Self-renewing cells that generate the main phenotypes of the nervous system in both the embryo and adult. Neural stem cells are precursors to both NEURONS and NEUROGLIA.Induced Pluripotent Stem Cells: Cells from adult organisms that have been reprogrammed into a pluripotential state similar to that of EMBRYONIC STEM CELLS.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Multipotent Stem Cells: Specialized stem cells that are committed to give rise to cells that have a particular function; examples are MYOBLASTS; MYELOID PROGENITOR CELLS; and skin stem cells. (Stem Cells: A Primer [Internet]. Bethesda (MD): National Institutes of Health (US); 2000 May [cited 2002 Apr 5]. Available from: Stem Cell Transplantation: Transfer of MESENCHYMAL STEM CELLS between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS).Stem Cell Factor: A hematopoietic growth factor and the ligand of the cell surface c-kit protein (PROTO-ONCOGENE PROTEINS C-KIT). It is expressed during embryogenesis and is a growth factor for a number of cell types including the MAST CELLS and the MELANOCYTES in addition to the HEMATOPOIETIC STEM CELLS.Mesenchymal Stromal Cells: Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.

*  Evaluation of cancer stem cell migration using compartmentalizing microfluidic devices and live cell imaging.

Neoplastic Stem Cells / pathology*. Single-Cell Analysis / instrumentation*, methods. Tumor Cells, Cultured. ... If required after cell counting, additional stem cell medium is added to the BTSC suspension to give a cell density of 20,000 ... Cancer Stem Cells-Perspectives on Current Status and Future DirectionsAACR Workshop on Cancer Stem Cells. Cancer ResearchYear: ... BTSCs enriched in this manner demonstrate stem cell-like properties, such as expression of stem cell markers (CD133, nestin), ...

*  adult optic nerve glioma 2005:2010[pubdate] *count=100 - BioMedLib™ search engine

Neoplastic Stem Cells / pathology. Optic Nerve Glioma / pathology. *[MeSH-minor] Adult Stem Cells / pathology. Biomarkers / ... Lasky JL 3rd, Choe M, Nakano I: Cancer stem cells in pediatric brain tumors. Curr Stem Cell Res Ther; 2009 Dec;4(4):298-305. ... The adult population presented more cases of basal cell carcinomas (18 cases), squamous cell carciomas (12 cases), meningiomas ... Cell Differentiation. Chemotherapy, Adjuvant. Child. Humans. Surgical Procedures, Operative. *Genetic Alliance. consumer health ...

*  Cytogenetics sub-cluster 0

Chronic myelogenous leukemia (CML) results from the neoplastic transformation of a haematopoietic stem cell. The hallmark ... Chronic myeloid leukemia (CML) is a myeloproliferative disorder characterized by the presence in leukemic stem cells of the ... CML is derived from the hematopoietic stem cells (1) with the Philadelphia chromosome resulting from of a reciprocal ... Chronic myeloid leukemia (CML) is a myeloproliferative disorder characterized by increased proliferation of granulocytic cells ...

*  Variant Philadelphia translocations with different breakpoints in six chronic myeloid leukemia patients / Alti kronik miyeloid...

... is a clonal myeloproliferative neoplasm arising from neoplastic transformation of a pluripotent stem cell. The Philadelphia (Ph ...

*  Additive antitumor activity of dasatinib and Eto in MDA | Open-i

The cells were treated with indicated concentrations of dasatinib and/or 0.1 μM Eto for t ... Additive antitumor activity of dasatinib and Eto in MDA-MB-231 cells. ... the growth of breast cancer cells of the basal B subtype associated with a significant loss of putative cancer stem cell ... dasatinib significantly decreased the proportion of ALDH1-positive cells in the basal B cell lines but not in the other cell ...

*  Identification and localization of KIAA1114 with a nove | Open-i

Neoplastic Stem Cells/metabolism*. Minor. *Animals. *Antibodies, Monoclonal. *Antigens, Surface. *Cell Line, Tumor ... KIAA1114high cells isolated from HCC cell lines displayed TIC-like features with superior functional and phenotypic traits ... KIAA1114high cells isolated from HCC cell lines displayed TIC-like features with superior functional and phenotypic traits ... KIAA1114, a full-length protein encoded by the trophinin gene, is a novel surface marker for isolating tumor-initiating cells ...

*  Free Medical Flashcards about WBC Path

neoplastic stem cells home in to secondary hematopoietic organs, esp spleen, leading to splenomegaly - terminal spent phase: ... type of HL with infiltrate of T-cells, eosinophils, plasma cells, benign macrophages with Reed-Sternberg cells; RS cells often ... Neoplastic cells are positive for CD19, CD20, CD23, and CD5; deletions in 13q, 11q, 17p, trisomy 12q?. CLL/SLL. ... neoplastic cell of this disease has 'owl eye' bilobed nucleus w/ central nucleoli surrounded by clear space. Hodgkin lymphoma ( ...

*  Neurocytoma Is a Tumor of Adult Neuronal Progenitor Cells | Journal of Neuroscience

... for the discovery of genes causally involved in the neoplastic transformation of tissue-specific stem and progenitor cells. ... Neurocytoma cells activate the early neural enhancer of the nestin gene. Neural stem and progenitor cells may be recognized by ... 1994) Neural stem cells in the adult mammalian forebrain: a relatively quiescent subpopulation of subependymal cells. Neuron 13 ... 2001a) Nestin-EGFP transgenic mice: visualization of the self-renewal and multipotency of CNS stem cells. Mol Cell Neurosci 17: ...

*  Spatiotemporal analysis of transcription regulatory networks in cancer | National Cancer Center Reseach Institute

In Vitro and In Vivo Correlates of Physiologic and Neoplastic Human Fallopian Tube Stem Cells. J Pathol., 238(4): 519-530. ( ... 2015) (Research Highlight in Cell Research and Preview in Cell Stem Cell) PMID: 26040716 ... Distal Airway Stem Cells Render Alveoli in Vitro and During Lung Regeneration Following H1N1 Influenza Infection. Cell, 147, ... Residual embryonic cells as precursors of a Barrett's-like metaplasia. Cell, 145, 1023-1035. (2011) (Featured Article in Cell. ...

*  Pulmonary fibroblasts induce epithelial mesenchymal transition and some characteristics of stem cells in non-small cell lung...

Neoplastic Stem Cells/pathology*. *Tumor Cells, Cultured. LinkOut - more resources. Full Text Sources. *Elsevier Science ... Pulmonary fibroblasts induce epithelial mesenchymal transition and some characteristics of stem cells in non-small cell lung ... Fibroblasts were associated with increased malignant potential and the acquisition of stem cell-like properties in NSCLC tumors ... Subcutaneous co-injection of lung cancer cells and CAFs in mice enhanced tumor growth when compared with cancer cells alone, ...

*  Fludarabine Phosphate, Busulfan, and Anti-Thymocyte Globulin Followed By Donor Peripheral Blood Stem Cell Transplant,...

Cell Transformation, Neoplastic. Neoplastic Processes. Syndrome. Leukemia. Leukemia, Myeloid. Leukemia, Myeloid, Acute. ... When the healthy stem cells from a donor are infused into the patient they may help the patient's bone marrow make stem cells, ... peripheral blood stem cell transplantation Procedure: allogeneic hematopoietic stem cell transplantation Other: laboratory ... before a donor peripheral blood stem cell transplant helps stop the growth of cancer cells. It may also stop the patient's ...

*  RSPO3 expands intestinal stem cell and niche compartments and drives tumorigenesis | Gut

... stem cell zone in hyperplastic and neoplastic lesions (figure 3A). In parallel, we observed a clear increase in the abundance ... stem cells, Paneth cells, non-Paneth cell label-retaining cells and Lgr4+ cells, thus promoting both intestinal stem cell and ... stem cells, Paneth cells, Lgr4+ cells and LRCs. The capacity to stimulate stem cells and niche cells by promoting their Wnt ... Krt19(+)/Lgr5(-) Cells Are Radioresistant Cancer-Initiating Stem Cells in the Colon and Intestine. Cell stem cell 2015;16:627- ...

*  Efficacy of Prophylactic Itraconazole in High-Dose Chemotherapy and Autologous Hematopoietic Stem Cell Transplantation - Full...

Neoplastic Syndromes, Hereditary. Kidney Diseases. Urologic Diseases. Genetic Diseases, Inborn. Retinal Neoplasms. Eye ... aplasia is unavoidable even after autologous hematopoietic stem cells infusion because it takes time for infused stem cells to ... Patients with high risk solid tumors who are going to receive high dose chemotherapy and autologous hematopoietic stem cell ... Efficacy of Prophylactic Itraconazole in High-Dose Chemotherapy and Autologous Hematopoietic Stem Cell Transplantation. This ...

*  Bone Marrow Derived Adult Stem Cells for Acute Anterior Myocardial Infarction - Full Text View -

Neoplastic disease without documented remission within the past 5 years. *Cerebrovascular insult within 3 months ... adult stem cells. bone marrow progenitor cells. bone marrow stem cells. autologous. left ventricular function. intracoronary ... Click here for information on our cardiac stem cell trials Publications automatically indexed to this study by ClinicalTrials. ... Bone Marrow Derived Adult Stem Cells for Acute Anterior Myocardial Infarction (REGEN-AMI). This study is ongoing, but not ...

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Malignant neoplastic diseases treated by bone marrow or stem cell transplantation. Cardiovascular System Chronic heart failure ... Aplastic anemias with bone marrow or stem cell transplantation.. Mental Organic Mental Disorders. Schizophrenic, paranoid and ... Malignant Neoplastic Diseases Soft tissue tumors of the head and neck. Skin. Soft tissue sarcoma. Lymphoma. Leukemia. Multiple ... Sickle cell disease, or one of its variants. Chronic thrombocytopenia (due to any cause). Hereditary telangiectasia. ...

*  IJMS | Free Full-Text | Epidermal Stem Cells in Orthopaedic Regenerative Medicine | HTML

Epidermal stem cells play a critical role via cell replacement, and demonstrate significant translational potential in the ... In this review, we will summarize the biological characteristics of epidermal stem cells, and their potential in orthopedic ... Although controversies and unknown issues remain, epidermal stem cells possess an immune-privileged property in transplantation ... These studies reported various subpopulations and differentiations existing in the epidermal stem cell. ...

*  Coevolution of neoplastic epithelial cells and multilineage stroma via polyploid giant cells during immortalization and...

The cancer stem cell niche: how essential is the niche in regulating stemness of tumor cells? Cell Stem Cell. 2015;16:225-238. ... Cell Stem Cell. 2009;4:440-452. [PubMed]. 27. Hanahan D, Weinberg RA. Hallmarks of cancer: the next generation. Cell. 2011;144: ... Exp Cell Res. 2010;316:2099-2112. [PubMed]. 35. Scaffidi P, Misteli T. In vitro generation of human cells with cancer stem cell ... Endothelial cell differentiation of human breast tumour stem/progenitor cells. J Cell Mol Med. 2009;13:309-319. [PMC free ...

*  Arsenic exposure transforms human epithelial stem/progenitor cells into a cancer stem-like phenotype. - Free Online Library

... poisoning Complications and side effects Heavy metals Health aspects Prostate cancer Care and treatment Risk factors Stem cells ... Arsenic exposure transforms human epithelial stem/progenitor cells into a cancer stem-like phenotype.(Research, Report) by ' ... 2006). Similarly, prenatal arsenic exposure in mice induces or initiates neoplastic lesions throughout the UGS in adulthood ( ... Module map of stem cell genes guides creation of epithelial cancer stem cells. Cell Stem Cell 2:333-344. Yilmaz OH, Valdez R, ...

*  The Notch Receptor and Its Ligands Are Selectively Expressed During Hematopoietic Development in the Mouse - Walker - 2001 -...

Stem Cell Research, 2013, 11, 2, 693. CrossRef. *9. Y.-T. Shih, M.-C. Wang, T.-L. Yang, J. Zhou, D.-Y. Lee, P.-L. Lee, S.-F. ... Modeling Notch Signaling in Normal and Neoplastic Hematopoiesis: Global Gene Expression Profiling in Response to Activated ... All STEM CELLS Journals Search. Clear All Search in. All STEM CELLS Journals. STEM CELLS. STEM CELLS Translational Medicine. ... Brandoch D. Cook, Modeling murine yolk sac hematopoiesis with embryonic stem cell culture systems, Frontiers in Biology, 2014, ...

*  Dysregulation of signaling pathways and putative biomarkers in liver cancer stem cells (Review)

Activated hepatic stellate cells induce tumor progression of neoplastic hepatocytes in a TGF-beta dependent fashion. J Cell ... 3. Liver stem/progenitor cells and liver cancer stem cells. Liver progenitor cells, a type of bipotential cell in human liver ... There are two main potential sources of liver stem cells: adult liver stem/progenitor cells and extrahepatic stem cells. The ... cells (78). Taken together, this information suggests that EpCAM(+) HCC cells represent hepatic stem cells and that these cells ...

*  Expression of ΔNLef1 in mouse epidermis results in differentiation of hair follicles into squamous epidermal cysts and...

Watt, F. M. (2001). Epidermal stem cells. In Stem Cells Biology (ed. D. R. Marshak, R. L. Gardner and D. Gottlieb), CSH ... and that more than one genetic change is necessary for human cells to become neoplastic (Hahn et al., 1999). Single oncogenic ... dermal papilla cells) stimulate the transient proliferation of stem cells in the bulge, whose progeny differentiate into cells ... The cells that give rise to tumours are presumably stem cells that lie in the upper part of the hair follicle, which as ...

*  Myelofibrosis

... more common than secondary myelofibrosis and it results from neoplastic transformation of a multipotent bone marrow stem cell. ... allogeneic hematopoietic stem cell transplantation should be considered. Nonmyeloablative allogeneic stem cell transplantation ... Blood cell morphology is variable. Red blood cells (RBCs) are poikilocytic. Reticulocytosis and polychromatophilia may be ... myelofibrosis is a myeloproliferative disease in which the proliferation of an abnormal type of bone marrow stem cell results ...

*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols

iPSC-like cells expressed stem cell-like characteristics including alkaline phosphatase activity and some stem cell markers. ... However, such iPSC-like cells also proliferated rapidly, became neoplastic, and potentiated cell malignancy at a later stage in ... The induced pluripotent stem cells (iPSC)-like cells were generated from DAOY medulloblastoma cell by introduction of JDP2, and ... Bovine induced pluripotent stem cells are more resistant to apoptosis than testicular cells in response to mono-(2-ethylhexyl) ...

*  Plus it

... that in normal stem cells cannot represent a valuable target whereas mediating a T cell cytotoxicity on neoplastic counterparts ... Melanoma stem-like cells can be eliminated by targeting cancer/testis antigens. Keystone Symposia: Stem Cells and Cancer 2007; ... Identification of a subpopulation of cells with cancer stem cell properties in head and neck squamous cell carcinoma. Proc Natl ... Isolation and in vitro propagation of tumorigenic breast cancer cells with stem/progenitor cell properties. Cancer Res 2005;65: ...

*  Program Members > Yale Cancer Center | Yale School of...

Cell Transformation, Neoplastic; CRISPR-Cas Systems; Genetics; Immunity; Lung Neoplasms; Neoplasm Metastasis; Stem Cells ... Cell Biology; Genetics, Medical; Human Genome Project; Musculoskeletal Diseases; Skin and Connective Tissue Diseases ...

Stem cell theory of aging: The stem cell theory of aging is a new theory which was formulated by several scientists and which postulates that the aging process is the result of the inability of various types of stem cells to continue to replenish the tissues of an organism with functional differentiated cells capable of maintaining that tissue's (or organ's) original function. Damage and error accumulation in genetic material is always a problem for systems regardless of the age.Renal stem cell: Renal stem cells are self-renewing, multipotent stem cells which are able to give rise to all the cell types of the kidney. It is involved in the homeostasis and repair of the kidney, and holds therapeutic potential for treatment of kidney failure.Myeloid: The term myeloid (myelogenous) is an adjective that can refer to a progenitor cell for granulocytes, monocytes, erythrocytes, or platelets. Myeloid can be distinguished from the lymphoid progenitor cells that give rise to B cells and T cells.Human embryonic stem cells clinical trials: ==Human Embryonic Stem Cell Clinical Trials==Pluripotency (biological compounds): The pluripotency of biological compounds describes the ability of certain substances to produce several distinct biological responses. Pluripotent is also described as something that has no fixed developmental potential, as in being able to differentiate into different cell types in the case of pluripotent stem cells.Hematopoietic stem cell transplantationGliogenesis: Gliogenesis is the generation of non-neuronal glia populations derived from multipotent neural stem cells.Stemgent: Stemgent is an American privately funded biotech company focused on providing reagents and technology developed by some of the world's leading stem cell scientists. Founded in 2008, Stemgent has two fully operational facilities in both San Diego, California and Cambridge, Massachusetts.AncestimSB-431542

(1/3999) Differential diagnostic significance of the paucity of HLA-I antigens on metastatic breast carcinoma cells in effusions.

Distinction between benign reactive mesothelial cells and metastatic breast adenocarcinoma cells in effusions from patients with a known prior history of breast cancer is not the easiest task in diagnostic pathology. Here, we report the usefulness of testing the expression of class I HLA antigens (HLA A, B, C) in this respect. Cytospins were prepared from effusions of patients without the history of breast cancer (5 cases) and from effusions of patients with infiltrating ductal carcinoma (11 cases). Three effusions from cancerous patients were not malignant cytologically. The expression of HLA-A, B, C, HLA-DR and beta2-microglobulin as well as the macrophage antigen, CD14, was evaluated by immunocytochemistry. In 10 of 11 effusions the cytologically malignant cells expressed very weak or undetectable HLA-A,B,C as compared to the mesothelial cells and macrophages. The paucity of expression of HLA-A, B, C was detectable in those 3 cases where a definitive cytological diagnosis of malignancy could not be established. In contrast, mesothelial cells and macrophages from all samples were uniformly and strongly positive for both HLA-A, B, C and beta2-microglobulin. We conclude that the paucity of HLA-I antigens provides a marker helpful in distinguishing metastatic breast carcinoma cells from reactive mesothelial cells in effusions.  (+info)

(2/3999) Cytokine treatment or accessory cells are required to initiate engraftment of purified primitive human hematopoietic cells transplanted at limiting doses into NOD/SCID mice.

Little is known about the cell types or mechanisms that underlie the engraftment process. Here, we have examined parameters affecting the engraftment of purified human Lin-CD34+CD38- normal and AML cells transplanted at limiting doses into NOD/SCID recipients. Mice transplanted with 500 to 1000 Lin-CD34+CD38- cord blood (CB) or AML cells required the co-transplantation of accessory cells (ACs) or short-term in vivo cytokine treatment for engraftment, whereas transplantation of higher doses (>5000 Lin-CD34+CD38- cells) did not show these requirements suggesting that ACs are effective for both normal and leukemic stem cell engraftment in this model. Mature Lin+CD34- and primitive Lin-CD34+CD38+ cells were capable of acting as ACs even though no repopulating cells are present. Cytokine treatment of NOD/SCID mice could partially replace the requirement for co-transplantation of AC. Furthermore, no difference was seen between the percentage of engrafted mice treated with cytokines for only the first 10 days after transplant compared to those receiving cytokines for the entire time of repopulation. Surprisingly, no engraftment was detected in mice when cytokine treatment was delayed until 10 days posttransplant. Together, these studies suggest that the engraftment process requires pluripotent stem cells plus accessory cells or cytokine treatment which act early after transplantation. The NOD/SCID xenotransplant system provides the means to further clarify the processes underlying human stem cell engraftment.  (+info)

(3/3999) Autologous transplantation of chemotherapy-purged PBSC collections from high-risk leukemia patients: a pilot study.

We have recently demonstrated that the combination of the alkylating agent nitrogen mustard (NM) and etoposide (VP-16) is capable of eliminating, ex vivo, leukemic cells contaminating PBSC collections and this is associated with a significant recovery of primitive and committed hematopoietic progenitor cells. Based on these data a pilot study on autologous transplantation of NM/VP-16 purged PBSC for high-risk leukemic patients was recently initiated. Twelve patients (seven females and five males) with a median age of 46 years (range 18-57) have been treated. Two patients had acute myeloblastic leukemia (AML) resistant to conventional induction treatment, four patients had secondary AML in I complete remission (CR), one patient was in II CR after failing a previous autologous BM transplantation, while two additional AML individuals were in I CR achieved after three or more cycles of induction treatment. Two patients with high-risk acute lymphoblastic leukemia (ALL) in I CR and one patient with mantle cell lymphoma and leukemic dissemination were also included. Eight patients showed karyotypic abnormalities associated with a poor clinical outcome. The mobilizing regimens included cytosine arabinoside and mitoxantrone with (n = 6) or without fludarabine (n = 3) followed by subcutaneous administration of G-CSF (5 microg/kg/day until the completion of PBSC collection) and G-CSF alone (n = 3) (15 microg/kg/day). A median of two aphereses (range 1-3) allowed the collection of 7.2 x 10(8) TNC/kg (range 3.4-11.5), 5 x 10(6) CD34+ cells/kg (range 2.1-15.3) and 9.2 x 10(4) CFU-GM/kg (0.3-236). PBSC were treated with a constant dose of 20 microg of VP-16/ml and a median individual-adjusted dose (survival < or = 5% of steady-state BM CFU-GM) of NM of 0.7 microg/ml (range 0.25-1.25). Eleven patients were reinfused after busulfan (16 mg/kg) and Cy (120 mg/kg) conditioning with a median residual dose of 0.3 x 10(4) CFU-GM/kg (0-11.5). The median time to neutrophil engraftment (>0.5 x 10(9)/l) for evaluable patients was 25 days (range 12-59); the median time to platelet transfusion independence (>20 and >50 x 10(9)/l) was 40 days (18-95) and 69 days (29-235), respectively. Hospital discharge occurred at a median of 25 days (18-58) after stem cell reinfusion. Four individuals are alive in CR (n = 3) or with residual nodal disease (n = 1 lymphoma patient) with a follow-up of 32, 26, 3 and 14 months, respectively. Seven patients died due to disease progression or relapse (n = 5) or extrahematological transplant toxicity (n = 2). Our data suggest that pharmacological purging of leukapheresis collections of leukemic patients at high-risk of relapse is feasible and ex vivo treated cells reconstitute autologous hematopoiesis.  (+info)

(4/3999) Human immunodeficiency virus-associated Hodgkin's disease derives from post-germinal center B cells.

Human immunodeficiency virus-associated Hodgkin's disease (HIV-HD) displays several peculiarities when compared with HD of the general population. These include overrepresentation of clinically aggressive histologic types and frequent infection of Reed-Sternberg (RS) cells by Epstein-Barr virus (EBV). Recently, we have reported that the histogenesis of HD of the general population may be assessed by monitoring the expression pattern of BCL-6, a transcription factor expressed in germinal center (GC) B cells, and of CD138/syndecan-1 (syn-1), a proteoglycan associated with post-GC, terminal B-cell differentiation. In this study, we have applied these two markers to the study of HIV-HD histogenesis and correlated their expression status to the virologic features of this disease. We have found that RS cells of all histologic categories of HIV-HD consistently display the BCL-6(-)/syn-1(+) phenotype and thus reflect post-GC B cells. Although BCL-6(-)/syn-1(+) RS cells of HIV-HD express CD40, they are not surrounded by CD40 ligand-positive (CD40L+) reactive T lymphocytes, which, in HD of the general population, are thought to regulate the disease phenotype through CD40/CD40L interactions. Conversely, RS cells of virtually all HIV-HD express the EBV-encoded latent membrane protein 1 (LMP1), which, being functionally homologous to CD40, may contribute, at least in part, to the modulation of the HIV-HD phenotype.  (+info)

(5/3999) An alternatively spliced form of CD79b gene may account for altered B-cell receptor expression in B-chronic lymphocytic leukemia.

Several functional anomalies of B-chronic lymphocytic leukemia (B-CLL) cells may be explained by abnormalities of the B-cell receptor (BCR), a multimeric complex formed by the sIg homodimer and the noncovalently bound heterodimer Igalpha/Igbeta (CD79a/CD79b). Because the expression of the extracellular Ig-like domain of CD79b has been reported to be absent in the cells of most CLL cases, we have investigated the molecular mechanisms that may account for this defect. Peripheral blood lymphocytes (PBL) from 50 patients and two cell lines (MEC1, MEC2) obtained from the PBL of one of them were studied. MEC1, MEC2, and 75% of CLL cases did not express detectable levels of the extracellular Ig-like domain of CD79b, which was nevertheless present in greater than 80% CD19(+) cells from normal donors. In healthy subjects the expression of CD79b was equally distributed in CD5(+) and CD5(-) B-cell subsets. Reverse transcription-polymerase chain reaction (RT-PCR) analysis of CD79b RNA from all patients and from MEC1 and MEC2 cell lines consistently yielded two fragments of different size (709 bp and 397 bp). The 709-bp band corresponds to CD79b entire transcript; the 397-bp band corresponds to an alternatively spliced form lacking exon 3 that encodes the extracellular Ig-like domain. Both fragments were also visible in normal PBL. The expression of the 397-bp fragment was increased in normal activated B cells, while no difference was seen between CD5(+) and CD5(-) B cells. To obtain a more accurate estimate of the relative proportions of the two spliced forms, a radioactive PCR was performed in 13 normal and 22 B-CLL samples and the results analyzed using a digital imager. The mean value of the CD79b to the CD79b internally deleted ratio was 0.64 +/- 0.20 SD in normal donors and 0.44 +/- 0.27 SD in B-CLL (P =.01). Direct sequencing of 397-bp RT-PCR products and of genomic DNA corresponding to exon 3 from MEC1, MEC2, their parental cells, and five fresh B-CLL samples did not show any causal mutation. Single-strand conformation polymorphism analysis of exon 3 performed in 18 additional B-CLL cases showed a single abnormal shift corresponding to a TGT --> TGC polymorphic change at amino acid 122. We propose a role for the alternative splicing of CD79b gene in causing the reduced expression of BCR on the surface of B-CLL cells. As normal B cells also present this variant, the mechanism of CD79b posttranscriptional regulation might reflect the activation stage of the normal B cell from which B-CLL derives.  (+info)

(6/3999) Feasibility of immunotherapy of relapsed leukemia with ex vivo-generated cytotoxic T lymphocytes specific for hematopoietic system-restricted minor histocompatibility antigens.

Allogeneic bone marrow transplantation (BMT) is a common treatment of hematologic malignancies. Recurrence of the underlying malignancy is a major cause of treatment failure. Donor-derived cytotoxic T lymphocytes (CTLs) specific for patients' minor histocompatibility antigens (mHags) play an important role in both graft-versus-host disease (GVHD) and graft-versus-leukemia (GVL) reactivities. mHags HA-1 and HA-2 induce HLA-A*0201-restricted CTLs in vivo and are exclusively expressed on hematopoietic cells, including leukemic cells and leukemic precursors, but not on fibroblasts, keratinocytes, or liver cells. The chemical nature of the mHags HA-1 and HA-2 is known. We investigated the feasibility of ex vivo generation of mHag HA-1- and HA-2-specific CTLs from unprimed mHag HA-1- and/or HA-2-negative healthy blood donors. HA-1 and HA-2 synthetic peptide-pulsed dendritic cells (DCs) were used as antigen-presenting cells (APC) to stimulate autologous unprimed CD8(+) T cells. The ex vivo-generated HA-1- and HA-2-specific CTLs efficiently lyse leukemic cells derived from acute myeloid leukemia (AML) and acute lymphoid leukemia (ALL) patients. No lytic reactivity was detected against nonhematopoietic cells. Sufficient numbers of the CTLs can be obtained for the adoptive immunotherapy purposes. In conclusion, we present a feasible, novel therapy for the treatment for relapsed leukemia after BMT with a low risk of GVHD.  (+info)

(7/3999) Evaluation of trisomy 12 by fluorescence in situ hybridization in peripheral blood, bone marrow and lymph nodes of patients with B-cell chronic lymphocytic leukemia.

BACKGROUND AND OBJECTIVE: Trisomy 12 is the most common numerical chromosomal aberration in patients with B-cell chronic lymphocytic leukemia (B-CLL). Fluorescence in situ hybridization (FISH) has improved the detection of this cytogenetic abnormality and has made detection possible in all phases of the cell cycle. The presence of the trisomy 12 positive (+12) cell population has generally been investigated in leukemic cells obtained from the peripheral blood of CLL patients. To ascertain whether trisomy 12 is expressed homogeneously in cells of different hemopoietic tissues, we applied FISH to lymph node, peripheral blood and bone marrow samples obtained simultaneously from 23 untreated B-CLL patients. DESIGN AND METHODS: Twenty-three newly diagnosed patients with B-CLL, 15 in stage B and 8 in stage C, were included in the present study. Peripheral blood smears, bone marrow aspirate smears and lymph node touch imprints were collected from each patient at diagnosis. Cytologic preparations were examined by light microscopy in order to assess the lymphocyte morphology. Immunophenotyping was performed by cytofluorimetric analysis of the peripheral blood, bone marrow and lymph node mononuclear cell suspensions. The diagnosis was supported in all cases by histologic findings in bone marrow biopsy and lymph node biopsy specimens. Fluorescence in situ hybridization was performed on smears of blood and aspirated bone-marrow and lymph node touch imprints obtained by fresh tissue apposition. RESULTS: In 6 of the 23 cases (26%) trisomy 12 was clearly present in all tissues examined. A comparative analysis of the three different hemopoietic tissues was performed. A higher percentage of leukemic CD5+CD23+ cells was detected in lymph nodes than in peripheral blood and bone marrow. A significantly higher proportion of trisomic cells was observed in lymph nodes samples than in peripheral blood or bone marrow smears of trisomy 12 positive CLL patients. INTERPRETATION AND CONCLUSIONS: Several previous reports show that only a proportion of malignant B-CLL cells carry trisomy 12 when analyzed by interphase FISH. The higher proportion of +12 cells in lymph nodes than in peripheral blood or bone marrow of CLL patients with trisomy 12 could reflect different cell distributions in different tissues, or lymph node specific tropism, or proliferative advantage in selected tissue. At present, the role of trisomy 12 in the pathogenesis of lymphoproliferative disorders is unclear.  (+info)

(8/3999) Time sequential chemotherapy for primary refractory or relapsed adult acute myeloid leukemia: results of the phase II Gemia protocol.

BACKGROUND AND OBJECTIVE: High-dose cytarabine (HDAra-C), mitoxantrone and etoposide are the mainstay of several active regimens against relapsed or refractory acute myelogenous leukemia (AML). We designed a phase II study to assess the efficacy and side effects of a time sequential application of mitoxantrone plus intermediate-dose Ara-C followed by HDAra-C plus etoposide (GEMIA) in adult patients with refractory or relapsed AML. DESIGN AND METHODS: Patients with refractory or relapsed AML were eligible for GEMIA salvage therapy, which comprised mitoxantrone 12 mg/m2/day on days 1-3, Ara-C 500 mg/m2/day as a 24-hour continuous infusion on days 1-3, followed by HDAra-C 2 g/m2/12-hourly on days 6-8 and etoposide 100 mg/m2/12-hourly on days 6-8. Granulocyte colony-stimulating factor was started on day 14. In patients above the age of 55 the dose of Ara-C in the first sequence (days 1-3) was reduced to 250 mg/m2. RESULTS: Twenty patients were included, of whom 12 achieved complete remission after GEMIA (60%, 95% CI 40-80%), one was refractory and five died early from infection. Two additional patients achieved partial remission after GEMIA and complete remission after consolidation chemotherapy, for a final CR rate of 70% (95% CI 48-88%). Neutrophils recovered at a median of 27 days (range, 22-43) and platelets 46 days (range, 25-59) after the start of treatment. The median duration of remission was 133 days (range, 36-417+) whereas overall survival time lasted for a median of 153 days (range, 13-554+). Treatment-associated toxicity was comprised predominantly of infection, mucositis and diarrhea that reached World Health Organization grades III-V in 40%, 40% and 30% of patients, respectively. Despite the intention to rapidly proceed to a hematopoietic stem cell transplant in patients in remission, only five patients reached the transplant. INTERPRETATION AND CONCLUSIONS: The GEMIA time sequential chemotherapy regimen appears effective in obtaining remissions in refractory and relapsed adult AML. The high toxicity seen, however, suggests that its design is amenable to further improvements, especially in more elderly patients. Since remissions are short-lived, more innovative post-remission strategies are needed.  (+info)

pluripotent stem

  • Chronic myeloid leukemia (CML) is a clonal myeloproliferative neoplasm arising from neoplastic transformation of a pluripotent stem cell. (
  • Recently, several pluripotent stem cell populations from the follicle bulge, expressing characteristic neural crest cell markers, were named epidermal-resident neural crest stem cells (EPI-NCSCs) [ 8 - 10 ] or as follicle pluripotent stem cells (fPSCs) [ 11 - 14 ]. (


  • The interactions of aberrant cell migratory mechanisms and the tumor microenvironment likely differentiate cancer from normal cells. (
  • Recent work suggests that a small subpopulation of cells within GBM, the brain tumor stem cell (BTSC), may be responsible for therapeutic resistance and recurrence. (
  • KIAA1114, a full-length protein encoded by the trophinin gene, is a novel surface marker for isolating tumor-initiating cells of multiple hepatocellular carcinoma subtypes. (
  • Identification of novel biomarkers for tumor-initiating cells (TICs) is of critical importance for developing diagnostic and therapeutic strategies against cancers. (
  • This strategy of comparing the gene expression of tumor cells to that of the purified native progenitors from which they derive may provide a focused approach to identifying transcripts important to stem and progenitor cell oncogenesis. (
  • In the process of cancer initiation and progression, various types of cellular stress such as inflammation cause genomic mutations including single nucleotide polymorphisms and copy number variations in oncogenes and tumor suppressors, as well as epigenetic changes, in multiple stages via pre-neoplastic lesions. (
  • The purpose of this study was to clarify the role of fibroblasts in tumor progression in non-small cell lung cancer (NSCLC). (
  • Subcutaneous co-injection of lung cancer cells and CAFs in mice enhanced tumor growth when compared with cancer cells alone, which was attenuated by administration of SB431542. (
  • The purpose of this study is to investigate whether the prophylactic use of itraconazole is a better option than empirical use of itraconazole in the management (prevention and treatment) of fungal infection associated with high-dose chemotherapy and autologous hematopoietic stem cell transplantation in children with high-risk solid tumor. (
  • In this context, investigators have explored the possible efficacy of high-dose chemotherapy (HDCT) and autologous stem cell transplantation (ASCT) in patients with high-risk or relapsed pediatric solid tumor. (
  • To determine the role of polyploidy in tumor development, we examined the fate of normal mullerian epithelial cells during the immortalization and transformation process by tracing the expression of SV40 large T antigen. (
  • Consistent with tumor-derived CSCs, As-CSCs formed abundant free-floating spheres enriched in CSC-like cells, as confirmed by molecular analysis and the fact that only these floating cells formed xenograft tumors. (
  • Accumulating evidence indicates that cancer stem cells (CSCs) probably arise from normal stem cells (NSCs), or partially differentiated progenitor cells, and that CSCs are likely the driving force in tumor initiation, growth, and progression (Pardal et al. (
  • According to the theory, there is a rare population of stem-like cells in tumor tissue, called liver cancer stem cells (LCSCs), which are responsible for the self-renewal, malignant transformation, metastasis and chemoresistance of HCC. (
  • Currently, all of the cancer cells in a tumor are thought to be responsible for tumor growth. (


  • Pulmonary fibroblasts induce epithelial mesenchymal transition and some characteristics of stem cells in non-small cell lung cancer. (
  • conditioned medium (CM) from fibroblast-induced epithelial mesenchymal transition and acquisition of cancer stem cell-like qualities in lung cancer cells (A549 and NCI-H358), indicating that CM from fibroblasts was biologically active. (
  • Here we show that immortalized or HRAS -transformed mullerian epithelial cells contain a subpopulation of polyploid giant cells that grow as multicellular spheroids expressing hematopoietic markers in response to treatment with CoCl 2 . (
  • The immortalized or transformed epithelial cells can transdifferentiate into stromal cells when transplanted into nude mice. (
  • These results suggest that normal mullerian epithelial cells are intrinsically highly plastic, via the formation of polyploid giant cells and activation of embryonic stem-like program, which work together to promote the coevolution of neoplastic epithelial cells and multiple lineage stromal cells. (
  • A carcinoma is a complex tissue composed of multiple cell types, including epithelial cancer cells as well as stromal cells that include fibroblasts, endothelial cells, and inflammatory cells [ 1 - 4 ]. (
  • Although it is well known that cancer epithelial cells and stromal cells interact extensively to form a critical network that sustains and regulates cancer growth, the development of cancer is generally attributed to clonal proliferation of transformed epithelial cells, resulting from accumulated genetic mutations of either somatic or adult stem cells. (
  • It is generally believed that mutational events lead to uncontrolled proliferation of epithelial cells and that stromal components of tumors are derived from the host's normal mesenchymal stroma or bone marrow-derived mesenchymal stem cells [ 1 , 2 ]. (
  • Arsenic exposure transforms human epithelial stem/progenitor cells into a cancer stem-like phenotype. (
  • METHODS: We continuously exposed the human prostate epithelial stem/progenitor cell line WPE-stem to an environmentally relevant level of arsenic (5 [micro]M) in vitro and determined the acquired cancer phenotype. (
  • When hetero-transplanted, these cells (designated As-CSC) formed highly pleomorphic, aggressive tumors with immature epithelial- and mesenchymal-like cells, suggesting a highly pluripotent cell of origin. (
  • CONCLUSIONS: Arsenite transforms prostate epithelial stem/progenitor cells into CSC-like cells, indicating that it can produce CSCs from a model NSC population. (
  • 2005). Although assumed, proof of the direct emergence of epithelial CSCs from an NSC population is not available, and the identity of the cells that acquire the molecular lesions initiating chemical carcinogenesis remains undefined (Perez-Losada and Balmain 2003). (
  • Numerous LCSC biomarkers have been identified including CD133, epithelial cell adhesion molecule (EpCAM), ABCG2 and CD90, which would contribute to the isolation of LCSCs. (
  • These include CD133, epithelial cell adhesion molecule (EpCAM), ABCG2 and CD90. (


  • The characteristic phenotype and ventricular location of these tumors suggested to us that neurocytoma might arise by the transformation of neuronally biased, transit-amplifying progenitor cells of the adult subependyma. (
  • Fibroblasts were associated with increased malignant potential and the acquisition of stem cell-like properties in NSCLC tumors. (
  • Some studies, however, have demonstrated that endothelial cells can be derived from cancer cells [ 5 - 7 ], and other studies have shown that stromal components of tumors may not be derived entirely from the host's normal mesenchymal stroma or bone marrow [ 8 , 9 ], suggesting that cancer cells are highly plastic and capable of generating stromal cells. (
  • However, recently emerging evidence suggests that there is a rare population of stem-like cells in tumors that determine cancer characteristics. (

progenitor cell

  • Molecular profiling of neurocytoma, and the comparison of its expression profile to that of neural progenitors sorted from adult human lateral ventricular wall, indicated substantial overlap of neurocytoma and native progenitor cell gene expression. (


  • Once high-dose myeloablative chemotherapeutic agents are administered, most hematopoietic cells in bone marrow die and prolonged marrow aplasia is unavoidable even after autologous hematopoietic stem cells infusion because it takes time for infused stem cells to reconstitute sufficient hematopoietic function. (
  • Members of the Notch family of transmembrane receptors are found on primitive hematopoietic precursors, and Notch ligand expression has been demonstrated on the surface of stromal cells, suggesting a role for Notch signaling in mammalian blood cell development. (
  • The current report examines the expression of Notch receptors and their ligands in murine hematopoietic tissues to determine: A) which blood cell lineages in the adult are influenced by Notch activity, and B) whether fetal hematopoiesis in the embryo involves the Notch pathway. (


  • To reduce the chance of infection and therefore, to reduce the morbidity and mortality from severe infection, various prophylactic antibiotics including antibacterial, antifungal, and antiviral agents have been used according to standard guideline in allogeneic stem cell transplantation. (


  • Although controversies and unknown issues remain, epidermal stem cells possess an immune-privileged property in transplantation together with easy accessibility, which is favorable for future clinical application. (


  • KIAA1114high cells isolated from HCC cell lines displayed TIC-like features with superior functional and phenotypic traits compared to their KIAA1114low counterparts, including tumorigenic abilities in xenotransplantation model, in vitro colony- and spheroid-forming capabilities, expression of stemness-associated genes, and migratory capacity. (
  • We noted that CN cells exhibited an antigenic profile typical of neuronal progenitor cells in vivo , yet in vitro generated neurospheres, divided in response to bFGF (basic fibroblast growth factor), activated the neuroepithelial enhancer of the nestin gene, and gave rise to both neuron-like cells and astrocytes. (
  • We found that neurocytoma cells expand as bipotential neuronal-astrocytic progenitor cells in vitro , but are strongly biased toward neurogenesis in vivo . (
  • 2001). Like NSCs, CSCs often form free-floating "spheres" of viable cells in vitro (Dontu et al. (

stromal cells

  • Stromal cells are generally considered to be derived primarily from the host's normal mesenchymal stromal cells or bone marrow. (
  • However, the origins of stromal cells have been quite controversial. (

myeloid cells

  • Flow cytometry demonstrated the presence of Notch1 and Notch2 receptors on bone marrow-derived myeloid cells but not on erythroid cells positive for the marker, Ter-119. (


  • RESULTS: WPE-stem cells rapidly acquired a malignant CSC-like phenotype by 18 weeks of exposure, becoming highly invasive, losing contact inhibition, and hypersecreting matrix metalloproteinase-9. (


  • Immunofluorescent staining revealed expression of stem cell factors OCT4, Nanog, and SOX-2 in spheroid, whereas expression of embryonic stem cell marker SSEA1 was increased in HRAS -transformed cells compared with their immortalized isogenic counterparts. (


  • The present study indicates that dasatinib preferentially inhibits the growth of breast cancer cells of the basal B subtype associated with a significant loss of putative cancer stem cell population. (


  • In this set of studies, we compared the lineal relationships and gene expression patterns of central neurocytoma cells to those of native adult human neural progenitor cells. (
  • By so doing, we tested the hypothesis that neurocytoma may represent a transformed derivative of neuronal progenitor cells of the adult subependyma. (
  • Notch receptors in the adult are most likely utilized by early erythroid precursors and intermediate-stage granulocytes, but not by terminally differentiating cells of either subset. (
  • There are two main potential sources of liver stem cells: adult liver stem/progenitor cells and extrahepatic stem cells. (
  • The adult stem cells reside in the mature liver and can be activated by certain factors. (


  • The undifferentiated cells exhibited self-renewing and multipotent differentiation capacities and gave rise to all component cells of epidermis and hair follicle [ 1 , 2 ]. (
  • 2001). This developing theory is compelling because CSCs and NSCs share key characteristics, including the capacity for limitless self-renewal, which normally allows pluridirectional replenishment via differentiation but during oncogenesis contributes to heterogeneous aberrant cell overgrowth (Pardal et al. (


  • The purpose of this study is to determine whether Intracoronary infusion of autologous bone marrow derived progenitor cells to patients undergoing primary angioplasty for acute anterior myocardial infarction will lead to an improvement in cardiac function greater than that seen by placebo alone. (
  • To demonstrate the effects of autologous bone marrow derived stem cells on cardiac function using cardiac MRI (or cardiac CT), echocardiography and left ventriculography. (
  • To demonstrate the effect of autologous bone marrow derived stem cells in addition to standard care leads to improvement in cardiac function compared to patients saline(placebo) and standard care. (


  • Evolving theory predicts that normal stem cells (NSCs) are transformed into cancer stem cells (CSCs) that then drive oncogenesis. (
  • However, proof of emergence of CSCs induced by arsenic in a stem cell population is not available. (
  • Reya et al ( 9 ) proposed a theory of cancer stem cells (CSCs). (


  • Compared with other established markers of liver TICs, KIAA1114 was unique in that its expression was detected in both alpha fetoprotein (AFP)-positive and AFP-negative hepatocellular carcinoma (HCC) cell lines with the expression levels of KIAA1114 being positively correlated to their tumorigenic potentials. (
  • Expression levels of myofibroblast markers were higher in CAFs than LNFs within 5 passages in the absence of continuing interaction with carcinoma cells. (


  • Liver progenitor cells, a type of bipotential cell in human liver tissue, give rise to both hepatocytes and the biliary tree. (


  • The expression pattern of CN was distinguished from that of native progenitor cells by a cohort of differentially expressed genes potentially involved in both the oncogenesis and phenotypic restriction of neurocytoma. (


  • When the healthy stem cells from a donor are infused into the patient they may help the patient's bone marrow make stem cells , red blood cells , white blood cells , and platelets. (
  • Epidermal stem cells play a critical role via cell replacement, and demonstrate significant translational potential in the treatment of orthopedic injuries and diseases, including treatment for wound healing, peripheral nerve and spinal cord injury, and even muscle and bone remodeling. (


  • Conclusions We provide in vivo evidence that RSPO3 stimulates the crypt stem cell and niche compartments and drives rapid intestinal tumorigenesis. (


  • Sun et al ( 22 ) analyzed different expression patterns of stem-cell markers in HBV-associated cirrhotic livers and in HCC and demonstrated that the stem-like cells possessed tumorigenic capacity and that these cells might be LCSCs. (


  • Due to a limitation in visual inspection and geometrical manipulation, conventional migration assays are restricted to quantifying overall cell populations. (


  • This phase II trial is studying the side effects and how well giving fludarabine phosphate, busulfan, anti-thymocyte globulin followed by donor peripheral blood stem cell transplant , tacrolimus, and methotrexate works in treating patients with myeloid malignancies. (

chemotherapeutic agents

  • To clarify this finding and further investigate combined antitumor effects of dasatinib with cytotoxic agents, a panel of breast cancer cell lines of various subtypes was treated with dasatinib and/or chemotherapeutic agents. (
  • Seven human breast cancer cell lines were treated with dasatinib and/or seven chemotherapeutic agents. (
  • The IC50s of chemotherapeutic agents were not substantially different among the cell lines. (


  • In this review, we will summarize the biological characteristics of epidermal stem cells, and their potential in orthopedic regenerative medicine. (
  • However, the cancer stem cell (CSC) theory suggests that liver cancer stem cells (LCSCs) may be responsible for the biological characteristics of HCC. (


  • Its neuronal phenotype, along with its typical presentation along the lateral ventricular wall, suggests that neurocytoma originates from subependymal neural progenitor cells. (


  • Isolation of an aggressive subpopulation of migratory cells will enable studies of underlying molecular mechanisms. (


  • A major obstacle for improving patient outcomes is the poor understanding of mechanisms underlying cellular migration associated with aggressive cancer cell invasion, metastasis and therapeutic resistance. (
  • In the last decade, great advances have been made in epidermal stem cell studies at the cellular and molecular level. (


  • The phase live images of cell morphology during migration are recorded over several days. (



  • RSPO3 induced the expansion of Lgr5 + stem cells, Paneth cells, non-Paneth cell label-retaining cells and Lgr4 + cells, thus promoting both intestinal stem cell and niche compartments. (
  • Thus, the follicle-derived stem cells were termed as hair follicle stem cells (HFSCs). (


  • Fibroblasts isolated from surgical exploration were co-cultured with human lung adenocarcinoma cell lines. (


  • The oval cells, located in the canal of Hering, have the ability to differentiate into both hepatocytes and biliary epithelia and are now generally acknowledged to be liver stem/progenitor cells ( 23 ). (


  • This renewability, or the regenerative capacity indicates the existence of stem/progenitor cells, which could potentially be harnessed in regenerative medicine. (


  • Dasatinib decreased the expression levels of phosphorylated Src in all cell lines.Interestingly, dasatinib significantly decreased the proportion of ALDH1-positive cells in the basal B cell lines but not in the other cell lines.A combined use of dasatinib with etoposide additively inhibits their growth. (
  • Dasatinib decreased the expression levels of phosphorylated Src in all cell lines. (


  • In adults, epidermis-resident progenitor cells adopt their fates to fit the renewal of the epidermis, hair follicle, and sebaceous gland and maintain a homeostasis in normal tissue. (
  • Polyploid giant cancer cells (PGCCs) are mononucleated or multinucleated cells with multiple genome copies that can be observed in cancer but that can also play an important role in many normal physiologic and nonmalignant pathologic processes [ 10 - 12 ]. (


  • C) Western blot and flow cytometric analysis for recognition of human and mouse KIAA1114 by Kiatomab and 3-11 mAb using 293T cells and mouse brain tissue. (

cancer cell

  • Therefore, improving therapeutic approaches for GBM require a better understanding of cancer cell migration mechanisms. (


  • However, data from several laboratories have shown that PGCCs are capable of generating daughter cells via budding [ 15 - 20 ]. (


  • Preferential antitumor effect of the Src inhibitor dasatinib associated with a decreased proportion of aldehyde dehydrogenase 1-positive cells in breast cancer cells of the basal B subtype. (
  • Recent studies have suggested that the Src inhibitor dasatinib preferentially inhibits the growth of breast cancer cells of the basal-like subtype. (


  • A discrete population of squamocolumnar junction cells implicated in the pathogenesis of cervical cancer. (


  • Traditionally, PGCCs were considered to be senescent and not able to divide, since multiple mechanisms exist in animal cells to limit polyploid cell growth [ 13 , 14 ]. (


  • In contrast, microfluidic devices permit single cell analysis because of compatibility with modern microscopy and control over micro-environment. (


  • Effects of the treatments on c-Src activation, cell growth, cell cycle, apoptosis and the proportion of aldehyde dehydrogenase (ALDH) 1-positive cells were examined. (
  • The 50%-growth inhibitory concentrations (IC50s) of dasatinib were much lower in two basal B cell lines than those in the other cell lines. (
  • Eto additively inhibited the growth of MDA-MB-157 cells. (
  • Furthermore, the concentration of the transforming growth factor (TGF)-β1 was higher in CM from CAFs as compared with that from LNFs, and phenotypic changes of cancer cells by CM from CAFs were greater than those induced by CM from LNFs. (


  • The combination index of IC50 was 0.3 or 0.4 in the MDA-MB-231 or MDA-MB-157 cell line, respectively, that is, Eto additively enhanced the antitumor effect of dasatinib in these cell lines (Figures 6 and 7). (

Featured Article

  • 2011) (Featured Article in Cell. (